EA037255B1 - Новые сайты интеграции cho и их применения - Google Patents

Новые сайты интеграции cho и их применения Download PDF

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Publication number
EA037255B1
EA037255B1 EA201790698A EA201790698A EA037255B1 EA 037255 B1 EA037255 B1 EA 037255B1 EA 201790698 A EA201790698 A EA 201790698A EA 201790698 A EA201790698 A EA 201790698A EA 037255 B1 EA037255 B1 EA 037255B1
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EA
Eurasian Patent Office
Prior art keywords
exogenous
site
goi
nucleic acid
seq
Prior art date
Application number
EA201790698A
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English (en)
Russian (ru)
Other versions
EA201790698A1 (ru
Inventor
Ин Шень
Дарья Бураков
Ган Чень
Джэймс П. Фендл
Original Assignee
Регенерон Фармасьютикалз, Инк.
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=54602002&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA037255(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Регенерон Фармасьютикалз, Инк. filed Critical Регенерон Фармасьютикалз, Инк.
Publication of EA201790698A1 publication Critical patent/EA201790698A1/ru
Publication of EA037255B1 publication Critical patent/EA037255B1/ru

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
EA201790698A 2014-10-23 2015-10-21 Новые сайты интеграции cho и их применения EA037255B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23
PCT/US2015/056653 WO2016064999A1 (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

Publications (2)

Publication Number Publication Date
EA201790698A1 EA201790698A1 (ru) 2018-01-31
EA037255B1 true EA037255B1 (ru) 2021-02-26

Family

ID=54602002

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201790698A EA037255B1 (ru) 2014-10-23 2015-10-21 Новые сайты интеграции cho и их применения

Country Status (16)

Country Link
US (5) US9816110B2 (cg-RX-API-DMAC7.html)
EP (1) EP3209785B1 (cg-RX-API-DMAC7.html)
JP (1) JP6668340B2 (cg-RX-API-DMAC7.html)
KR (1) KR102243243B1 (cg-RX-API-DMAC7.html)
CN (1) CN107109434A (cg-RX-API-DMAC7.html)
AR (1) AR102420A1 (cg-RX-API-DMAC7.html)
AU (1) AU2015335921B2 (cg-RX-API-DMAC7.html)
BR (1) BR112017008022A2 (cg-RX-API-DMAC7.html)
CA (1) CA2965495C (cg-RX-API-DMAC7.html)
EA (1) EA037255B1 (cg-RX-API-DMAC7.html)
IL (1) IL251674B (cg-RX-API-DMAC7.html)
MX (1) MX2017005306A (cg-RX-API-DMAC7.html)
MY (1) MY184103A (cg-RX-API-DMAC7.html)
SG (1) SG11201703149RA (cg-RX-API-DMAC7.html)
TW (2) TWI747808B (cg-RX-API-DMAC7.html)
WO (1) WO2016064999A1 (cg-RX-API-DMAC7.html)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201703149RA (en) 2014-10-23 2018-02-27 Regeneron Pharma Novel cho integration sites and uses thereof
US10329594B1 (en) 2015-09-03 2019-06-25 CHO Plus, Inc. Cell lines for high level production of protein-based pharmaceuticals
US11649449B2 (en) 2015-09-03 2023-05-16 CHO Plus, Inc. Hybrid cell lines for high level production of a target protein
SG10202010156XA (en) 2016-04-20 2020-11-27 Regeneron Pharma Compositions and methods for making antibodies based on use of an expression-enhancing locus
EP3445781A1 (en) 2016-04-20 2019-02-27 Regeneron Pharmaceuticals, Inc. Compositions and methods for making antibodies based on use of expression-enhancing loci
EP3504328A1 (en) 2016-08-24 2019-07-03 Regeneron Pharmaceuticals, Inc. Host cell protein modification
JP2020501593A (ja) * 2016-12-20 2020-01-23 ディベロップメント センター フォー バイオテクノロジーDevelopment Center For Biotechnology 改変細胞、調製方法、及び構築物
WO2018148196A1 (en) * 2017-02-07 2018-08-16 Sigma-Aldrich Co. Llc Stable targeted integration
JP7637470B2 (ja) * 2017-02-17 2025-02-28 ロンザ リミテッド アデノ随伴ウイルスを生産するための哺乳動物細胞
WO2018175153A1 (en) * 2017-03-19 2018-09-27 Applied Stemcell, Inc. Novel integration sites and uses thereof
AU2018315428B2 (en) 2017-08-11 2024-11-14 Boehringer Ingelheim International Gmbh Integration sites in CHO cells
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
US20200318106A1 (en) * 2017-12-22 2020-10-08 Bio-Rad Laboratories, Inc. Controlling phenotype of organisms with crispr/cas gene targeting
IL275462B1 (en) * 2017-12-22 2025-09-01 Genentech Inc Targeted integration of nucleic acids
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
AU2019403279A1 (en) * 2018-12-21 2021-07-01 Genentech, Inc. Targeted integration of nucleic acids
SG11202110076PA (en) 2019-04-02 2021-10-28 Chugai Pharmaceutical Co Ltd Method of introducing target-specific foreign gene
EP3990649A1 (en) 2019-06-26 2022-05-04 Genentech, Inc. Randomized configuration targeted integration of nucleic acids
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
CN116134136A (zh) * 2020-06-02 2023-05-16 康泰伦特药物解决方案有限责任公司 具有多个用于基因插入的Dock的细胞系
CA3172453A1 (en) * 2020-06-24 2021-12-30 Amy Shen Targeted integration of nucleic acids
EP4229205B1 (en) 2020-10-15 2025-05-14 F. Hoffmann-La Roche AG Nucleic acid constructs for va rna transcription
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
US20230287460A1 (en) 2021-10-18 2023-09-14 Regeneron Pharmaceuticals, Inc. Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites
KR20240099288A (ko) 2021-10-18 2024-06-28 리제너론 파마슈티칼스 인코포레이티드 아데노바이러스-연관 바이러스 폴리뉴클레오티드를 포함하는 진핵세포
TW202330926A (zh) 2021-10-18 2023-08-01 美商再生元醫藥公司 聚核苷酸之受控制轉錄
JP2025503925A (ja) * 2022-01-28 2025-02-06 ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク 異種移植のための遺伝子改変
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
TW202434888A (zh) 2022-11-18 2024-09-01 美商再生元醫藥公司 用於偵測及測定包括生物流體在內之流體中之蛋白質結構及穩定性的方法
CN118726475B (zh) * 2023-03-28 2025-07-08 深圳太力生物技术有限责任公司 核酸、重组载体、靶向整合细胞、基因表达方法和应用
WO2024211287A1 (en) * 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
US20250122482A1 (en) 2023-09-07 2025-04-17 Regeneron Pharmaceuticals, Inc. Production and purification of covalently surface modified adeno-associated virus
WO2025111473A1 (en) 2023-11-21 2025-05-30 Regeneron Pharmaceuticals, Inc. Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008151219A1 (en) * 2007-06-04 2008-12-11 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
WO2012138887A1 (en) * 2011-04-05 2012-10-11 The Scripps Research Institute Chromosomal landing pads and related uses

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
ATE539161T1 (de) 1998-07-21 2012-01-15 Millipore Corp Ein ein allgegenwärtiges chromatin-öffnendes element (ucoe) enthaltendes polynucleotid
US6521419B1 (en) * 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
WO2002081632A2 (en) 2001-04-04 2002-10-17 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services Nucleic acids for transgene expression
US20040045043A1 (en) * 2002-05-20 2004-03-04 Finney Robert E. Compositions and methods for generating conditional knockouts
US8673589B2 (en) 2002-05-29 2014-03-18 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
CA2745031C (en) * 2008-12-04 2018-08-14 Sangamo Biosciences, Inc. Genome editing in rats using zinc-finger nucleases
EP3403672A1 (en) 2011-04-20 2018-11-21 Medlmmune, LLC Antibodies and other molecules that bind b7-h1 and pd-1
WO2012167192A2 (en) * 2011-06-01 2012-12-06 Precision Biosciences, Inc. Methods and products for producing engineered mammalian cell lines with amplified transgenes
AU2012328682B2 (en) * 2011-10-27 2017-09-21 Sangamo Therapeutics, Inc. Methods and compositions for modification of the HPRT locus
AU2013259647B2 (en) 2012-05-07 2018-11-08 Corteva Agriscience Llc Methods and compositions for nuclease-mediated targeted integration of transgenes
SG11201703149RA (en) 2014-10-23 2018-02-27 Regeneron Pharma Novel cho integration sites and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008151219A1 (en) * 2007-06-04 2008-12-11 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
WO2012138887A1 (en) * 2011-04-05 2012-10-11 The Scripps Research Institute Chromosomal landing pads and related uses

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHRISTINE LATTENMAYER ; MARTINA LOESCHEL ; WILLIBALD STEINFELLNER ; EVELYN TRUMMER ; DETHARDT MUELLER ; KORNELIA SCHRIEBL ; KAROLA: "Identification of transgene integration loci of different highly expressing recombinant CHO cell lines by FISH", CYTOTECHNOLOGY, KLUWER ACADEMIC PUBLISHERS, DO, vol. 51, no. 3, 15 November 2006 (2006-11-15), Do, pages 171 - 182, XP019448503, ISSN: 1573-0778, DOI: 10.1007/s10616-006-9029-0 *
HONG ZHOU, ZHI-GANG LIU, ZHI-WEI SUN, YING HUANG, WEI-YUAN YU: "Generation of stable cell lines by site-specific integration of transgenes into engineered Chinese hamster ovary strains using an FLP-FRT system.", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 147, no. 2, 17 May 2010 (2010-05-17), Amsterdam NL, pages 122 - 129, XP002711613, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2010.03.020 *
LEWIS N E, LIU X, LI Y, NAGARAJAN H, YERGANIAN G, O'BRIEN E, BORDBAR A, ROTH A M, ROSENBLOOM J, BIAN C, XIE M, CHEN W, LI N, BAYCI: "Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome.", NATURE BIOTECHNOLOGY, vol. 31, no. 8, 1 August 2013 (2013-08-01), pages 759 - 765, XP002753785, ISSN: 1546-1696, DOI: 10.1038/nbt.2624 *
MICHEL SADELAIN, EIRINI P. PAPAPETROU, FREDERIC D. BUSHMAN: "Safe harbours for the integration of new DNA in the human genome", NATURE REVIEWS CANCER, NATURE PUB. GROUP, vol. 12, no. 1, 1 January 2011 (2011-01-01), pages 51 - 58, XP055018235, ISSN: 1474175X, DOI: 10.1038/nrc3179 *
SCOTT BAHR;LAURA CORTNER;SARA LADLEY;TRISSA BORGSCHULTE: "Evaluating the effect of chromosomal context on zinc finger nuclease efficiency", BMC PROCEEDINGS, BIOMED CENTRAL LTD, LONDON UK, vol. 7, no. Suppl 6, 4 December 2013 (2013-12-04), London UK, pages P3, XP021170326, ISSN: 1753-6561, DOI: 10.1186/1753-6561-7-S6-P3 *
XUN XU, HARISH NAGARAJAN, NATHAN E LEWIS, SHENGKAI PAN, ZHIMING CAI, XIN LIU, WENBIN CHEN, MIN XIE, WENLIANG WANG, STEPHANIE HAMMO: "The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line", NATURE BIOTECHNOLOGY, GALE GROUP INC., NEW YORK, US, vol. 29, no. 8, us, pages 735 - 741, XP055223996, ISSN: 1087-0156, DOI: 10.1038/nbt.1932 *

Also Published As

Publication number Publication date
KR102243243B1 (ko) 2021-04-22
AU2015335921A1 (en) 2017-05-04
EA201790698A1 (ru) 2018-01-31
BR112017008022A2 (pt) 2018-02-20
US20240018553A1 (en) 2024-01-18
US11268109B2 (en) 2022-03-08
US11788102B2 (en) 2023-10-17
US20160115502A1 (en) 2016-04-28
WO2016064999A1 (en) 2016-04-28
JP6668340B2 (ja) 2020-03-18
US20210171984A1 (en) 2021-06-10
AR102420A1 (es) 2017-03-01
KR20170096999A (ko) 2017-08-25
CA2965495A1 (en) 2016-04-28
TW201629229A (zh) 2016-08-16
EP3209785A1 (en) 2017-08-30
IL251674B (en) 2020-10-29
TW202144576A (zh) 2021-12-01
MX2017005306A (es) 2018-01-09
EP3209785B1 (en) 2019-07-31
CN107109434A (zh) 2017-08-29
US20200002732A1 (en) 2020-01-02
US20180030480A1 (en) 2018-02-01
CA2965495C (en) 2022-05-24
TWI747808B (zh) 2021-12-01
JP2017535258A (ja) 2017-11-30
US9816110B2 (en) 2017-11-14
SG11201703149RA (en) 2018-02-27
IL251674A0 (en) 2017-06-29
MY184103A (en) 2021-03-18
TWI780847B (zh) 2022-10-11
AU2015335921B2 (en) 2020-06-25

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