BR112014027994A2 - métodos de cristalização para purificação de anticorpos monoclonais - Google Patents

métodos de cristalização para purificação de anticorpos monoclonais

Info

Publication number
BR112014027994A2
BR112014027994A2 BR112014027994A BR112014027994A BR112014027994A2 BR 112014027994 A2 BR112014027994 A2 BR 112014027994A2 BR 112014027994 A BR112014027994 A BR 112014027994A BR 112014027994 A BR112014027994 A BR 112014027994A BR 112014027994 A2 BR112014027994 A2 BR 112014027994A2
Authority
BR
Brazil
Prior art keywords
monoclonal antibodies
methods
monoclonal antibody
crystallization methods
antibody purification
Prior art date
Application number
BR112014027994A
Other languages
English (en)
Inventor
Smejkal Benjamin
Helk Bernhard
Hekmat Dariusch
Konrad Schulz Henk
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of BR112014027994A2 publication Critical patent/BR112014027994A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D9/00—Crystallisation
    • B01D9/005—Selection of auxiliary, e.g. for control of crystallisation nuclei, of crystal growth, of adherence to walls; Arrangements for introduction thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/16—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Botany (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

resumo patente de invenção: "métodos de cristalização para purificação de anticorpos monoclonais". a presente invenção refere-se a métodos inventivos que resolvem problemas tipicamente encontrados durante a purificação de anticorpos monoclonais. os métodos descritos neste relatório são, surpreendentemente, úteis para proporcionar preparações de anticorpos monoclonais purificados a partir de misturas que compreendem anticorpos monoclonais. em algumas modalidades, os métodos da descrição descritos neste relatório proporcionam para a produção de alta pureza, anticorpos monoclonais cristalizados em alto rendimento diretamente, a partir de sobrenadante de cultura livre de células.
BR112014027994A 2012-05-11 2013-05-10 métodos de cristalização para purificação de anticorpos monoclonais BR112014027994A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261645855P 2012-05-11 2012-05-11
PCT/EP2013/059696 WO2013167720A1 (en) 2012-05-11 2013-05-10 Crystallization methods for purification of monoclonal antibodies

Publications (1)

Publication Number Publication Date
BR112014027994A2 true BR112014027994A2 (pt) 2017-06-27

Family

ID=48326319

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014027994A BR112014027994A2 (pt) 2012-05-11 2013-05-10 métodos de cristalização para purificação de anticorpos monoclonais

Country Status (14)

Country Link
US (2) US20150133642A1 (pt)
EP (1) EP2846830A1 (pt)
JP (1) JP2015517306A (pt)
KR (1) KR20150016503A (pt)
CN (1) CN104284676B (pt)
AU (1) AU2013258006B2 (pt)
BR (1) BR112014027994A2 (pt)
CA (1) CA2872145A1 (pt)
IL (1) IL235483A0 (pt)
IN (1) IN2014DN09097A (pt)
MX (1) MX2014013754A (pt)
RU (1) RU2014150071A (pt)
SG (1) SG11201406664SA (pt)
WO (1) WO2013167720A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3015542A1 (de) * 2015-05-07 2016-05-04 Bayer Technology Services GmbH Modulare anlage und verfahren zur kontinuierlichen, keimreduzierten produktion und/oder aufbereitung eines produktes
MY191508A (en) * 2016-12-23 2022-06-28 Arla Foods Amba Production of beta-lactoglobulin preparations
EP3964519A1 (en) * 2017-03-14 2022-03-09 Amgen Inc. Methods directed to crystalline biomolecules
IT202100004496A1 (it) 2021-02-25 2022-08-25 Univ Della Calabria Recupero di farmaci biologici o loro frammenti da soluzioni impure mediante cristallizzazione o precipitazione con membrane
CN116949036B (zh) * 2023-09-11 2023-12-15 成都斯马特科技有限公司 一种快速从腹水中提取核酸的试剂盒及提取方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7833525B2 (en) * 2000-12-28 2010-11-16 Bhami Shenoy Crystals of whole antibodies and fragments thereof and methods for making and using them
EP1450847B1 (en) * 2001-11-13 2010-09-29 Genentech, Inc. APO2 ligand/ TRAIL formulations and uses thereof
US7087719B2 (en) * 2002-11-19 2006-08-08 Gtc Biotherapeutics, Inc. Method for the crystallization of human serum albumin
AU2008343347A1 (en) * 2007-12-21 2009-07-09 Genentech, Inc. Crystallization of anti-CD20 antibodies
CN101320006B (zh) * 2008-07-17 2010-12-01 西北工业大学 蛋白质结晶条件的筛选方法

Also Published As

Publication number Publication date
KR20150016503A (ko) 2015-02-12
IN2014DN09097A (pt) 2015-05-22
US20170198028A1 (en) 2017-07-13
IL235483A0 (en) 2014-12-31
CN104284676A (zh) 2015-01-14
SG11201406664SA (en) 2014-11-27
EP2846830A1 (en) 2015-03-18
RU2014150071A (ru) 2016-07-10
CN104284676B (zh) 2018-11-27
AU2013258006A1 (en) 2014-11-06
AU2013258006B2 (en) 2016-04-28
US20150133642A1 (en) 2015-05-14
MX2014013754A (es) 2015-02-12
CA2872145A1 (en) 2013-11-14
JP2015517306A (ja) 2015-06-22
WO2013167720A1 (en) 2013-11-14

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Legal Events

Date Code Title Description
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements