WO2022149865A3 - GlxR 단백질 변이체 또는 이를 이용한 쓰레오닌 생산방법 - Google Patents

GlxR 단백질 변이체 또는 이를 이용한 쓰레오닌 생산방법 Download PDF

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Publication number
WO2022149865A3
WO2022149865A3 PCT/KR2022/000204 KR2022000204W WO2022149865A3 WO 2022149865 A3 WO2022149865 A3 WO 2022149865A3 KR 2022000204 W KR2022000204 W KR 2022000204W WO 2022149865 A3 WO2022149865 A3 WO 2022149865A3
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WIPO (PCT)
Prior art keywords
glxr
protein variant
same
production method
threonine production
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PCT/KR2022/000204
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English (en)
French (fr)
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WO2022149865A2 (ko
Inventor
소이슬
권수연
이광우
심지현
Original Assignee
씨제이제일제당 (주)
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Publication date
Application filed by 씨제이제일제당 (주) filed Critical 씨제이제일제당 (주)
Priority to AU2022206623A priority Critical patent/AU2022206623B2/en
Priority to MX2023008105A priority patent/MX2023008105A/es
Priority to EP22736842.0A priority patent/EP4276107A2/en
Priority to JP2023541941A priority patent/JP2024503049A/ja
Priority to CN202280009585.6A priority patent/CN116888141A/zh
Publication of WO2022149865A2 publication Critical patent/WO2022149865A2/ko
Publication of WO2022149865A3 publication Critical patent/WO2022149865A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 출원은 GlxR 단백질 변이체 또는 이를 이용한 쓰레오닌 생산방법에 관한 것으로서, 일 예에 따른 GlxR 단백질 변이체는 미생물에 도입되어, 부산물의 생산량을 감소시키고, 쓰레오닌 생산능을 현저히 증가시킬 수 있다.
PCT/KR2022/000204 2021-01-11 2022-01-06 GlxR 단백질 변이체 또는 이를 이용한 쓰레오닌 생산방법 WO2022149865A2 (ko)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2022206623A AU2022206623B2 (en) 2021-01-11 2022-01-06 Glxr protein variant or threonine production method using same
MX2023008105A MX2023008105A (es) 2021-01-11 2022-01-06 Variante de proteina glxr o metodo de produccion de treonina usando la misma.
EP22736842.0A EP4276107A2 (en) 2021-01-11 2022-01-06 Glxr protein variant or threonine production method using same
JP2023541941A JP2024503049A (ja) 2021-01-11 2022-01-06 ＧｌｘＲ蛋白質変異体またはこれを利用したスレオニン生産方法
CN202280009585.6A CN116888141A (zh) 2021-01-11 2022-01-06 Glxr蛋白变体或使用其生产苏氨酸的方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020210003609A KR102527895B1 (ko) 2021-01-11 2021-01-11 GlxR 단백질 변이체 또는 이를 이용한 쓰레오닌 생산방법
KR10-2021-0003609 2021-01-11

Publications (2)

Publication Number Publication Date
WO2022149865A2 WO2022149865A2 (ko) 2022-07-14
WO2022149865A3 true WO2022149865A3 (ko) 2022-08-25

Family

ID=82358248

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2022/000204 WO2022149865A2 (ko) 2021-01-11 2022-01-06 GlxR 단백질 변이체 또는 이를 이용한 쓰레오닌 생산방법

Country Status (8)

Country Link
EP (1) EP4276107A2 (ko)
JP (1) JP2024503049A (ko)
KR (1) KR102527895B1 (ko)
CN (1) CN116888141A (ko)
AR (1) AR124580A1 (ko)
AU (1) AU2022206623B2 (ko)
MX (1) MX2023008105A (ko)
WO (1) WO2022149865A2 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116120409B (zh) * 2022-11-03 2023-09-26 华南理工大学 一种imp生物传感器及其构建方法与应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050026388A (ko) * 2002-05-16 2005-03-15 바스프 악티엔게젤샤프트 코리네박테리움 글루타미쿰에서의 리신 합성을 위한리프레서 ｇｌｘＲ의 용도
KR20080082880A (ko) * 2007-03-09 2008-09-12 한국과학기술원 부위특이적 변이에 의해 제조된 고수율의 ｌ－쓰레오닌생산균주 및 이를 이용한 ｌ－쓰레오닌의 제조방법
KR20110058731A (ko) * 2009-11-25 2011-06-01 한국과학기술원 Ｌ­이소루신 고생성능을 가지는 변이미생물 및 이의 제조방법
WO2020138815A1 (ko) * 2018-12-26 2020-07-02 대상 주식회사 L-아미노산을 생산하는 대장균 변이주 또는 코리네박테리움 글루타미컴 변이주 및 이를 이용한 l-아미노산의 생산 방법
KR20200130567A (ko) * 2019-05-09 2020-11-19 씨제이제일제당 (주) L-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산을 생산하는 방법

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10359661A1 (de) * 2003-12-18 2005-07-28 Basf Ag Genvarianten die für Proteine aus dem Stoffwechselweg von Feinchemikalien codieren
WO2008033001A1 (en) 2006-09-15 2008-03-20 Cj Cheiljedang Corporation A corynebacteria having enhanced l-lysine productivity and a method of producing l-lysine using the same
PL3144385T3 (pl) 2014-09-05 2020-12-14 Cj Cheiljedang Corporation Mikroorganizm o ulepszonej produktywności l-treoniny i sposób produkcji l-treoniny z jego wykorzystaniem
KR101947959B1 (ko) 2018-05-28 2019-02-13 씨제이제일제당 (주) 변이형 호모세린 디하이드로게나제 및 이를 이용한 호모세린 또는 호모세린 유래 l-아미노산의 생산 방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050026388A (ko) * 2002-05-16 2005-03-15 바스프 악티엔게젤샤프트 코리네박테리움 글루타미쿰에서의 리신 합성을 위한리프레서 ｇｌｘＲ의 용도
KR20080082880A (ko) * 2007-03-09 2008-09-12 한국과학기술원 부위특이적 변이에 의해 제조된 고수율의 ｌ－쓰레오닌생산균주 및 이를 이용한 ｌ－쓰레오닌의 제조방법
KR20110058731A (ko) * 2009-11-25 2011-06-01 한국과학기술원 Ｌ­이소루신 고생성능을 가지는 변이미생물 및 이의 제조방법
WO2020138815A1 (ko) * 2018-12-26 2020-07-02 대상 주식회사 L-아미노산을 생산하는 대장균 변이주 또는 코리네박테리움 글루타미컴 변이주 및 이를 이용한 l-아미노산의 생산 방법
KR20200130567A (ko) * 2019-05-09 2020-11-19 씨제이제일제당 (주) L-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산을 생산하는 방법

Also Published As

Publication number Publication date
AU2022206623B2 (en) 2024-01-04
JP2024503049A (ja) 2024-01-24
CN116888141A (zh) 2023-10-13
KR20220101509A (ko) 2022-07-19
MX2023008105A (es) 2023-07-17
AR124580A1 (es) 2023-04-12
KR102527895B1 (ko) 2023-04-28
AU2022206623A1 (en) 2023-06-29
EP4276107A2 (en) 2023-11-15
WO2022149865A2 (ko) 2022-07-14

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