WO2021242408A3 - Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control - Google Patents

Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control Download PDF

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Publication number
WO2021242408A3
WO2021242408A3 PCT/US2021/025487 US2021025487W WO2021242408A3 WO 2021242408 A3 WO2021242408 A3 WO 2021242408A3 US 2021025487 W US2021025487 W US 2021025487W WO 2021242408 A3 WO2021242408 A3 WO 2021242408A3
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WO
WIPO (PCT)
Prior art keywords
production
xylitol
compositions
methods
metabolic control
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Application number
PCT/US2021/025487
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French (fr)
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WO2021242408A2 (en
Inventor
Michael David Lynch
Shuai LI
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Duke University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Duke University filed Critical Duke University
Priority to CN202180037240.7A priority Critical patent/CN115916990A/en
Priority to KR1020227038199A priority patent/KR20220164007A/en
Priority to EP21811936.0A priority patent/EP4127202A4/en
Priority to CA3179180A priority patent/CA3179180A1/en
Priority to BR112022019942A priority patent/BR112022019942A2/en
Priority to US17/995,360 priority patent/US20230183757A1/en
Priority to AU2021278792A priority patent/AU2021278792A1/en
Priority to IL297008A priority patent/IL297008A/en
Priority to JP2022560304A priority patent/JP2023528727A/en
Publication of WO2021242408A2 publication Critical patent/WO2021242408A2/en
Publication of WO2021242408A3 publication Critical patent/WO2021242408A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01307—D-Xylose reductase (1.1.1.307)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/0101—Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific)(1.3.1.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The present disclosure is related to genetically engineered microbial strains and related bioprocesses for the production of xylitol. Specifically, the use of dynamically controlled synthetic metabolic valves to reduce the activity of certain enzymes, leads to increased xylitol production in a two-stage process.
PCT/US2021/025487 2020-04-03 2021-04-02 Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control WO2021242408A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
CN202180037240.7A CN115916990A (en) 2020-04-03 2021-04-02 Methods and compositions for the production of xylitol from xylose using dynamic metabolic control
KR1020227038199A KR20220164007A (en) 2020-04-03 2021-04-02 Methods and compositions for producing xylitol from xylose using dynamic metabolic control
EP21811936.0A EP4127202A4 (en) 2020-04-03 2021-04-02 Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control
CA3179180A CA3179180A1 (en) 2020-04-03 2021-04-02 Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control
BR112022019942A BR112022019942A2 (en) 2020-04-03 2021-04-02 E. COLI MICROORGANISMS GENETICALLY MODIFIED TO PRODUCE XYLITOL FROM XYLOSE AND MULTI-STAGE FERMENTATION BIOPROCESSES TO PRODUCE XYLITOL
US17/995,360 US20230183757A1 (en) 2020-04-03 2021-04-02 Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control
AU2021278792A AU2021278792A1 (en) 2020-04-03 2021-04-02 Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control
IL297008A IL297008A (en) 2020-04-03 2021-04-02 Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control
JP2022560304A JP2023528727A (en) 2020-04-03 2021-04-02 Methods and compositions for producing xylitol from xylose using dynamic metabolic control

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063004740P 2020-04-03 2020-04-03
US63/004,740 2020-04-03

Publications (2)

Publication Number Publication Date
WO2021242408A2 WO2021242408A2 (en) 2021-12-02
WO2021242408A3 true WO2021242408A3 (en) 2022-04-07

Family

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PCT/US2021/025487 WO2021242408A2 (en) 2020-04-03 2021-04-02 Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control

Country Status (9)

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EP (1) EP4127202A4 (en)
JP (1) JP2023528727A (en)
KR (1) KR20220164007A (en)
CN (1) CN115916990A (en)
AU (1) AU2021278792A1 (en)
BR (1) BR112022019942A2 (en)
CA (1) CA3179180A1 (en)
IL (1) IL297008A (en)
WO (1) WO2021242408A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023192368A1 (en) * 2022-03-30 2023-10-05 The Regents Of The University Of California Hydrogelated cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140322779A1 (en) * 2011-11-11 2014-10-30 Genomatica, Inc. Eukaryotic organisms and methods for increasing the availability of cytosolic acetyl-coa, and for the producing 1,3-butanediol
WO2016053397A2 (en) * 2014-06-11 2016-04-07 Duke University Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018156646A1 (en) * 2017-02-21 2018-08-30 Duke University Compositions and methods for robust dynamic metabolic control
WO2019246488A1 (en) * 2018-06-21 2019-12-26 Duke University Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140322779A1 (en) * 2011-11-11 2014-10-30 Genomatica, Inc. Eukaryotic organisms and methods for increasing the availability of cytosolic acetyl-coa, and for the producing 1,3-butanediol
WO2016053397A2 (en) * 2014-06-11 2016-04-07 Duke University Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHIN ET AL.: "Analysis of NADPH Supply During Xylitol Production by Engineered Escherichia coli", BIOTECHNOLOGY AND BIOENGINEERING, vol. 102, no. 1, 1 January 2009 (2009-01-01), pages 209 - 220, XP055313140, DOI: 10.1002/bit.22060 *
GAO ET AL.: "Programmable biomolecular switches for rewiring flux in Escherichia coli", NATURE COMMUNICATIONS, vol. 10, no. 1, 1 December 2019 (2019-12-01), XP055929815, DOI: 10.1038/s41467-019-11793-7 *
HASPER ET AL.: "T he Aspergillus niger transcriptional activator XlnR, which is involved in the degradation of the polysaccharides xylan and cellulose, also regulates D-xylose reductase gene expression", MOLECULAR MICROBIOLOGY, vol. 36, no. 1, April 2000 (2000-04-01), pages 193 - 200, XP002360460, DOI: 10.1046/j.1365-2958.2000.01843.x *

Also Published As

Publication number Publication date
WO2021242408A2 (en) 2021-12-02
IL297008A (en) 2022-12-01
AU2021278792A1 (en) 2022-11-03
BR112022019942A2 (en) 2022-12-13
CA3179180A1 (en) 2021-12-02
EP4127202A4 (en) 2023-09-13
KR20220164007A (en) 2022-12-12
JP2023528727A (en) 2023-07-06
CN115916990A (en) 2023-04-04
EP4127202A2 (en) 2023-02-08

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