AR013017A1 - Composicion que incluye un acido nucleico seleccionado y metodo para su incorporacion en una celula blanco - Google Patents
Composicion que incluye un acido nucleico seleccionado y metodo para su incorporacion en una celula blancoInfo
- Publication number
- AR013017A1 AR013017A1 ARP980106331A ARP980106331A AR013017A1 AR 013017 A1 AR013017 A1 AR 013017A1 AR P980106331 A ARP980106331 A AR P980106331A AR P980106331 A ARP980106331 A AR P980106331A AR 013017 A1 AR013017 A1 AR 013017A1
- Authority
- AR
- Argentina
- Prior art keywords
- nucleic acid
- selected nucleic
- grafted
- hours
- host cell
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Abstract
Un transportador de genes polimérico no-toxico utilizado para introducir un ácido nucleico seleccionado en una célula huesped que comprende un copolímeroinjertado compuesto por polímeros de lado anfifilico de cadena recta, injertados éstosa un polímero principal policationico. El polímeroprincipalpolicationico forma un complejo con ácidos nucleicos por interaccion electrostática. Los polímeros de lado anfifilico reducen la citototocicidad delpolication y aumentan la solubilidad. La proporcion molar de los polímeros de lado puede ajustarse para optimizar la eficiencia de la transfeccion.Igualmente, la relacion del ácido nucleico con el copolímero puede ajustarse para optimizar la eficiencia. La composicion puede utilizarse en un método parala trasnformacion de la célula huésped con un ácido nucleico seleccionado. Los copolímeros injertados de polietilenglicol y poli-L-lisina (PEG-g-PLL) resultanparticularmente efectivos y ejecutan picos de expresiones genéticas dentro de las 24 horas, con persistencia de por lo menos 96 horas.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US6935197P | 1997-12-12 | 1997-12-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR013017A1 true AR013017A1 (es) | 2000-11-22 |
Family
ID=22088387
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP980106331A AR013017A1 (es) | 1997-12-12 | 1998-12-11 | Composicion que incluye un acido nucleico seleccionado y metodo para su incorporacion en una celula blanco |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1032659A1 (es) |
| JP (1) | JP2001526181A (es) |
| KR (1) | KR20010032879A (es) |
| AR (1) | AR013017A1 (es) |
| AU (1) | AU1820999A (es) |
| BR (1) | BR9814273A (es) |
| IL (1) | IL136679A0 (es) |
| WO (1) | WO1999029839A1 (es) |
| ZA (1) | ZA9811378B (es) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001060415A1 (en) * | 2000-02-18 | 2001-08-23 | The Immune Response Corporation | Methods and compositions for gene delivery |
| EP1421217A2 (de) * | 2001-08-27 | 2004-05-26 | Zeptosens AG | Oberfläche zur immobilisierung von nukleinsäuren |
| CN100434520C (zh) * | 2006-03-07 | 2008-11-19 | 湖北大学 | 聚肽型转染试剂 |
| CN102462846B (zh) * | 2010-11-08 | 2014-07-09 | 复旦大学 | 一种氯代毒素修饰的脑胶质瘤靶向基因递释复合物及其制备方法 |
| KR101357899B1 (ko) * | 2011-02-25 | 2014-02-03 | 서울대학교산학협력단 | 간세포 표적 유전자 전달체로서 갈락토실화 폴리에틸렌글리콜-키토산-그라프트-스페르민 공중합체 및 이를 이용한 유전자 치료 |
| WO2012118189A1 (ja) | 2011-03-03 | 2012-09-07 | 中外製薬株式会社 | アミノ－カルボン酸により修飾されたヒアルロン酸誘導体 |
| KR101486989B1 (ko) | 2013-10-15 | 2015-02-03 | 부산대학교 산학협력단 | 공중합체 및 이를 포함하는 유전자 전달체 |
| EP3079668A1 (en) | 2013-12-09 | 2016-10-19 | Durect Corporation | Pharmaceutically active agent complexes, polymer complexes, and compositions and methods involving the same |
| CN103755953B (zh) * | 2013-12-12 | 2015-11-18 | 深圳先进技术研究院 | 一种智能聚阳离子纳米载体、其制备方法及其应用 |
| CN106554499B (zh) * | 2015-09-25 | 2018-12-14 | 南京理工大学 | 一种含二硫键的聚(β-氨基酯)类聚合物基因载体及其合成方法和应用 |
| CN106978444B (zh) * | 2016-01-15 | 2021-12-17 | 江苏命码生物科技有限公司 | 一种向细胞中导入核酸的方法 |
| CN111393552B (zh) * | 2020-03-22 | 2022-07-15 | 福建医科大学 | 聚乙烯胺-精氨酸接枝衍生物作为基因载体的制备和应用 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994018834A1 (en) * | 1993-02-16 | 1994-09-01 | Virginia Tech Intellectual Properties, Inc. | Polyelectrolyte dna conjugation and genetic transformation of an animal |
-
1998
- 1998-12-11 AR ARP980106331A patent/AR013017A1/es not_active Application Discontinuation
- 1998-12-11 ZA ZA9811378A patent/ZA9811378B/xx unknown
- 1998-12-11 AU AU18209/99A patent/AU1820999A/en not_active Abandoned
- 1998-12-11 WO PCT/US1998/026451 patent/WO1999029839A1/en not_active Application Discontinuation
- 1998-12-11 KR KR1020007006209A patent/KR20010032879A/ko active Search and Examination
- 1998-12-11 IL IL13667998A patent/IL136679A0/xx unknown
- 1998-12-11 EP EP98963119A patent/EP1032659A1/en not_active Withdrawn
- 1998-12-11 BR BR9814273-9A patent/BR9814273A/pt not_active Application Discontinuation
- 1998-12-11 JP JP2000524412A patent/JP2001526181A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR20010032879A (ko) | 2001-04-25 |
| IL136679A0 (en) | 2001-06-14 |
| AU1820999A (en) | 1999-06-28 |
| EP1032659A1 (en) | 2000-09-06 |
| ZA9811378B (en) | 1999-08-05 |
| BR9814273A (pt) | 2000-10-03 |
| JP2001526181A (ja) | 2001-12-18 |
| WO1999029839A1 (en) | 1999-06-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal |