WO2025232134A1 - 一种水稻突变基因及其用途 - Google Patents

一种水稻突变基因及其用途

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WO2025232134A1
WO2025232134A1 PCT/CN2024/132158 CN2024132158W WO2025232134A1 WO 2025232134 A1 WO2025232134 A1 WO 2025232134A1 CN 2024132158 W CN2024132158 W CN 2024132158W WO 2025232134 A1 WO2025232134 A1 WO 2025232134A1
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rice
mutant gene
xjha
plant height
seq
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French (fr)
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欧阳鑫昊
黄荣裕
郑景生
江良荣
余烽
林佳佳
陈智明
钱崇祯
邱蕾蕾
韩久盼
黄育民
王侯聪
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Xiamen University
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Xiamen University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Definitions

  • This invention relates to a rice mutant gene and its uses.
  • Rice is one of the world's most important food crops, and plant height is a key agronomic trait affecting rice yield, lodging resistance, and fertilizer tolerance (Liu et al. 2018).
  • the genetic regulation mechanism of rice plant height and its application in breeding have always been one of the hot and important issues in rice genetics and breeding research.
  • the application of rice plant height in breeding mainly lies in the discovery and utilization of dwarfing genes.
  • Dwarfing genes can reduce rice plant height, improve lodging resistance, increase stem strength, and reduce nutrient consumption by the stem, thereby increasing yield.
  • sd1 can reduce rice plant height, improve lodging resistance, increase stem strength, and reduce nutrient consumption by the stem, thereby increasing yield.
  • There are multiple alleles of sd1 in rice among which the loss-of-function alleles sd1-d, sd1-AJNT, and sd1-9311 are widely used in indica rice, such as in varieties like IR8, IR36, IR64, and 9311.
  • sd1-r and sd1-j are mainly retained, such as in the "Dongnong" and "Xiushui” series.
  • the main technical problem to be solved by the present invention is to provide a rice mutant gene and its use in regulating rice plant height.
  • Another technical problem to be solved by the present invention is to provide a rice plant height-related mutant gene xjha and its use in regulating rice plant height.
  • Another technical problem to be solved by the present invention is to provide a plant expression vector.
  • Another technical problem to be solved by the present invention is to provide a recombinant bacterium.
  • the present invention provides a rice mutant gene having at least one allelic mutation, wherein the allelic mutation is a mutation of Asp at position 377 in the amino acid sequence shown in SEQ ID NO.26 to Asn.
  • nucleotide sequence of its genome is shown in SEQ ID NO. 01.
  • nucleotide sequence of its cDNA is shown in SEQ ID NO. 02.
  • the allelic mutation is formed by at least one of natural mutation, artificial mutagenesis, and gene editing.
  • the present invention also provides the use of the above-mentioned rice mutant gene in regulating rice plant height.
  • the present invention also provides a rice plant height-related mutant gene xjha, the amino acid sequence of the protein encoded by which is shown in SEQ ID NO.03.
  • nucleotide sequence of its genome is shown in SEQ ID NO. 01.
  • nucleotide sequence of its cDNA is shown in SEQ ID NO. 02.
  • it is formed by at least one of natural mutation, artificial mutagenesis, and gene editing.
  • the present invention also provides the use of the above-mentioned rice plant height-related mutant gene xjha in regulating rice plant height.
  • the present invention also provides a plant expression vector containing the nucleotide sequence of the above-mentioned rice mutant gene or the nucleotide sequence of the above-mentioned rice plant height-related mutant gene xjha.
  • nucleotide sequence of the rice mutant gene or the rice plant height-related mutant gene xjha is loaded onto pRHE.
  • the present invention also provides a recombinant bacterium containing the above-mentioned plant expression vector.
  • the bacteria is Agrobacterium EHA105.
  • This invention can effectively regulate rice plant height and has good application potential in rice variety plant type breeding.
  • Figure 1 shows the mature plants of wild type (WT) and mutant xjha in Example 1 of the present invention (A), with a scale bar of 10 cm; (B-G) comparison of agronomic traits of WT and xjha, in cm, plant height (B), ear length (C), ear stem length (D), internode length (E), leaf length (F), leaf width (G), * indicates significant difference, ** indicates extremely significant difference, ns indicates no significant difference (*P ⁇ 0.05, **P ⁇ 0.01, ns P>0.05).
  • Figure 2 is a statistical chart of the correlation traits of WT and xjha yield in Example 1 (A-E) of the present invention, wherein grain type (A), grain length (B), grain width (C), number of effective panicles (D), thousand-grain weight (E), number of filled grains per panicle (F), seed setting rate (G), and yield per plant (H).
  • Figure 3 shows the localization map of xjha on the chromosome in Example 2 of this invention, where: (A) xjha is located between RM495 and RM13226 on the long arm of chromosome 2; (B) using large-scale linkage analysis of 1143 F2 single plants, xjha is located in the genomic region between markers RM12965 and RM12979, with a physical distance of 131 kb, and 5 functionally annotated genes.
  • Figure 4 shows the haploid types with a sample number greater than 10 in Example 2 of the present invention, indicating that xjha in Example 2 is a new allele mutation.
  • Figure 5 shows the construction of the complementation vector pRHE-OsCPS1p::OsCPS1 in Example 4 of this invention (A), and the comparison of correlation traits between WT, xjha, and plant height of complementation plants (B-E).
  • Plant type, Bar 7cm (B), OsCPS1 mRNA level (C), plant height (D), internode length (E).
  • xjha is a dwarf mutant, a single-base naturally occurring mutation of the OsCPS1 gene found in the field population of the rice variety Jiahe Xinzhan. Compared with the wild-type Jiahe Xinzhan, this mutant plant exhibits reduced plant height and increased tillering, while the seed setting rate and yield are not significantly different from the wild type ( Figure 1). xjha may have good application potential in breeding to improve the lodging resistance of rice varieties.
  • the rice plant height-related mutant gene xjha was obtained from this mutant. Its full-length genome is 10202 bp, and its cDNA coding region is 2604 bp long, as shown in SEQ ID NO.01 and SEQ ID NO.02, respectively.
  • a genetic mapping population was constructed.
  • the dwarf mutant xjha was crossed with the japonica rice variety Samba, and a genetic mapping population was constructed for gene mapping.
  • the gene was located within the interval of 9562527-10806334 on chromosome 2. New molecular markers were developed within this interval, and the interval was continuously narrowed down between two markers, ultimately locating the dwarf gene within a 140kb interval of the marker physical location chr2:10186708-1032662 ( Figure 3).
  • LOC_Os02g17780 can be identified as the target gene for plant height regulation, and this gene is named XJHA (wild type, whose nucleotide sequence is shown in SEQ ID NO.25). It encodes a single expressed protein with pleiotropic effects and is associated with multiple traits of rice plant type ( Figure 3).
  • this embodiment analyzed the haplotypes of this gene using the public database from the 3K Rice Genome Project (https://www.rmbreeding.cn). Fourteen single nucleotide polymorphisms (SNPs) were identified in the OsCPS1 coding region, and rice varieties were divided into 241 haplotypes. Figure 4 shows the haplotypes with more than 10 samples. No SNP sites were found on Exon7 of OsCPS1, indicating that xjha is a novel allele of OsCPS1.
  • cx-CPS1-F catatctcgagttggcgaaacag(SEQ ID NO.10)
  • Example 3 Construction of xjha genetically complementary transgenic plants and identification of transgenic materials
  • a 7821bp gene fragment (including 2166bp before the start codon, the full length of XJHA, and 215bp after the stop codon) from the wild-type XJHA gene xjha shown in SEQ ID NO.01 was amplified by PCR and ligated into the plant expression vector pRHE to construct the genetic complementation vector pRHE-gXJHA ( Figure 4). Then, the genetic complementation vector pRHE-gXJHA was transformed into Agrobacterium EHA105 and then into the semi-dwarf mutant xjha. Specifically, Agrobacterium EHA105 containing the genetic complementation vector pRHE-gXJHA was used to infect xjha mutant callus.
  • the xjha mutant callus was co-cultured at 28°C, sterilized, screened with antibiotics, and differentiated (for about 4 months) to obtain pRHE-gXJHA transgenic plants with the full length of the rice XJHA gene.
  • T0 generation transgenic plants were identified by amplification using the following primers, resulting in T0 generation plants successfully transferred into the genetic complementation vector carrying the target gene.
  • OsCPS1WT can compensate for the plant height defect in xjha indicates that OsCPS1 is the controlling gene for the plant height phenotype of the mutant xjha.
  • XJHA can regulate plant height, indicating that the rice plant height-related gene XJHA in this invention has good application potential in rice variety plant type breeding.
  • the primers used for the above qPCR are as follows:
  • grasses also possess homologous genes to the rice OsCPS1 gene in Example 1.
  • these homologous genes undergo mutations identical or similar to the xjha mutation in the OsCPS1 gene, they can also achieve the same or similar technical effects as in the above examples, namely, partial dwarfing of the plant.
  • These homologous genes include, but are not limited to, the wheat TaCPS3 gene (gene ID: TraesCS7D02G539200) and maize An1 (gene ID: Zm00001d032961/Zm00001d032961).
  • This invention discloses a rice mutant gene and its use in regulating rice plant height.
  • the gene has at least one allelic mutation, specifically a mutation of Asp to Asn at position 377 of the amino acid sequence shown in SEQ ID NO. 26.
  • This invention can effectively regulate rice plant height and has good application potential in rice variety breeding.

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Abstract

提供了一种水稻突变基因及其在调节水稻株高中的用途，其至少具有一等位基因突变，该等位基因突变为如SEQ ID NO.26所示的氨基酸序列中的377位的Asp突变为Asn。能够有效调控水稻株高，在水稻品种株型育种上具有良好的应用潜力。

Description

一种水稻突变基因及其用途 技术领域
本发明涉及一种水稻突变基因及其用途。
 背景技术
水稻是世界上最重要的粮食作物之一，株高是影响水稻产量、抗倒伏和耐肥性的关键农艺性状(Liu et al.2018)。水稻株高的遗传调控机制及其在育种上的应用一直以来是水稻遗传学和育种学研究的热点和重点问题之一。
水稻株高在育种上的应用主要体现在矮秆基因的发掘和利用上。矮秆基因可以降低水稻株高，提高抗倒伏能力，增加茎秆强度，减少茎秆对养分的消耗，从而提高产量。目前与矮秆性状相关的水稻突变体和基因有很多，在水稻各个染色体上均有分布，但已鉴定出的这些半矮秆和矮秆基因大多对水稻的农艺性状表现负面的影响，从而限制了它们在育种中的应用。因此目前应用于育种上的矮秆基因不多，其中应用最广范的就是绿色革命基因sd1。
sd1可以降低水稻株高，提高抗倒伏能力，增加茎秆强度，减少茎秆对养分的消耗，从而提高产量。sd1在水稻中有多个等位基因，其中功能缺失型的sd1-d、sd1-AJNT和sd1-9311在籼稻中得到广泛应用，如IR8、IR36、IR64、9311等品种。粳稻中则主要保留了sd1-r和sd1-j，如“东农”和“秀水”系列。此外，还有通过将sd1-d导入水稻品牌稻花香2号(DHX2)中，并获得了携带sd1-d的矮杆和半矮杆株系(1279和1280)(Sha et al.2022)。然而，过度使用sd1会造成育种品种矮秆基因单一，遗传多样性降低。因此亟需新的可用于育种的水稻半矮秆基因。
综上所述，SD1的广泛应用，但存在着遗传资源单一的脆弱性，因此挖掘和鉴定新的控制株高相关的基因，并揭示其生物功能，对于水稻株高的定向改良以及保证水稻半矮化遗传资源的多样性，具有重大的应用价值。
 发明内容
本发明所要解决的主要技术问题是提供一种水稻突变基因及其在调节水稻株高中的用途。
本发明所要解决的另一技术问题在于提供一种水稻株高相关突变基因xjha及其在调节水稻株高中的用途。
本发明所要解决的再一技术问题在于提供一种植物表达载体。
本发明所要解决的又一技术问题在于提供一种重组菌。
[根据细则91更正 21.02.2025]
为了解决上述的技术问题，本发明提供了一种水稻突变基因，其至少具有一等位基因突变，该等位基因突变为如SEQ ID NO.26所示的氨基酸序列中的377位的Asp突变为Asn。
在一较佳实施例中：其基因组的核苷酸序列如SEQ ID NO.01所示。
在一较佳实施例中：其cDNA的核苷酸序列如SEQ ID NO.02所示。
在一较佳实施例中：形成所述等位基因突变的方式包括自然突变、人工诱变和基因编辑中的至少一种。
本发明还提供了上述水稻突变基因在调节水稻株高中的用途。
本发明还提供了一种水稻株高相关突变基因xjha，其所编码的蛋白质的氨基酸序列如SEQ ID NO.03所示。
在一较佳实施例中：其基因组的核苷酸序列如SEQ ID NO.01所示。
在一较佳实施例中：其cDNA的核苷酸序列如SEQ ID NO.02所示。
在一较佳实施例中：其形成的方式包括自然突变、人工诱变和基因编辑中的至少一种。
本发明还提供了上述水稻株高相关突变基因xjha在调节水稻株高中的用途。
本发明还提供了一种植物表达载体，含有上述水稻突变基因的核苷酸序列或上述水稻株高相关突变基因xjha的核苷酸序列。
在一较佳实施例中：其以pRHE负载所述水稻突变基因的核苷酸序列或所述水稻株高相关突变基因xjha。
本发明还提供了一种重组菌，含有上述植物表达载体。
在一较佳实施例中：其为农杆菌EHA105。
相较于现有技术，本发明的技术方案具备以下有益效果：
本发明能够有效调控水稻株高，在水稻品种株型育种上具有良好的应用潜力。
 附图说明
图1为本发明实施例1(A)野生型(WT)与突变体xjha成熟期植株，标尺为10cm；(B-G)WT与xjha农艺性状比较，单位为cm，株高(B)，穗长(C)，穗茎长(D)，节间长度(E)，叶长(F)，叶宽(G)，*表示显著差异，**表示极显著差异，ns表示无显著差异(*P<0.05,**P<0.01,ns P>0.05)。
图2为本发明实施例1(A-E)WT和xjha产量相关性状统计图，其中，粒型(A)，粒长(B)，粒宽(C)，有效穗数(D)，千粒重(E)，每穗实粒数(F)，结实率(G)，单株产量(H)。
图3为本发明实施例2中xjha染色体上的定位图，其中：((A))将xjha定位于2号染色体长臂RM495和RM13226之间，((B))利用1143个F2单株进行大规模连锁分析，将xjha定位于标记RM12965和RM12979之间的基因组区域,其物理距离为131Kb，有功能注释的基因有5个。对WT和xjha的OsCPS1基因进行测序，发现与WT相比，xjha的OsCPS1其第7号外显子上第48位的碱基由G突变为A，使得第377个氨基酸由天冬氨酸Asp(D)变成天冬酰胺Asn(N)导致的(OsCPS1D377N)。蓝色方块代表外显子，灰色线条代表内含子，红色方块代表第7外显子，橘色方块代表5’UTR和3’UTR。
图4展示了本发明实施例2中的样品数大于10的单倍体类型，表明实施例2中的xjha为新的等位基因突变。
图5为本发明实施例4中(A)回补载体pRHE-OsCPS1p::OsCPS1构建，(B-E)WT、xjha和回补植株株高相关性状比较。株型，Bar＝7cm(B)，OsCPS1 mRNA水平(C)，株高(D)，节间长度(E)。
具体连接方式
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述；显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例，基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
实施例1突变体与野生型株型比较
本实施例中的xjha为一个矮秆突变体，是在水稻品种佳禾新占田间群体中发现一个OsCPS1基因单碱基自然突变的水稻单株，与野生型佳禾新占相比，该突变单株表现为株高降低、分蘖增加，而结实率、产量与野生型没有显著差异(图1)，xjha在提高水稻品种抗倒性育种上可能具有良好的应用潜力。从该突变株获得了水稻株高相关突变基因xjha，其基因组全长10202bp，其cDNA编码区序列长2604bp，分别如SEQ ID NO.01和SEQ ID NO.02所示，其编码867个氨基酸，如SEQ ID NO.03所示，且该突变为等位基因矮化突变。而相对于该突变的野生型的水稻株高相关基因XJHA所编码的蛋白质的氨基酸序列如SEQ ID NO.26所示。
实施例2 xjha基因图位克隆
为了定位克隆实施例1中的矮秆突变体株高的调控基因，构建了遗传定位群体。实施例将矮秆突变体xjha与偏粳型水稻品种Samba杂交，构建遗传定位群体用于基因定位。经过选取突变体xjha与偏粳型品种Samba杂交F2群体中20株突变表型非常显著的单株进行混池筛选和单株验证，将该基因定于第2条染色体物理位置为9562527-10806334的区间之内。此区间内开发了新的分子标记，在两个标记中间不断缩小区间，最终将该矮秆基因定位于标记物理位置为chr2:10186708-1032662的140kb区间内(图3)。
经过进一步的测序分析，本实施例发现xjha在基因LOC_Os02g17780第7号外显子上第48位的碱基由G突变为A，使得第377个氨基酸Asp变成Asn，进而影响XJHA蛋白的功能，因此可以确定LOC_Os02g17780(OsCPS1)为株高调控基因的目的基因，并将该基因命名XJHA(野生型，其基因组的核苷酸序列如SEQ ID NO.25所示)，其编码一个表达蛋白，具有多效性，与水稻株型的多个性状相关(图3)。
此外，为了表征OsCPS1的遗传多样性，本实施例利用3K水稻基因组计划中的公共数据库(https://www.rmbreeding.cn)分析了该基因的单倍型。在OsCPS1编码区中鉴定了14个单核苷酸多态性(SNPs)，并将水稻品种分成了241个单倍型(Haps)。图4展示了样品数大于10的单倍体类型，未在OsCPS1的Exon7上发现SNP位点，说明xjha是OsCPS1的一个新的等位基因。
本实施例所用到的测序引物如下：
CPS1-CDS1/2-F：gcatcgcatctccatcatctcc(SEQ ID NO.04)
CPS1-CDS1/2-R：cctactgttcttcgatcgggttc(SEQ ID NO.05)
CPS1-CDS3/4-F：aactataccaaaaacatgcggcac(SEQ ID NO.06)
CPS1-CDS3/4-R：tgtaacaaacctatctcaaactgtctatc(SEQ ID NO.07)
CPS1-CDS5/6-F：gctaagttgctataatttaagacggagg(SEQ ID NO.08)
CPS1-CDS5/6-R：caacatcacatgatctgagatcagc(SEQ ID NO.09)
cx-CPS1-F：catatctcgagttggcgaaacag(SEQ ID NO.10)
CPS1-CDS7/8/9-F：gattcgtggcatttcactgacc(SEQ ID NO.11)
CPS1-CDS7/8/9-R：cagtgcaatgttatatcagtactcagg(SEQ ID NO.12)
CPS1-CDS10/11/12-F：cctaccaggagaggtataatctagttac(SEQ ID NO.13)
CPS1-CDS10/11/12-R：catagagaggacaagcatcagaag(SEQ ID NO.14)
CPS1-CDS13/14-F：gagaagaatgcttgccttggagtac(SEQ ID NO.15)
CPS1-CDS13/14-R：caattcagtgaatacagcttgtcaag(SEQ ID NO.16)
CPS1-CDS15-F：gataattctaactctcaaggcatctcc(SEQ ID NO.17)
CPS1-CDS15-R：cacatgtagaaccatacagagatacatc(SEQ ID NO.18)
实施例3：构建xjha遗传互补转基因植株以及转基因材料的鉴定
本实施例将相对于SEQ ID NO.01所示的基因xjha的野生型XJHA中的7821bp的基因片段(包含XJHA起始密码子前2166bp、XJHA全长及终止密码子后215bp)进行PCR扩增并将其连接至植物表达载体pRHE，构建遗传互补载体pRHE-gXJHA(图4)，进而将遗传互补载体pRHE-gXJHA转入农杆菌EHA105中转化到半矮秆突变体xjha中，具体为：将含有遗传互补载体pRHE-gXJHA的农杆菌EHA105侵染xjha突变体愈伤，在28℃进行共培养、除菌、抗生素筛选和分化(大约4个月的时间)，获得水稻XJHA基因全长的pRHE-gXJHA转基因植株。
T0代转基因植株鉴定：利用以下引物进行扩增，鉴定到负载目的基因的上述遗传互补载体成功转入的T0代植株。
gXJHA-F：gggagcaaacaccgcaaagg(SEQ ID NO.19)
gXJHA-R：agaacaaatccaaaataaataatttataaaaaatg(SEQ ID NO.20)
NosR-seq：agaccggcaacaggattcaatc(SEQ ID NO.21)
CPS1-CDS15-F：gataattctaactctcaaggcatctcc(SEQ ID NO.22)
遗传互补转基因植株平均株高(82.4cm)比突变体xjha(62.0cm)增加32.9％，且与野生型WT(91.0cm)相比无显著性差异(图5)。通过qPCR检测回补材料中OsCPS1的mRNA表达水平发现第六株回补材料(cp6)不仅株高得到回补且mRNA水平并未发生明显变化(图5)。测量cp6的节间长度发现，cp6的各节间长度特别是倒一节间和倒二节间比xjha有显著伸长，与WT的节间长度基本一致(图5)。OsCPS1WT能回补xjha株高性状上的缺陷说明OsCPS1是突变体xjha株高表型的控制基因。如图5所示，XJHA能够调控株高，表明本发明中的水稻株高相关基因XJHA在水稻品种株型育种上具有良好的应用潜力。
上述qPCR所用引物如下：
QCPS1-F：gaacgtttacccggtcgatc(SEQ ID NO.23)
Qcps1-R：cttcagtccagtgcctgttg(SEQ ID NO.24)
本领域普通技术人员可知，禾本科植物也存在与实施例1中的水稻OsCPS1基因的同源基因，当这些同源基因发生与OsCPS1基因的xjha突变相同或相似的突变，也能够获得与上述实施例相同或相似的技术效果，即植株发生部分矮化。以上同源基因包括但不限于小麦TaCPS3基因(基因ID:TraesCS7D02G539200)和玉米An1(基因ID:Zm00001d032961/Zm00001d032961)。
以上所述，仅为本发明较佳的具体实施方式，但本发明的设计构思并不局限于此，任何熟悉本技术领域的技术人员在本发明揭露的技术范围内，利用此构思对本发明进行非实质性的改动，均属于侵犯本发明保护范围的行为。
 工业实用性
[根据细则91更正 21.02.2025]
本发明公开了一种水稻突变基因及其在调节水稻株高中的用途，其至少具有一等位基因突变，该等位基因突变为如SEQ ID NO.26所示的氨基酸序列中的377位的Asp突变为Asn。本发明能够有效调控水稻株高，在水稻品种株型育种上具有良好的应用潜力。

Claims (14)

  1. [根据细则91更正 21.02.2025]
    一种水稻突变基因，其特征在于：其至少具有一等位基因突变，该等位基因突变为如SEQ ID NO.26所示的氨基酸序列中的377位的Asp突变为Asn。
  2. 如权利要求1所述的一种株高相关突变基因，其特征在于：其基因组的核苷酸序列如SEQ ID NO.01所示。
  3. 如权利要求2所述的一种水稻突变基因，其特征在于：其cDNA的核苷酸序列如SEQ ID NO.02所示。
  4. 如权利要求1至3中任一项所述的一种水稻突变基因，其特征在于：形成所述等位基因突变的方式包括自然突变、人工诱变和基因编辑中的至少一种。
  5. 权利要求1至4中任一项所述的水稻突变基因在调节水稻株高中的用途。
  6. 一种水稻株高相关突变基因xjha，其特征在于：其所编码的蛋白质的氨基酸序列如SEQ ID NO.03所示。
  7. 如权利要求6所述的一种水稻株高相关突变基因xjha，其特征在于：其基因组的核苷酸序列如SEQ ID NO.01所示。
  8. 如权利要求7所述的一种水稻株高相关突变基因xjha，其特征在于：其cDNA的核苷酸序列如SEQ ID NO.02所示。
  9. 如权利要求6至8中任一项所述的一种水稻株高相关突变基因xjha，其特征在于：其形成的方式包括自然突变、人工诱变和基因编辑中的至少一种。
  10. 权利要求6至9中任一项所述的水稻株高相关突变基因xjha在调节水稻株高中的用途。
  11. 一种植物表达载体，其特征在于：含有权利要求1至4中任一项所述的水稻突变基因的核苷酸序列或权利要求6至9中任一项所述的一种水稻株高相关突变基因xjha的核苷酸序列。
  12. 如权利要求11所述的一种植物表达载体，其特征在于：其以pRHE负载所述水稻突变基因的核苷酸序列或所述水稻株高相关突变基因xjha。
  13. 一种重组菌，其特征在于：含有权利要求11或12所述的植物表达载体。
  14. 如权利要求13所述的一种重组菌，其特征在于：其为农杆菌EHA105。
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