AU2015335921B2 - Novel CHO integration sites and uses thereof - Google Patents

Novel CHO integration sites and uses thereof Download PDF

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Publication number
AU2015335921B2
AU2015335921B2 AU2015335921A AU2015335921A AU2015335921B2 AU 2015335921 B2 AU2015335921 B2 AU 2015335921B2 AU 2015335921 A AU2015335921 A AU 2015335921A AU 2015335921 A AU2015335921 A AU 2015335921A AU 2015335921 B2 AU2015335921 B2 AU 2015335921B2
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site
exogenous
goi
nucleic acid
seq
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AU2015335921A1 (en
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Darya Burakov
Gang Chen
James P. Fandl
Ying Shen
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
AU2015335921A 2014-10-23 2015-10-21 Novel CHO integration sites and uses thereof Active AU2015335921B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23
US62/067,774 2014-10-23
PCT/US2015/056653 WO2016064999A1 (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

Publications (2)

Publication Number Publication Date
AU2015335921A1 AU2015335921A1 (en) 2017-05-04
AU2015335921B2 true AU2015335921B2 (en) 2020-06-25

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AU2015335921A Active AU2015335921B2 (en) 2014-10-23 2015-10-21 Novel CHO integration sites and uses thereof

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US (5) US9816110B2 (cg-RX-API-DMAC7.html)
EP (1) EP3209785B1 (cg-RX-API-DMAC7.html)
JP (1) JP6668340B2 (cg-RX-API-DMAC7.html)
KR (1) KR102243243B1 (cg-RX-API-DMAC7.html)
CN (1) CN107109434A (cg-RX-API-DMAC7.html)
AR (1) AR102420A1 (cg-RX-API-DMAC7.html)
AU (1) AU2015335921B2 (cg-RX-API-DMAC7.html)
BR (1) BR112017008022A2 (cg-RX-API-DMAC7.html)
CA (1) CA2965495C (cg-RX-API-DMAC7.html)
EA (1) EA037255B1 (cg-RX-API-DMAC7.html)
IL (1) IL251674B (cg-RX-API-DMAC7.html)
MX (1) MX2017005306A (cg-RX-API-DMAC7.html)
MY (1) MY184103A (cg-RX-API-DMAC7.html)
SG (1) SG11201703149RA (cg-RX-API-DMAC7.html)
TW (2) TWI747808B (cg-RX-API-DMAC7.html)
WO (1) WO2016064999A1 (cg-RX-API-DMAC7.html)

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WO2018148196A1 (en) * 2017-02-07 2018-08-16 Sigma-Aldrich Co. Llc Stable targeted integration
JP7637470B2 (ja) * 2017-02-17 2025-02-28 ロンザ リミテッド アデノ随伴ウイルスを生産するための哺乳動物細胞
WO2018175153A1 (en) * 2017-03-19 2018-09-27 Applied Stemcell, Inc. Novel integration sites and uses thereof
AU2018315428B2 (en) 2017-08-11 2024-11-14 Boehringer Ingelheim International Gmbh Integration sites in CHO cells
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
US20200318106A1 (en) * 2017-12-22 2020-10-08 Bio-Rad Laboratories, Inc. Controlling phenotype of organisms with crispr/cas gene targeting
IL275462B1 (en) * 2017-12-22 2025-09-01 Genentech Inc Targeted integration of nucleic acids
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
AU2019403279A1 (en) * 2018-12-21 2021-07-01 Genentech, Inc. Targeted integration of nucleic acids
SG11202110076PA (en) 2019-04-02 2021-10-28 Chugai Pharmaceutical Co Ltd Method of introducing target-specific foreign gene
EP3990649A1 (en) 2019-06-26 2022-05-04 Genentech, Inc. Randomized configuration targeted integration of nucleic acids
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
CN116134136A (zh) * 2020-06-02 2023-05-16 康泰伦特药物解决方案有限责任公司 具有多个用于基因插入的Dock的细胞系
CA3172453A1 (en) * 2020-06-24 2021-12-30 Amy Shen Targeted integration of nucleic acids
EP4229205B1 (en) 2020-10-15 2025-05-14 F. Hoffmann-La Roche AG Nucleic acid constructs for va rna transcription
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
US20230287460A1 (en) 2021-10-18 2023-09-14 Regeneron Pharmaceuticals, Inc. Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites
KR20240099288A (ko) 2021-10-18 2024-06-28 리제너론 파마슈티칼스 인코포레이티드 아데노바이러스-연관 바이러스 폴리뉴클레오티드를 포함하는 진핵세포
TW202330926A (zh) 2021-10-18 2023-08-01 美商再生元醫藥公司 聚核苷酸之受控制轉錄
JP2025503925A (ja) * 2022-01-28 2025-02-06 ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク 異種移植のための遺伝子改変
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
TW202434888A (zh) 2022-11-18 2024-09-01 美商再生元醫藥公司 用於偵測及測定包括生物流體在內之流體中之蛋白質結構及穩定性的方法
CN118726475B (zh) * 2023-03-28 2025-07-08 深圳太力生物技术有限责任公司 核酸、重组载体、靶向整合细胞、基因表达方法和应用
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WO2025111473A1 (en) 2023-11-21 2025-05-30 Regeneron Pharmaceuticals, Inc. Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus

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Also Published As

Publication number Publication date
KR102243243B1 (ko) 2021-04-22
AU2015335921A1 (en) 2017-05-04
EA201790698A1 (ru) 2018-01-31
EA037255B1 (ru) 2021-02-26
BR112017008022A2 (pt) 2018-02-20
US20240018553A1 (en) 2024-01-18
US11268109B2 (en) 2022-03-08
US11788102B2 (en) 2023-10-17
US20160115502A1 (en) 2016-04-28
WO2016064999A1 (en) 2016-04-28
JP6668340B2 (ja) 2020-03-18
US20210171984A1 (en) 2021-06-10
AR102420A1 (es) 2017-03-01
KR20170096999A (ko) 2017-08-25
CA2965495A1 (en) 2016-04-28
TW201629229A (zh) 2016-08-16
EP3209785A1 (en) 2017-08-30
IL251674B (en) 2020-10-29
TW202144576A (zh) 2021-12-01
MX2017005306A (es) 2018-01-09
EP3209785B1 (en) 2019-07-31
CN107109434A (zh) 2017-08-29
US20200002732A1 (en) 2020-01-02
US20180030480A1 (en) 2018-02-01
CA2965495C (en) 2022-05-24
TWI747808B (zh) 2021-12-01
JP2017535258A (ja) 2017-11-30
US9816110B2 (en) 2017-11-14
SG11201703149RA (en) 2018-02-27
IL251674A0 (en) 2017-06-29
MY184103A (en) 2021-03-18
TWI780847B (zh) 2022-10-11

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