ZA200601177B - Methods for producing recombinant proteins - Google Patents
Methods for producing recombinant proteins Download PDFInfo
- Publication number
- ZA200601177B ZA200601177B ZA200601177A ZA200601177A ZA200601177B ZA 200601177 B ZA200601177 B ZA 200601177B ZA 200601177 A ZA200601177 A ZA 200601177A ZA 200601177 A ZA200601177 A ZA 200601177A ZA 200601177 B ZA200601177 B ZA 200601177B
- Authority
- ZA
- South Africa
- Prior art keywords
- growth rate
- recombinant protein
- supernatant
- periplasm
- protein
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title claims description 89
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title claims description 89
- 238000000034 method Methods 0.000 title claims description 79
- 230000012010 growth Effects 0.000 claims description 148
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 84
- 210000004027 cell Anatomy 0.000 claims description 71
- 241000588724 Escherichia coli Species 0.000 claims description 66
- 210000001322 periplasm Anatomy 0.000 claims description 51
- 239000006228 supernatant Substances 0.000 claims description 51
- 230000014509 gene expression Effects 0.000 claims description 38
- 238000011084 recovery Methods 0.000 claims description 28
- 229910019142 PO4 Inorganic materials 0.000 claims description 23
- 238000000638 solvent extraction Methods 0.000 claims description 22
- 239000010452 phosphate Substances 0.000 claims description 18
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 17
- 230000001939 inductive effect Effects 0.000 claims description 16
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 15
- 229910052799 carbon Inorganic materials 0.000 claims description 15
- 238000004113 cell culture Methods 0.000 claims description 14
- 239000012228 culture supernatant Substances 0.000 claims description 10
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 9
- 239000008101 lactose Substances 0.000 claims description 8
- 229910052760 oxygen Inorganic materials 0.000 claims description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 239000001301 oxygen Substances 0.000 claims description 7
- 230000002829 reductive effect Effects 0.000 claims description 7
- 239000012634 fragment Substances 0.000 claims description 4
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 3
- 229930091371 Fructose Natural products 0.000 claims description 2
- 239000005715 Fructose Substances 0.000 claims description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 description 84
- 102000004169 proteins and genes Human genes 0.000 description 78
- 238000000855 fermentation Methods 0.000 description 40
- 230000004151 fermentation Effects 0.000 description 40
- 230000006698 induction Effects 0.000 description 37
- 235000015097 nutrients Nutrition 0.000 description 20
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- 239000002028 Biomass Substances 0.000 description 15
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- 230000001965 increasing effect Effects 0.000 description 11
- 239000000411 inducer Substances 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 108090000765 processed proteins & peptides Proteins 0.000 description 9
- 238000000605 extraction Methods 0.000 description 8
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- 229920001184 polypeptide Polymers 0.000 description 8
- 102000004196 processed proteins & peptides Human genes 0.000 description 8
- 239000000284 extract Substances 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- 239000002054 inoculum Substances 0.000 description 6
- 238000002965 ELISA Methods 0.000 description 5
- 230000001276 controlling effect Effects 0.000 description 5
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- 239000013612 plasmid Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 108010076504 Protein Sorting Signals Proteins 0.000 description 4
- 239000004098 Tetracycline Substances 0.000 description 4
- 238000013019 agitation Methods 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 238000011143 downstream manufacturing Methods 0.000 description 4
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000012552 review Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 229960002180 tetracycline Drugs 0.000 description 4
- 229930101283 tetracycline Natural products 0.000 description 4
- 235000019364 tetracycline Nutrition 0.000 description 4
- 150000003522 tetracyclines Chemical class 0.000 description 4
- 241001302584 Escherichia coli str. K-12 substr. W3110 Species 0.000 description 3
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- 101000578492 Escherichia coli Lysis protein Proteins 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 2
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- 108010079246 OMPA outer membrane proteins Proteins 0.000 description 2
- 108010090127 Periplasmic Proteins Proteins 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
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- 210000000805 cytoplasm Anatomy 0.000 description 2
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- 238000011026 diafiltration Methods 0.000 description 2
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- 230000001900 immune effect Effects 0.000 description 2
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- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
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- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000012846 protein folding Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000012134 supernatant fraction Substances 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 101100136076 Aspergillus oryzae (strain ATCC 42149 / RIB 40) pel1 gene Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
- 102000017727 Immunoglobulin Variable Region Human genes 0.000 description 1
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- SRBFZHDQGSBBOR-HWQSCIPKSA-N L-arabinopyranose Chemical compound O[C@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-HWQSCIPKSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 235000019687 Lamb Nutrition 0.000 description 1
- 229930192392 Mitomycin Natural products 0.000 description 1
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108700006385 OmpF Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 101100084022 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) lapA gene Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
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- 239000002299 complementary DNA Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
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- 230000002950 deficient Effects 0.000 description 1
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- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 101150036274 kil gene Proteins 0.000 description 1
- 150000002597 lactoses Chemical class 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229960004857 mitomycin Drugs 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 101150040383 pel2 gene Proteins 0.000 description 1
- 101150050446 pelB gene Proteins 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000008823 permeabilization Effects 0.000 description 1
- 101150009573 phoA gene Proteins 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000004007 reversed phase HPLC Methods 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Jellies, Jams, And Syrups (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0321100.0A GB0321100D0 (en) | 2003-09-09 | 2003-09-09 | Biological products |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA200601177B true ZA200601177B (en) | 2007-04-25 |
Family
ID=29226759
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA200601177A ZA200601177B (en) | 2003-09-09 | 2004-09-02 | Methods for producing recombinant proteins |
Country Status (20)
| Country | Link |
|---|---|
| US (2) | US7754447B2 (no) |
| EP (1) | EP1664278B1 (no) |
| JP (1) | JP4568722B2 (no) |
| KR (1) | KR20060125704A (no) |
| CN (1) | CN1849389B (no) |
| AT (1) | ATE454444T1 (no) |
| AU (1) | AU2004270929B2 (no) |
| BR (1) | BRPI0413813A (no) |
| CA (1) | CA2536099C (no) |
| DE (1) | DE602004025011D1 (no) |
| EA (1) | EA200600554A1 (no) |
| ES (1) | ES2339352T3 (no) |
| GB (1) | GB0321100D0 (no) |
| IL (1) | IL173474A0 (no) |
| MX (1) | MXPA06002589A (no) |
| NO (1) | NO20061576L (no) |
| PL (1) | PL1664278T3 (no) |
| SG (1) | SG145696A1 (no) |
| WO (1) | WO2005024000A1 (no) |
| ZA (1) | ZA200601177B (no) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2608579C (en) * | 2005-05-26 | 2019-09-10 | Cytos Biotechnology Ag | Scalable fermentation process |
| KR102071834B1 (ko) | 2009-10-26 | 2020-01-30 | 에프. 호프만-라 로슈 아게 | 글리코실화된 면역글로불린의 제조 방법 |
| EP2817625A2 (en) | 2012-02-23 | 2014-12-31 | Stage Cell Therapeutics GmbH | Chromatographic isolation of cells and other complex biological materials |
| DK2825633T3 (da) * | 2012-03-12 | 2019-05-20 | Hanmi Science Co Ltd | Fremgangsmåde til dyrkning af E. coli-celler til høj densitet |
| AU2016229982B2 (en) | 2015-03-09 | 2020-06-18 | Intekrin Therapeutics, Inc. | Methods for the treatment of nonalcoholic fatty liver disease and/or lipodystrophy |
| MA45489A (fr) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | Procédés de culture de cellules, kits et appareil associés |
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| EP0335567A3 (en) | 1988-03-30 | 1990-11-14 | Biogen, Inc. | Controlled release of periplasmic proteins into the medium |
| US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| GB8928874D0 (en) | 1989-12-21 | 1990-02-28 | Celltech Ltd | Humanised antibodies |
| CA2090126C (en) | 1990-08-02 | 2002-10-22 | John W. Schrader | Methods for the production of proteins with a desired function |
| GB9215540D0 (en) | 1992-07-22 | 1992-09-02 | Celltech Ltd | Protein expression system |
| AU3944002A (en) | 2000-11-03 | 2002-05-27 | Genentech Inc | Metabolic rate shifts in fermentations expressing recombinant proteins |
| US7785830B2 (en) | 2003-08-13 | 2010-08-31 | Sandoz Ag | Expression vectors, transformed host cells and fermentation process for the production of recombinant polypeptides |
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- 2004-09-02 SG SG200805643-4A patent/SG145696A1/en unknown
- 2004-09-02 JP JP2006525876A patent/JP4568722B2/ja not_active Expired - Fee Related
- 2004-09-02 KR KR1020067004872A patent/KR20060125704A/ko not_active Application Discontinuation
- 2004-09-02 US US10/571,367 patent/US7754447B2/en not_active Ceased
- 2004-09-02 PL PL04768294T patent/PL1664278T3/pl unknown
- 2004-09-02 AU AU2004270929A patent/AU2004270929B2/en not_active Ceased
- 2004-09-02 AT AT04768294T patent/ATE454444T1/de not_active IP Right Cessation
- 2004-09-02 BR BRPI0413813-9A patent/BRPI0413813A/pt not_active IP Right Cessation
- 2004-09-02 US US13/547,892 patent/USRE44512E1/en active Active
- 2004-09-02 ZA ZA200601177A patent/ZA200601177B/en unknown
- 2004-09-02 EP EP04768294A patent/EP1664278B1/en not_active Expired - Lifetime
- 2004-09-02 EA EA200600554A patent/EA200600554A1/ru unknown
- 2004-09-02 WO PCT/GB2004/003747 patent/WO2005024000A1/en active Application Filing
- 2004-09-02 MX MXPA06002589A patent/MXPA06002589A/es unknown
- 2004-09-02 ES ES04768294T patent/ES2339352T3/es not_active Expired - Lifetime
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- 2004-09-02 DE DE602004025011T patent/DE602004025011D1/de not_active Expired - Lifetime
- 2004-09-02 CN CN2004800258836A patent/CN1849389B/zh not_active Expired - Fee Related
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2006
- 2006-01-31 IL IL173474A patent/IL173474A0/en unknown
- 2006-04-07 NO NO20061576A patent/NO20061576L/no not_active Application Discontinuation
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|---|---|
| EA200600554A1 (ru) | 2006-08-25 |
| AU2004270929A1 (en) | 2005-03-17 |
| EP1664278B1 (en) | 2010-01-06 |
| GB0321100D0 (en) | 2003-10-08 |
| EP1664278A1 (en) | 2006-06-07 |
| BRPI0413813A (pt) | 2006-10-17 |
| CA2536099A1 (en) | 2005-03-17 |
| AU2004270929B2 (en) | 2010-01-21 |
| ATE454444T1 (de) | 2010-01-15 |
| CA2536099C (en) | 2015-11-17 |
| NO20061576L (no) | 2006-04-07 |
| US7754447B2 (en) | 2010-07-13 |
| MXPA06002589A (es) | 2006-06-05 |
| JP2007504815A (ja) | 2007-03-08 |
| CN1849389B (zh) | 2012-05-23 |
| USRE44512E1 (en) | 2013-10-01 |
| PL1664278T3 (pl) | 2010-06-30 |
| IL173474A0 (en) | 2006-06-11 |
| KR20060125704A (ko) | 2006-12-06 |
| JP4568722B2 (ja) | 2010-10-27 |
| US20080044817A1 (en) | 2008-02-21 |
| WO2005024000A1 (en) | 2005-03-17 |
| CN1849389A (zh) | 2006-10-18 |
| DE602004025011D1 (de) | 2010-02-25 |
| ES2339352T3 (es) | 2010-05-19 |
| SG145696A1 (en) | 2008-09-29 |
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