BRPI0805838A2 - procedimento para a extração de uma proteìna presente no leite - Google Patents

procedimento para a extração de uma proteìna presente no leite Download PDF

Info

Publication number
BRPI0805838A2
BRPI0805838A2 BRPI0805838-5A BRPI0805838A BRPI0805838A2 BR PI0805838 A2 BRPI0805838 A2 BR PI0805838A2 BR PI0805838 A BRPI0805838 A BR PI0805838A BR PI0805838 A2 BRPI0805838 A2 BR PI0805838A2
Authority
BR
Brazil
Prior art keywords
protein
milk
procedure
extracting
extraction
Prior art date
Application number
BRPI0805838-5A
Other languages
English (en)
Inventor
Michel Nogre
Alain Lejars
Monique Ollivier
Original Assignee
Lfb Biotechnologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lfb Biotechnologies filed Critical Lfb Biotechnologies
Publication of BRPI0805838A2 publication Critical patent/BRPI0805838A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Transplantation (AREA)
  • Public Health (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Dairy Products (AREA)

Abstract

PROCEDIMENTO PARA A EXTRAçãO DE UMA PROTEìNA PRESENTE NO LEITE. A invenção refere-se a um procedimento de extração de uma proteína presente no leite e que apresenta pelo menos uma bolsa hidrofóbica e uma carga negativa de com ph natural do leite, compreendendo as seguintes etapas: a) Desnatação e delipidação do referido leite, b) Passagem da fração delipidada e desnatada contendo a referida proteína em um suporte cromatográfico no qual esta enxertado um ligante que apresenta ao mesmo tempo um caráter hidrofóbico e um caráter iónico em condições de pH que permitem à referida proteína ser retida no referido suporte, o pH estando superior a 4,6, c) Eluição da proteína, d) Purificação da fração eluída por eliminação das proteínas do leite da referida fração eluída, e e) Recuperação da referida proteína.
BRPI0805838-5A 2006-12-29 2008-01-02 procedimento para a extração de uma proteìna presente no leite BRPI0805838A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0611536A FR2910786B1 (fr) 2006-12-29 2006-12-29 "procede d'extraction d'une proteine presente dans du lait"
PCT/FR2008/000007 WO2008099077A2 (fr) 2006-12-29 2008-01-02 Procédé d'extraction d'une protéine présente dans du lait

Publications (1)

Publication Number Publication Date
BRPI0805838A2 true BRPI0805838A2 (pt) 2011-08-30

Family

ID=38182268

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0805838-5A BRPI0805838A2 (pt) 2006-12-29 2008-01-02 procedimento para a extração de uma proteìna presente no leite

Country Status (13)

Country Link
US (2) US8492524B2 (pt)
EP (1) EP2134191A2 (pt)
JP (1) JP5196590B2 (pt)
KR (1) KR101294751B1 (pt)
CN (2) CN101600358B (pt)
AR (1) AR064683A1 (pt)
AU (1) AU2008214544B2 (pt)
BR (1) BRPI0805838A2 (pt)
CA (1) CA2673583C (pt)
FR (1) FR2910786B1 (pt)
IL (1) IL199360A (pt)
TW (2) TWI496542B (pt)
WO (1) WO2008099077A2 (pt)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1711513B1 (en) 2003-12-01 2014-07-02 Novo Nordisk Health Care AG Nanofiltration of factor vii solutions to remove virus
FR2901707B1 (fr) 2006-05-31 2017-09-29 Lab Francais Du Fractionnement Composition de facteur vii recombinant ou transgenique, chaque molecule de facteur vii possedant deux sites de n-glycosylation a motifs glycanniques definis
FR2910786B1 (fr) * 2006-12-29 2017-08-11 Laboratoire Francais Du Fractionnement Et Des Biotechnologies (Lfb) "procede d'extraction d'une proteine presente dans du lait"
FR2942232B1 (fr) 2009-02-19 2015-03-13 Lfb Biotechnologies Moyens pour la purification d'une proteine de la coagulation et procedes pour sa mise en oeuvre
FR2942233B1 (fr) * 2009-02-19 2015-03-13 Lfb Biotechnologies Moyens pour la purification d'une proteine du plasma sanguin, et procedes pour sa mise en oeuvre
FR2942231B1 (fr) 2009-02-19 2015-03-20 Lfb Biotechnologies Acides nucleiques se liant specifiquement au facteur vii/viia humain, et utilisations
FR2947181B1 (fr) 2009-06-26 2012-05-04 Lfb Biotechnologies Composition de facteur vii
WO2011112075A1 (en) * 2010-03-11 2011-09-15 N.V. Nutricia Controlling the texture of high-protein nutritional compositions comprising micellar casein
US9364021B2 (en) * 2011-09-09 2016-06-14 Tairob Ltd. Method for preparing micro-foam whipped milk for cappuccino or a method for whipping other liquids containing proteins, using an apparatus
EP2687595B1 (en) * 2012-07-19 2018-05-30 Laboratoire Français du Fractionnement et des Biotechnologies Method for purifying transgenic factor VII
FR3006591B1 (fr) * 2013-06-11 2016-05-06 Lab Francais Du Fractionnement Composition de facteur vii presentant un point isoelectrique substantiellement homogene
US20170295821A1 (en) * 2014-10-06 2017-10-19 Upfront Chromatography A/S Isolation of soluble proteins from aggregated casein-containing mixtures
FR3034419B1 (fr) * 2015-04-02 2017-12-15 Lab Francais Du Fractionnement Procede de purification d'une proteine recombinante therapeutique a partir d'un lait transgenique
US11647737B2 (en) * 2016-12-29 2023-05-16 Asc Therapeutics Inc. Genetically modified rabbit expressing an exogenous protein in milk
FR3076294B1 (fr) 2017-12-29 2022-01-28 Lab Francais Du Fractionnement Procede de purification d'anticorps a partir de lait brut
GB201815053D0 (en) * 2018-09-14 2018-10-31 Dublin Institute Of Tech Method
US12359212B2 (en) 2019-11-22 2025-07-15 Mozza Foods, Inc. Recombinant micelle and method of in vivo assembly
US11326176B2 (en) 2019-11-22 2022-05-10 Mozza Foods, Inc. Recombinant micelle and method of in vivo assembly
CN112159458B (zh) * 2020-09-28 2024-10-29 杭州靡特洛新材料科技有限公司 一种从过期变质牛奶中提取牛奶蛋白的方法
CN118909086B (zh) * 2024-10-12 2025-01-24 安徽天凯生物科技有限公司 一种分离α-乳白蛋白、β-乳球蛋白的方法

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4229342A (en) * 1977-05-18 1980-10-21 Rhone-Poulenc Industries Process for extracting proteins from milk using silica and anion exchange resins
NL8204923A (nl) * 1982-12-21 1984-07-16 Stichting Nl I Zuivelonderzoek Werkwijze voor het bereiden van een precipitaat van caseine en wei-eiwit alsmede aldus bereid precipitaat.
GR860984B (en) 1985-04-17 1986-08-18 Zymogenetics Inc Expression of factor vii and ix activities in mammalian cells
DE122007000007I1 (de) 1986-04-09 2007-05-16 Genzyme Corp Genetisch transformierte Tiere, die ein gewünschtes Protein in Milch absondern
US6984772B1 (en) * 1994-02-18 2006-01-10 Virginia Tech Intellectual Properties, Inc. Transgenic non-human mammals producing fibrinogen in their milk
FR2677652B1 (fr) 1991-06-12 2005-05-27 Agronomique Inst Nat Rech Procede de preparation d'une proteine d'interet dans le lait d'un animal transgenique, produit obtenu et cellule eucaryote utilisee.
US5827690A (en) * 1993-12-20 1998-10-27 Genzyme Transgenics Corporatiion Transgenic production of antibodies in milk
GB9408717D0 (en) * 1994-05-03 1994-06-22 Biotech & Biolog Scien Res DNA sequences
US6713662B1 (en) 1994-07-27 2004-03-30 Pharming Intellectual Property B.V. Production of collagen in the milk of transgenic mammals
US5880327A (en) * 1994-09-21 1999-03-09 American National Red Cross Transgenic mammals expressing human coagulation factor VIII
US6268487B1 (en) * 1996-05-13 2001-07-31 Genzyme Transgenics Corporation Purification of biologically active peptides from milk
US6183803B1 (en) * 1999-06-11 2001-02-06 Biosante Pharmaceuticals, Inc. Method for processing milk
US20040219225A1 (en) * 2001-07-20 2004-11-04 Kivits Marinus Gerardus Cornel Process for obtaining growth factor (tgf-beta and igf-1), lactoperoxidase and immunoglobulins preparations from milk products having low mutual cross-contamination
US20040117862A1 (en) * 2002-03-11 2004-06-17 Cooper Julian D. Production of high levels of transgenic factor VII with engineered stability and its therapeutic uses
AU2003253625A1 (en) * 2002-06-05 2004-04-08 Henry C. Rust Injection molding head
FR2841747B1 (fr) * 2002-07-02 2004-08-20 Cie Laitiere Europeenne Isolat de proteines de lait et procede pour sa preparation
US6911323B2 (en) * 2002-09-25 2005-06-28 Novo Nordisk Healthcare A/G Human coagulation factor VII polypeptides
JP4247880B2 (ja) * 2002-12-24 2009-04-02 Ｔｄｋ株式会社 電子部品の製造方法
WO2004076695A1 (en) 2003-02-24 2004-09-10 Gtc Biotherapeutics, Inc. Methods of tangential flow filtration and an apparatus therefore
WO2005089040A2 (en) * 2004-03-23 2005-09-29 Chr. Hansen A/S Method of producing chymosin c and uses thereof
US20050272917A1 (en) * 2004-05-14 2005-12-08 Hematech, Llc Methods for immunoglobulin purification
EP1869067A1 (en) * 2005-04-11 2007-12-26 Medarex, Inc. Protein purification using hcic amd ion exchange chromatography
FR2910786B1 (fr) * 2006-12-29 2017-08-11 Laboratoire Francais Du Fractionnement Et Des Biotechnologies (Lfb) "procede d'extraction d'une proteine presente dans du lait"

Also Published As

Publication number Publication date
IL199360A0 (en) 2010-03-28
AR064683A1 (es) 2009-04-22
CN101600358B (zh) 2014-05-07
CA2673583C (en) 2013-06-11
AU2008214544A1 (en) 2008-08-21
CN101600358A (zh) 2009-12-09
US20090281283A1 (en) 2009-11-12
TW201350024A (zh) 2013-12-16
EP2134191A2 (fr) 2009-12-23
WO2008099077A3 (fr) 2009-05-07
CN103910778A (zh) 2014-07-09
JP2010514746A (ja) 2010-05-06
US8492524B2 (en) 2013-07-23
TW200838437A (en) 2008-10-01
FR2910786B1 (fr) 2017-08-11
US20130295646A1 (en) 2013-11-07
AU2008214544B2 (en) 2013-06-20
KR101294751B1 (ko) 2013-08-12
TWI519239B (zh) 2016-02-01
IL199360A (en) 2013-05-30
CA2673583A1 (en) 2008-08-21
JP5196590B2 (ja) 2013-05-15
WO2008099077A2 (fr) 2008-08-21
TWI496542B (zh) 2015-08-21
FR2910786A1 (fr) 2008-07-04
KR20090113825A (ko) 2009-11-02

Similar Documents

Publication Publication Date Title
BRPI0805838A2 (pt) procedimento para a extração de uma proteìna presente no leite
Gevaert et al. Reversible labeling of cysteine‐containing peptides allows their specific chromatographic isolation for non‐gel proteome studies
Govender et al. A novel and more efficient biosynthesis approach for human insulin production in Escherichia coli (E. coli)
Li et al. Cadmium-binding proteins in human blood plasma
WO2003059935A3 (en) Methods for purifying protein
BR112014015101A8 (pt) métodos para intensificar eficiência de etapas de cromatografia a jusante para purificação de anticorpos
NO20060236L (no) Antistoffer spesifikke for sklerostinro og fremgangsmate for a oke benmineralisering
BRPI0606790A2 (pt) anticorpo humano isolado ou porção de ligação de antìgeno do mesmo, polipeptìdeo isolado, composição, ácido nucleico isolado, vetor de expressão célula hospedeira, vacina contra a raiva, kit, uso de um anticorpo monoclonal humano isolado ou porção de ligação de antìgeno do mesmo, ou de uma quantidade eficaz de uma composição, método para identificar um anticorpo ou um fragmento do mesmo, anticorpo ou fragmento do mesmo, anticorpo ou fragmento do mesmo,e, glicoproteìna.
BRPI0416544A (pt) proteìna de ligação de gag
Fountoulakis et al. Enrichment and purification of proteins of Haemophilus influenzae by chromatofocusing
BR0210386A (pt) Purificação de albumina de soro humano
WO2004024182A3 (en) Method for isolating hepatitis c virus peptides
Collatz et al. Group fractionation of eukaryotic ribosomal proteins.
González-Ortega et al. Production and purification of recombinant hypocholesterolemic peptides
Fedosov et al. Transcobalamin from cow milk: isolation and physico-chemical properties
SE0200543D0 (sv) Method of separation using aromatic thioether ligands
Murayama et al. Estrogen Receptor of Cow Uterus: III. Molecular Constitution of Estrogen Receptor-Binding Factors
Labonté et al. The relative ligand binding preference of the murine ileal lipid binding protein
Snyder et al. The nature of the antibody in iso-immune anti-L sera affecting active potassium transport in LK sheep red cells
Büsen The subunit structure of calf thymus ribonuclease H i as revealed by immunological analysis.
Haro et al. Divalent metal cation chelators enhance chromatographic separation of structurally similar macromolecules: separation of human growth hormone isoforms
Stratakis et al. Purification and properties of the fatty acid‐binding VHDL from the hemolymph of the spider Eurypelma californicum
Chen et al. Characterization of galanin receptor in canine small intestinal circular muscle synaptosomes
Haino et al. Purification of a 60 kDa nuclear localization signal binding protein in rat liver nuclear envelopes and characterization of its properties
Dall’olio et al. Affinity chromatography of ovine casein

Legal Events

Date Code Title Description
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 11A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2495 DE 30-10-2018 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.