EA200600554A1 - Способ получения рекомбинантных белков - Google Patents
Способ получения рекомбинантных белковInfo
- Publication number
- EA200600554A1 EA200600554A1 EA200600554A EA200600554A EA200600554A1 EA 200600554 A1 EA200600554 A1 EA 200600554A1 EA 200600554 A EA200600554 A EA 200600554A EA 200600554 A EA200600554 A EA 200600554A EA 200600554 A1 EA200600554 A1 EA 200600554A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- recombinant protein
- periplasm
- stage
- growth rate
- supernatant
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Jellies, Jams, And Syrups (AREA)
Abstract
Настоящее изобретение охватывает способ регулирования распределения рекомбинантного белка между супернатантом и периплазмой в культурах клеток-хозяев Е coli, отличающийся тем, что экспрессия рекомбинантного белка в указанных клетках осуществляется под контролем индуцибельной системы, причём указанный способ включает: а) получение культуры клеток Е. coli; б) изменение скорости роста клеток-хозяев Е. coli; в) индуцирование экспрессии рекомбинантного белка, при этом стадии (б) и (в) можно осуществлять в любом порядке или одновременно; и затем г) определение выхода рекомбинантного белка в культуральном супернатанте и в периплазме клетки-хозяина Е. coli; д) сравнение выхода, определённого на стадии (г), с выходом, определённым в том случае, когда на стадии (б) используют по меньшей мере одну иную скорость роста; е) выбор в результате сравнения, сделанного на стадии (д), такой скорости роста, при которой распределение рекомбинантного белка между супернатантом и периплазмой наиболее соответствует первичной регенерации рекомбинантного белка.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0321100.0A GB0321100D0 (en) | 2003-09-09 | 2003-09-09 | Biological products |
| PCT/GB2004/003747 WO2005024000A1 (en) | 2003-09-09 | 2004-09-02 | Methods for producing recombinant proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EA200600554A1 true EA200600554A1 (ru) | 2006-08-25 |
Family
ID=29226759
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA200600554A EA200600554A1 (ru) | 2003-09-09 | 2004-09-02 | Способ получения рекомбинантных белков |
Country Status (20)
| Country | Link |
|---|---|
| US (2) | US7754447B2 (ru) |
| EP (1) | EP1664278B1 (ru) |
| JP (1) | JP4568722B2 (ru) |
| KR (1) | KR20060125704A (ru) |
| CN (1) | CN1849389B (ru) |
| AT (1) | ATE454444T1 (ru) |
| AU (1) | AU2004270929B2 (ru) |
| BR (1) | BRPI0413813A (ru) |
| CA (1) | CA2536099C (ru) |
| DE (1) | DE602004025011D1 (ru) |
| EA (1) | EA200600554A1 (ru) |
| ES (1) | ES2339352T3 (ru) |
| GB (1) | GB0321100D0 (ru) |
| IL (1) | IL173474A0 (ru) |
| MX (1) | MXPA06002589A (ru) |
| NO (1) | NO20061576L (ru) |
| PL (1) | PL1664278T3 (ru) |
| SG (1) | SG145696A1 (ru) |
| WO (1) | WO2005024000A1 (ru) |
| ZA (1) | ZA200601177B (ru) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2608579C (en) * | 2005-05-26 | 2019-09-10 | Cytos Biotechnology Ag | Scalable fermentation process |
| KR102071834B1 (ko) | 2009-10-26 | 2020-01-30 | 에프. 호프만-라 로슈 아게 | 글리코실화된 면역글로불린의 제조 방법 |
| EP2817625A2 (en) | 2012-02-23 | 2014-12-31 | Stage Cell Therapeutics GmbH | Chromatographic isolation of cells and other complex biological materials |
| DK2825633T3 (da) * | 2012-03-12 | 2019-05-20 | Hanmi Science Co Ltd | Fremgangsmåde til dyrkning af E. coli-celler til høj densitet |
| AU2016229982B2 (en) | 2015-03-09 | 2020-06-18 | Intekrin Therapeutics, Inc. | Methods for the treatment of nonalcoholic fatty liver disease and/or lipodystrophy |
| MA45489A (fr) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | Procédés de culture de cellules, kits et appareil associés |
| US10941406B2 (en) | 2016-03-29 | 2021-03-09 | Geltor, Inc. | Expression of proteins in gram-negative bacteria wherein the ratio of periplasmic volume to cytoplasmic volume is between 0.5:1 and 10:1 |
| EP3606527A1 (en) | 2017-04-03 | 2020-02-12 | Coherus Biosciences, Inc. | Ppar-gamma agonist for treatment of progressive supranuclear palsy |
| TW201842335A (zh) | 2017-04-27 | 2018-12-01 | 德商朱諾醫療公司 | 寡聚粒子試劑及其使用方法 |
| US11180541B2 (en) | 2017-09-28 | 2021-11-23 | Geltor, Inc. | Recombinant collagen and elastin molecules and uses thereof |
| WO2020118043A1 (en) | 2018-12-07 | 2020-06-11 | Coherus Biosciences, Inc. | Methods for producing recombinant proteins |
| TW202104254A (zh) | 2019-04-12 | 2021-02-01 | 美商格爾托公司 | 重組彈性蛋白及其生產 |
| US20240092883A1 (en) | 2019-10-11 | 2024-03-21 | Coherus Biosciences, Inc. | Methods of purifying ranibizumab or a ranibizumab variant |
| WO2021072182A1 (en) * | 2019-10-11 | 2021-04-15 | Coherus Biosciences, Inc. | Methods for producing ranibizumab |
| CN113355264B (zh) * | 2021-06-28 | 2022-03-08 | 山东智汇达海洋生物科技有限公司 | 一株产甘油的嗜高温菌及其应用 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0335567A3 (en) | 1988-03-30 | 1990-11-14 | Biogen, Inc. | Controlled release of periplasmic proteins into the medium |
| US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| GB8928874D0 (en) | 1989-12-21 | 1990-02-28 | Celltech Ltd | Humanised antibodies |
| CA2090126C (en) | 1990-08-02 | 2002-10-22 | John W. Schrader | Methods for the production of proteins with a desired function |
| GB9215540D0 (en) | 1992-07-22 | 1992-09-02 | Celltech Ltd | Protein expression system |
| AU3944002A (en) | 2000-11-03 | 2002-05-27 | Genentech Inc | Metabolic rate shifts in fermentations expressing recombinant proteins |
| US7785830B2 (en) | 2003-08-13 | 2010-08-31 | Sandoz Ag | Expression vectors, transformed host cells and fermentation process for the production of recombinant polypeptides |
-
2003
- 2003-09-09 GB GBGB0321100.0A patent/GB0321100D0/en not_active Ceased
-
2004
- 2004-09-02 SG SG200805643-4A patent/SG145696A1/en unknown
- 2004-09-02 JP JP2006525876A patent/JP4568722B2/ja not_active Expired - Fee Related
- 2004-09-02 KR KR1020067004872A patent/KR20060125704A/ko not_active Application Discontinuation
- 2004-09-02 US US10/571,367 patent/US7754447B2/en not_active Ceased
- 2004-09-02 PL PL04768294T patent/PL1664278T3/pl unknown
- 2004-09-02 AU AU2004270929A patent/AU2004270929B2/en not_active Ceased
- 2004-09-02 AT AT04768294T patent/ATE454444T1/de not_active IP Right Cessation
- 2004-09-02 BR BRPI0413813-9A patent/BRPI0413813A/pt not_active IP Right Cessation
- 2004-09-02 US US13/547,892 patent/USRE44512E1/en active Active
- 2004-09-02 ZA ZA200601177A patent/ZA200601177B/en unknown
- 2004-09-02 EP EP04768294A patent/EP1664278B1/en not_active Expired - Lifetime
- 2004-09-02 EA EA200600554A patent/EA200600554A1/ru unknown
- 2004-09-02 WO PCT/GB2004/003747 patent/WO2005024000A1/en active Application Filing
- 2004-09-02 MX MXPA06002589A patent/MXPA06002589A/es unknown
- 2004-09-02 ES ES04768294T patent/ES2339352T3/es not_active Expired - Lifetime
- 2004-09-02 CA CA2536099A patent/CA2536099C/en not_active Expired - Lifetime
- 2004-09-02 DE DE602004025011T patent/DE602004025011D1/de not_active Expired - Lifetime
- 2004-09-02 CN CN2004800258836A patent/CN1849389B/zh not_active Expired - Fee Related
-
2006
- 2006-01-31 IL IL173474A patent/IL173474A0/en unknown
- 2006-04-07 NO NO20061576A patent/NO20061576L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| AU2004270929A1 (en) | 2005-03-17 |
| EP1664278B1 (en) | 2010-01-06 |
| GB0321100D0 (en) | 2003-10-08 |
| EP1664278A1 (en) | 2006-06-07 |
| BRPI0413813A (pt) | 2006-10-17 |
| CA2536099A1 (en) | 2005-03-17 |
| AU2004270929B2 (en) | 2010-01-21 |
| ATE454444T1 (de) | 2010-01-15 |
| CA2536099C (en) | 2015-11-17 |
| NO20061576L (no) | 2006-04-07 |
| US7754447B2 (en) | 2010-07-13 |
| ZA200601177B (en) | 2007-04-25 |
| MXPA06002589A (es) | 2006-06-05 |
| JP2007504815A (ja) | 2007-03-08 |
| CN1849389B (zh) | 2012-05-23 |
| USRE44512E1 (en) | 2013-10-01 |
| PL1664278T3 (pl) | 2010-06-30 |
| IL173474A0 (en) | 2006-06-11 |
| KR20060125704A (ko) | 2006-12-06 |
| JP4568722B2 (ja) | 2010-10-27 |
| US20080044817A1 (en) | 2008-02-21 |
| WO2005024000A1 (en) | 2005-03-17 |
| CN1849389A (zh) | 2006-10-18 |
| DE602004025011D1 (de) | 2010-02-25 |
| ES2339352T3 (es) | 2010-05-19 |
| SG145696A1 (en) | 2008-09-29 |
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