AR013017A1 - COMPOSITION INCLUDING A SELECTED NUCLEIC ACID AND METHOD FOR ITS INCORPORATION IN A WHITE CELL - Google Patents

COMPOSITION INCLUDING A SELECTED NUCLEIC ACID AND METHOD FOR ITS INCORPORATION IN A WHITE CELL

Info

Publication number
AR013017A1
AR013017A1 ARP980106331A ARP980106331A AR013017A1 AR 013017 A1 AR013017 A1 AR 013017A1 AR P980106331 A ARP980106331 A AR P980106331A AR P980106331 A ARP980106331 A AR P980106331A AR 013017 A1 AR013017 A1 AR 013017A1
Authority
AR
Argentina
Prior art keywords
nucleic acid
selected nucleic
grafted
hours
host cell
Prior art date
Application number
ARP980106331A
Other languages
Spanish (es)
Original Assignee
Expression Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Expression Genetics Inc filed Critical Expression Genetics Inc
Publication of AR013017A1 publication Critical patent/AR013017A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Other Resins Obtained By Reactions Not Involving Carbon-To-Carbon Unsaturated Bonds (AREA)
  • Medicinal Preparation (AREA)

Abstract

Un transportador de genes polimérico no-toxico utilizado para introducir un ácido nucleico seleccionado en una célula huesped que comprende un copolímeroinjertado compuesto por polímeros de lado anfifilico de cadena recta, injertados éstosa un polímero principal policationico. El polímeroprincipalpolicationico forma un complejo con ácidos nucleicos por interaccion electrostática. Los polímeros de lado anfifilico reducen la citototocicidad delpolication y aumentan la solubilidad. La proporcion molar de los polímeros de lado puede ajustarse para optimizar la eficiencia de la transfeccion.Igualmente, la relacion del ácido nucleico con el copolímero puede ajustarse para optimizar la eficiencia. La composicion puede utilizarse en un método parala trasnformacion de la célula huésped con un ácido nucleico seleccionado. Los copolímeros injertados de polietilenglicol y poli-L-lisina (PEG-g-PLL) resultanparticularmente efectivos y ejecutan picos de expresiones genéticas dentro de las 24 horas, con persistencia de por lo menos 96 horas.A non-toxic polymeric gene transporter used to introduce a selected nucleic acid into a host cell comprising a grafted copolymer composed of straight-chain amphiphilic side polymers, grafted to a polycationic main polymer. The primary polycationic polymer forms a complex with nucleic acids by electrostatic interaction. Amphiphilic side polymers reduce the cytotoxicity of polycation and increase solubility. The molar ratio of the side polymers can be adjusted to optimize transfection efficiency. Similarly, the ratio of the nucleic acid to the copolymer can be adjusted to optimize efficiency. The composition can be used in a method for transforming the host cell with a selected nucleic acid. Grafted copolymers of polyethylene glycol and poly-L-lysine (PEG-g-PLL) are particularly effective and perform gene expression peaks within 24 hours, with persistence of at least 96 hours.

ARP980106331A 1997-12-12 1998-12-11 COMPOSITION INCLUDING A SELECTED NUCLEIC ACID AND METHOD FOR ITS INCORPORATION IN A WHITE CELL AR013017A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US6935197P 1997-12-12 1997-12-12

Publications (1)

Publication Number Publication Date
AR013017A1 true AR013017A1 (en) 2000-11-22

Family

ID=22088387

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP980106331A AR013017A1 (en) 1997-12-12 1998-12-11 COMPOSITION INCLUDING A SELECTED NUCLEIC ACID AND METHOD FOR ITS INCORPORATION IN A WHITE CELL

Country Status (9)

Country Link
EP (1) EP1032659A1 (en)
JP (1) JP2001526181A (en)
KR (1) KR20010032879A (en)
AR (1) AR013017A1 (en)
AU (1) AU1820999A (en)
BR (1) BR9814273A (en)
IL (1) IL136679A0 (en)
WO (1) WO1999029839A1 (en)
ZA (1) ZA9811378B (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001060415A1 (en) * 2000-02-18 2001-08-23 The Immune Response Corporation Methods and compositions for gene delivery
EP1421217A2 (en) * 2001-08-27 2004-05-26 Zeptosens AG Surface for the immobilisation of nucleic acids
CN100434520C (en) * 2006-03-07 2008-11-19 湖北大学 Polypeptide type transfection reagent
CN102462846B (en) * 2010-11-08 2014-07-09 复旦大学 Chlorotoxin-modified glioma targeting gene delivery compound and preparation method thereof
KR101357899B1 (en) * 2011-02-25 2014-02-03 서울대학교산학협력단 Galactosylated polyethylene glycol-chitosan-graft-spermine copolymer as a hepatocyte targeting gene carrier and gene therapy using the same
SI2682409T1 (en) 2011-03-03 2017-08-31 Chugai Seiyaku Kabushiki Kaisha Derivative of hyaluronic acid modified with amino-carboxylic acid
KR101486989B1 (en) 2013-10-15 2015-02-03 부산대학교 산학협력단 Copolymer and gene transfer carrier comprising the same
JP6564369B2 (en) 2013-12-09 2019-08-21 デュレクト コーポレイション Pharmaceutically active agent conjugates, polymer conjugates, and compositions and methods involving them
CN103755953B (en) * 2013-12-12 2015-11-18 深圳先进技术研究院 A kind of intelligent polycation nano-carrier, its preparation method and application thereof
CN106554499B (en) * 2015-09-25 2018-12-14 南京理工大学 A kind of poly- (beta-amino ester) quasi polymer genophore and its synthetic method and application containing disulfide bond
CN106978444B (en) * 2016-01-15 2021-12-17 江苏命码生物科技有限公司 Method for introducing nucleic acid into cell
CN111393552B (en) * 2020-03-22 2022-07-15 福建医科大学 Preparation and application of polyvinyl amine-arginine grafted derivative as gene vector

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994018834A1 (en) * 1993-02-16 1994-09-01 Virginia Tech Intellectual Properties, Inc. Polyelectrolyte dna conjugation and genetic transformation of an animal

Also Published As

Publication number Publication date
WO1999029839A1 (en) 1999-06-17
JP2001526181A (en) 2001-12-18
IL136679A0 (en) 2001-06-14
AU1820999A (en) 1999-06-28
BR9814273A (en) 2000-10-03
ZA9811378B (en) 1999-08-05
EP1032659A1 (en) 2000-09-06
KR20010032879A (en) 2001-04-25

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