ZA201101367B - Identification and use of bacterial [2fe-2s] dihydroxy-acid dehydratases - Google Patents

Identification and use of bacterial [2fe-2s] dihydroxy-acid dehydratases

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Publication number
ZA201101367B
ZA201101367B ZA2011/01367A ZA201101367A ZA201101367B ZA 201101367 B ZA201101367 B ZA 201101367B ZA 2011/01367 A ZA2011/01367 A ZA 2011/01367A ZA 201101367 A ZA201101367 A ZA 201101367A ZA 201101367 B ZA201101367 B ZA 201101367B
Authority
ZA
South Africa
Prior art keywords
dihydroxy
bacterial
identification
acid dehydratases
dehydratases
Prior art date
Application number
ZA2011/01367A
Other languages
English (en)
Inventor
Dennis Flint
Steven Cary Rothman
Wonchul Suh
Jean-Francois Tomb
Rick W Ye
Original Assignee
Butamax (Tm) Advanced Biofuels Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Butamax (Tm) Advanced Biofuels Llc filed Critical Butamax (Tm) Advanced Biofuels Llc
Publication of ZA201101367B publication Critical patent/ZA201101367B/en

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    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/06Alanine; Leucine; Isoleucine; Serine; Homoserine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/08Lysine; Diaminopimelic acid; Threonine; Valine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16Butanols
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/01Hydro-lyases (4.2.1)
    • C12Y402/01009Dihydroxy-acid dehydratase (4.2.1.9), i.e. acetohydroxyacid dehydratase
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10Sequence alignment; Homology search
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Theoretical Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ZA2011/01367A 2008-09-29 2011-02-21 Identification and use of bacterial [2fe-2s] dihydroxy-acid dehydratases ZA201101367B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10079208P 2008-09-29 2008-09-29
PCT/US2009/058827 WO2010037112A1 (en) 2008-09-29 2009-09-29 IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES

Publications (1)

Publication Number Publication Date
ZA201101367B true ZA201101367B (en) 2014-03-26

Family

ID=41402186

Family Applications (2)

Application Number Title Priority Date Filing Date
ZA2011/01367A ZA201101367B (en) 2008-09-29 2011-02-21 Identification and use of bacterial [2fe-2s] dihydroxy-acid dehydratases
ZA2013/09658A ZA201309658B (en) 2008-09-29 2013-12-20 Identification and use of bacterial [2fe-2s]dihydroxy-acid dehydratases

Family Applications After (1)

Application Number Title Priority Date Filing Date
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Country Status (13)

Country Link
US (3) US20100081154A1 (enExample)
EP (2) EP2337851B1 (enExample)
JP (1) JP5846912B2 (enExample)
KR (1) KR101659101B1 (enExample)
CN (1) CN102186973B (enExample)
AU (1) AU2009296225B2 (enExample)
BR (1) BRPI0913682A2 (enExample)
CA (1) CA2735690A1 (enExample)
ES (1) ES2521675T3 (enExample)
MX (1) MX2011003238A (enExample)
NZ (1) NZ591244A (enExample)
WO (1) WO2010037112A1 (enExample)
ZA (2) ZA201101367B (enExample)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9303225B2 (en) 2005-10-26 2016-04-05 Butamax Advanced Biofuels Llc Method for the production of isobutanol by recombinant yeast
US8956850B2 (en) 2008-06-05 2015-02-17 Butamax Advanced Biofuels Llc Enhanced pyruvate to acetolactate conversion in yeast
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
WO2008130995A2 (en) 2007-04-18 2008-10-30 E. I. Du Pont De Nemours And Company Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
US20160222370A1 (en) * 2008-09-29 2016-08-04 Butamax Advanced Biofuels Llc Recombinant Yeast Host Cell With Fe-S Cluster Proteins And Methods Of Using Thereof
AU2009296227A1 (en) * 2008-09-29 2010-04-01 Butamax(Tm) Advanced Biofuels Llc Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
BRPI0913681A2 (pt) * 2008-09-29 2019-09-24 Butamax Advanced Biofuels Llc "célula bacteriana ácido-lática recombinante e método de produção de isobutanol"
US20100081154A1 (en) 2008-09-29 2010-04-01 Butamax(Tm) Advanced Biofuels Llc IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES
AU2009296224B2 (en) 2008-09-29 2015-02-12 Butamax(Tm) Advanced Biofuels Llc Increased heterologous Fe-S enzyme actiivty in yeast
US8465964B2 (en) 2008-11-13 2013-06-18 Butamax (TM) Advanced Biofules LLC Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis
US8614085B2 (en) * 2009-02-27 2013-12-24 Butamax(Tm) Advanced Biofuels Llc Yeast with increased butanol tolerance involving a multidrug efflux pump gene
EP2419517A2 (en) 2009-04-13 2012-02-22 Butamax(TM) Advanced Biofuels LLC Method for producing butanol using extractive fermentation
US8232089B2 (en) * 2009-08-12 2012-07-31 Gevo, Inc. Cytosolic isobutanol pathway localization for the production of isobutanol
BR112012006939A2 (pt) * 2009-09-29 2015-09-08 Butamax Tm Advanced Biofuels célula hospedeira de produção de levedura recombinante e método para a produção de um produto selecionado do grupo que consiste em 2,3-butanodiol, isobutanol, 2-butanol, 1-butanol, 2-butanona, valina, leucina, ácido lático, ácido málico, álcool, isoampilico e isoprenoides
JP5805094B2 (ja) 2009-09-29 2015-11-04 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー 高有効性のケトール酸レダクトイソメラーゼ酵素を用いたイソブタノールの発酵生産
US20110195505A1 (en) * 2009-10-08 2011-08-11 Butamax(Tm) Advanced Biofuels Llc Bacterial strains for butanol production
JP2013511283A (ja) * 2009-11-23 2013-04-04 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー 電解質の添加を伴う抽出発酵を用いたブタノールの生成方法
JP2013511284A (ja) * 2009-11-23 2013-04-04 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー オスモライトの添加を伴う抽出発酵を用いたブタノールの生成方法
GB2487889B (en) 2009-11-24 2012-12-19 Gevo Inc Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids
EP2519628A2 (en) 2009-12-29 2012-11-07 Butamax (TM) Advanced Biofuels LLC Alcohol dehydrogenases (adh) useful for fermentive production of lower alkyl alcohols
AU2010339511A1 (en) 2009-12-29 2012-07-05 Butamax(Tm) Advanced Biofuels Llc Expression of hexose kinase in recombinant host cells
KR20130027063A (ko) 2010-02-17 2013-03-14 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 Fe-s 클러스터 요구성 단백질의 활성 향상
WO2011149774A1 (en) * 2010-05-24 2011-12-01 Xyleco, Inc. Processing biomass
MX2012014550A (es) 2010-06-17 2013-01-29 Butamax Tm Advanced Biofuels Cultivo de produccion de levadura para la produccion de butanol.
US8871488B2 (en) 2010-06-18 2014-10-28 Butamax Advanced Biofuels Llc Recombinant host cells comprising phosphoketolases
US9012190B2 (en) 2011-06-15 2015-04-21 Butamax Advanced Biofuels Llc Use of thiamine and nicotine adenine dinucleotide for butanol production
US9040263B2 (en) 2010-07-28 2015-05-26 Butamax Advanced Biofuels Llc Production of alcohol esters and in situ product removal during alcohol fermentation
MX2012014551A (es) 2010-06-18 2013-02-07 Butamax Tm Advanced Biofuels Suplementacion de acidos grasos para mejorar la productividad de alcohol.
JP2013528399A (ja) 2010-06-18 2013-07-11 ブタマックス(ティーエム) アドバンスド バイオフューエルズ エルエルシー 抽出発酵におけるアルコール除去のための、油に由来する抽出溶媒
CA2810244C (en) 2010-09-07 2020-10-06 Butamax(Tm) Advanced Biofuels Llc Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US9790521B2 (en) 2011-03-24 2017-10-17 Butamax Advanced Biofuels Llc Host cells and methods for production of isobutanol
BR112013032319A2 (pt) 2011-06-17 2016-12-20 Butamax Advanced Biofuels Llc métodos da geração de coprodutos de destilação, coprodutos de destilação, método para atenuar o impacto de contaminantes de fermentação, método para a redução da contaminação por micotoxinas, método para a redução da variabilidade do teor de lipídeos, método para aumento do teor de triglicerídeos, método de produção de um coproduto de destilação para o combustível e método de produção de um coproduto destilação para o biodiesel
CN105473726B (zh) 2011-07-28 2021-03-12 布特马斯先进生物燃料有限责任公司 酮异戊酸脱羧酶及其使用方法
WO2013043801A1 (en) * 2011-09-20 2013-03-28 Gevo, Inc. High-performance dihydroxy acid dehydratases
JP2015501643A (ja) 2011-12-09 2015-01-19 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー 発酵ブロスから生成物アルコールを取り出す方法
JP2015503350A (ja) 2011-12-30 2015-02-02 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー アルコールの発酵生産
WO2013102147A2 (en) 2011-12-30 2013-07-04 Butamax (Tm) Advanced Biofuels Llc Genetic switches for butanol production
AU2013235391A1 (en) 2012-03-23 2014-09-04 Butamax Advanced Biofuels Llc Acetate supplemention of medium for butanologens
CA2873109A1 (en) 2012-05-11 2013-11-28 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase enzymes and methods of use
US20140024064A1 (en) 2012-07-23 2014-01-23 Butamax(Tm) Advanced Biofuels Llc Processes and systems for the production of fermentative alcohols
CA2881407A1 (en) 2012-08-22 2014-02-27 Butamax Advanced Biofuels Llc Production of fermentation products
WO2014043288A1 (en) 2012-09-12 2014-03-20 Butamax Advanced Biofuels Llc Processes and systems for the production of fermentation products
US9840724B2 (en) 2012-09-21 2017-12-12 Butamax Advanced Biofuels Llc Production of renewable hydrocarbon compositions
BR112015006691A2 (pt) 2012-09-26 2017-12-12 Butamax Advanced Biofuels Llc métodos, célula hospedeira recombinante, composição, polipeptídeo, polinucleotídeos e vetor recombinante
AU2013323396B2 (en) 2012-09-28 2017-04-20 Butamax Advanced Biofuels Llc Production of fermentation products
US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
AU2013329024A1 (en) 2012-10-11 2015-03-12 Butamax Advanced Biofuels Llc Processes and systems for the production of fermentation products
WO2014106107A2 (en) 2012-12-28 2014-07-03 Butamax (Tm) Advanced Biofuels Llc Dhad variants for butanol production
WO2014105840A1 (en) 2012-12-31 2014-07-03 Butamax Advanced Biofuels Llc Fermentative production of alcohols
WO2014159309A1 (en) 2013-03-12 2014-10-02 Butamax Advanced Biofuels Llc Processes and systems for the production of alcohols
CN105378057A (zh) 2013-03-14 2016-03-02 纳幕尔杜邦公司 用于丁醇生产的甘油3-磷酸脱氢酶
WO2014151645A1 (en) 2013-03-15 2014-09-25 Butamax Advanced Biofuels Llc Process for maximizing biomass growth and butanol yield by feedback control
US9771602B2 (en) 2013-03-15 2017-09-26 Butamax Advanced Biofuels Llc Competitive growth and/or production advantage for butanologen microorganism
WO2014151190A1 (en) 2013-03-15 2014-09-25 Butamax Advanced Biofuels Llc Dhad variants and methods of screening
WO2014144728A1 (en) 2013-03-15 2014-09-18 Butamax Advanced Biofuels Llc Method for production of butanol using extractive fermentation
WO2014144643A1 (en) 2013-03-15 2014-09-18 Butamax Advanced Biofuels Llc Method for producing butanol using extractive fermentation
US9663759B2 (en) 2013-07-03 2017-05-30 Butamax Advanced Biofuels Llc Partial adaptation for butanol production
US10280438B2 (en) 2014-08-11 2019-05-07 Butamax Advanced Biofuels Llc Method for the production of yeast
WO2018157942A1 (en) 2017-03-03 2018-09-07 Nmr - Bio Cell-free synthesis of isotopic labelled proteins from amino-acids precursors
CN111893083A (zh) * 2019-05-05 2020-11-06 中国科学院上海高等研究院 改造的克雷伯氏属细菌及其生产2,3-二羟基异戊酸的应用
DE102022119024A1 (de) * 2022-07-28 2024-02-08 INM - Leibniz-Institut für Neue Materialien gemeinnützige Gesellschaft mit beschränkter Haftung Neuartige genetische Werkzeuge

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
FR2696190B1 (fr) * 1992-09-25 1994-12-09 Agronomique Inst Nat Rech Acide nucléique codant pour une alpha-acétolactate synthase et ses applications.
US6800744B1 (en) * 1997-07-02 2004-10-05 Genome Therapeutics Corporation Nucleic acid and amino acid sequences relating to Streptococcus pneumoniae for diagnostics and therapeutics
DE19855312A1 (de) 1998-12-01 2000-06-08 Degussa Verfahren zur fermentativen Herstellung von D-Pantothensäure unter Verwendung coryneformer Bakterien
EP1887081A2 (en) * 1999-02-25 2008-02-13 Ceres Incorporated DNA Sequences
US7273769B1 (en) 2000-08-16 2007-09-25 Micron Technology, Inc. Method and apparatus for removing encapsulating material from a packaged microelectronic device
CA2430270A1 (en) * 2000-11-22 2002-05-30 Cargill Dow Polymers, Llc Methods and materials for the synthesis of organic products
US20110081348A1 (en) 2004-12-01 2011-04-07 Nina Brogden Fungal signalling and metabolic enzymes
JP4991700B2 (ja) * 2005-04-12 2012-08-01 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー バイオマス処理のためのシステムおよび方法
EP1913022A1 (en) * 2005-08-12 2008-04-23 Suntory Limited Dihydroxy-acid dehydratase gene and use thereof
EP3301182B1 (en) * 2005-10-26 2020-03-18 Butamax (TM) Advanced Biofuels LLC Fermentative production of four carbon alcohols
US8956850B2 (en) * 2008-06-05 2015-02-17 Butamax Advanced Biofuels Llc Enhanced pyruvate to acetolactate conversion in yeast
BRPI0709607B1 (pt) 2006-03-13 2018-05-08 Cargill Inc cédulas de levedura tendo via rompida de diidroxi acetona fosfato para glicerol e processo de fermentação"
US8962298B2 (en) * 2006-05-02 2015-02-24 Butamax Advanced Biofuels Llc Recombinant host cell comprising a diol dehydratase
WO2008098227A2 (en) 2007-02-09 2008-08-14 The Regents Of The University Of California Biofuel production by recombinant microorganisms
WO2008130995A2 (en) 2007-04-18 2008-10-30 E. I. Du Pont De Nemours And Company Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
CA2710359C (en) 2007-12-23 2018-02-20 Gevo, Inc. Yeast organism producing isobutanol at a high yield
DE102008010121B4 (de) 2008-02-20 2013-11-21 Butalco Gmbh Fermentative Produktion von Isobutanol mit Hefe
MX2010013182A (es) * 2008-06-04 2011-01-14 Butamax Tm Advanced Biofuels Metodo para producir butanol mediante el uso de fermentacion extractiva de dos fases.
AU2009296227A1 (en) 2008-09-29 2010-04-01 Butamax(Tm) Advanced Biofuels Llc Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
AU2009296224B2 (en) 2008-09-29 2015-02-12 Butamax(Tm) Advanced Biofuels Llc Increased heterologous Fe-S enzyme actiivty in yeast
BRPI0913681A2 (pt) 2008-09-29 2019-09-24 Butamax Advanced Biofuels Llc "célula bacteriana ácido-lática recombinante e método de produção de isobutanol"
US20100081154A1 (en) 2008-09-29 2010-04-01 Butamax(Tm) Advanced Biofuels Llc IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES
AU2009296232A1 (en) 2008-09-29 2010-04-01 Butamax(Tm) Advanced Biofuels Llc Enhanced iron-sulfur cluster formation for increased dihydroxy-acid dehydratase activity in lactic acid bacteria
US8828694B2 (en) * 2008-11-13 2014-09-09 Butamax Advanced Biofuels Llc Production of isobutanol in yeast mitochondria
US8232089B2 (en) * 2009-08-12 2012-07-31 Gevo, Inc. Cytosolic isobutanol pathway localization for the production of isobutanol
JP5805094B2 (ja) * 2009-09-29 2015-11-04 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー 高有効性のケトール酸レダクトイソメラーゼ酵素を用いたイソブタノールの発酵生産
BR112012006939A2 (pt) * 2009-09-29 2015-09-08 Butamax Tm Advanced Biofuels célula hospedeira de produção de levedura recombinante e método para a produção de um produto selecionado do grupo que consiste em 2,3-butanodiol, isobutanol, 2-butanol, 1-butanol, 2-butanona, valina, leucina, ácido lático, ácido málico, álcool, isoampilico e isoprenoides
JP2013511284A (ja) * 2009-11-23 2013-04-04 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー オスモライトの添加を伴う抽出発酵を用いたブタノールの生成方法
JP2013511283A (ja) * 2009-11-23 2013-04-04 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー 電解質の添加を伴う抽出発酵を用いたブタノールの生成方法

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NZ591244A (en) 2013-03-28
CA2735690A1 (en) 2010-04-01
JP5846912B2 (ja) 2016-01-20
US8951937B2 (en) 2015-02-10
KR20110063575A (ko) 2011-06-10
US20140051137A1 (en) 2014-02-20
AU2009296225A1 (en) 2010-04-01
MX2011003238A (es) 2011-04-28
US20100081154A1 (en) 2010-04-01
AU2009296225B2 (en) 2015-09-17
EP2337851A1 (en) 2011-06-29
EP2821484A1 (en) 2015-01-07
ZA201309658B (en) 2016-06-29
ES2521675T3 (es) 2014-11-13
CN102186973A (zh) 2011-09-14
US20140030776A1 (en) 2014-01-30
CN102186973B (zh) 2017-08-15
JP2012503993A (ja) 2012-02-16
EP2821484B1 (en) 2016-06-29
WO2010037112A1 (en) 2010-04-01
EP2337851B1 (en) 2014-08-13
KR101659101B1 (ko) 2016-09-22

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