WO2018004308A3 - 신규 내열성 과당-6-인산-3-에피머화 효소 및 이를 이용한 알룰로스 제조방법 - Google Patents

신규 내열성 과당-6-인산-3-에피머화 효소 및 이를 이용한 알룰로스 제조방법 Download PDF

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Publication number
WO2018004308A3
WO2018004308A3 PCT/KR2017/006985 KR2017006985W WO2018004308A3 WO 2018004308 A3 WO2018004308 A3 WO 2018004308A3 KR 2017006985 W KR2017006985 W KR 2017006985W WO 2018004308 A3 WO2018004308 A3 WO 2018004308A3
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WIPO (PCT)
Prior art keywords
epimerase
phosphate
fructose
same
producing allulose
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PCT/KR2017/006985
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English (en)
French (fr)
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WO2018004308A2 (ko
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양성재
조현국
이영미
김성보
조성준
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씨제이제일제당 (주)
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=60787012&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2018004308(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to AU2017287764A priority Critical patent/AU2017287764B2/en
Priority to PL17820584.5T priority patent/PL3480306T3/pl
Priority to JP2018567065A priority patent/JP6817343B2/ja
Priority to RU2019100788A priority patent/RU2733427C2/ru
Priority to ES17820584T priority patent/ES2950576T3/es
Application filed by 씨제이제일제당 (주) filed Critical 씨제이제일제당 (주)
Priority to MX2018016114A priority patent/MX2018016114A/es
Priority to BR112018077188-0A priority patent/BR112018077188B1/pt
Priority to EP17820584.5A priority patent/EP3480306B1/en
Priority to US16/314,067 priority patent/US10907182B2/en
Priority to CN201780041244.6A priority patent/CN109563499B/zh
Priority to CA3027687A priority patent/CA3027687C/en
Publication of WO2018004308A2 publication Critical patent/WO2018004308A2/ko
Publication of WO2018004308A3 publication Critical patent/WO2018004308A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01063—Polyphosphate--glucose phosphotransferase (2.7.1.63)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02002—Phosphoglucomutase (5.4.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

본 출원은 서열번호 1의 아미노산 서열로 이루어진 과당-6-인산-3-에피머화 효소 (fructose-6-phosphate-3-epimerase), 상기 과당-6-인산-3-에피머화 효소를 암호화하는 핵산 및 상기 핵산을 포함하는 형질전환체에 관한 것이다. 또한, 본 출원은 본 출원의 과당-6-인산-3-에피머화 효소를 포함하는 알룰로스(allulose) 생산용 조성물 및 본 출원의 과당-6-인산-3-에피머화 효소를 이용한 알룰로스 제조방법에 관한 것이다.
PCT/KR2017/006985 2016-06-30 2017-06-30 신규 내열성 과당-6-인산-3-에피머화 효소 및 이를 이용한 알룰로스 제조방법 WO2018004308A2 (ko)

Priority Applications (11)

Application Number Priority Date Filing Date Title
CA3027687A CA3027687C (en) 2016-06-30 2017-06-30 A novel thermostable fructose-6-phosphate-3-epimerase and a method for producing allulose using the same
BR112018077188-0A BR112018077188B1 (pt) 2016-06-30 2017-06-30 Método e composição compreendendo frutose-6-fosfato-3-epimerase termoestável para produzir alulose
JP2018567065A JP6817343B2 (ja) 2016-06-30 2017-06-30 新規な耐熱性のフルクトース−６−リン酸−３−エピメラーゼ及びそれを用いたアルロース製造方法
RU2019100788A RU2733427C2 (ru) 2016-06-30 2017-06-30 Новая термостабильная фруктозо-6-фосфат-3-эпимераза и способ получения аллюлозы с ее использованием
ES17820584T ES2950576T3 (es) 2016-06-30 2017-06-30 Fructosa-6-fosfato-3-epimerasa resistente al calor novedosa y método para producir alulosa mediante su uso
AU2017287764A AU2017287764B2 (en) 2016-06-30 2017-06-30 A Novel Thermostable Fructose-6-Phosphate-3-Epimerase and a Method for Producing Allulose Using the Same
MX2018016114A MX2018016114A (es) 2016-06-30 2017-06-30 Una novedosa fructosa-6-fosfato-3-epimerasa termoestable y un procedimiento para producir alulosa mediante el uso de la misma.
PL17820584.5T PL3480306T3 (pl) 2016-06-30 2017-06-30 Nowa, odporna na wysoką temperaturę fruktozo-6-fosforano-3-epimeraza i sposób wytwarzania allulozy z jej użyciem
EP17820584.5A EP3480306B1 (en) 2016-06-30 2017-06-30 Novel heat-resistant fructose-6-phosphate-3-epimerase and method for producing allulose by using same
US16/314,067 US10907182B2 (en) 2016-06-30 2017-06-30 Thermostable fructose-6-phosphate-3-epimerase and a method for producing allulose using the same
CN201780041244.6A CN109563499B (zh) 2016-06-30 2017-06-30 一种新型热稳定果糖-6-磷酸盐-3-差向异构酶和使用其生产阿洛糖的方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20160082543 2016-06-30
KR10-2016-0082543 2016-06-30

Publications (2)

Publication Number Publication Date
WO2018004308A2 WO2018004308A2 (ko) 2018-01-04
WO2018004308A3 true WO2018004308A3 (ko) 2018-02-22

Family

ID=60787012

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2017/006985 WO2018004308A2 (ko) 2016-06-30 2017-06-30 신규 내열성 과당-6-인산-3-에피머화 효소 및 이를 이용한 알룰로스 제조방법

Country Status (13)

Country Link
US (1) US10907182B2 (ko)
EP (1) EP3480306B1 (ko)
JP (1) JP6817343B2 (ko)
KR (2) KR101966530B1 (ko)
CN (1) CN109563499B (ko)
AU (1) AU2017287764B2 (ko)
BR (1) BR112018077188B1 (ko)
CA (1) CA3027687C (ko)
ES (1) ES2950576T3 (ko)
MX (1) MX2018016114A (ko)
PL (1) PL3480306T3 (ko)
RU (1) RU2733427C2 (ko)
WO (1) WO2018004308A2 (ko)

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BR112018077188B1 (pt) 2016-06-30 2022-09-06 Cj Cheiljedang Corporation Método e composição compreendendo frutose-6-fosfato-3-epimerase termoestável para produzir alulose
CN110088277B (zh) 2016-12-14 2024-10-11 博努莫斯有限责任公司 D-阿洛酮糖的酶法生产
CN108251468A (zh) * 2018-02-06 2018-07-06 南京朗奈生物技术有限公司 生物法生产d-阿洛酮糖的工艺
KR102233375B1 (ko) * 2018-12-11 2021-03-31 씨제이제일제당 주식회사 신규 싸이코스-6-인산의 탈인산화 효소, 이를 포함하는 싸이코스 생산용 조성물, 및 이를 이용한 싸이코스 제조방법
KR102138862B1 (ko) * 2019-03-08 2020-07-30 씨제이제일제당 주식회사 알룰로스를 생산하는 스태필로코커스 속 미생물 및 이를 이용한 알룰로스 제조방법
KR102256624B1 (ko) * 2019-03-19 2021-05-26 주식회사 삼양사 인산화당 에피머 효소
KR102080886B1 (ko) 2019-05-21 2020-02-24 씨제이제일제당 주식회사 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소
MX2021014409A (es) * 2019-05-31 2022-04-06 Bonumose Inc Produccion enzimatica de fructosa.
US20220372535A1 (en) * 2019-10-28 2022-11-24 Samyang Corporation Fructose-6-phosphate 3-epimerase and use thereof
CN114729345A (zh) * 2019-10-28 2022-07-08 株式会社三养社 果糖6-磷酸3-差向异构酶及其用途
KR102509561B1 (ko) * 2020-12-21 2023-03-14 주식회사 삼양사 높은 기질 특이성을 갖는 탈인산화 효소 단백질 및 이의 용도
CN114790469B (zh) * 2021-01-26 2024-03-26 中国科学院天津工业生物技术研究所 一种酶促合成阿洛酮糖的方法
WO2024189214A2 (de) 2023-03-15 2024-09-19 Annikki Gmbh Verfahren zur herstellung einer wässerigen lösung enthaltend l-psicose
EP4431614A1 (de) 2023-03-15 2024-09-18 Annikki GmbH Verfahren zur herstellung von wässerigen lösungen enthaltend d-psicose oder l-psicose
EP4446422A2 (de) 2023-03-15 2024-10-16 Annikki GmbH Verfahren zur herstellung einer wässerigen lösung enthaltend l-psicose

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Also Published As

Publication number Publication date
AU2017287764A1 (en) 2018-12-20
CN109563499B (zh) 2022-08-12
EP3480306B1 (en) 2023-06-07
JP6817343B2 (ja) 2021-01-20
RU2733427C2 (ru) 2020-10-01
MX2018016114A (es) 2019-10-14
CA3027687A1 (en) 2018-01-04
KR101966530B1 (ko) 2019-04-08
KR102042044B1 (ko) 2019-12-02
EP3480306A2 (en) 2019-05-08
US20190225997A1 (en) 2019-07-25
AU2017287764B2 (en) 2021-01-21
KR20190038775A (ko) 2019-04-09
PL3480306T3 (pl) 2023-11-06
RU2019100788A (ru) 2020-07-30
RU2019100788A3 (ko) 2020-07-30
CA3027687C (en) 2022-07-05
BR112018077188A2 (pt) 2019-06-25
CN109563499A (zh) 2019-04-02
US10907182B2 (en) 2021-02-02
ES2950576T3 (es) 2023-10-11
WO2018004308A2 (ko) 2018-01-04
KR20180004023A (ko) 2018-01-10
BR112018077188B1 (pt) 2022-09-06
JP2019522477A (ja) 2019-08-15
EP3480306A4 (en) 2020-03-11

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