WO2018030569A1 - Makeup cosmetic composition containing mussel adhesive proteins - Google Patents

Abstract

The present invention relates to a makeup cosmetic composition containing mussel adhesive proteins and, specifically, to a makeup cosmetic composition containing mussel adhesive proteins, which are human-friendly and exhibit excellent adhesive strength even in a humid environment, thereby having an excellent adhesive property, sustainability, dispersibility, bioaffinity, and texture.

Description

Makeup cosmetic composition containing mussel adhesive protein

The present invention relates to a make-up cosmetic composition containing the mussel adhesive protein, and more particularly to a technology for producing a make-up cosmetic composition excellent in adhesion, persistence, dispersibility and usability by containing a human-friendly hybrid mussel adhesive protein. .

Makeup cosmetics are generally used to conceal skin defects and to control the beautiful skin color to make the skin young and healthy. For this purpose, make-up cosmetics should be applied thinly to the skin, eyebrows, lips, etc., without exerting a burden on the skin and exhibiting a high skin color correction effect. Inorganic powders are used in the makeup cosmetics, and typical examples thereof include talc, mica, sericite, kaolin, titanium dioxide, zinc oxide, and iron oxide. However, these inorganic powders themselves have a poor adhesion to the skin and tend to fall off after a long time of application or to fall off by water or sweat, or the powders aggregate and aggregate together. This situation is often inconvenient to repair the makeup, and the powder may clog the pores, and in severe cases may provide a cause of skin diseases.

Inorganic powder used in make-up cosmetics should be easily applied to skin, eyebrows, etc., and when applied, should give a smooth feeling, have water / oil resistance that is not affected by water or oil, and keep the applied state. It should be provided with adhesion and skin affinity. For this purpose, it may be considered to formulate by mixing a specific supplement material in the powder, but there is a problem in that the formulation is limited due to the characteristics that the solid powder is used as a raw material of the makeup cosmetics.

In order to solve this problem, a method of appropriately coating the powder surface has been attempted. As a representative coating material, water-soluble moisturizers for improving moisturizing properties, oily ingredients for improving water resistance, fluorine compounds, silicone polymer components for improving water resistance and usability have been proposed.

Republic of Korea Patent Publication No. 2014-0072548 "Makeup cosmetic composition with excellent gloss and usability" includes "dipropylene glycol dibenzoate, C12 to C15 alkyl benzoate, phenyldimethicone, diphenylsiloxyphenyltrimeth on the surface of the pigment powder. The present invention discloses a makeup cosmetic composition comprising a composite powder having a coating layer comprising one kind of oil selected from the group consisting of chicon and combinations thereof, and Korean Patent Publication No. 2012-0026234 Cosmetic powder coated with cosmetics and make-up cosmetic composition containing the same "is coated with at least one of acrylate silicone polymer used as a contact lens material and a polyurethane having a similar transparency and refractive index to a cosmetic surface having a smooth surface. Cosmetic nose manufactured to allow natural and transparent makeup The powders and make-up cosmetic composition containing the same, are disclosed.

The inventors of the present invention are developing functional cosmetics utilizing mussel adhesive proteins, which are human and environmentally friendly, and formulating hybrid cosmetic mussel adhesive proteins prepared by combining mussel adhesive proteins in a proper order and molecular weight, such as foundation, mascara, and lipstick. The present invention was completed by confirming that a makeup cosmetic composition having excellent adhesion, durability, dispersibility, and usability when applied to manufacture can be prepared.

Therefore, an object of the present invention is to provide a makeup cosmetic composition containing a hybrid mussel adhesive protein with excellent adhesion, durability, dispersibility and usability.

According to the present invention to achieve the above object there is provided a makeup cosmetic composition containing a hybrid mussel adhesive protein.

Preferably the hybrid mussel adhesive protein consists of the amino acid sequence of SEQ ID NO: 1.

The hybrid mussel adhesive protein is contained 0.5 to 5% by weight based on the total weight of the cosmetic composition.

The makeup cosmetic composition may be a makeup base, foundation, BB cream, concealer, lipstick, lip gloss, lip liner, powder, two-way cake, primer, fact, powder, eye shadow, blusher, eye liner, eye mascara, hair gel, hair mascara or It may have a formulation of nail enamel.

The makeup cosmetic composition of the present invention is excellent in adhesion, durability, dispersibility, biocompatibility and usability because it contains a mussel adhesive protein which is human-friendly and exhibits excellent adhesion even in a wet environment.

1 is a schematic diagram showing the structure of the present invention hybrid mussel adhesive protein.

2 is a graph showing the stability of the W / O foundation prepared according to an embodiment of the present invention.

3 is a graph showing the stability of the O / W foundation prepared according to an embodiment of the present invention.

Figure 4 is a photograph showing the volume, application and agglomeration of mascara prepared according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail.

Marine life mussels produce and secrete adhesive proteins that allow mussels to attach themselves to wet solid surfaces, such as rocks in the sea, which can resist strong waves and buoyancy in the sea. have. Mussel adhesive proteins produced and secreted from mussels are classified using the word fp, which stands for foot protein, to distinguish between types and properties. This is because the mussel adhesive protein is secreted only from the mussel's feet. A total of six mussel adhesive proteins have been discovered to date, and each mussel adhesive protein is named fp-1 to fp-6. Of these, fp-1, fp-3, and fp-5 proteins are believed to be directly involved in adhesion and coating.

In all mussel adhesive proteins, a substance called 3,4-dihydroxyphenylalanine (DOPA) is commonly found. In water, strong adhesion on various surfaces is derived from this high concentration of DOPA in the adhesive protein. It is becoming.

DOPA is a protein component of amino acids tyrosine is hydrated by a polyphenol oxidant to form a very strong hydrogen bond with the hydrophilic surface, because it can also bind to hydrophobic or metal surfaces containing aromatics Mussels can adhere to a wide variety of surfaces, including glass, metal, plastic, and teflon. DOPA also covalently binds to other amino acids, such as lysine or another DOPA, present in a high proportion of mussel adhesive proteins, which quickly bonds to the surface of proteins, resulting in stronger adhesion than chemical adhesives such as epoxy and phenolic resins. Can be.

In addition, unlike conventional chemical adhesives, which have poor adhesion in humid places or water, mussel bioadhesives are more resistant to water as they get wet, so they can be applied as groundbreaking materials for underwater construction. When used in a living body, it does not attack human cells or cause an immune response, so it can be used for adhesion of living tissue without having to sew a thread during surgery, and a large application for medical use is possible.

The present inventors hybridized each mussel adhesive protein, to prepare a hybrid mussel adhesive protein having a different composition and molecular weight, and studied to apply it to a makeup cosmetic composition. Hybrid mussel adhesive proteins having various adhesive strengths were designed to find the most suitable fusion adhesive proteins for use in the preparation of makeup cosmetic compositions.

The present invention relates to a makeup cosmetic composition containing a hybrid mussel adhesive protein. Preferably it relates to a makeup cosmetic composition containing a hybrid mussel adhesive protein consisting of the amino acid sequence of SEQ ID NO: 1.

Hybrid mussel adhesive protein (fp-13151) consisting of the amino acid sequence of SEQ ID NO: 1 is the first one fp-1 protein (fp-1), the third one protein (fp-3), the first one protein (fp-1), 5 It is a fusion of the pomaceous protein (fp-5) and No. 1 liposomal protein (fp-1), and has a molecular weight of 37 kda (FIG. 1).

The hybrid mussel adhesive protein can be inserted into a conventional vector designed to express an external gene so that the hybrid mussel adhesive protein can be mass-produced by a genetic engineering method. The vector may be appropriately selected or newly produced according to the type and characteristics of the host cell for producing a protein.

An example of producing the hybrid mussel adhesive protein by genetic engineering method is as follows. First, a vector comprising a gene encoding the hybrid mussel adhesive protein is expressed. The vector is transformed into a host cell to prepare a transformant. The transformant is cultured to produce a hybrid mussel adhesive protein. The method of transforming the vector into a host cell and producing a recombinant protein from the transformant can be easily carried out by conventional methods.

The recombinant protein expressed by the above method is isolated and purified according to the expression pattern. When expressed in water-soluble form, the supernatant of the cell lysate can be chromatographed with a column filled with an affinity resin such as nickel resin to purify the recombinant protein. When expressed in a non-aqueous form, the cell by-products (pellets) of the cell debris are suspended in an acidic organic solvent, preferably an organic solvent of pH 3-6, to prepare a suspension, and then the suspension is centrifuged to separate the supernatant. Recombinant protein can be purified. Acetic acid, citric acid and lactic acid may be used as the acidic organic solvent, but is not limited thereto. In the case of acetic acid, cell byproducts (pellets) are dissolved in 5-30 (v / v)% acetic acid solution. The supernatant obtained by acidic organic solvent treatment can also be subjected to gel filtration chromatography to further purify the recombinant protein.

The hybrid mussel adhesive protein thus prepared showed excellent adhesion, biocompatibility and phase stability in various formulations of makeup cosmetics.

The hybrid mussel adhesive protein is contained 0.5 to 5% by weight based on the total weight of the cosmetic composition. If the content is less than 0.5% by weight, the adhesion is weak, and if it exceeds 5% by weight, there may be agglomeration.

The makeup cosmetic composition may be a makeup base, foundation, BB cream, concealer, lipstick, lip gloss, lip liner, powder, two-way cake, primer, fact, powder, eye shadow, blusher, eye liner, eye mascara, hair gel, hair mascara or It can have various formulations of nail enamels.

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples.

Example  One: hybrid  mussel Adhesive protein  Produce

An E. coli BL21 (DE3) transformant comprising an expression gene of a hybrid mussel adhesive protein having the amino acid sequence of SEQ ID NO: 1 was prepared.

E. coli BL21 (DE3) was incubated in LB (5 g / L yeast extract, 10 g / L Tryptone and 10 g / L NaCl) medium, and the final concentration of IPTG was reached when the absorbance of the medium reached 0.6 at 600 nm. Addition at 1 mM induced expression of the hybrid mussel adhesive protein. E. coli BL21 (DE3) culture was centrifuged at 13,000 rpm for 4-10 minutes to obtain cell pellets, which were stored at -80 ° C.

The obtained cell pellet was stirred using lysis buffer (2.4 g / L sodium phosphate monobasic, 5.6 g / L sodium phosphate dibasic, 10 mM EDTA and 1% Triton X-100) and the cells were disrupted using a high pressure crusher. . The lysate was centrifuged at 9,000 rpm for 20 minutes to obtain insoluble protein aggregates containing mussel adhesive protein. Hybrid mussel adhesive protein was extracted from insoluble protein aggregates using 25% acetic acid and centrifuged at 9,000 rpm for 20 minutes to recover supernatant containing mussel adhesive protein. The recovered supernatant was raised to pH 12.8 using 10N NaOH. The supernatant was recovered by centrifugation under the same conditions. The supernatant was neutralized and titrated to pH 6-7 using acetic acid and then centrifuged under the same conditions to obtain a precipitate of the hybrid mussel adhesive protein. The obtained precipitate was dissolved in an appropriate amount of purified water and then lyophilized to obtain a lyophilized hybrid mussel adhesive protein having a purity of 90% or more.

Test Example  One: hybrid  mussel Adhesive protein  Skin stability check

Skin safety was evaluated in 20 subjects (mean age 32.5 ± 7) (patch test). 0.1 ml of mussel adhesive protein solution dissolved in distilled water by concentration was added to the Finn chamber and attached to the inner side of the arm of 20 adult men and women and removed after about 24 hours. After removing the patch, the erythema that appeared after 30 minutes of washing the skin under running water was judged by evaluation criteria and an average irritation value was obtained. The results are shown in Table 1 below.

density	0.1%	0.5%	1.0%	5.0%	10.0%	20.0%
Mean stimulus value	0	0	0	0	0	1.05

Evaluation standard

(0: no erythema 1: weak erythema, red spot form 2: irregular erythema state 3: weak erythema 4: strong erythema 5: reddish brown blister, etc.)

As confirmed in Table 1, the 0.1-10.0% mussel adhesive protein solution showed no erythema, and the 20.0% mussel adhesive protein solution showed mild stimulation of weak erythema or some red spots with an average of 1.05.

Example  2 ~ 6: hybrid  mussel Adhesion Protein  Preparation of Containing W / O Foundation

To prepare a W / O type foundation in a conventional manner according to the composition of Table 2. The amount of aqueous phase was adjusted to prepare a W / O foundation in which mussel adhesive protein was added for each concentration.

Component	Content (Wt%)
	Comparative Example 1	Example
		2	3	4	5	6
D.I-Water	To 100	To 100	To 100	To 100	To 100	To 100
Disodium EDTA	0.05	0.05	0.05	0.05	0.05	0.05
Glycerin	5.0	5.0	5.0	5.0	5.0	5.0
Butylene glycol	5.0	5.0	5.0	5.0	5.0	5.0
Sodium Chloride	1.0	1.0	1.0	1.0	1.0	1.0
Cyclopentasiloxane	6.0	6.0	6.0	6.0	6.0	6.0
Bentone Gel	4.0	4.0	4.0	4.0	4.0	4.0
PEG-10 Dimethicone	2.0	2.0	2.0	2.0	2.0	2.0
Sorbitan Isostearate	0.1	0.1	0.1	0.1	0.1	0.1
Cetyl Ethylhexanoate	1.0	1.0	1.0	1.0	1.0	1.0
KSG-16	6.0	6.0	6.0	6.0	6.0	6.0
Pentaerythrityl Tetraethylhexanoate	0.3	0.3	0.3	0.3	0.3	0.3
Dicaprylyl Carbonate	0.3	0.3	0.3	0.3	0.3	0.3
Titanium Dioxide	8.0	8.0	8.0	8.0	8.0	8.0
CI 77492	1.0	1.0	1.0	1.0	1.0	1.0
CI 77491	0.2	0.2	0.2	0.2	0.2	0.2
CI 77499	0.05	0.05	0.05	0.05	0.05	0.05
Silica	0.3	0.3	0.3	0.3	0.3	0.3
Ethyl Alcohol	2.0	2.0	2.0	2.0	2.0	2.0
Preservative	q.s.	q.s.	q.s.	q.s.	q.s.	q.s.
Mussel adhesive proteins (Example 1)	-	0.5	1.0	2.0	3.0	4.0

Test Example  2: hybrid  mussel Adhesion Protein  Adhesion and Stability Test of Containing W / O Foundation

Stability test

W / O foundation to which the mussel adhesive protein (MP) is added for each concentration prepared in Example In order to evaluate the stability, visual observation (phase separation), pH, and hardness (hardness) were measured for 8 weeks at 25 ° C at 1 week intervals.

The sample was observed at 25 ° C. for 8 weeks and no phase separation was observed (Table 3). Also, as shown in FIG. 2, all of them were stable without significant change in hardness and pH.

	Comparative Example 1	Examples 2-6
density	0%	0.5%	1.0%	2.0%	3.0%	4.0%
25 ℃	◎	◎	◎	◎	◎	◎

(◎: stable, △: abnormality sign, X: unstable)

Adhesion  exam

Texture analyzer (CT3, Brookfield Engineering Laboratories, Inc., USA) for the foundation of Comparative Examples and Examples to determine whether the adhesion of mussel adhesive protein (MP) affects the skin adhesion (adhesion) of the W / O foundation. Each adhesion was measured using. The measurement was performed by reading the maximum force (g) and area (mJ) required when exiting at a speed of 1.0mm / s by 10mm depth using a TA-23 probe with a model and a touch similar to a human finger.

[Experimental Conditions]

Target type: Compression, Target Value: 10.0mm, Trigger Load: 1.0g, Test Speed: 1.0mm / s, Sample Temperature: 25 ± 3 ℃

The results are shown in Table 4 below.

Sample	Adhesiveness (mJ)
Comparative Example 1	0.12 ± 0.01
Example 2 (MP 0.5%)	0.71 ± 0.01
Example 3 (MP = 1.0%)	0.73 ± 0.02
Example 4 (MP = 2.0%)	0.82 ± 0.02
Example 5 (MP = 3.0%)	0.88 ± 0.02
Example 6 (MP% 4.0%)	0.94 ± 0.01

As confirmed in Table 4, the adhesion of the W / O foundation of the example containing the hybrid mussel adhesive protein by concentration was measured very high compared to the formulation of Comparative Example 1 does not contain it.

Example  7-11: hybrid  mussel Adhesion Protein  Preparation of Containing O / W Foundation

To prepare a O / W type foundation in a conventional manner according to the composition of Table 5. The amount of aqueous phase was adjusted to prepare an O / W foundation in which mussel adhesive protein was added for each concentration.

Component	Content (Wt%)
	Comparative Example 2	Example
		7	8	9	10	11
D.I-Water	To 100	To 100	To 100	To 100	To 100	To 100
Disodium EDTA	0.05	0.05	0.05	0.05	0.05	0.05
Glycerin	5.0	5.0	5.0	5.0	5.0	5.0
Butylene glycol	5.0	5.0	5.0	5.0	5.0	5.0
Acrylates / C10-30 Alkyl Acrylate Crosspolymer	0.2	0.2	0.2	0.2	0.2	0.2
Xanthan Gum	0.01	0.01	0.01	0.01	0.01	0.01
Polysorbate 60	1.0	1.0	1.0	1.0	1.0	1.0
Sorbitan Stearate	0.5	0.5	0.5	0.5	0.5	0.5
Stearic acid	1.0	1.0	1.0	1.0	1.0	1.0
Cetearyl alcohol	1.0	1.0	1.0	1.0	1.0	1.0
Glyceryl Stearate	0.5	0.5	0.5	0.5	0.5	0.5
Montanov 202	0.5	0.5	0.5	0.5	0.5	0.5
Caprylyl methicone	5.0	5.0	5.0	5.0	5.0	5.0
Titanium Dioxide	8.0	8.0	8.0	8.0	8.0	8.0
Aluminum Starch Octenylsuccinate	8.0	8.0	8.0	8.0	8.0	8.0
Cyclopentasiloxane	1.0	1.0	1.0	1.0	1.0	1.0
Sorbitan Sesquioleate	0.1	0.1	0.1	0.1	0.1	0.1
CI 77492	1.0	1.0	1.0	1.0	1.0	1.0
CI 77491	0.2	0.2	0.2	0.2	0.2	0.2
CI 77499	0.05	0.05	0.05	0.05	0.05	0.05
Preservative	q.s.	q.s.	q.s.	q.s.	q.s.	q.s.
Mussel adhesive proteins (Example 1)	-	0.5	1.0	2.0	3.0	4.0

Test Example  3: hybrid  mussel Adhesion Protein  Adhesion and Safety Test of Containing O / W Foundation

Stability test

O / W foundation to which the mussel adhesive protein (MP) is added for each concentration of the In order to evaluate stability, visual observation (phase separation), pH, and hardness were measured for 8 weeks at 25 ° C. at 1 week intervals.

The sample was observed at 25 ° C. for 8 weeks and no phase separation was observed (Table 6). In addition, as shown in Figure 3 it was shown that all of the stable without significant changes in hardness and pH.

	Comparative Example 2	Examples 7-11
density	0%	0.5%	1.0%	2.0%	3.0%	4.0%
25 ℃	◎	◎	◎	◎	◎	◎

(◎: stable, △: abnormality sign, X: unstable)

Adhesion  exam

Texture analyzer (CT3, Brookfield Engineering Laboratories, Inc, USA) for the foundation of Comparative Examples and Examples to determine whether the adhesion of mussel adhesive protein (MP) affects the skin adhesion (adhesion) of O / W foundation. Each adhesion was measured using. The measurement was performed by reading the maximum force (g) and area (mJ) required when exiting at a speed of 1.0mm / s by 10mm depth using a TA-23 probe with a model and a touch similar to a human finger.

[Experimental Conditions]

Target type: Compression, Target Value: 10.0mm, Trigger Load: 1.0g, Test Speed: 1.0mm / s, Sample Temperature: 25 ± 3 ℃

The results are shown in Table 7 below.

Sample	Adhesiveness (mJ)
Comparative Example 2	0.18 ± 0.01
Example 7 (MP 0.5%)	0.19 ± 0.01
Example 8 (MP = 1.0%)	0.26 ± 0.01
Example 9 (MP% 2.0%)	0.37 ± 0.01
Example 10 (MP = 3.0%)	0.38 ± 0.01
Example 11 (MP% 4.0%)	0.54 ± 0.01

As confirmed in Table 7, the adhesion of the O / W foundation of the Example containing the hybrid mussel adhesive protein by concentration was measured very high compared to the formulation of Comparative Example 2 containing no.

Example  12-14: hybrid  mussel Adhesion Protein  Preparation of Containing Mascara

To prepare a mascara containing a hybrid mussel adhesive protein in a conventional manner according to the composition of Table 8. Mascara was prepared by adding mussel adhesive protein by concentration by adjusting the amount of aqueous phase.

Component	Content (Wt%)
	Comparative Example 3	Example 12	Example 13	Example 14
D.I-Water	To 100	To 100	To 100	To 100
VEEGUM HV	0.25	0.25	0.25	0.25
D.I-Water	9.8	9.8	9.8	9.8
NATROSOL 250HHR	0.2	0.2	0.2	0.2
NIPAGIN M	0.2	0.2	0.2	0.2
NIPA BIOPURE100	0.2	0.2	0.2	0.2
EDTA-2Na	0.05	0.05	0.05	0.05
ALLANTOIN	0.2	0.2	0.2	0.2
D.P.G	0.01	0.01	0.01	0.01
NYLON POLY LW12	0.1	0.1	0.1	0.1
TAROX BL100	7.0	7.0	7.0	7.0
CARNAUBA WAX	7.5	7.5	7.5	7.5
BEES WAX	3.5	3.5	3.5	3.5
STEARIC ACID	8.0	8.0	8.0	8.0
GMS-105	2.0	2.0	2.0	2.0
DC-556	0.2	0.2	0.2	0.2
VITAMIN E ACETATE	0.1	0.1	0.1	0.1
NIPASOL M	0.1	0.1	0.1	0.1
T.E.A	1.8	1.8	1.8	1.8
WATER	5.0	5.0	5.0	5.0
YODOSOL GH 34	40.0	39.8	38.0	35.0
Mussel adhesive Proteins (Example 1)	-	0.2	2	5

Test Example  4: hybrid  mussel Adhesion Protein  Evaluation of containing mascara

Application , Bunching, volumetric evaluation

After the mascara was applied to the hair to evaluate the applicability, agglomeration, and volume of the mascara to which the mussel adhesive protein (MP) was added, the image was confirmed by SEM (Hitachi SU8010). The results are shown in FIG.

As confirmed in FIG. 4, compared to Comparative Example 3, the spreadability and volume of the mascara of the example containing the mussel adhesive protein by concentration were improved. In the case of agglomeration, in the case of Example 14 to which the mussel adhesive protein 5% was applied as compared to other examples, it was confirmed that the agglomeration was slightly aggregated on the hair surface.

Adhesion evaluation

In order to confirm the adhesion of mascara, each adhesion was measured using a texture analyzer (CT3, Brookfield Engineering Laboratories, Inc, U.S.A). The measurement was performed by reading the maximum force (g) and area (mJ) required when exiting at a speed of 1.0mm / s by 10mm depth using a TA-23 probe with a model and a touch similar to a human finger.

[Experimental Conditions]

Target type: Compression, Target Value: 10.0mm, Trigger Load: 1.0g, Test Speed: 1.0mm / s, Sample Temperature: 25 ± 3 ℃

The results are shown in Table 9 below.

Sample	Adhesiveness (mJ)
Comparative Example 3	0.12 ± 0.01
Example 12 (MP% 0.2%)	0.31 ± 0.02
Example 13 (MP% 2.0%)	0.72 ± 0.02
Example 14 (MP% 5.0%)	0.95 ± 0.01

As confirmed in Table 9, the adhesion of mascara of the example containing the hybrid mussel adhesive protein by concentration was very high compared to the formulation of Comparative Example 3 containing no.

Stability evaluation

The mascara of the above example in which mussel adhesive protein (MP) was added for each concentration In order to evaluate the stability, it was confirmed whether the phase separation for 8 weeks at 1 week intervals at 25 ℃.

As shown in Table 10 below, when the sample was observed at 25 ° C. for 8 weeks, phase separation was not confirmed, indicating stability.

	Comparative Example 3	Example 12	Example 13	Example 14
density	0%	0.2%	2.0%	5.0%
25 ℃	◎	◎	◎	◎

(◎: stable, △: abnormality sign, X: unstable)

Claims (4)

1. Makeup cosmetic composition containing a hybrid mussel adhesive protein.
2. According to claim 1, wherein the hybrid mussel adhesive protein makeup cosmetic composition, characterized in that consisting of the amino acid sequence of SEQ ID NO: 1.
3. The makeup cosmetic composition of claim 1, wherein the hybrid mussel adhesive protein is contained in an amount of 0.5 to 5 wt% based on the total weight of the cosmetic composition.
4. According to claim 1, wherein the makeup cosmetic composition is a makeup base, foundation, BB cream, concealer, lipstick, lip gloss, lip liner, powder, two-way cake, primer, pact, powder, eye shadow, blusher, eyeliner, eye mascara, Makeup cosmetic composition, characterized in that it has a formulation of hair gel, hair mascara or nail enamel.

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