WO2009063069A3 - Protein purification and endotoxin removal - Google Patents

Protein purification and endotoxin removal Download PDF

Info

Publication number
WO2009063069A3
WO2009063069A3 PCT/EP2008/065595 EP2008065595W WO2009063069A3 WO 2009063069 A3 WO2009063069 A3 WO 2009063069A3 EP 2008065595 W EP2008065595 W EP 2008065595W WO 2009063069 A3 WO2009063069 A3 WO 2009063069A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein purification
endotoxin removal
endotoxin
removal
reagents
Prior art date
Application number
PCT/EP2008/065595
Other languages
French (fr)
Other versions
WO2009063069A2 (en
Inventor
Rikke Christina Nielsen
Original Assignee
Novo Nordisk A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk A/S filed Critical Novo Nordisk A/S
Priority to EP08849938A priority Critical patent/EP2220108A2/en
Publication of WO2009063069A2 publication Critical patent/WO2009063069A2/en
Publication of WO2009063069A3 publication Critical patent/WO2009063069A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/22—Hormones
    • A61K38/2257—Prolactin

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

This invention relates to a process for purifying peptides, in particular but not exclusively, to a process for removing endotoxins from a peptide solution, to a kit comprising reagents for said process and to the purified peptide obtained by said process.
PCT/EP2008/065595 2007-11-15 2008-11-14 Endotoxin removal WO2009063069A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP08849938A EP2220108A2 (en) 2007-11-15 2008-11-14 Protein purification and endotoxin removal

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07022183.3 2007-11-15
EP07022183 2007-11-15

Publications (2)

Publication Number Publication Date
WO2009063069A2 WO2009063069A2 (en) 2009-05-22
WO2009063069A3 true WO2009063069A3 (en) 2010-02-25

Family

ID=40568529

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/065595 WO2009063069A2 (en) 2007-11-15 2008-11-14 Endotoxin removal

Country Status (2)

Country Link
EP (1) EP2220108A2 (en)
WO (1) WO2009063069A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012500250A (en) 2008-08-21 2012-01-05 オクタファルマ アクチェン ゲゼルシャフト Recombinantly produced human factor VIII and factor IX
ES2670827T3 (en) * 2010-03-30 2018-06-01 Octapharma Ag Process for purification of granulocyte colony stimulating factor, G-CSF
SG191186A1 (en) 2010-12-15 2013-07-31 Baxter Int Eluate collection using conductivity gradient

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5153265A (en) * 1988-01-20 1992-10-06 Cetus Corporation Conjugation of polymer to colony stimulating factor-1
WO1999063076A1 (en) * 1998-06-01 1999-12-09 The Immune Response Corporation Novel method of large scale plasmid purification

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5153265A (en) * 1988-01-20 1992-10-06 Cetus Corporation Conjugation of polymer to colony stimulating factor-1
WO1999063076A1 (en) * 1998-06-01 1999-12-09 The Immune Response Corporation Novel method of large scale plasmid purification

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANSPACH F B ET AL: "Removal of endotoxins by affinity sorbents", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, NL, vol. 711, no. 1, 8 September 1995 (1995-09-08), pages 81 - 92, XP004038953, ISSN: 0021-9673 *
NEIDHARDT E A ET AL: "Rapid, two-step purification process for the preparation of pyrogen-free murine immunoglobulin G1 monoclonal antibodies", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, NL, vol. 590, no. 2, 31 January 1992 (1992-01-31), pages 255 - 261, XP026538910, ISSN: 0021-9673, [retrieved on 19920131] *

Also Published As

Publication number Publication date
WO2009063069A2 (en) 2009-05-22
EP2220108A2 (en) 2010-08-25

Similar Documents

Publication Publication Date Title
WO2008087184A3 (en) Process for the purification of fc-containing proteins
IL187642A0 (en) METHODS FOR PURIFYING PROTEINS HAVING Fc REGIONS
ZA200900836B (en) Process for the purification of FC-fusion proteins
HK1131400A1 (en) Process for the purification of fc-containing proteins
EA200702210A1 (en) METHOD FOR CLEANING PROTEINS
EA201201132A1 (en) CLEANING ANTIBODIES WITH THE HELP OF A SINGLE FUNCTIONAL UNIT
WO2007038363A3 (en) Utilizing ionic liquids for hydrofluorocarbon separation
ZA201203825B (en) Speicific sorbent for binding proteins and peptides, and separation method using the same
WO2003066662A3 (en) Protein purification
WO2007019515A3 (en) Method for enabling the provision of purified carbon dioxide
DE602004006157D1 (en) PROCESS FOR PURIFYING IL-18 BINDING PROTEIN
ATE489156T1 (en) GAS PURIFICATION SYSTEM
DE602005009827D1 (en) PROCESS FOR PURIFYING IL-18 BINDING PROTEIN
WO2010151688A3 (en) Capture purification processes for proteins expressed in a non-mammalian system
MY150516A (en) Contaminant removal from a gas stream
RU2016104651A (en) PROTEASIS RESISTANT PEPTIDE LIGANDS
DE502006002897D1 (en) PROCESS FOR CLEANING G-CSF
WO2008051178A3 (en) Purification of proteins with cationic surfactant
IL204949A0 (en) Method for purifying an fc- containing protein
EP1869230A4 (en) Method for purifying proteins
WO2009063069A3 (en) Protein purification and endotoxin removal
PL1960521T3 (en) Method and test kit for the separation, purification and recovery of long- and short-chain nucleic acids
DK1989189T3 (en) Adsorbents for protein purification
ATE417056T1 (en) PROTEIN PURIFICATION PROCESS
PL1977653T3 (en) Method for gathering vegetable proteins and/or peptides, proteins and/or peptides produced through this method and usage thereof

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08849938

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2008849938

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: DE