WO2014202623A3 - Rasamsonia gene and use thereof - Google Patents

Rasamsonia gene and use thereof Download PDF

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Publication number
WO2014202623A3
WO2014202623A3 PCT/EP2014/062745 EP2014062745W WO2014202623A3 WO 2014202623 A3 WO2014202623 A3 WO 2014202623A3 EP 2014062745 W EP2014062745 W EP 2014062745W WO 2014202623 A3 WO2014202623 A3 WO 2014202623A3
Authority
WO
WIPO (PCT)
Prior art keywords
amino acid
acid sequence
seq
polypeptide
gene
Prior art date
Application number
PCT/EP2014/062745
Other languages
French (fr)
Other versions
WO2014202623A2 (en
Inventor
Alrik Pieter Los
Stefanus Cornelis Hendrikus Jozef Turk
Original Assignee
Dsm Ip Assets B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets B.V. filed Critical Dsm Ip Assets B.V.
Publication of WO2014202623A2 publication Critical patent/WO2014202623A2/en
Publication of WO2014202623A3 publication Critical patent/WO2014202623A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a polypeptide which comprises the amino acid sequence set out in SEQ ID NO: 1 or 17or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 3 or 19or a variant polypeptide, wherein the variant polypeptide (i) has at least 70% sequence identity with the sequence set out in SEQ ID NO: 1 or 17 or (ii) as an amino acid sequence that differs in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12 amino acids from the amino acid sequence of SEQ ID NO: 1 or 17. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes.
PCT/EP2014/062745 2013-06-19 2014-06-17 Rasamsonia gene and use thereof WO2014202623A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP13172726 2013-06-19
EP13172726.5 2013-06-19
EP13172760.4 2013-06-19
EP13172760 2013-06-19

Publications (2)

Publication Number Publication Date
WO2014202623A2 WO2014202623A2 (en) 2014-12-24
WO2014202623A3 true WO2014202623A3 (en) 2015-04-23

Family

ID=50980286

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2014/062745 WO2014202623A2 (en) 2013-06-19 2014-06-17 Rasamsonia gene and use thereof

Country Status (1)

Country Link
WO (1) WO2014202623A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2947152A1 (en) * 2014-05-21 2015-11-25 Clariant International Ltd. Process for the hydrolysis of lignocellulosic material, wherein the hydrolysate is used for microbial hydrolase production
CA2973302C (en) 2015-01-28 2022-10-25 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2017147690A1 (en) * 2016-03-02 2017-09-08 Valorbec Société en Commandite Engineered beta-glucosidases and methods of use thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010102982A1 (en) * 2009-03-10 2010-09-16 Dsm Ip Assets B.V. Method for improving the yield of a polypeptide
WO2011098577A1 (en) * 2010-02-11 2011-08-18 Dsm Ip Assets B.V. Host cell capable of producing enzymes useful for degradation of lignocellulosic material
WO2012000890A1 (en) * 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
US20130023014A1 (en) * 2010-03-31 2013-01-24 Meiji Seika Pharma Co., Ltd. Novel cellulase gene

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010102982A1 (en) * 2009-03-10 2010-09-16 Dsm Ip Assets B.V. Method for improving the yield of a polypeptide
WO2011098577A1 (en) * 2010-02-11 2011-08-18 Dsm Ip Assets B.V. Host cell capable of producing enzymes useful for degradation of lignocellulosic material
US20130023014A1 (en) * 2010-03-31 2013-01-24 Meiji Seika Pharma Co., Ltd. Novel cellulase gene
WO2012000890A1 (en) * 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
"ß-Glucosidases", vol. 533, 27 July 1993, AMERICAN CHEMICAL SOCIETY, Washington, DC, article TIM FOWLER: "Deletion of the Trichoderma reesei [beta]-Glucosidase Gene, bgl1", pages: 56 - 65, XP055137371, DOI: 10.1021/bk-1993-0533.ch005 *
COLLINS ET AL: "Molecular cloning and expression analysis of two distinct beta-glucosidase genes, bg1 and aven1, with very different biological roles from the thermophilic, saprophytic fungus Talaromyces emersonii", MYCOLOGICAL RESEARCH, ELSEVIER, GB, vol. 111, no. 7, 6 August 2007 (2007-08-06), pages 840 - 849, XP022188075, ISSN: 0953-7562, DOI: 10.1016/J.MYCRES.2007.05.007 *
DATABASE UniProt [online] 23 January 2007 (2007-01-23), "SubName: Full=Beta-glucosidase, putative;", XP002729154, retrieved from EBI accession no. UNIPROT:A1D1D3 Database accession no. A1D1D3 *
DEBORAH M WATERS ET AL: "Characterisation of a Talaromyces emersonii thermostable enzyme cocktail with applications in wheat dough rheology", ENZYME AND MICROBIAL TECHNOLOGY, STONEHAM, MA, US, vol. 49, no. 2, 1 July 2011 (2011-07-01), pages 229 - 236, XP002650558, ISSN: 0141-0229, [retrieved on 20110415], DOI: 10.1016/J.ENZMICTEC.2011.04.006 *
FEDOROVA NATALIE D ET AL: "Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus", PLOS GENETICS, PUBLIC LIBRARY OF SCIENCE, SAN FRANCISCO, CA, US, vol. 4, no. 4, 1 April 2008 (2008-04-01), XP002544950, ISSN: 1553-7390, DOI: 10.1371/JOURNAL.PGEN.1000046 *
MURRAY P ET AL: "Expression in Trichoderma reesei and characterisation of a thermostable family 3 beta-glucosidase from the moderately thermophilic fungus Talaromyces emersonii", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 38, no. 2, 1 December 2004 (2004-12-01), pages 248 - 257, XP004649879, ISSN: 1046-5928, DOI: 10.1016/J.PEP.2004.08.006 *

Also Published As

Publication number Publication date
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