AU2009296225B2 - Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases - Google Patents

Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases Download PDF

Info

Publication number
AU2009296225B2
AU2009296225B2 AU2009296225A AU2009296225A AU2009296225B2 AU 2009296225 B2 AU2009296225 B2 AU 2009296225B2 AU 2009296225 A AU2009296225 A AU 2009296225A AU 2009296225 A AU2009296225 A AU 2009296225A AU 2009296225 B2 AU2009296225 B2 AU 2009296225B2
Authority
AU
Australia
Prior art keywords
dhad
sequences
bacterial
sequence
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2009296225A
Other languages
English (en)
Other versions
AU2009296225A1 (en
Inventor
Dennis Flint
Steven Cary Rothman
Wonchul Suh
Jean-Francois Tomb
Rick W. Ye
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Butamax Advanced Biofuels LLC
Original Assignee
Butamax Advanced Biofuels LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Butamax Advanced Biofuels LLC filed Critical Butamax Advanced Biofuels LLC
Publication of AU2009296225A1 publication Critical patent/AU2009296225A1/en
Assigned to BUTAMAX(TM) ADVANCED BIOFUELS LLC reassignment BUTAMAX(TM) ADVANCED BIOFUELS LLC Amend patent request/document other than specification (104) Assignors: BUTAMAX ADVANCED BIOFUELS LLC
Application granted granted Critical
Publication of AU2009296225B2 publication Critical patent/AU2009296225B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01009—Dihydroxy-acid dehydratase (4.2.1.9), i.e. acetohydroxyacid dehydratase
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Theoretical Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2009296225A 2008-09-29 2009-09-29 Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases Active AU2009296225B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10079208P 2008-09-29 2008-09-29
US61/100,792 2008-09-29
PCT/US2009/058827 WO2010037112A1 (en) 2008-09-29 2009-09-29 IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES

Publications (2)

Publication Number Publication Date
AU2009296225A1 AU2009296225A1 (en) 2010-04-01
AU2009296225B2 true AU2009296225B2 (en) 2015-09-17

Family

ID=41402186

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2009296225A Active AU2009296225B2 (en) 2008-09-29 2009-09-29 Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases

Country Status (13)

Country Link
US (3) US20100081154A1 (enExample)
EP (2) EP2337851B1 (enExample)
JP (1) JP5846912B2 (enExample)
KR (1) KR101659101B1 (enExample)
CN (1) CN102186973B (enExample)
AU (1) AU2009296225B2 (enExample)
BR (1) BRPI0913682A2 (enExample)
CA (1) CA2735690A1 (enExample)
ES (1) ES2521675T3 (enExample)
MX (1) MX2011003238A (enExample)
NZ (1) NZ591244A (enExample)
WO (1) WO2010037112A1 (enExample)
ZA (2) ZA201101367B (enExample)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9303225B2 (en) 2005-10-26 2016-04-05 Butamax Advanced Biofuels Llc Method for the production of isobutanol by recombinant yeast
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
BRPI0808600A2 (pt) 2007-04-18 2018-12-26 Du Pont "método para a conversão de acetolactato em dihidroxi-isovalerato, método para a produção de isobutanol, célula hospedeira recombinante e método para a identificação e isolameno de uma construção genética"
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
BRPI0909989A2 (pt) * 2008-06-05 2021-06-22 Butamax Advanced Biofuels Llc célula de levedura recombinante e método para a produção de um produto
US20160222370A1 (en) * 2008-09-29 2016-08-04 Butamax Advanced Biofuels Llc Recombinant Yeast Host Cell With Fe-S Cluster Proteins And Methods Of Using Thereof
WO2010037111A1 (en) 2008-09-29 2010-04-01 Butamax™ Advanced Biofuels LLC INCREASED HETEROLOGOUS Fe-S ENZYME ACTIIVTY IN YEAST
EP2337851B1 (en) 2008-09-29 2014-08-13 Butamax Advanced Biofuels Llc IDENTIFICATION AND USE OF BACTERIAL Ý2Fe-2S¨DIHYDROXY-ACID DEHYDRATASES
WO2010037105A1 (en) * 2008-09-29 2010-04-01 Butamax™ Advanced Biofuels LLC Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria
EP2337869B1 (en) 2008-09-29 2015-08-19 Butamax Advanced Biofuels Llc Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
US8465964B2 (en) * 2008-11-13 2013-06-18 Butamax (TM) Advanced Biofules LLC Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis
US8614085B2 (en) * 2009-02-27 2013-12-24 Butamax(Tm) Advanced Biofuels Llc Yeast with increased butanol tolerance involving a multidrug efflux pump gene
EP2419517A2 (en) 2009-04-13 2012-02-22 Butamax(TM) Advanced Biofuels LLC Method for producing butanol using extractive fermentation
BR112012003241A2 (pt) * 2009-08-12 2015-09-01 Gevo Inc Localização de via de isobutanol citosólico para a produção de isobutanol
IN2012DN02227A (enExample) 2009-09-29 2015-08-21 Butamax Tm Advanced Biofuels
US9260708B2 (en) * 2009-09-29 2016-02-16 Butamax Advanced Biofuels Llc Yeast production host cells
US20110195505A1 (en) * 2009-10-08 2011-08-11 Butamax(Tm) Advanced Biofuels Llc Bacterial strains for butanol production
EP2504447A2 (en) 2009-11-23 2012-10-03 ButamaxTM Advanced Biofuels LLC Method for producing butanol using extractive fermentation with osmolyte addition
CN102656272A (zh) * 2009-11-23 2012-09-05 布特马斯先进生物燃料有限责任公司 使用添加电解质的萃取发酵来生产丁醇的方法
WO2011066356A1 (en) * 2009-11-24 2011-06-03 Gevo, Inc. Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids
CA2785660A1 (en) 2009-12-29 2011-07-07 Butamax(Tm) Advanced Biofuels Llc Expression of hexose kinase in recombinant host cells
BR112012016042A2 (pt) 2009-12-29 2020-09-15 Butamax Advanced Biofuels Llc "celula hospedeira microbiana recombinante, metodo para a produção de isobutanol, método para a produção de 2-butanol e método para a produção de 14-butanol
MX357290B (es) 2010-02-17 2018-07-04 Butamax Tm Advanced Biofuels Mejoramiento de la actividad de proteínas que requieren centros fe-s.
EP2576213A4 (en) * 2010-05-24 2016-07-13 Xyleco Inc BIOMASS TREATMENT
KR20130027551A (ko) 2010-06-17 2013-03-15 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 부탄올 생산을 위한 효모 생산 배양물
CA2800542A1 (en) 2010-06-18 2011-12-22 Butamax(Tm) Advanced Biofuels Llc Supplementation of fatty acids for improving alcohol productivity
US8871488B2 (en) 2010-06-18 2014-10-28 Butamax Advanced Biofuels Llc Recombinant host cells comprising phosphoketolases
CN103119172B (zh) 2010-06-18 2016-05-11 布特马斯先进生物燃料有限责任公司 在提取发酵中用于醇移除的来源于油的提取溶剂
US9040263B2 (en) 2010-07-28 2015-05-26 Butamax Advanced Biofuels Llc Production of alcohol esters and in situ product removal during alcohol fermentation
US9012190B2 (en) 2011-06-15 2015-04-21 Butamax Advanced Biofuels Llc Use of thiamine and nicotine adenine dinucleotide for butanol production
CN105950525A (zh) 2010-09-07 2016-09-21 布特马斯先进生物燃料有限责任公司 整合编码催化丙酮酸至乙酰乳酸的转化的多肽的多核苷酸
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
KR20140092759A (ko) 2011-03-24 2014-07-24 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 숙주 세포 및 아이소부탄올의 제조 방법
BR112013032319A2 (pt) 2011-06-17 2016-12-20 Butamax Advanced Biofuels Llc métodos da geração de coprodutos de destilação, coprodutos de destilação, método para atenuar o impacto de contaminantes de fermentação, método para a redução da contaminação por micotoxinas, método para a redução da variabilidade do teor de lipídeos, método para aumento do teor de triglicerídeos, método de produção de um coproduto de destilação para o combustível e método de produção de um coproduto destilação para o biodiesel
JP6093764B2 (ja) 2011-07-28 2017-03-08 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー ケトイソ吉草酸デカルボキシラーゼ酵素およびその使用方法
WO2013043801A1 (en) * 2011-09-20 2013-03-28 Gevo, Inc. High-performance dihydroxy acid dehydratases
EP2788096A2 (en) 2011-12-09 2014-10-15 Butamax Advanced Biofuels LLC Process to remove product alcohols from fermentation broth
CN104321436A (zh) 2011-12-30 2015-01-28 布特马斯先进生物燃料有限责任公司 醇类的发酵生产
MX2014008073A (es) 2011-12-30 2014-10-06 Butamax Advanced Biofuels Llc Cambios geneticos para la produccion de butanol.
MX2014011355A (es) 2012-03-23 2014-12-05 Butamax Advanced Biofuels Llc Complementacion con acetato de medio para butanologenos.
KR20150014953A (ko) 2012-05-11 2015-02-09 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 케톨-산 리덕토아이소머라아제 효소 및 사용 방법
US20140024064A1 (en) 2012-07-23 2014-01-23 Butamax(Tm) Advanced Biofuels Llc Processes and systems for the production of fermentative alcohols
AU2013305714A1 (en) 2012-08-22 2015-02-05 Butamax Advanced Biofuels Llc Production of fermentation products
EP2895612A1 (en) 2012-09-12 2015-07-22 Butamax Advanced Biofuels LLC Processes and systems for the production of fermentation products
WO2014047421A1 (en) 2012-09-21 2014-03-27 Butamax(Tm) Advanced Biofuels Llc Production of renewable hydrocarbon compositions
CA2886157A1 (en) 2012-09-26 2014-04-03 Butamax Advanced Biofuels Llc Polypeptides with ketol-acid reductoisomerase activity
AU2013323396B2 (en) 2012-09-28 2017-04-20 Butamax Advanced Biofuels Llc Production of fermentation products
US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
BR112015008077A2 (pt) 2012-10-11 2017-12-05 Butamax Advanced Biofuels Llc métodos para a produção de um produto de fermentação
US9650624B2 (en) 2012-12-28 2017-05-16 Butamax Advanced Biofuels Llc DHAD variants for butanol production
WO2014105840A1 (en) 2012-12-31 2014-07-03 Butamax Advanced Biofuels Llc Fermentative production of alcohols
WO2014159309A1 (en) 2013-03-12 2014-10-02 Butamax Advanced Biofuels Llc Processes and systems for the production of alcohols
CA2905912A1 (en) 2013-03-14 2014-10-02 E. I. Du Pont De Nemours And Company Glycerol 3- phosphate dehydrogenase for butanol production
WO2014144643A1 (en) 2013-03-15 2014-09-18 Butamax Advanced Biofuels Llc Method for producing butanol using extractive fermentation
WO2014144728A1 (en) 2013-03-15 2014-09-18 Butamax Advanced Biofuels Llc Method for production of butanol using extractive fermentation
WO2014151645A1 (en) 2013-03-15 2014-09-25 Butamax Advanced Biofuels Llc Process for maximizing biomass growth and butanol yield by feedback control
WO2014151190A1 (en) 2013-03-15 2014-09-25 Butamax Advanced Biofuels Llc Dhad variants and methods of screening
WO2014144210A2 (en) 2013-03-15 2014-09-18 Butamax Advanced Biofuels Llc Competitive growth and/or production advantage for butanologen microorganism
WO2015002913A1 (en) 2013-07-03 2015-01-08 Butamax Advanced Biofuels Llc Partial adaptation for butanol production
WO2016025425A1 (en) 2014-08-11 2016-02-18 Butamax Advanced Biofuels Llc Yeast preparations and methods of making the same
EP3589740A1 (en) 2017-03-03 2020-01-08 NMR - Bio Cell-free synthesis of isotopic labelled proteins from amino-acids precursors
CN111893083A (zh) * 2019-05-05 2020-11-06 中国科学院上海高等研究院 改造的克雷伯氏属细菌及其生产2,3-二羟基异戊酸的应用
DE102022119024A1 (de) * 2022-07-28 2024-02-08 INM - Leibniz-Institut für Neue Materialien gemeinnützige Gesellschaft mit beschränkter Haftung Neuartige genetische Werkzeuge

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
FR2696190B1 (fr) * 1992-09-25 1994-12-09 Agronomique Inst Nat Rech Acide nucléique codant pour une alpha-acétolactate synthase et ses applications.
US6800744B1 (en) * 1997-07-02 2004-10-05 Genome Therapeutics Corporation Nucleic acid and amino acid sequences relating to Streptococcus pneumoniae for diagnostics and therapeutics
DE19855312A1 (de) * 1998-12-01 2000-06-08 Degussa Verfahren zur fermentativen Herstellung von D-Pantothensäure unter Verwendung coryneformer Bakterien
EP1887081A2 (en) * 1999-02-25 2008-02-13 Ceres Incorporated DNA Sequences
US7273769B1 (en) 2000-08-16 2007-09-25 Micron Technology, Inc. Method and apparatus for removing encapsulating material from a packaged microelectronic device
AU2002237676A1 (en) * 2000-11-22 2002-06-03 Cargill Dow Polymers, Llc Methods and materials for the synthesis of organic products
US20110081348A1 (en) 2004-12-01 2011-04-07 Nina Brogden Fungal signalling and metabolic enzymes
JP5804666B2 (ja) 2005-04-12 2015-11-04 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ バイオマスの処理および利用における別の供給流れの集中
CA2617734A1 (en) * 2005-08-12 2007-02-22 Suntory Limited Dihydroxy-acid dehydratase gene and use thereof
BRPI0619325B8 (pt) 2005-10-26 2020-11-17 Butamax Advanced Biofuels Llc célula hospedeira microbiana recombinante e método para produção de isobutanol
CN101442910A (zh) 2006-03-13 2009-05-27 卡吉尔公司 具有从磷酸二羟丙酮到甘油的途径被破坏的酵母细胞
US8962298B2 (en) * 2006-05-02 2015-02-24 Butamax Advanced Biofuels Llc Recombinant host cell comprising a diol dehydratase
JP2011510611A (ja) 2007-02-09 2011-04-07 ザ レジェンツ オブ ザ ユニヴァースティ オブ カリフォルニア 組換え微生物によるバイオ燃料の生成
BRPI0808600A2 (pt) 2007-04-18 2018-12-26 Du Pont "método para a conversão de acetolactato em dihidroxi-isovalerato, método para a produção de isobutanol, célula hospedeira recombinante e método para a identificação e isolameno de uma construção genética"
CA2990939A1 (en) 2007-12-23 2009-07-09 Gevo, Inc. Yeast organism producing isobutanol at a high yield
DE102008010121B4 (de) 2008-02-20 2013-11-21 Butalco Gmbh Fermentative Produktion von Isobutanol mit Hefe
EP2283141B1 (en) * 2008-06-04 2014-04-23 Butamax (TM) Advanced Biofuels LLC A method for producing butanol using extraction and gas stripping
BRPI0909989A2 (pt) * 2008-06-05 2021-06-22 Butamax Advanced Biofuels Llc célula de levedura recombinante e método para a produção de um produto
EP2337869B1 (en) 2008-09-29 2015-08-19 Butamax Advanced Biofuels Llc Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
WO2010037105A1 (en) 2008-09-29 2010-04-01 Butamax™ Advanced Biofuels LLC Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria
WO2010037111A1 (en) 2008-09-29 2010-04-01 Butamax™ Advanced Biofuels LLC INCREASED HETEROLOGOUS Fe-S ENZYME ACTIIVTY IN YEAST
EP2342330A1 (en) 2008-09-29 2011-07-13 Butamax Advanced Biofuels Llc Enhanced iron-sulfur cluster formation for increased dihydroxy-acid dehydratase activity in lactic acid bacteria
EP2337851B1 (en) 2008-09-29 2014-08-13 Butamax Advanced Biofuels Llc IDENTIFICATION AND USE OF BACTERIAL Ý2Fe-2S¨DIHYDROXY-ACID DEHYDRATASES
US8828694B2 (en) * 2008-11-13 2014-09-09 Butamax Advanced Biofuels Llc Production of isobutanol in yeast mitochondria
BR112012003241A2 (pt) * 2009-08-12 2015-09-01 Gevo Inc Localização de via de isobutanol citosólico para a produção de isobutanol
IN2012DN02227A (enExample) * 2009-09-29 2015-08-21 Butamax Tm Advanced Biofuels
US9260708B2 (en) * 2009-09-29 2016-02-16 Butamax Advanced Biofuels Llc Yeast production host cells
EP2504447A2 (en) * 2009-11-23 2012-10-03 ButamaxTM Advanced Biofuels LLC Method for producing butanol using extractive fermentation with osmolyte addition
CN102656272A (zh) * 2009-11-23 2012-09-05 布特马斯先进生物燃料有限责任公司 使用添加电解质的萃取发酵来生产丁醇的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FLINT, D. H., et al., The Journal of Biological Chemistry, 1988, vol. 263, pages 3558-3564 *

Also Published As

Publication number Publication date
EP2821484A1 (en) 2015-01-07
MX2011003238A (es) 2011-04-28
CA2735690A1 (en) 2010-04-01
KR101659101B1 (ko) 2016-09-22
AU2009296225A1 (en) 2010-04-01
US20140030776A1 (en) 2014-01-30
EP2337851A1 (en) 2011-06-29
EP2821484B1 (en) 2016-06-29
ES2521675T3 (es) 2014-11-13
EP2337851B1 (en) 2014-08-13
US20140051137A1 (en) 2014-02-20
ZA201101367B (en) 2014-03-26
US8951937B2 (en) 2015-02-10
NZ591244A (en) 2013-03-28
US20100081154A1 (en) 2010-04-01
KR20110063575A (ko) 2011-06-10
CN102186973B (zh) 2017-08-15
WO2010037112A1 (en) 2010-04-01
CN102186973A (zh) 2011-09-14
BRPI0913682A2 (pt) 2020-08-11
ZA201309658B (en) 2016-06-29
JP5846912B2 (ja) 2016-01-20
JP2012503993A (ja) 2012-02-16

Similar Documents

Publication Publication Date Title
AU2009296225B2 (en) Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases
US9909149B2 (en) DHAD variants for butanol production
US10287566B2 (en) DHAD variants and methods of screening
US8637281B2 (en) Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria
US8828694B2 (en) Production of isobutanol in yeast mitochondria
EP2737073B1 (en) Keto-isovalerate decarboxylase enzymes and methods of use thereof
US8241878B2 (en) Recombinant yeast host cell with Fe-S cluster proteins and methods of using thereof
MX2011003272A (es) Polipeptidos de eritropoyetina animal modificados y sus usos.
US20100081182A1 (en) Enhanced iron-sulfur cluster formation for increased dihydroxy-acid dehydratase activity in lactic acid bacteria
US20160222370A1 (en) Recombinant Yeast Host Cell With Fe-S Cluster Proteins And Methods Of Using Thereof
NZ717195B2 (en) Keto-isovalerate decarboxylase enzymes and methods of use thereof

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)