ZA200505589B - DNA molecules encoding a cotton chitinase and promoter - Google Patents
DNA molecules encoding a cotton chitinase and promoter Download PDFInfo
- Publication number
- ZA200505589B ZA200505589B ZA200505589A ZA200505589A ZA200505589B ZA 200505589 B ZA200505589 B ZA 200505589B ZA 200505589 A ZA200505589 A ZA 200505589A ZA 200505589 A ZA200505589 A ZA 200505589A ZA 200505589 B ZA200505589 B ZA 200505589B
- Authority
- ZA
- South Africa
- Prior art keywords
- plant
- cotton
- dna
- fiber
- promoter
- Prior art date
Links
- 229920000742 Cotton Polymers 0.000 title claims abstract description 236
- 108010022172 Chitinases Proteins 0.000 title claims abstract description 233
- 102000012286 Chitinases Human genes 0.000 title claims abstract description 228
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 304
- 230000014509 gene expression Effects 0.000 claims abstract description 254
- 239000000835 fiber Substances 0.000 claims abstract description 210
- 229920002678 cellulose Polymers 0.000 claims abstract description 99
- 239000001913 cellulose Substances 0.000 claims abstract description 99
- 230000008021 deposition Effects 0.000 claims abstract description 97
- 230000001105 regulatory effect Effects 0.000 claims abstract description 94
- 238000000034 method Methods 0.000 claims abstract description 54
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 40
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 40
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 40
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 18
- 229920001184 polypeptide Polymers 0.000 claims abstract description 16
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 16
- 241000196324 Embryophyta Species 0.000 claims description 295
- 210000004027 cell Anatomy 0.000 claims description 180
- 108020004414 DNA Proteins 0.000 claims description 163
- 241000219146 Gossypium Species 0.000 claims description 107
- 102000004169 proteins and genes Human genes 0.000 claims description 106
- 230000000694 effects Effects 0.000 claims description 67
- 210000002421 cell wall Anatomy 0.000 claims description 65
- 230000015572 biosynthetic process Effects 0.000 claims description 59
- 230000027455 binding Effects 0.000 claims description 43
- 238000003786 synthesis reaction Methods 0.000 claims description 39
- 238000013518 transcription Methods 0.000 claims description 32
- 230000035897 transcription Effects 0.000 claims description 32
- 239000002773 nucleotide Substances 0.000 claims description 31
- 125000003729 nucleotide group Chemical group 0.000 claims description 31
- 102000004190 Enzymes Human genes 0.000 claims description 30
- 108090000790 Enzymes Proteins 0.000 claims description 30
- 239000004753 textile Substances 0.000 claims description 25
- 244000299507 Gossypium hirsutum Species 0.000 claims description 19
- 230000008859 change Effects 0.000 claims description 19
- 230000012010 growth Effects 0.000 claims description 18
- 240000008042 Zea mays Species 0.000 claims description 17
- 230000001965 increasing effect Effects 0.000 claims description 16
- 102000040945 Transcription factor Human genes 0.000 claims description 15
- 108091023040 Transcription factor Proteins 0.000 claims description 15
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 14
- 102000053602 DNA Human genes 0.000 claims description 13
- 240000007594 Oryza sativa Species 0.000 claims description 13
- 235000007164 Oryza sativa Nutrition 0.000 claims description 13
- 108010040093 cellulose synthase Proteins 0.000 claims description 13
- 235000009566 rice Nutrition 0.000 claims description 13
- 229920000642 polymer Polymers 0.000 claims description 11
- 240000000111 Saccharum officinarum Species 0.000 claims description 10
- 235000007201 Saccharum officinarum Nutrition 0.000 claims description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 10
- 230000001580 bacterial effect Effects 0.000 claims description 10
- 235000005822 corn Nutrition 0.000 claims description 10
- 230000001131 transforming effect Effects 0.000 claims description 10
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 9
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 9
- 240000008564 Boehmeria nivea Species 0.000 claims description 9
- 244000025254 Cannabis sativa Species 0.000 claims description 9
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 claims description 9
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 claims description 9
- 244000146553 Ceiba pentandra Species 0.000 claims description 9
- 235000003301 Ceiba pentandra Nutrition 0.000 claims description 9
- 240000000491 Corchorus aestuans Species 0.000 claims description 9
- 235000011777 Corchorus aestuans Nutrition 0.000 claims description 9
- 235000010862 Corchorus capsularis Nutrition 0.000 claims description 9
- 240000000797 Hibiscus cannabinus Species 0.000 claims description 9
- 235000004431 Linum usitatissimum Nutrition 0.000 claims description 9
- 240000006240 Linum usitatissimum Species 0.000 claims description 9
- 240000000907 Musa textilis Species 0.000 claims description 9
- 244000082204 Phyllostachys viridis Species 0.000 claims description 9
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 9
- 108010043934 Sucrose synthase Proteins 0.000 claims description 9
- 241000244317 Tillandsia usneoides Species 0.000 claims description 9
- 239000011425 bamboo Substances 0.000 claims description 9
- 235000009120 camo Nutrition 0.000 claims description 9
- 235000005607 chanvre indien Nutrition 0.000 claims description 9
- 239000004459 forage Substances 0.000 claims description 9
- 239000011487 hemp Substances 0.000 claims description 9
- 241000589158 Agrobacterium Species 0.000 claims description 8
- 108700006291 Sucrose-phosphate synthases Proteins 0.000 claims description 8
- 150000002632 lipids Chemical class 0.000 claims description 8
- 230000003436 cytoskeletal effect Effects 0.000 claims description 7
- 239000011159 matrix material Substances 0.000 claims description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052799 carbon Inorganic materials 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 6
- 229920003023 plastic Polymers 0.000 claims description 6
- 229920001282 polysaccharide Polymers 0.000 claims description 5
- 239000005017 polysaccharide Substances 0.000 claims description 5
- 230000000717 retained effect Effects 0.000 claims description 5
- 238000000638 solvent extraction Methods 0.000 claims description 5
- 102000048175 UTP-glucose-1-phosphate uridylyltransferases Human genes 0.000 claims description 4
- 102000034356 gene-regulatory proteins Human genes 0.000 claims description 4
- 108091006104 gene-regulatory proteins Proteins 0.000 claims description 4
- 230000019491 signal transduction Effects 0.000 claims description 4
- 150000001720 carbohydrates Chemical class 0.000 claims description 3
- 235000014633 carbohydrates Nutrition 0.000 claims description 3
- 239000003375 plant hormone Substances 0.000 claims description 3
- 230000002194 synthesizing effect Effects 0.000 claims description 3
- 102000005548 Hexokinase Human genes 0.000 claims description 2
- 108700040460 Hexokinases Proteins 0.000 claims description 2
- 108700023183 UTP-glucose-1-phosphate uridylyltransferases Proteins 0.000 claims description 2
- 108010051210 beta-Fructofuranosidase Proteins 0.000 claims description 2
- 230000001851 biosynthetic effect Effects 0.000 claims description 2
- 230000003247 decreasing effect Effects 0.000 claims description 2
- 235000011073 invertase Nutrition 0.000 claims description 2
- 244000193174 agave Species 0.000 claims 4
- 150000004676 glycans Chemical class 0.000 claims 1
- 241000233866 Fungi Species 0.000 abstract description 13
- 241000238631 Hexapoda Species 0.000 abstract description 9
- 210000001519 tissue Anatomy 0.000 description 145
- 235000018102 proteins Nutrition 0.000 description 99
- 235000001014 amino acid Nutrition 0.000 description 72
- 229940024606 amino acid Drugs 0.000 description 70
- 150000001413 amino acids Chemical class 0.000 description 66
- 238000011161 development Methods 0.000 description 66
- 230000018109 developmental process Effects 0.000 description 63
- 241000219194 Arabidopsis Species 0.000 description 61
- 229920002101 Chitin Polymers 0.000 description 40
- 238000012360 testing method Methods 0.000 description 38
- 230000009466 transformation Effects 0.000 description 37
- 108091035707 Consensus sequence Proteins 0.000 description 32
- FVFVNNKYKYZTJU-UHFFFAOYSA-N 6-chloro-1,3,5-triazine-2,4-diamine Chemical compound NC1=NC(N)=NC(Cl)=N1 FVFVNNKYKYZTJU-UHFFFAOYSA-N 0.000 description 31
- 230000009261 transgenic effect Effects 0.000 description 31
- 239000012634 fragment Substances 0.000 description 30
- 239000002299 complementary DNA Substances 0.000 description 29
- 230000006870 function Effects 0.000 description 29
- 244000061176 Nicotiana tabacum Species 0.000 description 28
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 28
- 125000003275 alpha amino acid group Chemical group 0.000 description 28
- 229940088598 enzyme Drugs 0.000 description 26
- 239000013598 vector Substances 0.000 description 25
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 24
- 239000002609 medium Substances 0.000 description 22
- 230000006872 improvement Effects 0.000 description 21
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 20
- 229930027917 kanamycin Natural products 0.000 description 20
- 229960000318 kanamycin Drugs 0.000 description 20
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 20
- 229930182823 kanamycin A Natural products 0.000 description 20
- 108020004999 messenger RNA Proteins 0.000 description 18
- 238000012545 processing Methods 0.000 description 18
- 238000006467 substitution reaction Methods 0.000 description 18
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical group CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 17
- 230000003197 catalytic effect Effects 0.000 description 17
- 230000035772 mutation Effects 0.000 description 17
- 230000037039 plant physiology Effects 0.000 description 17
- 241000894007 species Species 0.000 description 17
- 239000000758 substrate Substances 0.000 description 17
- 241000490494 Arabis Species 0.000 description 16
- 230000033228 biological regulation Effects 0.000 description 16
- 238000004458 analytical method Methods 0.000 description 15
- 238000003780 insertion Methods 0.000 description 15
- 230000037431 insertion Effects 0.000 description 15
- 230000003993 interaction Effects 0.000 description 15
- 210000001161 mammalian embryo Anatomy 0.000 description 15
- 229950006780 n-acetylglucosamine Drugs 0.000 description 15
- 239000013612 plasmid Substances 0.000 description 15
- 239000000523 sample Substances 0.000 description 15
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 14
- 230000000875 corresponding effect Effects 0.000 description 14
- 238000011144 upstream manufacturing Methods 0.000 description 14
- 235000009432 Gossypium hirsutum Nutrition 0.000 description 13
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 13
- 238000000636 Northern blotting Methods 0.000 description 13
- 230000000977 initiatory effect Effects 0.000 description 13
- 238000012163 sequencing technique Methods 0.000 description 13
- 206010020649 Hyperkeratosis Diseases 0.000 description 12
- 238000010353 genetic engineering Methods 0.000 description 12
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 12
- 229960004889 salicylic acid Drugs 0.000 description 12
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 11
- 240000003768 Solanum lycopersicum Species 0.000 description 11
- 230000008166 cellulose biosynthesis Effects 0.000 description 11
- 238000012512 characterization method Methods 0.000 description 11
- 230000002538 fungal effect Effects 0.000 description 11
- 238000011534 incubation Methods 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 11
- 244000000003 plant pathogen Species 0.000 description 11
- 210000001938 protoplast Anatomy 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 10
- 239000005977 Ethylene Substances 0.000 description 10
- 240000002024 Gossypium herbaceum Species 0.000 description 10
- 108010054251 arabinogalactan proteins Proteins 0.000 description 10
- 230000007123 defense Effects 0.000 description 10
- 229920005610 lignin Polymers 0.000 description 10
- 230000004048 modification Effects 0.000 description 10
- 238000012986 modification Methods 0.000 description 10
- 238000010186 staining Methods 0.000 description 10
- 230000007704 transition Effects 0.000 description 10
- UDPGUMQDCGORJQ-UHFFFAOYSA-N (2-chloroethyl)phosphonic acid Chemical compound OP(O)(=O)CCCl UDPGUMQDCGORJQ-UHFFFAOYSA-N 0.000 description 9
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 9
- 108091026890 Coding region Proteins 0.000 description 9
- 239000005976 Ethephon Substances 0.000 description 9
- 210000002257 embryonic structure Anatomy 0.000 description 9
- 239000000499 gel Substances 0.000 description 9
- 230000030279 gene silencing Effects 0.000 description 9
- 230000035479 physiological effects, processes and functions Effects 0.000 description 9
- 230000004044 response Effects 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- 235000010469 Glycine max Nutrition 0.000 description 8
- 244000068988 Glycine max Species 0.000 description 8
- 235000004341 Gossypium herbaceum Nutrition 0.000 description 8
- 241000218922 Magnoliophyta Species 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 8
- 230000004927 fusion Effects 0.000 description 8
- 238000009396 hybridization Methods 0.000 description 8
- 230000006698 induction Effects 0.000 description 8
- 210000001724 microfibril Anatomy 0.000 description 8
- 238000002703 mutagenesis Methods 0.000 description 8
- 231100000350 mutagenesis Toxicity 0.000 description 8
- 229920001542 oligosaccharide Polymers 0.000 description 8
- 239000002245 particle Substances 0.000 description 8
- 238000011069 regeneration method Methods 0.000 description 8
- 230000030118 somatic embryogenesis Effects 0.000 description 8
- 101710134123 Chitin-binding lectin Proteins 0.000 description 7
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 7
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 7
- 108700026226 TATA Box Proteins 0.000 description 7
- 230000001594 aberrant effect Effects 0.000 description 7
- 210000000349 chromosome Anatomy 0.000 description 7
- 238000010367 cloning Methods 0.000 description 7
- 230000009025 developmental regulation Effects 0.000 description 7
- 230000000366 juvenile effect Effects 0.000 description 7
- 230000031787 nutrient reservoir activity Effects 0.000 description 7
- 150000002482 oligosaccharides Chemical class 0.000 description 7
- 238000012261 overproduction Methods 0.000 description 7
- 230000002829 reductive effect Effects 0.000 description 7
- 230000008929 regeneration Effects 0.000 description 7
- 230000002441 reversible effect Effects 0.000 description 7
- 238000012546 transfer Methods 0.000 description 7
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Chemical compound CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 description 6
- 241000219195 Arabidopsis thaliana Species 0.000 description 6
- 108020004635 Complementary DNA Proteins 0.000 description 6
- 235000002767 Daucus carota Nutrition 0.000 description 6
- 244000000626 Daucus carota Species 0.000 description 6
- 108010044091 Globulins Proteins 0.000 description 6
- 230000004988 N-glycosylation Effects 0.000 description 6
- 108091092724 Noncoding DNA Proteins 0.000 description 6
- 102000009572 RNA Polymerase II Human genes 0.000 description 6
- 108010009460 RNA Polymerase II Proteins 0.000 description 6
- 108700008625 Reporter Genes Proteins 0.000 description 6
- 244000061456 Solanum tuberosum Species 0.000 description 6
- 230000019552 anatomical structure morphogenesis Effects 0.000 description 6
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 6
- 230000002950 deficient Effects 0.000 description 6
- 238000012217 deletion Methods 0.000 description 6
- 230000037430 deletion Effects 0.000 description 6
- 239000000284 extract Substances 0.000 description 6
- 150000002306 glutamic acid derivatives Chemical class 0.000 description 6
- 230000001939 inductive effect Effects 0.000 description 6
- 238000011068 loading method Methods 0.000 description 6
- 239000003550 marker Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 238000010369 molecular cloning Methods 0.000 description 6
- 210000000056 organ Anatomy 0.000 description 6
- 244000052769 pathogen Species 0.000 description 6
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 6
- 238000005204 segregation Methods 0.000 description 6
- 230000010474 transient expression Effects 0.000 description 6
- 230000002792 vascular Effects 0.000 description 6
- 240000004246 Agave americana Species 0.000 description 5
- 244000198134 Agave sisalana Species 0.000 description 5
- 108090000288 Glycoproteins Proteins 0.000 description 5
- 102000003886 Glycoproteins Human genes 0.000 description 5
- 102000004157 Hydrolases Human genes 0.000 description 5
- 108090000604 Hydrolases Proteins 0.000 description 5
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 5
- 206010028980 Neoplasm Diseases 0.000 description 5
- 244000046052 Phaseolus vulgaris Species 0.000 description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 description 5
- 229930006000 Sucrose Natural products 0.000 description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 5
- 230000009471 action Effects 0.000 description 5
- 238000013459 approach Methods 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 5
- 230000001413 cellular effect Effects 0.000 description 5
- 108010050949 chitinase C Proteins 0.000 description 5
- 238000006731 degradation reaction Methods 0.000 description 5
- 238000004520 electroporation Methods 0.000 description 5
- 101150054900 gus gene Proteins 0.000 description 5
- 238000003306 harvesting Methods 0.000 description 5
- 230000001976 improved effect Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 230000001717 pathogenic effect Effects 0.000 description 5
- 230000010287 polarization Effects 0.000 description 5
- 108010010718 poly(3-hydroxyalkanoic acid) synthase Proteins 0.000 description 5
- 238000000751 protein extraction Methods 0.000 description 5
- 238000004537 pulping Methods 0.000 description 5
- 108091008146 restriction endonucleases Proteins 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000005720 sucrose Substances 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 238000011282 treatment Methods 0.000 description 5
- 108020004463 18S ribosomal RNA Proteins 0.000 description 4
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 102100024342 Contactin-2 Human genes 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 4
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 4
- 229920001503 Glucan Polymers 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 229920002488 Hemicellulose Polymers 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- 102000005877 Peptide Initiation Factors Human genes 0.000 description 4
- 108010044843 Peptide Initiation Factors Proteins 0.000 description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 4
- 239000002202 Polyethylene glycol Substances 0.000 description 4
- 101710156592 Putative TATA-binding protein pB263R Proteins 0.000 description 4
- 230000004570 RNA-binding Effects 0.000 description 4
- 241000589180 Rhizobium Species 0.000 description 4
- 101000690440 Solanum lycopersicum Floral homeotic protein AGAMOUS Proteins 0.000 description 4
- 102100040296 TATA-box-binding protein Human genes 0.000 description 4
- 101710145783 TATA-box-binding protein Proteins 0.000 description 4
- HSCJRCZFDFQWRP-JZMIEXBBSA-N UDP-alpha-D-glucose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-JZMIEXBBSA-N 0.000 description 4
- 108091023045 Untranslated Region Proteins 0.000 description 4
- HSCJRCZFDFQWRP-UHFFFAOYSA-N Uridindiphosphoglukose Natural products OC1C(O)C(O)C(CO)OC1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-UHFFFAOYSA-N 0.000 description 4
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 4
- 108091000039 acetoacetyl-CoA reductase Proteins 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 230000008436 biogenesis Effects 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 230000013020 embryo development Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 210000001339 epidermal cell Anatomy 0.000 description 4
- 210000002615 epidermis Anatomy 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 238000012226 gene silencing method Methods 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 229920000140 heteropolymer Polymers 0.000 description 4
- 230000007062 hydrolysis Effects 0.000 description 4
- 238000006460 hydrolysis reaction Methods 0.000 description 4
- 230000002779 inactivation Effects 0.000 description 4
- 230000009545 invasion Effects 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 235000009973 maize Nutrition 0.000 description 4
- 230000001404 mediated effect Effects 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 230000036961 partial effect Effects 0.000 description 4
- 230000037361 pathway Effects 0.000 description 4
- 230000008635 plant growth Effects 0.000 description 4
- 229920001223 polyethylene glycol Polymers 0.000 description 4
- 150000004804 polysaccharides Chemical class 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000004445 quantitative analysis Methods 0.000 description 4
- 230000001850 reproductive effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 238000010561 standard procedure Methods 0.000 description 4
- 238000004114 suspension culture Methods 0.000 description 4
- 230000002123 temporal effect Effects 0.000 description 4
- 230000002103 transcriptional effect Effects 0.000 description 4
- 229960004441 tyrosine Drugs 0.000 description 4
- 210000005167 vascular cell Anatomy 0.000 description 4
- SGHZXLIDFTYFHQ-UHFFFAOYSA-L Brilliant Blue Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 SGHZXLIDFTYFHQ-UHFFFAOYSA-L 0.000 description 3
- 102100037084 C4b-binding protein alpha chain Human genes 0.000 description 3
- 208000005156 Dehydration Diseases 0.000 description 3
- 101100437498 Escherichia coli (strain K12) uidA gene Proteins 0.000 description 3
- 108091060211 Expressed sequence tag Proteins 0.000 description 3
- 229920002148 Gellan gum Polymers 0.000 description 3
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 3
- 235000009438 Gossypium Nutrition 0.000 description 3
- 101710144007 Mannose-1-phosphate guanyltransferase Proteins 0.000 description 3
- 108700020796 Oncogene Proteins 0.000 description 3
- 108700026244 Open Reading Frames Proteins 0.000 description 3
- 240000004713 Pisum sativum Species 0.000 description 3
- 235000010582 Pisum sativum Nutrition 0.000 description 3
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 3
- 101710136733 Proline-rich protein Proteins 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 238000002105 Southern blotting Methods 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 235000007244 Zea mays Nutrition 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 230000002060 circadian Effects 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 235000013922 glutamic acid Nutrition 0.000 description 3
- 239000004220 glutamic acid Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 230000004807 localization Effects 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 230000008121 plant development Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 230000008521 reorganization Effects 0.000 description 3
- QEVHRUUCFGRFIF-MDEJGZGSSA-N reserpine Chemical compound O([C@H]1[C@@H]([C@H]([C@H]2C[C@@H]3C4=C(C5=CC=C(OC)C=C5N4)CCN3C[C@H]2C1)C(=O)OC)OC)C(=O)C1=CC(OC)=C(OC)C(OC)=C1 QEVHRUUCFGRFIF-MDEJGZGSSA-N 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 230000000392 somatic effect Effects 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 230000008685 targeting Effects 0.000 description 3
- 230000001052 transient effect Effects 0.000 description 3
- 230000014616 translation Effects 0.000 description 3
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 3
- 239000002023 wood Substances 0.000 description 3
- HSHNITRMYYLLCV-UHFFFAOYSA-N 4-methylumbelliferone Chemical compound C1=C(O)C=CC2=C1OC(=O)C=C2C HSHNITRMYYLLCV-UHFFFAOYSA-N 0.000 description 2
- 102000007469 Actins Human genes 0.000 description 2
- 108010085238 Actins Proteins 0.000 description 2
- 101710146995 Acyl carrier protein Proteins 0.000 description 2
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 2
- 108700003860 Bacterial Genes Proteins 0.000 description 2
- 241001610429 Boechera microphylla Species 0.000 description 2
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 2
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 2
- 229920000018 Callose Polymers 0.000 description 2
- 241000701489 Cauliflower mosaic virus Species 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 2
- 241000819038 Chichester Species 0.000 description 2
- 235000009854 Cucurbita moschata Nutrition 0.000 description 2
- 240000001980 Cucurbita pepo Species 0.000 description 2
- 230000004568 DNA-binding Effects 0.000 description 2
- 206010017533 Fungal infection Diseases 0.000 description 2
- 102000030782 GTP binding Human genes 0.000 description 2
- 108091000058 GTP-Binding Proteins 0.000 description 2
- 240000000047 Gossypium barbadense Species 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
- 108090001060 Lipase Proteins 0.000 description 2
- 102000004882 Lipase Human genes 0.000 description 2
- 239000004367 Lipase Substances 0.000 description 2
- 108010014251 Muramidase Proteins 0.000 description 2
- 102000016943 Muramidase Human genes 0.000 description 2
- 208000031888 Mycoses Diseases 0.000 description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
- ZRWPUFFVAOMMNM-UHFFFAOYSA-N Patulin Chemical compound OC1OCC=C2OC(=O)C=C12 ZRWPUFFVAOMMNM-UHFFFAOYSA-N 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- 101710167959 Putative UTP-glucose-1-phosphate uridylyltransferase Proteins 0.000 description 2
- 241000589157 Rhizobiales Species 0.000 description 2
- 244000007853 Sarothamnus scoparius Species 0.000 description 2
- 235000002597 Solanum melongena Nutrition 0.000 description 2
- 244000061458 Solanum melongena Species 0.000 description 2
- 240000006394 Sorghum bicolor Species 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- 235000009337 Spinacia oleracea Nutrition 0.000 description 2
- 244000300264 Spinacia oleracea Species 0.000 description 2
- 108091081024 Start codon Proteins 0.000 description 2
- 229930183415 Suberin Natural products 0.000 description 2
- 101710156615 Sucrose synthase 1 Proteins 0.000 description 2
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 2
- 239000004473 Threonine Substances 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- 102000006290 Transcription Factor TFIID Human genes 0.000 description 2
- 108010083268 Transcription Factor TFIID Proteins 0.000 description 2
- 108090000941 Transcription factor TFIIB Proteins 0.000 description 2
- 102000004408 Transcription factor TFIIB Human genes 0.000 description 2
- 108700019146 Transgenes Proteins 0.000 description 2
- 102000011731 Vacuolar Proton-Translocating ATPases Human genes 0.000 description 2
- 108010037026 Vacuolar Proton-Translocating ATPases Proteins 0.000 description 2
- 108010046516 Wheat Germ Agglutinins Proteins 0.000 description 2
- 230000006578 abscission Effects 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 230000000843 anti-fungal effect Effects 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 229960001230 asparagine Drugs 0.000 description 2
- 235000009582 asparagine Nutrition 0.000 description 2
- 238000000376 autoradiography Methods 0.000 description 2
- 239000002363 auxin Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 108091008324 binding proteins Proteins 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 210000004899 c-terminal region Anatomy 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- AZZMGZXNTDTSME-JUZDKLSSSA-M cefotaxime sodium Chemical compound [Na+].N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C([O-])=O)=O)C(=O)\C(=N/OC)C1=CSC(N)=N1 AZZMGZXNTDTSME-JUZDKLSSSA-M 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 235000012343 cottonseed oil Nutrition 0.000 description 2
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 2
- 239000004062 cytokinin Substances 0.000 description 2
- 210000004292 cytoskeleton Anatomy 0.000 description 2
- 230000009274 differential gene expression Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000000408 embryogenic effect Effects 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 125000003147 glycosyl group Chemical group 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 229920001519 homopolymer Polymers 0.000 description 2
- 229920002674 hyaluronan Polymers 0.000 description 2
- 229940099552 hyaluronan Drugs 0.000 description 2
- KIUKXJAPPMFGSW-MNSSHETKSA-N hyaluronan Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)C1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H](C(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-MNSSHETKSA-N 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 2
- 239000000411 inducer Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 235000019421 lipase Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000004325 lysozyme Substances 0.000 description 2
- 229960000274 lysozyme Drugs 0.000 description 2
- 235000010335 lysozyme Nutrition 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 238000010208 microarray analysis Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 239000006870 ms-medium Substances 0.000 description 2
- 238000002887 multiple sequence alignment Methods 0.000 description 2
- 230000007498 myristoylation Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000024121 nodulation Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 230000026731 phosphorylation Effects 0.000 description 2
- 238000006366 phosphorylation reaction Methods 0.000 description 2
- 108010004568 plant pathogenesis-related proteins Proteins 0.000 description 2
- 230000004983 pleiotropic effect Effects 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000001124 posttranscriptional effect Effects 0.000 description 2
- 230000001323 posttranslational effect Effects 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 238000012794 pre-harvesting Methods 0.000 description 2
- 238000002203 pretreatment Methods 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000022869 regulation of cellulose biosynthetic process Effects 0.000 description 2
- 230000014493 regulation of gene expression Effects 0.000 description 2
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 2
- 102000037983 regulatory factors Human genes 0.000 description 2
- 108091008025 regulatory factors Proteins 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 230000009291 secondary effect Effects 0.000 description 2
- 238000002864 sequence alignment Methods 0.000 description 2
- 238000003196 serial analysis of gene expression Methods 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 230000011664 signaling Effects 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000004960 subcellular localization Effects 0.000 description 2
- 238000004809 thin layer chromatography Methods 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 239000001993 wax Substances 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 108010070892 1,3-beta-glucan synthase Proteins 0.000 description 1
- MVMSCBBUIHUTGJ-UHFFFAOYSA-N 10108-97-1 Natural products C1=2NC(N)=NC(=O)C=2N=CN1C(C(C1O)O)OC1COP(O)(=O)OP(O)(=O)OC1OC(CO)C(O)C(O)C1O MVMSCBBUIHUTGJ-UHFFFAOYSA-N 0.000 description 1
- YLFRRPUBVUAHSR-UHFFFAOYSA-N 16-dehydro-pregnenolone Natural products C1C=C2CC(O)CCC2(C)C2C1C1CC=C(C(=O)C)C1(C)CC2 YLFRRPUBVUAHSR-UHFFFAOYSA-N 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 1
- WZHMHOPKMPGQLO-UHFFFAOYSA-N 3-hydroxy-4-methylchromen-2-one Chemical compound C1=CC=CC2=C1OC(=O)C(O)=C2C WZHMHOPKMPGQLO-UHFFFAOYSA-N 0.000 description 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- YGUMVDWOQQJBGA-VAWYXSNFSA-N 5-[(4-anilino-6-morpholin-4-yl-1,3,5-triazin-2-yl)amino]-2-[(e)-2-[4-[(4-anilino-6-morpholin-4-yl-1,3,5-triazin-2-yl)amino]-2-sulfophenyl]ethenyl]benzenesulfonic acid Chemical compound C=1C=C(\C=C\C=2C(=CC(NC=3N=C(N=C(NC=4C=CC=CC=4)N=3)N3CCOCC3)=CC=2)S(O)(=O)=O)C(S(=O)(=O)O)=CC=1NC(N=C(N=1)N2CCOCC2)=NC=1NC1=CC=CC=C1 YGUMVDWOQQJBGA-VAWYXSNFSA-N 0.000 description 1
- CNGYZEMWVAWWOB-VAWYXSNFSA-N 5-[[4-anilino-6-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]-2-[(e)-2-[4-[[4-anilino-6-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]-2-sulfophenyl]ethenyl]benzenesulfonic acid Chemical compound N=1C(NC=2C=C(C(\C=C\C=3C(=CC(NC=4N=C(N=C(NC=5C=CC=CC=5)N=4)N(CCO)CCO)=CC=3)S(O)(=O)=O)=CC=2)S(O)(=O)=O)=NC(N(CCO)CCO)=NC=1NC1=CC=CC=C1 CNGYZEMWVAWWOB-VAWYXSNFSA-N 0.000 description 1
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- DHJFFLKPAYHPHU-UHFFFAOYSA-N 6-[(5-bromo-4-chloro-1h-indol-3-yl)oxy]-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(O)C(O)C(O)C1OC1=CNC2=CC=C(Br)C(Cl)=C12 DHJFFLKPAYHPHU-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 1
- 244000291564 Allium cepa Species 0.000 description 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
- 240000002234 Allium sativum Species 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 244000099147 Ananas comosus Species 0.000 description 1
- 235000007119 Ananas comosus Nutrition 0.000 description 1
- 102000000412 Annexin Human genes 0.000 description 1
- 108050008874 Annexin Proteins 0.000 description 1
- 240000007087 Apium graveolens Species 0.000 description 1
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
- 235000010591 Appio Nutrition 0.000 description 1
- 101100112410 Arabidopsis thaliana LHCB1.3 gene Proteins 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 102100023927 Asparagine synthetase [glutamine-hydrolyzing] Human genes 0.000 description 1
- 235000000832 Ayote Nutrition 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 238000009010 Bradford assay Methods 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 101100172879 Caenorhabditis elegans sec-5 gene Proteins 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 101000767750 Carya illinoinensis Vicilin Car i 2.0101 Proteins 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 240000006740 Cichorium endivia Species 0.000 description 1
- 235000007542 Cichorium intybus Nutrition 0.000 description 1
- 244000298479 Cichorium intybus Species 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- MFYSYFVPBJMHGN-UHFFFAOYSA-N Cortisone Natural products O=C1CCC2(C)C3C(=O)CC(C)(C(CC4)(O)C(=O)CO)C4C3CCC2=C1 MFYSYFVPBJMHGN-UHFFFAOYSA-N 0.000 description 1
- 101000767759 Corylus avellana Vicilin Cor a 11.0101 Proteins 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- 241000219130 Cucurbita pepo subsp. pepo Species 0.000 description 1
- 235000003954 Cucurbita pepo var melopepo Nutrition 0.000 description 1
- 229920000832 Cutin Polymers 0.000 description 1
- NBSCHQHZLSJFNQ-GASJEMHNSA-N D-Glucose 6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-GASJEMHNSA-N 0.000 description 1
- 101150074155 DHFR gene Proteins 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 230000005901 DNA-dependent transcriptional open complex formation Effects 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 101150105978 EP3 gene Proteins 0.000 description 1
- 102100031690 Erythroid transcription factor Human genes 0.000 description 1
- 101710100588 Erythroid transcription factor Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- 108050000194 Expansin Proteins 0.000 description 1
- 101710129170 Extensin Proteins 0.000 description 1
- 101710145505 Fiber protein Proteins 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- MVMSCBBUIHUTGJ-GDJBGNAASA-N GDP-alpha-D-mannose Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H]1O)O)N1C=2N=C(NC(=O)C=2N=C1)N)OP(O)(=O)OP(O)(=O)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]1O MVMSCBBUIHUTGJ-GDJBGNAASA-N 0.000 description 1
- 102000013446 GTP Phosphohydrolases Human genes 0.000 description 1
- 108091006109 GTPases Proteins 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- 102100033840 General transcription factor IIF subunit 1 Human genes 0.000 description 1
- 102100032863 General transcription factor IIH subunit 3 Human genes 0.000 description 1
- 229920002581 Glucomannan Polymers 0.000 description 1
- 108010060309 Glucuronidase Proteins 0.000 description 1
- 102000053187 Glucuronidase Human genes 0.000 description 1
- 229940121672 Glycosylation inhibitor Drugs 0.000 description 1
- 240000001814 Gossypium arboreum Species 0.000 description 1
- 235000000292 Gouania lupuloides Nutrition 0.000 description 1
- 244000299452 Gouania lupuloides Species 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 108700005087 Homeobox Genes Proteins 0.000 description 1
- 101100380329 Homo sapiens ASNS gene Proteins 0.000 description 1
- 101000666405 Homo sapiens General transcription factor IIH subunit 1 Proteins 0.000 description 1
- 101000655398 Homo sapiens General transcription factor IIH subunit 2 Proteins 0.000 description 1
- 101000655391 Homo sapiens General transcription factor IIH subunit 3 Proteins 0.000 description 1
- 101000655406 Homo sapiens General transcription factor IIH subunit 4 Proteins 0.000 description 1
- 101000655402 Homo sapiens General transcription factor IIH subunit 5 Proteins 0.000 description 1
- 101000740205 Homo sapiens Sal-like protein 1 Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 101000622316 Juglans regia Vicilin Jug r 2.0101 Proteins 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 241000446313 Lamella Species 0.000 description 1
- 240000006568 Lathyrus odoratus Species 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- DUKURNFHYQXCJG-UHFFFAOYSA-N Lewis A pentasaccharide Natural products OC1C(O)C(O)C(C)OC1OC1C(OC2C(C(O)C(O)C(CO)O2)O)C(NC(C)=O)C(OC2C(C(OC3C(OC(O)C(O)C3O)CO)OC(CO)C2O)O)OC1CO DUKURNFHYQXCJG-UHFFFAOYSA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 102000001845 Lipid-Linked Proteins Human genes 0.000 description 1
- 239000006142 Luria-Bertani Agar Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241000220225 Malus Species 0.000 description 1
- 235000011430 Malus pumila Nutrition 0.000 description 1
- 235000015103 Malus silvestris Nutrition 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 1
- 125000003686 N-acetyl-D-glucosaminyl group Chemical group C(C)(=O)N[C@H]1C(O[C@@H]([C@H]([C@@H]1O)O)CO)* 0.000 description 1
- MNLRQHMNZILYPY-MDMHTWEWSA-N N-acetyl-alpha-D-muramic acid Chemical compound OC(=O)[C@@H](C)O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O)[C@@H]1NC(C)=O MNLRQHMNZILYPY-MDMHTWEWSA-N 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 101710149663 Osmotin Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000222291 Passalora fulva Species 0.000 description 1
- 239000006002 Pepper Substances 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 108010013639 Peptidoglycan Proteins 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- IHPVFYLOGNNZLA-UHFFFAOYSA-N Phytoalexin Natural products COC1=CC=CC=C1C1OC(C=C2C(OCO2)=C2OC)=C2C(=O)C1 IHPVFYLOGNNZLA-UHFFFAOYSA-N 0.000 description 1
- 240000000020 Picea glauca Species 0.000 description 1
- 235000008127 Picea glauca Nutrition 0.000 description 1
- 241000364051 Pima Species 0.000 description 1
- 101000767757 Pinus koraiensis Vicilin Pin k 2.0101 Proteins 0.000 description 1
- 235000016761 Piper aduncum Nutrition 0.000 description 1
- 240000003889 Piper guineense Species 0.000 description 1
- 235000017804 Piper guineense Nutrition 0.000 description 1
- 235000008184 Piper nigrum Nutrition 0.000 description 1
- 101000767758 Pistacia vera Vicilin Pis v 3.0101 Proteins 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 101710096706 Poly(3-hydroxyalkanoate) polymerase Proteins 0.000 description 1
- 101710159749 Poly(3-hydroxyalkanoate) polymerase subunit PhaC Proteins 0.000 description 1
- 101710159752 Poly(3-hydroxyalkanoate) polymerase subunit PhaE Proteins 0.000 description 1
- 241001085205 Prenanthella exigua Species 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 1
- 108010029485 Protein Isoforms Proteins 0.000 description 1
- 102000001708 Protein Isoforms Human genes 0.000 description 1
- 102000001253 Protein Kinase Human genes 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 240000001987 Pyrus communis Species 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 239000003391 RNA probe Substances 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 108091030071 RNAI Proteins 0.000 description 1
- 101150075111 ROLB gene Proteins 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 240000007651 Rubus glaucus Species 0.000 description 1
- 235000011034 Rubus glaucus Nutrition 0.000 description 1
- 235000009122 Rubus idaeus Nutrition 0.000 description 1
- 102100037204 Sal-like protein 1 Human genes 0.000 description 1
- 241000209056 Secale Species 0.000 description 1
- 235000007238 Secale cereale Nutrition 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 102100038803 Somatotropin Human genes 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 101710136739 Teichoic acid poly(glycerol phosphate) polymerase Proteins 0.000 description 1
- 108010022394 Threonine synthase Proteins 0.000 description 1
- 241000723873 Tobacco mosaic virus Species 0.000 description 1
- 108010083262 Transcription Factor TFIIA Proteins 0.000 description 1
- 102000006289 Transcription Factor TFIIA Human genes 0.000 description 1
- 108700009124 Transcription Initiation Site Proteins 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- YJQCOFNZVFGCAF-UHFFFAOYSA-N Tunicamycin II Natural products O1C(CC(O)C2C(C(O)C(O2)N2C(NC(=O)C=C2)=O)O)C(O)C(O)C(NC(=O)C=CCCCCCCCCC(C)C)C1OC1OC(CO)C(O)C(O)C1NC(C)=O YJQCOFNZVFGCAF-UHFFFAOYSA-N 0.000 description 1
- 241001106462 Ulmus Species 0.000 description 1
- 240000006064 Urena lobata Species 0.000 description 1
- 241001123668 Verticillium dahliae Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 229920002522 Wood fibre Polymers 0.000 description 1
- 229920002000 Xyloglucan Polymers 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 208000005652 acute fatty liver of pregnancy Diseases 0.000 description 1
- 230000010933 acylation Effects 0.000 description 1
- 238000005917 acylation reaction Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 1
- HXXFSFRBOHSIMQ-RWOPYEJCSA-L alpha-D-mannose 1-phosphate(2-) Chemical compound OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@@H](O)[C@@H](O)[C@@H]1O HXXFSFRBOHSIMQ-RWOPYEJCSA-L 0.000 description 1
- 230000009435 amidation Effects 0.000 description 1
- 238000007112 amidation reaction Methods 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- IYIKLHRQXLHMJQ-UHFFFAOYSA-N amiodarone Chemical compound CCCCC=1OC2=CC=CC=C2C=1C(=O)C1=CC(I)=C(OCCN(CC)CC)C(I)=C1 IYIKLHRQXLHMJQ-UHFFFAOYSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 101150026213 atpB gene Proteins 0.000 description 1
- 230000003816 axenic effect Effects 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 230000008238 biochemical pathway Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 230000007321 biological mechanism Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000009924 canning Methods 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 229960004261 cefotaxime Drugs 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 108091008394 cellulose binding proteins Proteins 0.000 description 1
- 239000012707 chemical precursor Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 235000003733 chicria Nutrition 0.000 description 1
- 230000001794 chitinolytic effect Effects 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- 229930002868 chlorophyll a Natural products 0.000 description 1
- 229930002869 chlorophyll b Natural products 0.000 description 1
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 229940088530 claforan Drugs 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000002153 concerted effect Effects 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000001054 cortical effect Effects 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000002178 crystalline material Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- PAFZNILMFXTMIY-UHFFFAOYSA-O cyclohexylammonium Chemical class [NH3+]C1CCCCC1 PAFZNILMFXTMIY-UHFFFAOYSA-O 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000004665 defense response Effects 0.000 description 1
- 230000003413 degradative effect Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000001152 differential interference contrast microscopy Methods 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 235000005489 dwarf bean Nutrition 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 239000005712 elicitor Substances 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 125000001924 fatty-acyl group Chemical group 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 108010060641 flavanone synthetase Proteins 0.000 description 1
- 230000004883 flower formation Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 235000004611 garlic Nutrition 0.000 description 1
- 230000009368 gene silencing by RNA Effects 0.000 description 1
- 229960002442 glucosamine Drugs 0.000 description 1
- 229940097043 glucuronic acid Drugs 0.000 description 1
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 238000007825 histological assay Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000010249 in-situ analysis Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 229940097275 indigo Drugs 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- GSXOAOHZAIYLCY-HSUXUTPPSA-N keto-D-fructose 6-phosphate Chemical compound OCC(=O)[C@@H](O)[C@H](O)[C@H](O)COP(O)(O)=O GSXOAOHZAIYLCY-HSUXUTPPSA-N 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
- 229960001669 kinetin Drugs 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 108010053156 lipid transfer protein Proteins 0.000 description 1
- 108091005630 lipid-anchored proteins Proteins 0.000 description 1
- 150000002634 lipophilic molecules Chemical class 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 210000003712 lysosome Anatomy 0.000 description 1
- 230000001868 lysosomic effect Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 230000023409 microsporogenesis Effects 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 229940105132 myristate Drugs 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 101150105920 npr gene Proteins 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 230000025342 organ morphogenesis Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 101150077062 pal gene Proteins 0.000 description 1
- 239000000123 paper Substances 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 239000000280 phytoalexin Substances 0.000 description 1
- 150000001857 phytoalexin derivatives Chemical class 0.000 description 1
- 239000008104 plant cellulose Substances 0.000 description 1
- 210000002706 plastid Anatomy 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 230000009290 primary effect Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001915 proofreading effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000004224 protection Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 238000000734 protein sequencing Methods 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 238000000575 proteomic method Methods 0.000 description 1
- 235000015136 pumpkin Nutrition 0.000 description 1
- 238000012207 quantitative assay Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 101150036680 rav1 gene Proteins 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 102220221032 rs61729796 Human genes 0.000 description 1
- 230000021217 seedling development Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 235000020354 squash Nutrition 0.000 description 1
- 230000010473 stable expression Effects 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 238000012731 temporal analysis Methods 0.000 description 1
- TUNFSRHWOTWDNC-UHFFFAOYSA-N tetradecanoic acid Chemical compound CCCCCCCCCCCCCC(O)=O TUNFSRHWOTWDNC-UHFFFAOYSA-N 0.000 description 1
- 229920001169 thermoplastic Polymers 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 108010014677 transcription factor TFIIE Proteins 0.000 description 1
- 108010014678 transcription factor TFIIF Proteins 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 238000005918 transglycosylation reaction Methods 0.000 description 1
- 230000005068 transpiration Effects 0.000 description 1
- ZHSGGJXRNHWHRS-VIDYELAYSA-N tunicamycin Chemical compound O([C@H]1[C@@H]([C@H]([C@@H](O)[C@@H](CC(O)[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C(NC(=O)C=C2)=O)O)O1)O)NC(=O)/C=C/CC(C)C)[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1NC(C)=O ZHSGGJXRNHWHRS-VIDYELAYSA-N 0.000 description 1
- MEYZYGMYMLNUHJ-UHFFFAOYSA-N tunicamycin Natural products CC(C)CCCCCCCCCC=CC(=O)NC1C(O)C(O)C(CC(O)C2OC(C(O)C2O)N3C=CC(=O)NC3=O)OC1OC4OC(CO)C(O)C(O)C4NC(=O)C MEYZYGMYMLNUHJ-UHFFFAOYSA-N 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 238000013022 venting Methods 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 229920001221 xylan Polymers 0.000 description 1
- 150000004823 xylans Chemical class 0.000 description 1
- 230000004572 zinc-binding Effects 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2442—Chitinase (3.2.1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01014—Chitinase (3.2.1.14)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/350,696 US7098324B2 (en) | 2001-07-30 | 2003-01-23 | Chitinase encoding DNA molecules from cotton expressed preferentially in secondary walled cells during secondary wall deposition and a corresponding promoter |
Publications (1)
Publication Number | Publication Date |
---|---|
ZA200505589B true ZA200505589B (en) | 2007-04-25 |
Family
ID=32770265
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ZA200505589A ZA200505589B (en) | 2003-01-23 | 2004-01-23 | DNA molecules encoding a cotton chitinase and promoter |
Country Status (11)
Country | Link |
---|---|
US (2) | US7098324B2 (zh) |
EP (1) | EP1599588B1 (zh) |
CN (1) | CN100564528C (zh) |
AT (1) | ATE445014T1 (zh) |
AU (1) | AU2004205928B2 (zh) |
BR (1) | BRPI0406573B1 (zh) |
DE (1) | DE602004023487D1 (zh) |
ES (1) | ES2332456T3 (zh) |
MX (1) | MXPA05007830A (zh) |
WO (1) | WO2004065571A2 (zh) |
ZA (1) | ZA200505589B (zh) |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7098324B2 (en) * | 2001-07-30 | 2006-08-29 | Texas Tech University | Chitinase encoding DNA molecules from cotton expressed preferentially in secondary walled cells during secondary wall deposition and a corresponding promoter |
US7632654B2 (en) * | 2002-04-29 | 2009-12-15 | Academisch Ziekenhuis Bij De Universiteit Van Amsterdam | Means and methods for detecting endoglycosidase activity |
WO2005011366A1 (en) * | 2003-08-01 | 2005-02-10 | Fonterra Co-Operative Group Limited | Polynucleotides, polypeptides and their use in the production of plants with altered condensed tannins |
US9756798B2 (en) | 2004-11-19 | 2017-09-12 | Patti D. Rubin | Burrow filling compressed growing medium |
US20060107589A1 (en) | 2004-11-19 | 2006-05-25 | Rubin Patti D | Compressed growing medium |
AU2008233406B2 (en) * | 2007-04-03 | 2013-05-30 | Insight Genomics Limited | Control of plant gene expression |
WO2009058869A1 (en) | 2007-10-29 | 2009-05-07 | Oms Investments, Inc. | Compressed coconut coir pith granules and methods for the production and use thereof |
CN101230348B (zh) * | 2007-11-19 | 2010-06-23 | 安徽省农业科学院水稻研究所 | 水稻非胚乳组织表达启动子(OsTSPⅠ)及其应用 |
WO2009146015A2 (en) * | 2008-03-31 | 2009-12-03 | Ceres, Inc. | Promoter, promoter control elements, and combinations, and uses thereof |
CA3020943A1 (en) * | 2009-04-22 | 2010-10-28 | Basf Plant Science Company Gmbh | Whole seed specific promoter |
MX2011013224A (es) * | 2009-06-09 | 2012-06-01 | Pioneer Hi Bred Int | Promotor de endospermo temprano y metodos de uso. |
KR101866904B1 (ko) | 2011-06-23 | 2018-06-14 | 방글라데시 주트 리서치 인스티튜트 | 황마에서 질환 저항성을 부여하는 효소들을 인코딩하는 핵산 분자들 |
US8992653B2 (en) | 2011-09-08 | 2015-03-31 | Novozymes Bioag A/S | Seed treatment methods and compositions |
RU2016124247A (ru) | 2011-09-23 | 2018-11-30 | Новозимс Биоаг А/С | Хитоолигосахариды и способы их применения для усиления роста кукурузы |
MX351416B (es) | 2011-09-23 | 2017-10-13 | Novozymes Bioag As | Quitooligosacaridos y metodos para el uso en el mejoramiento de crecimiento de plantas. |
US8946119B2 (en) | 2011-09-23 | 2015-02-03 | Novozymes Bioag A/S | Chitooligosaccharides and methods for use in enhancing soybean growth |
CA2849889C (en) | 2011-09-23 | 2020-01-07 | Novozymes Biologicals, Inc. | Combinations of lipo-chitooligosaccharides and methods for use in enhancing plant growth |
CN102787105B (zh) * | 2012-07-16 | 2013-11-27 | 河北农业大学 | 棉花尿苷二磷酸葡萄糖脱氢酶基因ugd6、其编码蛋白及应用 |
WO2014198885A1 (en) * | 2013-06-14 | 2014-12-18 | Bayer Cropscience Nv | Plant fibers with improved dyeing properties |
CN105543229B (zh) * | 2016-02-26 | 2018-06-19 | 武汉大学 | 水稻几丁质激发子结合基因启动子及其应用 |
CN106399358A (zh) * | 2016-06-03 | 2017-02-15 | 华南农业大学 | 莲纤维素合酶基因NnuCESA4的应用 |
CN109554379B (zh) * | 2018-12-30 | 2022-02-22 | 中国热带农业科学院热带生物技术研究所 | 一种甘蔗己糖激酶ShHXK8基因及其克隆方法和应用 |
US20220220495A1 (en) | 2019-05-10 | 2022-07-14 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
AU2020344905A1 (en) | 2019-09-12 | 2022-04-28 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
WO2021069387A1 (en) | 2019-10-07 | 2021-04-15 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
EP4069852A1 (en) | 2019-12-03 | 2022-10-12 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
CN110760528B (zh) * | 2019-12-14 | 2022-02-01 | 厦门梓蔓生物科技有限公司 | 一种大麻腺毛中分离的转录因子CsWRKY1及其应用 |
CN111041030B (zh) * | 2019-12-31 | 2021-09-10 | 海南波莲水稻基因科技有限公司 | 一种在水稻花粉中特异表达的启动子pchf4及其应用 |
CN111876429B (zh) * | 2020-04-10 | 2022-05-17 | 中国科学技术大学 | 一种水稻蛋白OsYAF9a在调控水稻株型中的用途 |
AU2021370966A1 (en) * | 2020-10-26 | 2023-06-08 | Galy Co. | Cell lines, varieties, and methods for in vitro cotton fiber production |
CN114350675B (zh) * | 2022-01-11 | 2024-01-26 | 黑龙江省农业科学院经济作物研究所 | 一种调控亚麻次生壁合成的LuNAC基因及其应用 |
CN115725549A (zh) * | 2022-08-10 | 2023-03-03 | 中国海洋大学 | 几丁质酶突变体 |
WO2024083579A1 (en) | 2022-10-20 | 2024-04-25 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5004863B2 (en) | 1986-12-03 | 2000-10-17 | Agracetus | Genetic engineering of cotton plants and lines |
US5495070A (en) | 1988-10-04 | 1996-02-27 | Agracetus, Inc. | Genetically engineering cotton plants for altered fiber |
US5597718A (en) | 1988-10-04 | 1997-01-28 | Agracetus | Genetically engineering cotton plants for altered fiber |
US6329570B1 (en) | 1989-07-19 | 2001-12-11 | Calgene, Llc | Cotton modification using ovary-tissue transcriptional factors |
US5474925A (en) | 1991-12-19 | 1995-12-12 | Agracetus, Inc. | Immobilized proteins in cotton fiber |
US6096950A (en) | 1992-05-18 | 2000-08-01 | Monsanto Company | Cotton fiber-specific promoters |
US6211430B1 (en) | 1995-06-06 | 2001-04-03 | Monsanto Company | FbLate promoter |
US5530187A (en) | 1993-07-16 | 1996-06-25 | The Salk Institute For Biological Studies | Transgenic plants containing multiple disease resistance genes |
US5880110A (en) | 1995-02-21 | 1999-03-09 | Toyobo Co., Ltd. | Production of cotton fibers with improved fiber characteristics by treatment with brassinosteroids |
JPH1094392A (ja) | 1996-09-20 | 1998-04-14 | Nisshinbo Ind Inc | ワタ遺伝子 |
US6259003B1 (en) | 1997-01-21 | 2001-07-10 | Toyo Boseki Kabushiki Kaisha | Cotton plant promoters |
US20020170088A1 (en) * | 2000-11-03 | 2002-11-14 | The Regents Of The University Of California | Novel auxin binding proteins and uses thereof |
US7098324B2 (en) * | 2001-07-30 | 2006-08-29 | Texas Tech University | Chitinase encoding DNA molecules from cotton expressed preferentially in secondary walled cells during secondary wall deposition and a corresponding promoter |
US20030106097A1 (en) * | 2001-07-30 | 2003-06-05 | Haigler Candace H. | Chitinase encoding DNA molecule from cotton expressed preferentially in fibers during secondary cell wall deposition and the corresponding promoter |
-
2003
- 2003-01-23 US US10/350,696 patent/US7098324B2/en not_active Expired - Lifetime
-
2004
- 2004-01-23 AU AU2004205928A patent/AU2004205928B2/en not_active Ceased
- 2004-01-23 MX MXPA05007830A patent/MXPA05007830A/es active IP Right Grant
- 2004-01-23 AT AT04704863T patent/ATE445014T1/de not_active IP Right Cessation
- 2004-01-23 CN CNB2004800071321A patent/CN100564528C/zh not_active Expired - Fee Related
- 2004-01-23 DE DE602004023487T patent/DE602004023487D1/de not_active Expired - Fee Related
- 2004-01-23 BR BRPI0406573A patent/BRPI0406573B1/pt not_active IP Right Cessation
- 2004-01-23 ZA ZA200505589A patent/ZA200505589B/en unknown
- 2004-01-23 WO PCT/US2004/001816 patent/WO2004065571A2/en active Application Filing
- 2004-01-23 EP EP04704863A patent/EP1599588B1/en not_active Expired - Lifetime
- 2004-01-23 ES ES04704863T patent/ES2332456T3/es not_active Expired - Lifetime
-
2006
- 2006-04-04 US US11/397,479 patent/US7674956B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ATE445014T1 (de) | 2009-10-15 |
CN100564528C (zh) | 2009-12-02 |
WO2004065571A3 (en) | 2005-06-09 |
US7674956B2 (en) | 2010-03-09 |
EP1599588B1 (en) | 2009-10-07 |
US7098324B2 (en) | 2006-08-29 |
US20060174379A1 (en) | 2006-08-03 |
WO2004065571A2 (en) | 2004-08-05 |
EP1599588A2 (en) | 2005-11-30 |
AU2004205928B2 (en) | 2007-11-22 |
CN1761751A (zh) | 2006-04-19 |
EP1599588A4 (en) | 2007-05-30 |
BRPI0406573B1 (pt) | 2016-04-05 |
ES2332456T3 (es) | 2010-02-05 |
AU2004205928A1 (en) | 2004-08-05 |
DE602004023487D1 (de) | 2009-11-19 |
US20040049808A1 (en) | 2004-03-11 |
BRPI0406573A (pt) | 2005-12-20 |
MXPA05007830A (es) | 2006-04-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7674956B2 (en) | Chitinase encoding DNA molecules from cotton expressed preferentially in secondary walled cells during secondary wall deposition and a corresponding promoter | |
EP1539784B1 (en) | Chitinase encoding dna molecule from cotton expressed preferentially in fibers during secondary cell wall deposition and the corresponding promoter | |
US20210254115A1 (en) | Plants expressing cell wall degrading enzymes and expression vectors | |
US5777200A (en) | Chemically regulatable and anti-pathogenic DNA sequences and uses thereof | |
US5789214A (en) | Method of inducing gene transcription in a plant | |
Takakura et al. | Flower-predominant expression of a gene encoding a novel class I chitinase in rice (Oryza sativa L.) | |
US9057073B2 (en) | Differential expression of subgenome specific alleles in cotton and uses thereof | |
EP0332104A2 (en) | Chemically regulatable DNA sequences and genes and uses thereof | |
Shani et al. | Expression of endo-1, 4-β-glucanase (cel 1) in arabidopsis thaliana is associated with plant growth, xylem development and cell wall thickening | |
Nardi et al. | Overexpression of the carbohydrate binding module of strawberry expansin2 in Arabidopsis thaliana modifies plant growth and cell wall metabolism | |
WO1990007001A1 (en) | Overexpression of chitinase in transgenic plants | |
Harikrishna et al. | An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes | |
Kellmann et al. | Characterization of two class II chitinase genes from peanut and expression studies in transgenic tobacco plants | |
BG62701B1 (bg) | Хитиназа,кодираща хитиназата днк,и растения,които съдържат такава днк | |
WO1995005467A9 (en) | Chitinase, dna coding therefor and plants containing same | |
JP3538428B2 (ja) | 植物プロモーターおよび該プロモーターを用いた遺伝子発現方法 | |
US20160160254A1 (en) | Plants expressing cell wall degrading enzymes and expression vectors | |
CN111454341A (zh) | 一种促进植物花器官增大的基因及其应用 | |
ZA200501688B (en) | Chitinase encoding DNA molecule from cotton expressed preferentially in fibers during secondary cell wall deposition and the corresponding promoter | |
EP1953231A1 (en) | Compositions and methods for modifying gene expression using the promoter of ubiquitin conjugating protein coding gene of cotton plants | |
WO2001046387A1 (en) | Effect on endochitinase and chitobiosidase and their encoding genes on plant growth and development | |
Olawolea et al. | Promiscuous, non-catalytic, tandem carbohydrate-binding modules alter development of tobacco plants | |
BHJ et al. | The N. tabacum PELPs are Translocated into the Pollen Tube Walls in Inter-Specific Crosses | |
MX2014012167A (es) | Composiciones y metodos para modificar la expresion de genes de interes. |