WO2008099077A3 - Procédé d'extraction d'une protéine présente dans du lait - Google Patents

Procédé d'extraction d'une protéine présente dans du lait Download PDF

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Publication number
WO2008099077A3
WO2008099077A3 PCT/FR2008/000007 FR2008000007W WO2008099077A3 WO 2008099077 A3 WO2008099077 A3 WO 2008099077A3 FR 2008000007 W FR2008000007 W FR 2008000007W WO 2008099077 A3 WO2008099077 A3 WO 2008099077A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
milk
extracting
fraction
substrate
Prior art date
Application number
PCT/FR2008/000007
Other languages
English (en)
Other versions
WO2008099077A2 (fr
Inventor
Michel Nogre
Alain Lejars
Monique Ollivier
Original Assignee
Lfb Biotechnologies
Michel Nogre
Alain Lejars
Monique Ollivier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU2008214544A priority Critical patent/AU2008214544B2/en
Priority to EP08761732A priority patent/EP2134191A2/fr
Priority to BRPI0805838-5A priority patent/BRPI0805838A2/pt
Priority to CN200880001548.0A priority patent/CN101600358B/zh
Priority to CA2673583A priority patent/CA2673583C/fr
Priority to US12/520,826 priority patent/US8492524B2/en
Application filed by Lfb Biotechnologies, Michel Nogre, Alain Lejars, Monique Ollivier filed Critical Lfb Biotechnologies
Priority to JP2009543512A priority patent/JP5196590B2/ja
Priority to KR1020097013591A priority patent/KR101294751B1/ko
Publication of WO2008099077A2 publication Critical patent/WO2008099077A2/fr
Publication of WO2008099077A3 publication Critical patent/WO2008099077A3/fr
Priority to IL199360A priority patent/IL199360A/en
Priority to US13/910,517 priority patent/US20130295646A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Public Health (AREA)
  • Microbiology (AREA)
  • Transplantation (AREA)
  • Reproductive Health (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Dairy Products (AREA)

Abstract

L'invention concerne un procédé d'extraction d'une protéine présente dans du lait, présentant au moins une poche hydrophobe et une charge négative au pH naturel du lait, comprenant les étapes suivantes de: a) Ecrémage et délipidation dudit lait, b) Passage de la fraction délipidée et écrémée contenant ladite protéine sur un support chromatographique sur lequel est greffé un ligand présentant à la fois un caractère hydrophobe et un caractère ionique, dans des conditions de pH permettant à ladite protéine d'être retenue sur ledit support, le pH étant supérieur à 4,6, c) Elution de la protéine, d) Purification de la fraction éluée par élimination des protéines du lait de ladite fraction éluée, et e) Récupération de ladite protéine.
PCT/FR2008/000007 2006-12-29 2008-01-02 Procédé d'extraction d'une protéine présente dans du lait WO2008099077A2 (fr)

Priority Applications (10)

Application Number Priority Date Filing Date Title
EP08761732A EP2134191A2 (fr) 2006-12-29 2008-01-02 Procédé d'extraction d'une protéine présente dans du lait
BRPI0805838-5A BRPI0805838A2 (pt) 2006-12-29 2008-01-02 procedimento para a extração de uma proteìna presente no leite
CN200880001548.0A CN101600358B (zh) 2006-12-29 2008-01-02 从乳中提取蛋白质的方法
CA2673583A CA2673583C (fr) 2006-12-29 2008-01-02 Procede d'extraction d'une proteine presente dans du lait
US12/520,826 US8492524B2 (en) 2006-12-29 2008-01-02 Method for extracting a protein from milk
AU2008214544A AU2008214544B2 (en) 2006-12-29 2008-01-02 Method for extracting a protein from milk
JP2009543512A JP5196590B2 (ja) 2006-12-29 2008-01-02 ミルクからの因子ｖｉｉの抽出方法
KR1020097013591A KR101294751B1 (ko) 2006-12-29 2008-01-02 우유에서 단백질을 추출하는 방법
IL199360A IL199360A (en) 2006-12-29 2009-06-15 A method for extracting protein from milk
US13/910,517 US20130295646A1 (en) 2006-12-29 2013-06-05 Method for extracting a protein from milk

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0611536 2006-12-29
FR0611536A FR2910786B1 (fr) 2006-12-29 2006-12-29 "procede d'extraction d'une proteine presente dans du lait"

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/910,517 Continuation US20130295646A1 (en) 2006-12-29 2013-06-05 Method for extracting a protein from milk

Publications (2)

Publication Number Publication Date
WO2008099077A2 WO2008099077A2 (fr) 2008-08-21
WO2008099077A3 true WO2008099077A3 (fr) 2009-05-07

Family

ID=38182268

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2008/000007 WO2008099077A2 (fr) 2006-12-29 2008-01-02 Procédé d'extraction d'une protéine présente dans du lait

Country Status (13)

Country Link
US (2) US8492524B2 (fr)
EP (1) EP2134191A2 (fr)
JP (1) JP5196590B2 (fr)
KR (1) KR101294751B1 (fr)
CN (2) CN101600358B (fr)
AR (1) AR064683A1 (fr)
AU (1) AU2008214544B2 (fr)
BR (1) BRPI0805838A2 (fr)
CA (1) CA2673583C (fr)
FR (1) FR2910786B1 (fr)
IL (1) IL199360A (fr)
TW (2) TWI519239B (fr)
WO (1) WO2008099077A2 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1711513E (pt) 2003-12-01 2014-10-02 Novo Nordisk Healthcare Ag Nanofiltração de soluções de fator vii para remover vírus
FR2901707B1 (fr) 2006-05-31 2017-09-29 Lab Francais Du Fractionnement Composition de facteur vii recombinant ou transgenique, chaque molecule de facteur vii possedant deux sites de n-glycosylation a motifs glycanniques definis
FR2910786B1 (fr) * 2006-12-29 2017-08-11 Laboratoire Francais Du Fractionnement Et Des Biotechnologies (Lfb) "procede d'extraction d'une proteine presente dans du lait"
FR2942232B1 (fr) * 2009-02-19 2015-03-13 Lfb Biotechnologies Moyens pour la purification d'une proteine de la coagulation et procedes pour sa mise en oeuvre
FR2942231B1 (fr) 2009-02-19 2015-03-20 Lfb Biotechnologies Acides nucleiques se liant specifiquement au facteur vii/viia humain, et utilisations
FR2942233B1 (fr) * 2009-02-19 2015-03-13 Lfb Biotechnologies Moyens pour la purification d'une proteine du plasma sanguin, et procedes pour sa mise en oeuvre
FR2947181B1 (fr) 2009-06-26 2012-05-04 Lfb Biotechnologies Composition de facteur vii
WO2011112075A1 (fr) * 2010-03-11 2011-09-15 N.V. Nutricia Régulation de la texture de compositions nutritionnelles à forte teneur en protéines contenant de la caséine micellaire
US9364021B2 (en) * 2011-09-09 2016-06-14 Tairob Ltd. Method for preparing micro-foam whipped milk for cappuccino or a method for whipping other liquids containing proteins, using an apparatus
EP2687595B1 (fr) * 2012-07-19 2018-05-30 Laboratoire Français du Fractionnement et des Biotechnologies Procédé de purification de facteur VII transgénique
FR3006591B1 (fr) 2013-06-11 2016-05-06 Lab Francais Du Fractionnement Composition de facteur vii presentant un point isoelectrique substantiellement homogene
WO2016055064A2 (fr) * 2014-10-06 2016-04-14 Upfront Chromatography A/S Isolation de protéines solubles à partir de mélanges contenant de la caséine agrégée
FR3034419B1 (fr) * 2015-04-02 2017-12-15 Lab Francais Du Fractionnement Procede de purification d'une proteine recombinante therapeutique a partir d'un lait transgenique
WO2018126095A1 (fr) * 2016-12-29 2018-07-05 Applied Stemcell, Inc. Animaux transgéniques et méthode de bioproduction
FR3076294B1 (fr) 2017-12-29 2022-01-28 Lab Francais Du Fractionnement Procede de purification d'anticorps a partir de lait brut
GB201815053D0 (en) * 2018-09-14 2018-10-31 Dublin Institute Of Tech Method
US11326176B2 (en) 2019-11-22 2022-05-10 Mozza Foods, Inc. Recombinant micelle and method of in vivo assembly
CN112159458A (zh) * 2020-09-28 2021-01-01 靡特洛 一种从过期变质牛奶中提取牛奶蛋白的方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4229342A (en) * 1977-05-18 1980-10-21 Rhone-Poulenc Industries Process for extracting proteins from milk using silica and anion exchange resins
US6183803B1 (en) * 1999-06-11 2001-02-06 Biosante Pharmaceuticals, Inc. Method for processing milk
FR2841747A1 (fr) * 2002-07-02 2004-01-09 Cie Laitiere Europeenne Isolat de proteines de lait et procede pour sa preparation
US20040219225A1 (en) * 2001-07-20 2004-11-04 Kivits Marinus Gerardus Cornel Process for obtaining growth factor (tgf-beta and igf-1), lactoperoxidase and immunoglobulins preparations from milk products having low mutual cross-contamination
WO2005089040A2 (fr) * 2004-03-23 2005-09-29 Chr. Hansen A/S Procede de production de la chymosine c et ses utilisations
US20050272917A1 (en) * 2004-05-14 2005-12-08 Hematech, Llc Methods for immunoglobulin purification

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Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4229342A (en) * 1977-05-18 1980-10-21 Rhone-Poulenc Industries Process for extracting proteins from milk using silica and anion exchange resins
US6183803B1 (en) * 1999-06-11 2001-02-06 Biosante Pharmaceuticals, Inc. Method for processing milk
US20040219225A1 (en) * 2001-07-20 2004-11-04 Kivits Marinus Gerardus Cornel Process for obtaining growth factor (tgf-beta and igf-1), lactoperoxidase and immunoglobulins preparations from milk products having low mutual cross-contamination
FR2841747A1 (fr) * 2002-07-02 2004-01-09 Cie Laitiere Europeenne Isolat de proteines de lait et procede pour sa preparation
WO2005089040A2 (fr) * 2004-03-23 2005-09-29 Chr. Hansen A/S Procede de production de la chymosine c et ses utilisations
US20050272917A1 (en) * 2004-05-14 2005-12-08 Hematech, Llc Methods for immunoglobulin purification

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Title
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Also Published As

Publication number Publication date
AU2008214544A1 (en) 2008-08-21
US20090281283A1 (en) 2009-11-12
CN101600358B (zh) 2014-05-07
AR064683A1 (es) 2009-04-22
CN103910778A (zh) 2014-07-09
FR2910786A1 (fr) 2008-07-04
KR20090113825A (ko) 2009-11-02
CN101600358A (zh) 2009-12-09
EP2134191A2 (fr) 2009-12-23
WO2008099077A2 (fr) 2008-08-21
US8492524B2 (en) 2013-07-23
US20130295646A1 (en) 2013-11-07
AU2008214544B2 (en) 2013-06-20
IL199360A (en) 2013-05-30
TW201350024A (zh) 2013-12-16
CA2673583C (fr) 2013-06-11
BRPI0805838A2 (pt) 2011-08-30
JP5196590B2 (ja) 2013-05-15
CA2673583A1 (fr) 2008-08-21
IL199360A0 (en) 2010-03-28
TWI519239B (zh) 2016-02-01
TW200838437A (en) 2008-10-01
JP2010514746A (ja) 2010-05-06
TWI496542B (zh) 2015-08-21
FR2910786B1 (fr) 2017-08-11
KR101294751B1 (ko) 2013-08-12

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