WO2020159169A3 - 당화된 ag85a 단백질을 포함하는 결핵 예방용 백신 조성물 및 이의 제조방법 - Google Patents

당화된 ag85a 단백질을 포함하는 결핵 예방용 백신 조성물 및 이의 제조방법 Download PDF

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WO2020159169A3
WO2020159169A3 PCT/KR2020/001230 KR2020001230W WO2020159169A3 WO 2020159169 A3 WO2020159169 A3 WO 2020159169A3 KR 2020001230 W KR2020001230 W KR 2020001230W WO 2020159169 A3 WO2020159169 A3 WO 2020159169A3
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glycosylated
protein
vaccine composition
ag85a
ag85a protein
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PCT/KR2020/001230
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WO2020159169A2 (ko
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이용직
손은주
신성재
김홍민
조관구
오명열
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주식회사 바이오앱
연세대학교 산학협력단
주식회사 큐라티스
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Priority to EP20748794.3A priority Critical patent/EP3919075A4/en
Priority to US17/426,275 priority patent/US20220088167A1/en
Priority to JP2021543360A priority patent/JP7233662B2/ja
Priority to CN202080011317.9A priority patent/CN113365655A/zh
Publication of WO2020159169A2 publication Critical patent/WO2020159169A2/ko
Publication of WO2020159169A3 publication Critical patent/WO2020159169A3/ko

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/04—Mycobacterium, e.g. Mycobacterium tuberculosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A61P31/06—Antibacterial agents for tuberculosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/57—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/57—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2
    • A61K2039/572—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2 cytotoxic response
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

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  • Chemical & Material Sciences (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract

본 발명은 당화된 Ag85A 단백질을 포함하는 결핵 예방용 백신 조성물, 상기 단백질의 제조를 위한 벡터, 상기 벡터를 이용한 형질전환체, 및 상기 형질전환체를 이용하여 당화된 Ag85A 단백질을 생산하는 방법 등에 관한 것으로, 본 발명의 당화된 Ag85A 단백질을 포함하는 백신 조성물은, 결핵 방어 효과에 중요한 IFN-γ, TNF-α 및 IL-2를 동시에 분비하는 다기능성 T세포 증가를 유도하는 효과가 있어 결핵 예방용 백신으로 유용하게 이용될 수 있으며, 나아가, 상기 당화된 Ag85A 단백질은 단백질 생산에 최적화된 벡터를 이용하여 식물체에서 효과적으로 발현되고 높은 수율로 분리될 수 있어 저렴한 비용으로 대량 생산이 가능하다.
PCT/KR2020/001230 2019-01-28 2020-01-23 당화된 ag85a 단백질을 포함하는 결핵 예방용 백신 조성물 및 이의 제조방법 WO2020159169A2 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP20748794.3A EP3919075A4 (en) 2019-01-28 2020-01-23 VACCINE COMPOSITION FOR THE PREVENTION OF TUBERCULOSIS WITH GLYCOSYLATED AG85A PROTEIN AND METHOD OF MANUFACTURE THEREOF
US17/426,275 US20220088167A1 (en) 2019-01-28 2020-01-23 Vaccine composition for preventing tuberculosis, comprising glycosylated ag85a protein and method for preparing same
JP2021543360A JP7233662B2 (ja) 2019-01-28 2020-01-23 グリコシル化したａｇ８５ａタンパク質を含む結核予防用ワクチン組成物およびその製造方法
CN202080011317.9A CN113365655A (zh) 2019-01-28 2020-01-23 包含糖基化ag85a蛋白的用于预防结核病的疫苗组合物及其制备方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2019-0010431 2019-01-28
KR1020190010431A KR102317403B1 (ko) 2019-01-28 2019-01-28 당화된 Ag85A 단백질을 포함하는 결핵 예방용 백신 조성물 및 이의 제조방법

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WO2020159169A2 WO2020159169A2 (ko) 2020-08-06
WO2020159169A3 true WO2020159169A3 (ko) 2020-10-15

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US (1) US20220088167A1 (ko)
EP (1) EP3919075A4 (ko)
JP (1) JP7233662B2 (ko)
KR (1) KR102317403B1 (ko)
CN (1) CN113365655A (ko)
WO (1) WO2020159169A2 (ko)

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KR102557824B1 (ko) * 2020-08-18 2023-07-20 주식회사 바이오앱 식물 발현 재조합 지카바이러스 외피단백질을 포함하는 백신 조성물 및 이의 제조방법
KR102630105B1 (ko) * 2020-11-26 2024-01-29 전남대학교산학협력단 Cel12A 단백질을 포함하는 셀룰로오스 분해용 조성물 및 이의 제조방법
KR20230119865A (ko) * 2022-02-08 2023-08-16 연세대학교 산학협력단 당사슬이 절단된 신규 항체를 유효성분으로 포함하는 암의 예방 또는 치료용 조성물

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Publication number Publication date
KR20200093723A (ko) 2020-08-06
EP3919075A4 (en) 2022-11-09
WO2020159169A2 (ko) 2020-08-06
EP3919075A2 (en) 2021-12-08
CN113365655A (zh) 2021-09-07
US20220088167A1 (en) 2022-03-24
JP2022518807A (ja) 2022-03-16
KR102317403B1 (ko) 2021-10-29
JP7233662B2 (ja) 2023-03-07

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