ES2492681T3 - Método cromatográfico para purificar proteínas que contienen FC - Google Patents
Método cromatográfico para purificar proteínas que contienen FC Download PDFInfo
- Publication number
- ES2492681T3 ES2492681T3 ES11700457.2T ES11700457T ES2492681T3 ES 2492681 T3 ES2492681 T3 ES 2492681T3 ES 11700457 T ES11700457 T ES 11700457T ES 2492681 T3 ES2492681 T3 ES 2492681T3
- Authority
- ES
- Spain
- Prior art keywords
- protein
- concentration
- sought
- mobile phase
- stationary phase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 8
- 108090000623 proteins and genes Proteins 0.000 title abstract 8
- 238000004587 chromatography analysis Methods 0.000 title abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 4
- 230000005526 G1 to G0 transition Effects 0.000 abstract 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 abstract 2
- 239000004475 Arginine Substances 0.000 abstract 1
- 108060003951 Immunoglobulin Proteins 0.000 abstract 1
- 239000000654 additive Substances 0.000 abstract 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 239000012149 elution buffer Substances 0.000 abstract 1
- 102000018358 immunoglobulin Human genes 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 abstract 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 abstract 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 239000011534 wash buffer Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
- A61K39/39591—Stabilisation, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
Abstract
Método para disminuir las impurezas de una composición que contiene una proteína con el dominio Fc de una inmunoglobulina (proteína buscada) mediante una cromatografía de proteína A que consta de las siguientes etapas: a) uso de una fase móvil que contiene la proteína buscada sobre una fase estacionaria que lleva la proteína A, en unas condiciones en que la proteína buscada se fija a la fase estacionaria; b) uso de un tampón de lavado con un pH comprendido entre 5 y 8 como fase móvil, que lleva como aditivos i) arginina a una concentración de 0,1 - 1 mol/l, ii) cloruro sódico a una concentración de 0,2 hasta 2 mol/l, iii) un alcohol elegido del grupo formado por isopropanol, n-propanol y etanol a una concentración del 5 - 30% (p/v) y iv) polivinilpirrolidona o un detergente a una concentración del 0,05 - 2% (p/v); c) uso de un tampón de elución como fase móvil, en unas condiciones en que la proteína buscada es eluida de la fase estacionaria.
Description
Claims (1)
-
imagen1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10151416 | 2010-01-22 | ||
| EP10151416 | 2010-01-22 | ||
| EP10171975 | 2010-08-05 | ||
| EP10171975 | 2010-08-05 | ||
| PCT/EP2011/050817 WO2011089212A1 (de) | 2010-01-22 | 2011-01-21 | Chromatographisches verfahren zur aufreinigung von fc enthaltenden proteinen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2492681T3 true ES2492681T3 (es) | 2014-09-10 |
Family
ID=43598239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES11700457.2T Active ES2492681T3 (es) | 2010-01-22 | 2011-01-21 | Método cromatográfico para purificar proteínas que contienen FC |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US9284347B2 (es) |
| EP (1) | EP2526114B1 (es) |
| JP (1) | JP5567691B2 (es) |
| KR (1) | KR101753569B1 (es) |
| CN (1) | CN102712673B (es) |
| AU (1) | AU2011208659B2 (es) |
| CA (1) | CA2784959C (es) |
| DK (1) | DK2526114T3 (es) |
| ES (1) | ES2492681T3 (es) |
| SG (1) | SG182553A1 (es) |
| WO (1) | WO2011089212A1 (es) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ602971A (en) * | 2010-04-23 | 2014-11-28 | Serum Inst India Ltd | Simple method for simultaneous removal of multiple impurities from culture supernatants to ultralow levels |
| EP2714714B1 (en) * | 2011-06-01 | 2018-06-20 | Novartis AG | Wash solution and method for affinity chromatography |
| RU2646098C2 (ru) | 2013-04-30 | 2018-03-01 | Интас Фармасьютикалс Лтд | Новый способ клонирования, экспрессии и очистки для получения ранибизумаба |
| AR096713A1 (es) | 2013-06-25 | 2016-01-27 | Cadila Healthcare Ltd | Proceso de purificación para anticuerpos monoclonales |
| AU2015273049B2 (en) * | 2014-06-13 | 2019-11-14 | Lupin Limited | Process for the purification of TNFR:Fc fusion protein |
| US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| US10988527B2 (en) | 2014-12-31 | 2021-04-27 | Lg Chem, Ltd. | Method for preparing TNFR-Fc fusion protein containing target content of impurities |
| US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| CN109311949B (zh) | 2016-05-11 | 2022-09-16 | 思拓凡生物工艺研发有限公司 | 储存分离基质的方法 |
| US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
| US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| JP7031934B2 (ja) | 2016-05-11 | 2022-03-08 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックス |
| EP3455243B1 (en) | 2016-05-11 | 2021-03-24 | Cytiva BioProcess R&D AB | Separation matrix |
| ES2909833T3 (es) | 2016-05-11 | 2022-05-10 | Cytiva Bioprocess R & D Ab | Método de limpieza y/o desinfección de una matriz de separación |
| US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| US12448411B2 (en) | 2016-09-30 | 2025-10-21 | Cytiva Bioprocess R&D Ab | Separation method |
| WO2019096777A1 (en) * | 2017-11-14 | 2019-05-23 | Bio-Sourcing S.A. | Antibody purification |
| EP3546475A1 (en) * | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| WO2020125757A1 (en) * | 2018-12-21 | 2020-06-25 | Wuxi Biologics (Shanghai) Co., Ltd. | A method for improving aggregate removal by protein a chromatography |
| CN111333776B (zh) * | 2020-03-20 | 2021-11-09 | 暨南大学 | 一种氮杂环类有机聚合物整体材料及制备与应用 |
| WO2024017826A2 (en) * | 2022-07-19 | 2024-01-25 | Glaxosmithkline Intellectual Property Development Limited | Methods for purifying recombinant polypeptides |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5874218A (en) * | 1990-06-11 | 1999-02-23 | Nexstar Pharmaceuticals, Inc. | Method for detecting a target compound in a substance using a nucleic acid ligand |
| US5585246A (en) * | 1993-02-17 | 1996-12-17 | Biometric Imaging, Inc. | Method for preparing a sample in a scan capillary for immunofluorescent interrogation |
| CA2473144C (en) | 2002-02-05 | 2013-05-28 | Genentech, Inc. | Protein purification |
| US8084032B2 (en) * | 2004-01-21 | 2011-12-27 | Ajinomoto Co., Inc. | Purification method which prevents denaturation of an antibody |
| US20070172846A1 (en) * | 2005-11-12 | 2007-07-26 | Introgen Therapeutics, Inc. | Methods for the Production and Purification of Adenoviral Vectors |
| AU2007204987B2 (en) | 2006-01-06 | 2011-11-24 | Amgen Inc. | Methods and systems for isolating target molecules from complex solutions by column-chromatography using eluants containing organic solvents |
| WO2007109163A2 (en) | 2006-03-16 | 2007-09-27 | Amgen Inc | Wash buffer and method of using |
| WO2008031020A2 (en) * | 2006-09-08 | 2008-03-13 | Wyeth | Arginine wash in protein purification using affinity chromatography |
| AU2010260476A1 (en) * | 2009-03-11 | 2011-10-13 | Wyeth Llc | Methods of purifying small modular immunopharmaceutical proteins |
| CA2784278C (en) * | 2009-12-18 | 2019-02-26 | Novartis Ag | Wash solution and method for affinity chromatography |
-
2011
- 2011-01-21 US US13/522,030 patent/US9284347B2/en active Active
- 2011-01-21 EP EP11700457.2A patent/EP2526114B1/de active Active
- 2011-01-21 AU AU2011208659A patent/AU2011208659B2/en active Active
- 2011-01-21 SG SG2012052452A patent/SG182553A1/en unknown
- 2011-01-21 CN CN201180006652.0A patent/CN102712673B/zh active Active
- 2011-01-21 KR KR1020127019071A patent/KR101753569B1/ko active Active
- 2011-01-21 CA CA2784959A patent/CA2784959C/en active Active
- 2011-01-21 JP JP2012549368A patent/JP5567691B2/ja active Active
- 2011-01-21 ES ES11700457.2T patent/ES2492681T3/es active Active
- 2011-01-21 WO PCT/EP2011/050817 patent/WO2011089212A1/de not_active Ceased
- 2011-01-21 DK DK11700457.2T patent/DK2526114T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| JP5567691B2 (ja) | 2014-08-06 |
| CA2784959A1 (en) | 2011-07-28 |
| WO2011089212A1 (de) | 2011-07-28 |
| CN102712673B (zh) | 2014-04-30 |
| KR101753569B1 (ko) | 2017-07-04 |
| JP2013517318A (ja) | 2013-05-16 |
| EP2526114A1 (de) | 2012-11-28 |
| KR20120118013A (ko) | 2012-10-25 |
| CA2784959C (en) | 2018-06-26 |
| US9284347B2 (en) | 2016-03-15 |
| US20130197197A1 (en) | 2013-08-01 |
| HK1174044A1 (en) | 2013-05-31 |
| SG182553A1 (en) | 2012-08-30 |
| EP2526114B1 (de) | 2014-06-18 |
| AU2011208659B2 (en) | 2015-05-28 |
| CN102712673A (zh) | 2012-10-03 |
| DK2526114T3 (da) | 2014-09-29 |
| AU2011208659A1 (en) | 2012-07-19 |
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