AR064683A1 - Procedimiento para la extraccion de una proteina presente en la leche - Google Patents

Procedimiento para la extraccion de una proteina presente en la leche

Info

Publication number
AR064683A1
AR064683A1 ARP070105952A ARP070105952A AR064683A1 AR 064683 A1 AR064683 A1 AR 064683A1 AR P070105952 A ARP070105952 A AR P070105952A AR P070105952 A ARP070105952 A AR P070105952A AR 064683 A1 AR064683 A1 AR 064683A1
Authority
AR
Argentina
Prior art keywords
protein
milk
extraction
procedure
protein present
Prior art date
Application number
ARP070105952A
Other languages
English (en)
Inventor
Monique Ollivier
Michel Nogre
Alain Lejars
Original Assignee
Lfb Biotechnologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lfb Biotechnologies filed Critical Lfb Biotechnologies
Publication of AR064683A1 publication Critical patent/AR064683A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Transplantation (AREA)
  • Reproductive Health (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Dairy Products (AREA)

Abstract

La presente solicitud se refiere a un procedimiento para la extraccion de una proteína presente en la leche, que tiene por lo menos, incluyendo los siguientes pasos de a) descremar y desgrasar dicha leche, b) transferir la fraccion desgrasada y descremada que contiene dicha proteína a un sopote cromatográfico sobre el cual está injertado un ligando que es simultáneamente hidrofobo y ionico bajo condiciones de pH que permiten que dicha proteína sea capturada sobre dicho soporte, siendo dicho pH mayor que 4,6, c) eluir la proteína, d) purificar la fraccion eluida retirando las proteínas de la leche de dicha fraccion eluida, y e) recuperar dicha proteína.
ARP070105952A 2006-12-29 2007-12-28 Procedimiento para la extraccion de una proteina presente en la leche AR064683A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0611536A FR2910786B1 (fr) 2006-12-29 2006-12-29 "procede d'extraction d'une proteine presente dans du lait"

Publications (1)

Publication Number Publication Date
AR064683A1 true AR064683A1 (es) 2009-04-22

Family

ID=38182268

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP070105952A AR064683A1 (es) 2006-12-29 2007-12-28 Procedimiento para la extraccion de una proteina presente en la leche

Country Status (13)

Country Link
US (2) US8492524B2 (es)
EP (1) EP2134191A2 (es)
JP (1) JP5196590B2 (es)
KR (1) KR101294751B1 (es)
CN (2) CN101600358B (es)
AR (1) AR064683A1 (es)
AU (1) AU2008214544B2 (es)
BR (1) BRPI0805838A2 (es)
CA (1) CA2673583C (es)
FR (1) FR2910786B1 (es)
IL (1) IL199360A (es)
TW (2) TWI496542B (es)
WO (1) WO2008099077A2 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0416950B8 (pt) 2003-12-01 2021-05-25 Novo Nordisk Healthcare Ag métodos para remover vírus de uma composição do fator vii líquida, para inativar vírus em uma composição do fator vii líquida, e para eliminar em alto nível a presença de vírus ativos em uma composição do fator vii líquida.
FR2901707B1 (fr) 2006-05-31 2017-09-29 Lab Francais Du Fractionnement Composition de facteur vii recombinant ou transgenique, chaque molecule de facteur vii possedant deux sites de n-glycosylation a motifs glycanniques definis
FR2910786B1 (fr) * 2006-12-29 2017-08-11 Laboratoire Francais Du Fractionnement Et Des Biotechnologies (Lfb) "procede d'extraction d'une proteine presente dans du lait"
FR2942232B1 (fr) 2009-02-19 2015-03-13 Lfb Biotechnologies Moyens pour la purification d'une proteine de la coagulation et procedes pour sa mise en oeuvre
FR2942231B1 (fr) 2009-02-19 2015-03-20 Lfb Biotechnologies Acides nucleiques se liant specifiquement au facteur vii/viia humain, et utilisations
FR2942233B1 (fr) * 2009-02-19 2015-03-13 Lfb Biotechnologies Moyens pour la purification d'une proteine du plasma sanguin, et procedes pour sa mise en oeuvre
FR2947181B1 (fr) 2009-06-26 2012-05-04 Lfb Biotechnologies Composition de facteur vii
WO2011112075A1 (en) * 2010-03-11 2011-09-15 N.V. Nutricia Controlling the texture of high-protein nutritional compositions comprising micellar casein
US9364021B2 (en) * 2011-09-09 2016-06-14 Tairob Ltd. Method for preparing micro-foam whipped milk for cappuccino or a method for whipping other liquids containing proteins, using an apparatus
EP2687595B1 (en) * 2012-07-19 2018-05-30 Laboratoire Français du Fractionnement et des Biotechnologies Method for purifying transgenic factor VII
FR3006591B1 (fr) 2013-06-11 2016-05-06 Lab Francais Du Fractionnement Composition de facteur vii presentant un point isoelectrique substantiellement homogene
CN106793793A (zh) * 2014-10-06 2017-05-31 预层析股份有限公司 来自含有聚集的酪蛋白的混合物的可溶性蛋白的分离
FR3034419B1 (fr) * 2015-04-02 2017-12-15 Lab Francais Du Fractionnement Procede de purification d'une proteine recombinante therapeutique a partir d'un lait transgenique
CN110177878B (zh) * 2016-12-29 2023-11-03 Asc治疗公司 转基因动物和生物生产方法
FR3076294B1 (fr) 2017-12-29 2022-01-28 Lab Francais Du Fractionnement Procede de purification d'anticorps a partir de lait brut
GB201815053D0 (en) * 2018-09-14 2018-10-31 Dublin Institute Of Tech Method
US11326176B2 (en) * 2019-11-22 2022-05-10 Mozza Foods, Inc. Recombinant micelle and method of in vivo assembly
CN112159458A (zh) * 2020-09-28 2021-01-01 靡特洛 一种从过期变质牛奶中提取牛奶蛋白的方法

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US4229342A (en) * 1977-05-18 1980-10-21 Rhone-Poulenc Industries Process for extracting proteins from milk using silica and anion exchange resins
NL8204923A (nl) 1982-12-21 1984-07-16 Stichting Nl I Zuivelonderzoek Werkwijze voor het bereiden van een precipitaat van caseine en wei-eiwit alsmede aldus bereid precipitaat.
GR860984B (en) 1985-04-17 1986-08-18 Zymogenetics Inc Expression of factor vii and ix activities in mammalian cells
DE122007000007I2 (de) 1986-04-09 2010-12-30 Genzyme Corp Genetisch transformierte Tiere, die ein gewünschtes Protein in Milch absondern
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FR2677652B1 (fr) 1991-06-12 2005-05-27 Agronomique Inst Nat Rech Procede de preparation d'une proteine d'interet dans le lait d'un animal transgenique, produit obtenu et cellule eucaryote utilisee.
US5827690A (en) 1993-12-20 1998-10-27 Genzyme Transgenics Corporatiion Transgenic production of antibodies in milk
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US6713662B1 (en) 1994-07-27 2004-03-30 Pharming Intellectual Property B.V. Production of collagen in the milk of transgenic mammals
US5880327A (en) 1994-09-21 1999-03-09 American National Red Cross Transgenic mammals expressing human coagulation factor VIII
US6268487B1 (en) * 1996-05-13 2001-07-31 Genzyme Transgenics Corporation Purification of biologically active peptides from milk
US6183803B1 (en) * 1999-06-11 2001-02-06 Biosante Pharmaceuticals, Inc. Method for processing milk
ATE420108T1 (de) * 2001-07-20 2009-01-15 Campina Bv Verfahren zur herstellung von preparationen aus milchprodukten, welche wachstumsfaktoren (tgf- beta und igf-1), lactoperoxidase und immunglobuline mit niedriger gegenseitiger verunreinigung enthalten
US20040117862A1 (en) * 2002-03-11 2004-06-17 Cooper Julian D. Production of high levels of transgenic factor VII with engineered stability and its therapeutic uses
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US6911323B2 (en) * 2002-09-25 2005-06-28 Novo Nordisk Healthcare A/G Human coagulation factor VII polypeptides
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JP2008500972A (ja) * 2004-05-14 2008-01-17 キリンファーマ株式会社 免疫グロブリンの精製方法
KR20080006601A (ko) * 2005-04-11 2008-01-16 메다렉스, 인코포레이티드 단백질 정제 방법
FR2910786B1 (fr) * 2006-12-29 2017-08-11 Laboratoire Francais Du Fractionnement Et Des Biotechnologies (Lfb) "procede d'extraction d'une proteine presente dans du lait"

Also Published As

Publication number Publication date
CN101600358A (zh) 2009-12-09
EP2134191A2 (fr) 2009-12-23
IL199360A (en) 2013-05-30
CA2673583A1 (en) 2008-08-21
JP2010514746A (ja) 2010-05-06
TWI519239B (zh) 2016-02-01
FR2910786A1 (fr) 2008-07-04
AU2008214544A1 (en) 2008-08-21
BRPI0805838A2 (pt) 2011-08-30
JP5196590B2 (ja) 2013-05-15
CA2673583C (en) 2013-06-11
CN101600358B (zh) 2014-05-07
TW200838437A (en) 2008-10-01
US8492524B2 (en) 2013-07-23
TWI496542B (zh) 2015-08-21
WO2008099077A2 (fr) 2008-08-21
WO2008099077A3 (fr) 2009-05-07
US20090281283A1 (en) 2009-11-12
KR101294751B1 (ko) 2013-08-12
TW201350024A (zh) 2013-12-16
FR2910786B1 (fr) 2017-08-11
CN103910778A (zh) 2014-07-09
KR20090113825A (ko) 2009-11-02
IL199360A0 (en) 2010-03-28
AU2008214544B2 (en) 2013-06-20
US20130295646A1 (en) 2013-11-07

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Legal Events

Date Code Title Description
FG Grant, registration
FD Application declared void or lapsed, e.g., due to non-payment of fee