AR061734A1 - Rendimiento mejorado y tolerancia al estres en plantas transgenicas - Google Patents

Rendimiento mejorado y tolerancia al estres en plantas transgenicas

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Publication number
AR061734A1
AR061734A1 ARP070102904A ARP070102904A AR061734A1 AR 061734 A1 AR061734 A1 AR 061734A1 AR P070102904 A ARP070102904 A AR P070102904A AR P070102904 A ARP070102904 A AR P070102904A AR 061734 A1 AR061734 A1 AR 061734A1
Authority
AR
Argentina
Prior art keywords
plant
tolerance
transgenic
transgenic plant
polypeptide
Prior art date
Application number
ARP070102904A
Other languages
English (en)
Inventor
Robert Meister
Robert A Creelman
Neal I Gutterson
Oliver J Ratcliffe
Marie Petracek
Kimberly F Duff
Thomas Ruff
Eric R Cerny
Susanne Kjemtrup-Lovelace
T Lynne Reuber
Qinzhang Xu
Original Assignee
Mendel Biotechnology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mendel Biotechnology Inc filed Critical Mendel Biotechnology Inc
Publication of AR061734A1 publication Critical patent/AR061734A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se introdujeron polinucleotidos y polipéptidos incorporados en vectores de expresion, de plantas, y se expresaron ectopicamente. Los polipéptidos de la presente han demostrado conferir al menos una actividad regulatoria y confieren mayor rendimiento, mayor altura, mayor crecimiento estacional temprano, mayor cubrimiento de área, mayor diámetro de tallo, mayor vigor estacional temprano, más raíces secundarias, germinacion más rápida, mayor tolerancia al frío, mayor tolerancia a la falta de agua, menor conductancia por estomas, sensado C/N alterado, mayor tolerancia a bajo nitrogeno, mayor tolerancia a bajo fosforo, o mayor tolerancia a estrés hiperosmotico comparado con la planta control. Reivindicacion 1: Un método para producir una planta transgénica que tenga una característica alterada en relacion a una planta control, caracterizado porque comprende los pasos de: (a) proveer un vector de expresion que codifica un polipéptido que comprende un dominio conservado de dedos de zinc caja B que comparte una identidad de aminoácidos con una de SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56 o 66; en donde, cuando se sobreexpresa el polipéptido en una planta, el polipéptido regula la transcripcion y confiere al menos una actividad regulatoria que resulta en la característica alterada en relacion a una planta control; en donde el porcentaje de identidad de aminoácidos se elige del grupo que consiste en al menos alrededor de 56%, al menos alrededor de 58%, al menos alrededor de 60% de identidad de secuencia, al menos alrededor de 65%, al menos alrededor de 67%, al menos alrededor de 70%, al menos alrededor de 75%, al menos alrededor de 76%, al menos alrededor de 77%, al menos alrededor de 78%, al menos alrededor de 79%, al menos alrededor de 80%, al menos alrededor de 81%, al menos alrededor de 82%, al menos alrededor de 83%, al menos alrededor de 84%, al menos alrededor de 85%, al menos alrededor de 86%, al menos alrededor de 87%, al menos alrededor de 88%, al menos alrededor de 89%, al menos alrededor de 90%, al menos alrededor de 91%, al menos alrededor de 92%, al menos alrededor de 93%, al menos alrededor de 94%, al menos alrededor de 95%, al menos alrededor de 96%, al menos alrededor de 97%, al menos alrededor de 98%, al menos alrededor de 99%, y alrededor de 100%; y (b) introducir el vector de expresion en una planta objetivo para producir la planta transgénica; en donde la característica alterada se elige del grupo que consiste en mayor crecimiento estacional temprano, mayor crecimiento estacional tardío y vigor, mayor cubrimiento de área, mayor altura, mayor diámetro de tallo, mayor resistencia al almacenamiento, mayor longitud internodal, más raíces secundarias, mayor tolerancia al frío, mayor tolerancia a la falta de agua, menor conductancia por estomas, mayor tolerancia a estrés hiperosmotico, y mayor numero de nodos de tallo principal; y (c) opcionalmente, identificar la planta transgénica por medio de la seleccion de la planta transgénica que sobreexpresa el polipéptido o por medio de la seleccion de la planta transgénica que tiene una característica alterada en relacion a la planta control. Reivindicacion 9: Una semilla transgénica que se produjo a partir de la planta transgénica que se produjo en el método de la reivindicacion 1, caracterizada porque la semilla transgénica comprende el vector de expresion de la reivindicacion 1.
ARP070102904A 2006-06-29 2007-06-28 Rendimiento mejorado y tolerancia al estres en plantas transgenicas AR061734A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US81788606P 2006-06-29 2006-06-29

Publications (1)

Publication Number Publication Date
AR061734A1 true AR061734A1 (es) 2008-09-17

Family

ID=38895082

Family Applications (3)

Application Number Title Priority Date Filing Date
ARP070102904A AR061734A1 (es) 2006-06-29 2007-06-28 Rendimiento mejorado y tolerancia al estres en plantas transgenicas
ARP180102738A AR113137A2 (es) 2006-06-29 2018-09-24 Un método para producir una planta de soja transgénica que tenga una característica alterada en relación a una planta de soja control, polinucleótido recombinante, construcción de expresión aislada y célula aislada de planta de soja
ARP180103728A AR113985A2 (es) 2006-06-29 2018-12-19 Rendimiento mejorado y tolerancia al estrés en plantas transgénicas

Family Applications After (2)

Application Number Title Priority Date Filing Date
ARP180102738A AR113137A2 (es) 2006-06-29 2018-09-24 Un método para producir una planta de soja transgénica que tenga una característica alterada en relación a una planta de soja control, polinucleótido recombinante, construcción de expresión aislada y célula aislada de planta de soja
ARP180103728A AR113985A2 (es) 2006-06-29 2018-12-19 Rendimiento mejorado y tolerancia al estrés en plantas transgénicas

Country Status (8)

Country Link
EP (4) EP2912938B1 (es)
CN (2) CN105734076A (es)
AR (3) AR061734A1 (es)
BR (1) BRPI0713794B1 (es)
CA (4) CA2655870C (es)
MX (1) MX2008016224A (es)
WO (1) WO2008005210A2 (es)
ZA (1) ZA200900024B (es)

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ZA200900024B (en) * 2006-06-29 2010-04-28 Mendel Biotechnology Inc Improved yield and stress tolerance in transgenic plants
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CN103172717B (zh) * 2013-03-01 2014-09-17 中国农业科学院油料作物研究所 植物耐低钾胁迫相关蛋白GmWRKY50及其编码基因与应用
CN103646193B (zh) * 2013-12-24 2016-07-06 辽宁大学 一种用于近缘物种鉴别的pcr引物设计方法
CN105255941B (zh) * 2015-11-27 2018-09-21 山东省水稻研究所 基因OsBBX14在提高水稻干旱胁迫耐性中的应用
CN107435044B (zh) * 2016-05-25 2020-11-20 北京大学 水稻雄蕊特异表达的启动子及其应用
CN109234305B (zh) * 2018-07-16 2021-08-03 浙江理工大学 一种棉花性状改良的方法
CN110504006B (zh) * 2019-07-15 2023-03-28 广州奇辉生物科技有限公司 一种处理扩增子数据的方法、系统、平台及存储介质
CN110904110B (zh) * 2019-12-18 2021-04-23 四川省农业科学院生物技术核技术研究所 降低OsHAP3C基因表达在培育抽穗期推后、生育期延长水稻品种中的应用
CN116064653B (zh) * 2022-09-15 2024-05-14 浙江大学 番茄SlBBX17基因在促进番茄低温抗性中的应用

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Also Published As

Publication number Publication date
CA2918565A1 (en) 2008-01-10
ZA200900024B (en) 2010-04-28
EP2912938B1 (en) 2019-05-29
EP2040533A4 (en) 2009-12-16
CA2655870C (en) 2016-04-05
CA2995933C (en) 2020-07-07
WO2008005210A3 (en) 2008-12-11
AR113137A2 (es) 2020-01-29
CA3080579A1 (en) 2008-01-10
EP2040533A2 (en) 2009-04-01
EP2500433A3 (en) 2013-02-20
CA2655870A1 (en) 2008-01-10
CN101478870B (zh) 2016-03-02
CN101478870A (zh) 2009-07-08
EP3574747A1 (en) 2019-12-04
EP2040533B1 (en) 2015-05-20
WO2008005210A2 (en) 2008-01-10
CA2995933A1 (en) 2008-01-10
CN105734076A (zh) 2016-07-06
EP2500433A2 (en) 2012-09-19
BRPI0713794B1 (pt) 2018-05-29
MX2008016224A (es) 2009-02-23
AR113985A2 (es) 2020-07-08
BRPI0713794A2 (pt) 2012-11-06
CA2918565C (en) 2018-04-10
EP2912938A1 (en) 2015-09-02

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