WO2019074333A3 - 티오레독신 1 에피토프 및 이에 특이적으로 결합하는 단일클론항체 - Google Patents

티오레독신 1 에피토프 및 이에 특이적으로 결합하는 단일클론항체 Download PDF

Info

Publication number
WO2019074333A3
WO2019074333A3 PCT/KR2018/012069 KR2018012069W WO2019074333A3 WO 2019074333 A3 WO2019074333 A3 WO 2019074333A3 KR 2018012069 W KR2018012069 W KR 2018012069W WO 2019074333 A3 WO2019074333 A3 WO 2019074333A3
Authority
WO
WIPO (PCT)
Prior art keywords
thioredoxin
epitope
antibody
trx1
present
Prior art date
Application number
PCT/KR2018/012069
Other languages
English (en)
French (fr)
Other versions
WO2019074333A2 (ko
WO2019074333A9 (ko
Inventor
서경훈
김대중
김용
김미경
정종환
이기세
Original Assignee
주식회사 이앤에스헬스케어
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to BR112020007326-1A priority Critical patent/BR112020007326A2/pt
Application filed by 주식회사 이앤에스헬스케어 filed Critical 주식회사 이앤에스헬스케어
Priority to AU2018349387A priority patent/AU2018349387B2/en
Priority to CN202310788407.4A priority patent/CN118005720A/zh
Priority to RU2020113284A priority patent/RU2797266C2/ru
Priority to EP18866509.5A priority patent/EP3708575A4/en
Priority to US16/755,035 priority patent/US11953502B2/en
Priority to CN202310788312.2A priority patent/CN116769039A/zh
Priority to JP2020520447A priority patent/JP6995197B2/ja
Priority to CN201880066028.1A priority patent/CN111315759B/zh
Publication of WO2019074333A2 publication Critical patent/WO2019074333A2/ko
Publication of WO2019074333A3 publication Critical patent/WO2019074333A3/ko
Publication of WO2019074333A9 publication Critical patent/WO2019074333A9/ko
Priority to US18/443,970 priority patent/US20240192215A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57415—Specifically defined cancers of breast
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/54—F(ab')2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90209—Oxidoreductases (1.) acting on NADH or NADPH (1.6), e.g. those with a heme protein as acceptor (1.6.2) (general), Cytochrome-b5 reductase (1.6.2.2) or NADPH-cytochrome P450 reductase (1.6.2.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 티오레독신 1(Trx1) 항원의 에피토프 및 이의 용도에 관한 것으로, 보다 상세하게는 상기 에피토프 및 이에 결합하는 항체 또는 항원 결합 단편을 제공한다. 본 발명에서 확인된 인간 Trx1 항원의 에피토프 부위는 항-Trx1 항체의 결합 친화도를 향상시키기 위한 개량 항체 개발에 유용하게 활용될 수 있다. 또한, 본 발명의 개량된 항체는 기존의 항-Trx1 항체 보다 티오레독신 1에 대한 결합 친화도가 우수하고, 민감도와 특이도가 매우 높아 유방암 진단키트의 성능을 개선하는데 유용하다. 나아가, 기존의 다른 유방암 진단 바이오마커인 CA15-3을 검출하는 것 보다 본 발명의 티오레독신 1에 특이적으로 결합하는 항체를 사용하여 티오레독신 1을 검출하는 것이 민감도와 특이도가 월등히 우수하여 유방암 진단의 정확성 및 신뢰성을 현저히 높일 수 있다.
PCT/KR2018/012069 2017-10-12 2018-10-12 티오레독신 1 에피토프 및 이에 특이적으로 결합하는 단일클론항체 WO2019074333A2 (ko)

Priority Applications (10)

Application Number Priority Date Filing Date Title
US16/755,035 US11953502B2 (en) 2017-10-12 2018-10-12 Thioredoxin 1 epitope, encoding nucleic acid and methods of binding a monoclonal antibody
AU2018349387A AU2018349387B2 (en) 2017-10-12 2018-10-12 Thioredoxin 1 epitope and monoclonal antibody specifically binding thereto
CN202310788407.4A CN118005720A (zh) 2017-10-12 2018-10-12 硫氧还蛋白1表位及与其特异性结合的单克隆抗体
RU2020113284A RU2797266C2 (ru) 2017-10-12 2018-10-12 Эпитоп тиоредоксина-1 и моноклональное антитело, специфически связывающееся с ним
EP18866509.5A EP3708575A4 (en) 2017-10-12 2018-10-12 THIOREDOXIN-1 EPITOPE AND SPECIFICALLY BONDING MONOCLONAL ANTIBODIES
BR112020007326-1A BR112020007326A2 (pt) 2017-10-12 2018-10-12 epítopo de tioredoxina-1 e anticorpo monoclonal especificamente ligante ao mesmo
CN202310788312.2A CN116769039A (zh) 2017-10-12 2018-10-12 硫氧还蛋白1表位及与其特异性结合的单克隆抗体
JP2020520447A JP6995197B2 (ja) 2017-10-12 2018-10-12 チオレドキシン１エピトープおよびこれに特異的に結合する単一クローン抗体
CN201880066028.1A CN111315759B (zh) 2017-10-12 2018-10-12 硫氧还蛋白1表位及与其特异性结合的单克隆抗体
US18/443,970 US20240192215A1 (en) 2017-10-12 2024-02-16 Thioredoxin 1 epitope and monoclonal antibody specifically binding thereto

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0132536 2017-10-12
KR20170132536 2017-10-12

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US16/755,035 A-371-Of-International US11953502B2 (en) 2017-10-12 2018-10-12 Thioredoxin 1 epitope, encoding nucleic acid and methods of binding a monoclonal antibody
US18/443,970 Continuation US20240192215A1 (en) 2017-10-12 2024-02-16 Thioredoxin 1 epitope and monoclonal antibody specifically binding thereto

Publications (3)

Publication Number Publication Date
WO2019074333A2 WO2019074333A2 (ko) 2019-04-18
WO2019074333A3 true WO2019074333A3 (ko) 2019-07-04
WO2019074333A9 WO2019074333A9 (ko) 2019-09-12

Family

ID=66101617

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/012069 WO2019074333A2 (ko) 2017-10-12 2018-10-12 티오레독신 1 에피토프 및 이에 특이적으로 결합하는 단일클론항체

Country Status (8)

Country Link
US (2) US11953502B2 (ko)
EP (1) EP3708575A4 (ko)
JP (1) JP6995197B2 (ko)
KR (1) KR102112690B1 (ko)
CN (3) CN111315759B (ko)
AU (1) AU2018349387B2 (ko)
BR (1) BR112020007326A2 (ko)
WO (1) WO2019074333A2 (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2018349387B2 (en) 2017-10-12 2022-05-19 E&S Healthcare Co., Ltd. Thioredoxin 1 epitope and monoclonal antibody specifically binding thereto
KR102330205B1 (ko) * 2019-02-13 2021-11-24 주식회사 베르티스 암의 진단용 조성물
CN111718416B (zh) * 2020-06-25 2022-04-01 菲鹏生物股份有限公司 抗硫氧还蛋白的抗体、其应用和诊断试剂盒
CN111574631B (zh) * 2020-06-25 2022-04-01 菲鹏生物股份有限公司 针对硫氧还蛋白的抗体、缀合物和检测试剂盒
CN111875702B (zh) * 2020-08-13 2021-12-28 中国人民解放军北部战区总医院 抗creg单克隆抗体及应用
CN114920845A (zh) * 2020-12-31 2022-08-19 中元汇吉生物技术股份有限公司 特异性结合人IgG4的蛋白及其应用
CN113999819B (zh) * 2021-11-10 2023-11-28 青岛硕景生物科技有限公司 分泌抗Trx蛋白单克隆抗体的杂交瘤细胞株、抗Trx蛋白单克隆抗体

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06261783A (ja) * 1993-03-16 1994-09-20 Toyota Central Res & Dev Lab Inc チオレドキシンに特異的に反応するモノクローナル抗体
KR20100104110A (ko) * 2009-03-16 2010-09-29 배재대학교 산학협력단 티오레독신 1을 유효성분으로 하는 유방암 진단용 마커, 및이를 이용한 유방암 진단 키트

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020102654A1 (en) 1998-06-30 2002-08-01 Incyte Pharmaceuticals, Inc Thioredoxin proteins
KR100836516B1 (ko) * 1998-12-17 2008-06-12 코릭사 코포레이션 난소암의 진행을 억제하기 위한 약제학적 조성물 및 백신
US6528054B1 (en) 1998-12-28 2003-03-04 Corixa Corporation Compositions and methods for the therapy and diagnosis of breast cancer
US20090075871A2 (en) * 2003-03-31 2009-03-19 National Institute Of Advanced Industrial Science And Technology Modified Thioredoxin
WO2005117930A2 (en) 2004-06-04 2005-12-15 Sloan-Kettering Institute For Cancer Research Use of thioredoxin measurements for diagnostics and treatments
CN101495628A (zh) * 2006-05-25 2009-07-29 惠氏公司 血管及炎性疾病中半胱氨酸蛋白酶豆荚蛋白的表达
EA201000809A1 (ru) * 2007-11-16 2010-12-30 Те Рокфеллер Юниверсити АНТИТЕЛА, СПЕЦИФИЧЕСКИЕ В ОТНОШЕНИИ ПРОТОФИБРИЛЛЯРНОЙ ФОРМЫ БЕЛКА β-АМИЛОИДА
US9382587B2 (en) 2009-03-16 2016-07-05 Paichai University Industry-Academic Cooperation Foundation Diagnosis of breast cancer based on expression level of thioredoxin-1
US8512963B2 (en) * 2009-11-25 2013-08-20 The Johns Hopkins University Detection and quantitation of full-length thioredoxin (TRX) and truncated thioredoxin (TRX 80) in complex samples
JP2013101923A (ja) 2011-10-21 2013-05-23 Semiconductor Energy Lab Co Ltd 分散組成物の加熱方法、及びガラスパターンの形成方法
KR101171716B1 (ko) * 2011-12-14 2012-08-09 배재대학교 산학협력단 티오레독신 １을 유효성분으로 하는 난소암 또는 폐렴 진단용 조성물 및 이의 이용
CN109863176B (zh) * 2016-09-13 2023-04-21 E&S医疗保健有限公司 与硫氧还蛋白1特异性结合的单克隆抗体及其用途
AU2018349387B2 (en) 2017-10-12 2022-05-19 E&S Healthcare Co., Ltd. Thioredoxin 1 epitope and monoclonal antibody specifically binding thereto

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06261783A (ja) * 1993-03-16 1994-09-20 Toyota Central Res & Dev Lab Inc チオレドキシンに特異的に反応するモノクローナル抗体
KR20100104110A (ko) * 2009-03-16 2010-09-29 배재대학교 산학협력단 티오레독신 1을 유효성분으로 하는 유방암 진단용 마커, 및이를 이용한 유방암 진단 키트

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE NCBI 14 January 2016 (2016-01-14), ANONYMOUS: "Immunoglobulin heavy chain, partial [Mus musculus)", XP055623479, retrieved from NCBI Database accession no. BAU30829 *
DATABASE PROTEIN 14 September 2001 (2001-09-14), ANONYMOUS: "Ig 6D12.Fl light chain, partial [Mus musculus)", XP055623483, retrieved from NCBI Database accession no. AAB05154 *
DATABASE PROTEIN 26 July 2016 (2016-07-26), ANONYMOUS: "Immunoglobulin light chain variable region, partial (Mus musculus)", XP055623478, retrieved from NCBI Database accession no. AAF88053 *
DATABASE PROTEIN 9 March 1994 (1994-03-09), ANONYMOUS: "Immunoglobulin heavy chain, partial [Mus musculus", XP055623486, retrieved from NCBI Database accession no. AAA16583 *
PARK, B. J.: "Thioredoxin 1 as a serum marker for breast cancer and its use in combination with CEA or CA 15-3 for improving the sensitivity of breast cancer diagnoses", BMC RESEARCH NOTES, vol. 7, no. 7, 6 January 2014 (2014-01-06), pages 1 - 12, XP021173712 *

Also Published As

Publication number Publication date
KR20190041431A (ko) 2019-04-22
AU2018349387B2 (en) 2022-05-19
RU2020113284A (ru) 2021-11-12
RU2020113284A3 (ko) 2021-11-12
CN111315759A (zh) 2020-06-19
WO2019074333A2 (ko) 2019-04-18
CN116769039A (zh) 2023-09-19
RU2022106116A (ru) 2022-04-25
US20240192215A1 (en) 2024-06-13
EP3708575A2 (en) 2020-09-16
CN118005720A (zh) 2024-05-10
BR112020007326A2 (pt) 2020-09-29
US20200309778A1 (en) 2020-10-01
KR102112690B1 (ko) 2020-05-19
JP6995197B2 (ja) 2022-02-04
US11953502B2 (en) 2024-04-09
WO2019074333A9 (ko) 2019-09-12
AU2018349387A1 (en) 2020-04-30
JP2020536555A (ja) 2020-12-17
CN111315759B (zh) 2023-10-13
EP3708575A4 (en) 2021-10-27

Similar Documents

Publication Publication Date Title
WO2019074333A3 (ko) 티오레독신 1 에피토프 및 이에 특이적으로 결합하는 단일클론항체
JP2017518366A5 (ko)
Lee et al. Diagnostic significance of serum HMGB1 in colorectal carcinomas
MX2019008221A (es) Anticuerpo anti-gpc3.
MA46299B1 (fr) Molécule de polypeptide à double spécificité améliorée
JP2018042573A5 (ko)
BRPI0607450A2 (pt) anticorpos que se ligam a epha2 e métodos para sua utilização
JP2015533788A5 (ko)
EA202091810A1 (ru) Антитела к b7-h4 и способы их применения
ATE509033T1 (de) Manipulierte anti-prostatastammzellenantigen (psca)-antikörper für krebs-targeting
WO2009019365A3 (fr) Procede de dosage de l'ezrine pour le diagnostic in vitro du cancer colorectal
JP2016516008A5 (ko)
MX2016004239A (es) Anticuerpos anti-epcam y métodos de uso.
GB0616929D0 (en) Antibodies, assays and hybridomas
MX2020010094A (es) Kir3dl3 como receptor de hhla2, anticuerpos anti-hhla2 y usos de estos.
JP2020510409A5 (ko)
Levin et al. Development of a reliable assay to measure glypican-1 in plasma and serum reveals circulating glypican-1 as a novel prostate cancer biomarker
US20110177525A1 (en) Antibodies and methods of diagnosing diseases
Li et al. Sandwich ELISA for detecting urinary Survivin in bladder cancer
Qin et al. Autoantibody against 14-3-3 zeta: a serological marker in detection of gastric cancer
JP2018520366A5 (ko)
MX2022002672A (es) Anticuerpo anti-vsig4 o fragmento de union a antigeno y usos del mismo.
MY194325A (en) Test object detection method, and immunoassay instrument and monoclonal antibody for same
BR112022004570A2 (pt) Terapia de combinação com anticorpos entpd2 e cd73
Li et al. Preparation and characterization of anti-GP73 monoclonal antibodies and development of double-antibody sandwich ELISA

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18866509

Country of ref document: EP

Kind code of ref document: A2

ENP Entry into the national phase

Ref document number: 2020520447

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2018349387

Country of ref document: AU

Date of ref document: 20181012

Kind code of ref document: A

ENP Entry into the national phase

Ref document number: 2018866509

Country of ref document: EP

Effective date: 20200512

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112020007326

Country of ref document: BR

ENP Entry into the national phase

Ref document number: 112020007326

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20200413