AR102420A1 - Sitios de integración en celulas cho y sus usos - Google Patents
Sitios de integración en celulas cho y sus usosInfo
- Publication number
- AR102420A1 AR102420A1 ARP150103445A ARP150103445A AR102420A1 AR 102420 A1 AR102420 A1 AR 102420A1 AR P150103445 A ARP150103445 A AR P150103445A AR P150103445 A ARP150103445 A AR P150103445A AR 102420 A1 AR102420 A1 AR 102420A1
- Authority
- AR
- Argentina
- Prior art keywords
- expression
- integration sites
- cho cells
- eukaryotic
- improved
- Prior art date
Links
- 230000010354 integration Effects 0.000 title abstract 2
- 230000014509 gene expression Effects 0.000 abstract 4
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 230000010473 stable expression Effects 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 230000002759 chromosomal effect Effects 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462067774P | 2014-10-23 | 2014-10-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR102420A1 true AR102420A1 (es) | 2017-03-01 |
Family
ID=54602002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP150103445A AR102420A1 (es) | 2014-10-23 | 2015-10-23 | Sitios de integración en celulas cho y sus usos |
Country Status (16)
| Country | Link |
|---|---|
| US (5) | US9816110B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP3209785B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6668340B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR102243243B1 (cg-RX-API-DMAC7.html) |
| CN (1) | CN107109434A (cg-RX-API-DMAC7.html) |
| AR (1) | AR102420A1 (cg-RX-API-DMAC7.html) |
| AU (1) | AU2015335921B2 (cg-RX-API-DMAC7.html) |
| BR (1) | BR112017008022A2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2965495C (cg-RX-API-DMAC7.html) |
| EA (1) | EA037255B1 (cg-RX-API-DMAC7.html) |
| IL (1) | IL251674B (cg-RX-API-DMAC7.html) |
| MX (1) | MX2017005306A (cg-RX-API-DMAC7.html) |
| MY (1) | MY184103A (cg-RX-API-DMAC7.html) |
| SG (1) | SG11201703149RA (cg-RX-API-DMAC7.html) |
| TW (2) | TWI747808B (cg-RX-API-DMAC7.html) |
| WO (1) | WO2016064999A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG11201703149RA (en) | 2014-10-23 | 2018-02-27 | Regeneron Pharma | Novel cho integration sites and uses thereof |
| US10329594B1 (en) | 2015-09-03 | 2019-06-25 | CHO Plus, Inc. | Cell lines for high level production of protein-based pharmaceuticals |
| US11649449B2 (en) | 2015-09-03 | 2023-05-16 | CHO Plus, Inc. | Hybrid cell lines for high level production of a target protein |
| SG10202010156XA (en) | 2016-04-20 | 2020-11-27 | Regeneron Pharma | Compositions and methods for making antibodies based on use of an expression-enhancing locus |
| EP3445781A1 (en) | 2016-04-20 | 2019-02-27 | Regeneron Pharmaceuticals, Inc. | Compositions and methods for making antibodies based on use of expression-enhancing loci |
| EP3504328A1 (en) | 2016-08-24 | 2019-07-03 | Regeneron Pharmaceuticals, Inc. | Host cell protein modification |
| JP2020501593A (ja) * | 2016-12-20 | 2020-01-23 | ディベロップメント センター フォー バイオテクノロジーＤｅｖｅｌｏｐｍｅｎｔ Ｃｅｎｔｅｒ Ｆｏｒ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ | 改変細胞、調製方法、及び構築物 |
| WO2018148196A1 (en) * | 2017-02-07 | 2018-08-16 | Sigma-Aldrich Co. Llc | Stable targeted integration |
| JP7637470B2 (ja) * | 2017-02-17 | 2025-02-28 | ロンザ リミテッド | アデノ随伴ウイルスを生産するための哺乳動物細胞 |
| WO2018175153A1 (en) * | 2017-03-19 | 2018-09-27 | Applied Stemcell, Inc. | Novel integration sites and uses thereof |
| AU2018315428B2 (en) | 2017-08-11 | 2024-11-14 | Boehringer Ingelheim International Gmbh | Integration sites in CHO cells |
| CN107723276B (zh) * | 2017-11-02 | 2021-08-13 | 上海交通大学 | 一种稳定高表达目标产物的细胞株的构建方法和试剂盒 |
| US20200318106A1 (en) * | 2017-12-22 | 2020-10-08 | Bio-Rad Laboratories, Inc. | Controlling phenotype of organisms with crispr/cas gene targeting |
| IL275462B1 (en) * | 2017-12-22 | 2025-09-01 | Genentech Inc | Targeted integration of nucleic acids |
| CN109295092B (zh) * | 2018-10-30 | 2021-06-29 | 江南大学 | 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用 |
| CN109337927B (zh) * | 2018-10-30 | 2021-07-06 | 江南大学 | 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用 |
| CN109295093B (zh) * | 2018-10-30 | 2021-08-03 | 江南大学 | 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用 |
| CN109207432B (zh) * | 2018-10-30 | 2021-08-03 | 江南大学 | 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用 |
| CN109136193B (zh) * | 2018-10-30 | 2021-07-06 | 江南大学 | 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用 |
| CN109321604B (zh) * | 2018-10-30 | 2021-07-06 | 江南大学 | 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用 |
| AU2019403279A1 (en) * | 2018-12-21 | 2021-07-01 | Genentech, Inc. | Targeted integration of nucleic acids |
| SG11202110076PA (en) | 2019-04-02 | 2021-10-28 | Chugai Pharmaceutical Co Ltd | Method of introducing target-specific foreign gene |
| EP3990649A1 (en) | 2019-06-26 | 2022-05-04 | Genentech, Inc. | Randomized configuration targeted integration of nucleic acids |
| EP3901266A1 (en) * | 2020-04-22 | 2021-10-27 | LEK Pharmaceuticals d.d. | Super-enhancers for recombinant gene expression in cho cells |
| CN116134136A (zh) * | 2020-06-02 | 2023-05-16 | 康泰伦特药物解决方案有限责任公司 | 具有多个用于基因插入的Dock的细胞系 |
| CA3172453A1 (en) * | 2020-06-24 | 2021-12-30 | Amy Shen | Targeted integration of nucleic acids |
| EP4229205B1 (en) | 2020-10-15 | 2025-05-14 | F. Hoffmann-La Roche AG | Nucleic acid constructs for va rna transcription |
| CN113755447B (zh) * | 2021-09-23 | 2022-04-29 | 云舟生物科技(广州)股份有限公司 | 一种用于生产腺病毒的293a细胞株及其制备与应用 |
| US20230287460A1 (en) | 2021-10-18 | 2023-09-14 | Regeneron Pharmaceuticals, Inc. | Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites |
| KR20240099288A (ko) | 2021-10-18 | 2024-06-28 | 리제너론 파마슈티칼스 인코포레이티드 | 아데노바이러스-연관 바이러스 폴리뉴클레오티드를 포함하는 진핵세포 |
| TW202330926A (zh) | 2021-10-18 | 2023-08-01 | 美商再生元醫藥公司 | 聚核苷酸之受控制轉錄 |
| JP2025503925A (ja) * | 2022-01-28 | 2025-02-06 | ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク | 異種移植のための遺伝子改変 |
| CN117305334B (zh) * | 2022-06-21 | 2024-11-08 | 深圳太力生物技术有限责任公司 | 靶向整合细胞及其制备方法、生产目标基因表达产物的方法 |
| TW202434888A (zh) | 2022-11-18 | 2024-09-01 | 美商再生元醫藥公司 | 用於偵測及測定包括生物流體在內之流體中之蛋白質結構及穩定性的方法 |
| CN118726475B (zh) * | 2023-03-28 | 2025-07-08 | 深圳太力生物技术有限责任公司 | 核酸、重组载体、靶向整合细胞、基因表达方法和应用 |
| WO2024211287A1 (en) * | 2023-04-03 | 2024-10-10 | Seagen Inc. | Production cell lines with targeted integration sites |
| US20250122482A1 (en) | 2023-09-07 | 2025-04-17 | Regeneron Pharmaceuticals, Inc. | Production and purification of covalently surface modified adeno-associated virus |
| WO2025111473A1 (en) | 2023-11-21 | 2025-05-30 | Regeneron Pharmaceuticals, Inc. | Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4634665A (en) | 1980-02-25 | 1987-01-06 | The Trustees Of Columbia University In The City Of New York | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
| US4656134A (en) | 1982-01-11 | 1987-04-07 | Board Of Trustees Of Leland Stanford Jr. University | Gene amplification in eukaryotic cells |
| ATE539161T1 (de) | 1998-07-21 | 2012-01-15 | Millipore Corp | Ein ein allgegenwärtiges chromatin-öffnendes element (ucoe) enthaltendes polynucleotid |
| US6521419B1 (en) * | 1998-09-22 | 2003-02-18 | Kanakaraju Koduri | Expression vectors containing hot spot for increased recombinant protein expression in transfected cells |
| WO2002081632A2 (en) | 2001-04-04 | 2002-10-17 | The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services | Nucleic acids for transgene expression |
| US20040045043A1 (en) * | 2002-05-20 | 2004-03-04 | Finney Robert E. | Compositions and methods for generating conditional knockouts |
| US8673589B2 (en) | 2002-05-29 | 2014-03-18 | Regeneron Pharmaceuticals, Inc. | Inducible eukaryotic expression system |
| EP2150617B1 (en) | 2007-06-04 | 2014-10-22 | Regeneron Pharmaceuticals, Inc. | Enhanced expression and stability regions |
| CA2745031C (en) * | 2008-12-04 | 2018-08-14 | Sangamo Biosciences, Inc. | Genome editing in rats using zinc-finger nucleases |
| CN105969807A (zh) | 2011-04-05 | 2016-09-28 | 斯克利普斯研究所 | 染色体着陆垫及相关用途 |
| EP3403672A1 (en) | 2011-04-20 | 2018-11-21 | Medlmmune, LLC | Antibodies and other molecules that bind b7-h1 and pd-1 |
| WO2012167192A2 (en) * | 2011-06-01 | 2012-12-06 | Precision Biosciences, Inc. | Methods and products for producing engineered mammalian cell lines with amplified transgenes |
| AU2012328682B2 (en) * | 2011-10-27 | 2017-09-21 | Sangamo Therapeutics, Inc. | Methods and compositions for modification of the HPRT locus |
| AU2013259647B2 (en) | 2012-05-07 | 2018-11-08 | Corteva Agriscience Llc | Methods and compositions for nuclease-mediated targeted integration of transgenes |
| SG11201703149RA (en) | 2014-10-23 | 2018-02-27 | Regeneron Pharma | Novel cho integration sites and uses thereof |
-
2015
- 2015-10-21 SG SG11201703149RA patent/SG11201703149RA/en unknown
- 2015-10-21 MY MYPI2017701254A patent/MY184103A/en unknown
- 2015-10-21 CN CN201580069889.1A patent/CN107109434A/zh active Pending
- 2015-10-21 KR KR1020177012465A patent/KR102243243B1/ko active Active
- 2015-10-21 EP EP15797495.7A patent/EP3209785B1/en active Active
- 2015-10-21 EA EA201790698A patent/EA037255B1/ru unknown
- 2015-10-21 US US14/919,300 patent/US9816110B2/en active Active
- 2015-10-21 MX MX2017005306A patent/MX2017005306A/es unknown
- 2015-10-21 WO PCT/US2015/056653 patent/WO2016064999A1/en not_active Ceased
- 2015-10-21 BR BR112017008022-2A patent/BR112017008022A2/pt not_active Application Discontinuation
- 2015-10-21 CA CA2965495A patent/CA2965495C/en active Active
- 2015-10-21 AU AU2015335921A patent/AU2015335921B2/en active Active
- 2015-10-21 JP JP2017519646A patent/JP6668340B2/ja active Active
- 2015-10-22 TW TW104134716A patent/TWI747808B/zh active
- 2015-10-22 TW TW110128495A patent/TWI780847B/zh active
- 2015-10-23 AR ARP150103445A patent/AR102420A1/es unknown
-
2017
- 2017-04-09 IL IL251674A patent/IL251674B/en active IP Right Grant
- 2017-10-13 US US15/783,525 patent/US20180030480A1/en not_active Abandoned
-
2019
- 2019-09-19 US US16/575,506 patent/US11788102B2/en active Active
-
2021
- 2021-02-12 US US17/175,025 patent/US11268109B2/en active Active
-
2023
- 2023-09-06 US US18/461,798 patent/US20240018553A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR102243243B1 (ko) | 2021-04-22 |
| AU2015335921A1 (en) | 2017-05-04 |
| EA201790698A1 (ru) | 2018-01-31 |
| EA037255B1 (ru) | 2021-02-26 |
| BR112017008022A2 (pt) | 2018-02-20 |
| US20240018553A1 (en) | 2024-01-18 |
| US11268109B2 (en) | 2022-03-08 |
| US11788102B2 (en) | 2023-10-17 |
| US20160115502A1 (en) | 2016-04-28 |
| WO2016064999A1 (en) | 2016-04-28 |
| JP6668340B2 (ja) | 2020-03-18 |
| US20210171984A1 (en) | 2021-06-10 |
| KR20170096999A (ko) | 2017-08-25 |
| CA2965495A1 (en) | 2016-04-28 |
| TW201629229A (zh) | 2016-08-16 |
| EP3209785A1 (en) | 2017-08-30 |
| IL251674B (en) | 2020-10-29 |
| TW202144576A (zh) | 2021-12-01 |
| MX2017005306A (es) | 2018-01-09 |
| EP3209785B1 (en) | 2019-07-31 |
| CN107109434A (zh) | 2017-08-29 |
| US20200002732A1 (en) | 2020-01-02 |
| US20180030480A1 (en) | 2018-02-01 |
| CA2965495C (en) | 2022-05-24 |
| TWI747808B (zh) | 2021-12-01 |
| JP2017535258A (ja) | 2017-11-30 |
| US9816110B2 (en) | 2017-11-14 |
| SG11201703149RA (en) | 2018-02-27 |
| IL251674A0 (en) | 2017-06-29 |
| MY184103A (en) | 2021-03-18 |
| TWI780847B (zh) | 2022-10-11 |
| AU2015335921B2 (en) | 2020-06-25 |
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