Classifications

• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
  • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
  • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
  • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
  • A01N25/06—Aerosols
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
  • A01N31/02—Acyclic compounds
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
  • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
  • A61L9/00—Disinfection, sterilisation or deodorisation of air
  • A61L9/01—Deodorant compositions
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
  • A61L9/00—Disinfection, sterilisation or deodorisation of air
  • A61L9/14—Disinfection, sterilisation or deodorisation of air using sprayed or atomised substances including air-liquid contact processes
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
  • C11B9/00—Essential oils; Perfumes
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
  • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
  • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
  • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

• the present invention relates to a virus inactivating composition for liquid spray and a virus inactivating method.
• Viral infections are diseases that cause severe symptoms such as cold symptoms, pneumonia, hepatitis, and encephalitis, and are an eternal threat to humankind.
• influenza viruses have raged all over the world, and in some cases, outbreaks of new strains of influenza with altered antigenicity have caused pandemics.
• SARS coronavirus-2 SARS-CoV-2
• Viruses are transmitted by contact infection through fingers and various instruments and parts, droplet infection by directly inhaling droplets generated by coughing, sneezing, and conversation, or airborne infection by inhaling droplet nuclei floating in the air. expands. Therefore, it is considered effective to remove viruses or inactivate viruses by cleaning and disinfecting objects that may adhere to viruses, and to inactivate viruses that are splashed or floating in space to prevent the spread of infection. It is
• Patent Document 1 Japanese Patent Publication No. 4-502616
• the present invention relates to the following 1) to 3).
• a virus inactivating composition for liquid spray A1) A composition having a boiling point of 220° C. or lower and containing 40 to 90% by mass of a diol having 2 to 4 carbon atoms and 10 to 60% by mass of water.
• A1) A composition containing 40 to 90% by mass of a diol having 2 to 4 carbon atoms and 10 to 60% by mass of water having a boiling point of 220°C or less is applied to a hard or soft surface of an inanimate object that is concerned about virus contamination. Or a virus inactivation method by spraying on waste.
• a composition having a boiling point of 220°C or lower and containing 40 to 90% by mass of a diol having 2 to 4 carbon atoms and 10 to 60% by mass of water is sprayed onto the carrier, and A1) in the space has a boiling point of 220°C or lower.
• a method for inactivating viruses in a target space wherein the composition is vaporized from the carrier so that the concentration of the diol having 2 to 4 carbon atoms is 20 ppm or more.
• ethanol, sodium hypochlorite, chlorine dioxide, glutaraldehyde, etc. have been used for the purpose of inactivating viruses.
• these agents are highly irritating to mucous membranes and skin, their applications are limited due to safety concerns and allergy concerns.
• it is not suitable for virus inactivation using an atomizer.
• disinfection by spraying ethanol on work spaces in vehicles such as automobiles (garbage trucks, buses, etc.), trains, aircraft, etc. is limited due to the danger of ignition.
• Spraying chlorine dioxide has also been devised as a method of chemically inactivating viruses in space, but its effectiveness is uncertain.
• a diol having a boiling point of 220° C. or less and having 2 to 4 carbon atoms is a type of polyhydric alcohol and is used in solvents, antifreeze liquids, lubricating oils, pharmaceuticals, and the like.
• US Pat. No. 6,200,000 discloses a virucidal liquid composition containing about 10-20% by weight of glutaraldehyde and a glycol such as propylene glycol to reduce its odor.
• a diol having a boiling point of 220° C. or lower such as propylene glycol and having 2 to 4 carbon atoms has an inactivating effect on influenza viruses and the like.
• the present invention relates to providing a virus inactivation composition for liquid spray and a virus inactivation method that enable inactivation of viruses present in the environment.
• the present inventor has found that a composition with a boiling point of 220° C. or lower, a diol having 2 to 4 carbon atoms and water at specific concentrations can be sprayed, and a composition with a boiling point of 220° C. or lower and a It was found that diols of ⁇ 4 exhibit virus inactivation effects. In addition, alkyl glyceryl ethers and specific fragrances have the effect of inactivating viruses, and it was found that combining these with diols having a boiling point of 220 ° C. or less and having 2 to 4 carbon atoms improves the virus inactivating effect. rice field.
• viruses adhering to objects in the living environment and viruses in the air can be inactivated, and the spread of infection by the virus can be prevented or reduced.
• the virus inactivating composition for liquid spray of the present invention contains A1) a diol having a boiling point of 220° C. or less and having 2 to 4 carbon atoms. Further, the composition of the present invention preferably uses a diol having a boiling point of 220° C. or lower and having 2 to 4 carbon atoms as a virus-inactivating active ingredient.
• the boiling point means the initial boiling point at 1 atm or the temperature range of the boiling state.
• ethylene glycol (boiling point 197° C.), 1,2-propanediol (boiling point 188° C.), 1,3-propanediol (boiling point 213° C.), 1,2-butanediol (boiling point 192° C.), 1,3-butanediol (boiling point 207°C) and 2,3-butanediol (boiling point 177°C) is mentioned.
• 1,2-propanediol (boiling point 188°C), 2,3-butanediol (boiling point 177°C), 1,2-butanediol (boiling point 192°C) and , ethylene glycol (boiling point 197° C.) is preferable, and 1,2-propanediol (boiling point 188° C.) is more preferable from the viewpoint of less odor.
• the composition of the present invention preferably contains A1) a diol having a boiling point of 220° C. or lower and having 2 to 4 carbon atoms, and A2) a glycol ether having a boiling point of 220° C. or lower and having 6 carbon atoms.
• the content of A2) in the composition is 50% by mass or less, and the total amount of A1) and A2) is more than 40% by mass and 90% by mass or less.
• diethylene glycol monoethyl ether also known as ethyl carbitol, ethyl diglycol, boiling point 202° C.
• ethylene glycol monobutyl ether also known as butyl cellosolve, boiling point 171° C.
• diethylene glycol monoethyl ether is preferable from the viewpoint of little odor.
• the content of a glycol ether having a boiling point of 220° C. or lower and having 6 carbon atoms in the composition is from the viewpoint of reducing the viscosity of the composition and making it easier to spray, and when a fragrance is included, it is easy to solubilize the fragrance.
• it is preferably 1% by mass or more, more preferably 3% by mass or more, and even more preferably 7% by mass or more.
• the indicator property of the scent means that the scent is easily recognizable as an indicator of whether or not it has been sprayed, and the disappearance of the scent is easy to understand.
• A1) a diol having a boiling point of 220° C. or less and having 2 to 4 carbon atoms in the composition, and A2) a glycol ether having a boiling point of 220° C. or less and having 6 carbon atoms in the total amount of A2) having a boiling point of 220° C.
• the mass ratio of the glycol ether having 6 carbon atoms [A2) / (A1) + A2))] is from the viewpoint of reducing the viscosity of the composition and making it easier to spray, and when containing a fragrance, it facilitates the solubilization of the fragrance, From the viewpoint of enhancing the scent indicator property and antiviral effect of perfume, it is preferably 0.05 or more, more preferably 0.1 or more, and even more preferably 0.15 or more. In addition, from the viewpoint of reducing damage to the base material of the object to be sprayed, it is preferably 1.0 or less, more preferably 0.5 or less, and even more preferably 0.3 or less.
• composition of the present invention preferably further contains A3) alkyl glyceryl ether from the viewpoints of viscosity reduction and virus inactivation.
• alkyl glyceryl ether an alkyl glyceryl having one alkyl group having 8 to 18 carbon atoms, preferably 8 to 14 carbon atoms, more preferably 8 to 12 carbon atoms, still more preferably 8 to 10 carbon atoms ethers.
• the alkyl group includes a straight-chain alkyl group or a branched-chain alkyl group, preferably a branched-chain alkyl group, more preferably a 2-ethylhexyl group, an isononyl group or an isodecyl group, and still more preferably a 2-ethylhexyl group.
• the alkyl glyceryl ether is preferably an alkyl glyceryl ether having one branched-chain alkyl group having 8 to 12 carbon atoms, more preferably an alkyl glyceryl ether having one branched-chain alkyl group having 8 carbon atoms. More preferred is ethylhexyl glyceryl ether.
• the content of alkyl glyceryl ether in the composition is preferably 0.1% by mass or more, more preferably 0.2% by mass or more, and still more preferably 0% by mass, from the viewpoint of reducing viscosity and virus inactivation. 4% by mass or more, and preferably 2% by mass or less, more preferably 1% by mass or less, and even more preferably 0.9% by mass or less from the viewpoint of reducing irritation when contacting the human body after spraying, Even more preferably, it is 0.8% by mass or less.
• compositions are preferably 0.05 from the viewpoint of reducing the viscosity.
• the composition of the present invention further contains a perfume (excluding the diols having a boiling point of 220° C. or less and having 2 to 4 carbon atoms) as a scent indicator for determining whether or not the composition has been sprayed.
• a fragrance indicator is a fragrance that can be perceived when sprayed, but does not linger for a long time and serves as an indicator of whether or not it has been sprayed on an object.
• the perfume preferably has a boiling point of 120° C. or higher and 260° C. or lower, more preferably 130° C. or higher and 220° C. or lower, and more preferably 140° C. or higher and 220° C. or lower.
• the indicator property of the scent means that the scent is easily recognizable and disappearance is easy to understand.
• perfumes include hydrocarbons, aldehydes, ketones, alcohols, phenols, lactones, esters, ethers, thiols and the like.
• monoterpene hydrocarbons, aldehydes, alcohols, and esters are preferable from the viewpoint that the fragrance quality is preferable and many compounds have a low threshold value.
• These can be used individually or in combination of 2 or more types.
• a compound with a low threshold value is preferred here because the scent can be perceived even in a low amount.
• Suitable monoterpene hydrocarbons include, for example, limonene (boiling point 177°C).
• Limonene can be used in the form of limonene-rich essential oils, such as lemon oil, orange oil, grapefruit oil, bergamot oil, and yuzu oil.
• aldehydes examples include compounds represented by the following general formula (I).
• R 1 represents a hydrocarbon group having 4 to 10 carbon atoms which may have a substituent
• A represents a hydroxy group or any of the following structures (a) or (b) is;
• R 2 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms.
• the hydrocarbon group having 4 to 10 carbon atoms represented by R 1 includes an aliphatic hydrocarbon group and an aromatic hydrocarbon group.
• the aliphatic hydrocarbon group may be linear, branched or cyclic, and saturated or unsaturated.
• the saturated aliphatic hydrocarbon group is a linear, branched or cyclic saturated aliphatic hydrocarbon group such as n-butyl, isobutyl, sec-butyl, tert-butyl, 1- Alkyl groups such as methylbutyl group, 2-methylbutyl group, 3-methylbutyl group, pentyl group, hexyl group, heptyl group, octyl group, nonyl group, decyl group; cyclobutyl group, cyclopentyl group, cyclohexyl group, 2-isopropyl-5- Cycloalkyl groups such as a methylcyclohexyl group (menthyl group), a cycloheptyl group, a cyclooctyl group, a cyclononyl group, and a cyclodecyl group are included.
• the unsaturated aliphatic hydrocarbon group is a linear, branched or cyclic unsaturated aliphatic hydrocarbon group having one or more double bonds in the molecule, such as n-butenyl Alkenyl groups such as group, isobutenyl group, pentenyl group, hexenyl group, heptenyl group, octenyl group, 3,7-dimethyl-6-octenyl group, nonenyl group, decenyl group; cyclobutenyl group, cyclopentenyl group, cyclohexenyl group, 1 -cycloalkenyl groups such as isopropyl-4-methyl-3-cyclohexenyl; butadienyl, pentadienyl, hexadienyl, heptadienyl, octadienyl, nonadienyl, decandienyl, 1,5-dimethyl-1-vinyl-4
• the group that can be substituted for the hydrocarbon group having 4 to 10 carbon atoms preferably includes an alkoxy group having 1 to 3 carbon atoms.
• the alkoxy group having 1 to 3 carbon atoms means a linear or branched alkoxy group having 1 to 3 carbon atoms, and examples thereof include methoxy group, ethoxy group, n-propoxy group and isopropoxy group.
• the alkyl group having 1 to 3 carbon atoms represented by R 2 represents a linear, branched or cyclic alkyl group having 1 to 3 carbon atoms, such as methyl group, ethyl group, n-propyl group, isopropyl group and cyclopropyl group.
• R 1 is an alkyl group or alkenyl group having 4 to 10 carbon atoms
• A is a hydroxy group or one of the above structures (a) or (b);
• R 2 is a hydrogen atom or a methyl group.
• fragrances of the present invention are the following fragrances having a boiling point in the range of 120°C to 260°C.
• fragrances with a boiling point of 120°C to 130°C isoamyl formate (boiling point 123°C), butyl acetate (boiling point 126°C), cis-3-hexenal (boiling point 126°C), and the like.
• fragrances with a boiling point of over 130°C to 140°C 3-methyl-1-butanol (isoamyl alcohol, boiling point 132°C), 1-pentanol (normal amyl alcohol, boiling point 138°C), and the like.
• fragrances with a boiling point of over 140°C to 180°C isoamyl acetate (boiling point 142°C), trans-2-hexenal (boiling point 146°C), cis-3-hexenyl formate (boiling point 155°C), hexyl formate (boiling point 155°C), trans-3-hexenol (boiling point 155°C), cis-3-hexenol (boiling point 156°C), hexyl alcohol (boiling point 156°C), trans-2-hexenol (boiling point 159°C), trimethylhexanal (3,5,5-trimethylhexanal, boiling point 167°C), cis-acetic acid 3-hexenyl (boiling point 169°C), dimethol (registered trademark, 2,4-dimethyl-2-heptanol, boiling point 170°C), hexane
• fragrances with a boiling point of more than 180°C to 220°C or less Melonal (2,6-dimethyl-5-heptenal, boiling point 188°C), Cyclovertal (registered trademark, 3,6-dimethylcyclohexe-3-ene-1-carbaldehyde, boiling point 189°C), trimethylhexanol (3 ,5,5-trimethylhexanol, boiling point 193°C), dihydromyrcenol (boiling point 194°C), linalool (boiling point 199°C), linalyl formate (boiling point 202°C), benzyl alcohol (boiling point 205°C), benzyl acetate (boiling point 212°C), dimethylbenzylcarbinol (2-methyl-1-phenylpropan-2-ol, boiling point 215°C), geranyl formate (boiling point 216°C), phenylethy
• fragrances with a boiling point of more than 220°C to 260°C or less Syringaldehyde (2-(4-(methylphenyl)acetaldehyde) (boiling point 221°C), phenylpropylaldehyde (boiling point 222°C), hydratropic aldehyde (2-phenylpropanal, boiling point 222°C), phenylethyl formate (boiling point 226°C), geraniol (boiling point 230°C), citronellyl formate (boiling point 235°C), mugol (3,7-dimethyl-4,6-octadien-3-ol, boiling point 237°C) methoxycitronellal (boiling point 238°C) , geranyl acetate (boiling point 245°C), cinnamic alcohol (boiling point 250°C), cinnamyl
• perfumes having a boiling point in the range of 120° C. to 220° C. such as isoamyl formate (boiling point 123° C.), butyl acetate (boiling point 126° C.), cis-3-hexenal (boiling point 126° C.), 3-methyl- 1-butanol (isoamyl alcohol, boiling point 132°C), 1-pentanol (normal amyl alcohol, boiling point 138°C), isoamyl acetate (boiling point 142°C), trans-2-hexenal (boiling point 146°C), cis-3-formate Hexenyl (boiling point 155°C), hexyl formate (boiling point 155°C), trans-3-hexenol (boiling point 155°C), cis-3-hexenol (boiling point 156°C), hexyl alcohol (
• the composition of the present invention preferably contains a perfume that not only works as a scent indicator but also has a virus-inactivating effect.
• suitable fragrances are as follows. isoamyl formate (boiling point 123° C.), butyl acetate (boiling point 126° C.), 3-methyl-1-butanol (boiling point 132° C.), 1-pentanol (boiling point 138° C.), isoamyl acetate (boiling point 142° C.), trans-2-hexenal (boiling point 146° C.), cis-3-hexenyl formate (boiling point 155° C.), trans-3-hexenol (boiling point 155° C.), cis-3-hexenol (boiling point 156° C.), hexyl alcohol (boiling point 156° C.), trans-2-hexeno
• the composition of the present invention preferably contains 0.001 to 1.0% by mass, more preferably 0.001 to 1.0% by mass of a fragrance having a boiling point of 120° C. or higher and 260° C. or lower from the standpoints of scent indicator properties and virus inactivation. It is preferably contained in the range of 01 to 0.5% by mass.
• the composition may contain other perfumes (having a boiling point of less than 120° C. or more than 260° C.), but the content thereof is preferably 0.5% by mass or less, more preferably 0.1% by mass or less.
• the proportion of the perfume having a boiling point of 120° C. or higher and 260° C. or lower in the total perfume is preferably 90% by mass or more, more preferably 95% by mass or more.
• the ratio of a perfume having a boiling point of 220°C or less and a boiling point of 120°C or more and 260°C or less to a diol having 2 to 4 carbon atoms is preferably from 0.00001 to 0.1, more preferably from 0.0001 to 0.01.
• composition of the present invention may contain, as long as the effects of the present invention are not impaired, for example, a viscosity modifier such as ethanol or isopropanol, benzazirconium chloride, dialkyldimethylammonium chloride, sodium hypochlorite, Chlorous acid water, chlorous acid water, nonvolatile antiviral and antibacterial agents such as glutaraldehyde, surfactants (excluding benzalkonium chloride and dialkyldimethylammonium chloride), chelating agents, moisturizers, lubricants, Builders, buffers, abrasives, electrolytes, bleaches, fragrances, dyes, foam control agents, corrosion inhibitors, essential oils, thickeners, pigments, gloss enhancers, enzymes, detergents, dispersants, polymers, silicones, hydrotropes Additives such as substances can be appropriately combined and contained.
• a viscosity modifier such as ethanol or isopropanol, benzazirconium chlor
• the content of the additive can be appropriately set within a range that does not impair the object of the present invention, but A1) a diol having a boiling point of 220 ° C. or less and having 2 to 4 carbon atoms, water and perfume in the composition
• the total content is preferably 80% by mass or more, more preferably 90% by mass or more.
• the non-volatile antiviral agent and antibacterial agent are preferably not contained. More preferably, it is 1% by mass or less, and preferably not substantially contained.
• ethanol is contained, from the viewpoint of preventing ignition, it is preferably 10% by mass or less, more preferably 5% by mass or less, even more preferably 1% by mass or less, and preferably not substantially contained.
• A1 a composition having a boiling point of 220° C. or less and a diol having 2 to 4 carbon atoms and water each having a specific concentration range is suitable for spraying. Upon contact, the virus can be inactivated. Furthermore, alkyl glyceryl ethers and specific fragrances are found to have the effect of inactivating viruses, and the combination of these with diols having a boiling point of 220° C. or less and having 2 to 4 carbon atoms improves the virus inactivating effect.
• a diol having a boiling point of 220°C or lower and having 2 to 4 carbon atoms can be an active ingredient for virus inactivation and can be used to produce a virus inactivation composition for liquid spray.
• compositions containing ⁇ 90% by mass, 10% to 60% by mass of water, and at least one selected from alkyl glyceryl ethers and specific fragrances is sprayed onto a subject suspected of viral contamination, the virus in the subject can be inactivated.
• the content of a diol having a boiling point of 220° C. or lower and having 2 to 4 carbon atoms in the composition is 40% by mass or more, preferably 50% by mass or more, from the viewpoint of virus inactivation, It is more preferably 60% by mass or more, more preferably 70% by mass or more, and from the viewpoint of spraying properties, it is 90% by mass or less, preferably 80% by mass or less.
• spraying properties means that the composition is less likely to remain in the discharge path within the container, and more of the compounded liquid is sprayed onto the object or space.
• water examples include tap water, distilled water, ion-exchanged water, and purified water.
• the content of water in the composition is 10% by mass or more, preferably 20% by mass or more from the viewpoint of spraying properties, and 60% by mass or less from the viewpoint of virus inactivation, It is preferably 50% by mass or less, more preferably 40% by mass or less.
• a diol with a boiling point of 220° C. or lower and a carbon number of 2 to 4 may be mixed with water to dissolve the diol having a boiling point of 220° C. or lower and a carbon number of 2 to 4 and water. It is preferable from the viewpoint of virus inactivation and the viewpoint of spray characteristics.
• the ratio of the diol having a boiling point of 220° C. or less and having 2 to 4 carbon atoms and water is, from the viewpoint of virus inactivation, the ratio of the diol having a boiling point of 220° C. or less to water and having 2 to 4 carbon atoms.
• the mass ratio [diol having a boiling point of 220° C. or less and having 2 to 4 carbon atoms/water] is preferably 0.6 to 9.0, more preferably 1.0 to 4.0, and still more preferably 1.5 to 4.0, more preferably 1.5 to 3.0.
• the viscosity of the composition at 20°C is preferably 2 to 40 mPa ⁇ s, more preferably 3 to 20 mPa ⁇ s, from the viewpoint of spraying properties. Viscosity can be measured with an ultrasonic viscometer. In situations where flammability is not a concern, ethanol and isopropyl alcohol can be included as viscosity modifiers, and when the composition contains these, the content is about 1 to 10% by mass in the composition. preferable.
• a preferred range for the spray amount of the composition is 0.1 g to 500 g/min, more preferably 10 g to 500 g/min, and even more preferably 20 g to 100 g/min. It is preferable to use an atomizing nozzle as a sprayer in order to obtain the above spray amount. Also, the amount of spray can be adjusted to 0.1 g to 1 g/min using an atomizing device such as a nebulizer, and 50 g to 500 g/min using a diffusion device such as a washer nozzle or a mist machine. Furthermore, when spraying in a closed space or a semi-closed space, the spray amount is preferably 50 g to 200 g/m 3 per target space.
• the range of the liquid pressure of the composition supplied to the nozzle of the sprayer is preferably 0.1 MPa or more, more preferably 0.15 MPa or more, from the viewpoint of facilitating spraying of even a viscous liquid. 2.5 MPa or less is preferable, and 0.7 MPa or less is more preferable.
• the foreign matter passage diameter (diameter of the maximum sphere that can pass through the minimum flow path in the nozzle) in the nozzle of the sprayer is preferably 0.05 mm or more, and is preferably 0.1 mm or more. It is more preferably 0.15 mm or more, and from the viewpoint of evenly spraying, it is preferably 0.5 mm or less, more preferably 0.4 mm or less, and even more preferably 0.3 mm or less.
• Viruses targeted for virus inactivation include all types of viruses, regardless of the type of nucleic acid (RNA, DNA) and whether or not they have an envelope.
• non-enveloped viruses include norovirus, poliovirus, echovirus, hepatitis A virus, hepatitis E virus, rhinovirus, astrovirus, rotavirus, coxsackievirus, enterovirus, and sapovirus.
• adenovirus which has DNA as the nucleic acid; B19 virus; papovavirus; human papillomavirus, and the like.
• viruses having an envelope are preferred, viruses having an envelope and having RNA as a nucleic acid are more preferred, and influenza virus, human coronavirus, SARS coronavirus, and SARS coronavirus-2 are more preferred.
• SARS coronavirus-2 severe acute respiratory syndrome coronavirus 2, SARS-CoV-2 is a SARS-related coronavirus that causes acute respiratory disease (COVID-19).
• virus inactivation means the action of reducing or eliminating virus activity and eliminating the ability to infect host cells.
• the virus-inactivating action can be confirmed, for example, by contacting the test product with the virus, infecting the host cell with the virus, and measuring the virus infection titer.
• the host cell may be any cell in which the virus of interest can proliferate.
• influenza virus for example, canine kidney cells (MDCK), African green monkey kidney epithelial cells (Vero), and duck embryonic stem cells.
• EB66 Human coronavirus
• HCT-8 human ileocecal adenocarcinoma cell
• VeroE6 African green monkey kidney epithelial cell
• Human liver cancer-derived cell line Huh7
• Objects that are concerned about virus contamination include the skin or mucous membranes of animals to which viruses adhere, hard or soft surfaces of inanimate objects, objects such as waste, and spaces where viruses splash or float.
• the surface of an inanimate object includes, for example, hard surfaces such as counters, sinks, restrooms, toilets, bathtubs, shower stands, floors, windows, doorknobs, walls, sewers, and pipes in homes and business facilities; Hard surfaces such as kitchen utensils, furniture, telephones, various appliances such as toys, tools, miscellaneous goods; and soft surfaces such as textile products (carpets, area rugs, curtains, fabric furniture, clothing, masks, etc.).
• waste examples include general waste (residues of foodstuffs, tissue paper, household waste such as masks), and industrial waste (sludge, manure, medical waste, etc.).
• general waste due to the waste is enclosed in a bag, the surface of the bag may be sprayed.
• general households such as dining kitchen rooms, bedrooms, children's rooms, bathrooms, toilets; stores, restaurants, inns, hospitals, workshops, livestock barns, factories, welfare facilities, educational facilities, movie theaters, museums, etc.
• a method of spraying the composition for example, it is filled in a known spray container such as a trigger spray container (direct pressure or pressure accumulation type), a dispenser type pump spray container, an aerosol spray container equipped with a pressure container, and the spray amount is adjusted.
• a method of appropriately adjusting and spraying can be mentioned.
• a pressurized air atomization spray device electric or manual
• a nebulizer an atomization device such as a diffuser
• a diffusion device such as a washer nozzle or a mist machine
• the object and the carrier that temporarily receives the composition are placed in a sealable space, and the composition is sprayed onto the carrier once. From there, a diol having a boiling point of 220°C or less and having 2 to 4 carbon atoms is vaporized and filled in a container.
• the timing of placing the carrier and the object in the sealable space and the timing of spraying the composition onto the carrier can be set arbitrarily.
• the carrier preferably has a boiling point of 220° C. or less and has a large surface area such as foamed sponge, non-woven fabric, or paper so that the diol having 2 to 4 carbon atoms can be easily vaporized.
• the garbage truck is equipped with an inlet into which the collected waste is thrown and a packing box into which the thrown-in waste is stored.
• the bag containing the waste may break.
• the worker puts the waste into the inlet, or when the worker rakes or pushes the waste into the packing box, the bag containing the waste may break.
• the virus will spread into the space near the input port, and there is concern that the workers of the garbage truck may be exposed to the virus. From the viewpoint of reducing the possibility of dust truck workers being exposed to the virus, it is preferable to inject the composition toward the inlet.
• the timing of spraying corresponds to the driving of the raking device or the pushing device.
• the composition is sprayed toward an object suspected of being contaminated with a virus, especially in a livestock barn, will be described.
• a plurality of sprayers on the ceiling of the barn and spray downward.
• the number of sprayers installed in the livestock barn is preferably 1 or more, more preferably 2 or more, and still more preferably 3 or more from the viewpoint of evenly spraying per 100 m 2 , and preferably 30 from an economical point of view. number or less, more preferably 20 or less, still more preferably 10 or less.
• the timing of spraying may be when viral contamination is detected (for example, avian influenza infection is confirmed) in the livestock barn, or it may be sprayed preventively.
• the average particle size of the sprayed particles is preferably 5 ⁇ m or more, more preferably 10 ⁇ m or more, and still more preferably 50 ⁇ m or more, from the viewpoint of being less susceptible to air currents so that the sprayed particles can be sprayed to the target location. It is preferably 500 ⁇ m or less, more preferably 200 ⁇ m or less, even more preferably 150 ⁇ m or less, and even more preferably 100 ⁇ m or less from the viewpoint of evenly spraying while suppressing the amount used and from the viewpoint of facilitating volatilization and inactivation of viruses in the space.
• the average particle size of the sprayed particles is the volume-based median size (D50) measured by a laser diffraction particle analyzer, and can be measured by the method described in Examples below.
• the composition is appropriately delivered to the surface of an object or space where virus contamination is a concern.
• Methods of spraying in an unheated state include spraying methods using trigger-type sprayers, nebulizers, misters, washer nozzles, etc., as described above.
• the temperature of the composition during spraying is preferably 20-40°C. That is, in the present invention, viruses can be inactivated in a non-heated state, and a safer virus inactivation method can be provided. Also, since spraying is used, the composition can be applied more precisely to the object.
• the distance between the ejection port that injects the composition and the object is preferably within 3 m from the viewpoint of efficiently delivering the composition to the object and maximizing virus inactivation. , and more preferably within 1 m.
• a diol having a boiling point of 220° C. or lower and having a carbon number of 2 to 4 is sprayed onto the carrier and then vaporized to inactivate the virus, it is preferably within 50 cm from the viewpoint of preventing the composition from adhering to the surroundings. , more preferably within 30 cm.
• the composition After spraying the composition, it is preferable to leave it for 30 minutes or more from the viewpoint of bringing the virus into contact with the diol having a boiling point of 220°C or less and having 2 to 4 carbon atoms and sufficiently demonstrating the virus inactivation effect.
• the concentration of a diol having a boiling point of 220° C. or less and a carbon number of 2 to 4 in the target space when performing virus inactivation in the space is preferably 20 ppm (volume concentration, hereinafter the same), more preferably 50 ppm or more, still more preferably 70 ppm or more, still more preferably 74 ppm or more, still more preferably 80 ppm or more, still more preferably 84 ppm or more, still more preferably 87 ppm or more.
• Influenza virus type A (A/Puerto Rico/8/1934, H1N1) strain was used as the test virus strain.
• a cotton ball impregnated with 1000 ⁇ L of 1,2-propanediol is placed on the bottom of a 1 L glass bottle manufactured by Tokyo Glass Instruments Co., Ltd., and the mixture is stirred at about 23° C. for 30 minutes at 500 rpm using a stirrer. operation was performed.
• a glass bottle with 1 ⁇ L of 1,2-propanediol aqueous solution (0.3 g/mL) dropped on the bottom is left in a constant temperature bath at 60° C. for 30 minutes, then at about 23° C. for 30 minutes using a rotor and a stirrer.
• the virus was collected in a medium, inoculated into MDCK cells (derived from canine renal tubular epithelial cells) previously cultured in a 12-well plate, and cultured at 37°C, 5% CO2 for about 18 hours. The number of foci formed was measured and the virus infection titer was determined.
• the concentration of the diol having a boiling point of 220° C. or lower and having 2 to 4 carbon atoms on the target surface is preferably 0.14 mg/cm 2 or more, and a more preferable concentration is 0. 0.20 mg/cm 2 or more, more preferably 0.25 mg/cm 2 or more.
• the concentration of the diol with a boiling point of 220°C or lower and having 2 to 4 carbon atoms on the target surface was measured by collecting the diol with a boiling point of 220°C or lower and having 2 to 4 carbon atoms on the surface using a glass or plastic petri dish. After that, extraction with a common solvent such as methanol, ethanol, or acetone is performed, and the concentration can be determined by gas chromatography/mass spectrometry (GC/MS).
• GC/MS gas chromatography/mass spectrometry
• the present invention further discloses the following aspects.
• a virus inactivating composition for liquid spray A1) A composition having a boiling point of 220° C. or lower and containing 40 to 90% by mass of a diol having 2 to 4 carbon atoms and 10 to 60% by mass of water.
• a composition having a boiling point of 220°C or less and containing 40 to 90% by mass of a diol having 2 to 4 carbon atoms and 10 to 60% by mass of water is applied to hard or soft inanimate objects that are concerned about virus contamination. Virus inactivation method by spraying onto surfaces or waste.
• a composition having a boiling point of 220° C. or lower and containing 40 to 90% by mass of a diol having 2 to 4 carbon atoms and 10 to 60% by mass of water is sprayed onto the carrier, and A1) the boiling point in the space is 220° C.
• a method for inactivating viruses in a target space comprising: vaporizing a composition from the carrier so that the concentration of the diol having 2 to 4 carbon atoms is 20 ppm or more;
• composition or method according to any one of ⁇ 1> to ⁇ 3>, further comprising A2) a glycol ether having a boiling point of 220° C. or lower and having 6 carbon atoms.
• A2) The composition has a boiling point of 220° C. or lower, and the content of the glycol ether having 6 carbon atoms is preferably 1% by mass or more, more preferably 3% by mass or more, and still more preferably 7% by mass or more. Also, preferably 40% by mass or less, more preferably 30% by mass or less, still more preferably 20% by mass or less, still more preferably 15% by mass or less, still more preferably 10% by mass or less. composition or method.
• ⁇ 6> The total amount of A1) a diol having a boiling point of 220° C. or lower and having 2 to 4 carbon atoms and A2) a glycol ether having a boiling point of 220° C. or lower and having 6 carbon atoms in the composition, A2) having a boiling point of 220° C.
• the mass ratio [A2)/(A1)+A2)] of the glycol ether having 6 carbon atoms is preferably 0.05 or more, more preferably 0.1 or more, and still more preferably 0.15 or more
• the composition or method according to ⁇ 4> or ⁇ 5> which is preferably 1.0 or less, more preferably 0.5 or less, and still more preferably 0.3 or less.
• the content of A3) alkyl glyceryl ether in the composition is preferably 0.1% by mass or more, more preferably 0.2% by mass or more, and still more preferably 0.4% by mass or more, and
• the mass ratio [A3)/A1) of A1) alkyl glyceryl ether to diol having a boiling point of 220°C or less and having 2 to 4 carbon atoms in the composition is preferably 0.001 or more, more preferably is 0.005 or more, more preferably 0.01 or more, and is preferably 0.1 or less, more preferably 0.05 or less, still more preferably 0.04 or less, and still more preferably 0.03 or less.
• composition A1 The ratio of the perfume to the diol having a boiling point of 220°C or less and having 2 to 4 carbon atoms (perfume/diol having a boiling point of 220°C or less and having 2 to 4 carbon atoms) is preferably 0.
• the composition or method according to ⁇ 10> or ⁇ 11> which is 0.00001 or more, more preferably 0.0001, and preferably 0.1 or less, more preferably 0.01 or less.
• ⁇ 13> The composition according to any one of ⁇ 1> to ⁇ 3>, further comprising A2) a glycol ether having a boiling point of 220° C. or less and having 6 carbon atoms, and at least one fragrance selected from the following: composition or method.
• ⁇ 15> Any of ⁇ 2>, ⁇ 4> to ⁇ 14>, wherein the hard or soft surface or waste of an inanimate object that is concerned about virus contamination is preferably a hard or soft surface or waste of a garbage truck The method described in . ⁇ 16> The method according to ⁇ 15>, wherein the composition is preferably sprayed from the upper part of the input port of the garbage truck toward the lower part and/or the inside of the side surface of the input port.
• Example 1-7 and Comparative Examples 1-3 Inactivation of influenza virus A virus inactivation test was performed in an acrylic container with a volume of 36 L (trade name, suction box 10 L 350 x 490 x 240 mm manufactured by AS ONE). In Examples 5, 6 and 7, further perfume compositions shown in Table 2 a) to c) were mixed. Influenza virus type A (A/Puerto Rico/8/1934, H1N1) strain was used as test virus strain.
• the composition is sprayed into a container for 1 minute x 3 times (interval of 2 minutes and 30 seconds) using a compressor nebulizer (NE-C803, manufactured by Omron, median spray particle size: about 5 ⁇ m), and at the same time, Influenza virus solution (5 ⁇ 10 6 FFU/mL) was sprayed with a compressor nebulizer for 3 minutes and then allowed to stand for 3 minutes.
• the total discharge amount for 1 minute ⁇ 3 times was 0.63 g to 0.79 g per 36 L.
• Viruses floating in the container space were collected in a total of 3 mL of serum-free medium in a bubbler for 2 minutes using an aspirator.
• Spray characteristic (%) (spray weight of test product/spray amount of Example 3) x 100 (2)
• the surface concentration of 1,2-propanediol dropped onto the target surface was determined by dropping 5 ml of methanol onto the compositions of Comparative Examples 1 to 3 and Examples 1 to 7 dropped onto the surface of a plastic petri dish with a diameter of 5 cm.
• the extract was determined from the abundance of 1,2-propanediol by the GC/MS method.
• the compositions of Examples 1 to 7 have good spraying properties, and by spraying the compositions, 30% or more of viruses on either or both of the airborne viruses on the surface of the object or in the space showed an inactivating effect. Furthermore, as shown in Table 2, the virus inactivating effect was improved in Examples 5, 6, and 7, in which a flavoring agent having a virus inactivating effect was mixed.
• Example 8 A composition with a 1,2-propanediol concentration of 80% by mass and a water concentration of 20% by mass, assuming a garbage truck input port (about 1 m 3 for a medium-sized vehicle) where there is a risk of virus infection when garbage bursts. was used to test whether an effective concentration was reached. A 2m3 stainless steel space with a door was used. A total of 40 g of the composition was sprayed on the three sides and the bottom using a trigger spray. The volume-based median diameter (D50) of the sprayed composition of Example 8 measured under the following conditions was 89.0 ⁇ m.
• D50 volume-based median diameter
• ⁇ D50 measurement conditions Laser diffraction particle size distribution analyzer (manufacturer: Malvern, Spraytech), lens used: 300 mm Spray direction: Spray in the horizontal direction Measurement area: Place the measurement device so that the measurement area is 15 cm horizontally (spray direction) from the nozzle of the sprayer Sampling time: 1 s After spraying, the door was closed and left alone. During gas analysis, the 1,2-propanediol concentration in the space was measured by inserting a gas collection tube (Tenax (registered trademark) TA: manufactured by GERSTEL) through the hole in the door, and sucking the air inside at a rate of 100 mL/min. It was collected at minute aspiration.
• a gas collection tube Teenax (registered trademark) TA: manufactured by GERSTEL
• the collected air was analyzed using a gas chromatograph mass spectrometer (GC/MS) equipped with a thermal desorption system.
• the GC/MS analysis conditions were as follows: DB-WAX (manufactured by Agilent) as a GC column, and temperature elevation conditions of 40°C (held for 3 minutes) -6°C/min -70°C -3°C/min -240°C.
• P. G. was determined by injecting a fixed amount of diluted methanol solution into a Tenax adsorption tube, analyzing it with a GC/MS apparatus with a thermal desorption system in the same manner as described above, and creating a calibration curve from the detected values. As a result, it was confirmed that the 1,2-propanediol concentration in the space reached 84 ppm after 30 minutes.
• Example 9 Assuming a toy box where there is a risk of virus infection through kindergarteners and children, use a composition with a 1,2-propanediol concentration of 80% by mass and a water concentration of 20% by mass to determine whether an effective concentration is reached. tested. Using a commercially available 68 L polypropylene storage box, 10 sponges were placed at the bottom, and a total of 16 g (20 times) or 24 g (30 times) of the composition was sprayed from 30 cm above the sponge using a trigger spray. Furthermore, after laying a wire mesh, 20 toys were stuffed from the top, covered and left as they were. As in Example 8, the 1,2-propanediol concentration in the storage box was measured. As a result, it was confirmed that after 6 hours, the concentration of 1,2-propanediol in the storage box reached 50 ppm after 20 sprays and 74 ppm after 30 sprays.
• Examples 10-12 Assuming spraying to the garbage inlet of a garbage truck, the spraying characteristics of the sprayer were tested.
• the sprayer body uses Koshin Mister Auto 2.5L, which is a pressurized air atomization spray device (maximum pressure about 0.3 MPa), and the tip nozzle part is an atomization nozzle (Ikeuchi Co., empty cone nozzle KB80125N: foreign matter passage diameter 0.3 mm).
• As spray liquids blended liquids of Examples 10 to 12 shown in Table 3 were prepared. As a result, all of them could be sprayed conically without any problem.
• Example 13 is a mixed liquid having a 1,2-propanediol concentration of 60% by mass and a water concentration of 40% by mass
• Example 14 is a mixture having a 1,2-propanediol concentration of 60% by mass and diethylene glycol.
• Example 14 showed a finer and more stable spray pattern, with a diameter of 20 cm for Example 13 and 23 cm for Example 14. It was shown that the addition of A2) component can improve the conical spray pattern.
• the volume-based median diameter (D50) of the liquid mixture of Example 13 sprayed measured under the following conditions was 75.0 ⁇ m.
• ⁇ D50 measurement conditions Laser diffraction particle size distribution analyzer (manufacturer: Malvern, Spraytech), lens used: 300 mm Spray direction: Spray in the horizontal direction Measurement area: Place the measurement device so that the measurement area is 15 cm horizontally (spray direction) from the nozzle of the sprayer Sampling time: 1 s
• the blended solution of Example 13 was sprayed using the compressor nebulizer (NE-C803, manufactured by Omron Co., Ltd.) used in Example 1.
• a metallic stand was used in the same manner as described above, and the nozzle portion was fixed to a height of 30 cm.
• the ejection direction was horizontal, so a hose bent into a crank shape was connected so that the spray could be vertically downward, and the spray was vertically downward for 10 seconds.
• the sprayed particles diffused about 20 cm from the ejection port, and no stable spray pattern was observed on the falling surface.
• the volume-based median diameter (D50) of the spray measured under the above conditions was 4 ⁇ m.
• Examples 15 and 16 virus inactivation test by spraying.
• Method A virus inactivation test was performed in a 36 L volume acrylic container (trade name, suction box 10 L, 350 x 490 x 240 mm, manufactured by AS ONE).
• Influenza virus type A A/Puerto Rico/8/1934, H1N1 strain was used as test virus strain.
• Influenza virus solution (1.8 ⁇ 10 8 FFU/mL) was sprayed with a compressor nebulizer (NE-C803, manufactured by Omron).
• the composition contains 1,2-propanediol (PG, manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.) and 2-ethylhexyl glyceryl ether (GE-EH, manufactured by Fujifilm Wako) so that the concentration (% by mass) shown in Table 4 is obtained. (manufactured by Kojunyaku Co., Ltd.) was mixed with water and sprayed into the acrylic container from the hole in the center of the lid with a sprayer. Koshin's Mr.
• PG 1,2-propanediol
• GE-EH 2-ethylhexyl glyceryl ether
• Auto 2.5L was used for the main body of the sprayer (maximum pressure: about 0.3 MPa), and the tip nozzle was changed to a fine mist nozzle (Ikeuchi Co., Ltd., empty cone nozzle KB8010N: foreign matter passage diameter 0.25 mm). .
• the composition was sprayed for 5 seconds, the virus for 10 seconds, the composition for 5 seconds, the virus for 10 seconds, and the composition for 5 seconds, and then left to stand in an acrylic box. 15 minutes after the start of the test, the virus that had fallen onto four 3-cm-diameter dishes (manufactured by AGC Techno Glass Co., Ltd.) placed in advance on the bottom of the acrylic container was added to 1.2 mL of serum-free medium per dish.
• Viscosity measurement conditions > ⁇ Equipment Ultrasonic viscometer (Maruyasu Kogyo Co., Ltd., Viscotec) ⁇ Measurement temperature 20°C ⁇ Filling amount to the sensor part: about 0.2g
• the volume-based median diameter (D50) of the sprayed liquid of each composition shown in Table 4 was measured according to the following conditions.
• ⁇ D50 measurement conditions> Laser diffraction particle size distribution analyzer (manufacturer: Malvern, Spraytech), lens used: 300 mm Spray direction: Spray in the horizontal direction Measurement area: Place the measurement device so that the measurement area is 15 cm horizontally (spray direction) from the nozzle of the sprayer Sampling time: 1 s
• Results represent the average of 4 dishes. A higher value indicates a better virus inactivation effect.
• the detection limit of this test was 99.97% (3.56 Log reduction), and the sample (PG 40% by mass + GE-EH 0.7% by mass) in which the amount of virus titer detected was below the detection limit. showed an activity value of >99.97 (>3.56).
• Methods Influenza virus type A (A/Puerto Rico/8/1934, H1N1) strain was used as the test virus strain.
• a sample solution was prepared so that the final concentration (v/v%) was as shown in Table 4 below.
• 5 ⁇ L of influenza virus solution (6.0 ⁇ 10 5 FFU) in Hybridoma-SFM medium (Thermo Fisher Scientific) and 45 ⁇ L of sample solution were allowed to react at room temperature (about 23° C.) for 5 minutes.
• Reference Example 2 Fragrance indicator test 1 Method Compositions A to M prepared by mixing 1,2-propanediol (P.G.) with water and perfume to the concentrations shown in Table 6 were placed in a trigger container and tested as scent indicators. rice field. Specifically, a 40 L garbage bag was filled with paper waste and left standing outdoors (at a temperature of about 15°C). Spray the composition 10 times or 20 times on the surface of each garbage bag diagonally from above, and measure the strength of the scent immediately after, 30 minutes, and 60 minutes after spraying from a distance of 30 cm according to the following 6-step odor intensity display method.
• P.G. 1,2-propanediol
• Reference Example 3 Inactivation of influenza virus in fragrance 1.
• Influenza virus type A (A/Puerto Rico/8/1934, H1N1) strain was used as the test virus strain.
• a cotton ball was impregnated with 75 ⁇ L of the perfume shown in Table 7, and the cap of a 15 mL glass bottle (Maruem Co., Ltd.) was adhered and sealed with double-sided tape, and filled for 30 minutes.
• 1.5 ⁇ L (8.3 ⁇ 10 5 FFU) of influenza virus was allowed to dry on the lid of a cryovial (Thermo Fisher Scientific) for 30 minutes.
• the virus-attached vial cap was placed in a glass bottle, and the virus was allowed to react with the flavoring compound at room temperature (approximately 23° C.) for 30 minutes.
• the fragrance compound was placed on the lid of the bottle, and the virus was placed on the bottom of the bottle, so that the fragrance compound and the virus did not come into direct contact with each other.
• Reference Example 4 Inactivation of coronavirus of the product of the present invention 1.
• Methods SARS-CoV-2 JPN/Kanagawa/KUH003 was used as test virus strain. 3 ⁇ L of the virus suspension was added to 27 ⁇ L of the solution having the composition shown in Table 7 and mixed. After 5 minutes of reaction at room temperature, fetal bovine serum 2%, penicillin 100 U/mL, streptomycin 100 ⁇ g/mL, and geneticin G418 1 mg/mL were added to a cell maintenance medium (Dulbecco's Modified Eagle Medium (Nacalai Tesque, Inc.) after 5 minutes. ) and serially diluted 3-fold with cell maintenance medium.
• a cell maintenance medium Dulbecco's Modified Eagle Medium (Nacalai Tesque, Inc.) after 5 minutes.
• VeroE6/TMPRSS2 cells previously cultured in a 96-well plate were inoculated with 100 ⁇ L of the diluted reaction solution per well and infected at 37° C., 5% CO 2 for about 1 hour. After infection, all diluents were removed, the cells were washed twice with cell maintenance medium, fresh cell maintenance medium was added, and the cells were cultured for 3 days. After culturing, the presence or absence of morphological changes in the cells (cytopathic effect: CPE) was observed using an inverted phase-contrast microscope, and the 50% tissue culture infectious dose (TCID 50 ) was calculated by the Behrens-Karber method per 1 mL of the reaction solution.
• CPE tissue culture infectious dose
• Virus inactivation effect log TCID 50 / mL of control - log TCID 50 / mL of reaction solution / mL infectious titer of control] (4)

Landscapes

• Life Sciences & Earth Sciences (AREA)
• Health & Medical Sciences (AREA)
• General Health & Medical Sciences (AREA)
• Wood Science & Technology (AREA)
• Engineering & Computer Science (AREA)
• Plant Pathology (AREA)
• Pest Control & Pesticides (AREA)
• Zoology (AREA)
• Environmental Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Dentistry (AREA)
• Agronomy & Crop Science (AREA)
• Toxicology (AREA)
• Animal Behavior & Ethology (AREA)
• Epidemiology (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Public Health (AREA)
• Veterinary Medicine (AREA)
• Dispersion Chemistry (AREA)
• General Chemical & Material Sciences (AREA)
• Organic Chemistry (AREA)
• Oil, Petroleum & Natural Gas (AREA)
• Agricultural Chemicals And Associated Chemicals (AREA)
• Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
• Micro-Organisms Or Cultivation Processes Thereof (AREA)
• Medicinal Preparation (AREA)

Priority Applications (1)

Applications Claiming Priority (6)

Publications (1)

Family

ID=83640765

Family Applications (1)

Country Status (3)

Families Citing this family (1)

Citations (4)

Family Cites Families (11)

• 2022
  • 2022-04-14 TW TW111114327A patent/TW202308512A/zh unknown
  • 2022-04-14 WO PCT/JP2022/017875 patent/WO2022220290A1/ja not_active Ceased
  • 2022-04-14 JP JP2022067238A patent/JP2022164624A/ja active Pending

Patent Citations (4)

Also Published As

Similar Documents

Legal Events