ZA200604032B - High efficiency gene transfer and expression in mammaliar cells by a multiple transfection procedure of MAF sequences - Google Patents
High efficiency gene transfer and expression in mammaliar cells by a multiple transfection procedure of MAF sequences Download PDFInfo
- Publication number
- ZA200604032B ZA200604032B ZA200604032A ZA200604032A ZA200604032B ZA 200604032 B ZA200604032 B ZA 200604032B ZA 200604032 A ZA200604032 A ZA 200604032A ZA 200604032 A ZA200604032 A ZA 200604032A ZA 200604032 B ZA200604032 B ZA 200604032B
- Authority
- ZA
- South Africa
- Prior art keywords
- sequence
- mar
- purified
- dna
- isolated
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 134
- 108090000623 proteins and genes Proteins 0.000 title claims description 131
- 230000014509 gene expression Effects 0.000 title claims description 77
- 238000001890 transfection Methods 0.000 title claims description 68
- 238000012546 transfer Methods 0.000 title description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 138
- 108020004414 DNA Proteins 0.000 claims abstract description 97
- 239000002773 nucleotide Substances 0.000 claims abstract description 78
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 77
- 230000027455 binding Effects 0.000 claims abstract description 38
- 230000001965 increasing effect Effects 0.000 claims abstract description 30
- 230000014616 translation Effects 0.000 claims abstract description 28
- 230000000295 complement effect Effects 0.000 claims abstract description 25
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims abstract description 11
- 101710096438 DNA-binding protein Proteins 0.000 claims abstract description 9
- 210000004027 cell Anatomy 0.000 claims description 161
- 239000012634 fragment Substances 0.000 claims description 84
- 230000000694 effects Effects 0.000 claims description 56
- 102000004169 proteins and genes Human genes 0.000 claims description 50
- 108091023040 Transcription factor Proteins 0.000 claims description 42
- 102000040945 Transcription factor Human genes 0.000 claims description 39
- 108010077544 Chromatin Proteins 0.000 claims description 32
- 210000003483 chromatin Anatomy 0.000 claims description 32
- 239000013598 vector Substances 0.000 claims description 24
- 239000011159 matrix material Substances 0.000 claims description 20
- 238000004519 manufacturing process Methods 0.000 claims description 16
- 102100040731 Zinc finger protein 384 Human genes 0.000 claims description 14
- 230000008018 melting Effects 0.000 claims description 14
- 238000002844 melting Methods 0.000 claims description 14
- 238000005452 bending Methods 0.000 claims description 12
- 230000008569 process Effects 0.000 claims description 12
- 230000004568 DNA-binding Effects 0.000 claims description 10
- 102100026459 POU domain, class 3, transcription factor 2 Human genes 0.000 claims description 10
- -1 POUBF1 Proteins 0.000 claims description 8
- 101000572986 Homo sapiens POU domain, class 3, transcription factor 2 Proteins 0.000 claims description 7
- 230000009261 transgenic effect Effects 0.000 claims description 7
- 102100037914 Pituitary-specific positive transcription factor 1 Human genes 0.000 claims description 6
- 101100518995 Caenorhabditis elegans pax-3 gene Proteins 0.000 claims description 5
- 102100027581 Forkhead box protein P3 Human genes 0.000 claims description 5
- 102100028092 Homeobox protein Nkx-3.1 Human genes 0.000 claims description 5
- 101000861452 Homo sapiens Forkhead box protein P3 Proteins 0.000 claims description 5
- 101000578249 Homo sapiens Homeobox protein Nkx-3.1 Proteins 0.000 claims description 5
- 101001096159 Homo sapiens Pituitary-specific positive transcription factor 1 Proteins 0.000 claims description 5
- 101000819074 Homo sapiens Transcription factor GATA-4 Proteins 0.000 claims description 5
- 108700042694 Lhx3 Proteins 0.000 claims description 5
- 101100518997 Mus musculus Pax3 gene Proteins 0.000 claims description 5
- 102000006289 Transcription Factor TFIIA Human genes 0.000 claims description 5
- 108010083262 Transcription Factor TFIIA Proteins 0.000 claims description 5
- 102100021380 Transcription factor GATA-4 Human genes 0.000 claims description 5
- 230000022131 cell cycle Effects 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 102100030835 AT-rich interactive domain-containing protein 5B Human genes 0.000 claims description 4
- 101710186200 CCAAT/enhancer-binding protein Proteins 0.000 claims description 4
- 102000006277 CDX2 Transcription Factor Human genes 0.000 claims description 4
- 108010083123 CDX2 Transcription Factor Proteins 0.000 claims description 4
- 102100037060 Forkhead box protein D3 Human genes 0.000 claims description 4
- 102100035134 Forkhead box protein J2 Human genes 0.000 claims description 4
- 102100035120 Forkhead box protein L1 Human genes 0.000 claims description 4
- 102100027570 Forkhead box protein Q1 Human genes 0.000 claims description 4
- 102100036646 Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial Human genes 0.000 claims description 4
- 102100030087 Homeobox protein DLX-1 Human genes 0.000 claims description 4
- 102100027893 Homeobox protein Nkx-2.1 Human genes 0.000 claims description 4
- 101000792947 Homo sapiens AT-rich interactive domain-containing protein 5B Proteins 0.000 claims description 4
- 101001029308 Homo sapiens Forkhead box protein D3 Proteins 0.000 claims description 4
- 101001023352 Homo sapiens Forkhead box protein L1 Proteins 0.000 claims description 4
- 101000861406 Homo sapiens Forkhead box protein Q1 Proteins 0.000 claims description 4
- 101001072655 Homo sapiens Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial Proteins 0.000 claims description 4
- 101000864690 Homo sapiens Homeobox protein DLX-1 Proteins 0.000 claims description 4
- 101000632178 Homo sapiens Homeobox protein Nkx-2.1 Proteins 0.000 claims description 4
- 101000845269 Homo sapiens Transcription termination factor 1 Proteins 0.000 claims description 4
- 102000007354 PAX6 Transcription Factor Human genes 0.000 claims description 4
- 101150081664 PAX6 gene Proteins 0.000 claims description 4
- 101150051296 foxj2 gene Proteins 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 101100281515 Arabidopsis thaliana FOX1 gene Proteins 0.000 claims description 3
- 102100023033 Cyclic AMP-dependent transcription factor ATF-2 Human genes 0.000 claims description 3
- 101100380508 Homo sapiens ATF2 gene Proteins 0.000 claims description 3
- 102100035593 POU domain, class 2, transcription factor 1 Human genes 0.000 claims description 3
- 101710084414 POU domain, class 2, transcription factor 1 Proteins 0.000 claims description 3
- 101150054854 POU1F1 gene Proteins 0.000 claims description 3
- 101150027852 pou3f2 gene Proteins 0.000 claims description 3
- 101150056399 slc20a1 gene Proteins 0.000 claims description 3
- 108700020911 DNA-Binding Proteins Proteins 0.000 claims description 2
- 108010068250 Herpes Simplex Virus Protein Vmw65 Proteins 0.000 claims description 2
- 102100032881 DNA-binding protein SATB1 Human genes 0.000 claims 2
- 101000655234 Homo sapiens DNA-binding protein SATB1 Proteins 0.000 claims 2
- 101100456626 Homo sapiens MEF2A gene Proteins 0.000 claims 2
- 101000964718 Homo sapiens Zinc finger protein 384 Proteins 0.000 claims 2
- 101100079042 Mus musculus Myef2 gene Proteins 0.000 claims 2
- 102100021148 Myocyte-specific enhancer factor 2A Human genes 0.000 claims 2
- 101150014102 mef-2 gene Proteins 0.000 claims 2
- 108010038661 Hepatocyte Nuclear Factor 3-alpha Proteins 0.000 claims 1
- 102000010818 Hepatocyte Nuclear Factor 3-alpha Human genes 0.000 claims 1
- 101150098999 pax8 gene Proteins 0.000 claims 1
- 241000282414 Homo sapiens Species 0.000 description 60
- 239000013612 plasmid Substances 0.000 description 56
- 210000000349 chromosome Anatomy 0.000 description 44
- 108700019146 Transgenes Proteins 0.000 description 33
- 238000004458 analytical method Methods 0.000 description 28
- 241000699666 Mus <mouse, genus> Species 0.000 description 20
- 238000009826 distribution Methods 0.000 description 17
- 230000000875 corresponding effect Effects 0.000 description 16
- 230000001105 regulatory effect Effects 0.000 description 15
- 239000003623 enhancer Substances 0.000 description 13
- 241000699670 Mus sp. Species 0.000 description 12
- 101710146690 Zinc finger protein 384 Proteins 0.000 description 12
- 230000002759 chromosomal effect Effects 0.000 description 12
- 238000005534 hematocrit Methods 0.000 description 12
- 210000000299 nuclear matrix Anatomy 0.000 description 12
- 102000008297 Nuclear Matrix-Associated Proteins Human genes 0.000 description 11
- 108010035916 Nuclear Matrix-Associated Proteins Proteins 0.000 description 11
- 150000007523 nucleic acids Chemical class 0.000 description 11
- 108090000394 Erythropoietin Proteins 0.000 description 10
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 10
- 210000003527 eukaryotic cell Anatomy 0.000 description 10
- 102000003951 Erythropoietin Human genes 0.000 description 9
- 241000287828 Gallus gallus Species 0.000 description 9
- 108010047956 Nucleosomes Proteins 0.000 description 9
- 229940105423 erythropoietin Drugs 0.000 description 9
- 230000010354 integration Effects 0.000 description 9
- 102000039446 nucleic acids Human genes 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 9
- 210000001623 nucleosome Anatomy 0.000 description 9
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 9
- 238000003556 assay Methods 0.000 description 8
- 210000003292 kidney cell Anatomy 0.000 description 8
- 239000003550 marker Substances 0.000 description 8
- 238000003752 polymerase chain reaction Methods 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- 230000006870 function Effects 0.000 description 7
- 238000001727 in vivo Methods 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 238000003151 transfection method Methods 0.000 description 7
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 210000004962 mammalian cell Anatomy 0.000 description 6
- RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical compound C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 description 6
- 238000013518 transcription Methods 0.000 description 6
- 230000035897 transcription Effects 0.000 description 6
- 241000282693 Cercopithecidae Species 0.000 description 5
- 102000014150 Interferons Human genes 0.000 description 5
- 108010050904 Interferons Proteins 0.000 description 5
- 230000003115 biocidal effect Effects 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 5
- 238000012217 deletion Methods 0.000 description 5
- 229960003722 doxycycline Drugs 0.000 description 5
- XQTWDDCIUJNLTR-CVHRZJFOSA-N doxycycline monohydrate Chemical compound O.O=C1C2=C(O)C=CC=C2[C@H](C)[C@@H]2C1=C(O)[C@]1(O)C(=O)C(C(N)=O)=C(O)[C@@H](N(C)C)[C@@H]1[C@H]2O XQTWDDCIUJNLTR-CVHRZJFOSA-N 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 239000013604 expression vector Substances 0.000 description 5
- 230000006801 homologous recombination Effects 0.000 description 5
- 238000002744 homologous recombination Methods 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 230000006698 induction Effects 0.000 description 5
- 229940079322 interferon Drugs 0.000 description 5
- 230000006798 recombination Effects 0.000 description 5
- 238000005215 recombination Methods 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 230000002103 transcriptional effect Effects 0.000 description 5
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 4
- 108010014251 Muramidase Proteins 0.000 description 4
- 241000699660 Mus musculus Species 0.000 description 4
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 4
- 240000006394 Sorghum bicolor Species 0.000 description 4
- 230000004913 activation Effects 0.000 description 4
- 230000001413 cellular effect Effects 0.000 description 4
- 230000037430 deletion Effects 0.000 description 4
- 102000034356 gene-regulatory proteins Human genes 0.000 description 4
- 108091006104 gene-regulatory proteins Proteins 0.000 description 4
- 229940088597 hormone Drugs 0.000 description 4
- 239000005556 hormone Substances 0.000 description 4
- 210000003917 human chromosome Anatomy 0.000 description 4
- 238000000126 in silico method Methods 0.000 description 4
- 239000012212 insulator Substances 0.000 description 4
- 210000003734 kidney Anatomy 0.000 description 4
- 210000005229 liver cell Anatomy 0.000 description 4
- 230000001404 mediated effect Effects 0.000 description 4
- 230000008520 organization Effects 0.000 description 4
- 230000003389 potentiating effect Effects 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 238000011830 transgenic mouse model Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 239000012097 Lipofectamine 2000 Substances 0.000 description 3
- 108010052285 Membrane Proteins Proteins 0.000 description 3
- 239000012124 Opti-MEM Substances 0.000 description 3
- 108010007568 Protamines Proteins 0.000 description 3
- 102000007327 Protamines Human genes 0.000 description 3
- 108700005075 Regulator Genes Proteins 0.000 description 3
- 108700008625 Reporter Genes Proteins 0.000 description 3
- 241000283984 Rodentia Species 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 230000001687 destabilization Effects 0.000 description 3
- 239000003651 drinking water Substances 0.000 description 3
- 235000020188 drinking water Nutrition 0.000 description 3
- 108010048367 enhanced green fluorescent protein Proteins 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000003102 growth factor Substances 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 210000003205 muscle Anatomy 0.000 description 3
- 210000004940 nucleus Anatomy 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 229940048914 protamine Drugs 0.000 description 3
- 229950010131 puromycin Drugs 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- 230000007704 transition Effects 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- 206010006187 Breast cancer Diseases 0.000 description 2
- 241000282465 Canis Species 0.000 description 2
- 241000282552 Chlorocebus aethiops Species 0.000 description 2
- 108091029523 CpG island Proteins 0.000 description 2
- 241000699800 Cricetinae Species 0.000 description 2
- 241000699802 Cricetulus griseus Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
- 108010034791 Heterochromatin Proteins 0.000 description 2
- 108010033040 Histones Proteins 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 108090000862 Ion Channels Proteins 0.000 description 2
- 102000004310 Ion Channels Human genes 0.000 description 2
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 2
- 108060001084 Luciferase Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241001529936 Murinae Species 0.000 description 2
- 102100031790 Myelin expression factor 2 Human genes 0.000 description 2
- 101710107751 Myelin expression factor 2 Proteins 0.000 description 2
- 206010035226 Plasma cell myeloma Diseases 0.000 description 2
- 241000700157 Rattus norvegicus Species 0.000 description 2
- 235000007230 Sorghum bicolor Nutrition 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- 101710172711 Structural protein Proteins 0.000 description 2
- 108091008874 T cell receptors Proteins 0.000 description 2
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 2
- 101710183280 Topoisomerase Proteins 0.000 description 2
- 244000000188 Vaccinium ovalifolium Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- MZVQCMJNVPIDEA-UHFFFAOYSA-N [CH2]CN(CC)CC Chemical group [CH2]CN(CC)CC MZVQCMJNVPIDEA-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000012190 activator Substances 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 238000003766 bioinformatics method Methods 0.000 description 2
- 208000019065 cervical carcinoma Diseases 0.000 description 2
- 238000012761 co-transfection Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000002224 dissection Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000013613 expression plasmid Substances 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 108091008053 gene clusters Proteins 0.000 description 2
- 239000005090 green fluorescent protein Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 210000004458 heterochromatin Anatomy 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 210000004408 hybridoma Anatomy 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 210000005265 lung cell Anatomy 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229960000485 methotrexate Drugs 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 201000000050 myeloid neoplasm Diseases 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 230000002085 persistent effect Effects 0.000 description 2
- 230000008488 polyadenylation Effects 0.000 description 2
- 238000001243 protein synthesis Methods 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 230000011218 segmentation Effects 0.000 description 2
- 210000000717 sertoli cell Anatomy 0.000 description 2
- 238000004114 suspension culture Methods 0.000 description 2
- 241000701161 unidentified adenovirus Species 0.000 description 2
- 241001430294 unidentified retrovirus Species 0.000 description 2
- 239000013603 viral vector Substances 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- IJJWOSAXNHWBPR-HUBLWGQQSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-n-(6-hydrazinyl-6-oxohexyl)pentanamide Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)NCCCCCC(=O)NN)SC[C@@H]21 IJJWOSAXNHWBPR-HUBLWGQQSA-N 0.000 description 1
- WFPZSXYXPSUOPY-ROYWQJLOSA-N ADP alpha-D-glucoside Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H]1O)O)N1C=2N=CN=C(C=2N=C1)N)OP(O)(=O)OP(O)(=O)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O WFPZSXYXPSUOPY-ROYWQJLOSA-N 0.000 description 1
- WFPZSXYXPSUOPY-UHFFFAOYSA-N ADP-mannose Natural products C1=NC=2C(N)=NC=NC=2N1C(C(C1O)O)OC1COP(O)(=O)OP(O)(=O)OC1OC(CO)C(O)C(O)C1O WFPZSXYXPSUOPY-UHFFFAOYSA-N 0.000 description 1
- 101710084907 AT-rich binding protein Proteins 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 102100027211 Albumin Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241000269350 Anura Species 0.000 description 1
- 101710095342 Apolipoprotein B Proteins 0.000 description 1
- 102100040202 Apolipoprotein B-100 Human genes 0.000 description 1
- 102000007592 Apolipoproteins Human genes 0.000 description 1
- 108010071619 Apolipoproteins Proteins 0.000 description 1
- 241000713842 Avian sarcoma virus Species 0.000 description 1
- 241000701822 Bovine papillomavirus Species 0.000 description 1
- 108010014064 CCCTC-Binding Factor Proteins 0.000 description 1
- 101100297347 Caenorhabditis elegans pgl-3 gene Proteins 0.000 description 1
- 240000001432 Calendula officinalis Species 0.000 description 1
- 235000005881 Calendula officinalis Nutrition 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 101000986346 Chironomus tentans High mobility group protein I Proteins 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 108010068426 Contractile Proteins Proteins 0.000 description 1
- 102000002585 Contractile Proteins Human genes 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 102000010831 Cytoskeletal Proteins Human genes 0.000 description 1
- 108010037414 Cytoskeletal Proteins Proteins 0.000 description 1
- 102000003844 DNA helicases Human genes 0.000 description 1
- 108090000133 DNA helicases Proteins 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 241000252212 Danio rerio Species 0.000 description 1
- 241000702421 Dependoparvovirus Species 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241000255601 Drosophila melanogaster Species 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 101150002621 EPO gene Proteins 0.000 description 1
- 102400001368 Epidermal growth factor Human genes 0.000 description 1
- 101800003838 Epidermal growth factor Proteins 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 102100031690 Erythroid transcription factor Human genes 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102100039556 Galectin-4 Human genes 0.000 description 1
- 101001035782 Gallus gallus Hemoglobin subunit beta Proteins 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 241000288105 Grus Species 0.000 description 1
- 102100021519 Hemoglobin subunit beta Human genes 0.000 description 1
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 1
- 241000700721 Hepatitis B virus Species 0.000 description 1
- 102100022057 Hepatocyte nuclear factor 1-alpha Human genes 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 102000006947 Histones Human genes 0.000 description 1
- 108010048671 Homeodomain Proteins Proteins 0.000 description 1
- 102000009331 Homeodomain Proteins Human genes 0.000 description 1
- 101001066268 Homo sapiens Erythroid transcription factor Proteins 0.000 description 1
- 101000608765 Homo sapiens Galectin-4 Proteins 0.000 description 1
- 101000897691 Homo sapiens Helix-loop-helix protein 1 Proteins 0.000 description 1
- 101001045751 Homo sapiens Hepatocyte nuclear factor 1-alpha Proteins 0.000 description 1
- 101001054334 Homo sapiens Interferon beta Proteins 0.000 description 1
- 101000602479 Homo sapiens Methionine-tRNA ligase, cytoplasmic Proteins 0.000 description 1
- 101000979342 Homo sapiens Nuclear factor NF-kappa-B p105 subunit Proteins 0.000 description 1
- 101000738966 Homo sapiens POU domain, class 6, transcription factor 1 Proteins 0.000 description 1
- 101000897669 Homo sapiens Small RNA 2'-O-methyltransferase Proteins 0.000 description 1
- 101000819111 Homo sapiens Trans-acting T-cell-specific transcription factor GATA-3 Proteins 0.000 description 1
- 108090000144 Human Proteins Proteins 0.000 description 1
- 102000003839 Human Proteins Human genes 0.000 description 1
- 241000701109 Human adenovirus 2 Species 0.000 description 1
- 241000701024 Human betaherpesvirus 5 Species 0.000 description 1
- GRRNUXAQVGOGFE-UHFFFAOYSA-N Hygromycin-B Natural products OC1C(NC)CC(N)C(O)C1OC1C2OC3(C(C(O)C(O)C(C(N)CO)O3)O)OC2C(O)C(CO)O1 GRRNUXAQVGOGFE-UHFFFAOYSA-N 0.000 description 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
- 108091029795 Intergenic region Proteins 0.000 description 1
- 108020003631 Kinetoplast DNA Proteins 0.000 description 1
- URLZCHNOLZSCCA-VABKMULXSA-N Leu-enkephalin Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)CNC(=O)CNC(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=CC=C1 URLZCHNOLZSCCA-VABKMULXSA-N 0.000 description 1
- 102000055120 MEF2 Transcription Factors Human genes 0.000 description 1
- 108010018650 MEF2 Transcription Factors Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 102000006890 Methyl-CpG-Binding Protein 2 Human genes 0.000 description 1
- 108010072388 Methyl-CpG-Binding Protein 2 Proteins 0.000 description 1
- 102000005431 Molecular Chaperones Human genes 0.000 description 1
- 108010006519 Molecular Chaperones Proteins 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000008763 Neurofilament Proteins Human genes 0.000 description 1
- 108010088373 Neurofilament Proteins Proteins 0.000 description 1
- 102100023050 Nuclear factor NF-kappa-B p105 subunit Human genes 0.000 description 1
- 102000043276 Oncogene Human genes 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 102000016978 Orphan receptors Human genes 0.000 description 1
- 108070000031 Orphan receptors Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102100037483 POU domain, class 6, transcription factor 1 Human genes 0.000 description 1
- 241001631646 Papillomaviridae Species 0.000 description 1
- 102000009097 Phosphorylases Human genes 0.000 description 1
- 108010073135 Phosphorylases Proteins 0.000 description 1
- 101710129981 Pituitary-specific positive transcription factor 1 Proteins 0.000 description 1
- 241001505332 Polyomavirus sp. Species 0.000 description 1
- 108010013381 Porins Proteins 0.000 description 1
- 102000017033 Porins Human genes 0.000 description 1
- 102000001253 Protein Kinase Human genes 0.000 description 1
- 102000052575 Proto-Oncogene Human genes 0.000 description 1
- 108700020978 Proto-Oncogene Proteins 0.000 description 1
- 101000945964 Rattus norvegicus CCAAT/enhancer-binding protein beta Proteins 0.000 description 1
- 101000924396 Rattus norvegicus Cytosol aminopeptidase Proteins 0.000 description 1
- 101001001280 Rattus norvegicus Lysosomal acid phosphatase Proteins 0.000 description 1
- 101100346194 Rattus norvegicus Mpc1 gene Proteins 0.000 description 1
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
- 101150032199 Rplp0 gene Proteins 0.000 description 1
- 101000884545 Schizosaccharomyces pombe (strain 972 / ATCC 24843) Pre-mRNA-splicing factor cdc5 Proteins 0.000 description 1
- 102100021887 Small RNA 2'-O-methyltransferase Human genes 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 102100038803 Somatotropin Human genes 0.000 description 1
- 208000002903 Thalassemia Diseases 0.000 description 1
- 102100021386 Trans-acting T-cell-specific transcription factor GATA-3 Human genes 0.000 description 1
- 102100027671 Transcriptional repressor CTCF Human genes 0.000 description 1
- 102000007537 Type II DNA Topoisomerases Human genes 0.000 description 1
- 108010046308 Type II DNA Topoisomerases Proteins 0.000 description 1
- 108090000848 Ubiquitin Proteins 0.000 description 1
- 102000044159 Ubiquitin Human genes 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000003281 allosteric effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 239000003098 androgen Substances 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000002051 biphasic effect Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 101150032707 burs gene Proteins 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000019113 chromatin silencing Effects 0.000 description 1
- 238000011098 chromatofocusing Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 238000012875 competitive assay Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 239000003246 corticosteroid Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229940116977 epidermal growth factor Drugs 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 108060003196 globin Proteins 0.000 description 1
- 102000018146 globin Human genes 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 230000006195 histone acetylation Effects 0.000 description 1
- 102000057346 human MARS1 Human genes 0.000 description 1
- GRRNUXAQVGOGFE-NZSRVPFOSA-N hygromycin B Chemical compound O[C@@H]1[C@@H](NC)C[C@@H](N)[C@H](O)[C@H]1O[C@H]1[C@H]2O[C@@]3([C@@H]([C@@H](O)[C@@H](O)[C@@H](C(N)CO)O3)O)O[C@H]2[C@@H](O)[C@@H](CO)O1 GRRNUXAQVGOGFE-NZSRVPFOSA-N 0.000 description 1
- 229940097277 hygromycin b Drugs 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000016507 interphase Effects 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 210000005075 mammary gland Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000031864 metaphase Effects 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000001114 myogenic effect Effects 0.000 description 1
- 210000001087 myotubule Anatomy 0.000 description 1
- 210000005044 neurofilament Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- 210000004492 nuclear pore Anatomy 0.000 description 1
- 230000006911 nucleation Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 238000002888 pairwise sequence alignment Methods 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 210000001322 periplasm Anatomy 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000001817 pituitary effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000004007 reversed phase HPLC Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000007423 screening assay Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 208000007056 sickle cell anemia Diseases 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 108020001568 subdomains Proteins 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 239000012096 transfection reagent Substances 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 102000027257 transmembrane receptors Human genes 0.000 description 1
- 108091008578 transmembrane receptors Proteins 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- BPICBUSOMSTKRF-UHFFFAOYSA-N xylazine Chemical compound CC1=CC=CC(C)=C1NC1=NCCCS1 BPICBUSOMSTKRF-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Evolutionary Biology (AREA)
- Theoretical Computer Science (AREA)
- Medical Informatics (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51357403P | 2003-10-24 | 2003-10-24 | |
| EP04002722 | 2004-02-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA200604032B true ZA200604032B (en) | 2008-05-28 |
Family
ID=34524793
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA200604032A ZA200604032B (en) | 2003-10-24 | 2006-05-19 | High efficiency gene transfer and expression in mammaliar cells by a multiple transfection procedure of MAF sequences |
Country Status (16)
| Country | Link |
|---|---|
| US (3) | US8252917B2 (sk) |
| EP (5) | EP1675952A2 (sk) |
| JP (1) | JP5396653B2 (sk) |
| KR (4) | KR101229989B1 (sk) |
| CN (1) | CN1863913B (sk) |
| AT (1) | ATE551420T1 (sk) |
| AU (4) | AU2004284220B2 (sk) |
| CA (1) | CA2535836C (sk) |
| HK (4) | HK1151825A1 (sk) |
| IL (2) | IL175142A0 (sk) |
| IN (2) | IN2014DN02484A (sk) |
| PL (4) | PL1959011T3 (sk) |
| RU (1) | RU2375450C2 (sk) |
| SG (1) | SG147468A1 (sk) |
| WO (1) | WO2005040377A2 (sk) |
| ZA (1) | ZA200604032B (sk) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60312039T2 (de) | 2002-12-20 | 2007-11-08 | Chromagenics B.V. | Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können |
| PL1959011T3 (pl) | 2003-10-24 | 2012-07-31 | Selexis Sa | Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do macierzy |
| DE102004043207C5 (de) * | 2004-09-03 | 2010-09-09 | Südzucker AG Mannheim/Ochsenfurt | Wurzel- und xylemparenchymspezifischer Promotor |
| BRPI0517380A (pt) | 2004-11-08 | 2008-10-07 | Chromagenics Bv | molécula de dna, cassete de expressão, célula hospedeira, e, métodos para gerar uma célula hospedeira que expressa um polipeptìdeo de interesse e para produzir um polipeptìdeo de interesse |
| US8999667B2 (en) | 2004-11-08 | 2015-04-07 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| US8039230B2 (en) | 2004-11-08 | 2011-10-18 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| US20060195935A1 (en) | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| US7968700B2 (en) | 2006-03-20 | 2011-06-28 | Chromagenics B.V. | Expression augmenting DNA fragments, use thereof, and methods for finding thereof |
| SG176501A1 (en) * | 2006-08-23 | 2011-12-29 | Selexis Sa | Matrix attachment regions (mars) for increasing transcription and uses thereof |
| EP1975228A1 (en) | 2007-03-28 | 2008-10-01 | Fachhochschule Mannheim | Polynucleotides for enhancing expression of a polynucleotide of interest |
| PL2222710T3 (pl) | 2007-12-24 | 2017-01-31 | Id Biomedical Corporation Of Quebec | Rekombinowane antygeny rsv |
| CN101580842B (zh) * | 2008-05-15 | 2011-02-09 | 中国农业科学院生物技术研究所 | 一种增强基因表达的dna分子及其应用 |
| WO2010081890A1 (en) | 2009-01-19 | 2010-07-22 | Innate Pharma | Anti-kir3d antibodies |
| BRPI1015917A2 (pt) | 2009-06-24 | 2019-08-27 | Glaxosmithkline Biologicals Sa | antígenos de rsv recombinantes. |
| US8889146B2 (en) | 2009-06-24 | 2014-11-18 | Glaxosmithkline Biologicals, Sa | Vaccine |
| HUE058971T2 (hu) | 2009-07-15 | 2022-09-28 | Glaxosmithkline Biologicals Sa | RSV F fehérjekészítmények és eljárások azok elõállítására |
| CA2774470A1 (en) * | 2009-09-18 | 2011-03-24 | Selexis S.A. | Products and methods for enhanced transgene expression and processing |
| WO2011070405A1 (en) * | 2009-12-07 | 2011-06-16 | Avesthagen Limited | An expression vector carrying scaffold/matrix attachment region(s) |
| WO2012019630A1 (en) | 2010-08-13 | 2012-02-16 | Curevac Gmbh | Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded protein |
| WO2012095514A1 (en) | 2011-01-14 | 2012-07-19 | Vivalis | Recombinant protein production system |
| EP2697375B1 (en) | 2011-04-13 | 2017-10-18 | National Research Council of Canada | Expression system with scaffold attachment region (sar) element from interferon 2 |
| WO2013120498A1 (en) | 2012-02-15 | 2013-08-22 | Curevac Gmbh | Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded allergenic antigen or an autoimmune self-antigen |
| WO2013120500A1 (en) | 2012-02-15 | 2013-08-22 | Curevac Gmbh | Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded tumour antigen |
| WO2013120497A1 (en) * | 2012-02-15 | 2013-08-22 | Curevac Gmbh | Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded therapeutic protein |
| WO2013120499A1 (en) * | 2012-02-15 | 2013-08-22 | Curevac Gmbh | Nucleic acid comprising or coding for a histone stem-loop and a poly (a) sequence or a polyadenylation signal for increasing the expression of an encoded pathogenic antigen |
| US9683233B2 (en) | 2012-03-27 | 2017-06-20 | Curevac Ag | Artificial nucleic acid molecules for improved protein or peptide expression |
| PT3536797T (pt) | 2013-02-01 | 2023-03-15 | Selexis Sa | Expressão e processamento reforçado do transgene |
| CN103667440A (zh) * | 2013-09-05 | 2014-03-26 | 谢小冬 | Gata4基因snp位点的用途 |
| WO2015060935A1 (en) | 2013-10-22 | 2015-04-30 | Lubris, Llc | Control of rheological properties of mixed hyaluronate/lubricin solutions |
| CN109576269B (zh) * | 2014-04-01 | 2020-08-28 | 三生国健药业（上海）股份有限公司 | 一种含mar核心片段的动物细胞表达载体 |
| CN104974933B (zh) * | 2014-04-04 | 2017-08-15 | 上海泰因生物技术有限公司 | 一种大规模连续多次悬浮瞬转表达重组蛋白的装置和方法 |
| US11518791B2 (en) | 2016-05-23 | 2022-12-06 | Luxembourg Institute Of Health (Lih) | Multifunctional heteromultimeric constructs |
| JP2022509451A (ja) | 2018-10-24 | 2022-01-20 | セレキシス エスエー | 発現系、組み換え細胞及びその使用 |
| US20220017916A1 (en) * | 2018-12-07 | 2022-01-20 | Celltheon Corporation | Matrix attachment regions and uses in promoting gene expression |
| CN115175925A (zh) | 2020-02-24 | 2022-10-11 | 诺华股份有限公司 | 重组产生的多肽的纯化 |
| AU2020447192A1 (en) * | 2020-05-11 | 2023-01-19 | Samsung Bioepis Co., Ltd. | Human genome-derived polynucleotide and method for producing polypeptide of interest using same |
Family Cites Families (92)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1077994A (en) * | 1963-04-18 | 1967-08-02 | Kobe Steel Ltd | Process for producing cold-forged products from tempered steel wire |
| DE2605560C3 (de) | 1976-02-12 | 1979-08-30 | Siemens Ag, 1000 Berlin Und 8000 Muenchen | Aufschlußverfahren für Biomaterialien |
| DE3376405D1 (en) | 1982-12-23 | 1988-06-01 | Britax Weathershields | Vehicle sliding roof |
| JP2874751B2 (ja) | 1986-04-09 | 1999-03-24 | ジェンザイム・コーポレーション | 希望する蛋白質をミルク中へ分泌する遺伝子移植動物 |
| US4873316A (en) | 1987-06-23 | 1989-10-10 | Biogen, Inc. | Isolation of exogenous recombinant proteins from the milk of transgenic mammals |
| GB8925590D0 (en) | 1989-11-13 | 1990-01-04 | Central Blood Lab Authority | Monoclonal antibodies |
| US6063630A (en) | 1991-11-05 | 2000-05-16 | Transkaryotic Therapies, Inc. | Targeted introduction of DNA into primary or secondary cells and their use for gene therapy |
| US5733761A (en) | 1991-11-05 | 1998-03-31 | Transkaryotic Therapies, Inc. | Protein production and protein delivery |
| DK0663921T3 (da) | 1992-10-05 | 2002-09-02 | Univ North Carolina State | Fremgangsmåde til forøgelse af ekspression og reduktion af ekspressionsvariabilitet af fremmede gener i planteceller |
| US5610053A (en) | 1993-04-07 | 1997-03-11 | The United States Of America As Represented By The Department Of Health And Human Services | DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells |
| US5464758A (en) | 1993-06-14 | 1995-11-07 | Gossen; Manfred | Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters |
| US5888981A (en) | 1993-06-14 | 1999-03-30 | Basf Aktiengesellschaft | Methods for regulating gene expression |
| US5907078A (en) | 1994-12-09 | 1999-05-25 | Greenberg; Norman M. | Transgenic mouse model for prostate cancer |
| US5705364A (en) | 1995-06-06 | 1998-01-06 | Genentech, Inc. | Mammalian cell culture process |
| DE19541450C2 (de) * | 1995-11-07 | 1997-10-02 | Gsf Forschungszentrum Umwelt | Genkonstrukt und dessen Verwendung |
| US6596514B2 (en) | 1996-01-11 | 2003-07-22 | Immunex Corporation | Expression augmenting sequence elements (EASE) for eukaryotic expression systems |
| US5773695A (en) | 1996-01-26 | 1998-06-30 | North Carolina State University | Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells |
| AU2064297A (en) | 1996-02-29 | 1997-09-16 | Immusol, Inc | Hepatitis c virus ribozymes |
| CA2256484A1 (en) | 1996-06-06 | 1997-12-11 | Novartis Ag | Vectors comprising sar elements |
| EP1471144B1 (en) | 1996-12-02 | 2010-06-23 | Genaera Corporation | Biological variability of asthma associated factor AAF2 (IL-9 receptor) useful in treating and diagnosing atopic allergies including asthma and related disorders |
| US6245974B1 (en) * | 1997-08-06 | 2001-06-12 | North Carolina State University | Matrix attachment regions |
| US6897066B1 (en) | 1997-09-26 | 2005-05-24 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
| AU734476B2 (en) | 1997-11-05 | 2001-06-14 | Baylor College Of Medicine | Sequences for targeting metastatic cells |
| US6635806B1 (en) | 1998-05-14 | 2003-10-21 | Dekalb Genetics Corporation | Methods and compositions for expression of transgenes in plants |
| US6004284A (en) | 1998-06-04 | 1999-12-21 | Alcon Laboratories, Inc. | Surgical handpiece |
| KR100795626B1 (ko) | 1998-07-21 | 2008-01-17 | 코브라 바이오매뉴팩쳐링 피엘씨. | 도처에 있는 염색질 개방 요소(유씨오이)를 포함하는폴리뉴클레오티드 |
| US6338066B1 (en) * | 1998-09-25 | 2002-01-08 | International Business Machines Corporation | Surfaid predictor: web-based system for predicting surfer behavior |
| DE69904744T2 (de) | 1998-10-07 | 2003-10-23 | Adobe Systems, Inc. | Zuteilung des zugriffs auf einen datensatz |
| DE19848017A1 (de) | 1998-10-17 | 2000-04-20 | Multigene Biotech Gmbh | Episomal replizierender Vektor zur Expression eines Transgens in Säugerzellen |
| WO2000032800A1 (en) | 1998-12-01 | 2000-06-08 | Dow Agrosciences Llc | Artificial matrix attachment region for increasing expression of genes introduced in plant cells |
| US6225113B1 (en) | 1998-12-04 | 2001-05-01 | Genvec, Inc. | Use of trans-activation and cis-activation to modulate the persistence of expression of a transgene in an at least E4-deficient adenovirus |
| US6232526B1 (en) | 1999-05-14 | 2001-05-15 | Dekalb Genetics Corp. | Maize A3 promoter and methods for use thereof |
| US6194636B1 (en) | 1999-05-14 | 2001-02-27 | Dekalb Genetics Corp. | Maize RS324 promoter and methods for use thereof |
| US6429357B1 (en) | 1999-05-14 | 2002-08-06 | Dekalb Genetics Corp. | Rice actin 2 promoter and intron and methods for use thereof |
| US6207879B1 (en) | 1999-05-14 | 2001-03-27 | Dekalb Genetics Corporation | Maize RS81 promoter and methods for use thereof |
| AU4940700A (en) | 1999-05-28 | 2000-12-18 | Sangamo Biosciences, Inc. | Gene switches |
| US20050129669A1 (en) | 1999-06-21 | 2005-06-16 | Transkaryotic Therapies, Inc., A Massachusetts Corporation | DNA construct for effecting homologous recombination and uses thereof |
| US6972349B1 (en) | 1999-11-12 | 2005-12-06 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
| JP3507858B2 (ja) | 1999-11-19 | 2004-03-15 | 奈良先端科学技術大学院大学長 | 植物において外来遺伝子の発現を安定化させるｄｎａ断片、外来遺伝子を植物において安定に発現させる遺伝子導入方法、外来遺伝子を安定に発現させた形質転換植物 |
| AU7521500A (en) | 1999-11-30 | 2001-06-12 | Novartis Ag | Increased transgene expression in retroviral vectors having scaffold attachment region |
| US6864085B2 (en) | 1999-12-14 | 2005-03-08 | Novartis Ag | Bovine immunodeficiency virus (BIV) based vectors |
| US20020127199A1 (en) | 2000-02-03 | 2002-09-12 | Tang Y. Tom | Novel nucleic acids and polypeptides |
| US6821775B1 (en) | 2000-02-11 | 2004-11-23 | Genvec, Inc. | Viral vector encoding pigment epithelium-derived factor |
| DE10006886A1 (de) | 2000-02-16 | 2001-08-23 | Hepavec Ag Fuer Gentherapie | Nichthumaner helferabhängiger Virusvektor |
| US6569681B1 (en) | 2000-03-14 | 2003-05-27 | Transkaryotic Therapies, Inc. | Methods of improving homologous recombination |
| US6747189B1 (en) | 2000-03-21 | 2004-06-08 | Dekalb Genetics Corporation | Maize glycine rich protein promoter compositions and methods for use thereof |
| US6730826B2 (en) | 2000-03-22 | 2004-05-04 | University Of Kentucky Research Foundation | Plant trichome gland specific promoter sequence |
| CA2409408A1 (en) | 2000-06-26 | 2002-01-03 | Ulrich Laemmli | Modulation of chromosome function by chromatin remodeling agents |
| US20030157715A1 (en) | 2000-06-26 | 2003-08-21 | Ulrich Laemmli | Modulation of chromosome function by chromatin remodeling agents |
| JP2004518405A (ja) | 2000-07-27 | 2004-06-24 | アポゲーネ・ゲーエムベーハー・ウント・コー・カーゲー | 組換えタンパク質、細胞、および生物を産生するための体細胞クローニング遺伝子移入 |
| KR100408844B1 (ko) | 2000-07-29 | 2003-12-06 | 한국산업기술평가원 | 동물세포 발현벡터 |
| JP2004507250A (ja) | 2000-08-30 | 2004-03-11 | ノース・キャロライナ・ステイト・ユニヴァーシティ | タンパク質含量を変化させる分子デコイを含有するトランスジェニック植物 |
| WO2002020811A2 (en) | 2000-09-09 | 2002-03-14 | Basf Plant Science Gmbh | Modified tet-inducible system for regulation of gene expression in plants |
| GB0022995D0 (en) | 2000-09-20 | 2000-11-01 | Cobra Therapeutics Ltd | Polynucleotide |
| US6919204B2 (en) | 2000-09-29 | 2005-07-19 | Sangamo Biosciences, Inc. | Modulation of gene expression using localization domains |
| WO2002046208A2 (en) | 2000-11-01 | 2002-06-13 | Elusys Therapeutics, Inc. | Method of producing biospecific molecules by protein trans-splicing |
| DE10061150A1 (de) | 2000-12-08 | 2002-06-13 | Icon Genetics Ag | Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen |
| US7259010B2 (en) | 2000-12-15 | 2007-08-21 | Pangen Biotech Inc. | Expression vector for animal cell containing nuclear matrix attachment region of interferon beta |
| US7151204B2 (en) | 2001-01-09 | 2006-12-19 | Monsanto Technology Llc | Maize chloroplast aldolase promoter compositions and methods for use thereof |
| CA2435972C (en) | 2001-01-26 | 2011-09-13 | University Of Lausanne | Matrix attachment regions and methods for use thereof |
| DE10109780A1 (de) | 2001-02-28 | 2002-09-05 | Cardion Ag | Episomale Vektoren enthaltend Matrix-Anheftungsregionen zur zellspezifischen Genexpression |
| EP1365801A4 (en) | 2001-03-08 | 2004-10-13 | Nuvelo Inc | METHOD AND MATERIALS IN CONNECTION WITH FIBULIN-LIKE POLYPEPTIDES AND POLYNUCLEOTIDES |
| JP4817514B2 (ja) | 2001-03-09 | 2011-11-16 | 協和発酵キリン株式会社 | 新規動物細胞用ベクターおよびその使用 |
| AU2002252370A1 (en) | 2001-03-12 | 2002-09-24 | Irm, Llc. | Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters |
| US7070993B2 (en) | 2001-03-13 | 2006-07-04 | Novartis Ag | Lentiviral packaging constructs |
| EP1478751A4 (en) * | 2001-03-30 | 2005-10-19 | Avigenics Inc | LYSOZYM PROMOTER IN BIRDS |
| DE10129010A1 (de) | 2001-06-13 | 2002-12-19 | Icon Genetics Ag | Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen |
| US20030032597A1 (en) | 2001-07-31 | 2003-02-13 | Sebestyen Magdolna G. | Targeting nucleic acids to a cellular nucleus |
| KR100464962B1 (ko) * | 2001-09-14 | 2005-01-05 | 삼화강봉주식회사 | 냉간압조 특성이 우수한 조질 강선 |
| US7122715B2 (en) | 2001-09-20 | 2006-10-17 | Monsanto Technology Llc | In vitro method to create circular molecules for use in transformation |
| AU2002341795A1 (en) | 2001-09-21 | 2003-04-01 | Avigenics, Inc. | Production of transgenic avians using sperm-mediated transfection |
| US7138278B2 (en) | 2001-11-20 | 2006-11-21 | Monsanto Technology, L.L.C. | Maize cytoplasmic glutamine synthetase promoter compositions and methods for use thereof |
| FR2832423B1 (fr) | 2001-11-22 | 2004-10-08 | Vivalis | Systeme d'expression de proteines exogenes dans un systeme aviaire |
| US6875588B2 (en) | 2001-11-30 | 2005-04-05 | Avigenics, Inc. | Ovomucoid promoter and methods of use |
| CA2427190A1 (en) | 2002-04-30 | 2003-10-30 | Alberta Research Council Inc. | Production of recombinant epidermal growth factor in plants |
| US20030224477A1 (en) | 2002-05-31 | 2003-12-04 | Heartlein Michael W. | Optimized promoter constructs |
| WO2003106658A2 (en) | 2002-06-18 | 2003-12-24 | Zymogenetics, Inc. | Hybrid vector having a cytomegalovirus enhancer and myeloproliferative sarcoma virus promoter |
| US20040016015A1 (en) | 2002-07-17 | 2004-01-22 | Nguyen Thanh-Tuyen T. | High efficiency germline transformation system |
| US6933136B2 (en) | 2002-09-20 | 2005-08-23 | Novo Nordisk A/S | Method for making recombinant proteins |
| CN1263860C (zh) | 2002-09-30 | 2006-07-12 | 华南农业大学 | 多基因载体的构建方法与应用 |
| AU2003302777A1 (en) | 2002-12-05 | 2004-06-30 | Regulome Corporation | Profiled regulatory sites useful for gene control |
| WO2004053137A2 (en) | 2002-12-11 | 2004-06-24 | Cytos Biotechnology Ag | Method for protein production |
| AU2002953381A0 (en) | 2002-12-18 | 2003-01-09 | Diatech Pty Ltd | In vivo affinity maturation scheme |
| EP1445320A1 (en) | 2003-02-05 | 2004-08-11 | ARTEMIS Pharmaceuticals GmbH | Automated gene-targeting using non-toxic detectable markers |
| US7585963B2 (en) | 2003-04-15 | 2009-09-08 | Synageva Biopharma Corp. | Chicken ovalbumin nucleotide sequence |
| WO2004106375A1 (en) | 2003-05-30 | 2004-12-09 | Merus Biopharmaceuticals B.V. I.O. | Fab library for the preparation of anti vegf and anti rabies virus fabs |
| EP1644508A1 (en) * | 2003-07-11 | 2006-04-12 | Cytos Biotechnology AG | Gene expression system |
| WO2005021765A2 (en) | 2003-08-27 | 2005-03-10 | Orf Liftaekni Hf. | Enhancing accumulation of heterologous polypeptides in plant seeds through targeted suppression of endogenous storage proteins |
| PL1675956T3 (pl) | 2003-10-21 | 2011-06-30 | Merck Serono Sa | Minimalna sekwencja DNA działająca jako insulator chromatyny i jej zastosowanie do wyrażania białek |
| PL1959011T3 (pl) * | 2003-10-24 | 2012-07-31 | Selexis Sa | Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do macierzy |
| SG176501A1 (en) | 2006-08-23 | 2011-12-29 | Selexis Sa | Matrix attachment regions (mars) for increasing transcription and uses thereof |
| WO2008073303A2 (en) * | 2006-12-07 | 2008-06-19 | Switchgear Genomics | Transcriptional regulatory elements of biological pathways, tools, and methods |
-
2004
- 2004-10-22 PL PL08153753T patent/PL1959011T3/pl unknown
- 2004-10-22 US US10/595,495 patent/US8252917B2/en active Active
- 2004-10-22 PL PL10178873T patent/PL2298898T3/pl unknown
- 2004-10-22 PL PL10178871T patent/PL2292754T3/pl unknown
- 2004-10-22 WO PCT/EP2004/011974 patent/WO2005040377A2/en active Application Filing
- 2004-10-22 EP EP04790766A patent/EP1675952A2/en not_active Withdrawn
- 2004-10-22 KR KR1020117023132A patent/KR101229989B1/ko active IP Right Grant
- 2004-10-22 CN CN2004800292606A patent/CN1863913B/zh active Active
- 2004-10-22 EP EP08153753A patent/EP1959011B1/en active Active
- 2004-10-22 SG SG200807906-3A patent/SG147468A1/en unknown
- 2004-10-22 RU RU2006117757/13A patent/RU2375450C2/ru active
- 2004-10-22 AU AU2004284220A patent/AU2004284220B2/en not_active Ceased
- 2004-10-22 IN IN2484DEN2014 patent/IN2014DN02484A/en unknown
- 2004-10-22 KR KR1020067006395A patent/KR101222025B1/ko active IP Right Grant
- 2004-10-22 JP JP2006536060A patent/JP5396653B2/ja active Active
- 2004-10-22 IN IN2483DEN2014 patent/IN2014DN02483A/en unknown
- 2004-10-22 KR KR1020117023134A patent/KR101269656B1/ko active IP Right Grant
- 2004-10-22 EP EP10178873A patent/EP2298898B1/en active Active
- 2004-10-22 PL PL10178866T patent/PL2287302T3/pl unknown
- 2004-10-22 KR KR1020117023131A patent/KR101230838B1/ko active IP Right Grant
- 2004-10-22 CA CA2535836A patent/CA2535836C/en active Active
- 2004-10-22 AT AT08153753T patent/ATE551420T1/de active
- 2004-10-22 EP EP10178871A patent/EP2292754B1/en active Active
- 2004-10-22 EP EP10178866A patent/EP2287302B1/en active Active
-
2006
- 2006-04-24 IL IL175142A patent/IL175142A0/en unknown
- 2006-05-19 ZA ZA200604032A patent/ZA200604032B/en unknown
-
2008
- 2008-11-06 HK HK11105802.4A patent/HK1151825A1/xx unknown
- 2008-11-06 HK HK11103767.2A patent/HK1149777A1/xx not_active IP Right Cessation
- 2008-11-06 HK HK11105801.5A patent/HK1151824A1/xx not_active IP Right Cessation
- 2008-11-06 HK HK08112192.3A patent/HK1120549A1/xx not_active IP Right Cessation
-
2009
- 2009-05-21 IL IL198863A patent/IL198863A/en active IP Right Grant
-
2010
- 2010-10-27 AU AU2010236072A patent/AU2010236072B2/en not_active Ceased
- 2010-10-27 AU AU2010236093A patent/AU2010236093B2/en not_active Ceased
- 2010-10-27 AU AU2010236091A patent/AU2010236091B2/en active Active
-
2012
- 2012-06-28 US US13/536,383 patent/US9879297B2/en active Active
-
2017
- 2017-12-19 US US15/846,279 patent/US10669562B2/en active Active
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10669562B2 (en) | High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences | |
| CA2884687C (en) | High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of mar sequences |