AR033878A1 - Clonacion y secuenciamiento de genes de piruvato descarboxilasa (pdc) de bacterias y usos de los mismos - Google Patents

Clonacion y secuenciamiento de genes de piruvato descarboxilasa (pdc) de bacterias y usos de los mismos

Info

Publication number
AR033878A1
AR033878A1 ARP020101630A ARP020101630A AR033878A1 AR 033878 A1 AR033878 A1 AR 033878A1 AR P020101630 A ARP020101630 A AR P020101630A AR P020101630 A ARP020101630 A AR P020101630A AR 033878 A1 AR033878 A1 AR 033878A1
Authority
AR
Argentina
Prior art keywords
host cells
descarboxylase
piruvato
clonation
pdc
Prior art date
Application number
ARP020101630A
Other languages
English (en)
Original Assignee
Univ Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida filed Critical Univ Florida
Publication of AR033878A1 publication Critical patent/AR033878A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La invención provee moléculas de ácidos nucleicos aislados que codifican enzimas piruvato descarboxilasa que tienen actividad de descarboxilasa, afinidad con el sustrato, termoestabilidad y actividad a diferentes pH mejoradas. Los ácidos nucleicos de la invención también tienen un uso de codon que permite la alta expresión en una variedad de células huéspedes. Por consiguiente, la invención provee vectores de expresión recombinantes que contienen tales moléculas de ácidos nucleicos, células huéspedes recombinantes que comprenden los vectores de expresión, células huéspedes que comprenden otras enzimas etanológenas, y métodos para producir sustancias útiles, por ejemplo, acetaldehído y etanol utilizando tales células huéspedes.
ARP020101630A 2001-05-04 2002-05-03 Clonacion y secuenciamiento de genes de piruvato descarboxilasa (pdc) de bacterias y usos de los mismos AR033878A1 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US28863801P 2001-05-04 2001-05-04
US28867101P 2001-05-04 2001-05-04
US28869901P 2001-05-04 2001-05-04
US28869801P 2001-05-04 2001-05-04
US28862201P 2001-05-04 2001-05-04

Publications (1)

Publication Number Publication Date
AR033878A1 true AR033878A1 (es) 2004-01-07

Family

ID=27540714

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP020101630A AR033878A1 (es) 2001-05-04 2002-05-03 Clonacion y secuenciamiento de genes de piruvato descarboxilasa (pdc) de bacterias y usos de los mismos

Country Status (12)

Country Link
US (2) US7326551B2 (es)
EP (1) EP1527164A4 (es)
JP (3) JP2005508629A (es)
KR (1) KR100708794B1 (es)
CN (1) CN1678735A (es)
AR (1) AR033878A1 (es)
AU (2) AU2002353763B2 (es)
BR (1) BR0209425A (es)
CA (1) CA2445205A1 (es)
MX (1) MXPA03009877A (es)
NZ (1) NZ529910A (es)
WO (1) WO2003025117A2 (es)

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US20020039776A1 (en) * 2000-06-09 2002-04-04 Thomas Jenuwein Mammalian SUV39H2 proteins and isolated DNA molecules encoding them
CN1436239B (zh) 2000-06-16 2010-04-28 财团法人地球环境产业技术研究机构 用重组棒状杆菌生产乙醇的方法
EP1636246B1 (en) * 2003-06-24 2015-08-19 Scientist of Fortune S.A. Production of 2 -deoxynucleosides and 2 -deoxynucleoside precursors from 2-dehydro-3-deoxy-d-gluconate
JP2008527985A (ja) * 2005-01-24 2008-07-31 ディーエスエム アイピー アセッツ ビー．ブイ． 糸状菌細胞において目的の化合物を産生させるための方法
WO2007084477A1 (en) * 2006-01-13 2007-07-26 University Of Hawaii Methods and compositions for ethanol producing cyanobacteria
EP2546349A1 (en) 2007-04-03 2013-01-16 Tabletop Energy Methods for transforming eukaryotic algae
US20090246841A1 (en) * 2008-03-26 2009-10-01 Jamieson Andrew C Methods and compositions for production of acetaldehyde
JP2010017094A (ja) * 2008-07-08 2010-01-28 Thermostable Enzyme Laboratory Co Ltd 酵素反応によりアセトアルデヒドを製造する方法
WO2010062707A1 (en) * 2008-10-30 2010-06-03 Joule Unlimited, Inc. Methods and compositions for producing carbon-based products of interest in micro-organisms
CN102317464B (zh) * 2008-12-12 2014-10-29 塞莱西翁有限公司 从α-酮酸生物合成双官能烷烃
BR112013023592A2 (pt) 2011-03-14 2017-06-06 Easel Biotechnologies Llc síntese microbiana de aldeídos e alcoóis correspondentes
KR101796983B1 (ko) 2011-11-03 2017-11-13 이젤 바이오테크날러지스, 엘엘씨 미생물에 의한 ｎ-부티르알데하이드의 생성
US20130288320A1 (en) 2012-04-27 2013-10-31 Bioamber Inc. Methods and microorganisms for increasing the biological synthesis of difunctional alkanes
GB201209041D0 (en) 2012-05-22 2012-07-04 Imp Innovations Ltd Protein manipulation
CA3212772A1 (en) 2012-11-23 2014-05-30 Seres Therapeutics, Inc. Synergistic bacterial compositions and methods of production and use thereof
US8906668B2 (en) 2012-11-23 2014-12-09 Seres Health, Inc. Synergistic bacterial compositions and methods of production and use thereof
EP3584308A3 (en) 2013-02-04 2020-03-04 Seres Therapeutics, Inc. Compositions and methods
EP2951283A4 (en) 2013-02-04 2017-01-25 Seres Therapeutics, Inc. Compositions and methods
WO2014145958A2 (en) 2013-03-15 2014-09-18 Seres Health, Inc. Network-based microbial compositions and methods
JP2016165225A (ja) 2013-07-09 2016-09-15 味の素株式会社 有用物質の製造方法
RU2722482C2 (ru) 2013-11-25 2020-06-01 Серес Терапеутикс, Инк. Синергические бактериальные композиции и способы их получения и применения
US20170087196A1 (en) 2014-05-19 2017-03-30 Memorial Sloan-Kettering Cancer Center Methods and compositions for reducing clostridium difficile infection
JP5947470B2 (ja) * 2014-05-29 2016-07-06 長瀬産業株式会社 微生物の物質生産性を向上させる方法および該方法に用いるキット
JP2020530840A (ja) 2017-08-14 2020-10-29 セレス セラピューティクス インコーポレイテッド 胆汁うっ滞性疾患を治療するための組成物及び方法
CN108315365B (zh) * 2018-02-08 2021-01-29 浙江宏元药业股份有限公司 阿托伐他汀中间体的生物合成方法
CN110540952B (zh) * 2019-09-09 2021-05-11 北京科技大学 产叶黄素原核微生物及其在生产叶黄素中的应用
WO2021142019A1 (en) * 2020-01-06 2021-07-15 Solugen, Inc. Compositions, systems and methods for production of value-added chemicals
CN112063638B (zh) * 2020-07-14 2022-03-18 深圳大学 一种从秋茄中克隆扩增出来的丙酮酸脱羧酶基因及其应用
WO2022217117A1 (en) * 2021-04-08 2022-10-13 University Of Florida Research Foundation, Incorporated Enzymatic synthesis of mycosporine-like amino acids

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Also Published As

Publication number Publication date
WO2003025117A3 (en) 2005-02-10
EP1527164A4 (en) 2006-02-01
JP2008099704A (ja) 2008-05-01
EP1527164A2 (en) 2005-05-04
CA2445205A1 (en) 2003-03-27
AU2002353763A2 (en) 2003-04-01
KR100708794B1 (ko) 2007-04-18
US20030087368A1 (en) 2003-05-08
JP2009136285A (ja) 2009-06-25
AU2002353763B2 (en) 2007-01-04
JP2005508629A (ja) 2005-04-07
MXPA03009877A (es) 2005-07-15
WO2003025117A2 (en) 2003-03-27
US20080009609A1 (en) 2008-01-10
AU2009200388A1 (en) 2009-02-19
NZ529910A (en) 2007-03-30
CN1678735A (zh) 2005-10-05
KR20040020896A (ko) 2004-03-09
BR0209425A (pt) 2005-07-19
US7326551B2 (en) 2008-02-05

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