WO2012173344A3 - Ｃｓｐ－ｂ ５'-ｓａｒ 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법 - Google Patents

Ｃｓｐ－ｂ ５'-ｓａｒ 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법 Download PDF

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Publication number
WO2012173344A3
WO2012173344A3 PCT/KR2012/003995 KR2012003995W WO2012173344A3 WO 2012173344 A3 WO2012173344 A3 WO 2012173344A3 KR 2012003995 W KR2012003995 W KR 2012003995W WO 2012173344 A3 WO2012173344 A3 WO 2012173344A3
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recombinant proteins
animal cells
same
vector
producing recombinant
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PCT/KR2012/003995
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English (en)
French (fr)
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WO2012173344A2 (ko
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고여욱
이상용
김수연
문승기
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주식회사종근당
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Priority to JP2014515713A priority Critical patent/JP5795434B2/ja
Priority to US14/125,705 priority patent/US9228218B2/en
Priority to EP12800088.2A priority patent/EP2719764B1/en
Priority to CN201280029037.6A priority patent/CN103797123B/zh
Priority to ES12800088.2T priority patent/ES2611402T3/es
Publication of WO2012173344A2 publication Critical patent/WO2012173344A2/ko
Publication of WO2012173344A3 publication Critical patent/WO2012173344A3/ko

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6408—Serine endopeptidases (3.4.21)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian

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  • Health & Medical Sciences (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 (a) CSP-B(Cytotoxic Serine Protease-B)의 5'-SAR(Scaffold or Matrix Attachment Region); (b) 동물세포에서 작동하는(operable) 프로모터; 및 (c) 폴리아데닐화 서열을 포함하는 동물세포용 발현 벡터, 그리고 이를 이용하여 재조합 단백질을 제조하는 방법에 관한 것이다. 본 발명의 벡터는 CSP-B 5'-SAR을 포함하는 벡터로, 동물세포에 도입한 외래 유전자의 위치에 따른 유전자 발현 억제 현상을 극복하는 효과를 가지며, 목적 단백질의 발현율을 크게 향상시킨다. 본 발명의 벡터는 의약품용 재조합 단백질 또는 항체를 동물세포에서 효과적으로 발현시킨다. 본 발명의 벡터 및 이를 이용한 재조합 단백질의 제조방법은 의약품의 산업적 대량 생산에 매우 유용하게 사용될 수 있다.
PCT/KR2012/003995 2011-06-13 2012-05-21 Ｃｓｐ－ｂ ５'-ｓａｒ 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법 WO2012173344A2 (ko)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2014515713A JP5795434B2 (ja) 2011-06-13 2012-05-21 Ｃｓｐ−ｂ５’−ｓａｒ因子を含む動物細胞発現ベクター、及びそれを利用した組換えタンパク質の製造方法
US14/125,705 US9228218B2 (en) 2011-06-13 2012-05-21 Expression vector for animal cells including CSP-B 5′-SAR factor and method for producing recombinant proteins using same
EP12800088.2A EP2719764B1 (en) 2011-06-13 2012-05-21 Expression vector for animal cells including csp-b 5'-sar factor and method for producing recombinant proteins using same
CN201280029037.6A CN103797123B (zh) 2011-06-13 2012-05-21 包含csp-b 5’-sar 因子的动物细胞表达载体及利用该载体的重组蛋白质的制备方法
ES12800088.2T ES2611402T3 (es) 2011-06-13 2012-05-21 Vector de expresión para células animales incluyendo el factor 5'-SAR de CSP-B y método para producir proteínas recombinantes mediante su utilización

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0056685 2011-06-13
KR1020110056685A KR101420274B1 (ko) 2011-06-13 2011-06-13 Ｃｓｐ－ｂ ５＇ｓａｒ 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법

Publications (2)

Publication Number Publication Date
WO2012173344A2 WO2012173344A2 (ko) 2012-12-20
WO2012173344A3 true WO2012173344A3 (ko) 2013-03-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/003995 WO2012173344A2 (ko) 2011-06-13 2012-05-21 Ｃｓｐ－ｂ ５'-ｓａｒ 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법

Country Status (7)

Country Link
US (1) US9228218B2 (ko)
EP (1) EP2719764B1 (ko)
JP (1) JP5795434B2 (ko)
KR (1) KR101420274B1 (ko)
CN (1) CN103797123B (ko)
ES (1) ES2611402T3 (ko)
WO (1) WO2012173344A2 (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9062326B2 (en) * 2011-03-30 2015-06-23 Pangen Biotech Inc. Expression vector for animal cells
EP2992104B1 (en) * 2013-05-03 2019-04-17 Fujifilm Diosynth Biotechnologies UK Limited Expression process
KR101599138B1 (ko) * 2014-06-17 2016-03-03 한국생명공학연구원 재조합 단백질 발현 증진을 위한 유전자 절편을 포함하는 벡터 및 이의 용도
KR101757346B1 (ko) 2017-03-27 2017-07-26 아주대학교산학협력단 항-emap ii 항체 및 이의 용도
US11479612B2 (en) 2017-05-30 2022-10-25 Chong Kun Dang Pharmaceutical Corp. Anti-c-Met antibody and use thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020010327A (ko) * 2000-07-29 2002-02-04 허영섭 동물세포 발현벡터
KR20020047006A (ko) * 2000-12-15 2002-06-21 리송알렉스인근 인터페론 베타 ｍａｒ 인자를 포함하는 동물세포 발현벡터
KR20070118563A (ko) * 2005-03-04 2007-12-17 주식회사 셀트리온 １ 카피 이상의 ｍａｒ ｄｎａ 서열이 유전자의전사종결영역의 3′말단에 삽입된 동물세포 발현 벡터 및그를 이용한 외래 유전자의 발현 방법

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AU2002216443A1 (en) * 2000-12-15 2002-06-24 Pangen Biotech Inc. Expression vector for animal cell containing nuclear matrix attachment region fointerferon beta
EP2097538A4 (en) * 2006-12-07 2011-11-30 Switchgear Genomics TRANSCRIPTION REAGULATION ELEMENTS OF BIOLOGICAL PATHS, TOOLS AND METHODS

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
KR20020010327A (ko) * 2000-07-29 2002-02-04 허영섭 동물세포 발현벡터
KR20020047006A (ko) * 2000-12-15 2002-06-21 리송알렉스인근 인터페론 베타 ｍａｒ 인자를 포함하는 동물세포 발현벡터
KR20070118563A (ko) * 2005-03-04 2007-12-17 주식회사 셀트리온 １ 카피 이상의 ｍａｒ ｄｎａ 서열이 유전자의전사종결영역의 3′말단에 삽입된 동물세포 발현 벡터 및그를 이용한 외래 유전자의 발현 방법

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ERIC M. THOMPSON ET AL.: "Scaffold Attachment Regions Stimulate HSP70.1 Expression in Mouse Preimplantation Embryos but Not in Differentiated Tissues", MOLECULAR AND CELLULAR BIOLOGY, vol. 14, July 1994 (1994-07-01), pages 4694 - 4703, XP055137957 *
ROBIN D. HANSON ET AL.: "A-T-rich scaffold attachment regions flank the hematopoietic serine protease genes clustered on chromosome 14ql 1.2", BLOOD, vol. 79, no. 3, 1 February 1992 (1992-02-01), pages 610 - 618, XP055137959 *
See also references of EP2719764A4 *

Also Published As

Publication number Publication date
KR101420274B1 (ko) 2014-07-21
CN103797123B (zh) 2016-04-20
CN103797123A (zh) 2014-05-14
ES2611402T3 (es) 2017-05-08
EP2719764A4 (en) 2014-12-24
WO2012173344A2 (ko) 2012-12-20
EP2719764A2 (en) 2014-04-16
KR20120137683A (ko) 2012-12-24
JP5795434B2 (ja) 2015-10-14
EP2719764B1 (en) 2016-10-26
US20140093914A1 (en) 2014-04-03
JP2014516580A (ja) 2014-07-17
US9228218B2 (en) 2016-01-05

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