WO2023026366A1 - 電気泳動支援方法 - Google Patents

電気泳動支援方法 Download PDF

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Publication number
WO2023026366A1
WO2023026366A1 PCT/JP2021/031017 JP2021031017W WO2023026366A1 WO 2023026366 A1 WO2023026366 A1 WO 2023026366A1 JP 2021031017 W JP2021031017 W JP 2021031017W WO 2023026366 A1 WO2023026366 A1 WO 2023026366A1
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WO
WIPO (PCT)
Prior art keywords
electrophoresis
capillary
container
sample
value
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2021/031017
Other languages
English (en)
French (fr)
Japanese (ja)
Inventor
宏一 加藤
隆介 木村
周志 隅田
功 原浦
基博 山崎
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi High Tech Corp
Original Assignee
Hitachi High Tech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi High Tech Corp filed Critical Hitachi High Tech Corp
Priority to JP2023543525A priority Critical patent/JP7585504B2/ja
Priority to US18/578,036 priority patent/US20240337622A1/en
Priority to PCT/JP2021/031017 priority patent/WO2023026366A1/ja
Priority to CN202180100492.XA priority patent/CN117642628A/zh
Publication of WO2023026366A1 publication Critical patent/WO2023026366A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44713—Particularly adapted electric power supply
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means

Definitions

  • FIG. 1 is a schematic perspective view of a capillary electrophoresis device 1 according to this embodiment.
  • the X axis corresponds to the width direction of the capillary electrophoresis device 1
  • the Y axis corresponds to the depth direction of the capillary electrophoresis device 1
  • the Z axis corresponds to the height direction of the capillary electrophoresis device 1.
  • the upward direction is the direction from the autosampler unit 100 to the irradiation detection/bath unit 200, and the downward direction is the opposite direction. Incidentally, the upward direction is also the direction of the Z-axis.
  • the tip of the capillary 311 protrudes from the hollow electrode 312 by about 0.5 mm.
  • the protruding length of the hollow electrode 312 at the tip of the capillary 311 is not limited to 0.5 mm.
  • all of the hollow electrodes 312 provided in each capillary 311 are attached to the load header 301 (see FIG. 3A) in an integrated state. All the hollow electrodes 312 are connected to the high voltage power source 402 via the load header 301 . Since the high-voltage power supply 402 applies a negative voltage to the hollow electrode 312, the hollow electrode 312 becomes a cathode electrode when a voltage is applied to the hollow electrode 312, such as during electrophoresis or sample introduction.
  • the measurement by the first ammeter 401 measures the current through the capillary 311, the hollow electrode 312, and the like. That is, when an electric leakage occurs in the capillary 311 or the like, the current value obtained by the first ammeter 401 is affected.
  • the second ammeter 403 measures the current between the GND 411 and the electrode 221 . This measured value (current value: measured value based on the current flowing through the capillary 311) is not affected by electrical leakage in the capillary 311 or the like.
  • the potential of the electrode 221 immediately becomes the same potential as the GND 411, and the second ammeter The current flowing through 403 becomes zero.
  • the current value of the first ammeter 401 also becomes 0 as the energizing path is interrupted, it is desirable to use the current value of the second ammeter 403 in consideration of the influence of the above-described electric leakage. Therefore, the first ammeter 401 can be omitted. However, it is also possible to use the first ammeter 401 to check the energization (described later) in this embodiment.
  • the reason for this is that it is not confirmed in advance (before step S121) whether there is an abnormality in the energization of the anode-side electrophoresis buffer container 132 and the cathode-side electrophoresis buffer container 143 .
  • an error is detected after the migration medium 120a is transferred to the capillary array 300, so the migration medium 120a is wasted.
  • the electrophoresis medium 120a is the most expensive reagent for electrophoresis and increases running costs.
  • the cause of the error can be identified as originating from the migration medium 120a. Identifying and correcting water level issues allows the run to continue quickly.
  • waste of the electrophoresis medium 120a which is the most expensive reagent, can be prevented.
  • the electrophoresis medium 120a is expensive.
  • the energization state between the lateral electrophoresis buffer solution container 143 is confirmed in advance.
  • the cause of the abnormality is the migration medium 120a.
  • the energization check unit 511 checks the energization state of the energization path at every appropriate timing. This makes it possible to identify the source of the error and take immediate countermeasures. Therefore, the user can correct the error based on the instructions presented on the input/output device 503 without completely aborting the run. In addition, the once interrupted run can be quickly resumed. Also, since the consumables that cause the error can be identified, replacement of consumables unrelated to the error can be avoided. This makes it possible to reduce running costs.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Power Engineering (AREA)
  • Engineering & Computer Science (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
PCT/JP2021/031017 2021-08-24 2021-08-24 電気泳動支援方法 Ceased WO2023026366A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2023543525A JP7585504B2 (ja) 2021-08-24 2021-08-24 電気泳動支援方法
US18/578,036 US20240337622A1 (en) 2021-08-24 2021-08-24 Electrophoresis assistance method
PCT/JP2021/031017 WO2023026366A1 (ja) 2021-08-24 2021-08-24 電気泳動支援方法
CN202180100492.XA CN117642628A (zh) 2021-08-24 2021-08-24 电泳辅助方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2021/031017 WO2023026366A1 (ja) 2021-08-24 2021-08-24 電気泳動支援方法

Publications (1)

Publication Number Publication Date
WO2023026366A1 true WO2023026366A1 (ja) 2023-03-02

Family

ID=85321863

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2021/031017 Ceased WO2023026366A1 (ja) 2021-08-24 2021-08-24 電気泳動支援方法

Country Status (4)

Country Link
US (1) US20240337622A1 (https=)
JP (1) JP7585504B2 (https=)
CN (1) CN117642628A (https=)
WO (1) WO2023026366A1 (https=)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11108889A (ja) * 1997-09-30 1999-04-23 Hitachi Ltd キャピラリー電気泳動装置
JP2003344356A (ja) * 2002-05-31 2003-12-03 Hitachi High-Technologies Corp 電気泳動装置、及び電気泳動方法
JP2007107915A (ja) * 2005-10-11 2007-04-26 Shimadzu Corp キャピラリ流路における電気泳動方法及びマイクロチップ処理装置
JP2017201286A (ja) * 2016-04-27 2017-11-09 パナソニックＩｐマネジメント株式会社 電気泳動媒体によって満たされたキャピラリーが電気泳動のために適切に用いられるかどうかを判定する方法
WO2021166210A1 (ja) * 2020-02-21 2021-08-26 株式会社日立ハイテク 電気泳動装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09281077A (ja) * 1996-04-16 1997-10-31 Hitachi Ltd キャピラリ−電気泳動装置
JP2006119158A (ja) * 2006-01-30 2006-05-11 Hitachi High-Technologies Corp 電気泳動装置、及び電気泳動方法
CN206756744U (zh) * 2017-02-15 2017-12-15 张钰婕 一种具有浓缩功能的电泳仪
US11592421B2 (en) * 2017-05-22 2023-02-28 IntegenX, Inc. Serial electrophoresis
JP6770650B2 (ja) * 2017-07-31 2020-10-14 株式会社日立ハイテク キャピラリ電気泳動装置

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11108889A (ja) * 1997-09-30 1999-04-23 Hitachi Ltd キャピラリー電気泳動装置
JP2003344356A (ja) * 2002-05-31 2003-12-03 Hitachi High-Technologies Corp 電気泳動装置、及び電気泳動方法
JP2007107915A (ja) * 2005-10-11 2007-04-26 Shimadzu Corp キャピラリ流路における電気泳動方法及びマイクロチップ処理装置
JP2017201286A (ja) * 2016-04-27 2017-11-09 パナソニックＩｐマネジメント株式会社 電気泳動媒体によって満たされたキャピラリーが電気泳動のために適切に用いられるかどうかを判定する方法
WO2021166210A1 (ja) * 2020-02-21 2021-08-26 株式会社日立ハイテク 電気泳動装置

Also Published As

Publication number Publication date
JPWO2023026366A1 (https=) 2023-03-02
JP7585504B2 (ja) 2024-11-18
CN117642628A (zh) 2024-03-01
US20240337622A1 (en) 2024-10-10

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