ATE121099T1 - Verfahren zur reinigung von intaktem und korrekt gefaltetem igf-i. - Google Patents
Verfahren zur reinigung von intaktem und korrekt gefaltetem igf-i.Info
- Publication number
- ATE121099T1 ATE121099T1 AT92904088T AT92904088T ATE121099T1 AT E121099 T1 ATE121099 T1 AT E121099T1 AT 92904088 T AT92904088 T AT 92904088T AT 92904088 T AT92904088 T AT 92904088T AT E121099 T1 ATE121099 T1 AT E121099T1
- Authority
- AT
- Austria
- Prior art keywords
- igf
- correctly folded
- intact
- peptide
- folded igf
- Prior art date
Links
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 title abstract 3
- 102000004218 Insulin-Like Growth Factor I Human genes 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 101000599951 Homo sapiens Insulin-like growth factor I Proteins 0.000 abstract 1
- 239000003463 adsorbent Substances 0.000 abstract 1
- 238000005341 cation exchange Methods 0.000 abstract 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 238000011084 recovery Methods 0.000 abstract 1
- 238000002336 sorption--desorption measurement Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Endocrinology (AREA)
- Diabetes (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Detergent Compositions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US64143091A | 1991-01-16 | 1991-01-16 | |
US07/785,171 US5231178A (en) | 1991-01-16 | 1991-10-28 | Method for the purification of intact, correctly-folded insulin-like growth factor-1 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE121099T1 true ATE121099T1 (de) | 1995-04-15 |
Family
ID=27093753
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT92904088T ATE121099T1 (de) | 1991-01-16 | 1992-01-15 | Verfahren zur reinigung von intaktem und korrekt gefaltetem igf-i. |
Country Status (9)
Country | Link |
---|---|
US (1) | US5231178A (de) |
EP (1) | EP0567554B1 (de) |
JP (1) | JPH06505010A (de) |
AT (1) | ATE121099T1 (de) |
AU (1) | AU1194792A (de) |
CA (1) | CA2098183C (de) |
DE (1) | DE69202049T2 (de) |
ES (1) | ES2071488T3 (de) |
WO (1) | WO1992012993A1 (de) |
Families Citing this family (63)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5612198A (en) * | 1990-09-04 | 1997-03-18 | The Salk Institute | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
ATE220105T1 (de) * | 1991-04-01 | 2002-07-15 | Merck & Co Inc | Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung |
US5288931A (en) * | 1991-12-06 | 1994-02-22 | Genentech, Inc. | Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation |
US5451660A (en) * | 1993-12-13 | 1995-09-19 | Genentech, Inc. | Method for purifying polypeptides |
US5446024A (en) * | 1993-12-17 | 1995-08-29 | Genentech, Inc. | Purification of insulin-like growth factor |
DE4405179A1 (de) * | 1994-02-18 | 1995-08-24 | Hoechst Ag | Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken |
WO1996007744A1 (en) * | 1994-09-08 | 1996-03-14 | Chiron Corporation | A method of improved production of insulin-like growth factor |
US5641870A (en) | 1995-04-20 | 1997-06-24 | Genentech, Inc. | Low pH hydrophobic interaction chromatography for antibody purification |
US5650496A (en) * | 1995-04-14 | 1997-07-22 | Cephalon, Inc. | IGF-I purification process |
US6756484B1 (en) * | 1995-04-14 | 2004-06-29 | Cephalon, Inc. | IGF-I purification process |
US7193042B1 (en) | 1995-06-07 | 2007-03-20 | Chiron Corporation | Methods for purifying authentic IGF from yeast hosts |
JP2002514890A (ja) * | 1995-06-07 | 2002-05-21 | カイロン コーポレイション | 酵母宿主から真性のigfを精製するための方法 |
US7071313B1 (en) | 1995-06-07 | 2006-07-04 | Cephalon, Inc. | Methods of purifying authentic IGF from yeast hosts |
SE9504019D0 (sv) | 1995-11-13 | 1995-11-13 | Pharmacia Ab | Method for production of proteins |
US6767892B1 (en) * | 1997-11-07 | 2004-07-27 | Chrion Corporation | Compositions providing for increased IGF-I solubility |
DE69919701T2 (de) | 1998-06-01 | 2005-09-15 | Genentech, Inc., South San Francisco | Abtrennung von antikörper-monomeren von deren multimeren mittels ionaustausch-chromatographie |
JP2001218840A (ja) * | 2000-02-14 | 2001-08-14 | Kanegafuchi Chem Ind Co Ltd | トランスフォーミング増殖因子βの吸着材、吸着除去方法および吸着器 |
US7537938B2 (en) * | 2000-04-28 | 2009-05-26 | Monogram Biosciences, Inc. | Biomarker detection in circulating cells |
US20040067498A1 (en) * | 2000-04-28 | 2004-04-08 | Ahmed Chenna | Detection of nucleic acid sequences by cleavage and separation of tag-containing structures |
US7771929B2 (en) * | 2000-04-28 | 2010-08-10 | Monogram Biosciences, Inc. | Tag library compounds, compositions, kits and methods of use |
AR036400A1 (es) * | 2001-08-30 | 2004-09-08 | Stem Cell Therapeutics Inc | Regulacion combinada de la produccion de celulas nerviosas. |
WO2003024472A2 (en) | 2001-09-14 | 2003-03-27 | Stem Cell Therapeutics Inc. | Prolactin induced increase in neutral stem cell numbers and therapeutical use thereof |
WO2003024471A2 (en) * | 2001-09-18 | 2003-03-27 | Stem Cell Therapeutics Inc. | Effect of growth hormone and igf-1 on neural stem cells and therapeutic application |
US6849394B2 (en) * | 2002-02-21 | 2005-02-01 | Minitube Of America | Compositions comprising reproductive cell media and methods for using such compositions |
CA2492442A1 (en) * | 2002-07-31 | 2004-02-05 | Stem Cell Therapeutics Inc. | Method of enhancing neural stem cell proliferation, differentiation, and survival using pituitary adenylate cyclase activating polypeptide (pacap) |
WO2005075498A1 (en) * | 2004-01-30 | 2005-08-18 | Amgen Inc. | Process for purifying proteins |
JP2007519422A (ja) | 2004-02-02 | 2007-07-19 | アンブレツクス・インコーポレイテツド | 修飾されたヒト四螺旋バンドルポリペプチド及びそれらの使用 |
AU2005211847B2 (en) | 2004-02-13 | 2011-02-24 | Stem Cell Therapeutics Corp. | Use of luteinizing hormone (LH) and chorionic gonadotropin (hCG) for proliferation of neural stem cells and neurogenesis |
MXPA06014684A (es) * | 2004-06-18 | 2007-02-12 | Ambrx Inc | Novedosos polipeptidos de enlace antigeno y sus usos. |
AU2005327906B2 (en) * | 2004-07-21 | 2010-05-13 | Ambrx, Inc. | Biosynthetic polypeptides utilizing non-naturally encoded amino acids |
WO2006069246A2 (en) | 2004-12-22 | 2006-06-29 | Ambrx, Inc. | Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides |
ATE541934T1 (de) * | 2004-12-22 | 2012-02-15 | Ambrx Inc | Zusammensetzungen von aminoacyl-trna-synthetase und verwendungen davon |
SG161210A1 (en) | 2004-12-22 | 2010-05-27 | Ambrx Inc | Methods for expression and purification of recombinant human growth hormone |
AU2005322019B2 (en) * | 2004-12-22 | 2010-08-26 | Ambrx, Inc. | Formulations of human growth hormone comprising a non-naturally encoded amino acid |
BRPI0519430A2 (pt) | 2004-12-22 | 2009-02-10 | Ambrx Inc | hormânio do crescimento humano modificado |
KR20080026135A (ko) * | 2005-06-03 | 2008-03-24 | 암브룩스, 인코포레이티드 | 개선된 인간 인터페론 분자 및 이의 용도 |
MX2008002149A (es) * | 2005-08-18 | 2008-04-22 | Ambrx Inc | Composiciones de arnt y sus usos. |
KR20080074108A (ko) | 2005-09-27 | 2008-08-12 | 스템 셀 테라퓨틱스 코포레이션 | 프로락틴에 의해 조절되는 올리고덴드로사이트 전구체 세포증식 |
PT1954710E (pt) * | 2005-11-08 | 2011-06-07 | Ambrx Inc | Aceleradores para a modificação de aminoácidos nãonaturais e polipeptídeos de aminoácidos não-naturais |
EP2339014B1 (de) * | 2005-11-16 | 2015-05-27 | Ambrx, Inc. | Verfahren und Zusammensetzungen mit nichtnatürlichen Aminosäuren |
KR101423898B1 (ko) * | 2005-12-14 | 2014-07-28 | 암브룩스, 인코포레이티드 | 비-천연 아미노산 및 폴리펩티드를 함유하는 조성물, 비-천연 아미노산 및 폴리펩티드를 포함하는 방법, 및 비-천연 아미노산 및 폴리펩티드의 용도 |
KR20080106976A (ko) * | 2006-03-17 | 2008-12-09 | 스템 셀 테라퓨틱스 코포레이션 | 신경 줄기 세포 증식제 및 신경 줄기 세포 분화제의 연속 투여 방법 |
EP2069396B1 (de) | 2006-09-08 | 2015-10-21 | Ambrx, Inc. | Modifiziertes polypeptid oder fc-gerüste aus humanem plasma und ihre verwendung |
US8420792B2 (en) * | 2006-09-08 | 2013-04-16 | Ambrx, Inc. | Suppressor tRNA transcription in vertebrate cells |
NZ574721A (en) * | 2006-09-08 | 2012-02-24 | Ambrx Inc | Hybrid suppressor trna for vertebrate cells |
BRPI0809583B1 (pt) * | 2007-03-30 | 2022-02-22 | Ambrx, Inc | Polipeptídeo fgf-21 modificado, composição compreendendo o mesmo, método para produzir o referido polipetídeo fgf-21 e célula compreendendo um polinucleotídeo |
EP1988154B1 (de) | 2007-04-30 | 2013-08-21 | Ajinomoto Co., Inc. | Verfahren zur Herstellung des humanen insulinähnlichen Wachstumsfaktors I |
CA2685596A1 (en) * | 2007-05-02 | 2008-11-13 | Ambrx, Inc. | Modified interferon beta polypeptides and their uses |
NZ603812A (en) * | 2007-11-20 | 2014-06-27 | Ambrx Inc | Modified insulin polypeptides and their uses |
SG188143A1 (en) | 2008-02-08 | 2013-03-28 | Ambrx Inc | Modified leptin polypeptides and their uses |
NZ591235A (en) * | 2008-07-23 | 2012-07-27 | Ambrx Inc | Modified bovine g-csf polypeptides comprising non natural amino acid and their uses treating infections such as mastitis |
PL2342223T3 (pl) | 2008-09-26 | 2017-09-29 | Ambrx, Inc. | Zmodyfikowane polipeptydy zwierzęcej erytropoetyny i ich zastosowania |
TR201802361T4 (tr) | 2008-09-26 | 2018-03-21 | Ambrx Inc | Doğal olmayan amino asit replikasyonuna bağımlı mikroorganizmalar ve aşılar. |
NZ600361A (en) | 2009-12-21 | 2014-06-27 | Ambrx Inc | Modified bovine somatotropin polypeptides and their uses |
CN104017063A (zh) | 2009-12-21 | 2014-09-03 | Ambrx公司 | 经过修饰的猪促生长素多肽和其用途 |
US9567386B2 (en) | 2010-08-17 | 2017-02-14 | Ambrx, Inc. | Therapeutic uses of modified relaxin polypeptides |
MY162837A (en) | 2010-08-17 | 2017-07-31 | Ambrx Inc | Modified relaxin polypeptides and their uses |
AR083006A1 (es) | 2010-09-23 | 2013-01-23 | Lilly Co Eli | Formulaciones para el factor estimulante de colonias de granulocitos (g-csf) bovino y variantes de las mismas |
CA2839570C (en) | 2011-06-23 | 2020-04-07 | Rho Renewables, Inc. | Recombinant production systems for decarboxylated aromatic molecules |
US9783570B2 (en) | 2011-07-01 | 2017-10-10 | Hoffmann-La Roche Inc. | Method for separation of monomeric polypeptides from aggregated polypeptides |
UY36370A (es) | 2014-10-24 | 2016-04-29 | Bristol Myers Squibb Company Una Corporación Del Estado De Delaware | Polipéptidos fgf-21 modificados y sus usos |
ES2963839T3 (es) | 2017-02-08 | 2024-04-02 | Bristol Myers Squibb Co | Polipéptidos de relaxina modificados que comprenden un potenciador farmacocinético y usos de los mismos |
WO2022097216A1 (ja) * | 2020-11-05 | 2022-05-12 | シミックホールディングス株式会社 | 組み換えインスリン様成長因子iの製造方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BG49718A3 (en) * | 1983-07-15 | 1992-01-15 | Bio Technology General Corp | Method for preparing of polypeptid with superoxiddismutasne activitty |
US4963665A (en) * | 1986-01-07 | 1990-10-16 | Washington University | Human preproinsulin-like growth factor I |
US4769361A (en) * | 1986-08-07 | 1988-09-06 | International Minerals & Chemical Corp. | Method for purifying and isolating two ionic forms of somatomedin C |
GB8819826D0 (en) * | 1988-08-20 | 1988-09-21 | Kabivitrum Ab | Glycosylated igf-1 |
-
1991
- 1991-10-28 US US07/785,171 patent/US5231178A/en not_active Expired - Lifetime
-
1992
- 1992-01-15 EP EP92904088A patent/EP0567554B1/de not_active Expired - Lifetime
- 1992-01-15 AU AU11947/92A patent/AU1194792A/en not_active Abandoned
- 1992-01-15 ES ES92904088T patent/ES2071488T3/es not_active Expired - Lifetime
- 1992-01-15 WO PCT/US1992/000261 patent/WO1992012993A1/en active IP Right Grant
- 1992-01-15 CA CA002098183A patent/CA2098183C/en not_active Expired - Fee Related
- 1992-01-15 JP JP4504533A patent/JPH06505010A/ja active Pending
- 1992-01-15 AT AT92904088T patent/ATE121099T1/de not_active IP Right Cessation
- 1992-01-15 DE DE69202049T patent/DE69202049T2/de not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
AU1194792A (en) | 1992-08-27 |
CA2098183C (en) | 2000-09-05 |
DE69202049D1 (de) | 1995-05-18 |
CA2098183A1 (en) | 1992-07-16 |
DE69202049T2 (de) | 1995-08-24 |
US5231178A (en) | 1993-07-27 |
EP0567554B1 (de) | 1995-04-12 |
WO1992012993A1 (en) | 1992-08-06 |
ES2071488T3 (es) | 1995-06-16 |
JPH06505010A (ja) | 1994-06-09 |
EP0567554A1 (de) | 1993-11-03 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |