AR086525A1 - Metodo de purificacion de anticuerpos - Google Patents
Metodo de purificacion de anticuerposInfo
- Publication number
- AR086525A1 AR086525A1 ARP120101815A ARP120101815A AR086525A1 AR 086525 A1 AR086525 A1 AR 086525A1 AR P120101815 A ARP120101815 A AR P120101815A AR P120101815 A ARP120101815 A AR P120101815A AR 086525 A1 AR086525 A1 AR 086525A1
- Authority
- AR
- Argentina
- Prior art keywords
- solution
- buffer
- obtaining
- acetic acid
- nacl
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
Reivindicación 1: Un método para purificar una proteína de una disolución que comprende: (a) una primera etapa de cromatografía que comprende: pasar dicha disolución sobre una primera columna de cromatografía; eluir un eluyente de proteína cruda de la primera columna de cromatografía usando un primer tampón de elución; y (b) una segunda etapa de cromatografía que comprende: pasar el eluyente de proteína cruda obtenido al final de la etapa (a) sobre una segunda columna de cromatografía; recuperar la proteína purificada de la segunda columna de cromatografía usando un segundo tampón de elusión, en el que cada uno de los tampones comprende Bis Tris.Reivindicación 38: Un método para preparar tampones adecuados para usarse en el método de una cualquiera de las reivindicaciones 1 a 37, que comprende las etapas de: i) crear una disolución con una concentración final de 15 a 25 mM Bis Tris y de 15 a 25 mM NaCl; ii) ajustar el pH de la disolución a un valor comprendido entre 7 y 8 con ácido acético; iii) recoger la mitad de la disolución, obteniendo de esta manera un tampón de equilibrado; iv) ajustar el pH de la mitad restante de la disolución de la etapa (iii) a un valor comprendido entre 4 y 5 con ácido acético; v) recoger la mitad de la disolución obtenida en la etapa (iv), obteniendo de esta manera un tampón de elusión; vi) ajustar el pH de la mitad restante de la disolución de la etapa (v) a un valor comprendido entre 3 y 4 con ácido acético, obteniendo de esta manera un tampón de elusión más; vii) recoger la mitad del tampón de equilibrado obtenido en la etapa (iii) y añadir NaCl para obtener una concentración final de NaCl comprendida entre 0,9 a 1,1 mM, obteniendo de esta manera un tampón de lavado y limpieza. Reivindicación 42: Uso de un tampón que comprende o consiste en Bis Tris, ácido acético, NaCl, y agua para purificar una proteína de una disolución por al menos una etapa de cromatografía.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/EP2011/070768 WO2013075740A1 (en) | 2011-11-23 | 2011-11-23 | Antibody purification method |
Publications (1)
Publication Number | Publication Date |
---|---|
AR086525A1 true AR086525A1 (es) | 2013-12-18 |
Family
ID=46125462
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP120101815A AR086525A1 (es) | 2011-11-23 | 2012-05-23 | Metodo de purificacion de anticuerpos |
Country Status (14)
Country | Link |
---|---|
US (2) | US10131714B2 (es) |
JP (2) | JP6437311B2 (es) |
KR (1) | KR102048598B1 (es) |
CN (1) | CN104039808B (es) |
AR (1) | AR086525A1 (es) |
AU (2) | AU2012342865A1 (es) |
BR (1) | BR112014012275A2 (es) |
CA (1) | CA2855491A1 (es) |
IL (1) | IL232558B (es) |
MX (1) | MX364908B (es) |
RU (1) | RU2603347C2 (es) |
SG (1) | SG11201402410RA (es) |
TW (1) | TWI643865B (es) |
WO (2) | WO2013075740A1 (es) |
Families Citing this family (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013075740A1 (en) | 2011-11-23 | 2013-05-30 | Sanofi | Antibody purification method |
US10704021B2 (en) | 2012-03-15 | 2020-07-07 | Flodesign Sonics, Inc. | Acoustic perfusion devices |
DK3301115T3 (da) * | 2013-05-06 | 2022-01-31 | Sanofi Sa | Kontinuerlig flertrinsfremgangsmåde til oprensning af antistoffer |
AR096713A1 (es) | 2013-06-25 | 2016-01-27 | Cadila Healthcare Ltd | Proceso de purificación para anticuerpos monoclonales |
CN103480346B (zh) * | 2013-10-08 | 2016-04-06 | 天津工业大学 | 一种含大环化合物的新型亲和分离材质制备方法及应用 |
CN104628846B (zh) * | 2013-11-06 | 2019-12-06 | 三生国健药业(上海)股份有限公司 | 重组蛋白质的纯化方法 |
WO2015105955A1 (en) | 2014-01-08 | 2015-07-16 | Flodesign Sonics, Inc. | Acoustophoresis device with dual acoustophoretic chamber |
US10329323B2 (en) * | 2014-07-25 | 2019-06-25 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Method for purifying antibodies using PBS |
US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
WO2016106291A1 (en) * | 2014-12-22 | 2016-06-30 | Alexion Pharmaceuticals, Inc. | Methods of purifying recombinant proteins |
US11708572B2 (en) | 2015-04-29 | 2023-07-25 | Flodesign Sonics, Inc. | Acoustic cell separation techniques and processes |
US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
US11214789B2 (en) | 2016-05-03 | 2022-01-04 | Flodesign Sonics, Inc. | Concentration and washing of particles with acoustics |
US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
WO2017194597A1 (en) | 2016-05-11 | 2017-11-16 | Ge Healthcare Bioprocess R&D Ab | Separation matrix |
US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
CN109311949B (zh) | 2016-05-11 | 2022-09-16 | 思拓凡生物工艺研发有限公司 | 储存分离基质的方法 |
EP3455241B1 (en) | 2016-05-11 | 2022-02-23 | Cytiva BioProcess R&D AB | Method of cleaning and/or sanitizing a separation matrix |
US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
US11760777B2 (en) | 2017-04-26 | 2023-09-19 | Bristol-Myers Squibb Company | Methods of antibody production that minimize disulfide bond reduction |
CA3085784A1 (en) | 2017-12-14 | 2019-06-20 | Flodesign Sonics, Inc. | Acoustic transducer driver and controller |
US11603527B2 (en) * | 2017-12-27 | 2023-03-14 | Global Life Sciences Solutions Usa Llc | Method and kit for viral vector isolation |
WO2019183334A1 (en) | 2018-03-21 | 2019-09-26 | Waters Technologies Corporation | Non-antibody high-affinity-based sample preparation, sorbents, devices and methods |
EP3546475A1 (en) * | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
CN112105927A (zh) * | 2018-05-08 | 2020-12-18 | 沃特世科技公司 | 可用于pH梯度阳离子交换色谱法的方法、组合物和试剂盒 |
EP3636657A1 (en) | 2018-10-08 | 2020-04-15 | Ablynx N.V. | Chromatography-free antibody purification method |
CN113845562A (zh) * | 2020-06-28 | 2021-12-28 | 佛山汉腾生物科技有限公司 | 目的蛋白纯化方法 |
WO2022234412A1 (en) | 2021-05-03 | 2022-11-10 | Lupin Limited | A process for purification of fc-fusion proteins |
US20230240961A1 (en) * | 2022-01-28 | 2023-08-03 | Praj Industries Limited | Process for the preparation of antimalassezia powder |
Family Cites Families (28)
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KR930008447B1 (ko) * | 1987-10-23 | 1993-09-04 | 쉐링 코포레이션 | 단백질 정제방법 |
CA2106612C (en) | 1993-09-21 | 2001-02-06 | Diana Pliura | Displacement chromatography process |
US5891741A (en) * | 1997-05-16 | 1999-04-06 | Coulter International Corp. | Antibody-aminodextran-phycobiliprotein conjugates |
CN1136317C (zh) * | 1999-03-12 | 2004-01-28 | 中国人民解放军第四军医大学 | 单克隆抗体双段和单段的制备方法 |
US20020009445A1 (en) * | 2000-07-12 | 2002-01-24 | Yansheng Du | Human beta-amyloid antibody and use thereof for treatment of alzheimer's disease |
US6602855B2 (en) * | 2001-04-30 | 2003-08-05 | Syn X Pharma, Inc. | Biopolymer marker indicative of disease state having a molecular weight of 1449 daltons |
SI1745075T1 (sl) | 2004-04-21 | 2013-07-31 | The Brigham And Women's Hospital, Inc. | Poli-N-acetilglukozamin (PNAG/dPNAG) vezavni peptidi in postopki za njihovo uporabo |
CA2583578C (en) | 2004-10-21 | 2014-04-01 | Ge Healthcare Bio-Sciences Ab | Chromatography ligand |
EP3312196B1 (en) | 2005-03-23 | 2019-07-17 | Genmab A/S | Antibodies against cd38 for treatment of multiple myeloma |
CN1814775A (zh) * | 2005-12-06 | 2006-08-09 | 中国海洋大学 | 一种抗病毒活性寡聚肽的制备方法 |
EP1914242A1 (en) * | 2006-10-19 | 2008-04-23 | Sanofi-Aventis | Novel anti-CD38 antibodies for the treatment of cancer |
ES2364118T3 (es) | 2007-07-11 | 2011-08-25 | Novo Nordisk A/S | Purificación de factor viii usando una resina de modo mixto o multimodal. |
MY158903A (en) | 2007-11-16 | 2016-11-30 | Univ Rockefeller | Antibodies specific for the protofibril form of beta-amyloid protein |
KR20100128315A (ko) * | 2008-02-29 | 2010-12-07 | 바이오겐 아이덱 엠에이 인코포레이티드 | 정제된 면역글로불린 융합 단백질 및 그의 정제 방법 |
WO2010009388A1 (en) | 2008-07-18 | 2010-01-21 | Talecris Biotherapeutics, Inc. | Method of preparing alpha-1 proteinase inhibitor |
JPWO2010071208A1 (ja) * | 2008-12-19 | 2012-05-31 | 武田薬品工業株式会社 | 抗体の精製方法 |
AU2010205007A1 (en) | 2009-01-13 | 2011-07-07 | Ge Healthcare Bio-Sciences Ab | Precipitation of biomolecules with negatively charged polymers |
KR101224468B1 (ko) | 2009-05-20 | 2013-01-23 | 주식회사 파멥신 | 신규한 형태의 이중표적항체 및 그 용도 |
ES2817802T3 (es) | 2009-08-07 | 2021-04-08 | Emd Millipore Corp | Métodos para purificar una proteína objetivo de una o más impurezas en una muestra |
US9527010B2 (en) | 2009-09-25 | 2016-12-27 | Ge Healthcare Bio-Sciences Corp. | Separation system and method |
WO2011049798A1 (en) | 2009-10-20 | 2011-04-28 | Merck Sharp & Dohme Corp. | Use of mixed mode chromatography for the capture and purification of basic antibody products |
ES2534936T3 (es) * | 2010-08-02 | 2015-04-30 | Ratiopharm Gmbh | Método para producir y purificar una sialiltransferasa soluble activa |
US20120238730A1 (en) | 2011-03-15 | 2012-09-20 | Abbott Laboratories | Integrated approach to the isolation and purification of antibodies |
WO2012135415A1 (en) * | 2011-03-29 | 2012-10-04 | Glaxosmithkline Llc | Buffer system for protein purification |
CN107421796B (zh) * | 2011-06-17 | 2021-05-04 | 罗氏血液诊断股份有限公司 | 用于生物样品的组织加工的溶液 |
US9096648B2 (en) | 2011-08-19 | 2015-08-04 | Emd Millipore Corporation | Methods of reducing level of one or more impurities in a sample during protein purification |
CN104023743B (zh) | 2011-10-25 | 2017-05-03 | 普罗西纳治疗有限公司 | 抗体制剂和方法 |
WO2013075740A1 (en) | 2011-11-23 | 2013-05-30 | Sanofi | Antibody purification method |
-
2011
- 2011-11-23 WO PCT/EP2011/070768 patent/WO2013075740A1/en active Application Filing
-
2012
- 2012-05-23 JP JP2014542734A patent/JP6437311B2/ja active Active
- 2012-05-23 SG SG11201402410RA patent/SG11201402410RA/en unknown
- 2012-05-23 AR ARP120101815A patent/AR086525A1/es unknown
- 2012-05-23 MX MX2014006284A patent/MX364908B/es active IP Right Grant
- 2012-05-23 US US14/360,103 patent/US10131714B2/en active Active
- 2012-05-23 WO PCT/EP2012/059528 patent/WO2013075849A1/en active Application Filing
- 2012-05-23 CN CN201280067326.5A patent/CN104039808B/zh active Active
- 2012-05-23 TW TW101118395A patent/TWI643865B/zh not_active IP Right Cessation
- 2012-05-23 CA CA2855491A patent/CA2855491A1/en active Pending
- 2012-05-23 KR KR1020147016670A patent/KR102048598B1/ko active IP Right Grant
- 2012-05-23 BR BR112014012275A patent/BR112014012275A2/pt not_active Application Discontinuation
- 2012-05-23 RU RU2014125063/04A patent/RU2603347C2/ru active
- 2012-05-23 AU AU2012342865A patent/AU2012342865A1/en not_active Abandoned
-
2014
- 2014-05-12 IL IL232558A patent/IL232558B/en active IP Right Grant
-
2017
- 2017-01-20 JP JP2017008139A patent/JP2017095505A/ja active Pending
- 2017-10-16 AU AU2017245478A patent/AU2017245478B2/en active Active
-
2018
- 2018-10-04 US US16/151,963 patent/US20190135942A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
CN104039808A (zh) | 2014-09-10 |
US20140323698A1 (en) | 2014-10-30 |
SG11201402410RA (en) | 2014-06-27 |
MX364908B (es) | 2019-05-13 |
TWI643865B (zh) | 2018-12-11 |
RU2603347C2 (ru) | 2016-11-27 |
AU2017245478B2 (en) | 2019-05-16 |
AU2012342865A1 (en) | 2014-06-19 |
US20190135942A1 (en) | 2019-05-09 |
KR102048598B1 (ko) | 2019-11-25 |
RU2014125063A (ru) | 2015-12-27 |
MX2014006284A (es) | 2014-10-24 |
KR20140094013A (ko) | 2014-07-29 |
WO2013075849A1 (en) | 2013-05-30 |
JP2017095505A (ja) | 2017-06-01 |
US10131714B2 (en) | 2018-11-20 |
JP6437311B2 (ja) | 2018-12-12 |
TW201321402A (zh) | 2013-06-01 |
BR112014012275A2 (pt) | 2017-05-30 |
CN104039808B (zh) | 2019-04-19 |
IL232558A0 (en) | 2014-06-30 |
JP2015500798A (ja) | 2015-01-08 |
AU2017245478A1 (en) | 2017-11-02 |
IL232558B (en) | 2018-08-30 |
CA2855491A1 (en) | 2013-05-30 |
WO2013075740A1 (en) | 2013-05-30 |
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