AR124580A1 - Variante de proteína glxr o método de producción de treonina utilizando la misma - Google Patents
Variante de proteína glxr o método de producción de treonina utilizando la mismaInfo
- Publication number
- AR124580A1 AR124580A1 ARP220100020A ARP220100020A AR124580A1 AR 124580 A1 AR124580 A1 AR 124580A1 AR P220100020 A ARP220100020 A AR P220100020A AR P220100020 A ARP220100020 A AR P220100020A AR 124580 A1 AR124580 A1 AR 124580A1
- Authority
- AR
- Argentina
- Prior art keywords
- amino acid
- acid sequence
- glxr
- protein variant
- phenylalanine
- Prior art date
Links
- 102100030648 Glyoxylate reductase/hydroxypyruvate reductase Human genes 0.000 title 1
- 101001010442 Homo sapiens Glyoxylate reductase/hydroxypyruvate reductase Proteins 0.000 title 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 title 1
- 239000004473 Threonine Substances 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 150000001413 amino acids Chemical group 0.000 abstract 9
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 abstract 4
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 abstract 4
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 abstract 4
- 239000004472 Lysine Substances 0.000 abstract 4
- 101100069458 Mus musculus Grhpr gene Proteins 0.000 abstract 4
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 abstract 4
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 abstract 3
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 abstract 3
- 235000004279 alanine Nutrition 0.000 abstract 3
- 238000006467 substitution reaction Methods 0.000 abstract 3
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 abstract 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 abstract 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 abstract 1
- 235000001014 amino acid Nutrition 0.000 abstract 1
- 235000003704 aspartic acid Nutrition 0.000 abstract 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 abstract 1
- HHLFWLYXYJOTON-UHFFFAOYSA-N glyoxylic acid Chemical compound OC(=O)C=O HHLFWLYXYJOTON-UHFFFAOYSA-N 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/15—Corynebacterium
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Reivindicación 1: Una variante de proteína GlxR (regulador de derivación de glioxilato) que comprende una mutación seleccionada del grupo que consta de (1) a (4) a continuación: (1) sustitución de la secuencia de aminoácidos correspondiente a la posición de aminoácidos 45 del N-terminal en la secuencia de aminoácidos de SEQ ID Nº 1 con alanina, serina, fenilalanina o lisina; (2) sustitución de la secuencia de aminoácidos correspondiente a la posición 66 de aminoácidos del N-terminal en la secuencia de aminoácidos de SEQ ID Nº 1 con alanina, fenilalanina o lisina; (3) sustitución de la secuencia de aminoácidos correspondiente a la posición de aminoácido 166 del N-terminal en la secuencia de aminoácidos de SEQ ID Nº 1 con ácido aspártico, fenilalanina o lisina; y (4) sustitución de la secuencia de aminoácidos correspondiente a la posición de aminoácidos 168 del N-terminal en la secuencia de aminoácidos de SEQ ID Nº 1 con alanina, fenilalanina o lisina. Reivindicación 2: Un polinucleótido que codifica la variante de la proteína GlxR de la reivindicación 1. Reivindicación 3: Un microorganismo, que comprende uno o más tipos seleccionados del grupo que consiste en la variante de la proteína GlxR de la reivindicación 1 y un polinucleótido que codifica la variante de la proteína GlxR.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020210003609A KR102527895B1 (ko) | 2021-01-11 | 2021-01-11 | GlxR 단백질 변이체 또는 이를 이용한 쓰레오닌 생산방법 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR124580A1 true AR124580A1 (es) | 2023-04-12 |
Family
ID=82358248
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP220100020A AR124580A1 (es) | 2021-01-11 | 2022-01-06 | Variante de proteína glxr o método de producción de treonina utilizando la misma |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP4276107A2 (es) |
| KR (1) | KR102527895B1 (es) |
| CN (1) | CN116888141A (es) |
| AR (1) | AR124580A1 (es) |
| AU (1) | AU2022206623B2 (es) |
| MX (1) | MX2023008105A (es) |
| WO (1) | WO2022149865A2 (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116120409B (zh) * | 2022-11-03 | 2023-09-26 | 华南理工大学 | 一种imp生物传感器及其构建方法与应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1362866A1 (en) * | 2002-05-16 | 2003-11-19 | Basf Aktiengesellschaft | Use of the repressor glxR for the synthesis of lysine in Corynebacterium glutamicum |
| DE10359661A1 (de) * | 2003-12-18 | 2005-07-28 | Basf Ag | Genvarianten die für Proteine aus dem Stoffwechselweg von Feinchemikalien codieren |
| KR100924065B1 (ko) | 2006-09-15 | 2009-10-27 | 씨제이제일제당 (주) | Ｌ－라이신 생산능이 향상된 코리네박테리아 및 그를 이용한 ｌ－라이신 생산 방법 |
| KR100858913B1 (ko) * | 2007-03-09 | 2008-09-17 | 한국과학기술원 | 부위특이적 변이에 의해 제조된 고수율의 ｌ－쓰레오닌생산균주 및 이를 이용한 ｌ－쓰레오닌의 제조방법 |
| KR101270149B1 (ko) * | 2009-11-25 | 2013-06-04 | 한국과학기술원 | Ｌ이소루신 고생성능을 가지는 변이미생물 및 이의 제조방법 |
| HUE050610T2 (hu) | 2014-09-05 | 2021-01-28 | Cj Cheiljedang Corp | Javított L-treonin termelésére képes mikroorganizmus, és eljárás L-treonin elõállítására annak alkalmazásával |
| KR101947959B1 (ko) | 2018-05-28 | 2019-02-13 | 씨제이제일제당 (주) | 변이형 호모세린 디하이드로게나제 및 이를 이용한 호모세린 또는 호모세린 유래 l-아미노산의 생산 방법 |
| EP3904521A4 (en) * | 2018-12-26 | 2022-03-23 | Daesang Corporation | L-AMINO ACID-PRODUCING VARIANT STRAIN OF E. COLI OR CORYNEBACTERIUM GLUTAMICUM, AND METHODS OF PREPARATION OF L-AMINO ACIDS USING THE SAME |
| KR102221040B1 (ko) * | 2019-05-09 | 2021-03-03 | 씨제이제일제당 주식회사 | L-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산을 생산하는 방법 |
-
2021
- 2021-01-11 KR KR1020210003609A patent/KR102527895B1/ko active IP Right Grant
-
2022
- 2022-01-06 WO PCT/KR2022/000204 patent/WO2022149865A2/ko active Application Filing
- 2022-01-06 EP EP22736842.0A patent/EP4276107A2/en active Pending
- 2022-01-06 AR ARP220100020A patent/AR124580A1/es unknown
- 2022-01-06 MX MX2023008105A patent/MX2023008105A/es unknown
- 2022-01-06 CN CN202280009585.6A patent/CN116888141A/zh active Pending
- 2022-01-06 AU AU2022206623A patent/AU2022206623B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2022149865A2 (ko) | 2022-07-14 |
| CN116888141A (zh) | 2023-10-13 |
| WO2022149865A3 (ko) | 2022-08-25 |
| MX2023008105A (es) | 2023-07-17 |
| EP4276107A2 (en) | 2023-11-15 |
| AU2022206623B2 (en) | 2024-01-04 |
| KR102527895B1 (ko) | 2023-04-28 |
| KR20220101509A (ko) | 2022-07-19 |
| AU2022206623A1 (en) | 2023-06-29 |
| JP2024503049A (ja) | 2024-01-24 |
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