ZA200600017B - Insecticidal proteins secreted from Bacillus thuringiensis and uses therefor - Google Patents
Insecticidal proteins secreted from Bacillus thuringiensis and uses therefor Download PDFInfo
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- ZA200600017B ZA200600017B ZA200600017A ZA200600017A ZA200600017B ZA 200600017 B ZA200600017 B ZA 200600017B ZA 200600017 A ZA200600017 A ZA 200600017A ZA 200600017 A ZA200600017 A ZA 200600017A ZA 200600017 B ZA200600017 B ZA 200600017B
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- C—CHEMISTRY; METALLURGY
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- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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EC (1) | ECSP066270A (zh) |
MX (1) | MXPA06000293A (zh) |
RU (1) | RU2382822C2 (zh) |
WO (1) | WO2005019414A2 (zh) |
ZA (1) | ZA200600017B (zh) |
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MXPA06007005A (es) | 2003-12-16 | 2008-02-14 | Monsanto Technology Llc | Composiciones de proteinas y genes secretados de bacillus thuringiensis y usos de las mismas. |
US20060021087A1 (en) | 2004-04-09 | 2006-01-26 | Baum James A | Compositions and methods for control of insect infestations in plants |
US8609936B2 (en) | 2007-04-27 | 2013-12-17 | Monsanto Technology Llc | Hemipteran-and coleopteran active toxin proteins from Bacillus thuringiensis |
CN110734919A (zh) * | 2008-07-02 | 2020-01-31 | 阿森尼克斯公司 | Axmi-il5、axmi-113、axmi-005、axmi-163和axmi-184∶vip3a杀虫蛋白及其使用方法 |
US8513493B2 (en) * | 2008-08-29 | 2013-08-20 | Monsanto Technology Llc | Hemipteran and coleopteran active toxin proteins from Bacillus thuringiensis |
US20100199383A1 (en) * | 2008-09-18 | 2010-08-05 | Pioneer Hi-Bred International, Inc. | Novel Bacillus Thuringiensis Gene with Coleopteran Activity |
JP5746055B2 (ja) * | 2009-02-27 | 2015-07-08 | アテニックス・コーポレーションAthenix Corporaton | 殺虫性タンパク質及びその使用方法 |
UA111592C2 (uk) * | 2010-07-07 | 2016-05-25 | Сінгента Партісіпейшнс Аг | Спосіб контролю над твердокрилими комахами-шкідниками |
UY33851A (es) * | 2010-12-30 | 2012-07-31 | Dow Agrosciences Llc | Moléculas de ácido nucleico que confieren resistencia a las plagas de coleópteros |
BRPI1107332A2 (pt) * | 2010-12-30 | 2018-10-30 | Dow Agrosciences Llc | polinucleotídeo, vetor de transformação, moléculas de ácido nucleico, célula, bem como métodos para controle de praga coleóptera, para aperfeiçoamento do rendimento de cultura de milho, e para produção de célula e planta resistentes à praga coleóptera |
WO2012109430A2 (en) | 2011-02-11 | 2012-08-16 | Monsanto Technology Llc | Pesticidal nucleic acids and proteins and uses thereof |
CN102399731A (zh) * | 2011-12-12 | 2012-04-04 | 西南大学 | 一株用于防治马铃薯甲虫的苏云金芽孢杆菌 |
CN102533841B (zh) * | 2012-02-13 | 2013-06-05 | 中国农业科学院生物技术研究所 | 一种提高Bt杀虫蛋白在汉逊酵母中表达量的方法 |
MD4196C1 (ro) * | 2012-04-27 | 2013-09-30 | Институт Зоологии Академии Наук Молдовы | Tulpină de bacterii Bacillus thuringiensis subsp. kurstaki -bioinsecticid pentru combaterea coleopterelor curculionoide |
CN102786584B (zh) * | 2012-08-02 | 2013-12-18 | 北京大北农科技集团股份有限公司 | 杀虫蛋白质、其编码基因及用途 |
CN102796183B (zh) * | 2012-08-02 | 2013-12-18 | 北京大北农科技集团股份有限公司 | 杀虫蛋白质、其编码基因及用途 |
CN102796182B (zh) * | 2012-08-02 | 2013-12-18 | 北京大北农科技集团股份有限公司 | 杀虫蛋白质、其编码基因及用途 |
US9783820B2 (en) | 2012-10-15 | 2017-10-10 | Pioneer Hi-Bred International, Inc. | Methods and compositions to enhance activity of Cry endotoxins |
WO2014071182A1 (en) | 2012-11-01 | 2014-05-08 | Massachusetts Institute Of Technology | Directed evolution of synthetic gene cluster |
EP3744727A1 (en) | 2013-03-14 | 2020-12-02 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
EP2971000A4 (en) | 2013-03-15 | 2016-11-23 | Pioneer Hi Bred Int | PHI-4 POLYPEPTIDES AND METHOD FOR THEIR USE |
WO2015021354A2 (en) * | 2013-08-09 | 2015-02-12 | Monsanto Technology Llc | Pesticidal toxin active against coleopteran and/or hemipteran insects |
EA030896B1 (ru) | 2013-08-16 | 2018-10-31 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
BR122021005579B1 (pt) | 2013-09-13 | 2022-11-29 | Pioneer Hi-Bred International, Inc | Construto de dna, método de obtenção de planta transgênica, proteína de fusão, método para controlar uma população de praga de inseto, método para inibir o crescimento ou matar uma praga de inseto |
CN114763376A (zh) | 2014-02-07 | 2022-07-19 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
CN117903266A (zh) | 2014-02-07 | 2024-04-19 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
CN103814956B (zh) * | 2014-03-04 | 2015-12-09 | 中南林业科技大学 | 油茶饼粕提取物与苏云金杆菌复配杀虫剂及其制备方法 |
EP3122882A4 (en) * | 2014-03-28 | 2018-02-28 | Monsanto Technology LLC | Pesticidal toxin proteins active against coleopteran insects |
WO2016000237A1 (en) | 2014-07-03 | 2016-01-07 | Pioneer Overseas Corporation | Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes |
WO2016044092A1 (en) | 2014-09-17 | 2016-03-24 | Pioneer Hi Bred International Inc | Compositions and methods to control insect pests |
CN106795525B (zh) * | 2014-10-15 | 2021-07-30 | 先锋国际良种公司 | 具有广谱活性的杀昆虫多肽及其用途 |
BR112017007932A2 (pt) * | 2014-10-16 | 2018-01-23 | Du Pont | proteínas inseticidas e métodos para uso das mesmas |
EP3207050A4 (en) * | 2014-10-16 | 2018-09-12 | Pioneer Hi-Bred International, Inc. | Insecticidal polypeptides having improved activity spectrum and uses thereof |
MX2017011525A (es) | 2015-03-11 | 2018-01-30 | Pioneer Hi Bred Int | Combinaciones insecticidas de pip-72 y metodos de uso. |
CA2985198A1 (en) | 2015-05-19 | 2016-11-24 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CN107771181A (zh) | 2015-06-16 | 2018-03-06 | 先锋国际良种公司 | 用以防治昆虫有害生物的组合物和方法 |
BR112017027821A2 (pt) * | 2015-06-22 | 2019-05-14 | AgBiome, Inc. | genes pesticidas e métodos de uso |
KR102461443B1 (ko) | 2015-07-13 | 2022-10-31 | 피벗 바이오, 인크. | 식물 형질 개선을 위한 방법 및 조성물 |
JP6933641B2 (ja) * | 2015-07-30 | 2021-09-08 | モンサント テクノロジー エルエルシー | 新規の昆虫阻害タンパク質 |
MX2018001523A (es) | 2015-08-06 | 2018-03-15 | Pioneer Hi Bred Int | Proteinas insecticidas derivadas de plantas y metodos para su uso. |
ES2773072T3 (es) | 2015-08-28 | 2020-07-09 | Pioneer Hi Bred Int | Transformación de plantas mediada por Ochrobactrum |
EP3359664A4 (en) | 2015-10-05 | 2019-03-20 | Massachusetts Institute Of Technology | NITROGEN FIXATION WITH REINFORCED NIF CLUSTERS |
WO2017105987A1 (en) | 2015-12-18 | 2017-06-22 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
UA126966C2 (uk) | 2016-04-14 | 2023-03-01 | Піонір Хай-Бред Інтернешнл, Інк. | Інсектицидний поліпептид, що характеризується поліпшеним спектром активності, та його застосування |
BR112018071502A2 (pt) | 2016-04-19 | 2019-04-30 | Pioneer Hi-Bred International, Inc. | construção de dna, planta transgênica ou descendente da mesma, composição e método para controlar uma população de pragas de insetos |
WO2017192560A1 (en) | 2016-05-04 | 2017-11-09 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
BR112018076047A2 (pt) | 2016-06-16 | 2019-03-26 | Pioneer Hi Bred Int | elemento de silenciamento, construto de dna, cassete de expressão, célula hospedeira, composição, célula vegetal, planta ou parte de planta, semente transgênica, método para controlar um inseto-praga de planta e kit |
CN116334123A (zh) | 2016-06-24 | 2023-06-27 | 先锋国际良种公司 | 植物调节元件及其使用方法 |
EP3954202A1 (en) | 2016-07-01 | 2022-02-16 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
US20210292778A1 (en) | 2016-07-12 | 2021-09-23 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
AR109205A1 (es) | 2016-08-05 | 2018-11-07 | Syngenta Participations Ag | Control de plagas de coleópteros utilizando moléculas de arn |
CN111196844B (zh) * | 2016-09-21 | 2021-11-30 | 中国农业科学院植物保护研究所 | 一种杀虫蛋白及其核酸、制备方法和应用 |
US11021716B2 (en) | 2016-11-01 | 2021-06-01 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CA3044404A1 (en) | 2016-12-14 | 2018-06-21 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2018118811A1 (en) | 2016-12-22 | 2018-06-28 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CA3049258A1 (en) | 2017-01-12 | 2018-07-19 | Pivot Bio, Inc. | Methods and compositions for improving plant traits |
WO2018140214A1 (en) | 2017-01-24 | 2018-08-02 | Pioneer Hi-Bred International, Inc. | Nematicidal protein from pseudomonas |
WO2018148001A1 (en) | 2017-02-08 | 2018-08-16 | Pioneer Hi-Bred International Inc | Insecticidal combinations of plant derived insecticidal proteins and methods for their use |
EA201992824A1 (ru) | 2017-05-26 | 2020-05-06 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные полипептиды, обладающие улучшенным спектром активности, и пути их применения |
WO2019074598A1 (en) | 2017-10-13 | 2019-04-18 | Pioneer Hi-Bred International, Inc. | VIRUS-INDUCED GENETIC SILENCING TECHNOLOGY FOR THE CONTROL OF INSECTS IN MAIZE |
EP3755797A1 (en) | 2018-02-22 | 2020-12-30 | Zymergen, Inc. | Method for creating a genomic library enriched for bacillus and identification of novel cry toxins |
CA3087861A1 (en) | 2018-03-02 | 2019-09-06 | Pioneer Hi-Bred International, Inc. | Plant health assay |
CA3088011A1 (en) | 2018-03-02 | 2019-09-06 | Zymergen Inc. | Insecticidal protein discovery platform and insecticidal proteins discovered therefrom |
CA3096516A1 (en) | 2018-05-22 | 2019-11-28 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
WO2020006064A2 (en) | 2018-06-27 | 2020-01-02 | Pivot Bio, Inc. | Agricultural compositions comprising remodeled nitrogen fixing microbes |
CN112689677A (zh) | 2018-08-29 | 2021-04-20 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
CN112867796A (zh) * | 2018-10-10 | 2021-05-28 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
BR112021011548A2 (pt) * | 2018-12-17 | 2021-10-26 | Syngenta Crop Protection Ag | Proteínas inseticidas |
WO2021076346A1 (en) | 2019-10-18 | 2021-04-22 | Pioneer Hi-Bred International, Inc. | Maize event dp-202216-6 and dp-023211-2 stack |
TW202142114A (zh) | 2020-02-04 | 2021-11-16 | 美商陶氏農業科學公司 | 具有殺有害生物效用之組成物及與其相關之方法 |
US20230235352A1 (en) | 2020-07-14 | 2023-07-27 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
US20230348928A1 (en) | 2020-08-10 | 2023-11-02 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
CN112480219B (zh) * | 2020-11-30 | 2021-10-12 | 东北农业大学 | Sip1Aa可溶性杀虫蛋白突变体 |
CN112480220B (zh) * | 2020-12-02 | 2021-10-12 | 东北农业大学 | 苏云金芽胞杆菌Sip1Aa蛋白随机重组突变蛋白 |
TW202345696A (zh) | 2022-05-18 | 2023-12-01 | 美商科迪華農業科技有限責任公司 | 具有殺有害生物效用之組成物及與其相關的方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR8600161A (pt) | 1985-01-18 | 1986-09-23 | Plant Genetic Systems Nv | Gene quimerico,vetores de plasmidio hibrido,intermediario,processo para controlar insetos em agricultura ou horticultura,composicao inseticida,processo para transformar celulas de plantas para expressar uma toxina de polipeptideo produzida por bacillus thuringiensis,planta,semente de planta,cultura de celulas e plasmidio |
US5608142A (en) * | 1986-12-03 | 1997-03-04 | Agracetus, Inc. | Insecticidal cotton plants |
US5024837A (en) | 1987-05-06 | 1991-06-18 | Donovan William P | Coleopteran active microorganisms, related insecticide compositions and methods for their production and use |
GB8910624D0 (en) * | 1989-05-09 | 1989-06-21 | Ici Plc | Bacterial strains |
US5308760A (en) * | 1992-01-10 | 1994-05-03 | Washington Research Foundation | Crystal proteins of Bacillus thuringiensis, genes encoding them, and host expressing them |
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AU2004267355B2 (en) | 2010-04-08 |
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