AR092616A1 - Vectores y metodos para mejorar la expresion de proteinas recombinantes en plantas - Google Patents
Vectores y metodos para mejorar la expresion de proteinas recombinantes en plantasInfo
- Publication number
- AR092616A1 AR092616A1 ARP130103350A ARP130103350A AR092616A1 AR 092616 A1 AR092616 A1 AR 092616A1 AR P130103350 A ARP130103350 A AR P130103350A AR P130103350 A ARP130103350 A AR P130103350A AR 092616 A1 AR092616 A1 AR 092616A1
- Authority
- AR
- Argentina
- Prior art keywords
- utr
- terminator sequence
- gene
- rbc
- morifolium
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 239000013598 vector Substances 0.000 title 1
- 108091023045 Untranslated Region Proteins 0.000 abstract 8
- 108700007698 Genetic Terminator Regions Proteins 0.000 abstract 5
- 244000189548 Chrysanthemum x morifolium Species 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 241000701489 Cauliflower mosaic virus Species 0.000 abstract 3
- 239000013604 expression vector Substances 0.000 abstract 3
- 241000196324 Embryophyta Species 0.000 abstract 2
- 241000589158 Agrobacterium Species 0.000 abstract 1
- 235000009604 Chrysanthemum X morifolium Nutrition 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 abstract 1
- 241000207746 Nicotiana benthamiana Species 0.000 abstract 1
- 244000061176 Nicotiana tabacum Species 0.000 abstract 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 abstract 1
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 235000007164 Oryza sativa Nutrition 0.000 abstract 1
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 abstract 1
- 240000003768 Solanum lycopersicum Species 0.000 abstract 1
- 241000700605 Viruses Species 0.000 abstract 1
- 101710136524 X polypeptide Proteins 0.000 abstract 1
- 229960000397 bevacizumab Drugs 0.000 abstract 1
- 230000004186 co-expression Effects 0.000 abstract 1
- 230000030279 gene silencing Effects 0.000 abstract 1
- 238000012226 gene silencing method Methods 0.000 abstract 1
- 108010058731 nopaline synthase Proteins 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
- 229960000575 trastuzumab Drugs 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
- C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Virology (AREA)
- Immunology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Se describen vectores de expresión y métodos de uso de los mismos para mejorar la producción de proteínas recombinantes en plantas o células de las plantas. La producción puede mejorarse en forma adicional ante la co-expresión de la proteína P19 que suprime el silenciamiento génico del virus del enanismo ramificado del tomate. Preferentemente, las proteínas recombinantes son enzimas y/o anticuerpos terapéuticos, y los métodos se llevan a cabo en Nicotiana benthamiana -opcionalmente, una cepa glicomodificada sobre la base de ARNi- o en Nicotiana tabacum, cultivar Little Crittenden. Reivindicación 1: Un vector de expresión caracterizado porque comprende: (a) un promotor seleccionado entre (i) el promotor 35S del virus del mosaico de la coliflor (CaMV) o (ii) el promotor del gen de subunidad pequeña ribulosa bisfosfato carboxilasa (rbc) de Chrysanthemum morifolium; (b) una región no traducida (UTR) 5 seleccionada entre (i) la UTR 5 de 35S de CaMV o (ii) la UTR 5 del gen de subunidad pequeña rbc de C. morifolium; y (c) una UTR 3 y secuencia terminadora seleccionada entre (i) la UTR 3 y secuencia terminadora del gen nopalina sintasa (nos) de Agrobacterium, (ii) la UTR 3 y secuencia terminadora del gen osmotina (osm) de Oryza sativa, (iii) la UTR 3 y secuencia terminadora del gen de subunidad pequeña rbc de C. morifolium o (iv) una versión truncada, por 162 pb de acuerdo con lo definido por un sitio de reconocimiento de BspEl, de la UTR 3 y secuencia terminadora del gen de subunidad pequeña rbc de C. morifolium. Reivindicación 7: El vector de expresión de acuerdo con la reivindicación 6, caracterizado porque el anticuerpo es trastuzumab o bevacizumab.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261702395P | 2012-09-18 | 2012-09-18 | |
| US13/837,612 US20140090108A1 (en) | 2012-09-18 | 2013-03-15 | Vectors and Methods For Enhancing Recombinant Protein Expression in Plants |
| CA 2815847 CA2815847A1 (en) | 2012-09-18 | 2013-05-13 | Vectors and methods for enhancing recombinant protein expression in plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR092616A1 true AR092616A1 (es) | 2015-04-29 |
Family
ID=50340332
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP130103350A AR092616A1 (es) | 2012-09-18 | 2013-09-18 | Vectores y metodos para mejorar la expresion de proteinas recombinantes en plantas |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US20140090108A1 (es) |
| AR (1) | AR092616A1 (es) |
| BR (1) | BR112015005911B1 (es) |
| MX (1) | MX2015003403A (es) |
| WO (1) | WO2014043786A1 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10221402B2 (en) * | 2014-03-16 | 2019-03-05 | Plantvax, Inc. | Production of highly thermally stable recombinant cholinesterases for the detection, detoxification and decontamination of organophosphorus compounds |
| BR112017010555A2 (pt) | 2014-11-20 | 2018-02-27 | Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd | composições e métodos para produção de polipeptídeos com padrão de glicosilação modificado em células vegetais |
| EP3662067A4 (en) * | 2017-08-03 | 2021-04-14 | Plantform Corporation | TRANSIENT ATTENUATION OF ARGONAUTE1 AND ARGONAUTE4 TO INCREASE THE EXPRESSION OF RECOMBINANT PROTEINS IN PLANTS |
| CA3122080A1 (en) | 2018-12-06 | 2020-06-11 | Arcturus Therapeutics, Inc. | Compositions and methods for treating ornithine transcarbamylase deficiency |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5866787A (en) * | 1993-03-08 | 1999-02-02 | Cleveland Clinic Foundation | Transgenic plants co-expressing a functional human 2-5A system |
| US6002069A (en) * | 1996-06-05 | 1999-12-14 | The Regents Of The University Of California | Seed plants exhibiting inducible early reproductive development and methods of making same |
| US20030167531A1 (en) * | 1998-07-10 | 2003-09-04 | Russell Douglas A. | Expression and purification of bioactive, authentic polypeptides from plants |
| US9062321B2 (en) * | 2004-12-01 | 2015-06-23 | Arizona Board Of Regents On Behalf Of Arizona State University | Codon-optimized polynucleotides encoding human butyrylcholinesterase and plant expression systems therof |
| MX2007013870A (es) * | 2005-05-05 | 2008-01-24 | Monsanto Technology Llc | Plantas que contienen un gen heterologo de flavohemoglobina y sus metodos de uso. |
| ES2644883T3 (es) * | 2008-11-18 | 2017-11-30 | Commonwealth Scientific And Industrial Research Organisation | Enzimas y métodos para producir ácidos grasos omega-3 |
| PL216099B1 (pl) * | 2009-01-23 | 2014-02-28 | Inst Biotechnologii I Antybiotykow | Transgeniczne komórki roslinne produkujace bialko antygenowe HBcAg oraz ich zastosowanie do wytwarzania szczepionki |
| CA2792117C (en) * | 2010-03-12 | 2016-09-20 | Merial Limited | Foot and mouth disease virus recombinant vaccines and uses thereof |
-
2013
- 2013-03-15 US US13/837,612 patent/US20140090108A1/en not_active Abandoned
- 2013-09-17 MX MX2015003403A patent/MX2015003403A/es active IP Right Grant
- 2013-09-17 BR BR112015005911-2A patent/BR112015005911B1/pt active IP Right Grant
- 2013-09-17 WO PCT/CA2013/000780 patent/WO2014043786A1/en active Application Filing
- 2013-09-18 AR ARP130103350A patent/AR092616A1/es active IP Right Grant
-
2016
- 2016-04-26 US US15/138,626 patent/US20160289692A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20160289692A1 (en) | 2016-10-06 |
| US20140090108A1 (en) | 2014-03-27 |
| BR112015005911B1 (pt) | 2022-07-12 |
| BR112015005911A2 (pt) | 2017-08-08 |
| MX2015003403A (es) | 2015-08-12 |
| WO2014043786A1 (en) | 2014-03-27 |
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Legal Events
| Date | Code | Title | Description |
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| FG | Grant, registration |