AR086938A1 - Purificacion de anticuerpos por cromatografia de una unica unidad - Google Patents
Purificacion de anticuerpos por cromatografia de una unica unidadInfo
- Publication number
- AR086938A1 AR086938A1 ARP120102118A ARP120102118A AR086938A1 AR 086938 A1 AR086938 A1 AR 086938A1 AR P120102118 A ARP120102118 A AR P120102118A AR P120102118 A ARP120102118 A AR P120102118A AR 086938 A1 AR086938 A1 AR 086938A1
- Authority
- AR
- Argentina
- Prior art keywords
- chromatography
- anion exchange
- mixed
- exchange chromatography
- antibodies
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
Un método para purificar anticuerpos de una mezcla de proteínas que se produjo en un biorreactor, que comprende por lo menos los pasos de purificación intermedia y acabado, donde los pasos intermedio y de acabado comprenden un tratamiento en línea de cromatografía de intercambio aniónico (AEX) y un tratamiento de cromatografía de modalidad mixta (MiMo) en una modalidad de flujo directo. También una única unidad operacional que comprende tanto una parte donde se realiza una cromatografía de intercambio aniónico como una parte donde se realiza una cromatografía mixta, que están conectadas en serie, donde la unidad comprende una admisión en el extremo corriente arriba de la parte donde se realiza la cromatografía de intercambio aniónico y una salida en el extremo corriente abajo de la parte donde se realiza la cromatografía de modalidad mixta y donde la unidad también comprende una admisión entre la parte donde se realiza la cromatografía de intercambio aniónico y la parte donde se realiza la cromatografía mixta.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11170239 | 2011-06-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR086938A1 true AR086938A1 (es) | 2014-02-05 |
Family
ID=45406929
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP120102118A AR086938A1 (es) | 2011-06-16 | 2012-06-14 | Purificacion de anticuerpos por cromatografia de una unica unidad |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US20140187749A1 (es) |
| EP (1) | EP2721052A1 (es) |
| JP (1) | JP2014529330A (es) |
| KR (1) | KR20140033126A (es) |
| CN (1) | CN103619868A (es) |
| AR (1) | AR086938A1 (es) |
| AU (1) | AU2012269240B2 (es) |
| BR (1) | BR112013031906A2 (es) |
| CA (1) | CA2838476A1 (es) |
| CL (1) | CL2013003596A1 (es) |
| EA (1) | EA201400024A1 (es) |
| IL (1) | IL229763A0 (es) |
| MX (1) | MX2013014615A (es) |
| WO (1) | WO2012171853A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9994609B2 (en) | 2013-03-15 | 2018-06-12 | Biogen Ma Inc. | Hydrophobic interaction protein chromatography under no-salt conditions |
| KR102140531B1 (ko) * | 2018-08-07 | 2020-08-04 | (주)휴온스 | Gly-Tβ4의 제조방법 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2272870T3 (da) * | 1998-06-09 | 2013-08-05 | Csl Behring Ag | Fremgangsmåde til fremstilling af immunoglobuliner med henblik på intravenøs indgivelse og andre immunoglobulin-lignende produkter. |
| WO2005044856A2 (en) | 2003-10-27 | 2005-05-19 | Wyeth | Removal of high molecular weight aggregates using hydroxyapatite chromatography |
| DE10355251A1 (de) * | 2003-11-26 | 2005-06-23 | Merck Patent Gmbh | Pharmazeutische Zubereitung enthaltend einen Antikörper gegen den EGF-Rezeptor |
| NZ548126A (en) | 2004-02-27 | 2009-10-30 | Ge Healthcare Bio Sciences Ab | A process for the purification of antibodies involving addition of a second resin |
| US20110257378A1 (en) | 2008-11-25 | 2011-10-20 | Ge Healthcare Bio-Sciences Ab | Aqueous two phase extraction augmented precipitation process for purification of therapeutic proteins |
| JP5791602B2 (ja) * | 2009-08-07 | 2015-10-07 | イー・エム・デイー・ミリポア・コーポレイシヨン | 試料中の１つ以上の不純物から標的タンパク質を精製するための方法 |
| US9527010B2 (en) * | 2009-09-25 | 2016-12-27 | Ge Healthcare Bio-Sciences Corp. | Separation system and method |
-
2012
- 2012-06-08 EP EP12729090.6A patent/EP2721052A1/en not_active Withdrawn
- 2012-06-08 CN CN201280029638.7A patent/CN103619868A/zh active Pending
- 2012-06-08 WO PCT/EP2012/060885 patent/WO2012171853A1/en active Application Filing
- 2012-06-08 EA EA201400024A patent/EA201400024A1/ru unknown
- 2012-06-08 AU AU2012269240A patent/AU2012269240B2/en not_active Expired - Fee Related
- 2012-06-08 MX MX2013014615A patent/MX2013014615A/es unknown
- 2012-06-08 BR BR112013031906A patent/BR112013031906A2/pt not_active IP Right Cessation
- 2012-06-08 US US14/126,677 patent/US20140187749A1/en not_active Abandoned
- 2012-06-08 CA CA2838476A patent/CA2838476A1/en not_active Abandoned
- 2012-06-08 KR KR1020137033190A patent/KR20140033126A/ko not_active Application Discontinuation
- 2012-06-08 JP JP2014515142A patent/JP2014529330A/ja not_active Withdrawn
- 2012-06-14 AR ARP120102118A patent/AR086938A1/es unknown
-
2013
- 2013-12-02 IL IL229763A patent/IL229763A0/en unknown
- 2013-12-16 CL CL2013003596A patent/CL2013003596A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20140187749A1 (en) | 2014-07-03 |
| JP2014529330A (ja) | 2014-11-06 |
| IL229763A0 (en) | 2014-01-30 |
| CN103619868A (zh) | 2014-03-05 |
| EP2721052A1 (en) | 2014-04-23 |
| AU2012269240A1 (en) | 2013-05-02 |
| KR20140033126A (ko) | 2014-03-17 |
| EA201400024A1 (ru) | 2014-04-30 |
| AU2012269240B2 (en) | 2016-01-21 |
| CL2013003596A1 (es) | 2014-06-06 |
| BR112013031906A2 (pt) | 2016-12-13 |
| MX2013014615A (es) | 2014-02-17 |
| CA2838476A1 (en) | 2012-12-20 |
| WO2012171853A1 (en) | 2012-12-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |