WO2018030569A1 - Composition cosmétique de type maquillage contenant des protéines d'adhérence issues de la moule - Google Patents

Composition cosmétique de type maquillage contenant des protéines d'adhérence issues de la moule Download PDF

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Publication number
WO2018030569A1
WO2018030569A1 PCT/KR2016/009266 KR2016009266W WO2018030569A1 WO 2018030569 A1 WO2018030569 A1 WO 2018030569A1 KR 2016009266 W KR2016009266 W KR 2016009266W WO 2018030569 A1 WO2018030569 A1 WO 2018030569A1
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WO
WIPO (PCT)
Prior art keywords
mussel adhesive
cosmetic composition
protein
adhesive protein
makeup cosmetic
Prior art date
Application number
PCT/KR2016/009266
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English (en)
Korean (ko)
Inventor
이윤진
신대현
차윤정
박성길
이선혜
Original Assignee
소망화장품주식회사
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Publication of WO2018030569A1 publication Critical patent/WO2018030569A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
    • A61Q1/02—Preparations containing skin colorants, e.g. pigments
    • A61Q1/04—Preparations containing skin colorants, e.g. pigments for lips
    • A61Q1/06—Lipsticks
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
    • A61Q1/02—Preparations containing skin colorants, e.g. pigments
    • A61Q1/10—Preparations containing skin colorants, e.g. pigments for eyes, e.g. eyeliner, mascara

Definitions

  • the present invention relates to a make-up cosmetic composition containing the mussel adhesive protein, and more particularly to a technology for producing a make-up cosmetic composition excellent in adhesion, persistence, dispersibility and usability by containing a human-friendly hybrid mussel adhesive protein. .
  • Makeup cosmetics are generally used to conceal skin defects and to control the beautiful skin color to make the skin young and healthy. For this purpose, make-up cosmetics should be applied thinly to the skin, eyebrows, lips, etc., without exerting a burden on the skin and exhibiting a high skin color correction effect.
  • Inorganic powders are used in the makeup cosmetics, and typical examples thereof include talc, mica, sericite, kaolin, titanium dioxide, zinc oxide, and iron oxide.
  • these inorganic powders themselves have a poor adhesion to the skin and tend to fall off after a long time of application or to fall off by water or sweat, or the powders aggregate and aggregate together. This situation is often inconvenient to repair the makeup, and the powder may clog the pores, and in severe cases may provide a cause of skin diseases.
  • Inorganic powder used in make-up cosmetics should be easily applied to skin, eyebrows, etc., and when applied, should give a smooth feeling, have water / oil resistance that is not affected by water or oil, and keep the applied state. It should be provided with adhesion and skin affinity. For this purpose, it may be considered to formulate by mixing a specific supplement material in the powder, but there is a problem in that the formulation is limited due to the characteristics that the solid powder is used as a raw material of the makeup cosmetics.
  • Makeup cosmetic composition with excellent gloss and usability includes "dipropylene glycol dibenzoate, C12 to C15 alkyl benzoate, phenyldimethicone, diphenylsiloxyphenyltrimeth on the surface of the pigment powder.
  • the present invention discloses a makeup cosmetic composition comprising a composite powder having a coating layer comprising one kind of oil selected from the group consisting of chicon and combinations thereof, and Korean Patent Publication No.
  • Cosmetic powder coated with cosmetics and make-up cosmetic composition containing the same is coated with at least one of acrylate silicone polymer used as a contact lens material and a polyurethane having a similar transparency and refractive index to a cosmetic surface having a smooth surface.
  • Cosmetic nose manufactured to allow natural and transparent makeup The powders and make-up cosmetic composition containing the same, are disclosed.
  • the inventors of the present invention are developing functional cosmetics utilizing mussel adhesive proteins, which are human and environmentally friendly, and formulating hybrid cosmetic mussel adhesive proteins prepared by combining mussel adhesive proteins in a proper order and molecular weight, such as foundation, mascara, and lipstick.
  • the present invention was completed by confirming that a makeup cosmetic composition having excellent adhesion, durability, dispersibility, and usability when applied to manufacture can be prepared.
  • an object of the present invention is to provide a makeup cosmetic composition containing a hybrid mussel adhesive protein with excellent adhesion, durability, dispersibility and usability.
  • a makeup cosmetic composition containing a hybrid mussel adhesive protein.
  • hybrid mussel adhesive protein consists of the amino acid sequence of SEQ ID NO: 1.
  • the hybrid mussel adhesive protein is contained 0.5 to 5% by weight based on the total weight of the cosmetic composition.
  • the makeup cosmetic composition may be a makeup base, foundation, BB cream, concealer, lipstick, lip gloss, lip liner, powder, two-way cake, primer, fact, powder, eye shadow, blusher, eye liner, eye mascara, hair gel, hair mascara or It may have a formulation of nail enamel.
  • the makeup cosmetic composition of the present invention is excellent in adhesion, durability, dispersibility, biocompatibility and usability because it contains a mussel adhesive protein which is human-friendly and exhibits excellent adhesion even in a wet environment.
  • 1 is a schematic diagram showing the structure of the present invention hybrid mussel adhesive protein.
  • FIG. 2 is a graph showing the stability of the W / O foundation prepared according to an embodiment of the present invention.
  • FIG 3 is a graph showing the stability of the O / W foundation prepared according to an embodiment of the present invention.
  • Figure 4 is a photograph showing the volume, application and agglomeration of mascara prepared according to an embodiment of the present invention.
  • Marine life mussels produce and secrete adhesive proteins that allow mussels to attach themselves to wet solid surfaces, such as rocks in the sea, which can resist strong waves and buoyancy in the sea. have.
  • Mussel adhesive proteins produced and secreted from mussels are classified using the word fp, which stands for foot protein, to distinguish between types and properties. This is because the mussel adhesive protein is secreted only from the mussel's feet.
  • fp-1 to fp-6 A total of six mussel adhesive proteins have been discovered to date, and each mussel adhesive protein is named fp-1 to fp-6. Of these, fp-1, fp-3, and fp-5 proteins are believed to be directly involved in adhesion and coating.
  • DOPA 3,4-dihydroxyphenylalanine
  • DOPA is a protein component of amino acids tyrosine is hydrated by a polyphenol oxidant to form a very strong hydrogen bond with the hydrophilic surface, because it can also bind to hydrophobic or metal surfaces containing aromatics Mussels can adhere to a wide variety of surfaces, including glass, metal, plastic, and teflon. DOPA also covalently binds to other amino acids, such as lysine or another DOPA, present in a high proportion of mussel adhesive proteins, which quickly bonds to the surface of proteins, resulting in stronger adhesion than chemical adhesives such as epoxy and phenolic resins. Can be.
  • mussel bioadhesives are more resistant to water as they get wet, so they can be applied as technological materials for underwater construction. When used in a living body, it does not attack human cells or cause an immune response, so it can be used for adhesion of living tissue without having to sew a thread during surgery, and a large application for medical use is possible.
  • the present inventors hybridized each mussel adhesive protein, to prepare a hybrid mussel adhesive protein having a different composition and molecular weight, and studied to apply it to a makeup cosmetic composition.
  • Hybrid mussel adhesive proteins having various adhesive strengths were designed to find the most suitable fusion adhesive proteins for use in the preparation of makeup cosmetic compositions.
  • the present invention relates to a makeup cosmetic composition containing a hybrid mussel adhesive protein.
  • a makeup cosmetic composition containing a hybrid mussel adhesive protein consisting of the amino acid sequence of SEQ ID NO: 1.
  • Hybrid mussel adhesive protein consisting of the amino acid sequence of SEQ ID NO: 1 is the first one fp-1 protein (fp-1), the third one protein (fp-3), the first one protein (fp-1), 5 It is a fusion of the pomaceous protein (fp-5) and No. 1 liposomal protein (fp-1), and has a molecular weight of 37 kda (FIG. 1).
  • the hybrid mussel adhesive protein can be inserted into a conventional vector designed to express an external gene so that the hybrid mussel adhesive protein can be mass-produced by a genetic engineering method.
  • the vector may be appropriately selected or newly produced according to the type and characteristics of the host cell for producing a protein.
  • An example of producing the hybrid mussel adhesive protein by genetic engineering method is as follows. First, a vector comprising a gene encoding the hybrid mussel adhesive protein is expressed. The vector is transformed into a host cell to prepare a transformant. The transformant is cultured to produce a hybrid mussel adhesive protein. The method of transforming the vector into a host cell and producing a recombinant protein from the transformant can be easily carried out by conventional methods.
  • the recombinant protein expressed by the above method is isolated and purified according to the expression pattern.
  • the supernatant of the cell lysate can be chromatographed with a column filled with an affinity resin such as nickel resin to purify the recombinant protein.
  • the cell by-products (pellets) of the cell debris are suspended in an acidic organic solvent, preferably an organic solvent of pH 3-6, to prepare a suspension, and then the suspension is centrifuged to separate the supernatant.
  • Recombinant protein can be purified.
  • Acetic acid, citric acid and lactic acid may be used as the acidic organic solvent, but is not limited thereto.
  • acetic acid cell byproducts (pellets) are dissolved in 5-30 (v / v)% acetic acid solution.
  • the supernatant obtained by acidic organic solvent treatment can also be subjected to gel filtration chromatography to further purify the recombinant protein.
  • the hybrid mussel adhesive protein thus prepared showed excellent adhesion, biocompatibility and phase stability in various formulations of makeup cosmetics.
  • the hybrid mussel adhesive protein is contained 0.5 to 5% by weight based on the total weight of the cosmetic composition. If the content is less than 0.5% by weight, the adhesion is weak, and if it exceeds 5% by weight, there may be agglomeration.
  • the makeup cosmetic composition may be a makeup base, foundation, BB cream, concealer, lipstick, lip gloss, lip liner, powder, two-way cake, primer, fact, powder, eye shadow, blusher, eye liner, eye mascara, hair gel, hair mascara or It can have various formulations of nail enamels.
  • An E. coli BL21 (DE3) transformant comprising an expression gene of a hybrid mussel adhesive protein having the amino acid sequence of SEQ ID NO: 1 was prepared.
  • E. coli BL21 (DE3) was incubated in LB (5 g / L yeast extract, 10 g / L Tryptone and 10 g / L NaCl) medium, and the final concentration of IPTG was reached when the absorbance of the medium reached 0.6 at 600 nm. Addition at 1 mM induced expression of the hybrid mussel adhesive protein. E. coli BL21 (DE3) culture was centrifuged at 13,000 rpm for 4-10 minutes to obtain cell pellets, which were stored at -80 ° C.
  • the obtained cell pellet was stirred using lysis buffer (2.4 g / L sodium phosphate monobasic, 5.6 g / L sodium phosphate dibasic, 10 mM EDTA and 1% Triton X-100) and the cells were disrupted using a high pressure crusher. .
  • the lysate was centrifuged at 9,000 rpm for 20 minutes to obtain insoluble protein aggregates containing mussel adhesive protein.
  • Hybrid mussel adhesive protein was extracted from insoluble protein aggregates using 25% acetic acid and centrifuged at 9,000 rpm for 20 minutes to recover supernatant containing mussel adhesive protein.
  • the recovered supernatant was raised to pH 12.8 using 10N NaOH.
  • the supernatant was recovered by centrifugation under the same conditions.
  • the supernatant was neutralized and titrated to pH 6-7 using acetic acid and then centrifuged under the same conditions to obtain a precipitate of the hybrid mussel adhesive protein.
  • the obtained precipitate was dissolved in an appropriate amount of purified water and then lyophilized to obtain a lyophilized hybrid mussel adhesive protein having a purity of 90% or more.
  • Test Example One hybrid mussel Adhesive protein Skin stability check
  • the 0.1-10.0% mussel adhesive protein solution showed no erythema, and the 20.0% mussel adhesive protein solution showed mild stimulation of weak erythema or some red spots with an average of 1.05.
  • Example 2 ⁇ 6 hybrid mussel Adhesion Protein Preparation of Containing W / O Foundation
  • Test Example 2 hybrid mussel Adhesion Protein Adhesion and Stability Test of Containing W / O Foundation
  • Comparative Example 1 Examples 2-6 density 0% 0.5% 1.0% 2.0% 3.0% 4.0% 25 °C ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇
  • Target type Compression, Target Value: 10.0mm, Trigger Load: 1.0g, Test Speed: 1.0mm / s, Sample Temperature: 25 ⁇ 3 °C
  • Example 2 Comparative Example 1 0.12 ⁇ 0.01
  • Example 6 (MP% 4.0%) 0.94 ⁇ 0.01
  • Example 7-11 hybrid mussel Adhesion Protein Preparation of Containing O / W Foundation
  • Test Example 3 hybrid mussel Adhesion Protein Adhesion and Safety Test of Containing O / W Foundation
  • Comparative Example 2 Examples 7-11 density 0% 0.5% 1.0% 2.0% 3.0% 4.0% 25 °C ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇
  • Target type Compression, Target Value: 10.0mm, Trigger Load: 1.0g, Test Speed: 1.0mm / s, Sample Temperature: 25 ⁇ 3 °C
  • Example 7 (MP 0.5%) 0.19 ⁇ 0.01
  • Example 9 (MP% 2.0%) 0.37 ⁇ 0.01
  • Example 11 (MP% 4.0%) 0.54 ⁇ 0.01
  • Example 12-14 hybrid mussel Adhesion Protein Preparation of Containing Mascara
  • Mascara was prepared by adding mussel adhesive protein by concentration by adjusting the amount of aqueous phase.
  • Test Example 4 hybrid mussel Adhesion Protein Evaluation of containing mascara
  • each adhesion was measured using a texture analyzer (CT3, Brookfield Engineering Laboratories, Inc, U.S.A). The measurement was performed by reading the maximum force (g) and area (mJ) required when exiting at a speed of 1.0mm / s by 10mm depth using a TA-23 probe with a model and a touch similar to a human finger.
  • CT3 Brookfield Engineering Laboratories, Inc, U.S.A
  • Target type Compression, Target Value: 10.0mm, Trigger Load: 1.0g, Test Speed: 1.0mm / s, Sample Temperature: 25 ⁇ 3 °C
  • Example 12 Comparative Example 3 0.12 ⁇ 0.01
  • Example 12 (MP% 0.2%) 0.31 ⁇ 0.02
  • Example 13 (MP% 2.0%) 0.72 ⁇ 0.02
  • Example 14 (MP% 5.0%) 0.95 ⁇ 0.01
  • Example 12 Example 13

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Abstract

La présente invention concerne une composition cosmétique de type maquillage contenant des protéines d'adhérence issues de la moule et, plus précisément, une composition cosmétique de type maquillage contenant des protéines d'adhérence issues de la moule, qui sont respectueuses de l'homme et manifestent une excellente force d'adhérence même dans un environnement humide, ce qui leur confère ainsi une excellente propriété d'adhérence, durabilité, dispersibilité, bioaffinité et texture.
PCT/KR2016/009266 2016-08-12 2016-08-22 Composition cosmétique de type maquillage contenant des protéines d'adhérence issues de la moule WO2018030569A1 (fr)

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KR20160102975 2016-08-12

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116617109A (zh) * 2023-06-05 2023-08-22 广州植境生物科技有限公司 一种贻贝粘蛋白头皮修护组合物及其制备方法和应用
CN118121504A (zh) * 2024-05-06 2024-06-04 深圳柏垠生物科技有限公司 一种基于贻贝粘蛋白的组合物及制备方法与应用
KR102706467B1 (ko) * 2023-10-17 2024-09-13 주식회사 씨앤드케이 저자극성 피부 세정용 친환경 화장료 조성물 및 이의 제조 방법

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WO2011115420A2 (fr) * 2010-03-16 2011-09-22 Kollodis Biosciences, Inc. Mimétique de matrice extracellulaire adhésive
KR20120013626A (ko) * 2010-08-05 2012-02-15 포항공과대학교 산학협력단 홍합 접착 단백질 또는 이의 변이체에 음이온성 고분자로 형성된 코아세르베이트
KR101392983B1 (ko) * 2012-11-16 2014-05-08 포항공과대학교 산학협력단 홍합 접착 단백질을 이용한 인공모발 접착제 및 이의 제조 방법
CN104323927A (zh) * 2014-11-06 2015-02-04 济南星河康泰贸易有限公司 贻贝粘蛋白在制备皮肤美容化妆品上的应用

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WO2011115420A2 (fr) * 2010-03-16 2011-09-22 Kollodis Biosciences, Inc. Mimétique de matrice extracellulaire adhésive
KR20120013626A (ko) * 2010-08-05 2012-02-15 포항공과대학교 산학협력단 홍합 접착 단백질 또는 이의 변이체에 음이온성 고분자로 형성된 코아세르베이트
KR101392983B1 (ko) * 2012-11-16 2014-05-08 포항공과대학교 산학협력단 홍합 접착 단백질을 이용한 인공모발 접착제 및 이의 제조 방법
CN104323927A (zh) * 2014-11-06 2015-02-04 济南星河康泰贸易有限公司 贻贝粘蛋白在制备皮肤美容化妆品上的应用

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116617109A (zh) * 2023-06-05 2023-08-22 广州植境生物科技有限公司 一种贻贝粘蛋白头皮修护组合物及其制备方法和应用
CN116617109B (zh) * 2023-06-05 2024-02-20 广州植境生物科技有限公司 一种贻贝粘蛋白头皮修护组合物及其制备方法和应用
KR102706467B1 (ko) * 2023-10-17 2024-09-13 주식회사 씨앤드케이 저자극성 피부 세정용 친환경 화장료 조성물 및 이의 제조 방법
CN118121504A (zh) * 2024-05-06 2024-06-04 深圳柏垠生物科技有限公司 一种基于贻贝粘蛋白的组合物及制备方法与应用

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