AR111465A1 - METHOD FOR PURIFICATION OF ANALOG ANTIBODIES BY THE USE OF CATION EXCHANGE CHROMATOGRAPHY - Google Patents
METHOD FOR PURIFICATION OF ANALOG ANTIBODIES BY THE USE OF CATION EXCHANGE CHROMATOGRAPHYInfo
- Publication number
- AR111465A1 AR111465A1 ARP180100937A ARP180100937A AR111465A1 AR 111465 A1 AR111465 A1 AR 111465A1 AR P180100937 A ARP180100937 A AR P180100937A AR P180100937 A ARP180100937 A AR P180100937A AR 111465 A1 AR111465 A1 AR 111465A1
- Authority
- AR
- Argentina
- Prior art keywords
- cation exchange
- exchange chromatography
- purification
- antibodies
- analog antibodies
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
- B01D15/426—Specific type of solvent
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
- C07K2317/522—CH1 domain
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2318/00—Antibody mimetics or scaffolds
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Peptides Or Proteins (AREA)
Abstract
Un método para la separación de impurezas de anticuerpos análogos, por el uso de una cromatografía de intercambio de cationes, y de manera más especifica, a un método para la separación de los fragmentos individuales e isoformas de anticuerpos análogos, que se producen durante el proceso de producción de anticuerpos análogos, y la purificación de solamente fragmentos de anticuerpos de destino en alta pureza y rendimiento por el uso de un tampón de elución para la cromatografía de intercambio de cationes.A method for the separation of impurities from analogous antibodies, by the use of a cation exchange chromatography, and more specifically, to a method for the separation of individual fragments and isoforms of analogous antibodies, which occur during the process of production of analogous antibodies, and the purification of only target antibody fragments in high purity and yield by the use of an elution buffer for cation exchange chromatography.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR20170048635 | 2017-04-14 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR111465A1 true AR111465A1 (en) | 2019-07-17 |
Family
ID=63792713
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP180100937A AR111465A1 (en) | 2017-04-14 | 2018-04-13 | METHOD FOR PURIFICATION OF ANALOG ANTIBODIES BY THE USE OF CATION EXCHANGE CHROMATOGRAPHY |
Country Status (4)
Country | Link |
---|---|
KR (1) | KR102140693B1 (en) |
AR (1) | AR111465A1 (en) |
TW (1) | TWI679209B (en) |
WO (1) | WO2018190677A2 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102209790B1 (en) * | 2018-12-20 | 2021-02-01 | 에이치케이이노엔 주식회사 | Method for purification of vaccine virus using affinity chromatography |
CN110066314B (en) * | 2019-04-01 | 2023-10-27 | 上海药明生物技术有限公司 | Affinity purification process for efficiently improving separation resolution of multimer |
CN114539417A (en) * | 2020-11-26 | 2022-05-27 | 盛禾(中国)生物制药有限公司 | Chromatographic purification process for effectively removing bispecific antibody homodimers |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BRPI0817182A2 (en) * | 2007-10-30 | 2015-03-17 | Genentech Inc | Method for purifying an antibody and compositions |
DK3037104T3 (en) * | 2009-10-20 | 2020-07-20 | Abbvie Inc | ISOLATION AND PURIFICATION OF ANTI-IL-13 ANTIBODIES USING PROTEIN A RELATED CHROMATOGRAPHY |
JP5838221B2 (en) * | 2010-12-21 | 2016-01-06 | エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト | Isoform-enriched antibody preparation and method for obtaining the same |
KR101460266B1 (en) * | 2012-06-05 | 2014-11-11 | 씨제이헬스케어 주식회사 | A novel method for purifying long-acting human growth hormone |
KR101569783B1 (en) * | 2013-06-05 | 2015-11-19 | 한화케미칼 주식회사 | A Method of Antibody Purification |
US10351592B2 (en) * | 2013-10-30 | 2019-07-16 | Celltrion, Inc. | Method for separating antibody isoforms using cation exchange chromatography |
-
2018
- 2018-04-13 WO PCT/KR2018/004345 patent/WO2018190677A2/en active Application Filing
- 2018-04-13 AR ARP180100937A patent/AR111465A1/en unknown
- 2018-04-13 KR KR1020180043341A patent/KR102140693B1/en active IP Right Grant
- 2018-04-13 TW TW107112953A patent/TWI679209B/en active
Also Published As
Publication number | Publication date |
---|---|
TWI679209B (en) | 2019-12-11 |
WO2018190677A2 (en) | 2018-10-18 |
KR20180116159A (en) | 2018-10-24 |
KR102140693B1 (en) | 2020-08-05 |
WO2018190677A3 (en) | 2019-01-17 |
TW201841933A (en) | 2018-12-01 |
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