Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
  • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
  • C07D487/04—Ortho-condensed systems
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
  • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
  • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/53—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with three nitrogens as the only ring hetero atoms, e.g. chlorazanil, melamine
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
  • A61P35/02—Antineoplastic agents specific for leukemia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P37/00—Drugs for immunological or allergic disorders
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D519/00—Heterocyclic compounds containing more than one system of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring system not provided for in groups C07D453/00 or C07D455/00

Definitions

• the present invention relates to pyrazolo[l,5-a][l,3,5]triazine and pyrazolo[l,5-a]pyrimidine derivatives and/or pharmaceutically acceptable salts thereof, the use of these derivatives as pharmaceutically active agents, especially for the prophylaxis and/or treatment of cell proliferative diseases, inflammatory diseases, immunological diseases, cardiovascular diseases and infectious diseases. Furthermore, the present invention is directed towards pharmaceutical compositions containing at least one of the pyrazolo[l,5-a][l,3,5]triazine and pyrazolo[ 1,5 -a] pyrimidine derivatives and/or pharmaceutically acceptable salts thereof.
• CDK family members that trigger passage through the cell cycle are being considered as attractive therapeutic targets, especially for cancer.
• CDK family members that control other processes such as transcription and RNA processing have caught less attention so far, although experimental evidence for their involvement in different pathological processes is emerging.
• CDK/cyclin complexes also have been identified as conserved components of the RNA polymerase II (Pol II) transcriptional machinery ( Bregman et ah, 2000, Front Biosci. 5:244-257).
• RNA polymerase II RNA polymerase II
• CDK7 has consolidated kinase activities, regulating both the cell cycle progression and transcription ( Desai et ah, 1995, Mol. Cell Biol. 15, 345-350).
• the general transcription factor TFIIH purified from mammalian cells consists of ten subunits, seven of which (p62, p52, p44, p34, XPD, XPB, and TTDA) form the core complex. Three subunits (cyclin H, MAT1, and CDK7) from the CDK-activating kinase (CAK), which is linked to TFIIH’s core via the XPD (ATP-dependent helicase) subunit of complex.
• CAK CDK-activating kinase
• CDK7 phosphorylates the C-terminal domain (CTD) of Pol II at serine 5 and 7 (Akhtar et ah, 2009, Mol. Cell 34, 387-393) as well as other transcription factors controlling the initiation-to-elongation transition (Larochelle et al., 2012, Nat. Strut. Mol. Biol. 19, 1108- 1115 Therefore CDK7 is essential factor for transcription process, which suggests that CDK7 is a target for cancer therapy, especially transcription addicted cancer. CDK7 has long been asserted as having an essential role in cellular metabolism and viability.
• Transcriptional CDK inhibitors down-regulate a large number of short-lived anti-apoptotic proteins, such as the anti-apoptotic proteins myeloid cell leukemia-l (Mcl-l), B-cell lymphoma extra-long (Bcl-xL) and XIAP (X-linked IAP), D-cyclins, c-Myc, Mdm-2 (leading to p53 stabilization), p2l wafl proteins whose transcription is mediated by nuclear factor-kappa B (NF-kB) and hypoxia-induced VEGF ⁇ Shapiro GL 2006, J Clin Oncol; 24(11): 1770-83).
• Mcl-l myeloid cell leukemia-l
• Bcl-xL B-cell lymphoma extra-long
• XIAP X-linked IAP
• D-cyclins c-Myc
• Mdm-2 leading to p53 stabilization
• p2l wafl proteins whose
• CDK7 as regulator of general transcription and CDK7 is a therapeutic target for treatment of many diseases and syndromes are associated with mutations in regulatory regions and in transcription factors, cofactors, chromatin regulators and noncoding RNAs. These mutations can contribute to cancer, autoimmunity, neurological disorders, developmental syndromes, diabetes, cardiovascular disease, and obesity, among others. Some transcription factors control RNA polymerase II pause release and elongation and, when their expression or function is altered, can produce aggressive tumor cells (c-Myc) or some forms of autoimmunity (AIRE) ⁇ Tong Ihn Lee and Richard A. Young, Cell, 2013, 152:1237-1251).
• c-Myc c-Myc
• AIRE autoimmunity
• CDK7 is a therapeutic target for treatment of disease like inflammation, virus replication such as HIV, EBV, cancer and cardiac hypertrophy.
• HIV-l gene expression is regulatory by a viral transactivator protein (Tat) which induces transcriptional elongation of HIV-l long tandem repeat. This induction requires hyperphosphorylation of the C-terminal domain repeat of RNA polymerase II.
• Tat stimulates CTD kinases associated with general transcription factors of the promoter complex, specifically TFIIH-associated CDK7 (Nekhai et al.; Biochem J. (2002) 364, 649-657).
• the inventors of US 615968 also described that Tat binds to CDK7 and that this interaction increase the ability of CAK to phosphorylate CTD.
• the authors of US 615968 further disclose that the transcriptional activation by Tat is dependent upon the kinase activity of CDK7. Additionally, Young Kyeung Kim and colleagues conclude that the recruitment and activation of TFIIH represents a rate-limiting step for the emergence of HIV from latency (Young Kyeung Kim, EMBO (2006) 25, 3596-3604).
• MAT- 1/cyclin H are upregulated during Human cytomegalovirus infection.
• kinase activities of CDK7 and CDK9 Troprakar et al., Journal of Virology, 2005, 79; 15477-15493.
• antiviral drugs target viral proteins. These have the disadvantage that viruses often develop resistance against these drugs. Antiviral drugs targeting cellular proteins essential for viral process, like CDK7, could bypass this disadvantage. These drugs may further be effective in treating several unrelated viruses and their effects should be additive to traditional antiviral agents. Inhibitors of CDK7, which has its dual function of CDK-activating kinase and transcription regulation is very effective in the treatment of several viruses.
• the present invention relates to pyrazolo-triazine or pyrazolo-pyrimidine compounds which are defined by general formula I
• X is, independently at each occurrence, selected from CH and N;
• Q is, independently at each occurrence, selected from the group consisting of C3-C8 cycloalkyl, aryl, heteroaryl, heterocyclyl, and C1-C6 alkyl, wherein C1-C6 alkyl is substituted with one or two of OR 5 , -N(R 5 )R 5 , aryl, heteroaryl and heterocyclyl,
• C3-C8 cycloalkyl can be substituted with one or two of R 3 and R 4 and -(CO)R 5
• heterocyclyl can be substituted with one or two of R 3 and R 4 and -(CO)R 5 aryl or heteroaryl substituted with one or two of C1-C6 alkyl, -OR 5 , -N(R 5 )R 5 , - (CO)R 5 , halogen, heteroaryl and heterocyclyl;
• R 1 is, at each occurrence, independently selected from the group consisting of hydrogen and methyl
• R 5 is, at each occurrence, independently selected from the group consisting of hydrogen, Cl- C6 alkyl, C3-C8 cycloalkyl, C1-C3 haloalkyl, heteroaryl, heterocyclyl, heteroaryl substituted with one or two of halogen, -OR 11 , -N(R 11 )R 11 , C1-C6 alkyl and C1-C6 alkyl substituted with -OH or -NH 2 , heterocyclyl substituted with one or two of halogen, -OR 11 , -N(R u )R n , C1-C6 alkyl and C1-C6 alkyl substituted with -OH or -NH 2 ;
• Z is any structure of the following group A;
• X is, independently at each occurrence, selected from CR and N;
• X 2 is, independently at each occurrence, selected from CR 25 and N;
• R 9 is, at each occurrence, independently selected from the group consisting of hydrogen, halogen, C1-C6 alkyl, C1-C3 haloalkyl, -OR 5 , -CN, C3-C10 cycloalkyl, C3-C10 heterocyclyl and C1-C6 alkyl substituted with -OH or -OR 5 ;
• R 11 is, at each occurrence, independently selected from the group consisting of hydrogen, Cl- C6 alkyl and C3-C10 cycloalkyl;
• R° is, at each occurrence, independently selected from the group consisting of hydrogen, Cl- C6 alkyl, C1-C6 alkyl substituted with -CN, -OH, -OR 5 , -NH 2 , -NHR 5 or -N(R 5 )R 5 and C3- C10 cycloalkyl;
• R 17 , R 18 , R 19 and R 20 are, at each occurrence, independently selected from the group consisting of hydrogen, halogen, C1-C6 alkyl, C1-C3 haloalkyl, C6-C10 aryl, e.g.
• R 21 is, at each occurrence, independently selected from the group consisting of C1-C6 alkyl, C3-C10 cycloalkyl, C3-C10 heterocyclyl, C1-C3 haloalkyl, aryl, phenyl, benzyl, C1-C6 alkyl substituted with -CN, -OH, -OR 5 , -NH 2 , -NHR 5 or -N(R 5 )R 5 , aryl substituted with halogen or C1-C3 haloalkyl, C3-C10 heteroaryl substituted with one to four halogens or C1-C3 alkyl and C3-C10 heterocyclyl substituted with R 4 ;
• R 22 and R 23 are, at each occurrence, independently selected from the group consisting of hydrogen, halogen, C1-C6 alkyl, C1-C3 haloalkyl, -OH, -OR 5 , -CN and C1-C6 alkyl substituted with -OH, -OR 5 or -NHR 9 ;
• R 24 and R 25 are, at each occurrence, independently selected from the group consisting of
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is absent
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is N(R 5 )R 5
• R 4 is H
• R 5 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is CHs
• R 4 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 6 is not CH 3 , Cl or lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is CH 3
• R 4 is H
• X is N
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 12 is not phenyl, CH(CH 3 ) 2 , CH 2 CH 3 or CH 3 ;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is C3 ⁇ 4
• R 4 is H
• X is N
• Z is phenyl
• R 6 is Cl
• R 7 is H
• R 8 is H and R 9 is H
• R 10 is not Cl
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is CH 3
• R 4 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is Cl
• R 6 is not Cl
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is CHs
• R 4 is H
• X is N
• Z is phenyl
• R 6 is Cl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is not C3 ⁇ 4;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is CH 3
• R 4 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is Cl
• R 6 is not CH 3 ;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is CH 3
• R 4 is H
• X is N
• Z is phenyl
• R 6 is F
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is not F
• R 1 is H
• R 2 is CH(CFI 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is CH 3
• R 4 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is F
• R 6 is not F;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is CH 3
• R 4 is H
• X is N
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 12 is not CH 3 ;
• R 1 is H
• R 2 is CH(CFI 3 )2
• Q is heterocyclyl substituted with R 3 and R 4
• R 4 is H
• X is N
• Z is phenyl
• R 6 is lH-pyrazole
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 3 is not H;
• R 1 is FI
• R 2 is CH(CH 3 ) 2
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is N(R 5 )R 5
• R 4 is H
• R 5 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is H
• R 4 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H
• R 6 is not lH- pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is 0,
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is H
• R 4 is H
• X is N
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H
• R 12 is not CH(CH 3 ) 2 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is H
• R 4 is H
• X is N
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 12 is not CH 3 ;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is C3-C8 cycloalkyl substituted with R 3 and R 4
• R 3 is N(R 5 )R 5
• R 4 is H
• R 5 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is NH
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is H
• R 4 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 6 is not 1H- pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is NH
• Q is C3-C8 cycloalkyl substituted with R 3 and R 4
• R 3 is N(R 5 )R 5
• R 4 is H
• R 5 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is H
• R 4 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is F
• R 6 is not 1H- pyrazole
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is H
• R 4 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 6 is not 1H- pyr azole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is CH 3
• R 4 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H
• R 6 is not lH- pyrazole
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is H
• R 4 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H and R 10 is H
• then R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is CH 3
• R 4 is H
• X is N
• Z is phenyl
• R 6 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 7 is not Cl
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is CH 3
• R 4 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H
• R 10 is H
• R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q is C3-C8 cycloalkyl substituted with R 3 and R 4
• R 3 is N(R 5 )R 5
• R 4 is H
• R 5 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H
• R 10 is H, then R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is NH
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is H
• R 4 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H and R 10 is H
• R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is absent
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is N(R 5 )R 5
• R 4 is H
• R 5 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H
• R 10 is H
• R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is absent
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is N(R 5 )R 5
• R 4 is H
• R 5 is H
• X is N
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is NH
• Q is C3-C8 cycloalkyl substituted with R 3 and R 4
• R 3 is N(R 5 )R 5
• R 4 is H
• R 5 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H and R 10 is H, then R 12 is not C3 ⁇ 4
• R 1 is C3 ⁇ 4
• R 2 is CH(CH 3 ) 2
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is H
• R 4 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H and R 10 is H, then R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• Q is heterocyclyl substituted with R 3 and R 4
• R 3 is N(R S )R 5
• R 4 is H
• R 5 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H and R 10 is H, then R 12 is not CH 3 ; or an enantiomer, stereoisomeric form, mixture of enantiomers, diastereomer, mixture of diastereomers, racemate of the above mentioned compounds, or a pharmaceutically acceptable salt thereof.
• R 1 is hydrogen and the compound has the general formula II
• the present invention relates to compounds having the general formula III
• Q 1 is either absent or independently, at each occurrence, selected from the group consisting of aryl, heteroaryl, heterocyclyl, aryl substituted with one or two of C1-C6 alkyl, -OR 5 , - N(R 5 )R 5 , and halogen; heteroaryl substituted with one or two of C1-C6 alkyl, -OR 5 , -N(R 5 )R 5 and halogen; and heterocyclyl substituted with one or two of R and R ;
• R 5 , R 9 and R 12 are as defined in claim 1 ;
• Y 1 is, independently at each occurrence, selected from CH, C(OH) and N;
• Y is, independently at each occurrence, selected from CH, CR , O andN;
• m is, independently at each occurrence, selected from 0, 1 and 2;
• n is, independently at each occurrence, selected from 0 and 1;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is absent
• Q 1 is absent
• L 2 is absent
• Y 1 is N
• Y 2 is CH
• m is 1
• n is 1
• R 29 is N(R 5 )R 5
• R 30 is H
• R 5 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H, then R 6 is not lH-pyrazole;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n 1
• R 29 is C3 ⁇ 4
• R 30 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n 1
• R 29 is C3 ⁇ 4
• R 30 is H
• X is N
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 12 is not phenyl, CH(CH 3 ) 2 , CH 2 CH 3 or CH 3 ;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n is 1
• R 29 is CH 3
• R 30 is H
• X is N
• Z is phenyl
• R 6 is Cl
• R 7 is H
• R 8 is H
• R 9 is H, then R 10 is not Cl;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n is 1
• R 29 is CH 3
• R 30 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is Cl
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n is 1
• R 29 is C3 ⁇ 4
• R 30 is H
• X is N
• Z is phenyl
• R 6 is Cl
• R 7 is H
• R 8 is H
• R 9 is H
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n 1
• R 29 is C3 ⁇ 4
• R 30 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is Cl
• R 1 is H, R 2 is CH(CH 3 ) , L 1 is O, Q 1 is absent, L 2 is absent, Y 1 is CH, Y 2 is N, m is 1, n is 1, R 29 is CH 3 , R 30 is H, X is N, Z is phenyl, R 6 is F, R 7 is H, R 8 is H and R 9 is H, then R 10 is not F;
• R 1 is H, R 2 is CH(CH 3 )2, L 1 is O, Q 1 is absent, L 2 is absent, Y 1 is CH, Y 2 is N, m is 1, n is 1, R 29 is CH 3 , R 30 is H, X is N, Z is phenyl, R 7 is H, R 8 is H, R 9 is H and R 10 is F, then R 6 is not F;
• R 1 is FI
• R 2 is CH(CH 3 ) 2
• Q 1 is absent
• L 2 is absent
• Y 1 is N
• Y 2 is N
• m is 1
• n is 1
• R 30 is H
• X is N
• Z is phenyl
• R 6 is lH-pyrazole
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H, then R 29 is not H;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• Q 1 is absent
• L 2 is absent
• Y 1 is N
• Y 2 is CH
• m is 1
• n is 1
• R 29 is N(R 5 )R 5
• R 30 is H
• R 5 is H
• R 30 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H, then R 6 is not IH-pyrazole;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n is 1
• R 29 is H
• R 30 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H, then R 6 is not lH-pyrazole;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n is 1
• R 29 is H
• R 30 is H
• X is N
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H, then R 12 is not CH(CH 3 ) 2 ;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n 0,
• R 29 is H
• R 30 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H, then R 6 is not lH-pyrazole;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n 0,
• R 29 is H
• R 30 is H
• X is N
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H, then R 12 is not CH(CH 3 ) 2 ;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is CH
• m 1, n is 1
• R 29 is N(R 5 )R 5
• R 30 is H
• R 5 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 H and R 10 is H
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is NH
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m 1, n is 0,
• R 29 is H
• R 30 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H, then R is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is absent
• Q 1 is absent
• L 2 is absent
• Y 1 is N
• Y 2 is CH
• m is 1
• n is 0,
• R 29 is N(R 5 )R 5
• R 30 is H
• R 5 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH ) 2
• L 1 is NH
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is CH
• m is 1
• n is 1
• R 29 is N(R 5 )R 5
• R 30 is H
• R 5 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is absent
• Q 1 is absent
• L 2 is absent
• Y 1 is N
• Y 2 is CH
• m 2
• n is 0,
• R 29 is N(R 5 )R 5
• R 30 is H
• R 5 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 2
• n is 0,
• R 29 is H
• R 30 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is F
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n is 1
• R 29 is C3 ⁇ 4
• R 30 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 1 is C3 ⁇ 4, R 2 is CH(CH 3 ) 2 , L 1 is O, Q 1 is absent, L 2 is absent, Y 1 is CH, Y 2 is N, m is 1, n is 1, R 29 is CH 3 , R 30 is H, X is N, Z is phenyl, R 6 is OR 12 , R 7 is H, R 8 is H, R 9 is H and R 10 is H, then R 12 is not CH 3 ;
• R 1 is CH 3 , R 2 is CH(CH 3 ) 2 , L 1 is O, Q 1 is absent, L 2 is absent, Y 1 is CH, Y 2 is N, m is 1, n is 0, R 29 is H, R 30 is H, X is N, Z is phenyl, R 6 is OR 12 , R 7 is H, R 8 is H, R 9 is H and R 10 is H, then R 12 is not C3 ⁇ 4;
• R 1 is CH 3 , R 2 is CH(CH 3 ) 2 , L 1 is O, Q 1 is absent, L 2 is absent, Y 1 is CH, Y 2 is N, m is 1, n is 1, R 29 is H, R 30 is H, X is N, Z is phenyl, R 6 is OR 12 , R 7 is H, R 8 is H, R 9 is H and R 10 is H, then R 12 is not C3 ⁇ 4;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 2
• n is 0,
• R 29 is H
• R 30 is H
• X is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H, then R 6 is not lH-pyr azole;
• R 1 is C3 ⁇ 4
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n is 1
• R 29 is H
• R 30 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H and R 10 is H, then R 12 is not C3 ⁇ 4;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n is 1
• R 29 is CH 3
• R 30 is H
• X is N
• Z is phenyl
• R 6 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n 0,
• R 29 is H
• R 30 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H
• R 10 is H, then R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• m is 1
• n is 1
• R 29 is CH 3
• R 30 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H and R 10 is H, then R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is O
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is CH
• m is 1
• n is 1
• R 29 is N(R 5 )R 5
• R 30 is H
• R 5 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H and R 10 is H, then R 12 is not C3 ⁇ 4;
• R 1 is CH 3 , R 2 is CH(CH 3 ) 2 , L 1 is NH, Q 1 is absent, L 2 is absent, Y 1 is CH, Y 2 is N, m is 1, n is 0, R 29 is H, R 30 is H, X is N, Z is phenyl, R 6 is H, R 7 is OR 12 , R 8 is H, R 9 is H and R 10 is H, then R 12 is ot CH 3 ;
• R 1 is CH 3 , R 2 is CH(CH 3 ) 2 , L 1 is absent, Q 1 is absent, L 2 is absent, Y 1 is N, Y 2 is CH, m is 1, n is 0, R 29 is N(R 5 )R 5 , R 30 is H, R 5 is H, X is N, Z is phenyl, R 6 is H, R 7 is OR 12 , R 8 is H, R 9 is H and R 10 is H, then R 12 is not C3 ⁇ 4;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is absent
• Q 1 is absent
• L 2 is absent
• Y 1 is N
• Y 2 is CH
• m is 1
• n is 1
• R 29 is N(R 5 )R 5
• R 30 is H
• R 5 is H
• X is N
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H, then R 12 is not CH 3 ;
• R 1 is C3 ⁇ 4
• R 2 is CH(CH 3 ) 2
• L 1 is absent
• Q 1 is absent
• L 2 is absent
• Y 1 is N
• Y 2 is CH
• m is 1
• n is 1
• R 29 is N(R 5 )R 5
• R 30 is H
• R 5 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H and R 10 is H, then R 12 is not C3 ⁇ 4;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is NH
• Q 1 is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is CH
• m is 1
• n is 1
• R 29 is N(R 5 )R 5
• R 30 is H
• R 5 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H and R 10 is H, then R 12 is not C3 ⁇ 4;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• Q 1 is absent
• L 2 is absent
• Y 1 is N
• Y 2 is N
• m is 1
• n is 1
• R 29 is H
• R 30 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H and R 10 is H, then R 12 is not C3 ⁇ 4;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• Q 1 is absent
• L 2 is absent
• Y 1 is N
• Y 2 is CH
• m is 1
• n is 1
• R 29 is N(R 5 )R 5
• R 30 is H
• R 5 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H and R 10 is H, then R 12 is not C3 ⁇ 4;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• L 1 is absent
• Q 1 is absent
• L 2 is absent
• Y 1 is N
• Y 2 is CH
• m 2
• n is 0,
• R 29 is N(R 5 )R 5
• R 30 is H
• R 5 is H
• X is N
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H and R 10 is H, then R 12 is not CH 3 ; or an enantiomer, stereoisomeric form, mixture of enantiomers, diastereomer, mixture of diastereomers, racemate of the above mentioned compounds, or a pharmaceutically acceptable salt thereof.
• the present invention relates to compounds having the general formula la
• X is, independently at each occurrence, selected from CH and N;
• Y 1 is, independently at each occurrence, selected from CH, C(OH) andN;
• Y 2 is, independently at each occurrence, selected from CH, CR 4 , O and N; m is, independently at each occurrence, selected from 0, 1 and 2;
• n is, independently at each occurrence, selected from 0 and 1;
• Q is either absent or independently, at each occurrence, selected from the group consisting of heterocyclyl, C3-C6 heteroaryl, aryl, e.g. phenyl, aryl substituted with halogen;
• R 1 is, at each occurrence, independently selected from the group consisting of hydrogen and methyl
• R 5 is, at each occurrence, independently selected from the group consisting of C1-C6 alkyl, C3-C6 cycloalkyl, C3-C10 heterocyclyl, C1-C3 haloalkyl and C3-C10 heterocyclyl substituted with halogen, -OH, -N3 ⁇ 4, C1-C6 alkyl and C1-C6 alkyl substituted with -OH or - NH 2 ;
• Z is any structure of the following group A;
• X 1 is, independently at each occurrence, selected from CR 24 and N;
• X 2 is, independently at each occurrence, selected from CR 25 and N;
• R 9 is, at each occurrence, independently selected from the group consisting of hydrogen, halogen, C1-C6 alkyl, C1-C3 haloalkyl, -OR 5 , -CN, C3-C10 cycloalkyl, C3-C10 heterocyclyl and C1-C6 alkyl substituted with -OH or -OR 5 ;
• R 11 is, at each occurrence, independently selected from the group consisting of hydrogen, Cl- C6 alkyl and C3-C10 cycloalkyl;
• R 13 is, at each occurrence, independently selected from the group consisting of hydrogen, Cl- C6 alkyl, C1-C6 alkyl substituted with -CN, -OH, -OR 5 , -NH 2 , -NHR 5 or -N(R 5 )R 5 and C3- C10 cycloalkyl;
• R 17 , R 18 , R 19 and R 20 are, at each occurrence, independently selected from the group consisting of hydrogen, halogen, C1-C6 alkyl, C1-C3 haloalkyl, C6-C10 aryl, e.g.
• R 21 is, at each occurrence, independently selected from the group consisting of C1-C6 alkyl, C3-C10 cycloalkyl, C3-C10 heterocyclyl, C1-C3 haloalkyl, aryl, phenyl, benzyl, C1-C6 alkyl substituted with -CN, -OH, -OR 5 , -NH 2 , -NHR 5 or -N(R 5 )R 5 , aryl substituted with halogen or C1-C3 haloalkyl, C3-C 10 heteroaryl substituted with one to four halogens or C1-C3 alkyl and C3-C10 heterocyclyl substituted with R 4 ;
• R 22 and R 23 are, at each occurrence, independently selected from the group consisting of hydrogen, halogen, C1-C6 alkyl, C1-C3 haloalkyl, -OH, -OR 5 , -CN and C1-C6 alkyl substituted with -OH, -OR 5 or -NHR 9 ;
• R 24 and R 25 are, at each occurrence, independently selected from the group consisting of 7
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is N3 ⁇ 4
• R 4 is H
• L 1 is absent
• Q is absent
• L 2 is absent
• X is N
• n is 1
• Y 1 is N
• Y 2 is CH
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is CH 3
• R 4 is H
• L 1 is O
• Q is absent
• L 2 is absent
• X is N
• n is 1
• Y 1 is CH
• Y 2 is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H, then R 6 is not CH 3 , Cl or lH-pyrazole;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is CH 3
• R 4 is H
• L 1 is O
• Q is absent
• L 2 is absent
• X is N
• n is 1
• Y 1 is CH
• Y 2 is N
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H, then R 12 is not phenyl, CH(CH 3 ) 2 , CH 2 CH 3 or CH 3 ;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is CH 3
• R 4 is H
• L 1 is O
• Q is absent
• L 2 is absent
• X is N
• n is 1
• Y 1 is CH
• Y 2 is N
• Z is phenyl
• R 6 is Cl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is not Cl
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is CH 3
• R 4 is H
• L 1 is O
• Q is absent
• L 2 is absent
• X is N
• n is 1
• Y 1 is CH
• Y 2 is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is Cl
• R 6 is not Cl
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is C3 ⁇ 4
• R 4 is H
• L 1 is O
• Q is absent
• L 2 is absent
• X is N
• n is 1
• Y 1 is CH
• Y 2 is N
• Z is phenyl
• R 6 is Cl
• R 7 is H
• R 8 is H and R 9 is H
• R 10 is not CH 3
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is CH 3
• R 4 is H
• L 1 is O
• Q is absent
• L 2 is absent
• X is N
• n is 1 is 1, Y 1 is CH
• Y 2 is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is Cl
• R 6 is not CH 3 ;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is CH 3
• R 4 is H
• L 1 is O
• Q is absent
• L 2 is absent
• X is N
• n is 1
• Y 1 is CH
• Y 2 is N
• Z is phenyl
• R 6 is F
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is not F
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is CH 3
• R 4 is H
• L 1 is O
• Q is absent
• L 2 is absent
• X is N
• n is 1
• Y 1 is CH
• Y 2 is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is F, then R 6 is not F;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• X is N
• L 1 is O
• Q is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• n is 1
• R 3 is C3 ⁇ 4
• R 4 is H
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H, then R 12 is not C3 ⁇ 4;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• X is N
• Q is absent
• L 2 is absent
• Y 1 is N
• Y 2 is N
• n is 1
• R 3 is H
• R 4 is H
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H, then R 6 is not lH-pyr azole;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• X is N
• Q is absent
• L 2 is absent
• Y 1 is N
• Y 2 is CH
• n is 1
• R 3 is N3 ⁇ 4
• R 4 is H
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 1 is H
• R 2 is CH(CH 3 ) 2
• X is N
• L 1 is O
• Q is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• n is 1
• R 3 is H
• R 4 is H
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• X is N
• L 1 is O
• Q is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• n is 1
• R 3 is H
• R 4 is H
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H, then R 12 is not CH(CH 3 ) 2
• R 1 is H
• R 2 is CH(CH 3 ) 2
• X is N
• L 1 is O
• Q is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• n is 0,
• R 3 is H
• R 4 is H
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H, then R 6 is not lH-pyr azole;
• R 1 is H
• R 2 is CH(CH 3 )
• X is N
• L 1 is O
• Q is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• n is 0,
• R 3 is H
• R 4 is H
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H, then R 12 is not CH(CH 3 ) 2 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• X is N
• L 1 is O
• Q is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• n is 0,
• R 3 is H
• R 4 is H
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H, then R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH ) 2
• X is N
• L 1 is O
• Q is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• n is 1
• R 3 is H
• R 4 is H
• Z is phenyl
• R 6 is OR 12
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H, then R 12 is not CH 3 ;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is N3 ⁇ 4
• R 4 is H
• L 1 is O
• Q is absent
• L 2 is absent
• X is N
• n is 1
• Y 1 is CH
• Y 2 is CH
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is H
• R 4 is H
• L 1 is NH
• Q is absent
• L 2 is absent
• X is N
• n is 0,
• Y 1 is CH
• Y 2 is N
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is N3 ⁇ 4
• R 4 is H
• L 1 is absent
• Q is absent
• L 2 is absent
• X is N
• n is 0,
• Y 1 is N
• Y 2 is CH
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H
• R 10 is H, then R 6 is not lH-pyrazole;
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is N3 ⁇ 4
• R 4 is H
• L 1 is NH
• Q is absent
• L 2 is absent
• X is N
• n is 1
• Y 1 is CH
• Y 2 is CH
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H
• R 6 is not lH-pyrazole
• R 1 is H
• R 2 is CH(CH 3 ) 2
• R 3 is H
• R 4 is NH 2
• L 1 is absent
• Q is absent
• L 2 is absent
• X is N
• n is 1
• Y 2 is CH
• Z is phenyl
• R 7 is H
• R 8 is H
• R 9 is H and R 10 is H
• R 6 is not lH-pyrazole
• R 1 is CH 3
• R 2 is CH(CH3) 2
• X is N
• L 1 is O
• Q is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• n 1
• R 3 is H
• R 4 is H
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H
• R 10 is H, then R 12 is not CH 3 ;
• R 1 is C3 ⁇ 4, R 2 is CH(CH 3 ) 2 , X is N, L 1 is O, Q is absent, L 2 is absent, Y 1 is CH, Y 2 is N, n is 1, R 3 is CH 3 , R 4 is H, Z is phenyl, R 6 is H, R 8 is H, R 9 is H and R 10 is H, then R 7 is not Cl;
• R 1 is C3 ⁇ 4, R 2 is CH(CH 3 ) 2 , X is N, L 1 is O, Q is absent, L 2 is absent, Y 1 is CH, Y 2 is N, n is 1, R 3 is H, R 4 is H, Z is phenyl, R 6 is H, R 7 is OR 12 , R 8 is H, R 9 is H and R 10 is H, then R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• X is N
• L 1 is O
• Q is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is N
• n 1
• R 3 is NH 2
• R 4 is H
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H
• R 10 is H
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• X is N
• L 1 is O
• Q is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is CH
• n 1
• R 3 is NH 2
• R 4 is H
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H
• R 10 is H
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• X is N
• L 1 is absent
• Q is absent
• L 2 is absent
• Y 1 is N
• Y 2 is CH
• n 1
• R 3 is NH 2
• R 4 is H
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H
• R 10 is H
• R 1 is CH 3 , R 2 is CH(CH 3 ) 2 , X is N, L 1 is NH, Q is absent, L 2 is absent, Y 1 is CH, Y 2 is N, n is 0, R 3 is H, R 4 is H, Z is phenyl, R 6 is H, R 7 is OR 12 , R 8 is H, R 9 is H and R 10 is H, then R 12 is not CH 3 ;
• R 1 is CH 3 , R 2 is CH(CH 3 )2
• X is N, L 1 is absent, Q is absent, L 2 is absent, Y 1 is N, Y 2 is CH, n is 0, R 3 is N3 ⁇ 4, R 4 is H, Z is phenyl, R 6 is H, R 7 is OR 12 , R 8 is H, R 9 is H and R 10 is H, then R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• X is N
• L 1 is NH
• Q is absent
• L 2 is absent
• Y 1 is CH
• Y 2 is CH
• n 1
• R 3 is N3 ⁇ 4
• R 4 is H
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H
• R 10 is H, then R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• X is N
• Q is absent
• L 2 is absent
• Y 1 is N
• Y 2 is N
• n 1
• R 3 is H
• R 4 is H
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H
• R 10 is H, then R 12 is not CH 3 ;
• R 1 is CH 3
• R 2 is CH(CH 3 ) 2
• X is N
• L 1 is absent
• Q is absent
• L 2 is absent
• Y 1 is N
• Y 2 is CH
• n 1
• R 3 is NH
• R 4 is N3 ⁇ 4
• Z is phenyl
• R 6 is H
• R 7 is OR 12
• R 8 is H
• R 9 is H
• R 10 is H, then R 12 is not CH 3 ;
• the present invention relates to compounds having the general formula IY
• Z 1 is any structure of the following group D;
• R 6 , R 7 , R 8 , R 9 and R 10 are as defined above for general formula I.
• the present invention relates to compounds having the general formula V
• the present invention relates to compounds having the general formula VI
• X 3 is, independently at each occurrence, selected from CR 10 and N;
• R , R and R are, at each occurrence, independently selected from the group consisting of hydrogen, halogen, C1-C6 alkyl, C1-C3 haloalkyl, -OR 5 , -CN and C1-C6 alkyl substituted with -OH, -OR 5 or -NHR 9 ;
• R s , R 9 and R 10 are as defined above for general formula I;
• R 7 is any structure of the following group E;
• R 8 and R 14 - R 20 are as defined above for general formula I.
• the present invention relates to compounds having the general formula VII
• the present invention relates to compounds having the general formula VIII
• the present invention relates to compounds having the general formula IX
• Q 2 is any structure of the following group F;
• the present invention also relates to pharmaceutically acceptable salts of the compounds according to the present invention, as defined herein.
• the compound according to the present invention is a compound selected from structures 1 - 279, as listed further below in table 11.
• the present invention relates to a pharmaceutical composition
• a pharmaceutical composition comprising a compound according to the present invention as defined herein, as an active ingredient, together with at least one pharmaceutically acceptable carrier, excipient and/or diluent.
• the present invention also relates to a compound according to the present invention as defined herein, for use as a pharmaceutical or pharmaceutically active agent, wherein said pharmaceutical or pharmaceutically active agent preferably has an inhibitory activity on cyclin-dependent kinase 7 (CDK7).
• CDK7 cyclin-dependent kinase 7
• the present invention also relates to a compound according to the present invention, as defined herein, for use in a method of prevention and/or treatment of a disease which is associated with inhibition of apoptosis, abnormal transcriptional activity and/or cell cycle arrest by aberrant activity and/or overexpression of one or several cyclin-dependent kinases (CDKs), in particular cyclin-dependent kinase 7 (CDK7), wherein the disease is selected from proliferative diseases, infectious diseases, including opportunistic diseases, immunological diseases, autoimmune diseases, and inflammatory diseases.
• CDKs cyclin-dependent kinases
• CDK7 cyclin-dependent kinase 7
• the disease associated with inhibition of apoptosis, abnormal transcriptional activity and/or cell cycle arrest by aberrant activity and/or overexpression of one or several cyclin-dependent kinases (CDKs), in particular cyclin-dependent kinase 7 (CDK7) is a disease associated with, accompanied by, caused by and/or induced by CDK7 dysfunction and/or hyperfunction.
• the disease associated with inhibition of apoptosis, abnormal transcriptional activity and/or cell cycle arrest by aberrant activity and/or overexpression of one or several cyclin-dependent kinases (CDKs), in particular cyclin-dependent kinase 7 (CDK7) is a proliferative disease.
• said proliferative disease is a cancer.
• said cancer is selected from adenocarcinoma, choroidal melanoma, acute leukemia, acoustic neurinoma, ampullary carcinoma, anal carcinoma, astrocytoma, basal cell carcinoma, pancreatic cancer, Desmoid tumor, bladder cancer, bronchial carcinoma, estrogen dependent and independent breast cancer, Burldtt’s lymphoma, corpus cancer, Carcinoma unknown primary tumor (CUP-syndrome), colorectal cancer, small intestine cancer, small intestinal tumors, ovarian cancer, endometrial carcinoma, ependymoma, epithelial cancer types, Ewing’s tumors, gastrointestinal tumors, gastric cancer, gallbladder cancer, gall bladder carcinomas, uterine cancer, cervical cancer, cervix, glioblastomas, gynecologic tumors, ear, nose and throat tumors, hematologic tumor, hairy cell leukemia, urethral cancer, skin cancer
• said infectious disease including opportunistic diseases, is selected from AIDS, Adenovirus Infection, Alveolar Hydatid Disease (AHD), Amoebiasis, Angiostrongyliasis, Anisakiasis, Anthrax, Babesiosis, Balantidiasis, Baylisascaris Infection, Bilharzia (Schistosomiasis), Blastocystis hominis Infection, Lyme Borreliosis, Botulism, Brainerd Diarrhea, Brucellosis, Bovine Spongiform Encephalopathy (BSE), Candidiasis, Capillariasis, Chronic Fatigue Syndrome (CFS), Chagas Disease, Chickenpox, Chlamydia pneumoniae Infection, Cholera, Chronic Fatigue Syndrome, Creutzfeldt- Jakob Disease (CJD), Clonorchiasis, Cutaneous Larva migrans (CLM), Coccidioidomycosis
• the immunological disease and/or autoimmune disease is selected from asthma, diabetes, rheumatic diseases, rejection of transplanted organs and tissues, rhinitis, chronic obstructive pulmonary diseases, osteoporosis, ulcerative colitis, sinusitis, lupus erythematosus, recurrent infections, atopic dermatitis / eczema and occupational allergies, food allergies, drug allergies, severe anaphylactic reactions, anaphylaxis, manifestations of allergic diseases, primary immunodeficiencies, antibody deficiency states, cell mediated immunodeficiencies, severe combined immunodeficiency, DiGeorge syndrome, Hyper IgE syndrome (HIES), Wiskott-Aldrich syndrome (WAS), ataxia-telangiectasia, immune mediated cancers, white cell defects, autoimmune diseases, systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), multiple sclerosis (MS), immune-mediated or Type
• the inflammatory disease is caused, induced, initiated and/or enhanced by bacteria, viruses, prions, parasites, fungi, and/or caused by irritative, traumatic, metabolic, allergic, autoimmune, or idiopathic agents.
• the inflammatory disease is selected from the group comprising or consisting of inflammatory diseases of the central nervous system (CNS), inflammatory rheumatic diseases, inflammatory diseases of blood vessels, inflammatory diseases of the middle ear, inflammatory bowel diseases, inflammatory diseases of the skin, inflammatory disease uveitis, and inflammatory diseases of the larynx.
• CNS central nervous system
• inflammatory rheumatic diseases inflammatory diseases of blood vessels
• inflammatory diseases of the middle ear inflammatory diseases of the middle ear
• inflammatory bowel diseases inflammatory diseases of the skin
• inflammatory disease uveitis inflammatory diseases of the larynx.
• the inflammatory disease is selected from inflammatory diseases of the central nervous system (CNS), inflammatory rheumatic diseases, inflammatory diseases of blood vessels, inflammatory diseases of the middle ear, inflammatory bowel diseases, inflammatory diseases of the skin, inflammatory disease uveitis, inflammatory diseases of the larynx, wherein preferably said inflammatory diseases are selected from the group comprising abscessation, acanthamoeba infection, acne vulgaris, actinomycosis, acute inflammatory dermatoses, acute laryngeal infections of adults, acute multifocal placoid pigment epitheliopathy, acute (thermal) injury, acute retinal necrosis, acute suppurative otitis media, algal disorders, allergic contact dermatitis, amyloidosis angioedema, ankylosing spondylitis, aspergillosis, atopic dermatitis, pseudorabies, autoantibodies in vasculitis, bacterial disorders, bacterial laryngit
• the present invention also relates to a method of treatment and/or prevention of a disease which is associated with inhibition of apoptosis, abnormal transcriptional activity and/or cell cycle arrest by aberrant activity and/or overexpression of one or several cyclin-dependent kinases (CDKs), in particular cyclin-dependent kinase 7 (CDK7), wherein the disease is selected from proliferative diseases, infectious diseases, including opportunistic diseases, immunological diseases, autoimmune diseases, and inflammatory diseases, wherein said method of treatment and/or prevention comprises administering a compound according to the present invention as defined herein, to a patient in need thereof.
• CDKs cyclin-dependent kinases
• CDK7 cyclin-dependent kinase 7
• the patient in need thereof is a mammal. In one embodiment, the patient in need thereof is a human being. In another embodiment, the patient in need thereof is a nonhuman animal.
• the disease which is prevented or treated in said method is as defined herein.
• the present invention also relates to the use of a compound according to the present invention as defined herein in the manufacture of a medicament for the prevention and/or treatment of a disease which is associated with inhibition of apoptosis, abnormal transcriptional activity and/or cell cycle arrest by aberrant activity and/or overexpression of one or several cyclin-dependent kinases (CDKs), in particular cyclin-dependent kinase 7 (CDK7), wherein the disease is selected from proliferative diseases, infectious diseases, including opportunistic diseases, immunological diseases, autoimmune diseases, and inflammatory diseases, as defined herein.
• CDKs cyclin-dependent kinases
• CDK7 cyclin-dependent kinase 7
• the compounds of the present invention are highly efficient inhibitors of CDK7 threonine/serine kinase and/or its complex, CDK7/MATl/CycH.
• the inventive compounds are suitable for the use as a pharmaceutically active agent.
• the inventive compounds are suitable for the treatment of disorders associated with, accompanied by, caused by and/or induced by CDK7 and its complex, in particular a hyperfunction or dysfunction thereof.
• the inventive compounds are thus suitable for the treatment of CDK7-associated diseases or disorders and CDK7 complex induced disorders.
• inventive compounds are also useful in the manufacture of a medicament or of a pharmaceutical composition for the treatment of disorders associated with, accompanied by, caused by and/or induced by CDK7 and its complex, in particular a hyperfunction or dysfunction thereof.
• inventive compounds are further used in the manufacture of a medicament or of a pharmaceutical composition for the treatment and/or prevention of CDK7 and its complex induced disorders.
• halogen including fluorine, C ! -C 3 alkyl, C1-C3 haloalkyl, methylhydroxyl, COOMe, C(0)H, COOH, OMe, or OCF 3 ;
• alkyl refers to a monovalent straight, branched or cyclic chain, saturated aliphatic hydrocarbon radical having a number of carbon atoms in the specified range.
• “C C 6 alkyl” refers to any of the hexyl alkyl and pentyl alkyl isomers as well as n-, iso-, sec-, and t-butyl, n- and isopropyl, cyclic propyl, ethyl and methyl.
• alkenyl refers to a monovalent straight or branched chain aliphatic hydrocarbon radical containing one carbon-carbon double bond and having a number of carbon atoms in the specified range.
• “C 2 -C 6 alkenyl” refers to all of the hexenyl and pentenyl isomers as well as 1-butenyl, 2-butenyl, 3-butenyl, isobutenyl, l-propenyl, 2- propenyl, and ethenyl (or vinyl).
• cycloalkyl refers to a group, such as optionally substituted or non-substituted cyclic hydrocarbon, having from three to eight carbon atoms, unless otherwise defined.
• “C 3 -Cs cycloalkyl” refers to cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl.
• haloalkyl refers to an alkyl group, as defined herein that is substituted with at least one halogen.
• straight or branched chained“haloalkyl” groups useful in the present invention include, but are not limited to, methyl, ethyl, propyl, isopropyl, «-butyl, and /-butyl substituted independently with one or more halogens.
• the term“haloalkyl” should be interpreted to include such substituents such as -CHF 2 , -CF 3 , -CH 2 -CH 2 -F, -CH 2 -CF 3 , and the like.
• heteroalkyl refers to an alkyl group where one or more carbon atoms have been replaced with a heteroatom, such as, O, N, or S.
• a heteroatom e.g., O, N, or S
• the resulting heteroalkyl groups are, respectively, an alkoxy group (e.g., -OCTl 3 , etc.), an amine (e.g, -NFICH 3 , -N(CH 3 ) 2 , etc.), or thioalkyl group (e.g., -SC3 ⁇ 4, etc.).
• a non-terminal carbon atom of the alkyl group which is not attached to the parent molecule is replaced with a heteroatom (e.g., O, N, or S) and the resulting heteroalkyl groups are, respectively, an alkyl ether (e.g., -CH CH 2 -0-CH 3 , etc.), alkyl amine (e.g., -CH 2 NHCF1 3 , -CFI 2 N(CH3) 2 , etc.), or thioalkyl ether (e.g., -CH 2 -S-CH 3 ).
• an alkyl ether e.g., -CH CH 2 -0-CH 3 , etc.
• alkyl amine e.g., -CH 2 NHCF1 3 , -CFI 2 N(CH3) 2 , etc.
• thioalkyl ether e.g., -CH 2 -S-CH 3
• halogen refers to fluorine, chlorine, bromine, or iodine.
• phenyl as used herein is meant to indicate that optionally substituted or non- substituted phenyl group.
• heteroaryl refers to (i) optionally substituted 5- and 6-membered heteroaromatic rings and (ii) optionally substituted 9- and 10-membered bicyclic, fused ring systems in which at least one ring is aromatic, wherein the heteroaromatic ring or the bicyclic, fused ring system contains from 1 to 4 heteroatoms independently selected from N, O, and S, where each N is optionally in the form of an oxide and each S in a ring which is not aromatic is optionally S(O) or S(0) 2 .
• Suitable 5- and 6-membered heteroaromatic rings include, for example, pyridyl, pyrrolyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, thienyl, furanyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isooxazolyl, oxadiazolyl, thiazolyl, isothiazolyl, and thiadiazolyl.
• Suitable 9-and lO-membered heterobicyclic, fused ring systems include, for example, benzofuranyl, indolyl, indazolyl, naphthyridinyl, isobenzofuranyl, benzopiperidinyl, benzisoxazolyl, benzoxazolyl, chromenyl, quinolinyl, isoquinolinyl, cinnolinyl, quinazolinyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, isoindolyl, benzodioxolyl, benzofuranyl, imidazo[l,2-a]pyridinyl, benzotriazolyl, dihydroindolyl, dihydroisoindolyl, indazolyl, indolinyl, isoindolinyl, quinoxalinyl, quinazolinyl, 2,3- dihydr
• heterocyclyl refers to (i) optionally substituted 4- to 8-membered, saturated and unsaturated but non-aromatic monocyclic rings containing at least one carbon atom and from 1 to 4 heteroatoms, (ii) optionally substituted bicyclic ring systems containing from 1 to 6 heteroatoms, and (iii) optionally substituted tricyclic ring systems, wherein each ring in (ii) or (iii) is independent of fused to, or bridged with the other ring or rings and each ring is saturated or unsaturated but nonaromatic, and wherein each heteroatom in (i), (ii), and (iii) is independently selected from N, O, and S, wherein each N is optionally in the form of an oxide and each S is optionally oxidized to S(O) or S(0) 2 .
• Suitable 4- to 8-membered saturated heterocyclyls include, for example, azetidinyl, piperidinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, isothiazolidinyl, oxazolidinyl, isoxazolidinyl, pyrrolidinyl, imidazolidinyl, piperazinyl, tetrahydrofuranyl, tetrahydrothienyl, pyrazolidinyl, hexahydropyrimidinyl, thiazinanyl, thiazepanyl, azepanyl, diazepanyl, tetrahydropyranyl, tetrahydrothiopyranyl, dioxanyl, and azacyclooctyl.
• Suitable unsaturated heterocyclic rings include those corresponding to the saturated heterocyclic rings listed in the above sentence in which a single bond is replaced with a double bond. It is understood that the specific rings and ring systems suitable for use in the present invention are not limited to those listed in this and the preceding paragraphs. These rings and ring systems are merely representative.
• Examples of pharmaceutically acceptable addition salts include, without limitation, the nontoxic inorganic and organic acid addition salts such as the acetate derived from acetic acid, the aconate derived from aconitic acid, the ascorbate derived from ascorbic acid, the benzenesulfonate derived from benzensulfonic acid, the benzoate derived from benzoic acid, the cinnamate derived from cinnamic acid, the citrate derived from citric acid, the embonate derived from embonic acid, the enantate derived from enanthic acid, the formate derived from formic acid, the fumarate derived from fumaric acid, the glutamate derived from glutamic acid, the glycolate derived from glycolic acid, the hydrochloride derived from hydrochloric acid, the hydrobromide derived from hydrobromic acid, the lactate derived from lactic acid, the maleate derived from maleic acid, the malonate derived from malonic
• acids such as oxalic acid, which may not be considered pharmaceutically acceptable, may be useful in the preparation of salts useful as intermediates in obtaining a chemical compound of the invention and its pharmaceutically acceptable acid addition salt.
• the compounds of the invention are used in their respective free base form according to the present invention.
• Metal salts of a chemical compound of the invention include alkali metal salts, such as the sodium salt of a chemical compound of the invention containing a carboxy group.
• the chemical compounds of the invention may be provided in unsolvated or solvated forms together with a pharmaceutically acceptable solvent(s) such as water, ethanol, and the like.
• Solvated forms may also include hydrated forms such as the monohydrate, the dihydrate, the hemihydrate, the trihydrate, the tetrahydrate, and the like. In general, solvated forms are considered equivalent to unsolvated forms for the purposes of this invention.
• Table 10 shows comparative data of a panel assay showing the CDK7 selectivity profile in CDKs family for compound 210 of the invention.
• Table 11 summarizes compounds 1-279 in terms of their structures and corresponding characteristics.
• Figure 1 show in vivo antitumor activity of CDK7 inhibitor in OVCAR-3 xenograft model.
• Example 1 Enzymatic assay for CDKL CDK2, CDK5 and CDK7
• CDKl/Cyclin B Invitrogen, Cat# PR4768C
• 22 uM for CDK2/Cyclin A Invitrogen, Cat# PV6290
• 10 uM for CDK5/p25 Invitrogen, Cat# PR8543B
• 400 uM for CDK7/Cyclin H/MNAT1 Invitrogen, Cat# PR6749B
• Example 2 Cellular HCT116. H460, MV4-11. MM.1S. MOLT-4. RPMI-8226. A2780 and OVCAR-3 viability assay
• Cells were grown in RPMI-1640 media (Invitrogen, Cat#22400-089) supplemented with 10% FBS (Invitrogen, Cat# 10099141) and 1% penicillin/streptomycin (Invitrogen, Cat# 15070063) and cultured at 37 ° C , 5%C0 2 in a humidified chamber. All cell lines were routinely tested for mycoplasma.
• Cell HCT116, H460, MV4-11, MM.1S, MOLT-4, RPMI-8226, A2780 and OVCAR-3 viability assay protocol The effect of the CDK7 inhibitor to inhibit the growth of target cancer cells was evaluated through the 72 hours time period of viability assay. Briefly, the candidate cell line ware plated in 96 well plate at the following density of cells respectively. 1 X 10 4 cells/well for MOLT-4, RPMI-8226, MV4-11 and MM.1S, 5 X 10 3 for H460, HCT116 and OVCAR-3 and IX l0 3 for A2780. After 24 hours, the cells were treated with various concentrations of the compound (ranging from 0.0015uM to lOuM).
• DMSO solvent without compound served as a control and final DMSO concentration lest than 0.1%.
• DMSO concentration lest than 0.1%.
• cells were analyzed for the viability using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Cat# G7570). All viability assays were performed in duplicate and Luminescence was read using an Envision (Perkin Elmer, USA). Data was analyzed using XLfit software.
• the IC50 profile of compounds were determined using 28 CDKs family protein kinases through ProQinase GmbH (Freiburg, Germany). All the protocol and materials had been provided by ProQinase GmbH. Briefly, in the process, 90 m ⁇ H 2 0 were added to each well of a compound dilution plate. To minimize potential precipitation, the H 2 0 was added to the plate only a few minutes before the transfer of the compound solutions into the assay plates. The plate was shaken thoroughly, resulting in a "compound dilution plate/ 10 % DMSO". The compound dilution plate(s) were discarded at the end of the working day.
• the purity of the protein kinases was examined by SDS-PAGE/Coomassie staining, the identity was checked by mass spectroscopy.
• Kinases from external vendors, Cama Biosciences Inc.; Invitrogen Corp.; and Millipore Corp. were expressed, purified and quality-controlled by virtue of the vendors readings.
• a radiometric protein kinase assay 33 PanQinase ® Activity Assay was used for measuring the kinase activity of the 28 protein kinases. All kinase assays were performed in 96-well FlashPlatesTM from PerkinElmer (Boston, MA, USA) in a 50 m ⁇ reaction volume. The reaction cocktails were incubated at 30 °C for 60 minutes. The reaction was stopped with 50 m ⁇ of 2 % (v/v)
• OVCAR-3 (ATCC, HTB-161) xenograft tumors
• OVCAR-3 cells were grown in RPMI-1 640 medium (Gibco, C2400500BT) supplemented with 20 % fetal bovine serum(HyCl one, SV30087.03), 0.01 mg/ml bovine insulin (Yuanyue, S12033) and 1 % anti anti(G ibco, 15240-062) at 37 ° C in an atmosphere of 5 % C0 2 in air.
• Route II Route II having the similar reaction condition with Route I and different order.
• Compound Cl 3 can be synthesized by treating in presence of m-CPBA.
• the compounds of formula II- 1 can be synthesized by treating compounds C13 with appropriated group A.
• Compounds II-2 can be synthesized by treating in presence of POCl 3 .
• the compounds of formula 1-3 can be synthesized by treating compounds II-2 with DIPEA and group B.
• the compounds of formula 1-4 can be prepared by using compounds of formula 1-3 in presence of acid such as TFA, HBr and AcOH, or base such as hydrazine.
• Compound El can be synthesized by treating in presence of NaOEt.
• Compound E2 can be synthesized by treating in presence of POCl 3 .
• Compound E2 can be further treated with group B in presence of K 2 C0 3 to give compounds III- 1.
• Compounds III-1 can be further treated with group A to provide the compounds of formula III-2.
• the compounds of formula III-3 can be prepared by using compounds of formula III-2 in presence of acid such as TFA, HBr and AcOH, or base such as hydrazine. Procedure for synthesis of El
• reagent for group A such as tert-butyl piperidin-3- ylcarbamate, tert-butyl 4-aminopiperidine-l-carboxylate, piperidine-4-carboxamide, tert- butyl (4-methylpiperidin-4-yl)carbamate, tert-butyl piperidin-4-ylcarbamate, benzyl piperidin-4-ylcarbamate, piperidin-4-ol, piperazine, piperazin-2-one, tetrahydro-2H-pyran-4- amine, tert-butyl 3-methylpiperazine-l-carboxylate, N-(piperidin-4-yl)acetamide, 1- methylpiperidin-4-ol, tert-butyl ((4-hydroxypiperidin-4-yl)methyl)carbamate, tert-butyl (morpholin-2-ylmethyl)carbamate, tert-butyl 4-(amino
• reaction mixture was quenched with NH 4 C1 (200 mL) and extracted with EA (150 mL x 2). The organic layer was dried over anhydrous Na 2 S0 4 , filtered and concentrated under reduced pressure to give crude product. The residue was purified by column chromatography to give compound B124 (1.3 g) as light yellow oil.
• the compound B140 (500 mg) was followed the same procedure of B26 to obtain 910 mg of compound B141 as a yellow powder.
• the compound B142 (100 mg) was followed the same procedure of B28 to obtain 100 mg of compound B 143 as a pale-yellow powder.
• the red solid was purified by cation exchange resin eluting with 5% NH 3 .H 2 0, then lyophilized to give a white powder, which was purified by prep-TLC and lyophilized to give compound 178 (20.5 mg) as a white powder.
• N- isopropylpropan-2-amine (13.7 g, 135 mmol) dropwise at -70 °C under N 2 atmosphere.
• the mixture was stirred at 15 °C for 1 hour under N 2 atmosphere.
• compound D9 (12.0 g, 123 mmol) in anhydrous THF (20 mL) was added into the mixture at -70 °C under N 2 atmosphere and the resulting mixture was stirred at -70 °C under N 2 atmosphere for 2 hours.

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