ZA200707216B - Replication-deficient RNA viruses as vaccines - Google Patents
Replication-deficient RNA viruses as vaccines Download PDFInfo
- Publication number
- ZA200707216B ZA200707216B ZA200707216A ZA200707216A ZA200707216B ZA 200707216 B ZA200707216 B ZA 200707216B ZA 200707216 A ZA200707216 A ZA 200707216A ZA 200707216 A ZA200707216 A ZA 200707216A ZA 200707216 B ZA200707216 B ZA 200707216B
- Authority
- ZA
- South Africa
- Prior art keywords
- virus
- cell
- egfp
- negative
- protein
- Prior art date
Links
- 230000010076 replication Effects 0.000 title claims abstract description 67
- 241001493065 dsRNA viruses Species 0.000 title claims abstract description 47
- 229960005486 vaccine Drugs 0.000 title claims abstract description 35
- 230000002950 deficient Effects 0.000 title description 47
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 139
- 238000013518 transcription Methods 0.000 claims abstract description 75
- 230000035897 transcription Effects 0.000 claims abstract description 75
- 230000003612 virological effect Effects 0.000 claims abstract description 41
- 108020004414 DNA Proteins 0.000 claims abstract description 37
- 101710177166 Phosphoprotein Proteins 0.000 claims abstract description 37
- 102100034574 P protein Human genes 0.000 claims abstract description 33
- 101710181008 P protein Proteins 0.000 claims abstract description 31
- 230000035772 mutation Effects 0.000 claims abstract description 24
- 102000053602 DNA Human genes 0.000 claims abstract description 18
- 101710141454 Nucleoprotein Proteins 0.000 claims abstract description 14
- 230000000694 effects Effects 0.000 claims abstract description 8
- 108090000994 Catalytic RNA Proteins 0.000 claims abstract description 4
- 102000053642 Catalytic RNA Human genes 0.000 claims abstract description 4
- 108091092562 ribozyme Proteins 0.000 claims abstract description 4
- 210000004027 cell Anatomy 0.000 claims description 208
- 241000700605 Viruses Species 0.000 claims description 162
- 241000711408 Murine respirovirus Species 0.000 claims description 69
- 102000004169 proteins and genes Human genes 0.000 claims description 61
- 238000004519 manufacturing process Methods 0.000 claims description 50
- 239000013598 vector Substances 0.000 claims description 32
- 150000001413 amino acids Chemical class 0.000 claims description 20
- 208000015181 infectious disease Diseases 0.000 claims description 19
- 102000036639 antigens Human genes 0.000 claims description 18
- 239000000427 antigen Substances 0.000 claims description 17
- 108091007433 antigens Proteins 0.000 claims description 17
- 238000012217 deletion Methods 0.000 claims description 16
- 230000037430 deletion Effects 0.000 claims description 14
- 239000008194 pharmaceutical composition Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 9
- 230000002829 reductive effect Effects 0.000 claims description 8
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims description 7
- 230000036961 partial effect Effects 0.000 claims description 7
- 244000052769 pathogen Species 0.000 claims description 7
- 230000001717 pathogenic effect Effects 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 230000029812 viral genome replication Effects 0.000 claims description 7
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims description 6
- 230000001419 dependent effect Effects 0.000 claims description 5
- 210000004962 mammalian cell Anatomy 0.000 claims description 5
- 230000001225 therapeutic effect Effects 0.000 claims description 5
- 210000004779 membrane envelope Anatomy 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 108010003533 Viral Envelope Proteins Proteins 0.000 claims description 3
- 239000013543 active substance Substances 0.000 claims description 3
- 229940031348 multivalent vaccine Drugs 0.000 claims description 3
- 238000002560 therapeutic procedure Methods 0.000 claims description 3
- 230000000259 anti-tumor effect Effects 0.000 claims description 2
- 229940031346 monovalent vaccine Drugs 0.000 claims description 2
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 2
- 241001515965 unidentified phage Species 0.000 claims description 2
- 108020004459 Small interfering RNA Proteins 0.000 claims 1
- 208000036142 Viral infection Diseases 0.000 claims 1
- 230000000692 anti-sense effect Effects 0.000 claims 1
- 230000009385 viral infection Effects 0.000 claims 1
- 101150084044 P gene Proteins 0.000 abstract description 7
- 101001065501 Escherichia phage MS2 Lysis protein Proteins 0.000 abstract description 5
- 108091034117 Oligonucleotide Proteins 0.000 abstract description 4
- 230000009368 gene silencing by RNA Effects 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 3
- 101150062031 L gene Proteins 0.000 abstract description 2
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 abstract description 2
- 230000001939 inductive effect Effects 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 2
- 108091030071 RNAI Proteins 0.000 abstract 1
- 239000000074 antisense oligonucleotide Substances 0.000 abstract 1
- 238000012230 antisense oligonucleotides Methods 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 230000010534 mechanism of action Effects 0.000 abstract 1
- 239000012873 virucide Substances 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 53
- 230000014509 gene expression Effects 0.000 description 45
- 210000003501 vero cell Anatomy 0.000 description 45
- 108010048367 enhanced green fluorescent protein Proteins 0.000 description 38
- 108700019146 Transgenes Proteins 0.000 description 27
- 239000002245 particle Substances 0.000 description 24
- 239000000047 product Substances 0.000 description 22
- 239000002299 complementary DNA Substances 0.000 description 21
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 20
- 108700026244 Open Reading Frames Proteins 0.000 description 17
- 230000015572 biosynthetic process Effects 0.000 description 17
- 238000010367 cloning Methods 0.000 description 16
- 101710133291 Hemagglutinin-neuraminidase Proteins 0.000 description 15
- 239000012634 fragment Substances 0.000 description 14
- 210000002443 helper t lymphocyte Anatomy 0.000 description 13
- 108020004999 messenger RNA Proteins 0.000 description 13
- 241000282414 Homo sapiens Species 0.000 description 12
- 230000006870 function Effects 0.000 description 12
- 238000003786 synthesis reaction Methods 0.000 description 12
- 238000012360 testing method Methods 0.000 description 12
- 238000011534 incubation Methods 0.000 description 10
- 210000003743 erythrocyte Anatomy 0.000 description 9
- 230000028993 immune response Effects 0.000 description 8
- 230000001965 increasing effect Effects 0.000 description 8
- 239000002773 nucleotide Substances 0.000 description 8
- 125000003729 nucleotide group Chemical group 0.000 description 8
- 101000757797 Geobacillus stearothermophilus Aminopeptidase 2 Proteins 0.000 description 7
- 101000733766 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Aminopeptidase 2, mitochondrial Proteins 0.000 description 7
- 241000700618 Vaccinia virus Species 0.000 description 7
- 108010067390 Viral Proteins Proteins 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 238000003556 assay Methods 0.000 description 7
- 238000003780 insertion Methods 0.000 description 7
- 230000037431 insertion Effects 0.000 description 7
- 238000011835 investigation Methods 0.000 description 7
- 239000013612 plasmid Substances 0.000 description 7
- 238000002255 vaccination Methods 0.000 description 7
- 239000013603 viral vector Substances 0.000 description 7
- 208000002606 Paramyxoviridae Infections Diseases 0.000 description 6
- 230000027455 binding Effects 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 6
- 230000000295 complement effect Effects 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- 230000002441 reversible effect Effects 0.000 description 6
- 230000014616 translation Effects 0.000 description 6
- 238000001262 western blot Methods 0.000 description 6
- 108020004705 Codon Proteins 0.000 description 5
- 108010052285 Membrane Proteins Proteins 0.000 description 5
- 102000018697 Membrane Proteins Human genes 0.000 description 5
- 108020000999 Viral RNA Proteins 0.000 description 5
- 230000007812 deficiency Effects 0.000 description 5
- 230000028996 humoral immune response Effects 0.000 description 5
- 230000006698 induction Effects 0.000 description 5
- 238000011002 quantification Methods 0.000 description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 230000002238 attenuated effect Effects 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000012894 fetal calf serum Substances 0.000 description 4
- 230000004927 fusion Effects 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000002243 precursor Substances 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 3
- 108010068327 4-hydroxyphenylpyruvate dioxygenase Proteins 0.000 description 3
- 208000003322 Coinfection Diseases 0.000 description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 3
- 108010089430 Phosphoproteins Proteins 0.000 description 3
- 102000007982 Phosphoproteins Human genes 0.000 description 3
- 241000711798 Rabies lyssavirus Species 0.000 description 3
- 241001068263 Replication competent viruses Species 0.000 description 3
- 108700008625 Reporter Genes Proteins 0.000 description 3
- 108091081024 Start codon Proteins 0.000 description 3
- 101710172711 Structural protein Proteins 0.000 description 3
- 210000001744 T-lymphocyte Anatomy 0.000 description 3
- 101710137500 T7 RNA polymerase Proteins 0.000 description 3
- 241000711975 Vesicular stomatitis virus Species 0.000 description 3
- 108700005077 Viral Genes Proteins 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 230000001086 cytosolic effect Effects 0.000 description 3
- 210000003527 eukaryotic cell Anatomy 0.000 description 3
- 210000000987 immune system Anatomy 0.000 description 3
- 230000003053 immunization Effects 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 230000003902 lesion Effects 0.000 description 3
- 230000016379 mucosal immune response Effects 0.000 description 3
- 231100000219 mutagenic Toxicity 0.000 description 3
- 230000003505 mutagenic effect Effects 0.000 description 3
- 230000003472 neutralizing effect Effects 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 238000013492 plasmid preparation Methods 0.000 description 3
- 238000001243 protein synthesis Methods 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 230000000241 respiratory effect Effects 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 238000013207 serial dilution Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- 238000012795 verification Methods 0.000 description 3
- 241000712005 Bovine respirovirus 3 Species 0.000 description 2
- 241000282552 Chlorocebus aethiops Species 0.000 description 2
- 241000712003 Human respirovirus 3 Species 0.000 description 2
- 108091029795 Intergenic region Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 239000002033 PVDF binder Substances 0.000 description 2
- 241000711504 Paramyxoviridae Species 0.000 description 2
- 108010015724 Prephenate Dehydratase Proteins 0.000 description 2
- 229940022005 RNA vaccine Drugs 0.000 description 2
- 241000711931 Rhabdoviridae Species 0.000 description 2
- 239000008186 active pharmaceutical agent Substances 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 230000000890 antigenic effect Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 229940031416 bivalent vaccine Drugs 0.000 description 2
- 210000004899 c-terminal region Anatomy 0.000 description 2
- 230000032823 cell division Effects 0.000 description 2
- 230000006790 cellular biosynthetic process Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000003184 complementary RNA Substances 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000013613 expression plasmid Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 2
- 108010021843 fluorescent protein 583 Proteins 0.000 description 2
- 108091006047 fluorescent proteins Proteins 0.000 description 2
- 102000034287 fluorescent proteins Human genes 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 108700021021 mRNA Vaccine Proteins 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 238000010172 mouse model Methods 0.000 description 2
- 239000013600 plasmid vector Substances 0.000 description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 210000001236 prokaryotic cell Anatomy 0.000 description 2
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
- 230000006648 viral gene expression Effects 0.000 description 2
- 238000000316 virotherapy Methods 0.000 description 2
- 101150066375 35 gene Proteins 0.000 description 1
- 101150005197 5.5 gene Proteins 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- 241000712892 Arenaviridae Species 0.000 description 1
- 241000711404 Avian avulavirus 1 Species 0.000 description 1
- 241000537222 Betabaculovirus Species 0.000 description 1
- 241000776207 Bornaviridae Species 0.000 description 1
- 102100023701 C-C motif chemokine 18 Human genes 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 108020004394 Complementary RNA Proteins 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- UHDGCWIWMRVCDJ-CCXZUQQUSA-N Cytarabine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O1 UHDGCWIWMRVCDJ-CCXZUQQUSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 101150034814 F gene Proteins 0.000 description 1
- 241000711950 Filoviridae Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108060003393 Granulin Proteins 0.000 description 1
- 101150008820 HN gene Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000978371 Homo sapiens C-C motif chemokine 18 Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 108010002616 Interleukin-5 Proteins 0.000 description 1
- 241000282560 Macaca mulatta Species 0.000 description 1
- 101710085938 Matrix protein Proteins 0.000 description 1
- 241000712079 Measles morbillivirus Species 0.000 description 1
- 101710127721 Membrane protein Proteins 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 241000711386 Mumps virus Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000005348 Neuraminidase Human genes 0.000 description 1
- 108010006232 Neuraminidase Proteins 0.000 description 1
- 108091093105 Nuclear DNA Proteins 0.000 description 1
- 102000011931 Nucleoproteins Human genes 0.000 description 1
- 108010061100 Nucleoproteins Proteins 0.000 description 1
- 101150096919 P/C gene Proteins 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 241000150350 Peribunyaviridae Species 0.000 description 1
- 108010065868 RNA polymerase SP6 Proteins 0.000 description 1
- 206010037742 Rabies Diseases 0.000 description 1
- 108020005091 Replication Origin Proteins 0.000 description 1
- 241000725643 Respiratory syncytial virus Species 0.000 description 1
- 108700002730 Sendai virus L Proteins 0.000 description 1
- 101710126110 Surface protein F Proteins 0.000 description 1
- 102100023935 Transmembrane glycoprotein NMB Human genes 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- 206010058874 Viraemia Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000004163 cytometry Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000005090 green fluorescent protein Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 230000002685 pulmonary effect Effects 0.000 description 1
- 229960003127 rabies vaccine Drugs 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 210000004739 secretory vesicle Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000010473 stable expression Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 108091007466 transmembrane glycoproteins Proteins 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 229940031418 trivalent vaccine Drugs 0.000 description 1
- 230000006490 viral transcription Effects 0.000 description 1
- 230000029302 virus maturation Effects 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C07K14/08—RNA viruses
- C07K14/115—Paramyxoviridae, e.g. parainfluenza virus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18811—Sendai virus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/36011—Togaviridae
- C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
- C12N2770/36141—Use of virus, viral particle or viral elements as a vector
- C12N2770/36143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Epidemiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102005006388A DE102005006388A1 (de) | 2005-02-11 | 2005-02-11 | Replikationsdefiziente RNA-Viren als Impfstoffe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA200707216B true ZA200707216B (en) | 2008-05-28 |
Family
ID=36353666
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA200707216A ZA200707216B (en) | 2005-02-11 | 2007-08-27 | Replication-deficient RNA viruses as vaccines |
Country Status (23)
| Country | Link |
|---|---|
| US (2) | US20090041725A1 (da) |
| EP (2) | EP1851239B1 (da) |
| JP (2) | JP5679620B2 (da) |
| KR (1) | KR101279636B1 (da) |
| CN (1) | CN101155826B (da) |
| AT (1) | ATE421529T1 (da) |
| AU (1) | AU2006212385B2 (da) |
| BR (1) | BRPI0607487A2 (da) |
| CA (1) | CA2599036C (da) |
| DE (2) | DE102005006388A1 (da) |
| DK (1) | DK1851239T3 (da) |
| EA (1) | EA015013B1 (da) |
| ES (1) | ES2321651T3 (da) |
| HK (1) | HK1118297A1 (da) |
| HR (1) | HRP20090230T1 (da) |
| IL (1) | IL185147A (da) |
| MX (1) | MX2007009628A (da) |
| PL (1) | PL1851239T3 (da) |
| PT (1) | PT1851239E (da) |
| RS (1) | RS50977B (da) |
| SI (1) | SI1851239T1 (da) |
| WO (1) | WO2006084746A1 (da) |
| ZA (1) | ZA200707216B (da) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005006388A1 (de) | 2005-02-11 | 2006-08-24 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Replikationsdefiziente RNA-Viren als Impfstoffe |
| HRP20221475T3 (hr) | 2007-12-27 | 2023-01-06 | Universität Zürich | Replikacijski defektni vektori arenavirusa |
| EP2085479A1 (en) * | 2008-01-31 | 2009-08-05 | Institut Pasteur | Reverse genetics of negative-strand rna viruses in yeast |
| JP5633075B2 (ja) * | 2009-05-18 | 2014-12-03 | 独立行政法人産業技術総合研究所 | 多能性幹細胞作成用ベクター材料及びこれを用いた多能性幹細胞作成方法 |
| US9365866B2 (en) | 2009-06-03 | 2016-06-14 | National Institute Of Advanced Industrial Science And Technology | Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same |
| DE102010018961B4 (de) * | 2010-04-23 | 2012-09-20 | Eberhard-Karls-Universität Tübingen Universitätsklinikum | Genetisch modifiziertes Paramyxovirus zur Behandlung von Tumorerkrankungen |
| ES2640113T3 (es) | 2011-02-08 | 2017-10-31 | Mie University | Método para producir un vector viral para la transferencia génica |
| JP6757121B2 (ja) * | 2011-10-05 | 2020-09-16 | ジェンヴェック エルエルシー | シミアン（ゴリラ）アデノウイルス又はアデノウイルスベクター、及び使用方法 |
| EP2669381A1 (en) * | 2012-05-30 | 2013-12-04 | AmVac AG | Method for expression of heterologous proteins using a recombinant negative-strand RNA virus vector comprising a mutated P protein |
| EP2968506B1 (en) | 2013-03-15 | 2019-07-31 | Université de Genève | Anti-mycobacterial vaccines |
| EP2813574B1 (en) * | 2013-06-10 | 2019-02-20 | RSV Genius GmbH | Semi-live respiratory syncytial virus vaccine |
| WO2014198407A1 (en) * | 2013-06-10 | 2014-12-18 | Amvac Ag | Semi-live respiratory syncytial virus vaccine |
| WO2023227758A1 (en) | 2022-05-25 | 2023-11-30 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Vaccine with reduced anti-vector antigenicity |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0702085T4 (da) | 1994-07-18 | 2010-04-06 | Conzelmann Karl Klaus Prof Dr | Rekombinant infektiøs ikke-segmenteret negativ-strenget RNA-virus |
| WO1997016539A1 (fr) * | 1995-11-01 | 1997-05-09 | Dnavec Research Inc. | Virus sendai recombinant |
| AU785148B2 (en) | 1999-12-10 | 2006-10-05 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Use of recombinant parainfluenza viruses (PIVs) as vectors to protect against infection and disease caused by PIV and other human pathogens |
| JP4791651B2 (ja) * | 2000-05-18 | 2011-10-12 | 株式会社ディナベック研究所 | 外来遺伝子導入用パラミクソウイルスベクター |
| KR20040039366A (ko) * | 2001-09-18 | 2004-05-10 | 가부시키가이샤 디나벡크 겐큐쇼 | 입자 형성능이 저하된 （－）가닥 ｒｎａ 바이러스 벡터의 검사방법 및 제조방법 |
| JP2004344001A (ja) * | 2003-03-07 | 2004-12-09 | Dnavec Research Inc | 胚性幹細胞に遺伝子を導入する方法 |
| JP2004357689A (ja) * | 2003-06-03 | 2004-12-24 | Dnavec Research Inc | 遺伝子導入ベクターの非侵襲的インビボ評価方法 |
| KR101020532B1 (ko) * | 2003-06-09 | 2011-03-09 | 와이어쓰 엘엘씨 | ｃＤＮＡ로부터의 비분절 네가티브 쇄 ＲＮＡ 바이러스의 회수를 위한 개선된 방법 |
| DE102005006388A1 (de) | 2005-02-11 | 2006-08-24 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Replikationsdefiziente RNA-Viren als Impfstoffe |
-
2005
- 2005-02-11 DE DE102005006388A patent/DE102005006388A1/de not_active Withdrawn
-
2006
- 2006-02-10 EA EA200701695A patent/EA015013B1/ru not_active IP Right Cessation
- 2006-02-10 EP EP06706871A patent/EP1851239B1/de active Active
- 2006-02-10 AT AT06706871T patent/ATE421529T1/de active
- 2006-02-10 PT PT06706871T patent/PT1851239E/pt unknown
- 2006-02-10 CA CA2599036A patent/CA2599036C/en not_active Expired - Fee Related
- 2006-02-10 CN CN2006800090622A patent/CN101155826B/zh active Active
- 2006-02-10 AU AU2006212385A patent/AU2006212385B2/en not_active Ceased
- 2006-02-10 PL PL06706871T patent/PL1851239T3/pl unknown
- 2006-02-10 JP JP2007554510A patent/JP5679620B2/ja not_active Expired - Fee Related
- 2006-02-10 DE DE502006002711T patent/DE502006002711D1/de active Active
- 2006-02-10 WO PCT/EP2006/001251 patent/WO2006084746A1/de active Application Filing
- 2006-02-10 ES ES06706871T patent/ES2321651T3/es active Active
- 2006-02-10 BR BRPI0607487-1A patent/BRPI0607487A2/pt not_active Application Discontinuation
- 2006-02-10 MX MX2007009628A patent/MX2007009628A/es active IP Right Grant
- 2006-02-10 DK DK06706871T patent/DK1851239T3/da active
- 2006-02-10 KR KR1020077020606A patent/KR101279636B1/ko active IP Right Grant
- 2006-02-10 EP EP09000617A patent/EP2045260A1/de not_active Withdrawn
- 2006-02-10 SI SI200630270T patent/SI1851239T1/sl unknown
- 2006-02-10 RS RSP-2009/0185A patent/RS50977B/sr unknown
-
2007
- 2007-08-09 IL IL185147A patent/IL185147A/en not_active IP Right Cessation
- 2007-08-09 US US11/836,322 patent/US20090041725A1/en not_active Abandoned
- 2007-08-27 ZA ZA200707216A patent/ZA200707216B/en unknown
-
2008
- 2008-08-20 HK HK08109296.4A patent/HK1118297A1/xx not_active IP Right Cessation
-
2009
- 2009-04-20 HR HR20090230T patent/HRP20090230T1/xx unknown
-
2012
- 2012-07-23 JP JP2012162993A patent/JP2012213407A/ja active Pending
-
2017
- 2017-05-25 US US15/605,568 patent/US11028409B2/en active Active
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11028409B2 (en) | Replication-deficient RNA viruses as vaccines | |
| JP5779226B2 (ja) | 水疱性口内炎ウイルスの相乗的弱毒化、そのベクター及びその免疫原性組成物 | |
| JP3816126B2 (ja) | 組換え伝染性非セグメント化陰性鎖ｒｎａウイルス | |
| JP4463481B2 (ja) | 機能的に欠失したゲノムを含むコロナウイルス様の粒子 | |
| KR100912338B1 (ko) | 재조합 ｒｓｖ 바이러스 발현 시스템 및 백신 | |
| KR101020532B1 (ko) | ｃＤＮＡ로부터의 비분절 네가티브 쇄 ＲＮＡ 바이러스의 회수를 위한 개선된 방법 | |
| JP2003527122A (ja) | 組換えパラインフルエンザウイルス発現系とワクチン | |
| JP2003516148A (ja) | パラインフルエンザウィルス（ｐｉｖ）及びヒトの病原体により引き起こされる感染及び疾病を保護するためへの組換えｐｉｖのベクターとしての使用 | |
| KR100940776B1 (ko) | 약독화된 사람-소 키메라 파라인플루엔자바이러스(ｐｉｖ) 백신 | |
| US7041489B2 (en) | Recombinant respiratory syncytial viruses with deleted surface glycoprotein genes and uses thereof | |
| US20160120974A1 (en) | Semi-live respiratory syncytial virus vaccine | |
| Skiadopoulos et al. | Long nucleotide insertions between the HN and L protein coding regions of human parainfluenza virus type 3 yield viruses with temperature-sensitive and attenuation phenotypes | |
| EP3865180A1 (en) | Live recombinant measles virus expressing coronavirus antigens - its use in eliciting immunity against coronaviruses | |
| US12053518B2 (en) | Method for rescuing and producing a virus in avian cells | |
| US20230203536A1 (en) | Coronavirus rna replicons and use thereof as vaccines | |
| Meyers | Check for updates Chapter 2 Self-Replicating RNA Derived from the Genomes of Positive-Strand RNA Viruses Gregor Meyers and Birke Andrea Tews |