WO2018190677A3 - Method for purifying analogous antibody using cation-exchange chromatography - Google Patents
Method for purifying analogous antibody using cation-exchange chromatography Download PDFInfo
- Publication number
- WO2018190677A3 WO2018190677A3 PCT/KR2018/004345 KR2018004345W WO2018190677A3 WO 2018190677 A3 WO2018190677 A3 WO 2018190677A3 KR 2018004345 W KR2018004345 W KR 2018004345W WO 2018190677 A3 WO2018190677 A3 WO 2018190677A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cation
- exchange chromatography
- purifying
- analogous
- analogous antibody
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
- B01D15/426—Specific type of solvent
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
- C07K2317/522—CH1 domain
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2318/00—Antibody mimetics or scaffolds
Abstract
The present disclosure relates to a method for separating impurities of analogous antibodies using cation-exchange chromatography, and more specifically, to a method for separating the single fragments and isoforms of analogous antibodies, which are produced during the process of producing analogous antibodies, and purifying only target antibody fragments in high purity and yield using an elution buffer for the cation-exchange chromatography.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2017-0048635 | 2017-04-14 | ||
KR20170048635 | 2017-04-14 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2018190677A2 WO2018190677A2 (en) | 2018-10-18 |
WO2018190677A3 true WO2018190677A3 (en) | 2019-01-17 |
Family
ID=63792713
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2018/004345 WO2018190677A2 (en) | 2017-04-14 | 2018-04-13 | Method for purifying analogous antibody using cation-exchange chromatography |
Country Status (4)
Country | Link |
---|---|
KR (1) | KR102140693B1 (en) |
AR (1) | AR111465A1 (en) |
TW (1) | TWI679209B (en) |
WO (1) | WO2018190677A2 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102209790B1 (en) * | 2018-12-20 | 2021-02-01 | 에이치케이이노엔 주식회사 | Method for purification of vaccine virus using affinity chromatography |
CN110066314B (en) * | 2019-04-01 | 2023-10-27 | 上海药明生物技术有限公司 | Affinity purification process for efficiently improving separation resolution of multimer |
CN114539417A (en) * | 2020-11-26 | 2022-05-27 | 盛禾(中国)生物制药有限公司 | Chromatographic purification process for effectively removing bispecific antibody homodimers |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20130136883A (en) * | 2012-06-05 | 2013-12-13 | 씨제이제일제당 (주) | A novel method for purifying long-acting human growth hormone |
KR20140142974A (en) * | 2013-06-05 | 2014-12-15 | 한화케미칼 주식회사 | A Method of Antibody Purification |
US20150056196A1 (en) * | 2007-10-30 | 2015-02-26 | Genentech, Inc. | Antibody purification by cation exchange chromatography |
US20160264618A1 (en) * | 2013-10-30 | 2016-09-15 | Celltrion Inc. | Method for separating antibody isoforms using cation exchange chromatography |
US20170066814A1 (en) * | 2010-12-21 | 2017-03-09 | Hoffmann-La Roche Inc. | Isoform enriched antibody preparation and method for obtaining it |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5914342B2 (en) * | 2009-10-20 | 2016-05-11 | アッヴィ・インコーポレイテッド | Isolation and purification of anti-IL-13 antibody using protein A affinity chromatography |
-
2018
- 2018-04-13 TW TW107112953A patent/TWI679209B/en active
- 2018-04-13 AR ARP180100937A patent/AR111465A1/en unknown
- 2018-04-13 WO PCT/KR2018/004345 patent/WO2018190677A2/en active Application Filing
- 2018-04-13 KR KR1020180043341A patent/KR102140693B1/en active IP Right Grant
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150056196A1 (en) * | 2007-10-30 | 2015-02-26 | Genentech, Inc. | Antibody purification by cation exchange chromatography |
US20170066814A1 (en) * | 2010-12-21 | 2017-03-09 | Hoffmann-La Roche Inc. | Isoform enriched antibody preparation and method for obtaining it |
KR20130136883A (en) * | 2012-06-05 | 2013-12-13 | 씨제이제일제당 (주) | A novel method for purifying long-acting human growth hormone |
KR20140142974A (en) * | 2013-06-05 | 2014-12-15 | 한화케미칼 주식회사 | A Method of Antibody Purification |
US20160264618A1 (en) * | 2013-10-30 | 2016-09-15 | Celltrion Inc. | Method for separating antibody isoforms using cation exchange chromatography |
Non-Patent Citations (1)
Title |
---|
LEE, HONG JAI ET AL.: "Purification of antibody fragments for the reduction of charge variants using cation exchange chromatography", JOURNAL OF CHROMATOGRAPHY B, vol. 1080, 31 January 2018 (2018-01-31), pages 20 - 26, XP055566960 * |
Also Published As
Publication number | Publication date |
---|---|
TWI679209B (en) | 2019-12-11 |
AR111465A1 (en) | 2019-07-17 |
KR102140693B1 (en) | 2020-08-05 |
TW201841933A (en) | 2018-12-01 |
KR20180116159A (en) | 2018-10-24 |
WO2018190677A2 (en) | 2018-10-18 |
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