AR033878A1 - Clonacion y secuenciamiento de genes de piruvato descarboxilasa (pdc) de bacterias y usos de los mismos - Google Patents

Clonacion y secuenciamiento de genes de piruvato descarboxilasa (pdc) de bacterias y usos de los mismos

Info

Publication number
AR033878A1
AR033878A1 ARP020101630A ARP020101630A AR033878A1 AR 033878 A1 AR033878 A1 AR 033878A1 AR P020101630 A ARP020101630 A AR P020101630A AR P020101630 A ARP020101630 A AR P020101630A AR 033878 A1 AR033878 A1 AR 033878A1
Authority
AR
Argentina
Prior art keywords
host cells
descarboxylase
piruvato
clonation
pdc
Prior art date
Application number
ARP020101630A
Other languages
English (en)
Original Assignee
Univ Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida filed Critical Univ Florida
Publication of AR033878A1 publication Critical patent/AR033878A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La invención provee moléculas de ácidos nucleicos aislados que codifican enzimas piruvato descarboxilasa que tienen actividad de descarboxilasa, afinidad con el sustrato, termoestabilidad y actividad a diferentes pH mejoradas. Los ácidos nucleicos de la invención también tienen un uso de codon que permite la alta expresión en una variedad de células huéspedes. Por consiguiente, la invención provee vectores de expresión recombinantes que contienen tales moléculas de ácidos nucleicos, células huéspedes recombinantes que comprenden los vectores de expresión, células huéspedes que comprenden otras enzimas etanológenas, y métodos para producir sustancias útiles, por ejemplo, acetaldehído y etanol utilizando tales células huéspedes.
ARP020101630A 2001-05-04 2002-05-03 Clonacion y secuenciamiento de genes de piruvato descarboxilasa (pdc) de bacterias y usos de los mismos AR033878A1 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US28863801P 2001-05-04 2001-05-04
US28867101P 2001-05-04 2001-05-04
US28869901P 2001-05-04 2001-05-04
US28862201P 2001-05-04 2001-05-04
US28869801P 2001-05-04 2001-05-04

Publications (1)

Publication Number Publication Date
AR033878A1 true AR033878A1 (es) 2004-01-07

Family

ID=27540714

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP020101630A AR033878A1 (es) 2001-05-04 2002-05-03 Clonacion y secuenciamiento de genes de piruvato descarboxilasa (pdc) de bacterias y usos de los mismos

Country Status (12)

Country Link
US (2) US7326551B2 (es)
EP (1) EP1527164A4 (es)
JP (3) JP2005508629A (es)
KR (1) KR100708794B1 (es)
CN (1) CN1678735A (es)
AR (1) AR033878A1 (es)
AU (2) AU2002353763B2 (es)
BR (1) BR0209425A (es)
CA (1) CA2445205A1 (es)
MX (1) MXPA03009877A (es)
NZ (1) NZ529910A (es)
WO (1) WO2003025117A2 (es)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19516776A1 (de) * 1995-05-10 1996-11-14 Boehringer Ingelheim Int Chromatin-Regulatorgene
US20020039776A1 (en) * 2000-06-09 2002-04-04 Thomas Jenuwein Mammalian SUV39H2 proteins and isolated DNA molecules encoding them
US6555329B2 (en) * 2000-06-09 2003-04-29 Boehringer Ingelheim International Gmbh Method for identifying compounds altering higher-order chromatin-dependent chromosome stability
ATE458820T1 (de) 2000-06-16 2010-03-15 Res Inst Innovative Tech Earth Verfahren zur herstellung von äthanol unter verwendung eines rekombinanten coryneformen bakteriums
EP1636246B1 (en) * 2003-06-24 2015-08-19 Scientist of Fortune S.A. Production of 2 -deoxynucleosides and 2 -deoxynucleoside precursors from 2-dehydro-3-deoxy-d-gluconate
US7888489B2 (en) * 2005-01-24 2011-02-15 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
CN101395273A (zh) * 2006-01-13 2009-03-25 夏威夷大学 利用蓝藻生产乙醇的方法和组合物
US9284568B2 (en) * 2007-04-03 2016-03-15 Tabletop Energy Methods for transforming eukaryotic algae
US20090246841A1 (en) * 2008-03-26 2009-10-01 Jamieson Andrew C Methods and compositions for production of acetaldehyde
JP2010017094A (ja) * 2008-07-08 2010-01-28 Thermostable Enzyme Laboratory Co Ltd 酵素反応によりアセトアルデヒドを製造する方法
WO2010062707A1 (en) * 2008-10-30 2010-06-03 Joule Unlimited, Inc. Methods and compositions for producing carbon-based products of interest in micro-organisms
AU2009324422A1 (en) * 2008-12-12 2011-07-07 Celexion, Llc Biological synthesis of difunctional alkanes from alpha ketoacids
WO2012125688A2 (en) * 2011-03-14 2012-09-20 Easel Biotechnologies, Llc Microbial syntheses of aldehydes and corresponding alcohols
CA2854450C (en) 2011-11-03 2020-04-21 Easel Biotechnologies, Llc Microbial production of n-butyraldehyde
US20130288320A1 (en) 2012-04-27 2013-10-31 Bioamber Inc. Methods and microorganisms for increasing the biological synthesis of difunctional alkanes
GB201209041D0 (en) 2012-05-22 2012-07-04 Imp Innovations Ltd Protein manipulation
US8906668B2 (en) 2012-11-23 2014-12-09 Seres Health, Inc. Synergistic bacterial compositions and methods of production and use thereof
EP2951285A4 (en) 2013-02-04 2016-10-26 Seres Therapeutics Inc COMPOSITIONS AND METHODS FOR INHIBITING THE GROWTH OF PATHOGENIC BACTERIA
US10973861B2 (en) 2013-02-04 2021-04-13 Seres Therapeutics, Inc. Compositions and methods
EP2967077A4 (en) 2013-03-15 2016-09-14 Seres Therapeutics Inc MICROBIAL COMPOSITIONS AND ASSOCIATED METHODS BASED ON A NETWORK
JP2016165225A (ja) 2013-07-09 2016-09-15 味の素株式会社 有用物質の製造方法
AU2014352643A1 (en) 2013-11-25 2016-06-30 Seres Therapeutics, Inc. Synergistic bacterial compositions and methods of production and use thereof
AU2015264266A1 (en) 2014-05-19 2016-12-01 Memorial Sloan-Kettering Cancer Center Methods and compositions for reducing Clostridium difficile infection
JP5947470B2 (ja) * 2014-05-29 2016-07-06 長瀬産業株式会社 微生物の物質生産性を向上させる方法および該方法に用いるキット
RU2020110462A (ru) 2017-08-14 2021-09-16 Серес Терапеутикс, Инк. Композиции и способы для лечения холестатического заболевания
CN108315365B (zh) * 2018-02-08 2021-01-29 浙江宏元药业股份有限公司 阿托伐他汀中间体的生物合成方法
CN110540952B (zh) * 2019-09-09 2021-05-11 北京科技大学 产叶黄素原核微生物及其在生产叶黄素中的应用
WO2021142019A1 (en) * 2020-01-06 2021-07-15 Solugen, Inc. Compositions, systems and methods for production of value-added chemicals
CN112063638B (zh) * 2020-07-14 2022-03-18 深圳大学 一种从秋茄中克隆扩增出来的丙酮酸脱羧酶基因及其应用
WO2022217117A1 (en) * 2021-04-08 2022-10-13 University Of Florida Research Foundation, Incorporated Enzymatic synthesis of mycosporine-like amino acids

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5839517B2 (ja) 1974-09-20 1983-08-30 カブシキガイシヤ バイオリサ−チセンタ− セルロ−スカラ アルコ−ルオセイゾウスル ホウホウ
US5643758A (en) * 1987-03-10 1997-07-01 New England Biolabs, Inc. Production and purification of a protein fused to a binding protein
DE19523269C2 (de) * 1995-05-26 2000-05-31 Forschungszentrum Juelich Gmbh Verfahren zur Gewinnung von Acyloinen, dafür geeignete Pyruvat-decarboxylase sowie deren Herstellung und DNA-Sequenz des für diese kodierenden PDC-Gens
KR0169913B1 (ko) * 1996-03-14 1999-01-15 김은영 신균주 바실러스속 ds11과 이로부터 생산되는 신규 파이타아제
EP1854889A1 (en) * 1997-02-19 2007-11-14 Enol Energy Inc. Genetically modified cyanobacteria for the production of ethanol
US6225098B1 (en) * 1997-08-25 2001-05-01 Smithkline Beecham Corporation UDP-N-acetylenolpyruvyglucosamine reductase

Also Published As

Publication number Publication date
BR0209425A (pt) 2005-07-19
AU2002353763B2 (en) 2007-01-04
US20030087368A1 (en) 2003-05-08
JP2008099704A (ja) 2008-05-01
WO2003025117A3 (en) 2005-02-10
CN1678735A (zh) 2005-10-05
WO2003025117A2 (en) 2003-03-27
US7326551B2 (en) 2008-02-05
AU2002353763A2 (en) 2003-04-01
AU2009200388A1 (en) 2009-02-19
US20080009609A1 (en) 2008-01-10
EP1527164A2 (en) 2005-05-04
EP1527164A4 (en) 2006-02-01
KR100708794B1 (ko) 2007-04-18
CA2445205A1 (en) 2003-03-27
KR20040020896A (ko) 2004-03-09
JP2009136285A (ja) 2009-06-25
JP2005508629A (ja) 2005-04-07
NZ529910A (en) 2007-03-30
MXPA03009877A (es) 2005-07-15

Similar Documents

Publication Publication Date Title
AR033878A1 (es) Clonacion y secuenciamiento de genes de piruvato descarboxilasa (pdc) de bacterias y usos de los mismos
WO2002031134A3 (en) Novel serine protease genes related to dppiv
AU3246300A (en) Polypeptides having galactose oxidase activity and nucleic acids encoding same
WO2001079444A3 (en) Albumin fusion proteins
DK1131444T3 (da) Polypeptider med lysophospholipase aktivitet og nucleinsyre som koder for disse
CY1112110T1 (el) Κυκλικο μοριο dna το οποιο διαθετει μια συμβατικη αφετηρια αντιγραφης, η διαδικασια παρασκευης του και η χρηση του στην γονιδιακη θεραπεια
EP2298872A3 (en) Polypeptides having lipase activity and polynucleotides encoding same
ATE386127T1 (de) Verbesserung der immunantwort als anwendung in impfstoff und gentherapie
DE69833784D1 (de) Polypeptide mit aminopeptidaseaktivität und für diese kodierende nukleinsäuren
WO2002086144A3 (en) Compositions and methods for recombinational cloning of nucleic acid molecules
ATE450603T1 (de) Polypeptide mit verzweigungsenzym-aktivität und für diese kodierende nukleinsäuren
PE38597A1 (es) Nuevo gen derivado de la bacteria coryneforme y uso del mismo
ID27760A (id) Poliketida-poliketida, pembuatannya, dan bahan-bahan untuk penggunaannya
DE69837475D1 (de) Polypeptide mit aminopeptidaseaktivität und dafür kodierende nukleinsäuren
WO2003040681A3 (de) Gene die für stoffwechselweg-proteine codieren
NZ242124A (en) Dna encoding an enzyme having arabinan-degrading activity
WO2001098469A3 (en) Polypeptides having peroxidase activity and nucleic acids encoding same
HUP0203221A2 (hu) A lignin bioszintézisében résztvevő enzimeket kódoló gének és alkalmazásuk
BR9910787A (pt) Métodos e composições úteis para modulação deangiogênese usando tirosina cinase src
BR9811412A (pt) Variante de enzima subtilase tendo melhorado desempenho de lavagem em detergentes, sequência de dna isolada, vetor de expressão, célula hospedeira microbiana, processos para produzir uma variante e para identificar uma variante de protease, composição, e, uso de uma variante de subtilase
WO2000028043A3 (en) Polypeptides having lactonohydrolase activity and nucleic acids encoding same
ZA200404424B (en) Genes encoding for genetic stability, gene expression and folding proteins.
ATE288491T1 (de) Polypeptide mit 5-aminolevulinsäure synthaseaktivität und deren nukleinsäuren
WO2001092543A3 (en) Improved methods of transfection
ATE317896T1 (de) Polypeptide mit glucanotransferase aktivität und dafür kodierende nukleinsäuren

Legal Events

Date Code Title Description
FB Suspension of granting procedure