KR102233375B1 - 신규 싸이코스-6-인산의 탈인산화 효소, 이를 포함하는 싸이코스 생산용 조성물, 및 이를 이용한 싸이코스 제조방법 - Google Patents

신규 싸이코스-6-인산의 탈인산화 효소, 이를 포함하는 싸이코스 생산용 조성물, 및 이를 이용한 싸이코스 제조방법 Download PDF

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KR102233375B1
KR102233375B1 KR1020180159351A KR20180159351A KR102233375B1 KR 102233375 B1 KR102233375 B1 KR 102233375B1 KR 1020180159351 A KR1020180159351 A KR 1020180159351A KR 20180159351 A KR20180159351 A KR 20180159351A KR 102233375 B1 KR102233375 B1 KR 102233375B1
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박현준
윤상영
조현국
양성재
김소형
김승환
박일향
손병삼
김성보
박승원
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씨제이제일제당 주식회사
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Priority to CA3122746A priority patent/CA3122746A1/en
Priority to PCT/KR2019/017114 priority patent/WO2020122504A1/ko
Priority to MX2021006808A priority patent/MX2021006808A/es
Priority to CN201980089137.XA priority patent/CN113544265B/zh
Priority to US17/312,697 priority patent/US20220056426A1/en
Priority to JP2021533165A priority patent/JP2022512197A/ja
Priority to BR112021011264-2A priority patent/BR112021011264A2/pt
Priority to EP19895174.1A priority patent/EP3882344A4/en
Priority to SG11202106032UA priority patent/SG11202106032UA/en
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Priority to IL283842A priority patent/IL283842A/en
Priority to CL2021001511A priority patent/CL2021001511A1/es
Priority to CONC2021/0008763A priority patent/CO2021008763A2/es
Priority to JP2022172294A priority patent/JP2023011749A/ja

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Abstract

본 출원은 싸이코스-6-인산의 탈인산화 효소, 이를 포함하는 미생물, 및 이를 이용한 싸이코스 제조방법에 관한 것이다.

Description

신규 싸이코스-6-인산의 탈인산화 효소, 이를 포함하는 싸이코스 생산용 조성물, 및 이를 이용한 싸이코스 제조방법 {A novel psicose-6-phosphatase, a composition for producing psicose comprising the same, and a method for producing psicose using the same}
본 출원은 싸이코스를 제조하기 위해 싸이코스-6-인산을 탈인산화 하는 신규 효소, 이를 포함하는 싸이코스 생산용 조성물, 및 이를 이용하여 싸이코스를 제조하는 방법에 관한 것이다.
싸이코스-3-에피머화효소(D-psicose-3-epimerase, EC 5.1.3.30) 및 타가토스-3-에피머화효소(D-tagatose-3-epimerase, EC 5.1.3.31)는 과당(D-fructose)을 3-에피머화(3-epimerization, 3번 탄소 에피머화)하여 싸이코스(D-psicose)를 생산할 수 있는 효소로 알려져 있다. 상기 효소를 이용하는 단일 효소반응으로 싸이코스를 생산하는 경우, 기질인 과당과 산물인 싸이코스 간에 일정 수준의 반응평형(reaction equilibrium)이 존재한다(산물/기질 = 약 20% 내지 35%). 따라서, 고순도의 싸이코스를 제조하기 위해서는 효소 반응 결과물로부터 비교적 고농도의 과당을 분리하여 제거해야 하는 추가 정제공정이 요구되는 문제점이 있다. 또한 과당은 전분이나 포도당에 비하여 비교적 고비용 원료로 과당을 원료로 생산시 싸이코스와 타가토스의 원가 비율이 높아지게 된다. 이에 비교적 경제적인 전분 또는 포도당을 이용한 반응을 통하여 알룰로스 및 타가토스 생산의 다양한 연구가 발표 되고 있다. (KR 10-2018-0004023, WO2018-112139, WO2017-059278, WO2018-129275).
한편, Chan 등(2008. Biochemistry. 47:9608-9617)은 과당-6-인산(D-fructose-6-phosphate) 및 싸이코스-6-인산(D-psicose-6-phosphate)에 대해서 3-에피머화 반응을 수행할 수 있는 스트렙토코커스 파이로제네스(Streptococcus pyogenes) 유래의 리불로스-5-인산-3-에피머화효소(D-ribulose-5-phosphate-3-epimerase, EC 5.1.3.1) 및 E. coli 유래의 싸이코스-6-인산-3-에피머화효소(D-psicose 6-phosphate-3-epimerase, EC 5.1.3.-)를 보고한 바 있으나, 상기 효소들은 내열성을 갖지 못하여 산업적으로 이용이 불가능한 문제점이 있다.
이러한 배경 하에, 본 발명자들은 경제적이면서 산업적으로 알룰로스로의 전환수율을 높일 수 있는 방법을 개발하기 위해 예의 노력하였다. 그 결과, 경제적 원료인 수크로스 또는 전분(예컨대, 말토덱스트린)을 싸이코스-6-인산으로 전환시킨 후, 상기 싸이코스-6-인산에 특이적이면서 비가역 반응경로를 수행하는 본 출원의 싸이코스-6-인산 탈인산화 효소(psicose-6-phosphate phosphatase)를 이용하여 싸이코스를 생산하는 경우, 싸이코스 생산 경로에 관여하는 복수의 효소를 동시에 사용 가능한 동시효소반응(one-pot enzymatic conversions)이 가능하고, 싸이코스 전환율을 현저히 높일 수 있음을 확인함으로써 본 출원을 완성하였다. 본 출원의 효소는 기존의 공지된 싸이코스-6-인산의 탈인산화 효소(Panoramic view of a superfamily of phosphatases through substrate profiling, PNAS, 06.04. 2015, Huang 등)에 비해 싸이코스 생산에 있어 보다 적합한 이점을 갖는다.
본 출원의 하나의 목적은 싸이코스-6-인산의 탈인산화 효소를 제공하는 것이다.
본 출원의 다른 하나의 목적은 싸이코스-6-인산의 탈인산화 효소를 코딩하는 핵산을 제공하는 것이다.
본 출원의 또 다른 하나의 목적은 싸이코스-6-인산의 탈인산화 효소를 코딩하는 핵산을 포함하는 형질전환체를 제공하는 것이다.
본 출원의 또 다른 하나의 목적은 싸이코스-6-인산 탈인산화 효소, 이를 발현하는 미생물, 또는 상기 미생물의 배양물을 포함하는 싸이코스 생산용 조성물을 제공하는 것이다.
본 출원의 또 다른 하나의 목적은 싸이코스-6-인산 탈인산화 효소, 이를 발현하는 미생물, 또는 상기 미생물의 배양물을 싸이코스-6-인산과 접촉시켜 싸이코스-6-인산을 싸이코스로 전환하는 단계를 포함하는 싸이코스 제조방법을 제공하는 것이다.
이하, 본 출원 내용에 대하여 구체적으로 설명하면 다음과 같다. 한편, 본 출원에서 개시한 일 양태의 설명 및 실시형태는 공통된 사항에 대하여 다른 양태의 설명 및 실시 형태에도 적용될 수 있다. 또한, 본 출원에서 개시된 다양한 요소들의 모든 조합이 본 출원의 범주에 속한다. 더불어, 하기 기술된 구체적인 서술에 의하여 본 출원의 범주가 제한된다고 볼 수 없다.
상기 목적을 달성하기 위한 본 출원의 하나의 양태는 싸이코스-6-인산의 탈인산화 효소를 제공하는 것이다.
구체적으로, 본 출원의 싸이코스-6-인산의 탈인산화 효소는 아리사이크로바실러스(Alicyclobacillus), 아미코라톱시스(Amycolatopsis), 아나이로릴네아(Anaerolinea), 아르케오글로부스(Archaeoglobus), 바실러스(Bacillus), 칼디셀룰로시럽터(Caldicellulosiruptor), 칼디리테아(Caldilinea), 칼디트릭스(Caldithrix), 카르보식도셀라(Carboxydocella), 카르복시도테르무스(Carboxydothermus), 클로로플렉시(Chloroflexi), 데플루비토가(Defluviitoga), 데이노코커스(Deinococcus), 데술푸로콕쿠스 (Desulfurococcus), 딕티오글로무스(Dictyoglomus), 에푸시바실러스(Effusibacillus), 페르비도박테리움(Fervidobacterium), 게오바실러스(Geobacillus), 할로코커스(Halococcus), 하이드로지니비르가(Hydrogenivirga), 하이드로지노박터(Hydrogenobacter), 히페르써무스 (Hyperthermus), 코스모토가(Kosmotoga), 마리니토가(Marinitoga), 메이오써무스(Meiothermus), 메소토가(Mesotoga), 메탈로스파에라(Metallosphaera), 메타노셀라(Methanocella), 메타노코코이데스(Methanococcoides), 메타노하로비움(Methanohalobium), 메타노로부스(Methanolobus), 메타노사르키나(Methanosarcina), 메타노써무스(Methanothermus), 페트로토가(Petrotoga), 피크로필러스(Picrophilus), 슈도노카르디아(Pseudonocardia), 피로코커스(Pyrococcus), 피로딕티움(Pyrodictium), 로도써무스(Rhodothermus), 슬라키아(Slackia), 스타피로써무스(Staphylothermus), 설포로버스(Sulfolobus), 써마나에로트릭스(Thermanaerothrix), 써모아네로박터 (Thermoanaerobacter), 써모아네로박테리엄(Thermoanaerobacterium), 써모비피다(Thermobifida), 써모코카스 (Thermococcus), 써모크리니스 (Thermocrinis), 써모프렉서스(Thermoflexus), 써모토가(Thermotoga), 써머스(Thermus), 또는 트루페라(Truepera) 속 유래의 싸이코스-6-인산의 탈인산화 효소일 수 있고, 보다 구체적으로, 아리사이크로바실러스 아시도칼다리우스(Alicyclobacillus acidocaldarius), 아리사이크로바실러스 텡촌젠시스(Alicyclobacillus tengchongensis), 아미코라톱시스 써모플라바(Amycolatopsis thermoflava), 아나이로릴네아 써모리모사(Anaerolinea thermolimosa), 아나이로릴네아 써모필라(Anaerolinea thermophila), 아르케오글로부스 푸지두스(Archaeoglobus fugidus), 아르케오글로부스 프로펀두스(Archaeoglobus profundus), 아르케오글로부스 베네피커스(Archaeoglobus veneficus), 바실러스 리체니포르미스(Bacillus licheniformis), 칼디셀룰로시럽터 베스키(Caldicellulosiruptor bescii), 칼디리네아 아에로필라(Caldilinea aerophila), 칼디트릭스 아비시(Caldithrix abyssi), 카르보식도셀라 sp. ULO1(Carboxydocella sp. ULO1), 카르복시도테르무스 페리레두센스(Carboxydothermus ferrireducens), 클로로플렉시 박테리움 54-19(Chloroflexi bacterium 54-19), 데플루비토가 투니시엔시스(Defluviitoga tunisiensis), 데이노코커스 아에리우스(Deinococcus aerius), 데이노코커스 아파첸시스(Deinococcus apachensis), 데이노코커스 아카틸리스(Deinococcus aquatilis), 데이노코커스 지오써르말리스(Deinococcus geothermalis), 데이노코커스 호피엔시스(Deinococcus hopiensis), 데이노코커스 마리코펜시스(Deinococcus maricopensis), 데이노코커스 무라이(Deinococcus murrayi), 데이토코커스 레티큘리테르미티스(Deinococcus reticulitermitis), 데이토코커스 울루무퀴엔시스(Deinococcus wulumuqiensis), 데이토코커스 sp. Leaf326(Deinococcus sp. Leaf326), 데이토코커스 포에니시스(Deinococcus phoenicis), 데이노코커스 프로테오라이티커스(Deinococcus proteolyticus), 데이노코커스 sp. 17bor-2(Deinococcus sp. 17bor-2), 데이노코커스 sp. NW-56(Deinococcus sp. NW-56), 데이노코커스 sp. RL(Deinococcus sp. RL), 데이노코커스 sp. YIM 77859(Deinococcus sp. YIM 77859), 데술푸로콕쿠스 무코서스(Desulfurococcus mucosus), 딕티오글로무스 투르지둠(Dictyoglomus turgidum), 에푸시바실러스 폴리애(Effusibacillus pohliae), 페르비도박테리움 곤드와넨스(Fervidobacterium gondwanense), 페르비도박테리움 이스란디쿰(Fervidobacterium islandicum), 페르비도박테리움 노도섬(Fervidobacterium nodosum), 페르비도박테리움 페니보란스(Fervidobacterium pennivorans), 게오바실러스 sp.(Geobacillus sp.), 게오바실러스 스테아로써모필러스(Geobacillus stearothermophilus), 할로코커스 사리포디내(Halococcus salifodinae), 하이드로지니비르가 sp. 128-5-R1-1(Hydrogenivirga sp. 128-5-R1-1), 하이드로지노박터 하이드로제노필러스(Hydrogenobacter hydrogenophilus), 하이드로지노박터 써모필러스(Hydrogenobacter thermophilus), 히페르테르무스 부티리커스(Hyperthermus butylicus), 코스모토가 아레니코랄리나(Kosmotoga arenicorallina), 코스모토가 오레아리아(Kosmotoga olearia), 마리니토가 피에조필라(Marinitoga piezophila), 메이오써무스 세르베레우스(Meiothermus cerbereus), 메이오써무스 크리아로필러스(Meiothermus chliarophilus), 메이오써무스 루베르(Meiothermus ruber), 메이오써무스 실바누스(Meiothermus Silvanus), 메이오써무스 타이와넨시스(Meiothermus taiwanensis), 메이오써무스 티미두스(Meiothermus timidus), 메이오써무스 루푸스(Meiothermus rufus), 메소토가 인페라(Mesotoga infera), 메탈로스파에라 세둘라(Metallosphaera sedula), 메타노셀라 콘라디(Methanocella conradii), 메타노코코이데스 메틸루텐스(Methanococcoides methylutens), 메타노하로비움 에베스티가툼(Methanohalobium evestigatum), 메타노로부스 틴다리우스(Methanolobus tindarius), 메타노사르키나 시키리아(Methanosarcina sicilia), 메타노써무스 페르비두스(Methanothermus fervidus), 페트로토가 모빌리스(Petrotoga mobilis), 피크로필러스 토리두스(Picrophilus torridus), 슈도노카르디아 써모필리아(Pseudonocardia thermophila), 피로코커스 퓨리오서스(Pyrococcus furiosus), 피로딕티움 오쿨텀(Pyrodictium occultum), 로도써무스 마리누스(Rhodothermus marinus), 슬라키아 헤리오트리니레두센스(Slackia heliotrinireducens), 스타피로써무스 마리너스(Staphylothermus marinus), 설포로버스 아시도칼다리우스(Sulfolobus acidocaldarius), 써마나에로트릭스 닥센시스(Thermanaerothrix daxensis), 써모아네로박터 sp.(Thermoanaerobacter sp.), 써모아네로박터 써모하이드로설퍼리쿠스(Thermoanaerobacter thermohydrosulfuricus), 써모아네로박터 비에게리(Thermoanaerobacter wiegelii), 써모아네로박테리움 질라노리티쿰(Thermoanaerobacterium xylanolyticum), 써모비피다 하로토레란스(Thermobifida halotolerans), 써모코카스 세레르(Thermococcus celer), 써모코카스 리토라리스(Thermococcus litoralis), 써모코카스 프로폰두스(Thermococcus profundus), 써모크리니스 미네르배(Thermocrinis minervae), 써모크리니스 루베르(Thermocrinis ruber), 써모프렉서스 후겐홀트지(Thermoflexus hugenholtzii), 써모토가 레틴가애(Thermotoga lettingae), 써모토가 네아포리타나(Thermotoga neapolitana), 써모토가 페트로필리아(Thermotoga petrophilia), 써머스 아미로리케파시엔스(Thermus amyloliquefaciens), 써머스 필리포르미스(Thermus filiformis), 써머스 써모필러스(Thermus thermophilus), 또는 트루페라 라디오빅트릭스(Truepera radiovictrix) 유래인 것일 수 있으나, 이에 제한되지 않는다.
본 출원에서, “싸이코스-6-인산(psicose-6-phosphate)”은 알룰로스-6-인산(allulose　6-phosphate)로도 알려져 있으며, “싸이코스-6-인산의 탈인산화 효소”는 싸이코스-6-인산의 인산기를 탈인산화시켜 싸이코스를 생산하는 작용을 하는 효소를 의미한다.
본 출원의 싸이코스-6-인산의 탈인산화 효소는 고농도 전분을 분해하여 싸이코스를 제조함에 있어서, 전분 가공 효소들 및 인산당 전환 효소들과 복합적으로 조합하여 고효율로 싸이코스를 제조하는데 이용될 수 있다.
본 출원의 싸이코스-6-인산 탈인산화 효소는 서열번호 1 내지 222 중 어느 하나의 아미노산 서열이거나, 또는 상기 아미노산 서열과 70% 이상 동일성을 갖는 아미노산 서열을 포함하는 것일 수 있다.
또한, 서열번호 1, 6, 9, 12, 26, 29,38 내지 43, 45 내지 53,56, 57,59, 60, 64 내지 66, 69, 70, 72, 76, 80, 81, 91 내지 93, 95, 99 내지 103, 113, 114, 116,117, 131, 134, 136, 142, 145, 146, 148, 164, 167, 169, 172, 177, 184 내지 187, 189, 191, 192, 211, 217, 및 221 중 어느 하나의 아미노산 서열이거나, 또는 상기 아미노산 서열과 70% 이상 동일성을 갖는 아미노산 서열을 포함하는 것 일 수 있으나, 이에 제한되지 않는다.
보다 구체적으로는, 서열번호 26, 29, 53, 56, 60, 70, 76, 80, 81, 116, 117, 131, 134, 145, 167, 185, 186, 및 191 중 어느 하나의 아미노산 서열이거나, 또는 상기 아미노산 아미노산 서열과 70% 이상 동일성을 갖는 아미노산 서열을 포함하는 것 일 수 있으나, 이에 제한되지 않는다.
또한, 상기 아미노산 서열과 동일한 활성을 갖는 서열은 제한없이 포함될 수 있다. 또한, 서열번호 1 내지 222 중 어느 하나의 아미노산 서열 또는 이와 80% 이상의 상동성(homology) 또는 동일성(identity)을 갖는 아미노산 서열을 포함할 수 있으나, 이에 제한되는 것은 아니다. 구체적으로 상기 아미노산은 서열번호 1 내지 222의 서열 및 상기 서열과 적어도 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 또는 99% 이상의 상동성 또는 동일성을 가지는 아미노산을 포함할 수 있다. 또한, 이러한 상동성 또는 동일성을 가지며 상기 단백질에 상응하는 효능을 나타내는 아미노산 서열이라면, 일부 서열이 결실, 변형, 치환 또는 부가된 아미노산 서열을 갖는 단백질도 본 출원의 범위내에 포함됨은 자명하다.
즉, 본 출원에서 '특정 서열번호로 기재된 아미노산 서열을 갖는 단백질 '이라고 기재되어 있다 하더라도, 해당 서열번호의 아미노산 서열로 이루어진 단백질과 동일 혹은 상응하는 활성을 가지는 경우라면, 일부 서열이 결실, 변형, 치환, 보존적 치환 또는 부가된 아미노산 서열을 갖는 단백질도 본 출원에서 사용될 수 있음은 자명하다. 예를 들어, 상기 효소와 동일 혹은 상응하는 활성을 가지는 경우라면 상기 아미노산 서열 앞뒤에 단백질의 기능을 변경하지 않는 서열 추가, 자연적으로 발생할 수 있는 돌연변이, 이의 잠재성 돌연변이 (silent mutation) 또는 보존적 치환을 제외하는 것이 아니며, 이러한 서열 추가 혹은 돌연변이를 가지는 경우에도 본 출원의 범위 내에 속하는 것이 자명하다.
본 출원에서 용어, '상동성(homology)' 또는 '동일성(identity)'은 두 개의 주어진 아미노산 서열 또는 염기 서열과 서로 관련된 정도를 의미하며 백분율로 표시될 수 있다.
용어 상동성 및 동일성은 종종 상호교환적으로 이용될 수 있다.
보존된 (conserved) 폴리뉴클레오티드 또는 폴리펩티드의 서열 상동성 또는 동일성은 표준 배열 알고리즘에 의해 결정되며, 사용되는 프로그램에 의해 확립된 디폴트 갭 페널티가 함께 이용될 수 있다. 실질적으로, 상동성을 갖거나 (homologous) 또는 동일한 (identical) 서열은 중간 또는 높은 엄격한 조건(stringent conditions)에서 일반적으로 서열 전체 또는 전체-길이의 적어도 약 50%, 60%, 70%, 80% 또는 90% 이상으로 하이브리드할 수 있다. 하이브리드화는 폴리뉴클레오티드에서 코돈 대신 축퇴 코돈을 함유하는 폴리뉴클레오티드 또한 고려된다.
임의의 두 폴리뉴클레오티드 또는 폴리펩티드 서열이 상동성, 유사성 또는 동일성을 갖는지 여부는 예를 들어, Pearson et al (1988)[Proc. Natl. Acad. Sci. USA 85]: 2444에서와 같은 디폴트 파라미터를 이용하여 "FASTA" 프로그램과 같은 공지의 컴퓨터 알고리즘을 이용하여 결정될 수 있다. 또는, EMBOSS 패키지의 니들만 프로그램(EMBOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000, Trends Genet. 16: 276-277)(버전 5.0.0 또는 이후 버전)에서 수행되는 바와 같은, 니들만-운치(Needleman-Wunsch) 알고리즘(Needleman and Wunsch, 1970, J. Mol. Biol. 48: 443-453)이 사용되어 결정될 수 있다. (GCG 프로그램 패키지 (Devereux, J., et al, Nucleic Acids Research 12: 387 (1984)), BLASTP, BLASTN, FASTA (Atschul, [S.] [F.,] [ET AL, J MOLEC BIOL 215]: 403 (1990); Guide to Huge Computers, Martin J. Bishop, [ED.,] Academic Press, San Diego,1994, 및 [CARILLO ETA/.](1988) SIAM J Applied Math 48: 1073을 포함한다). 예를 들어, 국립 생물공학 정보 데이터베이스 센터의 BLAST, 또는 ClustalW를 이용하여 상동성, 유사성 또는 동일성을 결정할 수 있다.
폴리뉴클레오티드 또는 폴리펩티드의 상동성, 유사성 또는 동일성은 예를 들어, Smith and Waterman, Adv. Appl. Math (1981) 2:482에 공지된 대로, 예를 들면, Needleman et al. (1970), J Mol Biol.48: 443과 같은 GAP 컴퓨터 프로그램을 이용하여 서열 정보를 비교함으로써 결정될 수 있다. 요약하면, GAP 프로그램은 두 서열 중 더 짧은 것에서의 기호의 전체 수로, 유사한 배열된 기호(즉, 뉴클레오티드 또는 아미노산)의 수를 나눈 값으로 정의한다. GAP 프로그램을 위한 디폴트 파라미터는 (1) 일진법 비교 매트릭스(동일성을 위해 1 그리고 비-동일성을 위해 0의 값을 함유함) 및 Schwartz and Dayhoff, eds., Atlas Of Protein Sequence And Structure, National Biomedical Research Foundation, pp. 353-358 (1979)에 의해 개시된 대로, Gribskov et al(1986) Nucl. Acids Res. 14: 6745의 가중된 비교 매트릭스 (또는 EDNAFULL(NCBI NUC4.4의 EMBOSS 버전) 치환 매트릭스); (2) 각 갭을 위한 3.0의 페널티 및 각 갭에서 각 기호를 위한 추가의 0.10 페널티 (또는 갭 개방 패널티 10, 갭 연장 패널티 0.5); 및 (3) 말단 갭을 위한 무 페널티를 포함할 수 있다. 따라서, 본 출원에서 사용된 것으로서, 용어 "상동성" 또는 "동일성"은 서열들간의 관련성(relevance)를 나타낸다.
본 출원의 싸이코스-6-인산 탈인산화 효소는 싸이코스-6-인산에 선택적으로 탈인산화 반응을 하는 효소일 수 있다. 구체적으로 싸이코스-6-인산 탈인산화 효소는 싸이코스-6-인산과 포도당-1-인산(D-glucose-1-phosphate), 포도당-6-인산(D-glucose-6-phosphate) 또는 과당-6-인산(D-fructose-6-phosphate)을 혼합하였을 시 싸이코스-6-인산에 탈인산화 반응을 하는 효소일 수 있다. 일례로 싸이코스-6-인산 탈인산화 효소는 동량의 싸이코스-6-인산, 포도당-1-인산, 포도당-6-인산, 및 과당-6-인산을 혼합하였을 때 싸이코스-6-인산의 탈인산화율이 1% 이상, 10% 이상, 또는 30% 이상일 수 있다. 이와 같은 본 출원의 싸이코스-6-인산 탈인산효소의 선택적 활성에 기인하여 복수의 효소 및 기질을 동시에 사용하는 동시효소반응(one-pot enzymatic conversion)에서 높은 싸이코스 전환율을 나타낼 수 있다.
본 출원의 상기 효소 자체 또는 이를 발현하는 DNA를 균주에 형질전환시키고, 이를 배양하여 배양물을 수득하고, 상기 배양물을 파쇄하여, 컬럼 등을 통해 정제한 것일 수 있다. 상기 형질전환용 균주로는 대장균(Escherichia coli), 코리네박테리움 글루타미쿰(Corynebacterum glutamicum), 아스퍼질러스 오리제(Aspergillus oryzae), 사카로마이세스 세레비지에(Saccharomyces cerevisiae), 야로위아 리폴리티카(Yarrowia lipolytica), 피키아 패스토리스(Pichia pastoris)또는 바실러스 섭틸리스(Bacillus subtilis)가 있으나, 이에 제한되지 않으며, 향 후 GRAS(Generally Recognized as Safe) 균주 내 형질 전환 가능성이 있을 수 있다.
본 출원의 다른 하나의 양태는 상기 싸이코스-6-인산의 탈인산화 효소를 코딩하는 핵산, 또는 상기 핵산을 포함하는 벡터를 제공하는 것이다.
본 출원에서, "핵산"은 DNA 또는 RNA 분자를 포괄적으로 포함하는 의미를 가지며, 핵산에서 기본 구성 단위인 뉴클레오타이드는 천연 뉴클레오타이드뿐만 아니라, 당 또는 염기 부위가 변형된 유사체도 포함할 수 있다(참조문헌: Scheit, Nucleotide Analogs, John Wiley, New York(1980); Uhlman 및 Peyman, Chemical Reviews, 90:543-584(1990)).
본 출원의 효소를 코딩하는 핵산은 단위체인 뉴클레오티드가 공유결합에 의해 연결된 DNA 또는 RNA 서열일 수 있고, 구체적으로, 서열번호 1 부터 222의 아미노산 서열의 DNA화 전환시(아미노산을 61개 codon으로 변형)에 가능한 모든 가지 수 중 어느 하나의 뉴클레오티드 서열일 수 있고, 보다 구체적으로 본 출원의 서열번호 1 내지 222의 아미노산 서열 중 어느 하나의 아미노산 서열로 번역될 수 있는 각각의 뉴클레오티드와 90% 이상, 95% 이상, 97% 이상, 99% 이상 또는 100%의 상동성, 유사성 또는 동일성을 가지면서, 번역되어 목적하는 효소 활성을 나타낼 수 있는 핵산을 포함할 수 있다. 코돈 축퇴성(codon degeneracy)에 의해 활성이 동일한 단백질, 번역(Transcripstion) 후 아미노산 서열이 동일한, 구체적으로 서열번호 1 내지 222중 어느 하나의 아미노산 서열로 이루어진 단백질 또는 이와 상동성, 유사성, 또는 동일성을 가지는 단백질로 번역될 수 있는 폴리뉴클레오티드 역시 본 출원의 범위에 포함될 수 있음은 자명하다. 보다 구체적으로, 본 출원의 핵산은 서열은 별도 표기하지 않으며, 서열번호 1 내지 222 아미노산 서열로 번역(translation) 가능한 모든 DNA 코돈 가지수로 이루어진 것일 수 있으나, 이에 제한되지 않는다.
또한 공지의 유전자 서열로부터 조제될 수 있는 프로브, 예를 들면, 상기 염기 서열의 전체 또는 일부에 대한 상보 서열과 엄격한 조건 하에 하이드리드화하여, 본 출원의 효소를 코딩하는 서열이라면 제한없이 포함될 수 있다.
상기 "엄격한 조건(stringent condition)"이란 폴리뉴클레오티드 간의 특이적 혼성화를 가능하게 하는 조건을 의미한다. 이러한 조건은 문헌 (예컨대, J. Sambrook et al., 상동)에 구체적으로 기재되어 있다. 예를 들어, 상동성(homology) 또는 동일성(identity)이 높은 유전자끼리, 80% 이상, 85% 이상, 구체적으로는 90% 이상, 보다 구체적으로는 95% 이상, 더욱 구체적으로는 97% 이상, 특히 구체적으로는 99% 이상의 상동성 또는 동일성을 갖는 유전자끼리 하이브리드화하고, 그보다 상동성 또는 동일성이 낮은 유전자끼리 하이브리드화하지 않는 조건, 또는 통상의 써던 하이브리드화(southern hybridization)의 세척 조건인 60℃, 1 X SSC, 0.1% SDS, 구체적으로는 60℃, 0.1 X SSC, 0.1% SDS, 보다 구체적으로는 68℃, 0.1 X SSC, 0.1% SDS에 상당하는 염 농도 및 온도에서, 1회, 구체적으로는 2회 내지 3회 세정하는 조건을 열거할 수 있다.
혼성화는 비록 혼성화의 엄격도에 따라 염기 간의 미스매치 (mismatch)가 가능할지라도, 두 개의 핵산이 상보적 서열을 가질 것을 요구한다. 용어, "상보적"은 서로 혼성화가 가능한 뉴클레오티드 염기 간의 관계를 기술하는데 사용된다. 예를 들면, DNA에 관하여, 아데노신은 티민에 상보적이며 시토신은 구아닌에 상보적이다. 따라서, 본 출원은 또한 실질적으로 유사한 핵산 서열뿐만 아니라 전체 서열에 상보적인 단리된 핵산 단편을 포함할 수 있다.
구체적으로, 상동성 또는 동일성을 가지는 폴리뉴클레오티드는 55 ℃의 Tm 값에서 혼성화 단계를 포함하는 혼성화 조건을 사용하고 상술한 조건을 사용하여 탐지할 수 있다. 또한, 상기 Tm 값은 60 ℃, 63 ℃ 또는 65 ℃일 수 있으나, 이에 제한되는 것은 아니고 그 목적에 따라 당업자에 의해 적절히 조절될 수 있다.
폴리뉴클레오티드를 혼성화하는 적절한 엄격도는 폴리뉴클레오티드의 길이 및 상보성 정도에 의존하고 변수는 해당기술분야에 잘 알려져 있다(Sambrook et al., supra, 9.50-9.51, 11.7-11.8 참조).
본 출원에서, "벡터"는 적합한 숙주 내에서 목적 변이형 단백질을 발현시킬 수 있도록 적합한 조절 서열에 작동 가능하게 연결된 본 출원의 효소를 코딩하는 핵산의 염기서열을 함유하는 DNA 제조물을 의미한다. 상기 조절 서열은 전사를 개시할 수 있는 프로모터, 그러한 전사를 조절하기 위한 임의의 오퍼레이터 서열, 적합한 mRNA 리보좀 결합부위를 코딩하는 서열, 및 전사 및 해독의 종결을 조절하는 서열을 포함할 수 있다. 벡터는 적당한 숙주세포 내로 형질전환된 후, 숙주 게놈과 무관하게 복제되거나 기능할 수 있으며, 게놈 그 자체에 통합될 수 있다.
본 출원에서 사용되는 벡터는 숙주세포 내에서 복제 가능한 것이면 특별히 한정되지 않으며, 당업계에 알려진 임의의 벡터를 이용할 수 있다. 통상 사용되는 벡터의 예로는 천연 상태이거나 재조합된 상태의 플라스미드, 코스미드, 바이러스 및 박테리오파지를 들 수 있다. 예를 들어, 파지 벡터 또는 코스미드 벡터로서 pWE15, M13, MBL3, MBL4, IXII, ASHII, APII, t10, t11, Charon4A, 및 Charon21A 등을 사용할 수 있으며, 플라스미드 벡터로서 pBR계, pUC계, pBluescriptII계, pGEM계, pTZ계, pCL계 및 pET계 등을 사용할 수 있다. 구체적으로는 pDZ, pACYC177, pACYC184, pCL, pECCG117, pUC19, pBR322, pMW118, pCC1BAC 벡터 등을 사용할 수 있다.
본 출원은 또 다른 양태는 본 출원의 각 효소를 코딩하는 핵산 또는 본 출원의 효소를 코딩하는 핵산을 포함하는 벡터를 포함하는 형질전환체를 제공한다.
본 출원에서, "효소를 코딩하는 핵산을 포함하는 형질전환체" 또는 "효소를 코딩하는 핵산을 포함하는 벡터를 포함하는 형질전환체"는, 본 출원의 싸이코스-6-인산 탈인산화 효소가 발현되도록 재조합된 미생물을 의미할 수 있다. 예를 들어 싸이코스-6-인산 탈인산화 효소를 코딩하는 핵산을 포함하거나, 또는 싸이코스-6-인산 탈인산화 효소를 코딩하는 핵산을 포함하는 벡터로 형질전환되어 상기 싸이코스-6-인산 탈인산화 효소를 발현할 수 있는 숙주세포 또는 미생물을 의미한다. 본 출원의 목적상, 상기 형질전환체가 발현하는 싸이코스-6-인산 탈인산화 효소는 서열번호 1 내지 222 중 어느 하나의 아미노산 서열로 구성된 것일 수 있으나, 이에 제한되지 않는다.
본 출원에서 용어 "형질전환"은 본 출원의 싸이코스-6-인산의 탈인산화 효소를 코딩하는 핵산을 포함하는 벡터를 숙주세포 내에 도입하여 숙주세포 내에서 상기 핵산이 암호화하는 단백질이 발현할 수 있도록 하는 것을 의미한다. 형질전환된 핵산은 숙주세포 내에서 발현될 수 있기만 한다면, 숙주세포의 염색체 내에 삽입되어 위치하거나 염색체 외에 위치하거나 상관없이 이들 모두를 포함할 수 있다. 또한, 상기 핵산은 본 출원의 싸이코스-6-인산의 탈인산화 효소를 코딩하는 핵산을 암호화하는 DNA 및 RNA를 포함한다. 상기 핵산은 숙주세포 내로 도입되어 발현될 수 있는 것이면, 어떠한 형태로 도입되는 것이든 상관없다. 예를 들면, 상기 핵산은 자체적으로 발현되는데 필요한 모든 요소를 포함하는 유전자 구조체인 발현 카세트(expression cassette)의 형태로 숙주세포에 도입될 수 있다. 상기 발현 카세트는 통상 상기 핵산에 작동 가능하게 연결되어 있는 프로모터 (promoter), 전사 종결신호, 리보좀 결합부위 및 번역 종결신호를 포함할 수 있다. 상기 발현 카세트는 자체 복제가 가능한 발현 벡터 형태일 수 있다. 또한, 상기 핵산은 그 자체의 형태로 숙주세포에 도입되어 숙주세포에서 발현에 필요한 서열과 작동 가능하게 연결되어 있는 것일 수도 있으며, 이에 한정되지 않는다.
또한, 상기에서 용어 "작동 가능하게 연결"된 것이란 본 출원의 싸이코스-6-인산의 탈인산화 효소를 코딩하는 핵산의 전사를 개시 및 매개하도록 하는 프로모터 서열과 상기 유전자 서열이 기능적으로 연결되어 있는 것을 의미한다.
상기 핵산 또는 벡터의 염색체 내로의 삽입은 당업계에 알려진 임의의 방법, 예를 들면, 상동 재조합(homologous recombination)에 의하여 이루어질 수 있으나, 이에 제한되지는 않는다. 상기 염색체 삽입 여부를 확인하기 위한 선별 마커(selection marker)를 추가로 포함할 수 있다. 선별 마커는 벡터로 형질전환된 세포를 선별, 즉 목적 핵산 분자의 삽입 여부를 확인하기 위한 것으로, 약물 내성, 영양 요구성, 세포 독성제에 대한 내성 또는 표면 변이형 단백질의 발현과 같은 선택가능 표현형을 부여하는 마커들이 사용될 수 있다. 선택제(selective agent)가 처리된 환경에서는 선별 마커를 발현하는 세포만 생존하거나 다른 표현 형질을 나타내므로, 형질전환된 세포를 선별할 수 있다.
본 출원의 벡터를 형질전환 시키는 방법은 핵산을 세포 내로 도입하는 어떤 방법도 포함되며, 숙주세포에 따라 당 분야에서 공지된 바와 같이 적합한 표준 기술을 선택하여 수행할 수 있다. 예를 들어, 전기천공법(electroporation), 인산칼슘(CaPO4) 침전, 염화칼슘(CaCl2) 침전, 레트로바이러스 감염(retroviral infection), 미세주입법(microinjection), 폴리에틸렌글리콜(PEG)법, DEAE-덱스트란법, 양이온 리포좀법, 및 초산 리튬-DMSO법 등이 있으나, 이에 제한되지 않는다.
상기 숙주 세포로는 DNA의 도입효율이 높고, 도입된 DNA의 발현 효율이 높은 숙주를 사용하는 것이 좋은데, 예를 들어 코리네속 미생물, 에스케리키아속 미생물, 세라티아속 미생물, 바실러스속 미생물, 사카로마이시스세레비제 속 미생물 또는 피키아속 미생물일 수 있고, 구체적으로 대장균(E. coli)일 수 있으나, 이에 제한되는 것은 아니며, GRAS 균주 모두에 적용 가능 하다.
보다 구체적으로, 본 출원의 형질전환체는 E. coli BL21(DE3)/pET-CJ-ap1 ~ E. coli BL21(DE3)/pET-CJ-ap222로 총 222개이다.
본 출원의 또 다른 하나의 양태는 싸이코스-6-인산 탈인산화 효소, 이를 발현하는 미생물, 또는 상기 미생물의 배양물을 포함하는 싸이코스 생산용 조성물을 제공하는 것이다. 구체적으로, 상기 조성물은 인산당을 추가로 포함할 수 있고, 보다 구체적으로, 싸이코스-6-인산을 기질로서 추가로 포함하는 것일 수 있으나, 이에 제한되지 않는다.
본 출원의 싸이코스 생산용 조성물은 싸이코스-6-인산을 탈인산화하여 싸이코스를 생산하는 작용을 하는 싸이코스-6-인산 탈인산화 효소, 상기 효소를 발현하는 미생물, 또는 상기 효소를 포함하는 미생물의 배양물을 포함함으로써, 싸이코스-6-인산의 탈인산화를 통해 싸이코스를 제조하는 것일 수 있다.
또한, 본 출원의 싸이코스 생산용 조성물은, 본 출원의 싸이코스 제조 경로에 관여하는 효소 및/또는 기질[(i) 전분, 말토덱스트린, 수크로스 또는 이의 조합; (ii) 포스페이트(phosphate) 또는 폴리포스페이트(polyphosphate) 또는 기타 인산화 화합물; (iii) 과당-6-인산-3-에피머화 효소; (iv) 포도당-6-인산-이성화효소; (v) 포스포글루코무타아제 또는 포도당 인산화 효소; 및/또는 (vi) α-글루칸 포스포릴라아제, 전분 포스포릴라아제, 말토덱스트린 포스포릴라아제, 수크로오스 포스포릴라아제, α-아밀라아제, 풀루란아제, 이소아밀라아제, 글루코아밀라아제, 알파글루카노트랜스퍼라아제, 폴리포스페이트 글루코카이네이즈또는 수크라아제]; 상기 싸이코스 제조 경로에 관여하는 효소를 발현하는 미생물; 또는 상기 싸이코스 제조 경로에 관여하는 효소를 발현하는 미생물의 배양물을 추가적으로 포함할 수 있다. 다만, 이는 예시적인 것으로 본 출원의 싸이코스-6-인산 탈인산화 효소를 이용하여 싸이코스를 생산할 수 있다면, 본 출원의 싸이코스 생산용 조성물에 포함되는 효소 및 싸이코스 생산에 이용되는 기질이 제한되지 않는다.
구체적으로, 상기 과당-6-인산-3-에피머화 효소는 과당-6-인산을 싸이코스-6-인산으로 전환하는 활성을 갖는 단백질이라면 어떠한 단백질도 포함할 수 있다. 상기 포도당-6-인산-이성화효소는 포도당-6-인산을 과당-6-인산으로 전환하는 활성을 갖는 단백질이라면 어떠한 단백질도 포함할 수 있다. 상기 포스포글루코무타아제(phosphoglucomutase, EC 5.4.2.2)는 포도당-1-인산을 포도당-6-인산으로 전환시키는 활성을 갖는 단백질이라면 어떠한 단백질도 포함할 수 있다. 상기 전분/말토덱스트린 포스포릴라아제(starch/maltodextrin phosphorylase, EC 2.4.1.1) 및 α-글루칸 포스포릴라아제는 포스페이트(phosphate)를 포도당에 인산화 전이시켜 전분 또는 말토덱스트린으로부터 포도당-1-인산을 생산하는 활성을 갖는 단백질이라면 어떠한 단백질도 포함 할 수 있다. 상기 수크로스 포스포릴라아제(sucrose phosphorylase, EC 2.4.1.7)는 포스페이트를 포도당에 인산화 전이시켜 수크로스로부터 포도당-1-인산을 생산하는 활성을 갖는 단백질이라면 어떠한 단백질도 포함할 수 있다. 상기 전분 액당화 효소인 α-아밀라아제(α-amylase, EC 3.2.1.1), 풀루란아제(pullulanse, EC 3.2.1.41), 이소아밀라아제(isoamylase, EC 3.2.1.68), 4-α-글루카노트렌스퍼레아제 (4-α-glucanotransferase, EC 2.4.1.25) 및 글루코아밀라아제(glucoamylase, EC 3.2.1.3) 는 전분 또는 말토덱스트린을 탈분지화된 말토올리고당 또는 포도당으로 전환시키는 활성을 갖는 단백질이라면 어떠한 단백질도 포함할 수 있다. 상기 수크라제(sucrase, EC 3.2.1.26)는 수크로스를 포도당으로 전환시키는 활성을 갖는 단백질이라면 어떠한 단백질도 포함할 수 있다. 폴리포스페이트 포도당인산화효소(polyphosphate glucokinase, EC 2.7.1.63)는 폴리포스페이트의 인산을 포도당에 전이시켜 포도당-6-인산으로 전환하는 활성을 가지는 단백질이라면 어떠한 단백질도 포함할 수 있다.
본 출원의 싸이코스 생산용 조성물에 포함되는 싸이코스-6-인산 탈인산효소, α-글루칸 포스포릴라아제, 포스포글루코뮤타아제(또는 포스포만노뮤타아제), 포도당-6-인산 이성화 효소, 싸이코스-6-인산-3-에피머화효소(또는 리불로스-5-인산-3-에피머화효소), 풀루라네이즈(또는 아이소아밀레이즈), 4-α-글루카노트렌스퍼레아제, 폴리포스페이트 포도당인산화효소 등은 최종 산물인 싸이코스에 대한 부반응이 없거나 적은 것일 수 있다.
본 출원의 싸이코스 생산용 조성물은 싸이코스-6-인산의 탈인산화 효소 뿐만 아니라, 싸이코스를 제조하기 위한 복수 개의 효소 및 이의 기질을 적절히 포함할 수 있고, 여러 효소가 존재하는 환경에서도 본 출원의 싸이코스-6-인산의 탈인산화 효소는 선택적이고 비가역적으로 싸이코스-6-인산으로부터 싸이코스를 제조할 수 있는 효과를 가진다.
본 출원의 싸이코스 생산용 조성물은 당해 싸이코스 생산용 조성물에 통상 사용되는 임의의 적합한 부형제를 추가로 포함할 수 있다. 이러한 부형제에는, 예를 들어, 보존제, 습윤제, 분산제, 현탁화제, 완충제, 안정화제 및 등장화제 등이 포함될 수 있으나, 이에 한정되는 것은 아니다.
본 출원의 싸이코스 생산용 조성물은 금속 이온 또는 금속염을 추가로 포함할 수 있다. 일 구현예에서, 상기 금속 이온은 2가 양이온일 수 있으며, 구체적으로 Ni, Mg, Ni, Co, Mn, Fe 및 Zn으로 이루어진 군으로부터 선택되는 1종 이상의 금속 이온일 수 있다. 보다 구체적으로, 본 출원의 싸이코스 생산용 조성물은 금속염을 추가로 포함할 수 있으며, 보다 더 구체적으로 상기 금속염은 NiSO4, MgSO4, MgCl2, NiCl2, CoSO4, CoCl2, MnCl2, MnSO4, FeSO4 및 ZnSO4로 이루어진 군에서 선택되는 1종 이상일 수 있다.
본 출원의 또 다른 하나의 양태는 싸이코스-6-인산 탈인산화 효소, 이를 발현하는 미생물, 또는 상기 미생물의 배양물을 싸이코스-6-인산과 접촉시켜 싸이코스-6-인산을 싸이코스로 전환하는 단계를 포함하는, 싸이코스 제조방법을 제공하는 것이다.
구체적으로, 본 출원의 싸이코스 제조방법은 싸이코스-6-인산 탈인산화 효소, 이를 발현하는 미생물, 또는 상기 미생물의 배양물을 싸이코스-6-인산에 접촉하여 싸이코스를 제조하는 것일 수 있으나, 이에 제한되지 않는다.
본 출원의 방법은 싸이코스-6-인산을 싸이코스로 전환하는 단계 이전, 과당-6-인산(fructose-6-phosphate)에 과당-6-인산-3-에피머화 효소, 상기 과당-6-인산-3-에피머화 효소를 발현하는 미생물 또는 상기 과당-6-인산-3-에피머화 효소를 발현하는 미생물의 배양물을 접촉시켜, 상기 과당-6-인산을 싸이코스-6-인산으로 전환하는 단계를 추가적으로 포함할 수 있다.
본 출원의 방법은 상기 과당-6-인산을 싸이코스-6-인산으로 전환하는 단계 이전, 포도당-6-인산(Glucose-6-phosphate)에 포도당-6-인산-이성화효소, 상기 포도당-6-인산-이성화효소를 발현하는 미생물 또는 상기 포도당-6-인산-이성화효소를 발현하는 미생물의 배양물을 접촉시켜, 상기 포도당-6-인산을 과당-6-인산으로 전환하는 단계를 추가적으로 포함할 수 있다.
본 출원의 방법은 상기 포도당-6-인산을 과당-6-인산으로 전환하는 단계 이전, 포도당-1-인산(Glucose-1-phosphate)에 포스포글루코무타아제, 상기 포스포글루코무타아제를 발현하는 미생물 또는 상기 포스포글루코무타아제를 발현하는 미생물의 배양물을 접촉시켜, 상기 포도당-1-인산을 포도당-6-인산으로 전환하는 단계를 추가적으로 포함할 수 있다.
본 출원의 방법은 상기 포도당-6-인산을 과당-6-인산으로 전환하는 단계 이전, 포도당(Glucose)에 폴리포스페이트 포도당인산화 효소, 상기 폴리포스페이트 포도당인산화 효소를 발현하는 미생물 또는 상기 폴리포스페이트 포도당 인산화 효소를 발현하는 미생물의 배양물, 및 폴리포스페이트를 접촉시켜, 상기 포도당을 포도당-6-인산으로 전환하는 단계를 추가적으로 포함할 수 있다.
본 출원의 방법은 상기 포도당-1-인산을 포도당-6-인산으로 전환하는 단계 이전, 전분, 말토덱스트린, 수크로스 또는 이의 조합에 α-글루칸 포스포릴라아제, 전분 포스포릴라아제, 말토덱스트린 포스포릴라아제 또는 수크로오스 포스포릴라아제; 상기 포스포릴라아제를 발현하는 미생물; 또는 상기 포스포릴라아제를 발현하는 미생물의 배양물, 및 포스페이트를 접촉시켜, 상기 전분, 말토덱스트린, 수크로스 또는 이의 조합을 포도당-1-인산으로 전환하는 단계를 추가적으로 포함할 수 있다.
본 출원의 방법은 상기 전분, 말토덱스트린, 수크로스 또는 이의 조합을 포도당-1-인산 으로 전환하는 단계 이전, 전분, 말토덱스트린, 수크로스 또는 이의 조합에 α-아밀라아제, 풀루란아제, 이소아밀라아제, 글루코아밀라아제 또는 수크라아제; 상기 α-아밀라아제, 풀루란아제, 글루코아밀라아제, 수크라아제 또는 이소아밀라아제를 발현하는 미생물; 또는 상기 α-아밀라아제, 풀루란아제, 글루코아밀라아제, 수크라아제 또는 이소아밀라아제를 발현하는 미생물의 배양물을 접촉시켜, 상기 전분, 말토덱스트린, 수크로스 또는 이의 조합을 포도당으로 전환하는 단계를 추가적으로 포함할 수 있다.
본 출원의 싸이코스 제조 방법에 이용되는 싸이코스-6-인산 탈인산효소, α-글루칸 포스포릴라아제, 포스포글루코뮤타아제(또는 포스포만노뮤타아제), 포도당-6-인산 이성화 효소, 싸이코스-6-인산-3-에피머화효소(또는 리불로스-5-인산-3-에피머화효소), 풀루라네이즈(또는 아이소아밀레이즈), 4-α-글루카노트렌스퍼레아제, 폴리포스페이트 포도당인산화효소 등은 최종 산물인 싸이코스에 대한 부반응이 없거나 적은 것일 수 있다.
본 출원의 싸이코스 제조 방법은 고농도 전분을 분해하여 인산당 전환 효소들과 복합적 조합으로 최적/최대의 싸이코스를 생산하는 것일 수 있고, 싸이코스의 최대 생산성 확보를 위하여 최대 8종류의 효소를 조합하여 사용할 수 있다.
첫번째로, 전분을 분해하며 포도당-1-인산을 생산하는 효소인 글루칸 포스포릴라아제(glucan phosphorlase, glycogen phosphorylase, EC 2.4.1.1)는 α-1,4 결합된 전분에 특이적으로 포도당-1-인산(glucose-1-phosphate)을 생성한다. 둘째로는 이렇게 생성된 포도당-1-인산을 포도당-6-인산(glucose-6-phosphate)으로 전환하는 포스포글루코뮤타아제(phosphoglucomutase, EC 2.7.5.1) 또는 포스포만노뮤타아제(phosphomannomutase, EC 5.4.2.8)가 중간 복합 효소 반응에 쓰이게 된다. 세번째 사용 효소로는 포도당-6인산을 과당-6-인산(fructose-6-phosphate)으로 전환하는 포도당-6-인산 이성화 효소(glucose-6-phosphate isomerase, EC 5.3.1.9)가 사용된다. 네번째로 앞서 설명된, 과당-6-인산에서 싸이코스-6-인산으로 전환하는 효소인 리불로스-5-인산-3-에피머화효소 또는 싸이코스-6-인산-3-에피머화효소를 활용하여 가역반응의 싸이코스-6-인산까지 생성 가능하다. 전분부터 싸이코스-6-인산 반응까지는 가역 반응으로 일정량 이상 생성이 불가능 하지만, 본 발명에 포함된 싸이코스-6-인산 선택적인 탈인산 효소(psicose-6-phosphate phosphatase) 사용시 높은 수율의 싸이코스 생산이 가능하다.
부가적으로 전분 이용율을 높이기 위하여 아밀로펙틴(amylopectin)의 α-1,4 외 α-1,6 결합의 가지(Branch) 결합을 분해하기 위하여 풀루라네이즈(pullulanase, EC 3.2.1.41) 또는 이소아밀레이즈(isoamylase EC 3.2.1.68) 효소가 같이 사용되며, 또한 글루칸 포스포릴라아제의 전분 활용을 높이기 위하여 글루카노트렌스퍼레아제(4-alpha-glucanotransferase, EC 2.4.1.25)를 활용한다. 상대적으로 활성이 낮은 기질인 맥아당(maltose) 또는 기타 올리고당에 α-1,4 결합형태로 올리고당을 결합하여 세분화된 전분기질의 이용율을 높일 수 있다. 추가적으로 폴리포스페이트 글루코카이네이즈(polyphosphate-glucose phosphotransferase, EC 2.7.1.63)을 사용하여 전분 사용 후 분해된 포도당에서 복합 효소반응을 통하여 추가적인 싸이코스 생산이 가능하여 최대 싸이코스 전환율을 확보 가능하다.
또한, 본 출원의 제조방법에 있어서, 본 출원의 접촉은 pH 5.0 내지 9.0, 구체적으로 pH 6.0 내지 8.0에서 실시할 수 있다.
본 출원의 제조방법에 있어서, 본 출원의 접촉은 40℃ 내지 80℃ 온도, 구체적으로 40℃ 내지 60℃ 또는 50℃ 내지 60℃ 온도 에서 실시할 수 있다.
본 출원의 제조방법에 있어서, 본 출원의 접촉은 2시간 내지 24 시간, 구체적으로 6 내지 24시간~120시간 동안 실시할 수 있다.
본 출원의 제조방법에 있어서, 본 출원의 접촉은 pH 5.0 내지 9.0, 40℃ 내지 80℃ 온도, 및/또는 2시간 내지 24 시간 동안 실시할 수 있다. 구체적으로, 상기 접촉은 pH 6.0 내지 8.0, 40℃ 내지 60℃ 또는 50℃ 내지 60℃ 온도, 및/또는 6시간 내지 24시간~120시간 동안 실시할 수 있다.
본 출원의 제조방법은 싸이코스를 정제하는 단계를 추가로 포함할 수 있다. 본 출원의 정제는 특별히 제한되지 아니하며, 본 출원의 기술 분야에서 통상적으로 사용하는 방법을 사용할 수 있다. 비제한적인 예로, 크로마토그래피, 분별 결정 및 이온 정제 등을 들 수 있다. 상기 정제 방법은 하나만 실시될 수도 있으며, 두 가지 이상의 방법을 함께 실시할 수도 있다. 예를 들어, 크로마토그래피를 통해 싸이코스 생성 반응물을 정제할 수 있으며, 상기 크로마토그래피에 의한 당의 분리는 분리하고자 하는 당과 이온 수지에 부착된 금속 이온 사이의 약한 결합력의 차이를 이용하여 수행될 수 있다.
또한, 본 출원은 본 출원의 정제하는 단계의 전 또는 후에 탈색, 탈염 또는 둘 다를 실시하는 것을 추가로 포함할 수 있다. 상기 탈색 및/또는 탈염을 실시함으로써, 불순물 없이 보다 정제된 싸이코스 반응물을 얻을 수 있다.
본 출원의 신규한 각 효소 및 그 조합은 내열성이 있어 싸이코스-6-인산을 싸이코스로 전환하는 경로를 산업적으로 수행할 수 있고, 경제적인 원료인 포도당 또는 전분(예컨대, 말토덱스트린)을 사용하여 싸이코스 합성 경로의 진행을 가능하게 하며, 비가역 반응 경로인 싸이코스-6-인산 탈인산화 반응에 의하여 싸이코스 생산을 가능하게 하는바 싸이코스로의 전환율을 현저히 높일 수 있다. 내열성 효소는 상업적으로 미생물 방지와 고농도 기질 반응이 용이하여 그 효과가 더욱 부가 된다. 또한, 본 제조방법은 싸이코스 전환율 상승에 의하여 반응 결과물이 고농도의 싸이코스를 포함하여 분리정제 공정을 단순화 또는 제거할 수 있는바, 제조방법이 간단하면서도 경제적인 이점이 있다. 특히 SMB를 사용한 분리 등을 안하거나 최소화 가능하여 분리 효율 및 수율을 극대화 할 수 있다.
이하, 본 출원을 실시예를 들어 상세히 설명하고자 하나, 이는 본 출원의 이해를 돕기 위한 것일 뿐, 본 출원의 범위가 이에 한정되는 것은 아니다.
본 출원에서 아미노산은 아래와 같은 약어 또는 아미노산 명으로 표시될 수 있다:
아미노산 종류 약어 해당 아미노산으로
코딩 되는 DNA codon 		해당 아미노산으로
코딩 되는 RNA codon
알라닌(alanine) A GCT, GCC, GCA, GCG GCU, GCC, GCA, GCG
아르기닌(arginine) R AGA, AGG AGA, AGG
아스파라긴(asparagines) N AAT, AAC AAU, AAC
아스파르트산(aspartic acid) D GAT, GAC GAU, GAC
시스테인(cystein) C TGT, TGC UGU, UGC
글루탐산(glutamic acid) E GAA, GAG GAA, GAG
글루타민(glutamine) Q CAA, CAG CAA, CAG
글라이신(glycine) G GGT, GGC, GGA, GGG GGU, GGC, GGA, GGG
히스티딘(histidine) H CAC, CAT CAC, CAU
이소류신(isoleucine) I ATT, ATC, ATA AUU, AUC, AUA
류신(leucine) L TTA, TTG, CTT, CTC, CTA, CTG UUA, UUG, CUU, CUC, CUA, CUG
라이신(lycine) K AAA, AAG AAA, AAG
메티오닌(methionine) M ATG AUG
페닐알라닌(phenylalanine) F TTT, TTC UUU, UUC
프롤린(proline) P CCT, CCC, CCA, CCG CCU, CCC, CCA, CCG
세린(serine) S TCT, TCC, TCA, TCG UCU, UCC, UCA, UCG
트레오닌(threonine) T ACT, ACC, ACA, ACG ACU, ACC, ACA, ACG
트립토판(tryptophan) W TGG UGG
타이로신(tyrosine) Y TAT, TAC UAU, UAC
발린(valine) V GTT, GTC, GTA, GTG GUU, GUC, GUA, GUG
실시예 1: 각 효소의 재조합 발현 벡터 및 형질전환 미생물의 제조
본 출원의 싸이코스 제조 경로에 필요한 각 효소를 제공하기 위하여 내열성의 유전자를 선발하였으며, 이를 정리하면 하기 표 2와 같다.
서열번호 종 속 NCBI No.
1 Alicyclobacillus acidocaldarius ACV59297.1
2 Alicyclobacillus tengchongensis KRW92667.1
3 WP_058093972.1
4 Amycolatopsis thermoflava WP_017986193.1
5 WP_037323890.1
6 Anaerolinea thermolimosa WP_084001059.1
7 WP_062189018.1
8 GAP08215.1
9 Anaerolinea thermophila BAJ62794.1
10 KUK46216.1
11 BAJ63987.1
12 Archaeoglobus fugidus AAB91288.1
13 AAB89113.1
14 AAB89416.1
15 Archaeoglobus profundus WP_012940213.1
16 Archaeoglobus veneficus WP_013683862.1
17 Bacillus licheniformis AAU41557.1
18 AAU39603.1
19 AAU43073.1
20 Caldicellulosiruptor bescii ACM59531.1
21 ACM59457.1
22 ACM61198.1
23 ACM61211.1
24 ACM60182.1
25 Caldilinea aerophila BAL98676.1
26 BAM00874.1
27 WP_014433277.1
28 Caldithrix abyssi APF18124.1
29 APF18744.1
30 APF16831.1
31 WP_006929206.1
32 Carboxydocella sp. ULO1 WP_079933669.1
33 Carboxydothermus ferrireducens WP_028051691.1
34 WP_028051711.1
35 WP_028053062.1
36 Chloroflexi bacterium 54-19 OJW02856.1
37 Defluviitoga tunisiensis WP_045087739.1
38 Deinococcus aerius WP_103127908.1
39 Deinococcus apachensis WP_019584763.1
40 Deinococcus aquatilis WP_019011981.1
41 Deinococcus geothermalis WP_011529832.1
42 Deinococcus hopiensis WP_084049191.1
43 Deinococcus maricopensis WP_013556873.1
44 Deinococcus murrayi WP_084542862.1
45 WP_051363537.1
46 Deinococcus reticulitermitis WP_092263046.1
47 Deinococcus wulumuqiensis WP_017870657.1
48 Deinococcus sp. Leaf326 WP_056297006.1
49 Deinococcus phoenicis WP_034353069.1
50 Deinococcus proteolyticus WP_013614672.1
51 Deinococcus sp. 17bor-2 WP_109826642.1
52 Deinococcus sp. NW-56 WP_104990527.1
53 Deinococcus sp. RL KEF35131.1
54 WP_081851636.1
55 Deinococcus sp. YIM 77859 WP_034384462.1
56 Desulfurococcus mucosus ADV64381.1
57 ADV64382.1
58 ADV64566.1
59 Dictyoglomus turgidum YP_002352793.1
60 YP_002353123.1
61 YP_002352154.1
62 Effusibacillus pohliae WP_018132070.1
63 Fervidobacterium gondwanense WP_072759218.1
64 Fervidobacterium islandicum AMW32328.1
65 AMW33413.1
66 Fervidobacterium nodosum ABS60322.1
67 ABS61294.1
68 Fervidobacterium pennivorans AFG34634.1
69 AFG35917.1
70 WP_064011782.1
71 Geobacillus sp. WP_011232549.1
72 Geobacillus stearothermophilus ALA69320.1
73 ALA71677.1
74 ALA71036.1
75 ALA70927.1
76 ALA69332.1
77 Halococcus salifodinae WP_005045395.1
78 EMA49396.1
79 EMA52756.1
80 Hydrogenivirga sp. 128-5-R1-1 WP_008285887.1
81 Hydrogenobacter hydrogenophilus WP_096602199.1
82 WP_096601840.1
83 Hydrogenobacter thermophilus WP_012963417.1
84 Hyperthermus butylicus ABM80367.1
85 Kosmotoga arenicorallina WP_084251417.1
86 Kosmotoga olearia ACR80305.1
87 ACR80819.1
88 Marinitoga piezophila AEX84499.1
89 AEX85453.1
90 AEX85799.1
91 Meiothermus cerbereus WP_027877060.1
92 WP_027878515.1
93 Meiothermus chliarophilus WP_027892542.1
94 WP_051304156.1
95 Meiothermus ruber ADD26908.1
96 WP_013013826.1
97 WP_013013825.1
98 WP_013012548.1
99 WP_027883570.1
100 Meiothermus silvanus ADH63424.1
101 WP_013157023.1
102 Meiothermus taiwanensis WP_027888084.1
103 WP_027888305.1
104 Meiothermus timidus WP_018466213.1
105 WP_018465080.1
106 WP_018467688.1
107 WP_018467518.1
108 WP_018465603.1
109 WP_018466116.1
110 WP_018467797.1
111 WP_018467420.1
112 WP_018466214.1
113 WP_018465420.1
114 Meiothermus rufus WP_036271046.1
115 Mesotoga infera KUK90417.1
116 Metallosphaera sedula ABP94905.1
117 Methanocella conradii AFC99407.1
118 WP_014405429.1
119 AFD00405.1
120 Methanococcoides methylutens WP_048194361.1
121 AKB84306.1
122 Methanohalobium evestigatum ADI75005.1
123 WP_013193898.1
124 Methanolobus tindarius WP_048135864.1
125 WP_048135649.1
126 ETA67773.1
127 Methanosarcina siciliae WP_048179383.1
128 AKB37722.1
129 AKB35909.1
130 Methanothermus fervidus ADP76907.1
131 Petrotoga mobilis ABX30802.1
132 KUK15285.1
133 Picrophilus torridus AAT44010.1
134 AAT43693.1
135 Pseudonocardia thermophila WP_073456926.1
136 Pyrococcus furiosus AAL80556.1
137 AAL81109.1
138 WP_014835302.1
139 AAL82045.1
140 AAL82122.1
141 AAL81285.1
142 AAL81790.1
143 AAL80346.1
144 AAL80703.1
145 AAL81901.1
146 AAL81913.1
147 AAL80587.1
148 AFN04628.1
149 Pyrodictium occultum WP_083494490.1
150 Rhodothermus marinus WP_041806336.1
151 WP_012844425.1
152 ACY47561.1
153 ACY48134.1
154 ACY47672.1
155 ACY48911.1
156 WP_012843092.1
157 WP_012843709.1
158 WP_012844300.1
159 ACY48106.1
160 WP_012844667.1
161 WP_012845116.1
162 WP_012844208.1
163 WP_012843855.1
164 ACY47289.1
165 Slackia heliotrinireducens WP_012798605.1
166 ACV22623.1
167 Staphylothermus marinus ABN70247.1
168 ABN69637.1
169 ABN69899.1
170 Sulfolobus acidocaldarius AAY79446.1
171 WP_015385352.1
172 AAY80292.1
173 Thermanaerothrix daxensis KPL83857.1
174 Thermoanaerobacter sp. WP_004400108.1
175 Thermoanaerobacter thermohydrosulfuricus WP_074592559.1
176 WP_074665888.1
177 Thermoanaerobacter wiegelii AEM79256.1
178 AEM79130.1
179 Thermoanaerobacterium xylanolyticum WP_013788008.1
180 Thermobifida halotolerans WP_068689397.1
181 WP_068692137.1
182 Thermococcus celer WP_088862065.1
183 ASI98087.1
184 Thermococcus litoralis EHR78031.1
185 Thermococcus profundus WP_088857759.1
186 Thermocrinis minervae WP_079653666.1
187 Thermocrinis ruber AHE95294.1
188 Thermoflexus hugenholtzii WP_088571736.1
189 Thermotoga lettingae ABV34293.1
190 Thermotoga neapolitana WP_015919708.1
191 ACM22182.1
192 ACM22192.1
193 ACM22252.1
194 ACM22284.1
195 ACM22382.1
196 ACM22666.1
197 ACM22704.1
198 ACM22708.1
199 ACM22832.1
200 ACM22990.1
201 ACM23016.1
202 ACM23040.1
203 ACM23081.1
204 ACM23138.1
205 ACM23254.1
206 ACM23323.1
207 ACM23341.1
208 ACM23415.1
209 ACM23510.1
210 ACM23571.1
211 ACM23575.1
212 ACM23847.1
213 ACM23859.1
214 ACM23950.1
215 ACM23957.1
216 ACM24018.1
217 Thermotoga petrophilia ABQ46309.1
218 Thermus amyloliquefaciens WP_038055695.1
219 Thermus filiformis WP_038062097.1
220 Thermus thermophilus WP_096411270.1
221 AAS82270.1
222 Truepera radiovictrix WP_013176662.1
상기 선발한 아미노산의 유전자는 유전자 합성 또는 각 분양 균주의 염색체 DNA(genomic DNA)로부터 각각의 유전자를 중합효소 연쇄반응(PCR)을 이용하여 증폭하고, 증폭된 각각의DNA는 제한효소 NdeⅠ 및 XhoⅠ 또는 Sal I을 사용하여 대장균 발현용 플라스미드 벡터 pET21a(Novagen사)에 삽입하여 재조합 발현벡터를 제작 하였다. 상기 발현벡터는 통상적인 형질전환 방법[참조: Sambrook et al. 1989]으로 대장균(E. coli) BL21(DE3) 균주에 각각 형질 전환하여 형질전환 미생물을 제조하였다.
구체적으로, 서열번호 26, 29, 53, 56, 60, 70, 76, 80, 81, 116, 117, 131, 134, 145, 167, 185, 186, 및 191의 싸이코스-6-인산 탈인산 효소를 대장균(E. coli) BL21(DE3) 균주에 각각 형질 전환하여 E.coil BL21(DE3)/pET-CJ-ap26, E.coil BL21(DE3)/pET-CJ-ap29, E.coil BL21(DE3)/pET-CJ-ap53, E.coil BL21(DE3)/pET-CJ-ap56, E.coil BL21(DE3)/pET-CJ-ap60, E.coil BL21(DE3)/pET-CJ-ap70, E.coil BL21(DE3)/pET-CJ-ap76, E.coil BL21(DE3)/pET-CJ-ap80, E.coil BL21(DE3)/pET-CJ-ap81, E.coil BL21(DE3)/pET-CJ-ap116, E.coil BL21(DE3)/pET-CJ-ap117, E.coil BL21(DE3)/pET-CJ-ap131, E.coil BL21(DE3)/pET-CJ-ap134, E.coil BL21(DE3)/pET-CJ-ap145, E.coil BL21(DE3)/pET-CJ-ap167, E.coil BL21(DE3)/pET-CJ-ap185, E.coil BL21(DE3)/pET-CJ-ap186, 및 E.coil BL21(DE3)/pET-CJ-ap191 로 명명된 형질전환 미생물을 제조하였다.
상기 제조된 형질전환 미생물들을 국제기탁기관인 한국미생물보존센터(KCCM)에 2018년 11월 14일자로 기탁하여 KCCM12390P(E.coil BL21(DE3)/pET-CJ-ap26), KCCM12391P(E.coil BL21(DE3)/pET-CJ-ap29), KCCM12392P(E.coil BL21(DE3)/pET-CJ-ap53), KCCM12393P(E.coil BL21(DE3)/pET-CJ-ap56), KCCM12394P(E.coil BL21(DE3)/pET-CJ-ap60), KCCM12395P(E.coil BL21(DE3)/pET-CJ-ap70), KCCM12396P(E.coil BL21(DE3)/pET-CJ-ap76), KCCM12397P(E.coil BL21(DE3)/pET-CJ-ap80), KCCM12398P(E.coil BL21(DE3)/pET-CJ-ap81), KCCM12399P(E.coil BL21(DE3)/pET-CJ-ap116), KCCM12400P(E.coil BL21(DE3)/pET-CJ-ap117), KCCM12401P(E.coil BL21(DE3)/pET-CJ-ap131), KCCM12402P(E.coil BL21(DE3)/pET-CJ-ap134), KCCM12403P(E.coil BL21(DE3)/pET-CJ-ap145), KCCM12404P(E.coil BL21(DE3)/pET-CJ-ap167), KCCM12405P(E.coil BL21(DE3)/pET-CJ-ap185), KCCM12406P(E.coil BL21(DE3)/pET-CJ-ap186), KCCM12407P(E.coil BL21(DE3)/pET-CJ-ap191)의 기탁 번호를 각각 부여 받았다.
실시예 2: 재조합 효소의 제조
재조합 효소를 제조하기 위해, 실시예 1에서 제조한 각 형질전환 미생물을 LB 액체배지 5 ml를 포함하는 배양 튜브에 접종하고, 600 nm에서 흡광도가 2.0이 될 때까지 37 ℃의 진탕 배양기에서 종균 배양을 하였다. 본 종균 배양된 배양액을 LB 액체배지를 포함하는 배양 플라스크에 접종하여 본 배양을 진행하였다. 600 nm에서의 흡광도가 2.0이 될 때 1 mM IPTG를 첨가하여 재조합 효소의 발현생산을 유도하였다. 상기 배양 과정 중의 교반 속도는 180 rpm이며 배양 온도는 37 ℃가 유지되도록 하였다. 배양액은 8,000×g로 4 ℃에서 20분 동안 원심분리 후 균체를 회수하였다. 회수된 균체는 50 mM Tris-HCl(pH 8.0) 완충용액으로 2회 세척하였고, 동일 완충용액으로 현탁 후 초음파 세포파쇄기를 이용하여 세포를 파쇄하였다. 세포 파쇄물은 13,000×g로 4 ℃에서 20분 동안 원심분리 후 상등액만을 취하였다. 상기 상등액으로부터 His-tag 친화 크로마토그래피를 사용하여 재조합 효소를 정제하고, 50 mM Tris-HCl(pH 8.0) 완충용액으로 투석한 후 반응에 사용하였다.
실시예 3: 인산당 탈인산 활성 및 싸이코스-6-인산 탈인산 활성 분석
과당-6-인산으로부터 싸이코스-6-인산을 제조한 후 싸이코스-6-인산 탈인산 효소 의한 싸이코스 생산 활성을 확인 또는 포도당-1-인산을 최초 기질로 3개의 효소를 추가적으로 넣어 싸이코스를 검출하여 활성 측정 하였다. 여기서 효소 3개는 포도당-1-인산을 포도당-6-인산(glucose-6-phosphate)으로 전환하는 포스포글루코뮤타아제(phosphoglucomutase, EC 2.7.5.1) 또는 포스포만노뮤타아제(phosphomannomutase, EC 5.4.2.8)와 포도당-6인산을 과당-6-인산(fructose-6-phosphate)으로 전환하는 포도당-6-인산 이성화 효소(glucose-6-phosphate isomerase, EC 5.3.1.9) 또한 과당-6-인산에서 싸이코스-6-인산으로 전환하는 효소인 리불로스-5-인산-3-에피머화효소 또는 싸이코스-6-인산-3-에피머화효소를 본 출원의 탈인산 효소 222종 각각과 혼합하여 생성되는 일반당인 포도당, 과당, 싸이코스를 정성 및 정량 평가하여 확인 하였다.
구체적으로, 50 mM 과당-6-인산 또는 20 mM 포도당-1-인산을 50 mM Tris-HCl(pH 7.0) 또는 50 mM Sodium-posphate(pH 6~7) 또는 50 mM Potassium-posphate(pH 6~7) 완충용액에 현탁한 후, 포스포글루코뮤타아제 또는 포스포만노뮤타아제와 포도당-6-인산 이성화 효소와 리불로스-5-인산-3-에피머화효소 또는 싸이코스-6-인산-3-에피머화효소 및 실시예 2에서 제조한 재조합 싸이코스-6-인산 탈인산 효소 222종을 각각 0.1 unit/ml 첨가하고, 45℃~70℃에서 1~24 시간 동안 반응시켰다. HPLC를 이용하여 포도당, 과당, 싸이코스 생성 여부를 분석하였으며, HPLC 분석은 SP_0810(Shodex사) 컬럼과 Aminex HPX-87C(Bio-RAD사) 컬럼을 사용하여 80℃에서 이동상으로 0.6 ml/min 유속으로 흘려 주면서 수행하였으며, Refractive Index Detector(RID)로 검출하였다.
이후, 제조된 포도당-1-인산, 포도당-6-인산, 과당-6-인산 및 싸이코스-6-인산의 혼합액에서 본 출원의 싸이코스-6-인산의 탈인산화 효소의 탈인산 활성 및 싸이코스-6-인산 특이적(선택적) 탈인산화율을 측정하였다.
구체적으로, 0.1 unit/ml의 각각의 본 출원의 싸이코스-6-인산의 탈인산화 효소 및 5 mM MgCl2(또는 MgSO4)을 1 %(w/v) 포도당-6-인산, 포도당-1-인산, 과당-6-인산 및 싸이코스-6-인산의 혼합액에 첨가하여 50℃에서 12시간 반응시킨 후, HPLC를 이용하여 반응물을 분석하였다. HPLC 분석은 Aminex HPX-87C(Bio-RAD사) 컬럼을 사용하여 80℃에서 이동상으로 0.6 ml/min 유속으로 흘려 주면서 수행하였고 Refractive Index Detector로 생성된 싸이코스 및 기타당(과당 및 포도당)을 검출하였다.
그 결과, 모든 222종 효소중 69개의 효소에서 싸이코스-6-인산에 대해 높은 선택적 탈인산 활성을 확인하였으며, 45개 효소에서 싸이코스-6-인산의 약한 선택적 탈인산 활성을 확인 하였다. 108개 효소에서는 선택적 싸이코스-6-인산 탈인산 역가는 확인 할 수 없었다. 다만, 222종 중 1개를 제외한 221종의 효소에서 탈인산 활성을 확인하였으며, 이중 113개 효소는 높은 탈인산 활성을 확인하였으며, 108종의 효소에서 낮은 탈인산 활성을 확인 하였다. 한편, 탈인산 역가가 없는 것으로 확인된 서열번호 54의 효소는 탈인산 효소로서 알려져 있으나, 실제로는 탈인산 활성이 없는 것으로 확인되었다. 해당 결과는 하기 표 3과 같이 정리 하였다.
A = 낮은 활성 (인산당에서 단당으로의 탈인산 전환율이 1% ~ 30%)
B = 높은 활성 (인산당에서 단당으로의 탈인산 전환율이 30% 이상)
C = 없는 활성 (인산당에서 단당으로의 탈인산 전환율이 1% 미만)
서열번호 종 속 NCBI No. G1P/G6P/F6P/P6P
탈인산 활성		 P6P(선택적)
/G1P+G6P+F6P+P6P
탈인산 활성
1 Alicyclobacillus acidocaldarius ACV59297.1 B B
2 Alicyclobacillus tengchongensis KRW92667.1 A C
3 WP_058093972.1 A C
4 Amycolatopsis thermoflava WP_017986193.1 A C
5 WP_037323890.1 A C
6 Anaerolinea thermolimosa WP_084001059.1 B B
7 WP_062189018.1 A C
8 GAP08215.1 A C
9 Anaerolinea thermophila BAJ62794.1 B B
10 KUK46216.1 A C
11 BAJ63987.1 A C
12 Archaeoglobus fugidus AAB91288.1 B B
13 AAB89113.1 B A
14 AAB89416.1 B A
15 Archaeoglobus profundus WP_012940213.1 A C
16 Archaeoglobus veneficus WP_013683862.1 A C
17 Bacillus licheniformis AAU41557.1 B A
18 AAU39603.1 B A
19 AAU43073.1 A C
20 Caldicellulosiruptor bescii ACM59531.1 B A
21 ACM59457.1 B A
22 ACM61198.1 B A
23 ACM61211.1 B A
24 ACM60182.1 A C
25 Caldilinea aerophila BAL98676.1 B A
26 BAM00874.1 B B
27 WP_014433277.1 A C
28 Caldithrix abyssi APF18124.1 B A
29 APF18744.1 B B
30 APF16831.1 A C
31 WP_006929206.1 A C
32 Carboxydocella sp. ULO1 WP_079933669.1 A C
33 Carboxydothermus ferrireducens WP_028051691.1 A C
34 WP_028051711.1 A C
35 WP_028053062.1 A C
36 Chloroflexi bacterium 54-19 OJW02856.1 A C
37 Defluviitoga tunisiensis WP_045087739.1 B A
38 Deinococcus aerius WP_103127908.1 B B
39 Deinococcus apachensis WP_019584763.1 B B
40 Deinococcus aquatilis WP_019011981.1 B B
41 Deinococcus geothermalis WP_011529832.1 B B
42 Deinococcus hopiensis WP_084049191.1 B B
43 Deinococcus maricopensis WP_013556873.1 B B
44 Deinococcus murrayi WP_084542862.1 B A
45 WP_051363537.1 B B
46 Deinococcus reticulitermitis WP_092263046.1 B B
47 Deinococcus wulumuqiensis WP_017870657.1 B B
48 Deinococcus sp. Leaf326 WP_056297006.1 B B
49 Deinococcus phoenicis WP_034353069.1 B B
50 Deinococcus proteolyticus WP_013614672.1 B B
51 Deinococcus sp. 17bor-2 WP_109826642.1 B B
52 Deinococcus sp. NW-56 WP_104990527.1 B B
53 Deinococcus sp. RL KEF35131.1 B B
54 WP_081851636.1 C C
55 Deinococcus sp. YIM 77859 WP_034384462.1 B A
56 Desulfurococcus mucosus ADV64381.1 B B
57 ADV64382.1 B B
58 ADV64566.1 A C
59 Dictyoglomus turgidum YP_002352793.1 B B
60 YP_002353123.1 B B
61 YP_002352154.1 B A
62 Effusibacillus pohliae WP_018132070.1 A C
63 Fervidobacterium gondwanense WP_072759218.1 A C
64 Fervidobacterium islandicum AMW32328.1 B B
65 AMW33413.1 B B
66 Fervidobacterium nodosum ABS60322.1 B B
67 ABS61294.1 A C
68 Fervidobacterium pennivorans AFG34634.1 A C
69 AFG35917.1 B B
70 WP_064011782.1 B B
71 Geobacillus sp. WP_011232549.1 A C
72 Geobacillus stearothermophilus ALA69320.1 B B
73 ALA71677.1 B A
74 ALA71036.1 A C
75 ALA70927.1 A C
76 ALA69332.1 B B
77 Halococcus salifodinae WP_005045395.1 B A
78 EMA49396.1 A C
79 EMA52756.1 A C
80 Hydrogenivirga sp. 128-5-R1-1 WP_008285887.1 B B
81 Hydrogenobacter hydrogenophilus WP_096602199.1 B B
82 WP_096601840.1 A C
83 Hydrogenobacter thermophilus WP_012963417.1 A C
84 Hyperthermus butylicus ABM80367.1 A C
85 Kosmotoga arenicorallina WP_084251417.1 B A
86 Kosmotoga olearia ACR80305.1 A C
87 ACR80819.1 A C
88 Marinitoga piezophila AEX84499.1 B A
89 AEX85453.1 A C
90 AEX85799.1 A C
91 Meiothermus cerbereus WP_027877060.1 B B
92 WP_027878515.1 B B
93 Meiothermus chliarophilus WP_027892542.1 B B
94 WP_051304156.1 A C
95 Meiothermus ruber ADD26908.1 B B
96 WP_013013826.1 A C
97 WP_013013825.1 A C
98 WP_013012548.1 A C
99 WP_027883570.1 B B
100 Meiothermus silvanus ADH63424.1 B B
101 WP_013157023.1 B B
102 Meiothermus taiwanensis WP_027888084.1 B B
103 WP_027888305.1 B B
104 Meiothermus timidus WP_018466213.1 B A
105 WP_018465080.1 B A
106 WP_018467688.1 A C
107 WP_018467518.1 A C
108 WP_018465603.1 B A
109 WP_018466116.1 A C
110 WP_018467797.1 A C
111 WP_018467420.1 A C
112 WP_018466214.1 A C
113 WP_018465420.1 B B
114 Meiothermus rufus WP_036271046.1 B B
115 Mesotoga infera KUK90417.1 A C
116 Metallosphaera sedula ABP94905.1 B B
117 Methanocella conradii AFC99407.1 B B
118 WP_014405429.1 A C
119 AFD00405.1 A C
120 Methanococcoides methylutens WP_048194361.1 A C
121 AKB84306.1 A C
122 Methanohalobium evestigatum ADI75005.1 B A
123 WP_013193898.1 A C
124 Methanolobus tindarius WP_048135864.1 A C
125 WP_048135649.1 A C
126 ETA67773.1 A C
127 Methanosarcina siciliae WP_048179383.1 A C
128 AKB37722.1 A C
129 AKB35909.1 A C
130 Methanothermus fervidus ADP76907.1 A C
131 Petrotoga mobilis ABX30802.1 B B
132 KUK15285.1 A C
133 Picrophilus torridus AAT44010.1 B A
134 AAT43693.1 B B
135 Pseudonocardia thermophila WP_073456926.1 A C
136 Pyrococcus furiosus AAL80556.1 B B
137 AAL81109.1 A C
138 WP_014835302.1 A C
139 AAL82045.1 A C
140 AAL82122.1 A C
141 AAL81285.1 A C
142 AAL81790.1 B B
143 AAL80346.1 B A
144 AAL80703.1 B A
145 AAL81901.1 B B
146 AAL81913.1 B B
147 AAL80587.1 B A
148 AFN04628.1 B B
149 Pyrodictium occultum WP_083494490.1 A C
150 Rhodothermus marinus WP_041806336.1 B A
151 WP_012844425.1 B A
152 ACY47561.1 B A
153 ACY48134.1 A C
154 ACY47672.1 B A
155 ACY48911.1 B A
156 WP_012843092.1 B A
157 WP_012843709.1 A C
158 WP_012844300.1 A C
159 ACY48106.1 B A
160 WP_012844667.1 B A
161 WP_012845116.1 B A
162 WP_012844208.1 B A
163 WP_012843855.1 B A
164 ACY47289.1 B B
165 Slackia heliotrinireducens WP_012798605.1 A C
166 ACV22623.1 A C
167 Staphylothermus marinus ABN70247.1 B B
168 ABN69637.1 A C
169 ABN69899.1 B B
170 Sulfolobus acidocaldarius AAY79446.1 A C
171 WP_015385352.1 A C
172 AAY80292.1 B B
173 Thermanaerothrix daxensis KPL83857.1 B A
174 Thermoanaerobacter sp. WP_004400108.1 A C
175 Thermoanaerobacter thermohydrosulfuricus WP_074592559.1 B A
176 WP_074665888.1 A C
177 Thermoanaerobacter wiegelii AEM79256.1 B B
178 AEM79130.1 A C
179 Thermoanaerobacterium xylanolyticum WP_013788008.1 A C
180 Thermobifida halotolerans WP_068689397.1 A C
181 WP_068692137.1 A C
182 Thermococcus celer WP_088862065.1 A C
183 ASI98087.1 A C
184 Thermococcus litoralis EHR78031.1 B B
185 Thermococcus profundus WP_088857759.1 B B
186 Thermocrinis minervae WP_079653666.1 B B
187 Thermocrinis ruber AHE95294.1 B B
188 Thermoflexus hugenholtzii WP_088571736.1 A C
189 Thermotoga lettingae ABV34293.1 B B
190 Thermotoga neapolitana WP_015919708.1 A C
191 ACM22182.1 B B
192 ACM22192.1 B B
193 ACM22252.1 A C
194 ACM22284.1 A C
195 ACM22382.1 B A
196 ACM22666.1 A C
197 ACM22704.1 A C
198 ACM22708.1 B A
199 ACM22832.1 A C
200 ACM22990.1 A C
201 ACM23016.1 A C
202 ACM23040.1 A C
203 ACM23081.1 A C
204 ACM23138.1 A C
205 ACM23254.1 A A
206 ACM23323.1 A C
207 ACM23341.1 A C
208 ACM23415.1 A C
209 ACM23510.1 A C
210 ACM23571.1 B A
211 ACM23575.1 B B
212 ACM23847.1 A C
213 ACM23859.1 A C
214 ACM23950.1 B A
215 ACM23957.1 A C
216 ACM24018.1 B A
217 Thermotoga petrophilia ABQ46309.1 B B
218 Thermus amyloliquefaciens WP_038055695.1 A C
219 Thermus filiformis WP_038062097.1 A C
220 Thermus thermophilus WP_096411270.1 A C
221 AAS82270.1 B B
222 Truepera radiovictrix WP_013176662.1 A C
실시예 4: 복합효소(다중효소) 반응을 통한 싸이코스 생산 활성 분석
말토덱스트린으로부터 싸이코스 생산을 위해 및 7개의 효소를 선별하여 동시에(one-pot) 반응시켰다. 7개 효소는 각각 전분을 분해하여 포도당-1-인산으로 전환하는 글루칸 포스포릴레이즈와 전분을 탈분지화하는 풀루라네이즈, 전분 기질 활용도를 높이는 4-알파-글루카노트렌스퍼레이즈, 포도당-1-인산을 포도당-6-인산으로 전환하는 포스포글루코뮤테이즈, 포도당-6-인산을 과당-6-인산으로 전환하는 글루코스-6-포스페이트 아이소머레이즈, 과당-6-인산을 싸이코스-6-인산으로 전환하는 싸이코스-6-포스페이트-3-에피머레이즈와 싸이코스-6-인산으로부터 싸이코스를 생산할 수 있는 서열 1 내지 222 효소를 각각 준비하여 0.1 unit/ml을 사용하였고, 1~5 mM MgCl2, 10~50 mM의 인산나트륨(Sodium phosphate pH 7.0)에 5 %(w/v) 말토덱스트린을 첨가하여 온도 50℃에서 12시간 반응 후 HPLC를 이용하여 반응 결과물에서 싸이코스를 HPLC를 이용하여 분석하였다. HPLC 분석은 Aminex HPX-87C(Bio-RAD사) 컬럼을 사용하여 80℃에서 이동상으로 0.6 ml/min 유속으로 흘려 주면서 수행하였으며, Refractive Index Detector로 검출하였다.
그 결과, 복합효소반응에 의하여 말토덱스트린으로부터 싸이코스가 생성됨을 확인하였다.
이상의 설명으로부터, 본 출원이 속하는 기술분야의 당업자는 본 출원이 그 기술적 사상이나 필수적 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 이와 관련하여, 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적인 것이 아닌 것으로 이해해야만 한다. 본 출원의 범위는 상기 상세한 설명보다는 후술하는 특허 청구범위의 의미 및 범위 그리고 그 등가 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 출원의 범위에 포함되는 것으로 해석되어야 한다.
한국미생물보존센터(KCCM) KCCM12390P 20181114 한국미생물보존센터(KCCM) KCCM12391P 20181114 한국미생물보존센터(KCCM) KCCM12392P 20181114 한국미생물보존센터(KCCM) KCCM12393P 20181114 한국미생물보존센터(KCCM) KCCM12394P 20181114 한국미생물보존센터(KCCM) KCCM12395P 20181114 한국미생물보존센터(KCCM) KCCM12396P 20181114 한국미생물보존센터(KCCM) KCCM12397P 20181114 한국미생물보존센터(KCCM) KCCM12398P 20181114 한국미생물보존센터(KCCM) KCCM12399P 20181114 한국미생물보존센터(KCCM) KCCM12400P 20181114 한국미생물보존센터(KCCM) KCCM12401P 20181114 한국미생물보존센터(KCCM) KCCM12402P 20181114 한국미생물보존센터(KCCM) KCCM12403P 20181114 한국미생물보존센터(KCCM) KCCM12404P 20181114 한국미생물보존센터(KCCM) KCCM12405P 20181114 한국미생물보존센터(KCCM) KCCM12406P 20181114 한국미생물보존센터(KCCM) KCCM12407P 20181114
<110> CJ CheilJedang Corporation <120> A novel psicose-6-phosphatase, a composition for producing psicose comprising the same, and a method for producing psicose using the same <130> KPA181162-KR <160> 222 <170> KoPatentIn 3.0 <210> 1 <211> 265 <212> PRT <213> Alicyclobacillus acidocaldarius <400> 1 Met Asp Lys Ala Trp Leu Asp Gly Leu Ala Glu Val Val Arg Glu Ala 1 5 10 15 Gly Arg Leu Val Glu Glu Ile Ala Arg Gln Gly Phe Asp Thr Gln Phe 20 25 30 Lys His Pro Glu Glu Arg Arg Asp Pro Val Thr Thr Ala Asp Leu Ala 35 40 45 Cys Asp Ala Phe Leu Lys Glu Arg Leu Leu Thr Leu Leu Pro Glu Ala 50 55 60 Gly Trp Leu Ser Glu Glu Thr Lys Asp Arg Pro Asp Arg Leu Glu Lys 65 70 75 80 Arg Trp Val Trp Ile Val Asp Pro Ile Asp Gly Thr Arg Glu Phe Val 85 90 95 Arg Arg Ile Pro Glu Tyr Ala Ile Ser Val Ala Leu Ala Arg Asp Gly 100 105 110 Glu Pro Val Ala Gly Ala Val Val Asn Pro Ala Thr Gly Asp Leu Phe 115 120 125 Leu Gly Ala Val Gly Val Gly Ala Trp Arg Asn Gly Thr Pro Met Val 130 135 140 Cys Ser Arg Ile Arg Gly Glu Arg Leu Thr Ile Leu Gly Ser Arg Ser 145 150 155 160 Glu Met Asn Arg Gly Glu Phe Glu Pro Phe Ala Gly Ile Leu Glu Val 165 170 175 Arg Ala Val Gly Ser Ile Ala Tyr Lys Leu Ala Leu Val Ala Ala Gly 180 185 190 Glu Ala Asp Gly Thr Phe Ser Leu Gly Pro Lys His Glu Trp Asp Ile 195 200 205 Ala Ala Gly Val Ala Leu Val Leu Ala Ala Gly Gly Arg Val His Asp 210 215 220 Gly Ala Gly Arg Pro Phe Arg Phe Asn Gln Pro His Thr Leu Thr Arg 225 230 235 240 Gly Ile Val Ala Ala Thr Arg Glu Ala Tyr Gly Asp Leu Ala Leu Leu 245 250 255 Ile Glu Arg His Ala Pro Arg Arg Ala 260 265 <210> 2 <211> 262 <212> PRT <213> Alicyclobacillus tengchongensis <400> 2 Met Asn Gln Asp Val Tyr Thr Arg Leu Ala Asn Cys Asn Leu Phe Ile 1 5 10 15 Phe Asp Leu Asp Gly Thr Val Tyr Ala Glu Thr Asp His Phe Glu His 20 25 30 Tyr Leu Gln Gln Leu Ala Arg Phe Val Gly Asp Glu Ala Arg Glu Ala 35 40 45 Phe Leu Arg Glu Ala Gln Glu Ser Leu Ala Glu Gln Gly Gly Arg Phe 50 55 60 Tyr Gly Gln Ala Phe Thr Arg Leu Thr Gly Glu Pro Val Ala Val Glu 65 70 75 80 Asp Glu Pro Ser Glu Gln His Leu His Val Asp Asp Pro Trp Gly Val 85 90 95 Leu Gln Ala Val Ala Ala Arg His Gly Val Ser Val Thr Asp Arg Asp 100 105 110 Lys Ala Phe Leu Arg Thr Arg Asp Phe Met Ala Gly Pro Phe Pro Met 115 120 125 Thr Pro Leu Ala Gly Leu Arg Asp Ala Met Leu Ala Leu Arg Ser Glu 130 135 140 Gly Arg His Val Val Leu Ala Thr Asn Ser Pro Glu Pro Asp Ser Arg 145 150 155 160 Ala Ile Leu Arg Lys Leu Glu Leu Glu Asp Val Leu Glu Asp Tyr Val 165 170 175 Phe Gln Ala Arg Lys Pro Tyr Arg Thr Ala Glu His Phe Thr Arg Trp 180 185 190 Leu Ala His Tyr Asp Ile Pro Pro Glu Gln Ala Val Ser Val Gly Asp 195 200 205 His Tyr Arg Asn Glu Met Arg Pro Ala Ile Arg Leu Gly Met Asn Thr 210 215 220 Ile Tyr Ile Asp Arg Tyr Ile Gly Gln Pro Arg Ala Asp Val Thr Val 225 230 235 240 Gln Leu Arg His Pro Gly Glu Leu Ala Asp Val Leu Arg Arg Ser Leu 245 250 255 Arg Ser Ala Ala Ala Glu 260 <210> 3 <211> 218 <212> PRT <213> Alicyclobacillus tengchongensis <400> 3 Met Arg Gly Ile Val Phe Asp Phe Asp Gly Thr Leu Ile Asp Thr Glu 1 5 10 15 Ser Ala Trp Tyr Thr Ala Phe Cys Asp Leu Tyr Gln Gln His Arg Val 20 25 30 Glu Leu Pro Leu Ser Leu Tyr Ala Gln Cys Ile Gly Thr Thr His Asp 35 40 45 Ala Phe Asp Pro Val Ala His Leu Ile Ala Ser Ala Glu Met Pro Ile 50 55 60 Thr Glu Ser Glu Ile Glu Gln Phe Ile Gln Arg Arg His His Glu Leu 65 70 75 80 Met Arg Glu Lys Ser Leu Arg Pro Gly Val Lys Lys Thr Ile Glu Thr 85 90 95 Ala Arg Thr Leu Gly Leu Arg Ile Gly Leu Ala Thr Ser Ser His Arg 100 105 110 Ala Trp Ile Asp Pro Phe Leu Glu Arg Leu Gly Ile Ala Asp Ala Phe 115 120 125 His Cys Ile Arg Thr Ala Asp Asp Val Lys Arg Val Lys Pro Asp Pro 130 135 140 Ala Leu Tyr Glu Leu Thr Leu Ala Cys Leu Gly Leu Lys Pro Asp Glu 145 150 155 160 Ala Val Ala Ile Glu Asp Ser Pro Asn Gly Ala Leu Ala Ala Val Arg 165 170 175 Ala Gly Met His Val Leu Cys Thr Pro Asn Asp Val Thr Arg Gln Leu 180 185 190 Pro Phe Pro Glu Asn Cys Asn His Val Ser Ser Leu Asp Glu Ile Asp 195 200 205 Trp Pro Thr Trp Leu Gln Glu Arg Asn Arg 210 215 <210> 4 <211> 271 <212> PRT <213> Amycolatopsis thermoflava <400> 4 Met Glu Lys Pro Leu Leu Ile Ala Ser Asp Val Asp Gly Thr Leu Leu 1 5 10 15 Asp Pro Met Glu Arg Met Ser Pro Arg Thr Ala Ala Val Leu Arg Arg 20 25 30 Ala Thr Asp Ala Gly Ile Pro Val Val Leu Ala Thr Gly Arg Pro Pro 35 40 45 Arg Trp Ile Pro Ser Val Ala Gln Ala Gly Gly Leu Thr Gly Tyr Ala 50 55 60 Val Cys Ala Asn Gly Ala Val Leu Tyr Asp Ile Gly Ala Asp Glu Val 65 70 75 80 Val Gly Val His Gly Ala Leu Glu Pro Met Met Leu Asn Asp Ala Val 85 90 95 His Glu Leu Thr Lys Ala Leu Pro Gly Ala Gly Phe Ala Ala Glu Arg 100 105 110 Val Gly Arg Arg Ala Leu Asp Pro Asp Val Glu His Leu Val Val Glu 115 120 125 His Gly Tyr His Asn Pro Trp Gly Asp Gly Glu Gly Val Pro Val Ser 130 135 140 Arg Ala Glu Ile Val Gly Arg Pro Ala Val Lys Leu Leu Val Ser His 145 150 155 160 Pro Asp Met Thr Ser Asp Glu Met Ala Arg Ala Ala Arg Ala Val Leu 165 170 175 Asp Glu Ser Val Asp Ile Thr Phe Ser Ala Ser Gly Gly Leu Ile Glu 180 185 190 Ile Ala Ala His Gly Ile Thr Lys Ala Thr Gly Leu Ala Glu Val Ala 195 200 205 Glu Arg Phe Gly Val Pro Val Glu Arg Ile Ile Ala Phe Gly Asp Met 210 215 220 Pro Asn Asp Leu Glu Met Leu Lys Trp Ala Gly His Gly Val Ala Met 225 230 235 240 Ala Asn Ala His Pro Asp Val Leu Ala Ile Ala Asp Glu Val Thr Ser 245 250 255 Pro Asn Ser Glu Asp Gly Val Ala Gln Val Leu Glu Arg Trp Phe 260 265 270 <210> 5 <211> 220 <212> PRT <213> Amycolatopsis thermoflava <400> 5 Met Ala Lys Val Leu Val Leu Asp Val Asp Gly Thr Leu Val Asp Thr 1 5 10 15 Asn Tyr His His Ala Leu Ala Trp Phe Arg Ala Phe Arg Gly His Gly 20 25 30 Val Thr Val Pro Val Trp Arg Leu His Arg Ala Ile Gly Met Gly Gly 35 40 45 Asp Gln Leu Val Pro Glu Val Ala Gly Gln Glu Val Glu Asp Ser Cys 50 55 60 Gly Asp Asp Val Arg Ala Lys Trp Lys Glu Leu Val Asp Gly Met Leu 65 70 75 80 Pro Glu Val Cys Ala Leu Asp Gly Ala His Glu Leu Leu Gln Ala Ala 85 90 95 Asp Asp Ala Gly Tyr Arg Val Val Leu Ala Ser Ser Gly Lys Pro Asp 100 105 110 His Val Asp His Tyr Leu Asp Leu Ile Asp Gly Arg Glu Leu Ala Ala 115 120 125 Asp Trp Thr Ser Ser Lys Asp Val Glu Ala Thr Lys Pro Glu Pro Asp 130 135 140 Leu Leu Glu Val Ala Leu Glu Lys Val Arg Gly Glu Glu Ala Val Val 145 150 155 160 Ile Gly Asp Ser Val Trp Asp Cys Val Ala Ala Arg Arg Ile Gly Leu 165 170 175 Pro Cys Val Gly Leu Leu Thr Gly Gly Phe Ser Ala Ala Glu Leu Thr 180 185 190 Asp Ala Gly Ala Ala Arg Thr Phe Thr Asp Leu Arg Glu Leu Arg Ala 195 200 205 Ser Leu Asp Glu Leu Pro Phe Gly Ala Ser Pro Arg 210 215 220 <210> 6 <211> 406 <212> PRT <213> Anaerolinea thermolimosa <400> 6 Met Val Lys Arg Glu Val Leu Ala Ile Ile Pro Ala Arg Gly Gly Ser 1 5 10 15 Lys Gly Ile Pro Arg Lys Asn Val Arg Leu Phe Ala Gly His Pro Leu 20 25 30 Ile Ala Tyr Ser Ile Ala Ala Ala Arg Gln Ser Glu Leu Val Thr Arg 35 40 45 Val Ile Val Ser Thr Asp Asp Glu Glu Ile Ala Arg Val Ala Arg Asp 50 55 60 Tyr Gly Ala Glu Thr Pro Phe Leu Arg Pro Ala Glu Leu Ala Gln Asp 65 70 75 80 Gln Thr Leu Asp Leu Pro Val Phe Gln His Ala Leu Arg Trp Leu Glu 85 90 95 Glu Gln Glu Gly Tyr Gln Pro Glu Leu Val Val Gln Leu Arg Pro Thr 100 105 110 Ser Pro Val Arg Pro Pro Ala Leu Val Asp Glu Ala Val Gln Ile Leu 115 120 125 Leu Ala His Pro Glu Ala His Ser Val Arg Gly Val Val Pro Ala Gly 130 135 140 Gln Asn Pro His Lys Met Trp Arg Ile Asp Pro Gln Asn Gly Gln Met 145 150 155 160 Ile Pro Leu Leu Gln Val Pro Gly Leu Glu Glu Pro Tyr Asn Ala Pro 165 170 175 Arg Gln Val Leu Pro Pro Val Tyr Trp Gln Thr Gly His Ile Asp Val 180 185 190 Ile Arg Pro Glu Val Ile Arg Lys Gly Ser Met Ser Gly Gln Val Ile 195 200 205 Leu Pro Val Met Val Asp Pro Ala Tyr Thr Val Asp Ile Asp Thr Pro 210 215 220 Arg Asp Trp Ala Arg Ser Glu Trp Leu Val Trp Tyr Ser Glu Leu Glu 225 230 235 240 Ile Val Tyr Pro Gly His Arg Arg Arg Pro Leu Pro Glu Lys Val Asp 245 250 255 Leu Val Val Phe Asp Phe Asp Gly Val Leu Thr Asp Asn Arg Val Trp 260 265 270 Val Asp Glu Glu Gly His Glu Met Val Ala Ala Asn Arg Ser Asp Ser 275 280 285 Leu Gly Val Ala Tyr Leu Arg Arg Ser Gly Leu Lys Met Val Val Leu 290 295 300 Ser Thr Glu Thr Asn Pro Val Val Ala Ala Arg Cys Arg Lys Met Gln 305 310 315 320 Ile Pro Val Val Gln Gly Val Trp Asp Lys Ala Gly Glu Leu Pro Arg 325 330 335 Ile Met Ala Glu Phe Asn Ala Ala Pro Glu Arg Thr Ile Tyr Val Gly 340 345 350 Asn Asp Val Asn Asp Val Pro Cys Phe Ser Leu Val Gly Cys Ala Val 355 360 365 Ala Val Ala Asp Ala Gln Pro Ala Ala Arg Arg Ala Ala Asp Ile Val 370 375 380 Leu Ser Arg Asn Gly Gly His Gly Ala Val Arg Glu Leu Leu Asp Ile 385 390 395 400 Leu Met Gln Arg Met Lys 405 <210> 7 <211> 226 <212> PRT <213> Anaerolinea thermolimosa <400> 7 Met Gly Ile Asp Arg Gln Arg Val Cys Gly Ile Cys Phe Asp Val Asp 1 5 10 15 Gly Thr Leu Ser Asp Thr Asp Asp Leu Trp Val Ala Arg Leu Ser Gly 20 25 30 Trp Leu Lys Pro Val Ser Trp Ile Phe Pro Gln Arg Asn Pro Ser Arg 35 40 45 Phe Ala Arg Trp Ala Ile Met Ala Ala Glu Ser Pro Gly Asn Leu Val 50 55 60 Tyr Ser Leu Phe Asp Arg Phe Gly Leu Asp Asp Glu Ile Gly Arg Leu 65 70 75 80 Phe His Trp Leu Asn Arg Gln Arg Gly Val Leu Arg Ser Gln Arg Phe 85 90 95 Leu Leu Ile Pro Gly Val Gln Glu Ala Leu His Arg Leu His Lys His 100 105 110 Phe Pro Leu Ala Val Val Ser Ala Arg Asp His Ala Gly Thr Leu Ala 115 120 125 Phe Leu Gln His Phe Glu Leu Glu Pro Phe Phe Arg Cys Val Ala Thr 130 135 140 Ser Gln Thr Cys Glu Phe Thr Lys Pro Phe Pro His Pro Ile Arg Trp 145 150 155 160 Ala Ala Ala Gln Met Gly Val Lys Pro Glu Glu Leu Leu Met Val Gly 165 170 175 Asp Thr Val Val Asp Ile Arg Ala Gly Lys Ala Ala Gly Ala Gln Thr 180 185 190 Val Gly Val Leu Cys Gly Phe Gly Gln Glu Glu Glu Leu Arg Arg Ala 195 200 205 Gly Ala Asp Leu Ile Leu Pro His Thr Ala Gln Leu Ala Asp Val Leu 210 215 220 Leu Asp 225 <210> 8 <211> 222 <212> PRT <213> Anaerolinea thermolimosa <400> 8 Met His Phe Ser Ala Tyr Ile Phe Asp Met Asp Gly Thr Leu Ile Asp 1 5 10 15 Asn Met Gly Phe His Gly Ala Ile Trp Ser Glu Phe Leu Ala Ser Leu 20 25 30 Gly Ala Pro Val Asp Arg Glu Thr Phe Phe Arg Arg Thr Val Gly Lys 35 40 45 Val Asn Ala Glu Ile Leu Arg Asp Leu Tyr Arg Ala Asp Leu Ser Asp 50 55 60 Ala Glu Ile Glu Glu Leu Ser Arg Arg Lys Glu Gln Leu Tyr Arg Gln 65 70 75 80 Arg Phe Ala Pro Leu Ile Thr Gln Lys Ala Val Pro Gly Val Arg Glu 85 90 95 Phe Leu Gln Lys Ala His Gln Arg Gly Ile Ser Met Ala Val Ala Thr 100 105 110 Ser Ala Gly Leu Glu Asn Thr Arg Phe Ile Leu Asp Gly Leu Gly Ile 115 120 125 Thr Ala Tyr Phe Ser Ala Leu Val Thr Ser Glu Asp Val His Arg Gly 130 135 140 Lys Pro Asp Pro Glu Ile Phe Leu Ile Ala Ala Gln Lys Leu Gly Val 145 150 155 160 Pro Pro Glu Ala Cys Leu Val Phe Glu Asp Ser Pro Ala Gly Leu Glu 165 170 175 Ala Ala His Arg Ala Gly Met Val Ser Val Ala Leu Ala Thr Thr Phe 180 185 190 Pro Ala Glu Gln Leu Ala Gly Arg Pro Gly Val Leu Leu Val Thr Gly 195 200 205 Asp Tyr Ser Ala Ile Asp Leu Thr Arg Leu Ile Val Gly Thr 210 215 220 <210> 9 <211> 259 <212> PRT <213> Anaerolinea thermophila <400> 9 Met Thr Thr Ser Leu Arg Asp Leu Leu Asp Phe Ala Val Glu Thr Ala 1 5 10 15 Tyr Leu Ala Gly Arg Thr Thr Leu Ala Tyr Phe Gln Thr Gly Val Gln 20 25 30 Ala Glu Phe Lys Ala Asp Ser Ser Pro Val Thr Leu Ala Asp Arg Ala 35 40 45 Ala Glu Gln Leu Ile Arg Gln Arg Ile Glu Lys Arg Phe Pro His His 50 55 60 Ala Ile Val Gly Glu Glu Phe Gly Val Gln Gly Ser Ala Asp Ala Thr 65 70 75 80 His Arg Trp Phe Ile Asp Pro Ile Asp Gly Thr Lys Ser Phe Leu Arg 85 90 95 Gly Ile Pro Leu Tyr Ala Val Leu Leu Gly Leu Glu Ile Glu Gly Arg 100 105 110 Val Gln Val Gly Val Ala Tyr Tyr Pro Ala Met Asp Glu Met Leu Ser 115 120 125 Ala Ala Asp Gly Glu Gly Cys Trp Trp Asn Gly Arg Arg Ala Arg Val 130 135 140 Ser Thr Ala Ser Arg Leu Ala Glu Ala Trp Val Thr Ser Thr Asp Pro 145 150 155 160 Tyr Asn Phe Gln Lys Thr Gly Lys Asp Ala Ala Trp Gln Arg Ile Gln 165 170 175 Ala Val Ser Tyr His Arg Gly Gly Trp Gly Asp Ala Tyr Gly Tyr Leu 180 185 190 Leu Val Ala Thr Gly Arg Ala Glu Val Met Leu Asp Pro Ile Met Asn 195 200 205 Glu Trp Asp Cys Ala Pro Phe Pro Pro Ile Phe Arg Glu Ala Gly Gly 210 215 220 Phe Phe Gly Asp Trp Gln Gly Asn Glu Thr Ile Tyr Gly Gly Glu Ala 225 230 235 240 Leu Ala Thr Thr Gln Val Leu Leu Pro Glu Val Leu Glu Cys Leu His 245 250 255 Ser Ser Leu <210> 10 <211> 223 <212> PRT <213> Anaerolinea thermophila <400> 10 Met Thr Lys Thr Asn Ile Lys Gly Ile Ile Phe Asp Phe Asp Gly Leu 1 5 10 15 Ile Leu Asp Thr Glu Thr Pro Glu Met Leu Ala Trp Glu Lys Ala Phe 20 25 30 Thr Glu His Ser Leu Ser Phe Pro Ile Glu Arg Tyr Leu Asn Ser Ile 35 40 45 Gly Thr Val Ser Asp Asn His Phe Val Gln Gly Phe Met Gln Glu Met 50 55 60 Gly Leu Ser Glu Gly Glu Ile Gln Gln Thr Val Leu Ala Tyr Gln Ser 65 70 75 80 His Ile Ala Thr Phe Glu His Met Asn His Pro Arg Glu Gly Val Leu 85 90 95 Asp Leu Ile His Asp Ala Glu Lys Ile Gly Leu Arg Leu Ala Val Ala 100 105 110 Ser Asn Ser Tyr Arg Thr Trp Val Val Asp Asn Leu Gln Arg Leu Ala 115 120 125 Leu Asp Ser His Phe Asp Pro Ile Cys Thr Arg Asp Asp Val Thr Asn 130 135 140 Ser Lys Pro Asp Pro Glu Leu Tyr Asn Leu Val Leu Ala Lys Trp Gly 145 150 155 160 Phe Thr Ser Asn Glu Val Leu Ala Leu Glu Asp Ser Pro Asn Gly Ile 165 170 175 Lys Ala Ala Lys Asn Ala Gly Ile Phe Cys Val Ala Val Pro Asn Pro 180 185 190 Ile Thr Ile Arg Met Asp Val Thr Leu Ala Asp Leu Ile Phe Ser Ser 195 200 205 Phe Ser Asp Phe Ser Leu Gln Glu Ile Leu Phe Gln Leu Glu Lys 210 215 220 <210> 11 <211> 232 <212> PRT <213> Anaerolinea thermophila <400> 11 Met Pro Leu Asp Val Ser Arg Ile Gln Ala Ile Cys Phe Asp Ile Asp 1 5 10 15 Gly Thr Leu Ser Asp Thr Asp Asp Glu Trp Ala Glu Arg Val Leu Lys 20 25 30 Gly Ile Ser Phe Leu Lys Gly Trp Val Gly Glu Gln Arg Leu Arg Asn 35 40 45 Leu Ala Arg Trp Val Val Met Glu Ile Glu Ser Pro Gly Asn Leu Leu 50 55 60 Tyr Asn Leu Leu Asp Arg Leu His Leu Asp Asp Glu Ala Ser Arg Leu 65 70 75 80 Phe Asn Phe Phe Val Arg Lys Phe His Pro Arg Pro Ser Arg Phe Arg 85 90 95 Leu Ile Ser Gly Met Asp Lys Thr Leu Gln Thr Leu Ala Gln Tyr Tyr 100 105 110 Pro Leu Ala Ile Val Ser Ala Arg Asp Glu Glu Ala Thr Leu Ala Phe 115 120 125 Leu Gln Gln Phe Asp Leu Thr Ser Leu Phe Gln Val Ile Val Ser Ala 130 135 140 His Thr Cys Lys Phe Thr Lys Pro Phe Pro Asp Pro Ile Leu Trp Ala 145 150 155 160 Ala Glu Lys Leu Asn Val Pro Pro Ser Ala Cys Leu Met Val Gly Asp 165 170 175 Thr Thr Val Asp Ile His Ala Gly Lys Lys Ala Gly Ala Gln Thr Val 180 185 190 Gly Val Leu Cys Gly Phe Gly Thr His Lys Glu Leu Val Cys Ala Gly 195 200 205 Ala Asp Leu Ile Leu Glu Thr Thr Ser Glu Leu Ala Glu Ile Leu Leu 210 215 220 Lys Glu Lys Asn Ser Met Pro Asn 225 230 <210> 12 <211> 252 <212> PRT <213> Archaeoglobus fugidus <400> 12 Met Asp Glu Arg Asp Ala Leu Arg Ile Ser Arg Glu Ile Ala Gly Glu 1 5 10 15 Val Arg Lys Ala Ile Ala Ser Met Pro Leu Arg Glu Arg Val Lys Asp 20 25 30 Val Gly Met Gly Lys Asp Gly Thr Pro Thr Lys Ala Ala Asp Arg Val 35 40 45 Ala Glu Asp Ala Ala Leu Glu Ile Leu Arg Lys Glu Arg Val Thr Val 50 55 60 Val Thr Glu Glu Ser Gly Val Leu Gly Glu Gly Asp Val Phe Val Ala 65 70 75 80 Leu Asp Pro Leu Asp Gly Thr Phe Asn Ala Thr Arg Gly Ile Pro Val 85 90 95 Tyr Ser Val Ser Leu Cys Phe Ser Tyr Ser Asp Lys Leu Lys Asp Ala 100 105 110 Phe Phe Gly Tyr Val Tyr Asn Leu Ala Thr Gly Asp Glu Tyr Tyr Ala 115 120 125 Asp Ser Ser Gly Ala Tyr Arg Asn Gly Glu Arg Ile Glu Val Ser Asp 130 135 140 Ala Glu Glu Leu Tyr Cys Asn Ala Ile Ile Tyr Tyr Pro Asp Arg Lys 145 150 155 160 Phe Pro Phe Lys Arg Met Arg Ile Phe Gly Ser Ala Ala Thr Glu Leu 165 170 175 Cys Phe Phe Ala Asp Gly Ser Phe Asp Cys Phe Leu Asp Ile Arg Pro 180 185 190 Gly Lys Met Leu Arg Ile Tyr Asp Ala Ala Ala Gly Val Phe Ile Ala 195 200 205 Glu Lys Ala Gly Gly Lys Val Thr Glu Leu Asp Gly Glu Ser Leu Gly 210 215 220 Asn Lys Lys Phe Asp Met Gln Glu Arg Leu Asn Ile Val Ala Ala Asn 225 230 235 240 Glu Lys Leu His Pro Lys Leu Leu Glu Leu Ile Lys 245 250 <210> 13 <211> 344 <212> PRT <213> Archaeoglobus fugidus <400> 13 Met Phe Val Met Phe Ile Val Ala Glu Val Asp Asp Glu Val Cys Val 1 5 10 15 Lys Arg Glu Val Glu Arg Ala Ala Gly Glu Val Gly Val His Val Ser 20 25 30 Leu Thr Pro Phe Gln Arg Arg Glu Lys Ala Glu Lys Asn Leu Tyr Val 35 40 45 Val Thr Ile Leu Gly Lys Asp Arg Val Gly Ile Val Arg Asp Ile Thr 50 55 60 Arg Ala Phe Leu Asp Phe Gly Ile Asn Ile Glu Arg Thr Ser Leu Thr 65 70 75 80 Ala Arg Glu Glu Leu Ile Ser Ile Glu Phe Leu Val Asp Leu Gly Gln 85 90 95 Arg Asp Ala Ala Glu Val Arg Lys Arg Leu Arg Arg Glu Ala Glu Arg 100 105 110 Leu Gly Leu Asp Ile Val Met Gln Pro Tyr Ser Thr Phe Asn Arg Glu 115 120 125 Lys Arg Leu Ile Val Phe Asp Met Asp Ser Thr Leu Val Glu Ala Glu 130 135 140 Ile Ile Asp Glu Leu Ala Lys Glu Ala Gly Val Gly Asp Glu Val Ser 145 150 155 160 Lys Leu Thr Glu Arg Ala Met Arg Gly Glu Ile Gly Phe Lys Glu Ala 165 170 175 Leu Glu Glu Arg Val Arg Leu Leu Lys Gly Leu Pro Val Glu Val Leu 180 185 190 Glu Arg Ile Tyr Ser Arg Ile Lys Leu Thr Glu Gly Ala Lys Glu Leu 195 200 205 Val Arg Ser Leu Lys Glu Ala Gly Tyr Lys Val Ala Val Val Ser Gly 210 215 220 Gly Phe Ser Tyr Phe Thr Asp Arg Leu Lys Glu Glu Leu Gly Leu Asp 225 230 235 240 Tyr Ala Phe Gly Asn Glu Leu Glu Ile Glu Asn Gly Arg Leu Thr Gly 245 250 255 Arg Ile Lys Gly Arg Ile Ile Asp Ala Ser Glu Lys Ala Arg Ile Val 260 265 270 Glu Glu Ile Ala Arg Lys Glu Gly Ile Ser Pro Glu Asn Val Val Ala 275 280 285 Val Gly Asp Gly Ala Asn Asp Arg Leu Met Ile Glu Arg Ala Gly Leu 290 295 300 Gly Ile Ala Phe Asn Ala Lys Glu Val Leu Lys Asp Val Ala Asp Gly 305 310 315 320 Ser Ile Ser Lys Glu Asn Leu Val Gly Leu Ala Ser Val Leu Lys Leu 325 330 335 Pro Ala Glu Phe Arg Lys Lys Val 340 <210> 14 <211> 277 <212> PRT <213> Archaeoglobus fugidus <400> 14 Met Leu Lys Thr Pro Ser Asp Ala Ala Val Ala Gly Cys Leu Ser Leu 1 5 10 15 Leu Leu Glu Val Ser Gly Asn Pro Lys Ala Gly Asn Val Asp Arg Glu 20 25 30 His Asp Phe Asp Asp Leu Lys Phe Glu His Phe Leu Ala Ser Ala Ala 35 40 45 Gly Ala Phe Pro Ala Phe Leu Glu Val Ala Glu Lys Arg Ile Ile Gly 50 55 60 Glu Gly Val Leu Arg Ala Val Lys Glu Ser Met Arg Trp His Arg Ala 65 70 75 80 Glu Asn Val His Phe Gly Ala Phe Leu Leu Leu Val Pro Leu Ile Ser 85 90 95 Ser Trp Asp Ala Gly Gly Met Val Asp Ile Ala Glu Ala Ala Arg Asn 100 105 110 Arg Leu Arg Arg Thr Asp Phe Arg Asp Ser Leu Ser Val Leu Glu Ala 115 120 125 Phe Arg Leu Ser Asn Ala Arg Val Val Glu Ala Gly Glu Leu Asn Leu 130 135 140 Lys Asp Arg Lys Thr Glu Glu Glu Ile Ala Gln Lys Lys Ile Asn Leu 145 150 155 160 Tyr Glu Trp Met Lys Met Ala Pro Glu Glu Asn Leu Ile Ala Arg Glu 165 170 175 Leu Val Asp Gly Phe Lys Ile Ser Ile Glu Gly Ala Lys Phe Leu Leu 180 185 190 Ser Phe Gly Asn Ser Gly Lys Ala Val Val Glu Leu Tyr Tyr His Leu 195 200 205 Leu Ser Lys Phe Pro Asp Pro Leu Val Ile Ala Lys Met Gly Arg Glu 210 215 220 Tyr Ala Glu Lys Ile Thr Glu Trp Ala Glu Lys Ala Arg Thr Glu Glu 225 230 235 240 Glu Arg Lys Glu Leu Asp Glu Lys Leu Leu Lys Asp Gly Ala Asn Pro 245 250 255 Gly Thr Ile Ala Asp Leu Thr Ala Ser Ser Ile Phe Leu Ala Leu Ala 260 265 270 Glu Gly Trp Arg Ile 275 <210> 15 <211> 219 <212> PRT <213> Archaeoglobus profundus <400> 15 Met Phe Lys Ala Leu Val Val Asp Ile Asp Gly Thr Leu Thr Asp Lys 1 5 10 15 Lys Arg Ala Ile Asn Cys Arg Ala Val Glu Ala Leu Arg Lys Leu Lys 20 25 30 Ile Pro Val Val Leu Ala Thr Gly Asn Ile Ser Cys Phe Ala Arg Ala 35 40 45 Val Ala Lys Ile Ile Gly Val Ser Asp Ile Val Ile Ala Glu Asn Gly 50 55 60 Gly Val Val Arg Phe Ser Tyr Asp Gly Glu Asp Ile Val Leu Gly Asp 65 70 75 80 Arg Ser Lys Cys Leu Arg Ala Leu Glu Thr Leu Arg Lys Arg Phe Lys 85 90 95 Val Glu Leu Leu Asp Asn Glu Tyr Arg Lys Ser Glu Val Cys Met Arg 100 105 110 Arg Asn Phe Pro Ile Glu Glu Ala Arg Lys Ile Leu Pro Lys Asp Val 115 120 125 Arg Ile Val Asp Thr Gly Phe Ala Tyr His Ile Ile Asp Ala Asn Val 130 135 140 Ser Lys Gly Lys Ala Leu Met Phe Ile Ala Asp Lys Leu Gly Leu Asp 145 150 155 160 Val Lys Asp Phe Ile Ala Ile Gly Asp Ser Glu Asn Asp Ile Glu Met 165 170 175 Leu Glu Val Ala Gly Phe Gly Val Ala Val Ala Asn Ala Asp Glu Lys 180 185 190 Leu Lys Glu Val Ala Asp Leu Val Thr Ser Lys Pro Asn Gly Asp Gly 195 200 205 Val Val Glu Ala Leu Glu Phe Leu Gly Leu Ile 210 215 <210> 16 <211> 224 <212> PRT <213> Archaeoglobus veneficus <400> 16 Met Leu Arg Pro Lys Gly Leu Ala Ile Asp Ile Asp Gly Thr Ile Thr 1 5 10 15 Tyr Arg Asn Arg Ser Leu Asn Cys Lys Ala Val Glu Ala Leu Arg Lys 20 25 30 Val Lys Ile Pro Val Val Leu Ala Thr Gly Asn Ile Ser Cys Phe Ala 35 40 45 Arg Thr Ala Ala Lys Leu Ile Gly Val Ser Asp Ile Val Ile Cys Glu 50 55 60 Asn Gly Gly Ile Val Arg Phe Ser Tyr Asp Gly Asp Asp Ile Val Leu 65 70 75 80 Gly Asp Ile Ser Lys Cys Leu Lys Ala Ala Glu Ile Leu Lys Glu Tyr 85 90 95 Phe Glu Ile Glu Phe Leu Asp Ala Glu Tyr Arg Lys Ser Glu Val Cys 100 105 110 Leu Arg Arg Asn Phe Pro Ile Glu Glu Ala Arg Lys Ile Leu His Asp 115 120 125 Ala Lys Leu Asp Val Lys Ile Val Asp Ser Gly Phe Ala Tyr His Ile 130 135 140 Met Asp Ala Lys Val Ser Lys Gly Arg Ala Leu Glu Tyr Ile Ala Asp 145 150 155 160 Glu Leu Gly Ile Ser Pro Lys Glu Phe Ala Ala Ile Gly Asp Ser Glu 165 170 175 Asn Asp Ile Asp Leu Ile Lys Ala Ala Gly Leu Gly Ile Ala Val Gly 180 185 190 Asp Ala Asp Leu Lys Leu Lys Met Glu Ala Asp Val Val Val Ser Lys 195 200 205 Lys Asn Gly Asp Gly Val Val Glu Ala Leu Glu Leu Leu Gly Leu Ile 210 215 220 <210> 17 <211> 194 <212> PRT <213> Bacillus licheniformis <400> 17 Met Leu His Leu Gly Ile Asp Ile Asp Gly Thr Ile Thr Ala Gln Asp 1 5 10 15 Thr Phe Val Pro Tyr Leu Asn Glu Ser Phe Ser Leu Ser Ile Thr Leu 20 25 30 Glu Asp Ile Lys Glu Tyr Asp Leu Thr Lys Leu Leu Asn Ile Ser Gln 35 40 45 Glu Asp Phe Trp Lys Trp Met Asn Glu Asn Glu Pro Arg Ile Tyr Gln 50 55 60 Glu Ala Leu Leu Ala Gln Tyr Ala Lys Gln Thr Leu Asp Glu Leu Lys 65 70 75 80 Lys Gln His Lys Leu Ile Tyr Ile Thr Ala Arg Arg His His Leu Glu 85 90 95 Asp Ile Thr Tyr Asp Trp Phe Glu Lys Arg Asp Ile His Tyr Asp His 100 105 110 Ile Glu Leu Val Gly Gly His Asp Lys Leu Glu Ala Val Phe Arg His 115 120 125 Glu Ile Asp Val Phe Phe Glu Asp His His Gly Asn Ala Thr Met Ile 130 135 140 Ala Lys Glu Ala Gly Ile Pro Val Leu Leu Phe Asn Thr Pro Tyr Asn 145 150 155 160 Gln Leu Pro Thr Asp Ser Asn Ile Val Arg Val Asp Ser Trp Leu Glu 165 170 175 Ala Ala Glu Trp Leu Lys Lys Asn Glu Lys Thr Leu Lys Ala Ala Lys 180 185 190 Ser Met <210> 18 <211> 226 <212> PRT <213> Bacillus licheniformis <400> 18 Met Lys Ala Val Ile Phe Asp Leu Asp Gly Val Ile Thr Asp Thr Ala 1 5 10 15 Glu Tyr His Tyr Leu Ala Trp Lys His Thr Ala Glu Gln Ile Gly Ile 20 25 30 Glu Ile Asp Arg Ser Phe Asn Glu Arg Leu Lys Gly Ile Asn Arg Glu 35 40 45 Gln Ser Leu Asp Lys Ile Leu Ile His Gly Gly Ala Ala Gly Lys Phe 50 55 60 Gln Glu Ala Glu Lys Gln Glu Ile Met Arg Arg Lys Asn Gln Tyr Tyr 65 70 75 80 Gln Gln Leu Ile Gln Asn Leu Thr Pro His Asp Leu Leu Pro Gly Ile 85 90 95 Ser Val Leu Phe Ala Glu Leu Lys Arg Glu His Ile Ser Ile Ala Leu 100 105 110 Ala Ser Ser Ser Arg Asn Ala Pro Ala Ile Leu Gln Arg Leu Gly Val 115 120 125 Met Asp Glu Phe Gln Gly Val Val Asp Pro Ala Ala Leu Ala His Gly 130 135 140 Lys Pro Asp Pro Glu Ile Phe Leu Thr Ala Ala Ala Leu Leu Gly Val 145 150 155 160 Pro Pro Ser Glu Cys Ala Ala Ile Glu Asp Ala Glu Ala Gly Ile Ala 165 170 175 Ala Ile Lys Ser Ala Gly Met Phe Ala Val Gly Val Gly Asp Glu Thr 180 185 190 Ser Leu Arg Gly Ala Asp Leu Ile Val His Asn Thr Asn Glu Leu Thr 195 200 205 Phe Glu Leu Leu Asn Glu Gly Trp Gln Arg Tyr Cys Cys Ile Arg Glu 210 215 220 Gly Lys 225 <210> 19 <211> 216 <212> PRT <213> Bacillus licheniformis <400> 19 Met Lys Lys Trp Ala Phe Val Ser Asp Phe Asp Gly Thr Ile Ser Lys 1 5 10 15 Gln Asp Phe Tyr Trp Met Val Ile Asp Lys Tyr Phe Pro Glu Gly Arg 20 25 30 Glu Leu Phe Lys Lys Trp Lys Ser Gly Glu Leu Lys Asp Ile Glu Phe 35 40 45 Leu Gly Thr Val Phe Ala Ser Ile Asn Gln Ser Glu Gln Lys Ile Ile 50 55 60 Asp Asp Ile His Ser Ile Pro Ile Asp Glu Tyr Val Pro Asp Phe Ile 65 70 75 80 Gln His Val Gln Lys Ser Gly Gly Asp Phe Tyr Ile Leu Ser Ala Gly 85 90 95 Thr Asp Tyr Tyr Ile His Tyr Ile Leu Lys Lys Tyr Gly Ile Thr Asp 100 105 110 Val Glu Val Tyr Ser Asn Lys Gly Phe Phe Lys Glu Asp Asn Val His 115 120 125 Met Asp Ile Asp Glu Asn His Trp His Tyr Ser Glu Arg Tyr Gly Ile 130 135 140 Asp Lys Ser Lys Val Ile Gln Lys Leu Lys Glu Glu Tyr Glu Thr Val 145 150 155 160 Tyr Phe Ala Gly Asp Ser Glu Pro Asp Ser His Pro Ala Lys Phe Ala 165 170 175 Asp Val Thr Phe Ala Lys Asp Ala Leu Gln Asp Leu Leu Arg Gln Gln 180 185 190 Gly Val Pro Phe Val Ala Val Glu Thr Phe Glu Asp Ile Glu Gln Tyr 195 200 205 Leu Lys Glu Lys Gly Arg Ile Val 210 215 <210> 20 <211> 223 <212> PRT <213> Caldicellulosiruptor bescii <400> 20 Met Gly Lys Ile Lys Ala Ala Ile Phe Asp Met Asp Gly Val Leu Thr 1 5 10 15 Asp Thr Val Lys Leu His Phe Lys Ala Trp Lys Lys Met Phe Glu Ser 20 25 30 His Gly Tyr Lys Phe Glu Tyr Glu Asp Tyr Lys Trp Lys Val Asp Gly 35 40 45 Lys Pro Arg Ile Asp Gly Ile Arg Ser Ile Ala Tyr Asp Met Pro Glu 50 55 60 Asp Lys Leu Ile Glu Met Ala Glu Glu Lys Gln Lys Ile Phe Leu Glu 65 70 75 80 Phe Val Glu Gln Glu Asn Leu Glu Ala Phe Glu Asp Ser Ile Trp Leu 85 90 95 Leu Asn His Leu Lys Gln Asn Asp Ile Lys Leu Ala Val Ala Ser Ser 100 105 110 Ser Lys Asn Thr Thr Lys Ile Leu Thr Lys Ile Gly Ile Tyr Asn Met 115 120 125 Phe Asp Thr Ile Val Thr Gly Tyr Asp Phe Lys Lys Gly Lys Pro Asp 130 135 140 Pro Glu Ile Phe Leu Thr Ala Ala Gln Arg Leu Asn Val Asn Pro Lys 145 150 155 160 Glu Cys Val Val Phe Glu Asp Ala Ile Asp Gly Val Lys Ala Gly Ile 165 170 175 Arg Ala Gly Met Leu Thr Ile Gly Val Cys Arg Asp Gly Gln Phe Asp 180 185 190 Arg Leu Lys Glu Ala His Tyr Val Val Asp Arg Leu Asp Lys Ile Ser 195 200 205 Leu Glu Leu Leu Glu Asn Leu His Glu Lys Leu Phe Lys Lys Val 210 215 220 <210> 21 <211> 275 <212> PRT <213> Caldicellulosiruptor bescii <400> 21 Met Lys Asn Ser Ser Ile Leu Lys Asn Ile Asp Leu Phe Leu Leu Asp 1 5 10 15 Leu Asp Gly Thr Val Tyr Leu Gly Glu Lys Val Phe Glu Gly Ala Arg 20 25 30 Glu Phe Ile Lys Leu Leu Asn Lys Asn Gln Lys Glu Phe Leu Phe Leu 35 40 45 Thr Asn Asn Ser Ser Lys Ser Ser Glu Glu Tyr Tyr Ser Lys Leu Leu 50 55 60 Asn Met Gly Phe Glu Ile Thr Lys Glu Asn Val Phe Thr Ser Gly Gln 65 70 75 80 Ala Met Gly Ile Tyr Ile Lys Thr Ile His Lys Lys Glu Lys Pro Pro 85 90 95 Arg Val Tyr Val Val Gly Thr Thr Ser Leu Lys Arg Glu Leu Lys Ser 100 105 110 Met Gly Ile Val Val Val Asp Ser Pro Asn Tyr Asn Ile Asp Tyr Leu 115 120 125 Val Ile Gly Phe Asp Thr Thr Leu Thr Tyr Lys Lys Leu Leu Asp Ala 130 135 140 Cys Glu Leu Ile Arg Arg Gly Val Pro Phe Leu Ala Thr Asn Pro Asp 145 150 155 160 Leu Val Cys Pro Leu Asp Gly Gly Arg Tyr Ile Pro Asp Cys Gly Ser 165 170 175 Ile Cys Ile Met Leu Glu Asn Ala Thr Lys Lys Lys Pro Val Phe Val 180 185 190 Gly Lys Pro Ser Ser Ile Met Val Asp Ile Ile Ser Asn Leu Lys Lys 195 200 205 Val Glu Lys Ser Arg Ile Ala Met Ile Gly Asp Arg Leu Tyr Thr Asp 210 215 220 Met Lys Met Ala Lys Asp Ser Gly Met Val Ala Ala Leu Val Leu Ser 225 230 235 240 Gly Glu Thr Lys Met Lys Asp Val Glu Ala Ser Thr Leu Lys Pro Asp 245 250 255 Leu Ile Tyr Gly Ser Ile Lys Asp Met Tyr Glu Glu Leu Lys Leu Val 260 265 270 Phe Gly Gly 275 <210> 22 <211> 266 <212> PRT <213> Caldicellulosiruptor bescii <400> 22 Met Ile Lys Leu Val Ala Thr Asp Leu Asp Asp Thr Leu Leu Ser Lys 1 5 10 15 Asp Leu Thr Ile Thr Glu Lys Asn Leu Asn Ala Ile Glu Phe Leu Lys 20 25 30 Lys Asn Asn Ile Ile Phe Ile Leu Ala Ser Gly Arg Pro Tyr Pro Ser 35 40 45 Ile Lys Asn Val Ala Tyr Asp Leu Gln Asn Phe Tyr Pro Met Ile Thr 50 55 60 Tyr Gln Gly Ala Leu Val Tyr Asp Pro Lys Asn Asp Lys Lys Leu Tyr 65 70 75 80 Gly Cys Glu Ile Lys Pro Glu Asp Ala Lys Glu Leu Val Arg Leu Ala 85 90 95 Lys Asp Glu Gly Ile His Val His Ile Tyr Ile Asp Asn Val Trp Tyr 100 105 110 Val Glu Ala Met Asn Glu Lys Thr Glu Tyr Tyr Arg Asn Leu Thr Lys 115 120 125 Leu Glu Pro His Ile Val Lys Asn Leu Leu Glu Phe Ile Asp Arg Pro 130 135 140 Val Thr Lys Val Leu Phe Phe Asp Glu His Glu Arg Leu Lys Asp Leu 145 150 155 160 Lys Glu Ser Leu Pro Asp Asp Phe Ser Lys Lys Phe Asn Ile Met Phe 165 170 175 Ser Lys Pro Phe Phe Leu Glu Phe Thr Asp Ile Asn Val Ser Lys Gly 180 185 190 Asn Ala Leu Lys Phe Leu Thr Glu Tyr Tyr Gly Leu Lys Arg Glu Glu 195 200 205 Val Met Ala Ile Gly Asp Gly Asp Asn Asp Ile Ser Met Ile Glu Tyr 210 215 220 Ala Gly Ile Gly Val Ala Val Glu Asn Ala Val Glu Lys Leu Lys Glu 225 230 235 240 Ala Ala Asp Phe Val Val Ala Lys Ser Asp Asp Ser Gly Phe Ala Gln 245 250 255 Ala Ile Glu Lys Val Phe Asn Val His Phe 260 265 <210> 23 <211> 279 <212> PRT <213> Caldicellulosiruptor bescii <400> 23 Met Tyr Lys Leu Ile Ala Ile Asp Leu Asp Met Thr Leu Leu Asp Lys 1 5 10 15 Asn Lys Asn Ile Ser Ser Arg Asn Lys Arg Ala Ile Glu Leu Val Lys 20 25 30 Gln Lys Gly Val Gln Ile Val Leu Cys Ser Gly Arg Ile Leu Lys Gly 35 40 45 Val Met Tyr Phe Ala Lys Val Leu Gly Leu Tyr Asp Gln Val Ile Val 50 55 60 Ala Cys Asn Gly Ala Ile Val Arg Asp Leu Lys Lys Asn Asn Asp Ile 65 70 75 80 Tyr Tyr Ile Gly Leu Glu Asn Ser Lys Ser Leu Glu Ile Ala Arg Ile 85 90 95 Cys Lys Glu Asn Asp Ile Tyr Tyr His Tyr Tyr Phe Gln Asp Thr Met 100 105 110 Ile Ala Arg Arg Leu Asp Tyr Ser Ser Lys Phe Tyr Tyr Glu Lys Asn 115 120 125 Lys Glu Leu Pro Glu Glu Glu Arg Ile Asn Ile Ile Ile Asp Asp Ser 130 135 140 Glu Asn Thr Ile Asn Ala Cys Gly Asp Leu Ile Thr Lys Phe Val Ile 145 150 155 160 Ile Asp Lys Asp Leu Glu Lys Val Asn Tyr Val Arg Lys Ile Ile Glu 165 170 175 Arg Glu Ile Pro Gly Val Glu Thr Thr Lys Ser Asp Ile Asn Ile Leu 180 185 190 Glu Val Met Lys Glu Gly Val Asn Lys Lys Arg Ala Leu Glu Phe Val 195 200 205 Ile Ser Tyr Leu Gly Ile Ala Pro Glu Glu Val Met Ala Ile Gly Asp 210 215 220 Asn Glu Asn Asp Leu Glu Met Ile Glu Phe Ala Gly Leu Gly Val Ala 225 230 235 240 Met Gly Asn Ala Ile Glu Glu Leu Lys Lys Ile Ala Asp Tyr Val Thr 245 250 255 Ser Ser Tyr Glu Asn Asp Gly Val Ala Arg Ala Ile Glu Lys Phe Val 260 265 270 Leu Gly Glu Ala Val Asn Val 275 <210> 24 <211> 284 <212> PRT <213> Caldicellulosiruptor bescii <400> 24 Met Ile Lys Leu Ile Ala Leu Asp Ile Asp Gly Thr Leu Leu Asp Asp 1 5 10 15 Arg Gly Tyr Ile Pro Gln Ile Asn Arg Glu Phe Leu Lys Ile Ala Val 20 25 30 Glu Lys Tyr Lys Ile Val Ile Val Leu Cys Thr Gly Arg Gly Ala Ser 35 40 45 Ala Phe Lys Ile Ala Lys Asp Leu Gln Leu Pro Cys Ser Leu Ile Ser 50 55 60 Ala Asn Gly Val Tyr Val Phe Glu Asn Pro Asn Phe Pro Pro Ile Ile 65 70 75 80 Lys Asn Tyr Leu Thr Glu Arg Gln Lys Lys Val Leu Ile Glu Phe Leu 85 90 95 Asp Asn Asn His Phe Glu Ile Asp Tyr Tyr Ile Val Leu Gly Tyr Glu 100 105 110 Gln Asp Phe His Met Ile Tyr Lys Glu Arg Thr Asn Tyr Asp Ser Tyr 115 120 125 Phe Leu Ser Phe Val Asn Gly Arg Lys Gln Phe Lys Ser Thr Tyr Pro 130 135 140 Ala Glu Lys Leu Leu Lys Phe Leu Glu Tyr Pro Ile Ser His Thr Gly 145 150 155 160 Ile Val Gly Lys Tyr Glu Lys Leu Lys Glu Ile Lys Glu Ile Leu Lys 165 170 175 Thr Leu Glu Leu Asp Cys Asn Ile Ile Leu Tyr Tyr Ala Ser Asp Asn 180 185 190 Lys Glu Tyr Gly Phe Leu Glu Val Leu Ser Asn Asn Ala Ser Lys Glu 195 200 205 Lys Ala Leu Leu Gln Phe Met Asn Phe Lys Asn Ile Ser Ser Glu Glu 210 215 220 Leu Ile Ser Ile Gly Asp Asn Phe Asn Asp Val Gly Met Phe Lys Ile 225 230 235 240 Ser Gly Ile Ser Val Ala Val Ala Asn Ala Pro Glu Glu Val Lys Lys 245 250 255 Ala Ala Lys Phe Val Thr Ser Arg Thr Asn Asn Glu Gly Ala Val Ala 260 265 270 Glu Ala Ile Glu Met Phe Ile Ile Lys Arg Asp Gly 275 280 <210> 25 <211> 286 <212> PRT <213> Caldilinea aerophila <400> 25 Met Arg Pro Phe Asp Leu Val Val Leu Asp Leu Asp Gly Thr Ile Leu 1 5 10 15 Asn Ala Tyr Gln Arg Thr Arg Ile Ser Lys Ala Val His Asp Ala Ile 20 25 30 Glu Ala Val Gln Ala Ala Gly Val Pro Val Thr Ile Gly Thr Gly Arg 35 40 45 Thr Leu Asp Tyr Ile Arg Tyr His Leu Pro Gly Asp Leu His Leu Thr 50 55 60 Tyr Pro Val Ile Ala Thr Gln Gly Ala Val Ile Gly Asp Pro Val Ser 65 70 75 80 Gly Arg Val Leu Val Glu Ile Pro Leu Pro Leu Lys Glu Ala Arg Ala 85 90 95 Ile Ala Ala Phe Val Asp Asp His Arg Tyr Thr Thr Ala Phe Tyr Phe 100 105 110 Asn Asn Ala Asp Gly His Thr Val Ile Tyr Gln Asn Ala Ala Gly Ala 115 120 125 Thr Ala Glu Glu Glu Ala Leu Leu His His Leu Leu Gly Val Pro Asn 130 135 140 Ala Leu Val Glu Gln Phe Ser Pro Leu Leu Ala Asn Glu Asp Ala His 145 150 155 160 Pro Pro Ile Lys Val Ile Ser Phe Asn His Gly Leu Pro Asp Ala Val 165 170 175 Asp Leu Leu His Glu Tyr Gln Arg Arg Phe Ser Pro Pro Leu Thr Val 180 185 190 Thr Arg Thr His Glu Trp Leu Val Glu Ala Thr Ala Pro Gly Val Asp 195 200 205 Lys Gly Ser Gly Leu Leu Arg Leu Cys Lys Leu Leu Gly Val Asp Pro 210 215 220 Gln Arg Val Leu Ala Ile Gly Asp Ser Asp Asn Asp Ile Pro Met Leu 225 230 235 240 Glu Val Ala Gly Phe Ala Ile Ala Met Gly Asn Ala Asn Glu Arg Val 245 250 255 Lys Ala Val Ala Asp Trp Val Ala Pro Ser Ile Asp Glu Glu Gly Ala 260 265 270 Ala Val Ala Leu Arg Arg Trp Val Leu Glu Pro Met Ser Ala 275 280 285 <210> 26 <211> 256 <212> PRT <213> Caldilinea aerophila <400> 26 Met Lys Asp Lys Thr Lys Val Leu Ala Leu Asp Leu Asp Asp Thr Leu 1 5 10 15 Leu Arg Ser Asp Lys Ser Ile Gly Ala Ala Thr Leu Ala Ala Leu Asp 20 25 30 Arg Trp Leu Ala Ala Gly His Glu Ile Val Val Ala Thr Gly Arg Pro 35 40 45 Pro Arg Asn Val Ala Pro Val Leu Pro Pro Ser Leu Leu His Val Pro 50 55 60 Arg Ile Val Tyr Asn Gly Ala Gln Ala Ile Leu Gly Asp Glu Ile Ile 65 70 75 80 Phe Asp Arg Pro Ile Pro Ala Gln Asp Val Arg His Ile Leu Glu Trp 85 90 95 Ser Lys Arg Ser Gly Glu Ala Trp Tyr Ile Gly Leu Glu Ile Asn Asp 100 105 110 Met Leu Tyr Val Asn Arg His Phe Glu Lys Pro Gly Thr Phe Gln Val 115 120 125 Val Asp Leu Met Gln Leu Cys Asp Gln Pro Val Tyr Lys Ile Ile Phe 130 135 140 Phe Phe Pro Asp Gly Arg Arg Asn Ile Glu Pro Leu Leu Ala Ala Met 145 150 155 160 Pro Pro Thr Thr Arg Ala Leu Val Thr Pro Lys Phe Ser Leu Val Gln 165 170 175 Leu Cys Ala Arg Asp Ala Asn Lys Phe Thr Ala Leu Lys Gln Leu Leu 180 185 190 Ala Arg Arg Pro Leu Ala Pro Ala Ala Val Ile Ala Val Gly Asp Asp 195 200 205 Ile Asn Asp Ile Glu Met Val Arg Cys Ser Asp Ile Gly Val Ala Val 210 215 220 Ala Asn Ala Leu Pro Glu Val Lys Ala Val Ala Asp Trp Ile Ala Pro 225 230 235 240 Gly Ala Asp Asp Asp Gly Val Ala Tyr Val Ile Asp Arg Leu Leu Asn 245 250 255 <210> 27 <211> 223 <212> PRT <213> Caldilinea aerophila <400> 27 Met Thr Ile Arg Ala Ile Leu Trp Asp Leu Asp Gly Thr Leu Ala Asp 1 5 10 15 Thr Thr Ala Leu His Tyr Gln Ala Trp Arg Lys Thr Met Gln Arg Tyr 20 25 30 Gly Val Asp Leu Thr Tyr Glu Ser Phe Ile Lys Asp Tyr Gly Arg Asn 35 40 45 Asn Ala Glu Ile Leu Ala Glu His Phe Gly Ile His Ala Val Ala Thr 50 55 60 Ile Gln Gln Val Ala Asp Glu Lys Glu Ser Ala Phe Arg Ser Leu Ile 65 70 75 80 Thr Pro Gly Val Leu Gln Pro Leu Pro Gly Ala Leu Ala Trp Leu His 85 90 95 Cys Phe Arg Ser Leu Gly Met Ile Gln Val Ile Gly Ser Ser Gly Pro 100 105 110 Met Ala Asn Ile Ala Ala Val Val His Ala Leu Gly Val Gly Asp Phe 115 120 125 Phe Phe Gly Leu Val Ser Gly Val His Leu Pro Glu Gly Lys Pro Glu 130 135 140 Pro Thr Ile Phe Leu Arg Cys Ala Ala Leu Ala Gly Ala Ala Pro Asp 145 150 155 160 Glu Cys Leu Val Ile Glu Asp Ser Leu His Gly Ile Glu Ala Ala Met 165 170 175 Arg Ala Gly Met Thr Ser Val Ala Val Gly Arg Ile Ala Ala Gln Pro 180 185 190 Ala Leu Gln Asp Leu Ile Gln Lys Asn Pro Gly Leu Arg Cys Ile Val 195 200 205 Thr Pro Ser Leu Ala Ser Ile Asp Asp Pro Lys Ser Ile Leu Thr 210 215 220 <210> 28 <211> 275 <212> PRT <213> Caldithrix abyssi <400> 28 Met Ser Glu Val Met Lys Lys Met Leu Val Leu Asp Phe Asp Gly Thr 1 5 10 15 Leu Phe Gln Pro Gly Lys Phe Ile Ser Asp Gln Asp Leu Lys Leu Leu 20 25 30 Lys Ala Leu Ala Asn Met Asn Val Val Arg Val Ile Ala Thr Gly Arg 35 40 45 Ser Leu Phe Ser Leu Lys Lys Val Ile Asp Met Ser Phe Pro Ile Asp 50 55 60 Tyr Leu Ile Leu Ser Thr Gly Val Gly Ile Leu Asp Trp Arg Ser Gln 65 70 75 80 Lys Leu Ile Arg Ser Tyr Asp Met Glu Gly Gly Leu Val Asp Glu Ile 85 90 95 Ile Ala Ile Leu Lys Arg Arg Arg His Ser Phe Phe Val His Gln Pro 100 105 110 Ala Pro Gln Asn His Lys Phe Phe Tyr Tyr Asp Gly Ile Ala Pro Arg 115 120 125 Gln Asp Phe Thr Arg Arg Phe Glu Leu Tyr Arg Asp Phe Ala Arg Pro 130 135 140 Leu Lys Gln Arg Lys Arg Gly Met Ala Ala Gly Gln Ile Leu Val Ile 145 150 155 160 Ser Glu Glu Leu Gln Gln Ile Lys Glu Glu Leu Gln Pro Leu Met His 165 170 175 Arg Ile Asn Ile Ile Arg Thr Thr Ser Pro Leu Asp Gly Arg Ser Met 180 185 190 Trp Val Glu Ile Phe Asp Lys Arg Val Asn Lys Ala Leu Ala Ala Gln 195 200 205 Phe Leu Ala Glu Arg Leu Asn Ile Ser Val Arg Asn Ile Val Ala Leu 210 215 220 Gly Asn Asp Tyr Asn Asp Leu Asp Leu Leu Asn Trp Ala Gly Ser Ala 225 230 235 240 Phe Val Val His Thr Ala Pro Glu Glu Leu Lys Glu Lys Phe Gln Ile 245 250 255 Ile Lys Asn Ala Asp Asp Gly Leu Leu Ile His Ile Met Pro Leu Leu 260 265 270 Gln Arg Gly 275 <210> 29 <211> 171 <212> PRT <213> Caldithrix abyssi <400> 29 Met Arg Lys Lys Leu Ser Gln Ile Gln Leu Ile Leu Met Asp Val Asp 1 5 10 15 Gly Val Leu Thr Ala Gly Glu Ile Ile Tyr Ser Ala Ala Gly Asp Glu 20 25 30 Leu Lys Met Phe Asn Val Gln Asp Gly Met Gly Ile Thr Leu Ala Arg 35 40 45 Met Ala Gly Leu Lys Thr Gly Ile Leu Thr Gly Arg Lys Ser Glu Leu 50 55 60 Val Arg Arg Arg Ala Glu Glu Leu Lys Ile Asp Ile Val Ser Gln Gly 65 70 75 80 Ser Phe Glu Lys Leu Pro Glu Tyr Glu Lys Ile Lys Gln Gln Met Gln 85 90 95 Leu Ser Asp Glu Gln Ile Cys Tyr Ile Gly Asp Asp Val Leu Asp Ile 100 105 110 Pro Val Leu Lys Arg Val Gly Phe Ser Val Ala Val Ala Asn Ala Arg 115 120 125 Asp Glu Val Lys Ala Ile Ala Asp Tyr Val Thr Val Ala Glu Gly Gly 130 135 140 Lys Gly Ala Val Arg Glu Val Ile Asp Lys Ile Leu Lys Trp Gln Asn 145 150 155 160 Lys Leu His Ala Leu Ile Asp Gln Leu Ser Arg 165 170 <210> 30 <211> 354 <212> PRT <213> Caldithrix abyssi <400> 30 Met Lys Arg Gly His Arg Tyr Ile Val Phe Thr Met Ala Ile Phe Ser 1 5 10 15 Leu Ile Leu Ile Ala Cys Thr Lys Lys Glu Val Lys Pro Asp Leu Ala 20 25 30 Phe Ala Gly Glu Lys His Leu Lys Asn Ile Arg Met Leu Thr Asn Gly 35 40 45 Gly Glu Asn Ala Glu Ala Tyr Phe Ser Phe Asp Glu Ser Gln Leu Ile 50 55 60 Tyr Gln Ser Lys His Asp Ser Ile Lys Cys Asp Gln Ile Phe Ile Met 65 70 75 80 Asn Leu Asp Gly Ser Asp Lys Arg Met Val Ser Thr Gly Lys Gly Arg 85 90 95 Thr Thr Cys Ser Tyr Phe Leu Pro Gly Asp Gln Gln Ile Ile Tyr Ala 100 105 110 Ser Thr His Gly Val Asp Glu Asn Cys Pro Pro Pro Pro Asp Phe Ser 115 120 125 Arg Gly Tyr Val Trp Lys Val Tyr Ser Ser Tyr Asp Leu Tyr Ile Ala 130 135 140 Asn Ala Asp Gly Ser Asp Pro Gln Pro Phe Leu Pro Ser Pro Gly Tyr 145 150 155 160 Asp Ala Glu Ala Thr Val Ser Pro Arg Gly Asp Lys Ile Val Phe Thr 165 170 175 Ser Gln Arg Asn Gly Asp Leu Asp Ile Tyr Thr Val Asn Ile Asp Gly 180 185 190 Ser Gly Leu Lys Gln Leu Thr His Glu Ile Gly Tyr Asp Gly Gly Ala 195 200 205 Phe Phe Ser Trp Asp Gly Ser Lys Ile Val Tyr Arg Ala Tyr His Pro 210 215 220 Arg Thr Glu Glu Glu Leu Lys Arg Tyr Arg Lys Leu Leu Ala Glu Glu 225 230 235 240 Leu Ile Glu Pro Asn Asn Phe Gln Leu Phe Val Met Asp Ala Asp Gly 245 250 255 Ser Asn Lys Arg Gln Ile Thr His Asn Asp Phe Ala Asn Phe Ala Pro 260 265 270 Phe Phe His Pro Asp Asn Lys Arg Ile Ile Phe Cys Ser Asn Met Gly 275 280 285 Thr Thr Glu Ser Thr Gln Arg Asp Phe Asn Leu Trp Met Ile Asn Glu 290 295 300 Asp Gly Thr Gly Leu Lys Gln Ile Thr Phe Phe Lys Gly Phe Asp Gly 305 310 315 320 Phe Pro Met Phe Thr His Asp Gly Lys Lys Leu Val Phe Ala Ser Asn 325 330 335 Arg Phe Asn Lys Lys Lys Gly Glu Thr Asn Val Phe Ile Ala Asp Trp 340 345 350 Val Glu <210> 31 <211> 222 <212> PRT <213> Caldithrix abyssi <400> 31 Met Arg Asn Lys Ala Val Leu Phe Asp Phe Asp Gly Val Val Val Lys 1 5 10 15 Ser Met Glu Gln His Phe Asn Ala Trp Arg Gln Ala Phe Leu Glu Lys 20 25 30 Gly Val Glu Ile Lys Glu Asp Glu Phe Phe Val Leu Glu Gly Gln Gly 35 40 45 Ile Asn Thr Ile Ala His His Leu Gly Lys Ile Tyr Gly Leu Asn Arg 50 55 60 Gln Gln Val Glu Glu Val Met Glu Arg Lys Val Asn Tyr Tyr Asn Gln 65 70 75 80 Phe Met Thr Leu Glu Phe Tyr Asp His Phe His Glu Leu Val Glu His 85 90 95 Leu His Arg Arg Gln Val Pro Met Gly Val Val Thr Gly Gly Asn Arg 100 105 110 Ser Arg Val Glu Lys Ile Ile Asn Glu His Phe Asn His Tyr Phe Arg 115 120 125 Ala Leu Val Thr Val Asp Asp Val Glu Arg Gly Lys Pro Phe Pro Asp 130 135 140 Pro Phe Leu Lys Ala Ala Gln Met Leu Asn Met Ala Pro Gln Asn Cys 145 150 155 160 Ile Val Val Glu Asn Ala Pro Met Gly Ile Lys Gly Ala Lys Arg Ala 165 170 175 Gly Met Thr Val Val Ala Ile Thr Thr Thr Leu Lys Pro Asp Tyr Leu 180 185 190 Lys Gln Ala Asp Tyr Ile Ala His Asn Phe Leu Glu Val Glu Glu Ile 195 200 205 Leu Asn Thr Leu Leu Gly Ile Glu Arg Thr Glu Thr Ile Lys 210 215 220 <210> 32 <211> 268 <212> PRT <213> Carboxydocella sp. ULO1 <400> 32 Met Val Leu Val Lys Leu Ile Ala Ile Asp Leu Asp Asp Thr Leu Leu 1 5 10 15 Arg Thr Asp Cys Thr Ile Ser Pro Arg Ala Gln Ala Ala Ile Lys Ala 20 25 30 Ala Ser Ala Arg Gly Val Ala Val Thr Leu Ala Thr Gly Arg Met Tyr 35 40 45 Arg Ser Ala Arg Pro Tyr Ala Leu Glu Leu Gly Leu Asp Leu Pro Leu 50 55 60 Ile Thr Tyr Gln Gly Ala Leu Ile Lys Ser Gly Leu Ser Gly Glu Glu 65 70 75 80 Leu Trp His Cys Pro Leu Pro Pro Glu Met Ala Ala Glu Ile Ile Thr 85 90 95 Leu Ala Arg Glu Glu Gly Ile His Ala Asn Ile Tyr Leu Asp Asp His 100 105 110 Leu Tyr Val Glu Lys Ala Thr Ala Glu Ala Glu Ala Tyr Ser Gln Leu 115 120 125 Ala Arg Val Pro Tyr Thr Val Glu Glu Asp Leu Arg Gly Arg Ile Leu 130 135 140 Ala Ser Gly Gln Gly Pro Ser Lys Ile Leu Leu Ile Ala Glu Pro Glu 145 150 155 160 Arg Leu Asp Gln Leu Ala Ala Ala Leu Arg Pro Arg Trp Gln Gly Lys 165 170 175 Val Gln Leu Ala Lys Ser Lys Asp His Tyr Leu Glu Phe Thr His Pro 180 185 190 Glu Ala Ser Lys Gly Gln Ala Leu Ile Ala Leu Cys Arg Tyr Leu Asp 195 200 205 Leu Glu Leu Lys Asp Ala Met Ala Ile Gly Asp Ser Phe Asn Asp Leu 210 215 220 Asp Met Ile Glu Leu Ala Gly Ile Gly Val Ala Met Gly Asn Ala Lys 225 230 235 240 Pro Glu Val Lys Ala Ile Ala Asp Tyr Val Thr Leu Ala Asn Asp Asp 245 250 255 Asp Gly Val Ala Glu Ala Ile His Arg Phe Val Leu 260 265 <210> 33 <211> 266 <212> PRT <213> Carboxydothermus ferrireducens <400> 33 Met Arg Tyr Arg Leu Leu Ala Val Asp Leu Asp Asp Thr Phe Leu Asn 1 5 10 15 Lys Glu Leu Gln Val Ser Pro Arg Val Gln Gln Ala Val Ile Glu Ala 20 25 30 Leu Lys Lys Gly Ile Ile Val Thr Leu Ala Thr Gly Arg Met Tyr Arg 35 40 45 Ser Ala Lys Lys Tyr Ala Phe Ser Phe Leu Gly Asp Ile Pro Leu Ile 50 55 60 Thr Tyr Asn Gly Ala Leu Ile Lys Tyr Ser Arg Ser Glu Arg Glu Ile 65 70 75 80 Tyr His Gln Pro Val Pro Gly Glu Leu Ala Leu Thr Ile Tyr Arg Arg 85 90 95 Val Lys Gly His Phe His Leu Asn Val Tyr Gln Asp Asp Glu Leu Phe 100 105 110 Val Glu Glu Asp Asn Gln Tyr Ile Arg Asp Tyr Ser Lys Ile Ala Gly 115 120 125 Val Pro Phe Arg Val Val Asn Asn Ile Glu Glu Leu Leu Gly Lys Lys 130 135 140 Ala Pro Thr Lys Leu Leu Ala Ile Gly Asp Pro Glu Glu Leu Asp Gln 145 150 155 160 Leu Trp Glu Glu Thr Asn Gly Glu Phe Arg Gly Val Leu His Ile Thr 165 170 175 Lys Ser Lys Pro His Tyr Leu Glu Phe Leu Ala Ala Gly Val Asn Lys 180 185 190 Gly Glu Ala Leu Lys Ile Leu Ala Gln His Leu Arg Ile Ser Leu Lys 195 200 205 Glu Thr Val Ala Val Gly Asp Ser Tyr Asn Asp Leu Glu Met Leu Glu 210 215 220 Ala Ala Gly Leu Gly Val Ala Met Gly Asn Ala Leu Pro Glu Val Lys 225 230 235 240 Arg Arg Ala Asp Leu Val Val Pro Ala Asn Asp Glu Asp Gly Ile Ala 245 250 255 Tyr Leu Ile Asn Glu Ile Ile Leu Lys Asn 260 265 <210> 34 <211> 231 <212> PRT <213> Carboxydothermus ferrireducens <400> 34 Met Ala Tyr Ile Leu Phe Asp Leu Asp Gly Thr Leu Leu Pro Leu Asn 1 5 10 15 Leu Asp Tyr Phe Leu Lys Leu Tyr Ile Gln Glu Val Lys Ser Tyr Val 20 25 30 Lys Gly Lys Phe Asp Pro Asp Leu Ile Val Lys Ala Ile Leu Lys Ala 35 40 45 Thr Glu Glu Met Val Lys Asn Thr Gly Glu Lys Leu Asn Ser Glu Val 50 55 60 Phe Trp Gln Ala Phe Asp Arg Val Tyr Pro Gly Thr Ala Asn Leu His 65 70 75 80 Asn Val Phe Glu Asp Phe Tyr His Thr Ser Phe Lys Asn Ile Gly Asn 85 90 95 Tyr Phe Lys Pro His Pro Leu Ala Arg Pro Met Ile Arg Tyr Leu His 100 105 110 Lys Lys Gly His Thr Leu Ile Leu Ala Thr Asn Ala Leu Phe Pro Arg 115 120 125 Ser Ala Ile Ile Glu Arg Leu Tyr Trp Ala Asn Leu Ser Pro Lys Tyr 130 135 140 Phe Arg Phe Ile Thr His Tyr Asp Asn Met His Tyr Cys Lys Pro Asn 145 150 155 160 Pro Asn Tyr Tyr Lys Glu Ile Leu Glu Lys Ile Lys Ala Arg Pro Ser 165 170 175 Asp Cys Leu Met Ile Gly Asn Asp Pro Ala Glu Asp Leu Val Ala Lys 180 185 190 Glu Leu Gly Ile Lys Thr Phe Leu Val Lys Asp Tyr Glu Val Lys Arg 195 200 205 Glu Asn Pro Arg Thr Pro Asp Tyr Ala Gly Asp Phe Tyr Glu Leu Tyr 210 215 220 Arg Phe Ile Arg Glu Asn Phe 225 230 <210> 35 <211> 212 <212> PRT <213> Carboxydothermus ferrireducens <400> 35 Met Ile Lys Ala Val Phe Phe Asp Leu Asp Gly Thr Leu Leu Asp Thr 1 5 10 15 Phe Asp Leu Ile Tyr Glu Ser Phe Lys His Val Tyr Lys Asn Phe Leu 20 25 30 Asn Lys Asp Ile Thr Arg Glu Glu Ile Tyr Pro Tyr Phe Gly Lys Pro 35 40 45 Leu Ile Tyr Ser Phe Glu Asn Leu Asp Pro Glu Thr Ile Asp Gln Val 50 55 60 Ile Ala Ala Tyr Arg Glu Phe Asn Leu Gln His His Asp Gln Met Val 65 70 75 80 Lys Pro Phe Pro Gly Ala Lys Glu Thr Leu Glu Lys Leu Lys Gln Arg 85 90 95 Gly Lys Ile Leu Ala Val Ile Thr Ser Lys Val Lys Ser Thr Ala Ile 100 105 110 Arg Gly Leu Lys Leu Phe Asn Leu Asp Arg Tyr Phe Asp Leu Val Val 115 120 125 Ala Leu Glu Asp Thr Glu Lys His Lys Pro Asp Pro Ala Pro Val Leu 130 135 140 Tyr Ala Leu Lys Phe Phe Gln Val Lys Pro Glu Gln Cys Leu Met Val 145 150 155 160 Gly Asp Ser Pro His Asp Met Val Ser Ala Gln Arg Ala Gly Val Lys 165 170 175 Thr Ala Ala Val Lys Trp Ser Val Leu Pro Trp Glu Asp Leu Val Lys 180 185 190 Thr Lys Pro Asn Tyr Ile Leu Asn Ser Phe Asp Asp Leu Leu Lys Ile 195 200 205 Thr Gly Val Glu 210 <210> 36 <211> 218 <212> PRT <213> Chloroflexi bacterium 54-19 <400> 36 Met Asn Lys Phe Gln Gly Val Ile Leu Asp Ile Asp Gly Thr Leu Ile 1 5 10 15 Asp Ser Asn Asp Ala His Ala His Ser Trp Val Asp Thr Phe Ala Glu 20 25 30 Ala Gly Ile Pro Val Pro Phe Glu Arg Val Arg Pro Leu Ile Gly Glu 35 40 45 Gly Gly Asp Lys Leu Leu Pro Glu Val Ser Gly Phe Glu Ala Gly Ser 50 55 60 Ala Glu Gly Lys Arg Leu Thr Lys Arg Arg Thr Glu Ile Phe Lys Glu 65 70 75 80 Lys Tyr Leu Pro His Ile Gln Pro Phe Pro Lys Thr Arg Glu Leu Ile 85 90 95 Thr Ala Phe Glu Lys Arg Gly Leu Lys Leu Gln Ile Ala Thr Ser Ala 100 105 110 Gln Glu Ala Glu Val Lys Asp Leu Leu Lys Val Ala Gly Thr Pro Glu 115 120 125 Ile Ile Glu Gln Lys Thr Thr Ser Asp Asp Ala Glu Lys Ser Lys Pro 130 135 140 Asp Pro Asp Ile Ile His Ala Ala Leu Gln Lys Ile Gly Leu Glu Ala 145 150 155 160 Gly Gln Val Val Met Leu Gly Asp Thr Pro Tyr Asp Val Ala Ala Ala 165 170 175 Lys Lys Ala Gly Val Lys Ser Tyr Ala Phe Arg Cys Gly Gly Trp Trp 180 185 190 Gln Asp Arg Asp Phe Glu Asp Ala Ala Ala Ile Phe Asp Gly Pro Ala 195 200 205 Asp Leu Leu Glu Lys Leu Asp Asp Tyr Leu 210 215 <210> 37 <211> 284 <212> PRT <213> Defluviitoga tunisiensis <400> 37 Met Asn Tyr His Leu Asn Ser Ser Thr Lys Val Phe Val Phe Asp Phe 1 5 10 15 Asp Gly Thr Leu Leu Asn Ser Glu Val Arg Ile Ser Pro Arg Thr Phe 20 25 30 Glu Ala Leu Lys Lys Leu Lys Glu His Gly His Thr Ile Ile Leu Cys 35 40 45 Ser Gly Arg Met Tyr Ala Ser Met Leu Phe Ile Val Glu Asn Phe Leu 50 55 60 Pro Phe Leu Lys Gly Tyr Ala His Ile Val Ser Tyr Asn Gly Gly Tyr 65 70 75 80 Ile Val Asn Asn Lys Gly Glu Leu Leu Phe Glu Glu Gly Leu Asp Lys 85 90 95 Asp Val Ala Ile Asp Cys Ile Glu Phe Leu Arg Glu Leu Asn Val His 100 105 110 Arg His Val Tyr Ile Asn Asp Glu Leu Ile Ser Glu Gln Asp Asp Gln 115 120 125 Glu Ile Lys Asp Tyr Ser Lys His Ser Phe Val Ala Tyr Lys Leu Val 130 135 140 Asp Asp Leu Val Asp Thr Ile Lys Asn Ser Glu His Pro Thr Leu Lys 145 150 155 160 Ile Leu Gly Ile Cys Glu Gln Glu Lys Leu Asn Met Val Gln Ser Leu 165 170 175 Ala Glu Lys Lys Phe Glu Gly Lys Ile Asn Ile Met Arg Ser Phe Ser 180 185 190 Thr Tyr Leu Asp Phe Met Pro Phe Gly Val Ser Lys Gly Glu Ala Leu 195 200 205 Lys Ile Leu Ser Lys Ile Tyr Asn Phe Asn Val Glu Glu Val Tyr Val 210 215 220 Phe Gly Asp Ser Gln Asn Asp Ile Asp Met Leu Arg Ile Ser Arg Asn 225 230 235 240 Ser Phe Ala Met Gly Asn Ala Gln Lys Asp Val Lys Glu Val Ala Arg 245 250 255 Tyr Val Ile Pro Ser Asn Asp Glu Asp Gly Val Ala Phe Ala Ile Glu 260 265 270 Lys Ile Leu Ser Asp Asp Leu Asp Asn Val Asn Ile 275 280 <210> 38 <211> 231 <212> PRT <213> Deinococcus aerius <400> 38 Met Thr Val Pro Gln Pro Thr Asp Pro Ala Thr Val Leu Asn Pro Glu 1 5 10 15 Arg Leu Arg Gly Val Leu Leu Asp Val Asp Gly Thr Leu Ile Asp Ser 20 25 30 Asn Asp Ala His Ala Arg Ala Trp Val Glu Ala Leu Arg Glu Glu Gly 35 40 45 Phe Glu Arg Ser Phe Gly Glu Val Arg Pro Leu Ile Gly Met Gly Gly 50 55 60 Asp Gln Leu Val Pro Arg Leu Thr Gly Glu Asp Gly Glu Ser Glu Val 65 70 75 80 Gly Lys Arg Leu Thr Gln Gly Trp Leu Lys His Phe Lys Pro Leu Val 85 90 95 Pro Gly Leu Arg Pro Thr Arg Gly Asn Arg Ala Leu Ile Glu Gly Leu 100 105 110 Arg Ser Arg Gly Leu Lys Val Val Leu Ala Thr Ser Gly Glu Ala Glu 115 120 125 Ile Val Asp Ser Leu Leu Lys Gln Ala Asn Leu Gly Asp Leu Asn Leu 130 135 140 Asp Arg Val Ser Ser Ser Glu Val Gly Ser Ser Lys Pro Ala Pro Asp 145 150 155 160 Leu Val Gln Val Gly Leu Asp Lys Leu Gly Leu Pro Ala Gly Ala Ala 165 170 175 Leu Met Val Gly Asp Thr Pro Phe Asp Ala Glu Ala Ala His Gly Ala 180 185 190 Gly Val Pro Cys Ala Leu Leu Arg Cys Gly Gly Asp Ser Glu Glu Glu 195 200 205 Leu Gly Arg Thr Gly Ala Leu Val Leu Asn Asp Pro Gln Ala Leu Leu 210 215 220 Glu Ala Leu Glu Gly Ala Ser 225 230 <210> 39 <211> 231 <212> PRT <213> Deinococcus apachensis <400> 39 Met Thr Ala Leu Gln Pro Ala Asp Pro Ala Ala Ala Leu Asn Pro Asp 1 5 10 15 Arg Leu Arg Gly Val Leu Leu Asp Val Asp Gly Thr Leu Ile Asp Ser 20 25 30 Asn Asp Ala His Ala Arg Ala Trp Val Glu Ala Leu Arg Glu Glu Gly 35 40 45 Phe Glu Arg Ser Phe Gly Glu Val Arg Pro Leu Ile Gly Met Gly Gly 50 55 60 Asp Gln Leu Ile Pro Arg Leu Thr Gly Glu Asp Gly Glu Ser Glu Leu 65 70 75 80 Gly Lys Arg Leu Thr Gln Gly Trp Leu Lys His Phe Lys Pro Leu Ile 85 90 95 Pro Glu Leu Gln Pro Thr Arg Gly Asn Arg Ala Leu Ile Glu Gly Leu 100 105 110 Arg Ala Arg Gly Leu Arg Val Ala Leu Ala Thr Ser Gly Glu Ala Glu 115 120 125 Ile Val Asp Gly Leu Leu Lys Gln Ala Gly Leu Asp Asp Leu Asn Leu 130 135 140 Asp Arg Val Ser Ser Ser Glu Val Glu Ser Ser Lys Pro Glu Pro Asp 145 150 155 160 Leu Val Glu Ala Gly Leu Asn Lys Leu Gly Leu Pro Ala Asp Ala Ala 165 170 175 Leu Met Val Gly Asp Thr Pro Phe Asp Ala Glu Ala Ala His Lys Ala 180 185 190 Gly Val Pro Cys Val Leu Leu Arg Cys Gly Gly Asp Ser Asp Glu Glu 195 200 205 Leu Gly Arg Ala Pro Ala Leu Val Leu Asp Asp Pro Gln Ala Leu Leu 210 215 220 Glu Ala Leu Glu Gly Thr Ser 225 230 <210> 40 <211> 228 <212> PRT <213> Deinococcus aquatilis <400> 40 Met Arg Arg Ala Glu Asp Val Tyr Leu Asp Arg Met Tyr Lys Gly Val 1 5 10 15 Ile Phe Asp Ile Asp Gly Thr Leu Val Asp Ser Asn Asp Ala His Ala 20 25 30 Arg Ala Trp Val Lys Ala Phe Ala Asp Glu His Ile Thr Val Pro Phe 35 40 45 Asp Gln Val Arg Pro Leu Ile Gly Met Gly Ser Asp Gln Met Val Pro 50 55 60 Arg Leu Thr Asp Val Gln Lys Asp Ser Pro Thr Phe Lys Arg Leu Gly 65 70 75 80 Glu Ala Trp Lys Gln His Phe Gln Ala Glu Glu Met Pro His Leu Lys 85 90 95 Ala Gln Pro Gly Val Arg Ala Leu Ile Glu Arg Leu Gln Glu Arg Gly 100 105 110 Leu Arg Leu Ile Val Gly Thr Ser Ala Asp Glu Ala Leu Val Ala Asp 115 120 125 Leu Leu Arg Val Ala Gly Val Asp Asp Leu Leu Thr Glu Tyr Thr Thr 130 135 140 Ala Ser Asp Val Glu Ser Ser Lys Pro Glu Pro Asp Ile Val Gln Ala 145 150 155 160 Ala Val Thr Lys Leu Gly Leu Ala Pro Ala Glu Val Leu Met Val Gly 165 170 175 Asp Thr Pro Phe Asp Ile Glu Ser Ala His Lys Ala Gly Val Ala Thr 180 185 190 Val Ala Leu Arg Cys Gly Gly Asp Thr Arg Phe Glu Gly Ala Ala Ala 195 200 205 Val Tyr Ala Asp Pro Gln Glu Trp Leu Glu Gln Leu Asp Gln Ser Pro 210 215 220 Leu Gly Lys Gly 225 <210> 41 <211> 229 <212> PRT <213> Deinococcus geothermalis <400> 41 Met Ser His Pro Glu Pro Thr Ala Pro Ala Ala Ala Leu Asp Pro Lys 1 5 10 15 Arg Leu Arg Gly Val Leu Leu Asp Val Asp Gly Thr Leu Ile Asp Ser 20 25 30 Asn Asp Ala His Ala Arg Ala Trp Val Glu Ala Leu Arg Glu Ala Gly 35 40 45 Phe Glu Arg Thr Phe Glu Glu Val Arg Pro Leu Ile Gly Met Gly Gly 50 55 60 Asp Gln Leu Ile Pro Arg Leu Thr Gly Leu Asp Ser Gln Ser Glu Glu 65 70 75 80 Gly Gln Arg Leu Thr Gln Gly Trp Leu Arg His Phe Lys Pro Leu Ile 85 90 95 Pro Thr Leu His Ala Thr Arg Gly Ala Arg Ala Leu Val Gln Gly Leu 100 105 110 His Ala Arg Gly Leu Gln Val Leu Leu Ala Thr Ser Gly Glu Ala Glu 115 120 125 Ile Val Asp Glu Leu Leu Lys Gln Ala His Leu Asp Asp Leu Gln Leu 130 135 140 Glu Arg Val Ser Ser Ser Glu Val Glu Ser Ser Lys Pro Ala Pro Asp 145 150 155 160 Leu Val Gln Ala Gly Leu Glu Lys Leu Gly Leu Pro Ala Gly Glu Ala 165 170 175 Leu Leu Val Gly Asp Thr Pro Tyr Asp Ala Gln Ala Ala His Lys Ala 180 185 190 Gly Val Pro Cys Val Leu Leu Arg Cys Gly Gly Asn Thr Gly Leu Glu 195 200 205 Glu His Ala Pro Thr Leu Asp Asp Pro Gln Ala Leu Leu Glu Val Leu 210 215 220 Glu Gly Thr Lys Gly 225 <210> 42 <211> 225 <212> PRT <213> Deinococcus hopiensis <400> 42 Met Ser Leu Lys Asp Leu Leu Ser Pro Ser His Leu Arg Gly Val Leu 1 5 10 15 Leu Asp Val Asp Gly Thr Leu Ile Asp Ser Asn Gly Ala His Ala Arg 20 25 30 Ala Trp Val Gln Ala Leu Arg Asp Glu Gly Phe Ala Arg Thr Phe Glu 35 40 45 Asp Val Arg Pro Leu Ile Gly Met Gly Gly Asp Lys Leu Val Pro Glu 50 55 60 Leu Thr Gly Glu Asp Pro Glu Gly Glu Arg Ala Lys Arg Met Lys Asp 65 70 75 80 Ala Trp Leu Lys His Phe Gln Pro Met Ile Pro Lys Leu Gln Pro Thr 85 90 95 Arg Gly Ala Arg Glu Met Ile Glu Gly Leu Leu Ala Arg Asp Leu Arg 100 105 110 Val Ala Ile Ala Thr Ser Gly Glu Ala Glu Ile Val Glu Gly Leu Leu 115 120 125 Ala Arg Val Gly Val Ala His Leu Lys Leu Asp Arg Val Ser Ser Ser 130 135 140 Glu Val Asp His Ser Lys Pro Asp Pro Asp Leu Ile Gln Val Gly Leu 145 150 155 160 Asn Lys Leu Gly Val Ser Ala Glu Gln Ala Leu Met Val Gly Asp Thr 165 170 175 Pro Phe Asp Ala Glu Ala Ala Arg Lys Ala Gly Val Pro Ser Val Leu 180 185 190 Leu Arg Cys Gly Gly Asp Ala Arg Val Glu Gln His Ala Tyr Val Leu 195 200 205 Asp Asp Pro Arg Ala Leu Leu Glu Ala Leu Pro Asp Gly Ser Gln Thr 210 215 220 Val 225 <210> 43 <211> 214 <212> PRT <213> Deinococcus maricopensis <400> 43 Met Ser Ile Arg Ala Val Ile Met Asp Leu Asp Gly Thr Leu Val Asp 1 5 10 15 Ser Asn Asp Ala His Ala Arg Ala Trp Val Ala Ala Leu Arg Asp Phe 20 25 30 Gly Ile Glu Arg Thr Phe Glu Asp Val Arg Pro Leu Ile Gly Met Gly 35 40 45 Gly Asp Gln Leu Leu Pro Arg Val Ala Asp Val Asp Pro Asn Ser Glu 50 55 60 Arg Gly Glu Gly Leu Thr Gln Ala Trp Ala Glu His Phe Lys Pro Met 65 70 75 80 Ile Pro Glu Leu Arg Ala Thr Arg Gly Ala Arg Glu Leu Leu Glu Ala 85 90 95 Leu His Gly Arg Gly Leu Arg Val Ile Leu Gly Thr Ser Gly Asp Ser 100 105 110 Asp Val Ile Asp Pro Leu Leu Glu His Ile Gly Val Arg Asp Leu Val 115 120 125 Pro Asp Arg Val Thr Ala Asp Asp Val Glu Ala Ser Lys Pro Glu Pro 130 135 140 Asp Ile Leu His Ala Ala Leu Gln Lys Leu Gly Val Gly Ala His Glu 145 150 155 160 Ala Leu Met Val Gly Asp Thr Pro Phe Asp Ala Glu Ala Ala Lys Arg 165 170 175 Ala Asn Val Arg Val Ala Leu Leu Arg Ala Gly Gly Asp Glu Arg Val 180 185 190 Asn Ala Glu Ala Phe Thr Phe Asp Asp Pro Ala Asp Leu Ala Ala His 195 200 205 Leu Asp Asp Val Leu Arg 210 <210> 44 <211> 261 <212> PRT <213> Deinococcus murrayi <400> 44 Met Leu Leu Ala Phe Asp Leu Asp Gly Thr Leu Ile Pro Asp Leu Gly 1 5 10 15 Arg Glu Val Pro Ala Ala Thr Val Arg Ala Leu Ala Arg Leu Arg Gly 20 25 30 Leu Gly Ala Arg Val Ala Val Ile Thr Gly Arg Asp Ala Ala Pro Pro 35 40 45 Gln Val Leu Glu Ala Ala Gln Pro Asp Ala Val Ala Thr Asn Asn Gly 50 55 60 Gly Arg Val Glu Leu Gln Gly Glu Leu His Arg Glu Ala Arg Phe Ser 65 70 75 80 Glu Asp Glu Leu Ala Ala Val Leu Ala His Glu Leu Glu Glu Ala Arg 85 90 95 Val Val Val Phe Arg Pro Ser Gly Leu Tyr Ala Glu Leu Pro Pro Gly 100 105 110 His Ala Pro Glu Ala Trp Met Val Glu Arg Gly Val Arg Pro Leu Ala 115 120 125 Gln Ala Pro Ala Gly Glu Pro Thr Leu Lys Val Gly Phe Tyr His Pro 130 135 140 Gly Val Ala Asp Phe Ala Ala Arg Leu Arg Val Ser His Pro His Leu 145 150 155 160 Val Leu Thr Gly Ala Gln Pro Pro Tyr Ser Glu Phe Leu Thr Val Thr 165 170 175 Pro Ala Gly Ala His Lys Gly Ala Ala Leu Thr Leu Ile Ala Gln Gly 180 185 190 Leu Gly Val Glu Leu Glu Arg Thr Val Val Phe Gly Asp Ser Asp Asn 195 200 205 Asp Val Ala Met Leu Glu Ile Ala Gly Tyr Ala Val Gln Val Gly His 210 215 220 Leu Pro Leu Leu Ala Pro His Ala Asp Asp Arg Val Ser Gly Pro Gln 225 230 235 240 Ala Leu Gly Pro Tyr Leu Glu Glu Leu Ala Asp Arg Leu Glu Ala Ala 245 250 255 Glu Ala Pro Ala Thr 260 <210> 45 <211> 225 <212> PRT <213> Deinococcus murrayi <400> 45 Met Thr His His Asp Leu Leu Ser Pro Glu Arg Leu Arg Gly Val Leu 1 5 10 15 Leu Asp Val Asp Gly Thr Leu Ala Asp Ser Asn His Ala His Ala Arg 20 25 30 Ala Trp Ala Ala Ala Leu Arg Asp Glu Gly Phe Glu Lys Ser Pro Glu 35 40 45 Ala Ile Phe Pro Leu Ile Gly Met Gly Gly Asp Lys Leu Val Pro Glu 50 55 60 Leu Thr Gly Leu Asp Ala Glu Ser Glu Arg Gly Gln Arg Met Thr Glu 65 70 75 80 Gly Trp Val Arg His Phe Gln Glu Leu Ile Pro Asp Leu Arg Pro Thr 85 90 95 Pro Gly Ala Arg Ala Leu Val Glu Gly Leu Arg Ala Arg Gly Leu Arg 100 105 110 Val Val Leu Ala Thr Ser Gly Glu Ala Glu Val Val Asp Ala Leu Leu 115 120 125 Arg Gln Ile Gly Leu Asp Asp Leu Glu Leu Glu Arg Val Ser Ser Thr 130 135 140 Asp Val Glu Asn Ser Lys Pro Asp Pro Asp Leu Val Gln Ala Gly Leu 145 150 155 160 Arg Thr Leu Gly Leu Pro Ala Glu Ala Thr Leu Met Val Gly Asp Thr 165 170 175 Pro Tyr Asp Ala Glu Ala Ala Arg Lys Ala Gly Val Pro Cys Val Leu 180 185 190 Leu Arg Cys Gly Gly His Pro Gly Val Glu Glu His Asp Pro Val Tyr 195 200 205 Asp His Pro Gln Ala Leu Leu Glu Ala Leu Asp Gln Ala Phe Pro Val 210 215 220 Glu 225 <210> 46 <211> 229 <212> PRT <213> Deinococcus reticulitermitis <400> 46 Met Ala Asp Leu Gln Leu Ser Ser Pro Ala Leu Pro Ala Val Ser Arg 1 5 10 15 Leu Lys Gly Leu Leu Leu Asp Leu Asp Gly Thr Leu Val Asp Ser Asn 20 25 30 Gly Ala His Thr Glu Ala Trp Val Gln Ala Leu Ala Asp Gln Gly Phe 35 40 45 Thr Arg Pro Pro Ala Glu Val Arg Pro Leu Ile Gly Met Gly Gly Asp 50 55 60 Gln Leu Val Pro Arg Leu Thr Gly Leu Asp Glu Gly Ser Glu Thr Gly 65 70 75 80 Lys Ala Leu Val Glu Gly Trp Gln Thr His Phe Lys Ser Arg Met Pro 85 90 95 Ser Val Arg Thr Phe Pro Gly Ala Arg Glu Leu Leu Glu Trp Ala Arg 100 105 110 Glu Val Gly Leu Pro Val Val Leu Ala Ser Ser Gly Glu Asp Glu Ile 115 120 125 Val Glu Gly Leu Leu Ala Gln Ala Gly Leu Leu Glu Leu Val Thr Asp 130 135 140 Arg Val Arg Ser Asp Glu Val Asn Gln Thr Lys Pro Arg Pro Asp Val 145 150 155 160 Val Gln Ala Ala Leu Lys Lys Ala Gly Val Ala Pro Glu Glu Ala Leu 165 170 175 Phe Val Gly Asp Thr Val His Asp Ala Arg Ala Ala Arg Ala Ala Gly 180 185 190 Val Pro Cys Val Leu Val Arg Ala Gly Gly Ser Pro Gly Leu Asp Ala 195 200 205 Glu Pro His Val Leu Gly Asp Leu Met Glu Leu Leu Ala Ala Leu Lys 210 215 220 Ala Gly Ser Thr Thr 225 <210> 47 <211> 227 <212> PRT <213> Deinococcus wulumuqiensis <400> 47 Met Arg Leu Ser Pro Gln Thr Pro Ala Leu Pro Ala Val Pro Asp Leu 1 5 10 15 Arg Ala Leu Leu Leu Asp Leu Asp Gly Thr Leu Ala Asp Ser Asn Asp 20 25 30 Ala His Ala Arg Ala Trp Val Gln Ala Leu Ala Asp Gln Asp Ile Thr 35 40 45 Arg Glu Glu Ser Glu Val Arg Pro Leu Ile Gly Met Gly Gly Asp Gln 50 55 60 Leu Val Pro Ala Leu Thr Gly Glu Ser Asp Arg Ser Glu Leu Gly Lys 65 70 75 80 Ala Leu Val Gln Gly Trp Gln Asp His Phe Gly Pro Met Ile Pro Glu 85 90 95 Ile Ala Pro Leu Gly Gly Ala Arg Glu Leu Leu Glu Trp Ala Arg Thr 100 105 110 Gln Gly Leu Lys Val Val Leu Ala Ser Ser Gly Glu Asp Asp Ile Val 115 120 125 Asp Ala Leu Leu Glu Gln Ile Gly Val Gly Asp Leu Val Pro Leu Arg 130 135 140 Val Arg Ser Asp Glu Val Gln Arg Thr Lys Pro Ala Pro Asp Val Leu 145 150 155 160 Gln Ala Ala Leu Ala Lys Ala Gly Val Ser Ala Ala Gln Ala Leu Phe 165 170 175 Val Gly Asp Thr Thr Tyr Asp Ala Arg Ala Ala Arg Ala Ala Gly Val 180 185 190 Ala Cys Val Val Leu Arg Ala Gly Gly Ser Pro Gly Leu Asn Thr Glu 195 200 205 Pro His Val Leu Ser Asp Pro Ala Glu Leu Leu Ala Glu Leu Lys Lys 210 215 220 Ala Thr Ser 225 <210> 48 <211> 224 <212> PRT <213> Deinococcus sp. Leaf326 <400> 48 Met Ser Ser Tyr Leu Gly Val Ile Phe Asp Leu Asp Gly Thr Leu Val 1 5 10 15 Asp Ser Asn Asp Gly His Ala Arg Ala Trp Val Arg Ala Phe Ala Asp 20 25 30 Gln Gly Ile Glu Val Thr Phe Gly Gln Val Arg Pro Leu Met Gly Met 35 40 45 Gly Gly Asp Gln Leu Val Pro His Leu Thr Gly Ile Gly Lys Asp Asp 50 55 60 Pro Arg Tyr Glu Ala Leu Ser Asp Gly Trp Lys Arg His Phe Gln Ala 65 70 75 80 Glu Glu Leu Pro Arg Leu Arg Ala Gln Pro Gly Val Arg Pro Leu Leu 85 90 95 Val Ala Leu Gln Ala Gln Gly Leu Arg Leu Ile Val Gly Thr Ser Ala 100 105 110 Asp Glu Ala Leu Val Arg Asp Leu Leu Gly Val Ala Gly Ala Gln Asp 115 120 125 Leu Phe Thr Glu Tyr Thr Thr Ala Ser Glu Val Glu Glu Ser Lys Pro 130 135 140 Gln Pro Asp Ile Val Gln Ala Ala Val Arg Lys Leu Gly Leu Asp Pro 145 150 155 160 Ala Gln Val Leu Met Val Gly Asp Thr Ser Phe Asp Ile Glu Ser Ala 165 170 175 Arg Gly Ala Gly Val Ala Thr Val Ala Leu Arg Ser Gly Gly Thr Ala 180 185 190 Gln Phe Thr Gly Ala Ala Ala Val Tyr Asp Ser Pro Gln Asp Trp Leu 195 200 205 Asp His Leu Ala Ala Ser Pro Leu Gly Pro Asp Gln Pro Ser Ala Ser 210 215 220 <210> 49 <211> 225 <212> PRT <213> Deinococcus phoenicis <400> 49 Met Thr Pro Ser Leu Glu Ala Ala Leu Asn Pro Asp Arg Leu Arg Gly 1 5 10 15 Val Leu Leu Asp Val Asp Gly Thr Leu Ile Asp Ser Asn Asp Ala His 20 25 30 Ala Arg Ala Trp Val Glu Ala Leu Arg Asp Glu Gly Phe Glu Lys Thr 35 40 45 Val Gly Glu Val Arg Pro Leu Ile Gly Met Gly Gly Asp Gln Met Ile 50 55 60 Pro Arg Leu Thr Gly Lys Gly Ser Glu Ser Glu Val Gly Gln Arg Leu 65 70 75 80 Thr Asp Gly Trp Leu Lys His Phe Lys Pro Leu Ile Pro Glu Leu His 85 90 95 Ala Thr Arg Gly Ala Arg Ala Leu Val Glu Gly Leu Arg Ala Arg Gly 100 105 110 Leu Arg Val Val Leu Ala Thr Ser Gly Glu Ala Glu Val Val Asp Ala 115 120 125 Leu Leu Lys Gln Ala Gly Leu Ala Asp Leu Asn Leu Asp Arg Val Ser 130 135 140 Ser Ser Glu Val Glu Ser Ser Lys Pro Ala Pro Asp Leu Ile Gln Val 145 150 155 160 Gly Leu Lys Lys Leu Gly Val Pro Ala Glu Ala Ala Leu Met Val Gly 165 170 175 Asp Thr Pro Tyr Asp Ala Glu Ala Ala Ala Lys Ala Asp Val Pro Cys 180 185 190 Ile Leu Leu Arg Cys Gly Gly Asp Thr Asp Leu Glu Lys His Ala Pro 195 200 205 Val Leu Asp Asp Pro Gln Ala Leu Leu Glu Ala Leu Glu Lys Ala Thr 210 215 220 Val 225 <210> 50 <211> 228 <212> PRT <213> Deinococcus proteolyticus <400> 50 Met Gln Leu Asn Pro Gln Gly Pro Ser Leu Pro Thr Ile Ala Asp Leu 1 5 10 15 Arg Ala Leu Phe Val Asp Leu Asp Gly Thr Leu Val Asp Ser Asn Asp 20 25 30 Ala His Ala Arg Ala Trp Val Gln Ala Leu Ala Asp Gln Asp Ile Thr 35 40 45 Arg Asp Gln Gly Glu Val Arg Pro Leu Ile Gly Met Gly Gly Asp Gln 50 55 60 Leu Val Pro Glu Leu Thr Gly Lys Ser Asp Ser Ser Glu Leu Gly Glu 65 70 75 80 Ala Leu Val Gln Gly Trp Gln Asp His Phe Lys Pro Leu Ile Ala Gly 85 90 95 Leu Glu Val Leu Pro Gly Ala Arg Glu Leu Leu Glu Trp Ala Arg Gly 100 105 110 Gln Gly Leu Thr Val Val Leu Ala Ser Ser Gly Glu Asp Asp Ile Val 115 120 125 Glu Ala Leu Leu Glu His Ala Gly Leu Thr Asp Leu Ile Asp Leu Arg 130 135 140 Val Arg Ser Asp Glu Val Gln Gln Thr Lys Pro Glu Pro Asp Ile Leu 145 150 155 160 Gln Ala Ala Leu Asn Lys Ala Gly Val Gln Pro Gln Gln Ala Leu Phe 165 170 175 Val Gly Asp Thr Arg Phe Asp Ala Glu Ala Ala Gln Lys Ala Gly Val 180 185 190 Pro Cys Val Leu Leu Arg Ala Gly Gly Ser Pro Asp Leu Glu Glu Ala 195 200 205 Gln Tyr Val Leu Ala Asn Ala His Glu Leu Leu Ser Ala Leu Gln Ala 210 215 220 Ala Thr Gln Asp 225 <210> 51 <211> 216 <212> PRT <213> Deinococcus sp. 17bor-2 <400> 51 Met Thr Thr Ser Leu Gln Ala Ala Ile Leu Asp Leu Asp Gly Thr Leu 1 5 10 15 Ile Asp Ser Asn Asp Ala His Ala Arg Ala Trp Val Thr Ser Leu Lys 20 25 30 Arg His Gly Phe Asp Ile Pro Phe Glu Arg Val Arg Pro Leu Ile Gly 35 40 45 Met Gly Gly Asp Lys Leu Ile Pro Glu Leu Thr Gly Leu Asp Pro Glu 50 55 60 Ser Glu Thr Gly Lys Ala Leu Thr Gln Gly Trp Gly Asp Ala Phe Lys 65 70 75 80 Pro Met Ile Pro Ser Leu Gln Ala Thr Pro Gly Ala Arg Glu Leu Val 85 90 95 Ala Gly Leu Arg Glu Leu Gly Trp Lys Leu Met Leu Gly Ser Ser Gly 100 105 110 Glu Asp Glu Val Val Glu Gln Glu Leu Glu His Leu Gly Leu Glu Asp 115 120 125 Leu Lys Asn Ser Arg Val Thr Ser Ser Glu Val Glu Glu Ser Lys Pro 130 135 140 Asp Ala Asp Ile Leu Ala Val Ala Leu Arg Lys Leu Gly Val Pro Ala 145 150 155 160 Gly Ala Ala Leu Met Val Gly Asp Thr Lys Tyr Asp Ala Glu Ala Ala 165 170 175 Arg Arg Ala Gly Val Arg Cys Val Leu Leu Arg Cys Gly Gly Asn Pro 180 185 190 Asp Leu Pro Gly Glu Val Tyr Ala Ser Pro Ala Asp Leu Leu Gly Ala 195 200 205 Leu Arg Ser Gly Asp Trp Gly Arg 210 215 <210> 52 <211> 225 <212> PRT <213> Deinococcus sp. NW-56 <400> 52 Met Thr Gln His Asp Leu Leu Ala Pro Asp Arg Leu Arg Gly Val Leu 1 5 10 15 Leu Asp Val Asp Gly Thr Leu Ala Asp Ser Asn Leu Ala His Ala Arg 20 25 30 Ala Trp Ala Ala Ala Leu Ala Asp Glu Gly Phe Glu Lys Thr Pro Glu 35 40 45 Asp Ile Phe Pro Leu Ile Gly Met Gly Gly Asp Lys Leu Val Pro Glu 50 55 60 Leu Thr Gly Leu Asp Ala Glu Ser Glu Arg Gly Gln Arg Leu Thr Asp 65 70 75 80 Gly Trp Val Arg His Phe Arg Glu Leu Ile Pro Asp Leu Arg Pro Thr 85 90 95 Pro Gly Ala Arg Ala Leu Ile Glu Gly Leu Arg Ala Arg Gly Leu Arg 100 105 110 Val Ala Leu Ala Thr Ser Gly Glu Ala Glu Ile Val Asp Ala Leu Leu 115 120 125 Glu Gln Ile Gly Leu Asp Asp Leu Lys Leu Glu Arg Val Ser Ser Ser 130 135 140 Asp Val Glu Asn Ser Lys Pro Asp Pro Asp Leu Val Lys Ala Gly Leu 145 150 155 160 His Thr Leu Gly Leu Pro Ala Gly Ala Ala Leu Met Val Gly Asp Thr 165 170 175 Pro Tyr Asp Ala Glu Ala Ala Arg Gly Ala Gly Val Pro Cys Val Leu 180 185 190 Leu Arg Cys Gly Gly His Pro Gly Ala Glu Gly His Asp Pro Leu Tyr 195 200 205 Asp Asp Pro Gln Ala Leu Leu Ala Ala Leu Asn Glu Ala Phe Pro Val 210 215 220 Glu 225 <210> 53 <211> 225 <212> PRT <213> Deinococcus sp. RL <400> 53 Met Thr Gln Gly Asp Leu Leu Ser Pro Lys Arg Leu Arg Gly Val Leu 1 5 10 15 Leu Asp Val Asp Gly Thr Leu Ala Asp Ser Asn Met Ala His Ala Arg 20 25 30 Ala Trp Ala Ala Ala Leu Arg Asp Glu Gly Phe Glu Gln Ser Pro Glu 35 40 45 Ala Ile Leu Pro Leu Ile Gly Met Gly Gly Asp Lys Leu Val Pro Glu 50 55 60 Leu Thr Gly Leu Asp Ala Glu Ser Glu Arg Gly Gln Arg Leu Thr Asp 65 70 75 80 Gly Trp Ala Arg His Phe Arg Ala Leu Ile Pro Asp Leu Arg Pro Thr 85 90 95 Pro Gly Ala Arg Ala Leu Leu Glu Gly Leu Arg Ala Arg Gly Leu Arg 100 105 110 Val Ala Leu Ala Thr Ser Gly Glu Ala Glu Val Val Asp Ala Leu Leu 115 120 125 Gln Gln Ile Gly Leu Asp Asp Leu Glu Leu Glu Arg Val Ser Ser Ser 130 135 140 Asp Val Glu Asn Ser Lys Pro Asp Pro Asp Leu Val Gln Ala Gly Leu 145 150 155 160 Arg Thr Leu Gly Leu Pro Ala Glu Ala Thr Leu Met Val Gly Asp Thr 165 170 175 Pro Tyr Asp Ala Glu Ala Ala Arg Lys Ala Gly Val Pro Cys Val Leu 180 185 190 Leu Arg Cys Gly Gly His Pro Gly Val Glu Glu His Asp Pro Val Tyr 195 200 205 Asp His Pro Gln Ala Leu Leu Glu Ala Leu Asp Gln Ala Phe Pro Val 210 215 220 Glu 225 <210> 54 <211> 198 <212> PRT <213> Deinococcus sp. RL <400> 54 Met Ala His Ala Arg Ala Trp Ala Ala Ala Leu Arg Asp Glu Gly Phe 1 5 10 15 Glu Gln Ser Pro Glu Ala Ile Leu Pro Leu Ile Gly Met Gly Gly Asp 20 25 30 Lys Leu Val Pro Glu Leu Thr Gly Leu Asp Ala Glu Ser Glu Arg Gly 35 40 45 Gln Arg Leu Thr Asp Gly Trp Ala Arg His Phe Arg Ala Leu Ile Pro 50 55 60 Asp Leu Arg Pro Thr Pro Gly Ala Arg Ala Leu Leu Glu Gly Leu Arg 65 70 75 80 Ala Arg Gly Leu Arg Val Ala Leu Ala Thr Ser Gly Glu Ala Glu Val 85 90 95 Val Asp Ala Leu Leu Gln Gln Ile Gly Leu Asp Asp Leu Glu Leu Glu 100 105 110 Arg Val Ser Ser Ser Asp Val Glu Asn Ser Lys Pro Asp Pro Asp Leu 115 120 125 Val Gln Ala Gly Leu Arg Thr Leu Gly Leu Pro Ala Glu Ala Thr Leu 130 135 140 Met Val Gly Asp Thr Pro Tyr Asp Ala Glu Ala Ala Arg Lys Ala Gly 145 150 155 160 Val Pro Cys Val Leu Leu Arg Cys Gly Gly His Pro Gly Val Glu Glu 165 170 175 His Asp Pro Val Tyr Asp His Pro Gln Ala Leu Leu Glu Ala Leu Asp 180 185 190 Gln Ala Phe Pro Val Glu 195 <210> 55 <211> 223 <212> PRT <213> Deinococcus sp. YIM 77859 <400> 55 Met Ile Ala Glu Glu Leu Leu Ser Pro Asp Arg Leu Arg Gly Val Leu 1 5 10 15 Leu Asp Val Asp Gly Thr Leu Val Asp Ser Asn Asp Ala His Ala Tyr 20 25 30 Ala Trp Leu Glu Ala Leu Arg Glu Glu Gly Phe Thr Arg Thr Phe Glu 35 40 45 Glu Val Arg Pro Leu Ile Gly Met Gly Gly Asp Gln Leu Val Pro Arg 50 55 60 Leu Thr Gly Glu Asp Gly Glu Ser Glu Arg Gly Lys Arg Leu Thr Arg 65 70 75 80 Gly Trp Leu Asn His Phe Lys Pro Arg Ile Pro Gln Leu Gln Ala Thr 85 90 95 Arg Gly Ala Arg Ala Leu Val Glu Gly Leu Gln Ala Arg Gly Leu Arg 100 105 110 Val Val Leu Ala Thr Ser Gly Glu Ala Asp Ile Val Asp Gly Leu Leu 115 120 125 Lys Arg Ala His Leu Glu Asp Leu Asn Leu Asp Arg Val Ser Ser Ser 130 135 140 Glu Val Glu Ser Ser Lys Pro Ala Pro Asp Leu Ile Gln Ala Gly Leu 145 150 155 160 Lys Lys Leu Gly Leu Pro Ala Glu Ala Ala Leu Met Val Gly Asp Thr 165 170 175 Pro Tyr Asp Ala Glu Ala Ala Arg Lys Ala Gly Val Pro Tyr Val Leu 180 185 190 Leu Arg Cys Gly Gly Asn Thr Gly Leu Glu Glu His Gly Pro Thr Leu 195 200 205 Asp Asp Pro Gln Ala Leu Leu Glu Ala Leu Glu Lys Ala Ser Ala 210 215 220 <210> 56 <211> 240 <212> PRT <213> Desulfurococcus mucosus <400> 56 Met Ala Val Lys Ile Val Phe Thr Asp Ile Asp Gly Thr Leu Thr Val 1 5 10 15 Asn Arg Glu Ser Tyr Arg Leu Ala Val Glu Ala Val Glu Ala Leu Arg 20 25 30 Met Leu Val Asp Lys Gly Val Ile Val Ser Leu Val Ser Ser Asn Ala 35 40 45 Leu Pro Val Val Val Ala Leu Ser Arg Tyr Ile Gly Leu Asn Gly Pro 50 55 60 Val Val Gly Glu Ser Gly Ala Leu Val Tyr His Asp Glu Trp Gly Leu 65 70 75 80 Val Glu Leu Ala Gly Glu Ser Ala Arg Gln Val Tyr Met Asp Leu Leu 85 90 95 Gly Asn Tyr Arg Glu Tyr Val Glu Asp Ser Trp Gln Asn Lys Phe Arg 100 105 110 Leu Tyr Glu Tyr Ala Leu Lys Leu Arg Arg Glu His Arg Gly Arg Ala 115 120 125 Arg Glu Val Val Asp Glu Leu Arg Arg Tyr Val Glu Ser Lys Tyr Lys 130 135 140 Gly Phe Thr Val Asp Tyr Ser Gly Tyr Ala Ile His Val His Ala Lys 145 150 155 160 Gly Val Gly Lys Gly Val Ala Val Asp Tyr Ile Leu Arg Arg Leu Gly 165 170 175 Leu Ser Gly Gly Glu Ala Leu Gly Ile Gly Asp Ser Tyr Met Asp Val 180 185 190 Asp Phe Ile Ala Arg Leu Gly Tyr Arg Ala Ala Val Gly Gly Ala Asp 195 200 205 Glu Glu Leu Val Arg Val Cys Asn Ile Val Ala Glu Ala Pro Ser Gly 210 215 220 Leu Gly Val Ala Glu Val Val His Arg Ile Leu Gly Glu Arg His Glu 225 230 235 240 <210> 57 <211> 230 <212> PRT <213> Desulfurococcus mucosus <400> 57 Met Asn Ser Leu Leu Arg Gly Ile Arg Leu Ile Leu Phe Asp Leu Asp 1 5 10 15 Gly Thr Ile Val Asp Ser Glu Asp Phe Ile Val Trp Ser Phe Val Glu 20 25 30 Ala Gly Arg Leu Thr Gly Ile Arg Val Asp Pro Leu Lys Val Arg Glu 35 40 45 Leu Ile Gly Phe Pro Leu Glu Ser Ile Leu Glu Ala Val Val Gly Thr 50 55 60 Gly Leu Ser Arg Glu Asp Ala Ala Arg Phe Ile Glu Val Arg Arg Lys 65 70 75 80 Leu Val Gln Glu Asn Trp Phe Lys His Val Arg Leu Phe Pro Asp Val 85 90 95 Ile Pro Val Leu Arg Gln Leu Ser Ser Thr Gly Tyr Met Leu Gly Val 100 105 110 Ala Ser Ser Ser Ile Arg Glu Arg Val Glu Leu Phe Leu Ser His Leu 115 120 125 Gly Val Ser Gly Tyr Phe Lys Val Val Ser Gly Leu Glu Pro Gly Val 130 135 140 Lys Gly Lys Pro Glu Pro Asp Val Ile Val Asn Ala Leu Lys Ala Ala 145 150 155 160 Gly Val Pro Arg Ser Glu Ala Leu Tyr Val Gly Asp Arg Met Val Asp 165 170 175 Cys Ile Ala Ala Arg Arg Ala Gly Val Lys Val Val Ile Val Asp Arg 180 185 190 Gly Ser Val Asn Arg Trp Lys Gln Glu Glu Cys Val Pro Asp Ala Trp 195 200 205 Ile Ser Ser Leu Leu Glu Leu Val Asp Asp Ala Ser Thr Pro Thr Arg 210 215 220 Glu Ala Thr Arg His Asp 225 230 <210> 58 <211> 224 <212> PRT <213> Desulfurococcus mucosus <400> 58 Met Arg Ile Arg Leu Val Val Met Asp Tyr Asp Leu Ser Met Val Tyr 1 5 10 15 Asn Leu Ser Asp Phe Tyr Glu Ala Tyr Ser Gly Ala Leu Lys Ser Tyr 20 25 30 Gly Gly Gly Phe Ile Ser Phe Asp Glu Phe Leu Asn Leu Leu Asn Glu 35 40 45 Asp Arg Leu Ser Glu Arg Ile Pro Gly Gly Val Arg Glu Glu Glu Phe 50 55 60 Trp Leu Leu Phe Arg Arg Ile Tyr Val Ser Arg His Pro Ile Pro Met 65 70 75 80 Asp Gly Val Val Asp Leu Leu Arg Leu Leu Lys Ser Leu Pro Val Lys 85 90 95 Ile Ala Val Val Ser Gly Arg Glu Thr Ser Gly Glu Tyr Ile Trp Arg 100 105 110 Asp Leu Arg Met Leu Gly Leu Asp Glu Tyr Val Asp Glu Val Tyr Thr 115 120 125 Val Ser Asp Leu Gln Arg Leu Asn Gly Leu Glu Glu Tyr Leu Phe Asp 130 135 140 Lys Ser Trp Ile Ile Ser Tyr Ile Leu Arg Lys His Gly Val Ala Pro 145 150 155 160 Cys Asn Ala Leu Cys Ile Gly Asp Phe Thr Thr Asp Leu Leu Ser Cys 165 170 175 Arg Lys Leu Gly Ile Pro Phe Ile Gly Val Asn Lys Asp Glu Tyr Arg 180 185 190 Gly Met Leu Leu Lys Lys Arg Gly Ala Ala Ile Val Val Lys Asn Leu 195 200 205 Phe Glu Val Val Gln Trp Ile Pro Glu Leu Glu Lys Asp Ser Thr Cys 210 215 220 <210> 59 <211> 265 <212> PRT <213> Dictyoglomus turgidum <400> 59 Met Lys Arg Ile Leu Glu Val Ala Ile Lys Thr Ile Lys Glu Ser Gly 1 5 10 15 Asn Ile Leu Leu Asn Tyr Ile Gly Glu Glu Lys Glu Ile Glu Leu Lys 20 25 30 Gly Ile Ser Asn Leu Val Thr Gln Val Asp Lys Leu Ser Glu Arg His 35 40 45 Ile Leu Lys Ser Ile Glu Glu Asn Phe Pro Asp His Ser Ile Leu Thr 50 55 60 Glu Glu Thr Gly Phe Ile Asn Lys Asn Ser Glu Tyr Thr Trp Ile Val 65 70 75 80 Asp Pro Leu Asp Gly Thr Thr Asn Tyr Ala His Asn Phe Pro Phe Phe 85 90 95 Gly Ile Ser Ile Ala Leu Ile Lys Asn Lys Glu Ile Ile Leu Gly Leu 100 105 110 Ile Tyr Asp Pro Ile Arg Asp Glu Leu Phe Tyr Ala Ile Lys Asn Glu 115 120 125 Gly Ala Tyr Leu Asn Asp Arg Arg Ile Glu Val Ser Lys Thr Glu Ser 130 135 140 Leu Glu Asn Ser Leu Ile Ser Phe Ala Phe Pro Tyr Glu Leu Ser Leu 145 150 155 160 Glu Glu Lys Asn Phe Ile Pro Phe Ile Asn Phe Ser Ser Arg Thr His 165 170 175 Gly Ile Arg Arg Thr Gly Ser Ala Ala Ile Glu Ile Ala Tyr Val Gly 180 185 190 Cys Gly Arg Leu Asp Gly Phe Trp Ala Lys Lys Leu Lys Pro Trp Asp 195 200 205 Ile Ser Ala Gly Ile Leu Ile Val Glu Glu Ala Lys Gly Lys Val Thr 210 215 220 Asp Phe Ser Gly Asn Asn Ile Asp Ile His Thr Asp Asn Ile Leu Phe 225 230 235 240 Ser Asn Gly Lys Ile His Gln Glu Met Ile Lys Ile Leu Asn Leu Gly 245 250 255 Lys Ile Phe Ile Arg Asn Glu Lys Phe 260 265 <210> 60 <211> 249 <212> PRT <213> Dictyoglomus turgidum <400> 60 Met Tyr Lys Leu Phe Ile Thr Asp Leu Asp Gly Ser Ile Met Asp Gly 1 5 10 15 Ser Glu Val Ile Ser Thr Lys Asn Leu Lys Ala Ile Lys Ile Leu Arg 20 25 30 Glu Asn Asn Ile Glu Ile Thr Ile Ala Thr Gly Arg Arg Trp Ser Ser 35 40 45 Ile Ser Lys Ile Val Glu Pro Leu Ser Leu Thr Leu Pro Val Ile Leu 50 55 60 Tyr Asn Gly Ala Gly Ile Tyr Asp Pro Leu Arg Lys Ser Phe Leu Tyr 65 70 75 80 Leu Gln His Leu Ser Lys Glu Glu Val Ile Gly Ala Leu Lys Val Ile 85 90 95 His Tyr Tyr Leu Ser Phe Val Lys Leu Gly Ile Tyr His Asp Asp Lys 100 105 110 Leu Tyr Glu Asp Ser Glu Ala Leu Glu Phe Leu Lys Asp Asn Lys Asn 115 120 125 Gly Ile Ile Lys Ile Phe Ile Glu Gly Glu Lys Glu Leu Leu Lys Glu 130 135 140 Leu Lys Asn Arg Leu Glu Lys Ile Tyr Leu Asn Val Val Phe Ser Ser 145 150 155 160 Tyr Lys Tyr Leu Glu Ile Leu Pro Lys Gly Ala Ser Lys Gly Lys Ala 165 170 175 Leu Lys Lys Leu Leu Lys Asn Leu Asp Ile Lys Leu Glu Glu Val Ile 180 185 190 Ala Leu Gly Asp Tyr Asp Asn Asp Glu Glu Met Leu Lys Leu Ser Gly 195 200 205 Leu Gly Ile Thr Leu Lys Asn Ala Ser Glu Arg Leu Lys Lys Ile Ala 210 215 220 Asp Tyr Val Ile Asp Ala Ser Pro Ser Glu Ser Val His Tyr Ile Ile 225 230 235 240 Gln Gln Val Leu Asn Phe Glu Arg Arg 245 <210> 61 <211> 213 <212> PRT <213> Dictyoglomus turgidum <400> 61 Met Glu Leu Lys Gly Ile Ile Phe Asp Trp Asp Gly Thr Leu Val Asp 1 5 10 15 Ser Phe Pro Ala Cys Ile Lys Ala Thr Lys Glu Ile Phe Lys Lys Phe 20 25 30 Gly Phe Ile Ile Ser Glu Glu Glu Tyr Arg Glu Lys Phe Ser Pro Asn 35 40 45 Trp Tyr Asp Ile Tyr Arg Lys His Asn Ile Pro Glu Lys Tyr Trp Lys 50 55 60 Glu Ile Asp Leu Leu Trp Lys Asp Tyr Phe Asp Tyr Ser Leu Val Lys 65 70 75 80 Trp Arg Glu Gly Ala Val Glu Asn Leu Thr Phe Leu Lys Lys Leu Asn 85 90 95 Leu Lys Ile Gly Val Val Thr Ala Ser Thr Lys Glu Asp Ile Glu Arg 100 105 110 Glu Lys Pro Tyr Leu Asn Pro Glu Glu Tyr Ile Asp Gly Trp Ile Thr 115 120 125 Trp Glu Asp Ser Glu Lys Pro Lys Pro Asp Pro Leu Pro Leu Leu Lys 130 135 140 Ile Leu Lys Lys Leu Ser Leu Ser Pro Leu Glu Val Ile Tyr Val Gly 145 150 155 160 Asp Thr Lys Glu Asp Ile Glu Met Ser Lys Lys Leu Asn Ile Leu Thr 165 170 175 Ile Gly Val Leu Ser Pro Phe Thr Gln Lys Glu Lys Leu Val Ser Ser 180 185 190 Asn Pro Asn Phe Leu Phe Asn Asn Leu Phe Glu Leu Leu Lys Phe Trp 195 200 205 Lys Asp Leu Phe Leu 210 <210> 62 <211> 213 <212> PRT <213> Effusibacillus pohliae <400> 62 Met Lys Tyr Pro Tyr Ile Leu Phe Asp Leu Asp Gly Thr Leu Val Asp 1 5 10 15 Thr Asn Glu Leu Ile Leu Gln Ser Phe Glu His Thr Leu Glu His Tyr 20 25 30 Phe Pro Gly Arg Tyr Ser Arg Gln Asp Val Leu Pro Tyr Met Gly Glu 35 40 45 Pro Leu Val Lys Gln Phe Ala Arg Trp Ala Pro Pro Glu Gln Val Ser 50 55 60 Glu Leu Val Gln Ala Tyr Arg Lys Tyr Asn Val Glu Asn His Asp Arg 65 70 75 80 Leu Ala Thr Ile Phe Pro Asn Val Pro Glu Val Leu Ala Glu Leu Lys 85 90 95 Ala Gln Gly Cys Arg Met Ala Val Val Thr Asn Lys Met Arg Leu Thr 100 105 110 Ala Glu Arg Gly Leu Arg Leu Phe Gly Leu Asp Arg Tyr Leu Glu Thr 115 120 125 Val Val Thr Val Glu Asp Thr Asp Gln His Lys Pro His Pro Ala Pro 130 135 140 Leu Gln Ile Ala Ile Glu Arg Leu Gly Gly Glu Pro Ala Gly Thr Leu 145 150 155 160 Met Val Gly Asp Ser Pro Tyr Asp Ile Leu Ala Gly Gln Ala Ala Gly 165 170 175 Thr Ala Thr Cys Ala Val Ala Trp Ser Leu Arg Gly Cys Glu Gly Leu 180 185 190 Ala Ser Tyr Lys Pro Asp Tyr Leu Ile Asp Glu Met Arg Glu Leu Leu 195 200 205 Glu Ile Val Arg Gly 210 <210> 63 <211> 215 <212> PRT <213> Fervidobacterium gondwanense <400> 63 Met Arg Pro Lys Ala Cys Ile Phe Asp Leu Asp Gly Val Ile Val Asp 1 5 10 15 Thr Ala Lys Tyr His Tyr Leu Ala Trp Lys Arg Leu Ala Lys Glu Leu 20 25 30 Gly Phe Glu Phe Thr Glu Lys Asp Asn Glu Arg Leu Lys Gly Val Ser 35 40 45 Arg Met Glu Ser Leu Glu Ile Leu Leu Ser Val Gly Gly Ile Lys Ile 50 55 60 Glu Asp Gln Lys Leu Lys Glu Gln Leu Ser Asp Lys Lys Asn Asn Trp 65 70 75 80 Tyr Val Glu Tyr Ile Ser Gln Met Thr Lys Asp Glu Ile Leu Pro Gly 85 90 95 Val Glu Glu Phe Leu Arg Lys Leu Lys Asn Ala Gly Ile Lys Ile Ala 100 105 110 Ile Gly Ser Ala Ser Lys Asn Thr Met Thr Ile Leu Arg Arg Ile Glu 115 120 125 Leu Val Asp Leu Phe Asp Ser Ile Ile Asp Gly Thr Lys Ile Thr Lys 130 135 140 Ala Lys Pro Asp Pro Glu Val Phe Leu Lys Ala Ala Glu Glu Leu Asn 145 150 155 160 Val Asp Pro Lys Asp Cys Cys Val Phe Glu Asp Ala Val Ala Gly Ile 165 170 175 Glu Ala Ala Lys Arg Ala Gly Met Lys Val Ile Gly Val Gly Asp His 180 185 190 Glu Ile Leu Lys Asp Ala Asp Arg Val Ile Thr Ser Phe Val Gly His 195 200 205 Gly Ile Glu Leu Val Glu Phe 210 215 <210> 64 <211> 305 <212> PRT <213> Fervidobacterium islandicum <400> 64 Met Lys Glu Trp Arg Glu Glu Glu Leu Ser Asn Lys Ser Lys Cys Lys 1 5 10 15 Arg Thr Phe Val Phe Asp Leu Asp Gly Thr Val Leu Asn Ser Lys Asn 20 25 30 Glu Phe Pro Lys Glu Thr Lys Thr Leu Val Gln Asn Ile Val Gly Asn 35 40 45 Gly Asp Asn Val Val Phe Ala Thr Gly Arg Met His Ile Ser Ala Lys 50 55 60 Lys Leu Leu Asp Asn Val Phe Gly Glu Asp Ile Phe Pro Ile Ile Ser 65 70 75 80 Tyr Asn Gly Ala Val Ile Tyr Val Pro Gly Glu Gly Phe Ile Tyr Glu 85 90 95 Lys Thr Leu Asp Ile Glu Thr Ala Tyr Lys Val Ile Asp Phe Leu Arg 100 105 110 Glu Lys Asn Ile His Ile Gln Thr Tyr Val Asp Asp Asn Leu Tyr Ser 115 120 125 Glu Lys Asp Asp Asp Glu Ile Arg Leu Tyr Ala Lys His Ala Asp Val 130 135 140 Pro Tyr Tyr Val Val Glu Asp Leu Lys Ala Leu Glu His Pro Pro Ile 145 150 155 160 Lys Met Leu Ala Ile Ala Glu Glu Ala Ile Leu Asp Ser Leu Ile Glu 165 170 175 Pro Leu Lys Glu Ile Val Asp Gly Lys Ala Asn Val Phe Lys Ser Phe 180 185 190 Pro Ile Phe Leu Asp Ile Val Pro Ala Asp Ala Asn Lys Gly Ile Ala 195 200 205 Leu Lys Phe Leu Ala Asp Tyr Leu Asn Phe Asp Leu Gly Ser Thr Ile 210 215 220 Val Phe Gly Asp Asn Glu Asn Asp Ile Tyr Met Phe Lys Val Ala Ser 225 230 235 240 Lys Arg Ile Ala Val Ser Asn Ala Val Trp Arg Leu Lys Glu Val Ala 245 250 255 Asp Phe Val Ser Lys Ser Asn Glu Glu Asn Gly Val Leu His Ala Phe 260 265 270 Val His Leu Phe Pro Glu Tyr Val Arg Gly Ile Asp Phe Ser Ser Gly 275 280 285 His Thr Asn Ser Asn Ile Val Asn Thr Thr Ser Asp Asn Lys Ala Ala 290 295 300 Glu 305 <210> 65 <211> 278 <212> PRT <213> Fervidobacterium islandicum <400> 65 Met Val Lys Leu Ile Val Thr Asp Leu Asp Gly Thr Leu Leu Asn Asp 1 5 10 15 Glu Lys His Ile Pro Lys Glu Asn Ile Glu Val Leu Arg Ala Ala Met 20 25 30 Glu Lys Gly Ile His Val Ser Val Ala Thr Gly Arg Asn Phe Tyr Ser 35 40 45 Ala Lys Pro Tyr Val Glu Glu Leu Gly Leu Asp Val Pro Val Ile Phe 50 55 60 Gln Asn Gly Ala Phe Ile Tyr Gln Trp Met Glu Asn Lys Ile Ile Tyr 65 70 75 80 Lys Ser Ala Leu Gly Ser Glu Ile Ala Thr Lys Val Ile Glu Thr Ala 85 90 95 Arg Arg His Lys Leu Phe Tyr Ile Leu Tyr Arg Asp Phe Leu Glu Glu 100 105 110 Lys Asp Met Tyr Ile Asp Met Pro Tyr Thr Gly Ser Phe Ser Leu Tyr 115 120 125 Leu Gly Gln Asn Gln Trp Arg Leu Asn Ile Val Asp Asp Val Leu Lys 130 135 140 Tyr Ile Lys Thr Lys Asp Ala Val Ala Glu Val Ala Leu Val Gly Glu 145 150 155 160 Glu Leu Val Ile Arg Gln Ala Ile Asn Glu Ala Leu Asp Gly Leu Glu 165 170 175 Ser Gln Thr Ser Val Val Lys Asn Thr Thr Ile Gly Asn Glu Thr Phe 180 185 190 Tyr Glu Ile Phe Gly Pro Asn Ser Ser Lys Glu Leu Ser Ile Lys His 195 200 205 Leu Phe Glu Tyr Phe Lys Val Ala Pro Glu Glu Thr Met Tyr Leu Gly 210 215 220 Asp Ser Phe Asn Asp Ile Gly Leu Leu Lys Met Val Gly Tyr Pro Ile 225 230 235 240 Val Met Glu Asn Gly His Asp Glu Val Lys Gln Tyr Ala Arg Tyr Ile 245 250 255 Thr Lys Ser Asn Asn Glu Ala Gly Val Ala Tyr Ala Val Lys Lys Trp 260 265 270 Ala Leu Gly Ile Asp Asp 275 <210> 66 <211> 270 <212> PRT <213> Fervidobacterium nodosum <400> 66 Met Val Arg Lys Thr Lys Thr Phe Val Phe Asp Leu Asp Gly Thr Val 1 5 10 15 Leu Thr Pro Lys Asn Glu Phe Pro Leu Glu Thr Lys Glu Leu Ile Met 20 25 30 Asn Ile Leu Lys Lys Gly Asp Asn Val Val Phe Ala Thr Gly Arg Met 35 40 45 His Ile Ser Ala Lys Lys Leu Leu Asp Arg Val Phe Gly Glu Asp Val 50 55 60 Phe Pro Ile Ile Ser Tyr Asn Gly Ala Val Val Tyr Val Pro Asp Lys 65 70 75 80 Gly Phe Ile Phe Glu Lys Thr Leu Glu Leu Ser Thr Ala His Lys Val 85 90 95 Ile Asp Phe Phe Arg Arg Asn Asn Ile His Val Gln Thr Tyr Val Glu 100 105 110 Asp Ile Leu Tyr Thr Glu Lys Asp Asn Glu Glu Ile Lys Leu Tyr Ser 115 120 125 Lys His Ala Glu Val Pro Tyr Thr Val Val Asp Asp Leu Lys Ala Ile 130 135 140 His Gln Pro Pro Ile Lys Ile Leu Gly Ile Gly Asp Gln Glu Leu Leu 145 150 155 160 Asp Arg Leu Ile Pro Asp Leu Lys Asn Val Val Gly Asn Ser Ala Thr 165 170 175 Val Phe Lys Ser Phe Ser Ile Phe Leu Asp Val Val Pro Ala Asp Ala 180 185 190 Asn Lys Gly Ile Ala Leu Arg Phe Leu Ala Asp Tyr Leu Lys Phe Asp 195 200 205 Leu Ser Glu Thr Ile Val Phe Gly Asp Asn Glu Asn Asp Ile Phe Met 210 215 220 Phe Glu Val Ala Gly Arg Lys Ile Ala Val Ser Asn Ala Val Glu Lys 225 230 235 240 Leu Lys Glu Val Ala Asp Phe Val Ser Lys Ser Asn Glu Glu Asn Gly 245 250 255 Val Tyr Phe Ala Phe Thr Gln Leu Phe Pro Glu Tyr Ile Val 260 265 270 <210> 67 <211> 276 <212> PRT <213> Fervidobacterium nodosum <400> 67 Met Val Lys Leu Ile Val Thr Asp Leu Asp Gly Thr Leu Leu Asn Asp 1 5 10 15 Asp Lys His Ile Pro Asp Asp Asn Ile Ile Ala Leu Arg Glu Ala Met 20 25 30 Glu Lys Gly Val His Val Ser Ile Ala Thr Gly Arg Asn Phe Gly Ser 35 40 45 Ala Lys Arg Tyr Ile Lys Glu Leu Gly Leu Asp Val Pro Val Ile Phe 50 55 60 Gln Asn Gly Ala Phe Ile Tyr Gln Trp Met Glu Asp Lys Val Ile Tyr 65 70 75 80 Lys Ser Asp Leu Lys Ser Glu Ile Ala Lys Leu Ile Val Glu Lys Ala 85 90 95 Arg Glu Lys Gly Leu Phe Tyr Val Val Tyr Ile Asp Phe Leu Glu Glu 100 105 110 Lys Asp Met Tyr Ile Asp Ala Asn Tyr Ser Gly Glu Phe Leu Ser Tyr 115 120 125 Leu Lys Gln Asn Glu Trp Arg Ile Asn Tyr Val Ser Asp Val Val Asn 130 135 140 Tyr Ile Ser Asn Arg Asp Ser Ile Ala Glu Val Ala Leu Val Gly Asp 145 150 155 160 Glu Glu Lys Ile Lys Asn Ile Val Glu Asp Asp Leu Phe Ile Phe Gly 165 170 175 Glu Ser Val Ser Val Val Lys Asn Asn Arg Ile Asn Ser Glu Val Phe 180 185 190 Tyr Glu Phe Phe Gly Pro Asn Ser Ser Lys Asp Ile Ser Phe Asn Tyr 195 200 205 Leu Leu Lys Tyr Phe Asn Val Lys Pro Glu Glu Thr Met Tyr Leu Gly 210 215 220 Asp Asn Tyr Asn Asp Ile Gly Met Leu Lys Ile Val Gly Tyr Pro Val 225 230 235 240 Val Met Glu Asn Ala Pro Asp Glu Val Lys Lys Tyr Ala Lys Tyr Val 245 250 255 Ser Lys Ser Asn Asn Glu Ala Gly Val Ala Tyr Ala Val Arg Lys Leu 260 265 270 Val Leu Gly Tyr 275 <210> 68 <211> 281 <212> PRT <213> Fervidobacterium pennivorans <400> 68 Met Ser Asn Ile Arg Lys Leu Lys Asn Ala Lys Thr Phe Val Phe Asp 1 5 10 15 Leu Asp Gly Thr Val Leu Asn Ser Lys Asn Glu Phe Pro Gln Glu Thr 20 25 30 Lys Gln Leu Ile Leu Asn Ile Leu Glu Asn Gly Asp Asn Val Ile Phe 35 40 45 Ala Thr Gly Arg Met His Ile Ser Ala Lys Lys Leu Leu Glu Asn Val 50 55 60 Phe Gly Glu Asp Ile Phe Pro Ile Ile Ser Tyr Asn Gly Ala Val Val 65 70 75 80 Tyr Val Pro Asn Val Gly Phe Ile Tyr Glu Lys Thr Leu Asp Met Glu 85 90 95 Thr Val His Lys Val Ile Gly Phe Leu Arg Gln Arg Asn Ile His Val 100 105 110 Gln Thr Tyr Val Asp Asp Lys Leu Tyr Ser Glu Lys Asp Asn Glu Glu 115 120 125 Ile Lys Leu Tyr Ala Lys His Ala Asp Val Pro Tyr Tyr Val Val Asn 130 135 140 Asp Leu Lys Ala Ile Glu His Pro Pro Ile Lys Met Leu Ala Ile Ala 145 150 155 160 Glu Gln Gly Ile Leu Asp Asn Leu Ile Glu Pro Leu Lys Glu Ile Val 165 170 175 Asp Gly Arg Ala Asn Val Phe Lys Ser Phe Pro Ile Phe Leu Asp Ile 180 185 190 Val Pro Ala Asp Ala Asn Lys Gly Asn Ala Leu Lys Phe Leu Ala Asp 195 200 205 Tyr Leu Gly Phe Asp Leu Lys Asn Thr Val Val Phe Gly Asp Asn Glu 210 215 220 Asn Asp Ile Tyr Met Phe Glu Val Ala Gly Thr Lys Val Ala Val Glu 225 230 235 240 Asn Ala Val Asp Lys Leu Lys Glu Val Ala Asp Phe Val Ser Lys Ser 245 250 255 Asn Glu Glu Asn Gly Val Leu Tyr Ser Phe Val Arg Leu Phe Pro Glu 260 265 270 Tyr Leu Arg Gly Ile Glu Ser Ser Asn 275 280 <210> 69 <211> 278 <212> PRT <213> Fervidobacterium pennivorans <400> 69 Met Val Lys Leu Ile Val Thr Asp Leu Asp Gly Thr Leu Leu Asn Asp 1 5 10 15 Glu Lys His Ile Pro Arg Glu Asn Ile Glu Ala Leu Lys Ala Ala Met 20 25 30 Asp Lys Gly Ile His Val Ser Val Ala Thr Gly Arg Asn Phe Tyr Ser 35 40 45 Ala Lys Pro Tyr Val Glu Lys Leu Gly Leu Asp Val Pro Val Ile Phe 50 55 60 Gln Asn Gly Ala Phe Ile Tyr Gln Trp Met Glu Asp Lys Val Ile Tyr 65 70 75 80 Lys Ser Glu Leu Lys Thr Glu Ile Ala Glu Arg Val Ile Glu Thr Ala 85 90 95 Arg Lys Tyr Lys Ile Phe Tyr Ile Leu Tyr Arg Asp Phe Leu Glu Glu 100 105 110 Lys Asp Met Tyr Ile Asp Gln Pro Tyr Ser Gly Asn Phe Ser Leu Tyr 115 120 125 Leu Asn Gln Asn Arg Trp Arg Leu Asn Ile Val Asp Asp Val Leu Lys 130 135 140 Tyr Leu Lys Asn Lys Leu Thr Val Ala Glu Val Ala Leu Val Gly Asn 145 150 155 160 Glu Glu Arg Ile Met Leu Ala Leu Asn Glu Ser Leu Gly Glu Val Arg 165 170 175 Asn Ala Thr Ser Ile Val Lys Asn Asn Thr Ile Asn Glu Glu Thr Phe 180 185 190 Tyr Glu Val Phe Gly Pro Asn Ala Ser Lys Glu Leu Ser Ile Lys Tyr 195 200 205 Leu Phe Glu Tyr Phe Asn Val Ala Pro Glu Glu Thr Met Tyr Leu Gly 210 215 220 Asp Ser Phe Asn Asp Ile Gly Leu Leu Lys Met Val Gly Tyr Pro Val 225 230 235 240 Val Met Glu Asn Gly His Ser Glu Val Lys Gln Tyr Ala Arg Tyr Ile 245 250 255 Thr Lys Ser Asn Asn Glu Ala Gly Val Ala Tyr Ala Val Arg Lys Trp 260 265 270 Ala Leu Gly Ile Asp Glu 275 <210> 70 <211> 215 <212> PRT <213> Fervidobacterium pennivorans <400> 70 Met Lys Pro Lys Ala Cys Ile Phe Asp Leu Asp Gly Val Ile Val Asp 1 5 10 15 Thr Ala Lys Tyr His Tyr Leu Ala Trp Lys Arg Leu Ala Lys Glu Leu 20 25 30 Gly Phe Glu Phe Thr Glu Lys Asp Asn Glu Arg Leu Lys Gly Val Ser 35 40 45 Arg Met Glu Ser Leu Glu Ile Leu Leu Ser Val Gly Gly Ile Lys Ile 50 55 60 Asp Asp Glu Asn Val Lys Leu Gln Leu Ala Glu Lys Lys Asn Lys Trp 65 70 75 80 Tyr Val Glu Tyr Ile Asn Gln Met Thr Lys Asp Glu Ile Leu Pro Gly 85 90 95 Val Ile Glu Phe Leu Glu Leu Leu Lys Ser Ala Gly Ile Lys Val Ala 100 105 110 Ile Gly Ser Ala Ser Lys Asn Thr Ile Thr Ile Leu Glu Arg Ile Gly 115 120 125 Leu Lys Asp Phe Phe Asp Val Ile Ile Asp Gly Thr Lys Ile Ser Lys 130 135 140 Ala Lys Pro Asp Pro Glu Val Phe Leu Lys Ala Ala Glu Glu Leu Ser 145 150 155 160 Val Arg Pro Glu Glu Cys Cys Val Phe Glu Asp Ala Val Ala Gly Ile 165 170 175 Gln Ala Ala Lys Ser Ala Gly Met Lys Val Ile Gly Val Gly Asp Pro 180 185 190 Thr Ile Leu Lys Asp Ala Asp Lys Val Ile Gln Ser Phe Gln Gly Gln 195 200 205 Thr Leu Gly Leu Ile Glu Phe 210 215 <210> 71 <211> 222 <212> PRT <213> Geobacillus sp. <400> 71 Met Thr Ile Arg Thr Ile Leu Phe Asp Leu Asp Gly Thr Leu Ile Asp 1 5 10 15 Thr Asn Glu Leu Ile Ile Gln Ser Phe Leu His Thr Leu Glu Lys Tyr 20 25 30 Tyr Pro Gly Arg Tyr Gly Arg Glu Asp Val Leu Pro Phe Ile Gly Pro 35 40 45 Ser Leu Tyr Glu Thr Phe Ser Ser Leu Asp Pro Glu Arg Ala Glu Glu 50 55 60 Met Val Lys Thr Tyr Arg Thr Phe Asn His Ala Arg His Asp Glu Leu 65 70 75 80 Ile Arg Glu Phe Asp Thr Val Tyr Glu Thr Ile Glu Thr Leu His Arg 85 90 95 His Gly Phe Arg Leu Gly Val Val Thr Thr Lys Met His Asp Thr Ala 100 105 110 Leu Met Gly Leu Arg Lys Thr Arg Leu Glu Pro Phe Phe Ser Cys Val 115 120 125 Ile Gly Leu Asp Asp Val Ser Arg Pro Lys Pro Asp Pro Glu Pro Ile 130 135 140 His Lys Ala Leu Glu Ala Leu Gln Ser Lys Ser Asp Glu Ala Leu Met 145 150 155 160 Val Gly Asp Asn Tyr His Asp Ile Leu Ala Gly Lys Asn Ala Gly Val 165 170 175 Lys Thr Ala Gly Val Ala Trp Ala Ile Lys Gly Arg Gly Tyr Leu Glu 180 185 190 Gln Tyr Glu Pro Asp Tyr Met Leu Glu Lys Met Ser Asp Leu Leu Ala 195 200 205 Ile Val Gly Ile Asn Glu Arg Lys Glu Glu Thr Ser Ser Gln 210 215 220 <210> 72 <211> 264 <212> PRT <213> Geobacillus stearothermophilus <400> 72 Met Ala Glu Lys Trp Glu Glu Ile Asp Arg Tyr Ala Arg Gln Trp Ile 1 5 10 15 Asp Glu Ala Gly Lys Arg Ile Arg Ala Ser Phe Ala Lys Gln Leu Thr 20 25 30 Val Glu Ala Lys Glu Asn Pro Asn Asp Leu Val Thr Asn Val Asp Arg 35 40 45 Ala Ile Glu Gln Phe Phe Ala Glu His Ile Arg Arg Gln Phe Pro Ser 50 55 60 His Arg Leu Leu Gly Glu Glu Gly Phe Gly Asp Arg Ile Asp Ala Leu 65 70 75 80 Asp Gly Val Val Trp Val Ile Asp Pro Ile Asp Gly Thr Met Asn Phe 85 90 95 Val His Gln Arg Arg His Phe Ala Val Ser Ile Gly Ile Phe Glu Asp 100 105 110 Gly Ile Gly Gln Leu Gly Tyr Val Tyr Asp Val Val Phe Asp Glu Leu 115 120 125 Tyr Ala Ala Gln Lys Gly Arg Gly Val Phe Leu Asn Gly Glu Pro Leu 130 135 140 Gly Leu Leu Gln Pro Ala Pro Val Ala Glu Ser Ile Ile Ala Ile Asn 145 150 155 160 Gly Thr Trp Leu Met Glu Asn Lys Arg Leu Asp His Arg Pro Leu Met 165 170 175 Arg Leu Ala Lys Glu Ala Arg Gly Thr Arg Ser Tyr Gly Ser Ala Ala 180 185 190 Leu Glu Leu Ala Tyr Val Ala Ala Gly Arg Leu Asp Ala Tyr Ile Ser 195 200 205 Pro Arg Leu Ser Pro Trp Asp Phe Ala Gly Gly Met Ile Leu Ile Glu 210 215 220 Glu Ala Gly Gly Met Val Thr Thr Leu Asp Gly Lys Pro Leu Asp Leu 225 230 235 240 Leu Gly Arg Asn Ser Val Leu Ala Ala Lys Pro Gly Val His Glu Glu 245 250 255 Ile Leu Arg Arg Tyr Leu His Asp 260 <210> 73 <211> 283 <212> PRT <213> Geobacillus stearothermophilus <400> 73 Met Leu Ile Ala Leu Asp Met Asp Gly Thr Leu Leu Asn Gly Glu Gly 1 5 10 15 Lys Ile Ser Glu Arg Asn Arg Lys Ala Met Met Ala Ala Gln Lys Glu 20 25 30 Gly His Ile Val Ala Val Ile Thr Gly Arg Ala Arg Lys Asp Ala Leu 35 40 45 Ala Pro Leu Arg Glu Ala Gly Leu Val Cys Pro Ile Ala Ser Leu Asn 50 55 60 Gly Ala Ile Val Thr Leu Glu Asp Gly Thr Val Ile Gly Glu Ala Pro 65 70 75 80 Leu Ala Arg Thr Ala Val Arg Pro Thr Leu Glu Trp Val Arg Lys Gln 85 90 95 Ser Asp Leu Tyr Cys Glu Thr Tyr Thr Gly Asp Ala Val Tyr Val Gly 100 105 110 Leu His Asn Arg Ala Gln Trp Glu Ser Leu Ala Ser Glu Met Ala Asp 115 120 125 Ala Ala Pro Asp Val Lys Trp Leu Val Asn Lys Gln Phe Gln Gln Ala 130 135 140 Arg Val Thr Tyr Val Asp Asp Ile Arg Thr Val Trp Asp Asp Pro Gln 145 150 155 160 Leu Thr Leu Tyr Lys Leu Leu Ile Phe Ala Leu Asp Arg Glu Arg Leu 165 170 175 Arg Asp Ala Ala Ser Arg Phe Ala Ala Leu Pro Ser Val Thr Val Thr 180 185 190 Ser Ser His Pro His Asn Ile Glu Met Asn Asn Glu Arg Ala Thr Lys 195 200 205 Gly Glu Ala Leu Lys Gln Leu Ala Ala His Tyr Gly Ile Asp Leu Cys 210 215 220 Asp Thr Val Ala Phe Gly Asp Ser His Asn Asp Leu Ser Met Phe Glu 225 230 235 240 Val Ala Gly Cys Arg Val Ala Met Ala Asn Ala Ala Pro Glu Leu Lys 245 250 255 Ala Lys Ala Asp Ile Val Thr Cys Ser His Glu Glu Asp Gly Val Ala 260 265 270 Ala Gly Leu Glu Arg Val Leu Ala Lys Arg Val 275 280 <210> 74 <211> 268 <212> PRT <213> Geobacillus stearothermophilus <400> 74 Met Lys Pro Tyr Leu Ile Ala Leu Asp Leu Asp Gly Thr Leu Leu Lys 1 5 10 15 Glu Asp Lys Thr Ile Ser Pro Phe Thr Lys Asp Val Ile Arg Arg Ala 20 25 30 Ile Asp Thr Gly His Phe Val Val Ile Ala Thr Gly Arg Pro Tyr Arg 35 40 45 Ala Ser Ser Met Tyr Tyr Glu Glu Leu Gly Leu Ala Thr Pro Ile Val 50 55 60 Asn Phe Asn Gly Ala Phe Val His His Pro Arg Gln Pro Ser Trp Gly 65 70 75 80 Met His His Tyr Pro Leu Pro Leu Ala Val Val Lys Asp Ile Val Glu 85 90 95 Ile Ser Glu Ser Tyr Gly Ile Lys Asn Met Met Ala Glu Val Leu Asp 100 105 110 Asn Val Tyr Phe His Gln His Asp Glu Val Leu Leu Asp Ile Val Arg 115 120 125 Leu Gly Asn Pro Thr Val Glu Ile Gly Asp Leu Arg Arg Ser Leu Gly 130 135 140 Lys Asp Pro Thr Ser Val Leu Val Tyr Thr Asp Asp Asp His Ile Glu 145 150 155 160 Arg Ile Gln Ser His Leu Ala Asn Val Tyr Ala Asn Val Ile His Gln 165 170 175 Arg Arg Trp Ser Glu Pro Trp His Val Ile Glu Ile Ile Arg His Gly 180 185 190 Val His Lys Ala Ala Gly Leu Lys Gln Val Ala Asp Tyr Phe Gly Ile 195 200 205 Pro Arg Glu Arg Val Ile Ala Phe Gly Asp Glu Asp Asn Asp Leu Glu 210 215 220 Met Ile Asp Trp Ala Gly Leu Gly Val Ala Met Gly Asn Ala Ile Glu 225 230 235 240 Pro Leu Lys Thr Ile Ala Asp Asp Val Ala Lys Thr Asn Glu Glu Asp 245 250 255 Gly Val Gly Val Tyr Leu Gln Asp Leu Leu Arg Leu 260 265 <210> 75 <211> 287 <212> PRT <213> Geobacillus stearothermophilus <400> 75 Met Tyr Lys Leu Leu Ala Leu Asn Ile Asp Gly Thr Ile Leu Lys Asn 1 5 10 15 Asn Gly Arg Leu Pro Arg Glu Thr Lys Glu Ala Val Asp Tyr Val Lys 20 25 30 Lys Lys Gly Val Tyr Val Thr Leu Ile Thr Ser Arg Asn Leu Leu Ser 35 40 45 Ala Arg Lys Val Ala Lys Ala Leu Arg Leu Asp Gly Met Leu Ile Ala 50 55 60 Phe Gln Gly Ala Met Ile Ala Arg Thr Leu Asp Asp Arg Leu Phe Asp 65 70 75 80 Ala Val Ile Pro Glu Glu Arg Thr Phe Asn Ile Val Gln Ile Leu Glu 85 90 95 Asn Phe Asn Cys Asn Ile Arg Leu Met His Glu Arg Tyr Ser Leu Gly 100 105 110 Asn Arg Lys Lys Val Lys Lys Asn Leu Val Val Gln Thr Val Leu Ser 115 120 125 Ser Ser Asp Pro Phe Phe Tyr Pro Thr Gln Phe Val Asp Ser Leu Gly 130 135 140 Asp Val Leu Met Asp Glu Pro Ile Ala Val Pro Lys Ile Asp Val Tyr 145 150 155 160 Phe Ala Thr Asp Glu Glu Arg Asp Ala Ala Ala Ala Leu Leu Thr Lys 165 170 175 Ser Ile Pro Ser Ile Asp Met Ile Met Gln Pro Asn Gly Lys Met Glu 180 185 190 Ile Val Pro Gln Gly Val Ser Lys Leu Ala Gly Leu Arg Arg Leu Val 195 200 205 Gln His Ile Gly Val Ser Leu Lys Glu Thr Val Met Ile Gly Asp Gly 210 215 220 Leu Asp Asp Leu Pro Ala Ile Glu Ala Ala Gly Leu Gly Val Ala Met 225 230 235 240 Gly Asn Ala Pro Leu Glu Val Lys Arg Ala Ala Asp Trp Val Thr Arg 245 250 255 Ser Asn Glu Gln Leu Gly Val Ala Tyr Met Val Lys Glu His Phe Arg 260 265 270 Lys Gln Gln Arg Ile Glu Phe Leu Gln Lys Leu Lys Thr Lys Gln 275 280 285 <210> 76 <211> 258 <212> PRT <213> Geobacillus stearothermophilus <400> 76 Met Gly Arg Lys Ile Val Phe Phe Asp Ile Asp Gly Thr Leu Leu Asp 1 5 10 15 Glu Gln Lys Gln Leu Pro Leu Ser Thr Ile Glu Ala Val Arg Arg Leu 20 25 30 Lys Gln Ser Gly Val Tyr Val Ala Ile Ala Thr Gly Arg Ala Pro Phe 35 40 45 Met Phe Glu His Val Arg Lys Gln Leu Gly Ile Asp Ser Phe Val Ser 50 55 60 Phe Asn Gly Gln Tyr Val Val Phe Glu Gly Asn Val Leu Tyr Lys Gln 65 70 75 80 Pro Leu Arg Arg Glu Lys Val Arg Ala Leu Thr Glu Glu Ala His Lys 85 90 95 Asn Gly His Pro Leu Val Phe Met Asp Ala Glu Lys Met Arg Ala Ser 100 105 110 Ile Ser Asp His Pro His Ile His Val Ser Met Ala Ser Leu Lys Phe 115 120 125 Ala His Pro Pro Val Asp Pro Leu Tyr Tyr Glu Asn Lys Asp Ile Tyr 130 135 140 Gln Ala Leu Leu Phe Cys Arg Ala Glu Glu Glu Glu Pro Tyr Val Arg 145 150 155 160 Asn Tyr Pro Glu Phe Arg Phe Val Arg Trp His Asp Val Ser Thr Asp 165 170 175 Val Leu Pro Ala Gly Gly Ser Lys Ala Glu Gly Ile Arg Met Met Ile 180 185 190 Glu Lys Leu Gly Ile Asp Lys Lys Asp Val Tyr Ala Phe Gly Asp Gly 195 200 205 Leu Asn Asp Ile Glu Met Leu Ser Phe Val Gly Thr Gly Val Ala Met 210 215 220 Gly Asn Ala His Glu Glu Val Lys Arg Val Ala Asp Phe Val Thr Lys 225 230 235 240 Pro Val Asp Lys Glu Gly Ile Trp Tyr Gly Leu Lys Gln Leu Gln Leu 245 250 255 Ile Arg <210> 77 <211> 237 <212> PRT <213> Halococcus salifodinae <400> 77 Met Asn Arg Ala Asp Ala Ala Pro Glu Ala Arg Pro Pro Leu Ala Val 1 5 10 15 Asp Ile Asp Gly Thr Leu Thr Asp Arg Asn Arg Val Val Asp Pro Arg 20 25 30 Val Phe Asp Ala Leu Arg Glu Trp Pro Ala Pro Val Val Leu Ala Thr 35 40 45 Gly Lys Ala Leu Pro Tyr Pro Val Ala Leu Cys Glu Phe Ala Gly Leu 50 55 60 Ala Thr Leu Val Val Ala Glu Asn Gly Gly Val Val Cys Leu Asp Thr 65 70 75 80 Glu Thr Arg Asp Asp Ile Val Val Asp Gly Asp Arg Thr Ala Ala Asp 85 90 95 Arg Val Ala Ala Ala Tyr Arg Asp Ala Gly His Asp Leu Gly Trp Gly 100 105 110 Ser Leu Asp Leu Val Asn Arg Trp Arg Glu Thr Glu Ile Ala Val Ser 115 120 125 Arg Asp Ala Pro Leu Asp Pro Leu Arg Glu Ile Ala Val Glu Tyr Gly 130 135 140 Leu Asp Val Val Asp Thr Gly Phe Ala Tyr His Val Val Ser Pro Asp 145 150 155 160 Val Asp Lys Gly Arg Gly Leu Ser Ile Val Ala Asp Arg Leu Gly Arg 165 170 175 Asp Pro Glu Glu Phe Val Ala Ile Gly Asp Ser Ala Asn Asp Val Ala 180 185 190 Thr Phe Glu Val Val Gly Arg Ser Phe Ala Val Ala Asn Ala Asp Glu 195 200 205 Ala Ala Thr Gln Ala Ala Asp Leu Val Thr Asp Ala Ala Tyr Ala Asp 210 215 220 Gly Phe Ile Glu Ala Leu Ser Thr Val Arg Ser Ser Ser 225 230 235 <210> 78 <211> 237 <212> PRT <213> Halococcus salifodinae <400> 78 Met Asn Arg Ala Asp Ala Ala Pro Glu Ala Arg Pro Pro Leu Ala Val 1 5 10 15 Asp Ile Asp Gly Thr Leu Thr Asp Arg Asn Arg Val Val Asp Pro Arg 20 25 30 Val Phe Asp Ala Leu Arg Glu Trp Pro Ala Pro Val Val Leu Ala Thr 35 40 45 Gly Lys Ala Leu Pro Tyr Pro Val Ala Leu Cys Glu Phe Ala Gly Leu 50 55 60 Ala Thr Leu Val Val Ala Glu Asn Gly Gly Val Val Cys Leu Asp Thr 65 70 75 80 Glu Thr Arg Asp Asp Ile Val Val Asp Gly Asp Arg Thr Ala Ala Asp 85 90 95 Arg Val Ala Ala Ala Tyr Arg Asp Ala Gly His Asp Leu Gly Trp Gly 100 105 110 Ser Leu Asp Leu Val Asn Arg Trp Arg Glu Thr Glu Ile Ala Val Ser 115 120 125 Arg Asp Ala Pro Leu Asp Pro Leu Arg Glu Ile Ala Val Glu Tyr Gly 130 135 140 Leu Asp Val Val Asp Thr Gly Phe Ala Tyr His Val Val Ser Pro Asp 145 150 155 160 Val Asp Lys Gly Arg Gly Leu Ser Ile Val Ala Asp Arg Leu Gly Arg 165 170 175 Asp Pro Glu Glu Phe Val Ala Ile Gly Asp Ser Ala Asn Asp Val Ala 180 185 190 Thr Phe Glu Val Val Gly Arg Ser Phe Ala Val Ala Asn Ala Asp Glu 195 200 205 Ala Ala Thr Gln Ala Ala Asp Leu Val Thr Asp Ala Ala Tyr Ala Asp 210 215 220 Gly Phe Ile Glu Ala Leu Ser Thr Val Arg Ser Ser Ser 225 230 235 <210> 79 <211> 183 <212> PRT <213> Halococcus salifodinae <400> 79 Met Thr Val Thr Ile Pro Asp Asp Thr Glu Ala Val Val Tyr Asp Leu 1 5 10 15 Asp Gly Thr Leu Val Arg Leu Ala Val Asp Trp Asp Ala Ala Ala Thr 20 25 30 Asp Ala Ala Ala Ala Leu Asp Gly Cys Gly Val Asp Thr Asp Gly Met 35 40 45 Asp Leu Trp Arg Met Leu Glu Arg Ala Asp Glu Leu Gly His Arg Asp 50 55 60 Ala Val Glu Glu Ala Ile Ala Gly His Glu Arg Glu Gly Ala Arg Arg 65 70 75 80 Ser Glu Arg Leu Ala Phe Ala Asp Glu Leu Val Thr Asp Arg Pro Val 85 90 95 Gly Val Cys Ser Leu Asn Cys Glu Asp Ala Cys Arg Ile Ala Leu Asp 100 105 110 Val His Asp Leu Ala Glu His Val Ala Cys Val Val Gly Arg Asp Thr 115 120 125 Val Ala Thr Phe Lys Pro Asp Pro Glu Ser Leu Leu Ala Ala Val Glu 130 135 140 His Leu Asp Ala Thr Pro Glu Arg Thr Leu Phe Ile Gly Asp Ser Glu 145 150 155 160 Gly Asp Ala Glu Thr Ala Asp Arg Ala Gly Thr Arg Phe Ser Tyr Val 165 170 175 Gly Asp Gly Pro Thr Thr Tyr 180 <210> 80 <211> 212 <212> PRT <213> Hydrogenivirga sp. 128-5-R1-1 <400> 80 Met Lys Ala Val Leu Phe Asp Leu Asp Gly Thr Leu Ile Asp Ser Ala 1 5 10 15 Asp Asp Ile Ala Leu Ala Leu Arg Leu Thr Leu Glu Glu Leu Gly Met 20 25 30 Glu Glu Lys Met Pro Pro Ser Val Arg Ser Leu Ile Gly Gly Gly Val 35 40 45 Lys Ala Leu Leu Glu Arg Val Leu Gly Asp Asp Phe Arg Glu Gln His 50 55 60 Val Lys Val Phe Arg Arg His Tyr Ile Gly Asn Pro Val Val Asn Thr 65 70 75 80 Thr Pro Tyr Pro Gly Val Met Glu Thr Leu Arg Ala Leu Arg Gly Arg 85 90 95 Gly Ile Asn Leu Val Val Val Thr Asn Lys Leu Glu Glu Leu Ser Val 100 105 110 Glu Ile Leu Lys Arg Leu Gly Met Leu Glu Phe Phe Asp Leu Val Val 115 120 125 Gly Gly Asp Thr Phe Pro Glu Lys Lys Pro Ser Pro Leu Pro Val Leu 130 135 140 Lys Ser Leu Glu Phe Val Gly Val Glu Ser Ser Gln Ala Leu Met Val 145 150 155 160 Gly Asp Thr Ser Ala Asp Ile Glu Ala Gly Lys Arg Ala Gly Val Arg 165 170 175 Thr Ala Leu Ala Ala Trp Gly Tyr Val Lys Leu Asn Ser Val Lys Pro 180 185 190 Asp Tyr Ile Phe Asn Ser Pro Ala Asp Ile Leu Ser Leu Ala Ser Gln 195 200 205 Val Ser Gly Ala 210 <210> 81 <211> 211 <212> PRT <213> Hydrogenobacter hydrogenophilus <400> 81 Met Arg Leu Lys Leu Phe Ile Phe Asp Leu Asp Gly Thr Leu Ile Asp 1 5 10 15 Ser Tyr Met Asp Ile Gly Met Cys Val Asn Met Val Leu Gln Glu Met 20 25 30 Gly Arg Asn Pro Ile Asp Pro Glu Arg Val Lys Ala Trp Ile Gly Gly 35 40 45 Gly Ala Arg Arg Leu Leu Glu Lys Leu Phe Pro Asp Glu Glu Leu Asn 50 55 60 Thr Ala Leu Glu Leu Phe Arg Arg Phe Tyr Arg Glu Asn Pro Val Val 65 70 75 80 His Thr Lys Thr Tyr Glu Gly Ile Gln Glu Val Leu Ala Tyr Leu Arg 85 90 95 Ser Arg Gly Ser Phe Leu Ala Val Val Thr Asn Lys Met Glu Asp Leu 100 105 110 Ser Arg Glu Ile Leu Arg Arg Leu Glu Leu Leu Ser Tyr Phe Asp Val 115 120 125 Val Val Gly Gly Asp Thr Phe Pro Glu Lys Lys Pro Ser Pro Leu Pro 130 135 140 Ile Lys Gln Val Met Glu Arg Leu Arg Val Arg Pro Gln Glu Ser Val 145 150 155 160 Ile Val Gly Asp Thr Ser Ala Asp Ile Cys Ala Gly Lys Asp Ala Gly 165 170 175 Ile Trp Thr Ala Leu Ala Lys Trp Gly Tyr Val Arg Leu Asp Ser Val 180 185 190 Met Pro Asp Phe Phe Leu Ser Glu Pro Lys Asp Val Ile Asp Leu Phe 195 200 205 Glu Lys Val 210 <210> 82 <211> 262 <212> PRT <213> Hydrogenobacter hydrogenophilus <400> 82 Met Asp Lys Val Glu Ser Phe Leu Lys Val Ala Lys Glu Ser Ala Leu 1 5 10 15 Ile Gly Gly Leu Ile Leu Lys Glu His Phe Gly Lys Leu Thr Gln Lys 20 25 30 Asp Val Gln Glu Lys Ser Glu Lys Asp Val Val Ser Phe Val Asp Lys 35 40 45 Ser Ser Glu Glu Arg Ile Arg Lys Tyr Ile Lys Ile Asn Phe Pro Asp 50 55 60 His Asp Val Val Gly Glu Glu Glu Gly Gly Thr Asp Asn Ser Asp Tyr 65 70 75 80 Val Trp Tyr Ile Asp Pro Leu Asp Gly Thr Lys Asn Tyr Ile Ala Gly 85 90 95 Phe Pro Ile Phe Gly Val Ser Val Gly Leu Thr Tyr Arg Lys Glu Pro 100 105 110 Ile Val Gly Ala Val Tyr Met Pro Leu Tyr Asp Thr Leu Tyr Trp Ala 115 120 125 Tyr Lys Gly Gly Gly Ala Tyr Lys Asn Gly Lys Ser Ile Glu Val Ser 130 135 140 Lys Arg Val Gln Val Lys Ser Phe Phe Ile Ala Tyr Gly Phe Pro Ser 145 150 155 160 Arg Ala Lys Arg Asn Leu Asp Ile Tyr Trp Asn Ile Phe Lys Glu Leu 165 170 175 Phe Glu Lys Val Gly Ala Met Arg Arg Pro Gly Ala Ala Ala Ile Asp 180 185 190 Leu Cys Phe Thr Ala Glu Gly Ile Phe Asp Gly Leu Ile Glu Phe Glu 195 200 205 Leu Asn Pro Trp Asp Val Cys Ala Gly Ile Leu Ile Leu Gln Glu Ala 210 215 220 Gly Gly Lys Val His Leu Thr Lys Asp Leu Thr Lys Gly Thr Asp Val 225 230 235 240 Ile Ala Gly Thr Pro Thr Ala Phe Pro Tyr Ile Glu Asn Thr Val Lys 245 250 255 Ser Asn Leu Glu Gly Leu 260 <210> 83 <211> 211 <212> PRT <213> Hydrogenobacter thermophilus <400> 83 Met Arg Leu Lys Leu Phe Ile Phe Asp Leu Asp Gly Thr Leu Ile Asp 1 5 10 15 Ser Tyr Met Asp Ile Gly Met Cys Val Asn Gly Val Leu Arg Ser Met 20 25 30 Gly Arg Gln Glu Ile Glu Pro Asp Ser Val Lys Asn Trp Ile Gly Gly 35 40 45 Gly Ala Arg Gly Leu Leu Glu Lys Leu Phe Pro Ser Glu Glu Ile Asp 50 55 60 Arg Ala Leu Glu Leu Phe Arg Lys Tyr Tyr Arg Glu Asn Pro Val Val 65 70 75 80 Tyr Thr Lys Ala Tyr Lys Gly Ile Arg Glu Val Leu Thr His Leu Lys 85 90 95 Ala Met Glu Lys Arg Leu Ala Val Val Thr Asn Lys Met Glu Asp Leu 100 105 110 Ser Val Glu Ile Leu Lys Arg Leu Asn Leu Tyr His Tyr Phe Asp Met 115 120 125 Val Val Gly Gly Asp Thr Phe Pro Glu Lys Lys Pro Ser Pro Val Pro 130 135 140 Ile Lys Glu Val Leu Arg Trp Leu Gly Val Asp Ala Lys Glu Ala Val 145 150 155 160 Met Ile Gly Asp Thr Asp Ala Asp Val Arg Ala Gly Lys Asp Ala Gly 165 170 175 Val Trp Thr Ala Leu Ala Asn Trp Gly Tyr Val Arg Met Asp Gly Val 180 185 190 Lys Pro Asp Phe Val Leu His Lys Pro Glu Asp Ile Ile Asn Leu Pro 195 200 205 Gly Arg Ala 210 <210> 84 <211> 247 <212> PRT <213> Hyperthermus butylicus <400> 84 Met Ala Glu Ala Val Arg Ile Glu Leu Pro Ala Arg Val Cys Gly Leu 1 5 10 15 Ala Leu Asp Ile Asp Gly Thr Leu Thr Glu Lys Lys Arg Leu Gly Glu 20 25 30 Phe Asn Ile Ser Leu Glu Ala Val Glu Ala Val Arg Arg Ala Glu Ala 35 40 45 Ala Gly Ile Pro Val Met Leu Val Thr Gly Asn Ser Val Tyr Val Val 50 55 60 Ala Gly Val Ala Arg Tyr Ile Gly Ala Ser Gly Pro His Val Ala Glu 65 70 75 80 Asn Gly Cys Ile Val Tyr Asp Asn Gly Thr Ile Tyr Arg Val Cys Arg 85 90 95 Asp Thr Ala Arg Arg Ala Ala Arg Leu Ile Glu Glu Glu Leu Ala Gly 100 105 110 Val Leu Lys Pro Ser Trp Gln Asn Pro Cys Arg Leu His Asp Tyr Ala 115 120 125 Phe Ile Pro Lys Leu Leu Glu Pro Gly Glu Ala Leu Glu Ala Val Lys 130 135 140 Arg Val Leu Ser Ser Arg Gly Ile Asn Val Lys Val Gly Phe Ser Gly 145 150 155 160 Tyr Ala Ile His Leu Arg Pro Val Asp Ala Ser Lys Gly Arg Gly Leu 165 170 175 Lys Leu Ala Leu Lys Leu Arg Gly Leu Glu Pro Asp Cys Val Val Ala 180 185 190 Val Gly Asp Ser Val Ile Asp Leu Glu Met Lys Asp Ala Gly Val Ile 195 200 205 Leu Ala Ala Val Gly Asn Ala Asp Glu Lys Leu Lys Lys Asn Ala Asp 210 215 220 Ile Val Leu Pro Gly Glu Ser Gly Arg Ser Val Lys Ala Leu Ile Asp 225 230 235 240 Ala Ile Leu Ser Asn Arg Gly 245 <210> 85 <211> 267 <212> PRT <213> Kosmotoga arenicorallina <400> 85 Met Lys Met Ile Glu Ala Phe Val Ile Asp Ile Asp Gly Thr Phe Ile 1 5 10 15 Asp Ser Lys Gly Asn Ile Pro Gln Glu Asn Leu Asp Ala Leu Lys Glu 20 25 30 Ile Glu Glu Glu Gly Ile Met Ile Phe Phe Ala Ser Gly Arg Met Leu 35 40 45 Ser Ser Val Ile Lys Phe Gln Ser Ser Lys Leu Asn Lys Ile His Pro 50 55 60 Ile Ile Ala Tyr Asn Gly Ala Val Val Trp Asn Gly Arg Asp Val Ile 65 70 75 80 Phe Ser Lys Asn Ile Thr Thr Glu Met Ala Lys Asp Ile Val Ser Tyr 85 90 95 Ser Leu Ser Lys Gly Leu Tyr Ile Gln Ala Tyr Val Asn Asp Glu Leu 100 105 110 Val Val Pro Phe Asp Cys Glu Lys Ala Arg Glu Tyr Ala Lys His Ser 115 120 125 Ala Val Asp Phe Arg Val Glu Glu Lys Phe Ile Glu Phe Val Thr Lys 130 135 140 Asn Thr Pro Thr Lys Leu Leu Ile Ile Asp Thr Pro Glu Lys Val Glu 145 150 155 160 Val Leu Ser Ala His Phe Ala Ala Arg Tyr Asn His Leu Asn Val Phe 165 170 175 Arg Ser Phe Ala Thr Tyr Leu Asp Leu Leu Pro Glu Gly Val Asn Lys 180 185 190 Gly Leu Gly Leu Lys Tyr Leu Cys Glu Asp Leu His Val Asp Pro Ala 195 200 205 Cys Val Val Ala Phe Gly Asp Asn Asp Asn Asp Val Pro Leu Phe Glu 210 215 220 Glu Ala Gly Phe Ser Val Ala Val Ala Asn Ala Thr Pro Asn Ala Arg 225 230 235 240 Lys Ala Ala Asp Val Ile Ala Ser Ala Asn Tyr Glu Ala Gly Phe Gly 245 250 255 Arg Thr Ala Met Lys Leu Ile Lys Leu Leu Lys 260 265 <210> 86 <211> 265 <212> PRT <213> Kosmotoga olearia <400> 86 Met Ile Asn Gly Val Val Ile Asp Ile Asp Gly Thr Phe Ile Asp Ser 1 5 10 15 Glu Glu Lys Ile Pro Ile Glu Asn Leu Glu Val Phe Arg Glu Leu Glu 20 25 30 Ala Lys Gly Leu Arg Val Ile Phe Ala Ser Gly Arg Met Leu Thr Ser 35 40 45 Val Lys Asn Phe Ile Ser Lys Ile Ser Asp Lys Ala Tyr Pro Ile Ile 50 55 60 Ala Tyr Asn Gly Ala Val Val Tyr Val Asn Gly Glu Asn Ile Phe Asn 65 70 75 80 Gln Val Leu Leu Gln Asp Thr Ala Val Arg Ile Val Glu Arg Ala Leu 85 90 95 Ser Asn Asn Met Tyr Ile Gln Ala Tyr Val Asp Asp Arg Leu Val Val 100 105 110 Pro Lys Asp Cys Glu Glu Ala Arg Ser Tyr Ala Ser His Ser Gly Val 115 120 125 Asp Phe Met Val Val Glu Asp Leu Thr Asn Tyr Leu Ser Lys His Pro 130 135 140 Thr Ile Lys Leu Leu Met Ile Ala Pro Ser Glu Gln Ile Asp Asn Leu 145 150 155 160 Arg Leu Glu Phe Ser Glu Ile Phe Pro Glu Val Asp Phe Val Arg Ser 165 170 175 Phe Ser Thr Tyr Leu Asp Ile Val Pro Lys Gly Val Ser Lys Gly Lys 180 185 190 Ala Leu Glu Ile Leu Cys Lys His Leu Glu Ile Asp Ile Gly Lys Leu 195 200 205 Ile Ala Phe Gly Asp Asn Asp Asn Asp Ile Ser Leu Phe Glu Arg Cys 210 215 220 Gly Phe Ser Ile Ala Met Ala Asn Ala Thr Gln Arg Ala Lys Lys Ala 225 230 235 240 Ala Asp Val Ile Ala Pro Ser Asn Asp Glu Ala Gly Phe Ala Arg Val 245 250 255 Met Lys Lys Leu Leu Thr Leu Cys Asp 260 265 <210> 87 <211> 280 <212> PRT <213> Kosmotoga olearia <400> 87 Met Ile Lys Leu Ile Ala Ile Asp Leu Asp Gly Thr Leu Leu Asn Asn 1 5 10 15 Lys Lys Glu Ile Ser Leu Glu Asn Thr Ala Ala Leu Thr Glu Ala Met 20 25 30 Glu Lys Gly Leu His Val Ser Ile Phe Thr Gly Arg Ser Tyr Ile Ser 35 40 45 Gly Ser Glu Tyr Ala Lys Lys Leu Gly Leu Arg Val Pro Val Val Tyr 50 55 60 Gln Asn Gly Ala Leu Ile Ile Asn Ser Asp Asp Gly Asn Lys Gly Val 65 70 75 80 Ile Arg Lys Val Ile Leu Ser Ala Lys Arg Ala Arg Glu Ile Val Glu 85 90 95 Ala Ala Lys Lys Tyr Gly Leu Thr Tyr Ile Val Phe Thr Asn Phe Phe 100 105 110 Asp Leu Pro Asp Met Phe Met Glu Arg Ile Pro Glu Asn Ser Pro Phe 115 120 125 Lys Gly Tyr Phe Arg Ala Asn Leu Tyr Arg Ile Thr Ile Val Asn Asp 130 135 140 Pro Ala Asp Phe Ile Arg Asp Glu Gly Ile Ala Glu Val Ala Val Glu 145 150 155 160 Gly Pro Glu Asn Arg Ile Leu Ser Met Val Glu Glu Leu Gly Gln Pro 165 170 175 Met Asn Asp Leu Ser Ile Val Lys Asn Asn Arg Ile Asn Glu His Thr 180 185 190 Phe Tyr Glu Phe Phe Gly Pro Asn Val Gly Lys Asn Gln Ala Leu Asp 195 200 205 Tyr Val Met His His Leu Ala Leu Thr Pro Asp Glu Ile Ala Tyr Ile 210 215 220 Gly Asp Asn Tyr Asn Asp Val Glu Ile Met Lys Val Val Gly Leu Pro 225 230 235 240 Ile Ala Met Ala Asn Ala Pro Lys Glu Val Lys Asp Phe Ala Arg Tyr 245 250 255 Val Thr Thr Arg Thr Asn Asn Glu His Gly Val Ala Glu Ala Val Arg 260 265 270 Lys Ile Ile Arg Glu Glu Ile Arg 275 280 <210> 88 <211> 275 <212> PRT <213> Marinitoga piezophila <400> 88 Met His Asn Lys Thr Phe Ile Phe Asp Leu Asp Gly Thr Leu Leu Asn 1 5 10 15 Ser Asn Glu Ser Ile Ser Gln Arg Thr Val Glu Ser Ile Lys Lys Ile 20 25 30 Phe Glu Met Gly Ser Phe Ile Ile Ile Ala Ser Gly Arg Met Tyr Lys 35 40 45 Ser Thr Arg Leu Ile Val Glu Lys Tyr Met Pro Phe Leu His Asn Asn 50 55 60 Ile Pro Ile Val Ser Tyr Asn Gly Ala Tyr Val Val Ser His Thr Gly 65 70 75 80 Glu Val Val Phe Glu Ser Asp Ile Leu Lys Asp Leu Ala Ile Lys Val 85 90 95 Ile Glu Asp Leu Lys Lys His Asp Thr His Val Gln Ile Tyr Leu Asn 100 105 110 Asp Asp Leu Ile Thr Asp Lys Asp Asn Asp Glu Ile Lys Gln Tyr Ala 115 120 125 Lys His Ser Gly Val Glu Tyr Lys Ile Val Lys Asp Ile Val Glu His 130 135 140 Ile Lys Gln His Gly Glu Pro Thr Lys Ile Leu Ala Ile Asn Gln Pro 145 150 155 160 Glu Lys Leu Asp Ile Ile Gln Lys Ser Met Thr Glu Lys Tyr Gly Asn 165 170 175 Thr Leu Asn Ile Val Arg Ser Phe Ser Ile Tyr Leu Asp Phe Leu Asn 180 185 190 Lys Asp Ser Ser Lys Gly Leu Ala Leu Lys Lys Leu Lys Glu Ile Tyr 195 200 205 Arg Phe Asn Leu Glu Glu Ala Tyr Ile Phe Gly Asp Ser Glu Asn Asp 210 215 220 Ile Ser Met Leu Ser Leu Ser Lys Asn Ser Tyr Ala Met Ala Asn Ala 225 230 235 240 Asn Glu His Val Lys Glu Ser Ala Arg Asn Ile Thr Leu Ser Asn Asp 245 250 255 Glu Glu Gly Val Ala Ile Val Leu Glu Lys Ile Ile Ser Asn Phe Ser 260 265 270 Asn Ser Asn 275 <210> 89 <211> 213 <212> PRT <213> Marinitoga piezophila <400> 89 Met Met Lys Tyr Lys Tyr Leu Phe Phe Asp Met Asp Gly Thr Leu Phe 1 5 10 15 Glu Thr Ser Arg Gly Ile Ile Tyr Ser Leu Gln Lys Ala Tyr Lys Ile 20 25 30 Asn Asn Leu Thr Pro Leu Pro Glu Asp Lys Ile Lys Lys Leu Ile Gly 35 40 45 Pro Thr Leu Asp Glu Ile Met Asn Ile Leu Phe Gly Glu Glu Asn Thr 50 55 60 Glu Leu Lys Met Lys Val Arg Glu Asp Phe Arg Met Thr Tyr Ala Lys 65 70 75 80 Glu Gly Val Phe Met Leu Asp Leu Tyr Pro Glu Val Met Asp Thr Leu 85 90 95 Glu Lys Leu Tyr Ser Lys Asn Arg Ile Met Tyr Ile Val Thr Ser Lys 100 105 110 Pro Glu Ile Phe Ala Ser Gln Ile Leu Glu Lys Phe Asn Met Ser Arg 115 120 125 Phe Phe Lys Lys Ile Cys Gly Val Pro Leu Asp Gly Lys Ile Pro Pro 130 135 140 Lys Ser Glu Arg Leu Lys Asn Leu Ile Ile Glu Glu Asn Ile Pro Leu 145 150 155 160 Lys Glu Gly Leu Met Ile Gly Asp Thr Leu Ser Asp Ala Lys Ala Ala 165 170 175 Trp Glu Asn Asn Val Asp Phe Ala Trp Ile Ser Phe Gly Phe Gly His 180 185 190 Glu Asn Glu Ile Lys Lys Tyr Asp Tyr Lys Ile Asn Asn Phe Ser Gln 195 200 205 Leu Leu Asn Leu Lys 210 <210> 90 <211> 217 <212> PRT <213> Marinitoga piezophila <400> 90 Met Asn Phe Ile Phe Asp Leu Asp Gly Thr Leu Ile Asn Thr Glu Asn 1 5 10 15 Ile Ala Ile Pro Ala Ile Arg Gln Leu Leu Lys Asp Leu Gly Tyr Asn 20 25 30 Pro Asn Ile Glu Lys Glu Glu Ile Leu Gln Tyr Ile Gly Tyr Thr Ile 35 40 45 Asp Asp Ile Phe Tyr Gly Leu Leu Asn Thr Lys Asp Pro Glu Ile Ile 50 55 60 Glu Lys Ala Ile Lys Leu Leu Asp Lys Tyr Glu Ile Glu Ile Ile Glu 65 70 75 80 Lys Leu Pro Lys Asn Glu Ile Phe Phe Asp Gly Ala Ile Glu Val Leu 85 90 95 Lys Thr Leu Lys Asn Glu Asn His Thr Leu Tyr Ile Leu Ser Asn Cys 100 105 110 Asn Lys Lys Tyr Leu Asn Ala Leu Leu Glu Lys Glu Leu Asn Lys Tyr 115 120 125 Ile Asp Phe Pro His Cys Ser Glu Met Tyr Asn Trp Ala Glu Lys Asp 130 135 140 Tyr Val Leu Lys Leu Ile Thr Asn Gly Lys Lys Asp Phe Val Met Ile 145 150 155 160 Gly Asp Arg His Lys Asp Val Asp Ala Ala Lys Lys Asn Gly Ile Leu 165 170 175 Ser Val Gly Cys Thr Tyr Gly Tyr Ala Leu Glu Glu Val Lys Asp Ala 180 185 190 Asp Phe Ile Ile Tyr Asp Ile Lys Glu Leu Leu Thr Ile Lys Asn Lys 195 200 205 Ile Ile Glu Ser Ala Tyr Asn Thr Ile 210 215 <210> 91 <211> 258 <212> PRT <213> Meiothermus cerbereus <400> 91 Met Asp Leu Arg Val Tyr Leu Gln Thr Ala Leu Asp Ala Ala Tyr Leu 1 5 10 15 Ala Lys Gly Ile His Gln Tyr Tyr Gln Glu Lys Gly Phe Thr Leu Ser 20 25 30 Ser Lys Ser Thr Pro Thr Asp Leu Val Thr Gln Ala Asp His Glu Ser 35 40 45 Glu Ala Ala Ile Arg Glu Leu Ile Ala Ala Arg His Pro Asp His Val 50 55 60 Val Leu Gly Glu Glu Gln Gly Gln Asp Arg Glu Gly Ala Phe Arg Trp 65 70 75 80 Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe Pro 85 90 95 Phe Tyr Ala Val Ser Ile Gly Leu Glu Ala His Gly Glu Val Val Leu 100 105 110 Gly Val Ile Leu Asp Thr Ala Arg Gly Asp Leu Phe Thr Ala Thr Lys 115 120 125 Gly Gly Gly Ala Phe Met Asn Gly Arg Pro Ile Arg Val Ser Pro Arg 130 135 140 Ala Thr Leu Val Gly Ser Leu Leu Ala Thr Gly Phe Pro Tyr Asp Val 145 150 155 160 Ser Lys Asp Thr Glu Asn Leu Val Tyr Phe Gln Arg Ala Leu Thr Arg 165 170 175 Gly Leu Met Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala Tyr 180 185 190 Val Ala Ala Gly Arg Leu Asp Gly Phe Trp Glu Val Lys Leu Asn Pro 195 200 205 Trp Asp Val Ala Ala Gly Trp Leu Ile Ile Arg Glu Ala Gly Gly Thr 210 215 220 Val Thr Gly Met Glu Gly Glu Ala Tyr Arg Leu Gly Asn Arg Tyr Leu 225 230 235 240 Val Ala Ser Asn Gly Leu Ile His Gln Pro Leu Leu Asp Thr Ile His 245 250 255 Gly Arg <210> 92 <211> 216 <212> PRT <213> Meiothermus cerbereus <400> 92 Met Lys Leu Lys Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp 1 5 10 15 Thr Asp Arg Leu His Glu Gln Ala Trp Leu Glu Gly Leu Ser Arg Tyr 20 25 30 Gly Leu Gln Gly Asp His Thr Phe Tyr Gln Thr Gln Ile Ser Gly Gly 35 40 45 Leu Asn Pro Glu Ile Val Gln Arg Leu Leu Pro Gln Leu Ser Gln Ala 50 55 60 Glu Ala Glu Ala Phe Leu Glu Gln Lys Glu Ala Arg Phe Arg Glu Leu 65 70 75 80 Ala Ser Glu Val Gln Pro Leu Pro Gly Leu Gly Thr Leu Trp Asp Trp 85 90 95 Ala Gln Glu Gln Asn Leu Arg Arg Ala Leu Val Ser Asn Ala Pro Arg 100 105 110 Glu Asn Ala Gln Tyr Leu Leu Lys Arg Leu Gly Leu Val Phe Asp His 115 120 125 Ile Val Leu Ser Glu Glu Leu Pro Ala Gly Lys Pro Asp Pro Leu Pro 130 135 140 Tyr Arg Thr Ala Leu Gln Ala Leu Asn Ile Gly Pro Ser Glu Ala Leu 145 150 155 160 Ala Phe Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly 165 170 175 Ile Arg Thr Val Ala Leu Thr Thr Gly His Pro Ala His Ala Leu Glu 180 185 190 Gln Ala Gly Ala Phe Leu Cys Ile Pro Asn Phe Ala Asp Pro Arg Leu 195 200 205 Trp Ala Tyr Leu His Lys Met Gly 210 215 <210> 93 <211> 258 <212> PRT <213> Meiothermus chliarophilus <400> 93 Met Asp Leu Arg Pro Phe Leu Glu Thr Ala Leu Ala Ala Ala Tyr Val 1 5 10 15 Ala Arg Gly Ile His Gln Leu Tyr Gln Asp Lys Gly Phe Thr Gln Ser 20 25 30 Thr Lys Ser Thr Pro Thr Asp Val Val Thr Gln Ala Asp Lys Glu Ala 35 40 45 Glu Ala Ala Ile Arg Ala Leu Ile Glu Gln Arg His Pro Gly His Val 50 55 60 Val Leu Gly Glu Glu Glu Gly Gln Gln Gly Glu Gly Glu Tyr Arg Trp 65 70 75 80 Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe Pro 85 90 95 Phe Tyr Cys Val Ser Ile Gly Leu Glu Val Arg Gly Glu Val Met Val 100 105 110 Gly Val Val Leu Asp Thr Ala Arg Gly Asp Leu Phe Thr Ala Thr Arg 115 120 125 Gly Gly Gly Ala Tyr Phe Asn Gly Arg Pro Met Arg Val Ser Arg Ser 130 135 140 Pro Lys Leu Leu Gly Ser Leu Ile Ala Thr Gly Phe Pro Tyr Asp Val 145 150 155 160 Ala Arg Asp Arg Glu Asn Leu Thr Tyr Leu Glu Arg Val Leu Phe Lys 165 170 175 Gly Ile Thr Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala Tyr 180 185 190 Val Ala Cys Gly Arg Leu Asp Gly Phe Trp Glu Val Lys Leu Asn Pro 195 200 205 Trp Asp Val Ala Ala Gly Trp Leu Leu Val Glu Glu Ala Gly Gly Arg 210 215 220 Ile Thr Gly Ile His Gly Glu Pro Tyr Arg Met Gly Asn Arg Tyr Leu 225 230 235 240 Met Ala Ser Asn Gly His Ile His Glu Glu Leu Leu Ala Thr Ile His 245 250 255 Gly Arg <210> 94 <211> 262 <212> PRT <213> Meiothermus chliarophilus <400> 94 Met Asp Leu Asp Trp Thr Leu Val His Pro Ser Gln Gly Val Pro Glu 1 5 10 15 Ser Ala Trp Arg Ala Val Glu Glu Ala Arg Ala Ala Gly Val Arg Leu 20 25 30 Ala Val Ala Thr Gly Arg Pro Phe Gly Gly Phe Gly Leu Glu Tyr Ala 35 40 45 Leu Arg Met Asn Pro Glu Gly Tyr His Ala Phe Ser Asn Gly Ser Leu 50 55 60 Ile Ala Arg Gly Ala Glu Leu Val His Arg Val Ala Met Pro Glu Ala 65 70 75 80 Thr Tyr Arg Arg Met Val Leu His Ser Arg Glu Gln Gly Leu Pro Phe 85 90 95 Tyr Val Ser Gly Ala Ser Gly Arg Leu Tyr Ser Glu Asn Pro Pro Arg 100 105 110 Glu Leu His Arg Phe Ala Leu Arg Met Gly Val Gly Tyr Glu Arg Val 115 120 125 Asp Leu Leu Glu Leu Pro Glu Pro Cys Val Gly Gly Val Phe Val Ile 130 135 140 Thr Gly Gly Leu Trp Asp Ser Leu Arg Pro Ser Leu Thr Ala Val Glu 145 150 155 160 Gly Leu Asp Trp Leu Glu Tyr Val Val Gly Glu Gly Glu Val Val Ala 165 170 175 Val Ala Asp Pro Lys Gly Val Ser Lys Ala Ser Ala Leu Arg Trp Met 180 185 190 Ala Glu Arg Tyr Gly Leu Ala Met Glu Glu Val Ala Met Ile Gly Asp 195 200 205 Ser Leu Asn Asp Leu Glu Ala Ile Arg Gln Val Gly Leu Gly Ile Ala 210 215 220 Met Gly Asn Ala Glu Pro Ala Ile Leu Glu Ala Ala Asp Ala Val Val 225 230 235 240 Gly Arg Leu Glu Glu Asp Gly Phe Ala Gln Ala Val Arg Leu Cys Leu 245 250 255 Glu Pro Ser Glu Val Arg 260 <210> 95 <211> 258 <212> PRT <213> Meiothermus ruber <400> 95 Met Asp Leu Arg Ala Tyr Leu Gln Thr Ala Leu Asp Ala Ala Tyr Leu 1 5 10 15 Ala Lys Gly Ile His Gln Tyr Tyr Gln Glu Lys Gly Phe Thr Gln Ser 20 25 30 Thr Lys Ser Thr Pro Thr Asp Leu Val Thr Gln Ala Asp His Glu Ser 35 40 45 Glu Ala Ala Ile Arg Glu Leu Ile Ala Ser Arg His Pro Asp His Val 50 55 60 Val Leu Gly Glu Glu Gln Gly Gln Asp Lys Glu Gly Ala Phe Arg Trp 65 70 75 80 Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe Pro 85 90 95 Phe Tyr Ala Val Ser Ile Gly Leu Glu Ala His Gly Glu Val Val Leu 100 105 110 Gly Val Val Leu Asp Thr Ala Arg Gly Glu Leu Phe Thr Ala Thr Lys 115 120 125 Gly Gly Gly Ala Tyr Leu Asn Gly Arg Pro Ile Arg Val Ser Thr Arg 130 135 140 Ser Thr Leu Val Gly Ser Leu Leu Ala Thr Gly Phe Pro Tyr Asp Val 145 150 155 160 Ser Lys Asp Thr Glu Asn Leu Val Tyr Phe Gln Arg Ala Leu Thr Lys 165 170 175 Gly Leu Met Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala Tyr 180 185 190 Val Ala Ala Gly Arg Leu Asp Gly Phe Trp Glu Val Lys Leu Asn Pro 195 200 205 Trp Asp Val Ala Ala Gly Trp Leu Ile Val Ser Glu Ala Gly Gly Arg 210 215 220 Ile Thr Gly Leu Gln Gly Glu Asp Tyr Arg Leu Gly Asn Arg Tyr Leu 225 230 235 240 Val Ala Ser Asn Gly Leu Ile His Gly Pro Leu Leu Asp Thr Ile His 245 250 255 Gly Arg <210> 96 <211> 272 <212> PRT <213> Meiothermus ruber <400> 96 Met Ile Arg Leu Val Ala Leu Asp Leu Asp Gly Thr Phe Tyr Ala Gly 1 5 10 15 Arg Ser Leu Gly Val Pro Ala Ser Ala Trp Glu Ala Val Glu Lys Gly 20 25 30 Arg Arg His Gly Leu Arg Phe Ala Val Cys Thr Gly Arg Pro Gln Gly 35 40 45 Gly Tyr Gly Leu Glu Tyr Ala Lys Arg Leu Glu Pro Asn Gly Ala His 50 55 60 Val Phe Asn Asp Gly Ala Ser Val Cys Asp Ala Thr Gly Arg Ser Leu 65 70 75 80 Gln Ala Asp Pro Leu Pro His Leu Ser Glu Leu Val Gly Leu Ala Arg 85 90 95 Ala His Ala Leu Pro Phe Asp Leu Met Gly Ala Glu Gly Gly Arg Tyr 100 105 110 Tyr Glu Glu Gly Leu Met Pro Pro Glu Leu Leu Ser His Ile Glu Thr 115 120 125 Thr Gly Val Glu Ala Arg Ser Ala Arg Leu Glu Glu Ile Glu Glu Thr 130 135 140 Leu Val Arg Leu Trp Phe Val Val Gly Asp Leu Gly Leu Trp Glu Ser 145 150 155 160 Val Lys Pro Glu Leu Val Ala Leu Pro Ser Ile Asp Leu Ala Glu Tyr 165 170 175 Ile Ser Pro Arg Glu Val Ile Ala Gly Val Ile Arg Lys Gly Val Ser 180 185 190 Lys Ala Thr Gly Leu Arg Trp Leu Ala Gln Tyr Tyr Gly Leu Ser Leu 195 200 205 Ser Glu Ile Ala Met Ile Gly Asp Ser His Asn Asp Leu Glu Ala Ile 210 215 220 Arg Glu Ala Gly Leu Gly Ile Ala Met Gly Asn Ala Val Glu Ala Ile 225 230 235 240 Arg Ala Ala Ala Arg His Ile Thr Gly His Val Arg Glu Asp Gly Phe 245 250 255 Ala Glu Ala Val Glu Tyr Ile Leu Ala Tyr Asn Arg Gln Asn Gln Leu 260 265 270 <210> 97 <211> 278 <212> PRT <213> Meiothermus ruber <400> 97 Met Leu Gln Lys Ile Ser Arg Leu Pro Ile Gln Leu Val Leu Ile Asp 1 5 10 15 Ile Asp Gly Thr Leu Phe Gly Pro Gln Gly Val Pro Glu Cys Ala Trp 20 25 30 Glu Ala Ala Gln Arg Ala Arg Ala Ala Gly Leu His Leu Ser Ile Cys 35 40 45 Thr Gly Arg Pro Gly Arg Gly Phe Ala Leu Glu Tyr Ala Lys Arg Leu 50 55 60 Asp Pro Ala Gly Leu His Ile Phe Glu Ser Gly Ala Val Val Val Ser 65 70 75 80 Gly Gln Gly Glu Val Val Gln Ala Ser Thr Leu Pro Pro Ser Val Tyr 85 90 95 Gln Arg Leu Leu Ala Leu Ser Arg Ala Tyr Gly Leu Pro Phe Glu Val 100 105 110 Tyr Thr Ala Glu Gly Gly Phe Tyr Arg Glu Ser Gln His Pro Asp Leu 115 120 125 Val Phe His Glu Ser Met Leu Gly Cys Pro Ala Val Val Cys Gly Leu 130 135 140 Asp Asp Val Gly Ala Gln Val Val Arg Val Gln Phe Val Trp Arg Ala 145 150 155 160 Ser Pro Ala Trp Gln Ala Val Arg Ala Gln Ile Ala Gln Met Thr Glu 165 170 175 Val Glu Leu His Glu Ala Thr Ser Pro Gly Met Pro Gly Val Gly Phe 180 185 190 Ser Ser Val Thr Ala Ala Gly Val Ser Lys Arg Ala Ala Ala Glu Trp 195 200 205 Val Ala Ala Arg Leu Asp Leu Asp Leu Ser Arg Cys Ala Met Val Gly 210 215 220 Asp Gly Glu Asn Asp Leu Glu Leu Ile Gln Ala Ala Gly Leu Gly Ile 225 230 235 240 Ala Met Gly Asn Ala Pro Glu Arg Val Lys Gln Ala Ala Gln Arg Val 245 250 255 Val Gly Pro Val Glu Ala Cys Gly Leu Glu Gln Ala Leu Glu Ile Ile 260 265 270 Gln Asn His Ala Arg Ser 275 <210> 98 <211> 216 <212> PRT <213> Meiothermus ruber <400> 98 Met Lys Leu Arg Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp 1 5 10 15 Thr Asp Arg Leu His Glu Gln Ala Trp Leu Glu Gly Leu Ser Arg Tyr 20 25 30 Gly Ile Gln Gly Asp His His Phe Tyr Gln Thr Gln Ile Ser Gly Gly 35 40 45 Leu Asn Pro Glu Ile Val Gln Arg Leu Leu Pro Gln Leu Ser Glu Ala 50 55 60 Glu Gly Gln Arg Phe Ile Glu Gln Lys Glu Ala Arg Phe Arg Glu Leu 65 70 75 80 Ala Thr Thr Val Gln Pro Leu Pro Gly Leu Arg Val Leu Trp Asn Trp 85 90 95 Ala Gly Glu Arg Gly Leu Gly Arg Ala Leu Val Ser Asn Ala Pro Arg 100 105 110 Gly Asn Ala Leu Tyr Leu Leu Glu Arg Leu Gly Leu Val Phe Asp His 115 120 125 Ile Val Leu Ser Glu Asp Leu Pro Ala Gly Lys Pro Asp Pro Leu Pro 130 135 140 Tyr Arg Met Ala Leu Gln His Leu Asn Leu Ala Pro Gln Glu Ala Leu 145 150 155 160 Ala Phe Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly 165 170 175 Leu Pro Thr Val Ala Leu Thr Thr Gly His Pro Pro Glu Ala Leu Ala 180 185 190 Gln Ala Gly Ala Phe Leu Cys Ile Pro Asp Tyr Thr Asp Pro Arg Leu 195 200 205 Trp Asp Trp Leu Arg Ala Leu Gly 210 215 <210> 99 <211> 258 <212> PRT <213> Meiothermus ruber <400> 99 Met Asp Leu Arg Pro Tyr Leu Glu Ala Ala Leu Asp Ala Ala Tyr Leu 1 5 10 15 Ala Lys Gly Ile His Gln Tyr Tyr Gln Glu Lys Gly Phe Thr His Ser 20 25 30 Thr Lys Ser Thr Pro Thr Asp Val Val Thr Gln Ala Asp His Glu Ala 35 40 45 Glu Ala Ala Ile Arg Ala Leu Ile Ala Glu Arg Phe Pro Asp His Val 50 55 60 Val Leu Gly Glu Glu Gln Gly Gln Glu Gly Glu Gly Glu Phe Arg Trp 65 70 75 80 Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe Pro 85 90 95 Phe Tyr Gly Val Ser Ile Gly Leu Glu Val Arg Gly Glu Val Val Leu 100 105 110 Gly Val Val Leu Asp Thr Ala Arg Gly Asp Leu Phe Thr Ala Thr Lys 115 120 125 Gly Gly Gly Ala Tyr Leu Asn Gly Arg Pro Leu Arg Val Ser Gln Arg 130 135 140 Thr Thr Leu Val Gly Ser Leu Leu Ala Thr Gly Phe Pro Tyr Asp Val 145 150 155 160 Ala Arg Asp Pro Glu Asn Leu Thr Tyr Phe Gln Arg Ala Leu Ala Lys 165 170 175 Gly Leu Thr Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala Asn 180 185 190 Val Ala Ala Gly Arg Leu Asp Gly Phe Trp Glu Val Lys Leu Asn Pro 195 200 205 Trp Asp Val Ala Ala Gly Trp Leu Leu Ile Thr Glu Ala Gly Gly Gln 210 215 220 Val Thr Gly Phe Gln Gly Glu Ala Tyr Arg Leu Gly Asn Arg Tyr Leu 225 230 235 240 Val Ala Ser Asn Gly Arg Ile His Gln Ala Leu Leu Glu Ala Leu Gln 245 250 255 Gly Arg <210> 100 <211> 258 <212> PRT <213> Meiothermus silvanus <400> 100 Met Asp Leu Gln Arg Tyr Leu Glu Ala Ala Leu Asp Ala Ala Tyr Leu 1 5 10 15 Ala Arg Gly Ile His Leu Tyr Tyr Arg Glu Lys Gly Phe Ala Leu Glu 20 25 30 Ser Lys Thr Ser Pro Thr Asp Leu Val Thr Gln Ala Asp Arg Glu Ser 35 40 45 Glu Glu Ala Ile Arg Ser Leu Leu Leu Glu Arg Phe Pro Asp His Val 50 55 60 Val Leu Gly Glu Glu Gly Gly Gln Glu Gly His Ser Asp Phe Arg Trp 65 70 75 80 Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe Pro 85 90 95 Phe Tyr Ala Val Ser Ile Ala Leu Glu Val Gly Gly Glu Val Val Val 100 105 110 Gly Ala Val Leu Asp Thr Ala Arg Gly Glu Leu Phe Thr Ala Leu Lys 115 120 125 Gly Glu Gly Ala Phe Gln Asn Gly Arg Pro Ile Arg Val Ser Ser Thr 130 135 140 Ala Thr Leu Ile Gly Ser Leu Leu Ala Thr Gly Phe Pro Tyr Asp Val 145 150 155 160 Ala Lys Asp Ala Glu Asn Leu Thr Tyr Phe Gln Arg Ala Leu Ala Lys 165 170 175 Gly Leu Thr Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala Tyr 180 185 190 Val Ala Ala Gly Arg Leu Glu Gly Phe Trp Glu Val Lys Leu Asn Pro 195 200 205 Trp Asp Val Ala Ala Gly Trp Leu Leu Ile Thr Glu Ala Gly Gly Thr 210 215 220 Val Ser Gly Ile Gln Gly Glu Pro Tyr Arg Leu Gly Asn Arg Tyr Leu 225 230 235 240 Val Ala Ser Asn Gly Lys Ile His Glu Gln Leu Leu Asp Thr Leu His 245 250 255 Gly Arg <210> 101 <211> 213 <212> PRT <213> Meiothermus silvanus <400> 101 Met Arg Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asn Thr Asp 1 5 10 15 Arg Leu His Glu Gln Ala Trp Leu Glu Thr Leu Arg Phe Tyr Gly Ile 20 25 30 Glu Gly Asp His His Phe Tyr Gln Thr Gln Ile Ser Gly Gly Leu Asn 35 40 45 Pro Glu Ile Val Arg Arg Leu Leu Pro Gln Leu Ser Glu Ala Glu Gly 50 55 60 Glu Ala Phe Ile Ala Arg Lys Glu Arg Arg Phe Arg Glu Leu Ala Gln 65 70 75 80 Asp Leu Arg Ala Leu Pro Gly Leu Asp Ala Leu Leu Ala Trp Ala Arg 85 90 95 Arg Lys Lys Leu Leu Thr Gly Leu Val Thr Asn Ala Pro His Glu Asn 100 105 110 Ala Arg His Val Thr Gln Ala Leu Gly Leu Ser Phe Asp Val Val Val 115 120 125 Leu Ala Glu Glu Leu Ala Ala Gly Lys Pro Asp Pro Leu Pro Tyr Arg 130 135 140 Val Ala Leu Glu Arg Leu Asp Leu Gly Ala Gln Glu Ala Leu Ala Phe 145 150 155 160 Glu Asp Ser Pro Ala Gly Val Lys Ala Ala Val Gly Ala Gly Ile Pro 165 170 175 Thr Ile Gly Leu Thr Thr Gly His Pro Pro Glu Ala Leu Lys Ala Ala 180 185 190 Gly Ala Phe Leu Leu Ile Ala Asp Phe Thr Asp Pro Gln Leu Trp Lys 195 200 205 Tyr Leu Glu Arg Ser 210 <210> 102 <211> 258 <212> PRT <213> Meiothermus taiwanensis <400> 102 Met Asp Leu Arg Ala Tyr Leu Gln Thr Ala Leu Asp Ala Ala Tyr Leu 1 5 10 15 Ala Lys Gly Ile His Gln Tyr Tyr Gln Glu Lys Gly Phe Thr Gln Ser 20 25 30 Ser Lys Ser Thr Pro Thr Asp Leu Val Thr Gln Ala Asp His Glu Ser 35 40 45 Glu Ala Ala Ile Arg Glu Leu Ile Ala Ala Arg His Pro Asp His Val 50 55 60 Val Leu Gly Glu Glu Gln Gly Gln Asp Lys Glu Gly Ala Phe Arg Trp 65 70 75 80 Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe Pro 85 90 95 Phe Tyr Ala Val Ser Ile Gly Leu Glu Ala His Gly Glu Val Val Leu 100 105 110 Gly Val Val Leu Asp Thr Ala Arg Gly Glu Leu Phe Thr Ala Thr Lys 115 120 125 Gly Gly Gly Ala Tyr Leu Asn Gly Arg Pro Ile Arg Val Ser Thr Arg 130 135 140 Ser Thr Leu Val Gly Ser Leu Leu Ala Thr Gly Phe Pro Tyr Asp Val 145 150 155 160 Ser Lys Asp Thr Glu Asn Leu Thr Tyr Phe Gln Arg Ala Leu Thr Lys 165 170 175 Gly Leu Met Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala Tyr 180 185 190 Val Ala Ala Gly Arg Leu Asp Gly Phe Trp Glu Val Lys Leu Asn Pro 195 200 205 Trp Asp Val Ala Ala Gly Trp Leu Ile Val Ser Glu Ala Gly Gly Arg 210 215 220 Val Ser Gly Leu Gln Gly Glu Asp Tyr Arg Leu Gly Asn Arg Tyr Leu 225 230 235 240 Val Ala Ser Asn Gly Leu Ile His Gln Pro Leu Leu Asp Thr Leu His 245 250 255 Gly Arg <210> 103 <211> 216 <212> PRT <213> Meiothermus taiwanensis <400> 103 Met Lys Leu Arg Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp 1 5 10 15 Thr Asp Arg Leu His Glu Gln Ala Trp Leu Glu Gly Leu Ser Arg Tyr 20 25 30 Gly Ile Gln Gly Asp His Arg Phe Tyr Gln Ala Gln Ile Ser Gly Gly 35 40 45 Leu Asn Pro Glu Ile Val Ala Arg Leu Leu Pro Gln Leu Ser Pro Asp 50 55 60 Glu Gln Val Ala Phe Ile Glu Gln Lys Glu Ala Arg Phe Arg Glu Leu 65 70 75 80 Ala Ser Glu Val Gln Pro Leu Pro Gly Leu Arg Val Leu Trp Asp Trp 85 90 95 Ala Gln Ser Gln Gly Leu Arg Arg Ala Leu Val Ser Asn Ala Pro Arg 100 105 110 Glu Asn Ala His Tyr Leu Leu Glu Arg Leu Gly Leu Met Phe Asp Ala 115 120 125 Ile Val Leu Ser Glu Asp Leu Pro Ala Gly Lys Pro Asp Pro Leu Pro 130 135 140 Tyr Arg Thr Ala Leu Gln His Leu Asn Ile Gly Pro Gln Glu Ala Leu 145 150 155 160 Ala Phe Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly 165 170 175 Leu Arg Thr Val Ala Leu Thr Thr Gly His Pro Pro His Ala Leu Glu 180 185 190 Gln Ala Gly Ala Phe Leu Cys Ile Pro Asp Phe Thr Asp Pro Arg Leu 195 200 205 Trp Asp Trp Leu Gln Lys Met Gly 210 215 <210> 104 <211> 271 <212> PRT <213> Meiothermus timidus <400> 104 Met Ile Gly Leu Val Leu Ile Asp Val Asp Gly Thr Leu Tyr Gly Pro 1 5 10 15 Asp Gly Val Pro Glu Cys Ala Trp Glu Ala Thr Gln Lys Ala Arg Ala 20 25 30 Ala Gly Ile His Leu Ala Leu Cys Thr Gly Arg Pro Gly Arg Gly Phe 35 40 45 Ala Leu Glu Tyr Ala Arg Arg Ile Asp Pro Glu Gly Leu His Ile Phe 50 55 60 Glu Ser Gly Ala Val Val Val Ala Gly Asn Gly Gln Val Ile Lys Ser 65 70 75 80 Ser Pro Leu Ser Pro Glu Ala Tyr Arg Glu Leu Leu Ala Leu Ser Arg 85 90 95 Glu Tyr Ala Ile Pro Phe Glu Val Tyr Thr Ala Asp Gly Gly Phe Phe 100 105 110 Ile Glu Gln Asn His Pro Asp Leu Glu Ala His Gln Arg Met Leu Gly 115 120 125 Phe Met Ala Lys Val Ala Pro Leu Asp Gln Pro Pro Gly Glu Val Ile 130 135 140 Arg Val Gln Tyr Val Thr Pro Leu Glu Gly Gln Trp Gln Glu Val Arg 145 150 155 160 Gly Arg Val Gln Arg Ile Glu Gly Ala Glu Leu His Glu Ala Thr Ser 165 170 175 Pro Gly Val Pro Gly Ile Gly Phe Asn Ser Val Thr Ala Ala Gly Val 180 185 190 Ser Lys Leu Ser Ala Ala Arg Trp Val Ala Glu Arg Tyr Gly Leu Gly 195 200 205 Leu Glu His Cys Ala Met Val Gly Asp Gly Glu Asn Asp Leu Glu Leu 210 215 220 Ile Leu Ala Ala Gly Val Gly Ile Ala Met Gly Asn Ala Pro Gln Ser 225 230 235 240 Val Lys Glu Arg Ser Lys Arg Val Val Gly Arg Val Glu Glu Cys Gly 245 250 255 Leu Ala Gln Ala Leu Glu Trp Val Ala Ala Ala Glu Gly Arg Ser 260 265 270 <210> 105 <211> 214 <212> PRT <213> Meiothermus timidus <400> 105 Met Arg Pro Thr Thr Leu Leu Phe Asp Leu Asp Gly Thr Leu Leu Asp 1 5 10 15 Thr Glu Pro Gly Ile Leu Ala Cys Leu Arg His Thr Leu Glu His Phe 20 25 30 Gly Leu Pro Thr Gln Gly Asp Ala Glu Leu Arg Arg Phe Ile Gly Pro 35 40 45 Pro Leu Glu Gln Ala Trp Arg Glu Leu Val Gly Ala Ala His Val Glu 50 55 60 Glu Ala Val Glu Ile Tyr Arg Arg Cys Tyr Asp Leu Lys Gly Lys Phe 65 70 75 80 Lys Ala Ala Val Phe Glu Gly Ile Glu Ala Ala Leu His Glu Leu Ala 85 90 95 Tyr His His Thr Leu Ile Val Ala Thr Ser Lys Arg Gln Val Phe Ala 100 105 110 Glu Glu Met Leu Glu Arg Phe Gly Leu Ser Ser Tyr Phe Lys Ala Val 115 120 125 Tyr Gly Val Thr Pro Pro Gln Leu Ser Glu Pro Lys Ala His Leu Ile 130 135 140 Gly Arg Ile Leu Arg Asp His Gly Leu Ser Pro Gln Gln Ala Val Met 145 150 155 160 Val Gly Asp Arg Glu Phe Asp Val Ile Gly Ala Lys Ala Asn Gly Met 165 170 175 Ala Cys Val Gly Val Leu Trp Gly Tyr Gly Ser Arg Glu Glu Leu Ala 180 185 190 Phe His Gly Ala Ser Leu Leu Cys Glu Ser Pro Arg Glu Leu Val Glu 195 200 205 Ala Val Ala Gly Leu Ala 210 <210> 106 <211> 223 <212> PRT <213> Meiothermus timidus <400> 106 Met Lys Arg Ala Val Leu Phe Asp Val Gly Asp Thr Leu Ile Leu Gly 1 5 10 15 His Pro Lys Leu Trp Leu Trp Pro Leu Leu Gln Glu Arg Gly Leu Ala 20 25 30 Glu Lys Ala Asp Gly Ser Arg Leu Met Glu Ala Val Met Ala Ala Tyr 35 40 45 Arg Val Tyr Asn Glu Arg His Met Gln Ala Thr Thr Ile Glu Ala Ala 50 55 60 Leu Pro Val Trp Ala Glu Phe His Arg Thr Leu Leu Ser Gly Ile Gly 65 70 75 80 Leu Gly Glu His Ala Gly Glu Ile Ser Gly Phe Leu Cys Glu Asn Trp 85 90 95 Arg Asn Pro Lys Val Trp Pro Leu Thr Pro Gly Ala Lys Glu Val Leu 100 105 110 Gly Glu Leu Lys Gly Arg Gly Tyr Lys Leu Gly Val Val Ser Asn Trp 115 120 125 Asp Ala Leu Leu Pro Gly Val Leu Glu Ala Thr Gly Leu Ala Pro Phe 130 135 140 Phe Asp Phe Val Ala Ala Ser Ala Leu Val Gly Ala Ala Lys Pro Asp 145 150 155 160 Pro Arg Ile Phe Arg Val Ala Leu Glu Gly Leu Gly Val Glu Pro His 165 170 175 Gln Ala Leu His Val Gly Asp Ser Pro Asp Asp Leu Gly Gly Ala Ala 180 185 190 Ala Ala Gly Val Glu Ala Leu Leu Phe Asp Pro Tyr Arg Arg Asn Pro 195 200 205 Glu Ala Leu His Glu Leu Ser Gly Val Leu Glu Arg Leu Glu Ala 210 215 220 <210> 107 <211> 198 <212> PRT <213> Meiothermus timidus <400> 107 Met Ser Lys Arg Ile Arg Ala Ile Cys Phe Asp Phe Asp Gly Thr Leu 1 5 10 15 Ala Gln Phe Glu Gly Asp Phe Ala Arg Leu Thr Ala Leu Cys Cys Asp 20 25 30 Gln Leu Gly Ile Asp Pro Gly Asp Arg Ala Phe Leu Ala Thr Tyr Glu 35 40 45 Arg Glu Ile Arg Ala Glu Gly His Val Thr Phe Ala Leu Ala Leu Arg 50 55 60 Arg Ala Leu Glu Thr His Gly Tyr Pro Val Pro Ala Gly Leu Glu Ala 65 70 75 80 Phe Ala Ala Glu Val Thr Ala Arg Tyr Ala Ala Glu Val Arg Leu Leu 85 90 95 Pro Gly Val Arg Glu Leu Leu Ala Tyr Phe Ala Pro Leu Pro Lys Ala 100 105 110 Ile Ile Thr Asn Gly Pro Ala Asp Met Gln Trp Ala Ala Ile Arg Ser 115 120 125 Val Gly Leu Glu Arg Glu Phe Glu Ala Ile Val Val Ser Gly Asp Pro 130 135 140 Asp Val Ala Val Arg Lys Pro His Pro Arg Ile Phe His Leu Ala Cys 145 150 155 160 Glu Arg Leu Gly Val Pro Pro His Glu Thr Leu Met Ile Gly Asp His 165 170 175 Leu Glu Ala Asp Ile Gln Gly Ala Ile Ala Ala Gly Leu Gln Gly Leu 180 185 190 His Arg Pro Asn Ser Ala 195 <210> 108 <211> 237 <212> PRT <213> Meiothermus timidus <400> 108 Met Ile Pro Glu Glu Leu Ala Leu Leu Ala Arg Gln Pro Leu Leu Val 1 5 10 15 Ile Cys Asp Tyr Asp Gly Thr Leu Ala Pro Ile Ala Ile His Pro Ser 20 25 30 Gln Ala Phe Pro Gln Arg Gly Ala Arg Glu Ala Leu Arg Ala Leu Gln 35 40 45 His His Pro Lys His Gln Ile Met Val Leu Thr Gly Arg Arg Ala Gln 50 55 60 Glu Val Leu Asp Phe Leu Glu Leu Pro Gly Leu Arg Val Ile Gly Leu 65 70 75 80 His Gly Leu Glu Trp Pro Gly Arg Pro Glu Pro Pro Arg Asp Arg Glu 85 90 95 Ala Ile Gln Arg Ile Val Ser Arg Leu Pro Pro Leu Glu Gly Leu Trp 100 105 110 Leu Glu Asp Lys Gly Trp Thr Leu Ala Val His Tyr Arg Asp Ala Pro 115 120 125 Glu Ser Gln Gln Ser Lys Ala Ala Glu Leu Leu Ala Ala Val Pro Leu 130 135 140 Pro Glu Gly Trp Glu Ala Met Pro Gly Lys Lys Val Cys Glu Tyr Arg 145 150 155 160 Pro Ile Gly Tyr Gly Lys Gly Trp Ala Val Glu Gln Leu Met Leu Asp 165 170 175 His Pro Ser His His Pro Val Phe Ile Gly Asp Asp Arg Thr Asp Glu 180 185 190 Glu Gly Phe Ala Ala Val Leu Arg Leu Gly Gly Thr Ala Val Lys Val 195 200 205 Gly Gly Gly Glu Ser Leu Ala Pro Tyr Arg Leu Gly Asn Ser Leu Glu 210 215 220 Val Val Glu Leu Leu Thr Arg Trp Ala Arg Gly Tyr Asp 225 230 235 <210> 109 <211> 249 <212> PRT <213> Meiothermus timidus <400> 109 Met Arg Ala Leu Ile Phe Asp Val Asp Gly Val Ile Ala Glu Thr Glu 1 5 10 15 Glu Gly His Arg Leu Ala Phe Asn Arg Ala Phe Ala Glu Ala Gly Leu 20 25 30 Asp Ile Glu Trp Ser Gln Glu Leu Tyr Glu Arg Leu Leu Trp Val Thr 35 40 45 Gly Gly Lys Glu Arg Ile Ala His Tyr Leu Tyr His Cys Pro Glu Cys 50 55 60 Pro Lys Leu Leu Asp Ala Asp Ile Ala Arg Leu His Trp Arg Lys Thr 65 70 75 80 Glu Leu Tyr Asn Gln Ile Val Ala Ala Gly Gly Val Pro Phe Arg Pro 85 90 95 Gly Val Leu Arg Leu Trp Arg Glu Ala Arg Ala Gln Gly Val Lys Leu 100 105 110 Ala Ile Ala Thr Thr Thr Ser Leu Pro Asn Val Glu Val Leu Leu Arg 115 120 125 Gln Ala Gly Glu Glu Val Leu Gly Trp Phe Glu Thr Ile Val Ala Gly 130 135 140 Asp Met Val Pro Arg Lys Lys Pro Ala Pro Asp Val Tyr Leu Lys Val 145 150 155 160 Leu Glu Asn Leu Gly Leu Ala Pro Glu Glu Ala Leu Ala Ile Glu Asp 165 170 175 Ser Gln Asn Gly Leu Ile Ala Ala Gln Lys Ala Gly Ile Pro Thr Leu 180 185 190 Ile Thr Tyr Ser His Tyr Thr Arg Glu Gln Arg Phe Glu Gly Ala Leu 195 200 205 Ala Val Leu Glu His Leu Gly Glu Pro Glu Leu Pro Ala Arg Val Val 210 215 220 Ala Gly Pro Arg Ser Cys Ala Met Val Val Ser Leu Glu Val Leu Arg 225 230 235 240 Glu Trp Tyr Gly Arg Phe Val Arg Ala 245 <210> 110 <211> 157 <212> PRT <213> Meiothermus timidus <400> 110 Met Leu Arg Pro Lys Ala Gln Leu Ser Ser Val Leu Glu Ile Thr Pro 1 5 10 15 Gln Trp Leu Lys Thr Arg Gly Ile Lys Gly Leu Leu Leu Asp Leu Asp 20 25 30 Asn Thr Leu Val Pro Tyr Lys Phe Lys Gly Glu Pro Pro Glu Glu Leu 35 40 45 Val Ala Trp Val Lys Ser Leu Glu Ala Ala Gly Ile Arg Val Phe Leu 50 55 60 Val Ser Asn Ala Gln His Lys Arg Leu Arg Ala Trp Ser Ala Lys Leu 65 70 75 80 Gly Val Glu Gly Ile Gly Leu Ala Gly Lys Pro Trp Phe Gly Ile Arg 85 90 95 Lys Gly Leu Lys Arg Leu Gly Leu Ser Pro Ser Gln Val Ala Met Val 100 105 110 Gly Asp Gln Val Phe Thr Asp Val Leu Gly Gly Asn Leu Ala Gly Val 115 120 125 Tyr Thr Ile Leu Val Thr Pro Ile Ser Gln Lys Glu Leu Gly Tyr Thr 130 135 140 Lys Leu Ile Arg Arg Leu Glu Arg Leu Ile Leu Lys Leu 145 150 155 <210> 111 <211> 221 <212> PRT <213> Meiothermus timidus <400> 111 Met Lys Ala Leu Val Phe Asp Phe Asp Gly Thr Ile Leu Asp Thr Glu 1 5 10 15 Gln Ala Glu Tyr Thr Ala Trp Thr Gln Ala Tyr Ala Arg Tyr Gly Gly 20 25 30 Glu Leu Thr Leu His Asp Tyr Leu Pro Leu Ile Gly Thr Ala Glu Pro 35 40 45 Val Phe Asp Leu Tyr Ala His Leu Glu Ala Gln Ile Gly Pro Tyr Asp 50 55 60 Arg Glu Ala Phe Asp Arg Glu Arg Gln Glu Val Leu His Ser Leu Leu 65 70 75 80 Ala Gln Leu Glu Pro Leu Pro Gly Val Pro Glu Cys Leu Glu Gln Ala 85 90 95 Arg Lys Leu Gly Leu Lys Leu Ala Val Ala Ser Ser Ser Ser Ala Ala 100 105 110 Trp Val Leu Gly His Leu Glu Gln His His Leu Leu Gln Ala Phe Asp 115 120 125 His Val Ile Thr Arg Glu Tyr Val Ala Arg Thr Lys Pro Asp Pro Ala 130 135 140 Leu Phe Leu Lys Ala Ala Glu Ala Leu Gln Val Ala Pro Ala Glu Cys 145 150 155 160 Val Ala Val Glu Asp Ser Leu Asn Gly Val Arg Ala Ala Arg Ala Ala 165 170 175 Gly Met Phe Thr Ile Ala Val Pro Asn Pro Leu Thr Arg His Gln Asp 180 185 190 Leu Arg Gln Ala Asp Val Leu Leu Pro Ser Leu Ala Gly Leu Glu Leu 195 200 205 Glu Pro Phe Leu Ser Ser Leu Lys Pro Val His Pro Arg 210 215 220 <210> 112 <211> 274 <212> PRT <213> Meiothermus timidus <400> 112 Met Gly Gly Arg Cys Arg Gly Pro Val Val Ile Arg Leu Ile Gly Val 1 5 10 15 Asp Leu Asp Trp Thr Phe Val His Pro Ser Gln Gly Val Pro Ala Ser 20 25 30 Ala Trp Gln Ala Val Glu Glu Ala Lys Ala Ala Gly Met Arg Phe Ala 35 40 45 Val Val Thr Gly Arg Pro Phe Gly Gly Tyr Gly Leu Glu Tyr Ala Leu 50 55 60 Lys Met Glu Pro Gln Gly Tyr His Ala Phe Ser Asn Gly Ser Leu Ile 65 70 75 80 Thr Gln Gly Ala Thr Leu Val His Ser Val Ala Met Ser Pro Ser Thr 85 90 95 Tyr Arg Arg Met Val Leu His Ser Arg Ala Gln Gly Leu Pro Phe Tyr 100 105 110 Val Ser Gly Ala Ser Gly Arg Leu Tyr Ser Glu Asn Pro Pro Arg Glu 115 120 125 Leu His Arg Phe Ala Glu Arg Met Gly Val Ser Tyr Gln Arg Ile Asp 130 135 140 Leu Leu Glu Leu Pro Glu Pro Cys Val Gly Gly Val Phe Val Leu Asp 145 150 155 160 Gly Gln Leu Trp Asn Asp Leu Arg Gln Ala Ile Thr Ala Val Glu Gly 165 170 175 Leu Asp Trp Leu Glu Tyr Leu Val Gly Glu Gly Glu Val Val Ala Val 180 185 190 Ala Asp Pro Lys Gly Thr Ser Lys Ala Ser Ala Leu Arg Trp Met Ala 195 200 205 Glu Arg Tyr Gly Leu Ala Met Glu Glu Val Ala Met Ile Gly Asp Ser 210 215 220 Leu Asn Asp Leu Glu Ala Ile Lys Gln Val Gly Leu Gly Ile Ala Met 225 230 235 240 Gly Asn Ala Glu Glu Val Val Arg Glu Ala Ala Ser Val Val Val Ala 245 250 255 Gly Leu Glu Glu Asp Gly Phe Ala Gln Ala Val Arg Leu Cys Leu Ala 260 265 270 Arg Arg <210> 113 <211> 216 <212> PRT <213> Meiothermus timidus <400> 113 Met Arg Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp Thr Asp 1 5 10 15 Arg Leu His Glu Gln Ala Trp Leu Glu Val Leu Leu Pro Tyr Gly Ile 20 25 30 Arg Gly Asp His Ala Phe Tyr Gln Gln His Ile Ser Gly His Leu Asn 35 40 45 Pro Glu Ile Val Ser Arg Leu Leu Pro His Leu Pro Pro Leu Glu Arg 50 55 60 Thr Ala Leu Ile Glu Val Lys Glu Arg Arg Phe Arg Glu Leu Ala Gln 65 70 75 80 Gly Leu Lys Ala Leu Pro Gly Leu Glu Gly Leu Trp Arg Trp Ala Arg 85 90 95 Glu Arg Gly Leu Thr Leu Ala Leu Val Thr Asn Ala Pro Arg Pro Asn 100 105 110 Ala Glu His Val Leu Gln Ala Leu Gly Ala Glu Phe Asp Leu Val Val 115 120 125 Leu Ala Glu Glu Leu Ala Ala Gly Lys Pro Asp Pro Leu Pro Tyr Arg 130 135 140 Thr Ala Leu Gly Arg Leu Gly Leu Asp Pro Ala Glu Ala Leu Ala Phe 145 150 155 160 Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly Ile Arg 165 170 175 Thr Ile Gly Leu Thr Thr Gly His Asp Pro Arg Gly Leu Leu Glu Ala 180 185 190 Gly Ala Phe Leu Leu Ile Asp Asp Phe Ser Asp Gly Arg Leu Trp Glu 195 200 205 Tyr Leu Glu Gly Glu Ser Asp Ser 210 215 <210> 114 <211> 223 <212> PRT <213> Meiothermus rufus <400> 114 Met Lys Leu Lys Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp 1 5 10 15 Thr Asp Arg Leu His Glu Gln Ala Trp Leu Glu Gly Leu Ala Arg Tyr 20 25 30 Gly Ile Gln Ala Asp His His Tyr Tyr Gln Thr Gln Ile Ser Gly Gly 35 40 45 Leu Asn Pro Glu Ile Val Ala Arg Leu Leu Pro Gln Leu Ser Pro Ala 50 55 60 Glu Ala Ala Ala Phe Ile Glu Gln Lys Glu Ala Arg Phe Arg Glu Leu 65 70 75 80 Ala Ala Gly Val Thr Pro Leu Pro Gly Leu Gln Glu Leu Trp Ala Trp 85 90 95 Ala Asn Gly His Arg Leu Arg Leu Ala Leu Val Ser Asn Ala Pro Arg 100 105 110 Gln Asn Ala Glu His Met Leu Lys Arg Leu Gly Leu His Phe Asp Cys 115 120 125 Thr Val Leu Ser Glu Glu Leu Pro Ala Gly Lys Pro Asp Pro Leu Pro 130 135 140 Tyr Arg Thr Ala Leu Ala Gln Leu Gly Leu Gly Pro Glu Glu Ala Leu 145 150 155 160 Ala Phe Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly 165 170 175 Leu Arg Thr Val Ala Leu Thr Thr Gly His Pro Ala His Arg Leu Glu 180 185 190 Glu Ala Gly Ala Phe Leu Ala Val Ala Asp Phe Thr Asp Glu Arg Leu 195 200 205 Trp Ala Trp Leu Glu Gln Gln Ser Pro Pro Ser Thr Pro Arg Glu 210 215 220 <210> 115 <211> 218 <212> PRT <213> Mesotoga infera <400> 115 Met Ile Lys Ala Ala Ile Phe Asp Met Asp Gly Val Ile Ile Asp Ser 1 5 10 15 Glu Lys Ile Tyr Arg Arg Ala Cys Thr Glu Leu Val Asp Glu Leu Gly 20 25 30 Gly Lys Ile Ser Val Glu Leu Phe Glu Arg Gln Met Gly Leu Lys Met 35 40 45 Ser Glu Thr Gln Lys Val Val Val Gln Thr Ala Gly Leu Glu Ile Glu 50 55 60 Pro Glu Glu Phe Gly Lys Arg Tyr Met Glu Arg Tyr Leu Glu Leu Ala 65 70 75 80 Arg Glu Thr Leu Val Pro Asn Pro Gly Leu Val Asn Leu Leu Asp Phe 85 90 95 Leu Ser Glu Lys Val Glu Leu Ala Ile Ala Ser Ser Thr Glu Lys Ala 100 105 110 Ala Val Glu Glu Leu Met Lys Arg Ile Gly Val Leu Asp Tyr Phe Glu 115 120 125 Ile Ile Val Gly Gly Asp Glu Val Asp Glu Ser Lys Pro Ser Pro Met 130 135 140 Ile Tyr Leu Lys Ala Ser Glu Leu Leu Gly Val Asn Pro Glu Glu Cys 145 150 155 160 Ile Val Ile Glu Asp Ser Pro Asn Gly Ile Lys Ser Gly Ile Gly Ala 165 170 175 Gly Met Glu Val Leu Gly Val Arg His Gly Glu Asn Ala His Leu Asp 180 185 190 Leu Ser Ala Ser Ser His Val Phe Asp Asp Leu Tyr Gly Val Lys Glu 195 200 205 Tyr Leu Glu Thr Val Leu Asn Gly Asn Ala 210 215 <210> 116 <211> 222 <212> PRT <213> Metallosphaera sedula <400> 116 Met Trp Ile Val Ala Ser Asp Phe Asp Arg Thr Leu Ser His Glu Asp 1 5 10 15 Asp Ser Phe Val Met Arg Lys Glu Val Ala Ser Lys Ile Asn His Phe 20 25 30 Ser Thr Ile His Arg Phe Phe Val Val Thr Gly Arg Glu Glu Arg Tyr 35 40 45 Met Arg Val Leu Ala Pro Asp Leu Arg Pro Thr Gly Trp Val Leu Glu 50 55 60 Asn Gly Ala Leu Leu Ile Leu Gly Asp Arg Arg Ile Leu Asn Val Pro 65 70 75 80 Glu Asp Trp Phe Glu Thr Arg Lys Ile Ile Gly Glu Lys Leu Thr Lys 85 90 95 Phe Gly Ile Ser Tyr Ser Leu Gly Asp Val Ile Ile Tyr Val Asn Ser 100 105 110 Trp Asn Gly Lys Leu Asp Leu Gly Pro Glu Val Arg Ile Glu Arg Asn 115 120 125 Arg Glu Asp Ala Met Ile Leu Pro Gly Asn Val Asp Lys Gly Thr Gly 130 135 140 Leu Arg Arg Ala Ile Gln Glu Met His Leu Glu Gly Lys Ile Val Ala 145 150 155 160 Val Gly Asp Ala Glu Asn Asp Glu Ser Leu Phe Lys Val Ala Asp Val 165 170 175 Lys Val Ala Val Ala Asn Ala Ile Pro Pro Ile Lys Arg Met Ala Asp 180 185 190 Leu Val Met Glu Lys Glu Asp Gly Glu Gly Val Val Glu Leu Leu Asp 195 200 205 Met Ile Leu Ser Gly Arg Phe Pro Lys Asn Val Asp Val Asn 210 215 220 <210> 117 <211> 224 <212> PRT <213> Methanocella conradii <400> 117 Met Ile Lys Ala Val Val Ala Asp Ile Asp Gly Thr Leu Thr Asp Met 1 5 10 15 Gln Arg Arg Ile Ser Val Glu Ala Ile Val Ala Ile Arg Lys Leu Pro 20 25 30 Ile Pro Val Val Leu Ala Ser Gly Asn Val Ile Cys Phe Met Arg Ala 35 40 45 Ala Ser Lys Leu Ile Gly Ala Ser Glu Ala Met Ile Gly Glu Asn Gly 50 55 60 Gly Val Val Gln Val Gly Tyr Asp Ser Pro Pro Val Val Phe Ala Asp 65 70 75 80 Ile Glu Glu Cys Arg Arg Ala Ala Ala Leu Leu Gln Arg Arg Phe Pro 85 90 95 Gly Leu Glu Gln Leu Asp Ala Arg Tyr Arg Leu Ser Glu Leu Ala Phe 100 105 110 Arg Arg Thr Val Asp Ala Asn Glu Leu Arg Ala Ala Leu Asp Met Glu 115 120 125 Tyr Pro Ser Leu Glu Val Val Asp Thr Gln Phe Ala Leu His Leu Lys 130 135 140 His Lys Ser Val Asn Lys Gly Thr Gly Leu Val Asn Val Ala Ser Met 145 150 155 160 Met Gly Phe Lys Pro Glu Glu Phe Ala Ala Leu Gly Asp Ser Ala Asn 165 170 175 Asp Leu Pro Met Phe Glu Ala Ala Gly Leu Gly Leu Ala Val Gly Asn 180 185 190 Ala Val Pro Glu Leu Lys Asp Val Ala Asp Tyr Val Ala Val Lys Pro 195 200 205 Tyr Gly Glu Gly Ala Ala Glu Ala Leu Arg Trp Leu Ala Arg Gln Leu 210 215 220 <210> 118 <211> 237 <212> PRT <213> Methanocella conradii <400> 118 Met Ala Glu Arg Cys Ser Val Lys Ile Phe Leu Tyr Arg Ala Val Leu 1 5 10 15 Phe Asp Met Asp Gly Val Ile Ala Asp Thr Met Pro Leu His Tyr Glu 20 25 30 Ala Trp Arg Arg Ala Phe Glu Ala Phe Gly Val His Val Asp Lys Met 35 40 45 Asp Val Tyr Leu Arg Glu Gly Met Thr Thr Met Glu Met Gly Lys Asp 50 55 60 Ile Ala Arg Ser Lys Gly Met Glu Leu Ser Glu Gly Glu Leu Asn Ser 65 70 75 80 Ile Val Glu Leu Lys Thr Arg Ile Phe Asn Glu Leu Val Asp Ser Ser 85 90 95 Val Arg Leu Tyr Glu Gly Val Pro Glu Thr Leu Thr Met Leu Arg Asn 100 105 110 Asn Gly Met Lys Leu Ala Leu Val Thr Gly Ser Arg Arg Thr Ser Ala 115 120 125 Met Ala Val Leu Lys Lys Val Gly Leu Glu Gly Ala Phe Asp Ala Ile 130 135 140 Val Ala Ala Glu Asp Val Arg Arg Gly Lys Pro Asp Ala Glu Pro Tyr 145 150 155 160 Leu Val Ala Met Arg Ala Val Asp Val Pro Ala Leu Asn Cys Val Val 165 170 175 Val Glu Asn Ala Pro Leu Gly Ile Arg Ala Ala Arg Ala Ala Lys Val 180 185 190 Gly Tyr Ile Ile Ala Ile Ala Thr Thr Leu Asp Glu Ala His Leu Lys 195 200 205 Glu Ala Asp Glu Val Ala Pro Ser Phe Pro Glu Leu Glu Gln Cys Ile 210 215 220 Ala Arg Arg Phe Glu Ala Arg Pro Gly Arg Ala Ile Met 225 230 235 <210> 119 <211> 209 <212> PRT <213> Methanocella conradii <400> 119 Met Pro Arg Ile Arg Gly Leu Ile Phe Asp Leu Asp Gly Thr Leu Ile 1 5 10 15 Asp Asn Tyr Asp Gly Tyr Met Glu Leu Met Leu Arg Arg Val Gly Arg 20 25 30 Asp Leu Gly Arg Glu Phe Thr Leu Ser His Ala Lys Glu Leu Trp Tyr 35 40 45 Ser Ile Asn Ser Glu Ser Arg Asp Glu Val Ile Met Arg Trp Gly Val 50 55 60 Asn Pro Asp Arg Phe Trp Thr Val Phe Asn Arg Tyr Glu Asp Ile Asn 65 70 75 80 Glu Lys Leu Glu Asn Thr Tyr Leu His Asp Asp Ala Asp Ile Leu Lys 85 90 95 Gly Leu Asn Ile Pro Lys Gly Ile Val Thr His Thr Ser Tyr Glu His 100 105 110 Thr Asp Arg Leu Leu Gln Lys Val Gly Met Arg Gln Tyr Phe Arg Pro 115 120 125 Ile Ile Ala Cys Thr Glu Asp Thr Gly Tyr Lys Pro Ser Pro Leu Pro 130 135 140 Ile Ile Tyr Cys Val Val Gly Met Lys Leu Lys Pro Glu Glu Val Ala 145 150 155 160 Tyr Val Gly Asp Thr Leu Ser Asp Met Leu Ala Ala Arg Tyr Ala Gly 165 170 175 Val Lys Ser Ile Tyr Ile Asn Arg Tyr Asn Arg Pro Ile Lys Ala Ser 180 185 190 Pro Asp Tyr Glu Ile Lys Arg Met Asp Glu Leu Leu Glu Ile Leu Gly 195 200 205 Gln <210> 120 <211> 216 <212> PRT <213> Methanococcoides methylutens <400> 120 Met Leu Asn Ser Leu Ile Phe Asp Ala Asp Gly Val Leu Met Asp Ser 1 5 10 15 Met Pro Cys His Ala Asp Ala Trp Val His Thr Phe Ser Glu Val Gly 20 25 30 Ile Asp Ile Val Arg Gln Asp Ile Tyr Asp Ile Glu Gly Ser Asn His 35 40 45 Val Gly Val Ile Lys Leu Ile Phe Lys Lys Ala Gly Arg Glu Ala Asp 50 55 60 Pro Glu Ile Ile Glu Lys Leu Arg Val Arg Lys Arg Glu Leu Phe Leu 65 70 75 80 Lys Lys Lys Asn Ile Asp Pro Phe Asp Gly Met Tyr Asn Leu Leu Lys 85 90 95 Lys Leu Lys Asn Asp Phe His Leu Ala Val Val Ser Gly Ser Asp Arg 100 105 110 Pro Ile Val Asp Ser Met Ile Asn Glu Phe Tyr Pro Asp Ile Phe Glu 115 120 125 Val Thr Ile Ser Gly Ala Asp Val Asn Asn Gly Lys Pro Asp Pro Glu 130 135 140 Pro Tyr Leu Lys Ala Met Glu Ile Leu Lys Val Lys Lys Glu Asn Cys 145 150 155 160 Leu Val Ile Glu Asn Ala Pro Leu Gly Val Asp Ser Ala Lys Asn Ala 165 170 175 Gly Ile Tyr Cys Val Ala Val Pro Thr Tyr Val Asp Ala Glu Lys Leu 180 185 190 Lys Arg Ala Asp Gln Ile Ile Gln Asp His Lys Gln Leu Ile Glu Phe 195 200 205 Leu Ser Asp Ile Glu Ser Pro Arg 210 215 <210> 121 <211> 227 <212> PRT <213> Methanococcoides methylutens <400> 121 Met Val Phe Lys Ala Leu Val Val Asp Ile Asp Gly Thr Ile Thr Asn 1 5 10 15 Pro Asp Arg Ser Leu Asp Leu Arg Val Ala Lys Arg Phe Arg Glu Leu 20 25 30 Arg Val Pro Val Val Leu Ser Thr Gly Asn Pro Leu Cys Tyr Val His 35 40 45 Ala Ala Ala Lys Leu Ile Gly Leu Gly Gly Ile Val Ile Ala Glu Asn 50 55 60 Gly Gly Val Ile Ser Thr Gly Phe Asp Lys Pro Ser Ile Ile Ala Asp 65 70 75 80 Gly Met Glu Lys Cys Glu Glu Ala Tyr Glu Leu Leu Ser Asn Tyr Phe 85 90 95 Asp Leu Glu Lys Leu Asp Ala Ala Tyr Arg Lys Thr Glu Val Val Leu 100 105 110 Arg Arg Gly Met Asp Ile Ser Gln Leu Arg Glu Ile Val Glu Asp Asn 115 120 125 Gly Met Glu Val Glu Ile Ile Asp Thr Gly Tyr Ala Val His Ile Lys 130 135 140 Asp Gly Lys Ile Asn Lys Gly Thr Gly Leu His Thr Val Ala Glu Leu 145 150 155 160 Met Gly Ile Asp Thr Lys Asp Phe Leu Ala Ile Gly Asp Ser Cys Asn 165 170 175 Asp Ala Glu Met Met Arg Glu Ala Gly Leu Gly Ile Ala Val Gly Asn 180 185 190 Ala Asp Asp Asp Ala Arg Lys Ala Ala Ser Met Val Thr Lys Ala Ser 195 200 205 Phe Gly Glu Gly Met Leu Glu Ala Ile Glu Tyr Ala Phe Ser Asn Gly 210 215 220 Leu Leu Glu 225 <210> 122 <211> 234 <212> PRT <213> Methanohalobium evestigatum <400> 122 Met Lys Tyr Lys Ala Leu Val Ala Asp Ile Asp Gly Thr Ile Thr His 1 5 10 15 Gln Asn Arg Arg Leu Asn Leu Lys Ala Ala Glu Met Leu Tyr Arg Leu 20 25 30 Asn Asp Glu Ser Gly Ile Pro Val Val Leu Ala Thr Gly Asn Ile Leu 35 40 45 Cys Phe Ser His Ala Ala Ser Lys Leu Ile Gly Leu Ser Gly Tyr Val 50 55 60 Ile Ala Glu Asn Gly Gly Val Val Ser Glu Gly Phe Asp Thr Asn Pro 65 70 75 80 Tyr Ile Met Gly Asp Ile Lys Glu Cys Arg Lys Ala Phe Ser Phe Leu 85 90 95 Ser Glu Tyr Ile Pro Leu Thr Lys Leu Asp Pro Glu Tyr Arg Lys Thr 100 105 110 Glu Ile Ala Leu Arg Arg Asn Phe Asp Leu Glu Tyr Ala Lys Asn Val 115 120 125 Ile Lys Ser Gly Lys Tyr Asn Val Asp Leu Ile Asp Thr Gly Tyr Ala 130 135 140 Val His Ile Lys Ser Asn Thr Met Asn Lys Gly Thr Gly Leu Val Lys 145 150 155 160 Ile Ala Glu Leu Met Gly Leu Asn Pro Lys Glu Phe Val Ala Ile Gly 165 170 175 Asp Ser Glu Asn Asp Leu Glu Met Ile Glu Phe Ala Gly Ile Gly Ile 180 185 190 Ala Val Ala Asn Ser Asp Gln Lys Thr Arg Ser Ile Ala Asp Ile Val 195 200 205 Thr Asp Ala Ser Tyr Gly Glu Gly Val Val Glu Ala Ile Glu Arg Leu 210 215 220 Tyr Ser Cys Asp Trp Asn Asp Thr Cys Leu 225 230 <210> 123 <211> 217 <212> PRT <213> Methanohalobium evestigatum <400> 123 Met Leu Lys Ala Ile Ile Phe Asp Phe Asp Gly Val Leu Ala Asp Ser 1 5 10 15 Met Pro Tyr His Ala Gln Ala Trp Lys Ile Ala Phe Glu Lys Ala Gly 20 25 30 Ile Glu Ile Asn Lys Asp Asp Ile Tyr Glu Ile Glu Gly Ser Asn His 35 40 45 Val Gly Ile Ile Gln Leu Met Phe Gln Lys Met Gly Lys Ser Pro Gln 50 55 60 Pro His Gln Phe Asp Glu Ile Ala Gln Glu Lys Arg Lys Ile Phe Ser 65 70 75 80 Gln Ile Ala Asn Leu Lys Asn Phe Glu Asp Met Asp Lys Cys Leu Ser 85 90 95 Gln Leu Lys Asn Lys Phe Lys Leu Ala Val Val Ser Gly Ala Asp Arg 100 105 110 Asn Ala Val Phe Asn Met Val Gly Arg Phe Tyr Pro Asp Val Phe Asp 115 120 125 Val Ile Val Thr Gly Glu Asp Val Asp Asn Gly Lys Pro Ser Pro Glu 130 135 140 Pro Tyr Leu Lys Ala Leu Gly Met Leu Asn Val Glu Lys Asn Glu Cys 145 150 155 160 Leu Val Val Glu Asn Ala Pro Leu Gly Val Asp Ser Ala Lys Asn Ala 165 170 175 Gly Leu Tyr Cys Val Ala Val Pro Thr Tyr Val Asp Pro Glu Lys Leu 180 185 190 Asp Lys Ala Asp Val Val Val Glu Asp His Ser Asp Leu Lys Lys Tyr 195 200 205 Leu Gln Lys Ile Tyr Ser Glu Gln Ala 210 215 <210> 124 <211> 226 <212> PRT <213> Methanolobus tindarius <400> 124 Met Lys Phe Lys Ala Ile Val Ile Asp Ile Asp Gly Thr Ile Thr His 1 5 10 15 Arg Asp Arg Lys Leu Ser Leu Thr Ala Ala Ala Lys Leu Arg Ala Leu 20 25 30 Asp Ile Pro Val Val Ile Ala Thr Gly Asn Val Leu Cys Tyr Ala Lys 35 40 45 Ala Thr Ala Lys Leu Val Gly Val Cys Cys Asn Ile Ile Ala Glu Asn 50 55 60 Gly Gly Val Ile Ile Asp Gly Phe Asp Asn Asp Pro Ile Val Ser Asp 65 70 75 80 Val Ile His Glu Cys Glu Glu Ala Tyr Glu Phe Leu Ser Ser Glu Phe 85 90 95 Asn Met Glu Lys Leu Asp Ser Val His Arg Arg Thr Glu Ile Val Leu 100 105 110 Arg Ser Asn Phe Asp Val Glu Lys Ala Arg Glu Val Leu Gly Thr His 115 120 125 Phe Pro Asn Val Glu Ile Ile Asp Thr His Phe Ala Ile His Ile Lys 130 135 140 Ser Lys Lys Ile Asn Lys Gly Thr Gly Leu Phe Lys Met Ala Glu Met 145 150 155 160 Met Gly Leu Glu Thr Thr Asp Phe Val Ala Ile Gly Asp Ser Ile Asn 165 170 175 Asp Leu Glu Met Leu Gln Glu Ala Gly Phe Ala Val Ala Val Gly Asn 180 185 190 Ala Asp Asp Phe Leu Lys Asp Ile Ala Asp Tyr Val Ser Glu Ala Ser 195 200 205 Tyr Gly Asp Gly Thr Val Glu Ala Ile Asp Tyr Leu Ile Ser Ser Gly 210 215 220 Leu Ile 225 <210> 125 <211> 217 <212> PRT <213> Methanolobus tindarius <400> 125 Met Ile Lys Gly Ile Ile Phe Asp Ser Asp Gly Val Leu Val Asn Ser 1 5 10 15 Met Pro Phe His Ala Lys Ala Trp Val Glu Val Phe Ala Glu Tyr Gly 20 25 30 Ile Glu Val Thr Glu Glu Asp Ile Tyr Glu Ile Glu Gly Ser Asn His 35 40 45 Val Gly Val Ile Asn Ile Phe Phe Gly Lys Ala Gly Arg Thr Pro Glu 50 55 60 Pro Glu Ile Tyr Ala Glu Ile Leu Glu Lys Lys Arg Ala His Phe Leu 65 70 75 80 Glu Asn Asn Arg Ala Glu Val Phe Glu Gly Met Tyr Asp Cys Leu Ser 85 90 95 Ser Leu Lys Asn Lys Phe Lys Leu Ala Val Ala Ser Gly Ala Asp Arg 100 105 110 Thr Ile Val Thr Ser Leu Met Asp Lys Phe Tyr Pro Gly Val Phe Asp 115 120 125 Ala Ile Ile Ser Gly Glu Asp Val Glu Asn Gly Lys Pro Asp Pro Glu 130 135 140 Pro Tyr Glu Lys Ala Ile Val Lys Leu Gly Leu Ser Lys Asp Glu Cys 145 150 155 160 Leu Val Val Glu Asn Ala Pro Leu Gly Val Glu Ser Ala Lys Asn Ala 165 170 175 Gly Val Phe Cys Val Gly Val Pro Thr Tyr Leu Asp Glu Ser Lys Leu 180 185 190 Lys Glu Ala Asp Phe Val Val Arg Asn His Ser Glu Leu Ile Glu Tyr 195 200 205 Leu Thr Asp Leu Lys Asp Ser Ser Leu 210 215 <210> 126 <211> 228 <212> PRT <213> Methanolobus tindarius <400> 126 Met Leu Met Lys Phe Lys Ala Ile Val Ile Asp Ile Asp Gly Thr Ile 1 5 10 15 Thr His Arg Asp Arg Lys Leu Ser Leu Thr Ala Ala Ala Lys Leu Arg 20 25 30 Ala Leu Asp Ile Pro Val Val Ile Ala Thr Gly Asn Val Leu Cys Tyr 35 40 45 Ala Lys Ala Thr Ala Lys Leu Val Gly Val Cys Cys Asn Ile Ile Ala 50 55 60 Glu Asn Gly Gly Val Ile Ile Asp Gly Phe Asp Asn Asp Pro Ile Val 65 70 75 80 Ser Asp Val Ile His Glu Cys Glu Glu Ala Tyr Glu Phe Leu Ser Ser 85 90 95 Glu Phe Asn Met Glu Lys Leu Asp Ser Val His Arg Arg Thr Glu Ile 100 105 110 Val Leu Arg Ser Asn Phe Asp Val Glu Lys Ala Arg Glu Val Leu Gly 115 120 125 Thr His Phe Pro Asn Val Glu Ile Ile Asp Thr His Phe Ala Ile His 130 135 140 Ile Lys Ser Lys Lys Ile Asn Lys Gly Thr Gly Leu Phe Lys Met Ala 145 150 155 160 Glu Met Met Gly Leu Glu Thr Thr Asp Phe Val Ala Ile Gly Asp Ser 165 170 175 Ile Asn Asp Leu Glu Met Leu Gln Glu Ala Gly Phe Ala Val Ala Val 180 185 190 Gly Asn Ala Asp Asp Phe Leu Lys Asp Ile Ala Asp Tyr Val Ser Glu 195 200 205 Ala Ser Tyr Gly Asp Gly Thr Val Glu Ala Ile Asp Tyr Leu Ile Ser 210 215 220 Ser Gly Leu Ile 225 <210> 127 <211> 218 <212> PRT <213> Methanosarcina siciliae <400> 127 Met Leu Lys Ala Ile Ile Phe Asp Val Asp Gly Val Leu Val Asp Ser 1 5 10 15 Met Arg Phe Gln Ala Glu Ala Trp Val Lys Thr Leu Lys Glu Val Gly 20 25 30 Val Thr Val Ser Arg Glu Asp Ile Tyr Glu Leu Glu Gly Ser Asn Asn 35 40 45 Leu Gly Leu Ile Lys Leu Val Phe Glu Lys Ala Gly Lys Glu Pro Glu 50 55 60 Pro Trp His Tyr Glu Gln Leu Pro Val Lys Lys Arg Glu Val Leu Glu 65 70 75 80 Phe Asp Arg Ile Lys Pro Phe Glu Gly Met Pro Ser Cys Leu Asn Gln 85 90 95 Leu Lys Arg His Phe Arg Leu Ala Met Val Ser Gly Ser Asn Phe Asp 100 105 110 Ile Val Ser Thr Phe Ala Asn Ser Phe Phe Pro Asp Cys Phe Glu Val 115 120 125 Ile Val Thr Gly Gly Asp Leu Glu Arg Gly Lys Pro Cys Pro Asp Pro 130 135 140 Tyr Ile Lys Ala Leu Glu Lys Leu Asp Leu Ala Thr Asn Glu Cys Ile 145 150 155 160 Val Val Glu Asn Ala Pro Leu Gly Ile Thr Ala Ala Lys Arg Ala Gly 165 170 175 Leu Tyr Cys Val Ala Val Ala Ser Met Leu Ser Pro Glu Lys Val Lys 180 185 190 His Ala Asp Ile Val Phe Glu Asn His Ala Val Leu Leu Asp Tyr Leu 195 200 205 Lys Ser Leu Ile Pro Gly Glu Ser His Thr 210 215 <210> 128 <211> 253 <212> PRT <213> Methanosarcina siciliae <400> 128 Met Ser Ser Ile Ile Asn Tyr Gly Ser Ile Ile Asn Tyr Glu Trp Gly 1 5 10 15 Ser Ile Leu Ser Pro Leu Ser His Leu Phe Ser Met Lys Phe Lys Ala 20 25 30 Ile Val Val Asp Ile Asp Gly Thr Ile Thr Cys Glu Asn Arg Glu Leu 35 40 45 His Leu Gly Ala Val Lys Lys Ile Arg Ser Leu Lys Val Pro Val Val 50 55 60 Leu Ala Thr Gly Asn Ile Ile Cys Tyr Ala Arg Thr Ala Ser Lys Leu 65 70 75 80 Ile Gly Leu Glu Gly Ala Val Val Ala Glu Asn Gly Gly Ala Val Thr 85 90 95 Val Arg Tyr Asp Leu Asn Gly Thr Phe Glu Glu Ser Leu Glu Glu Cys 100 105 110 Glu Lys Ala Phe Ser Phe Leu Ser Glu His Phe Gln Leu Thr Lys Leu 115 120 125 Asp Pro Phe Tyr Arg Lys Thr Glu Ile Ala Leu Arg Arg Asp Phe Asp 130 135 140 Leu Glu Lys Ala Arg Ala Leu Leu Lys Thr Gln Leu Leu Asp Val Glu 145 150 155 160 Met Val Asp Thr Lys Tyr Ala Val His Ile Lys Ser Thr Arg Ile Asn 165 170 175 Lys Gly Ser Gly Leu Arg Lys Leu Ala Glu Met Met Gly Leu Glu Ala 180 185 190 Lys Asp Phe Val Ala Ile Gly Asp Ser Glu Asn Asp Val Glu Met Phe 195 200 205 Lys Ala Ala Gly Phe Gly Ile Ala Val Ala Asn Gly Asp Thr Lys Ile 210 215 220 Lys Glu Ala Ala Asp Tyr Val Thr Glu Ala Ser Phe Gly Lys Gly Ala 225 230 235 240 Val Glu Ala Phe Glu Tyr Leu Glu Ser Lys Gly Leu Ile 245 250 <210> 129 <211> 213 <212> PRT <213> Methanosarcina siciliae <400> 129 Met Lys Tyr Asn Thr Val Ile Phe Asp Phe Asp Tyr Thr Leu Ala Asp 1 5 10 15 Ala Thr Asp Gly Ile Val Ser Ser Phe Asn His Ala Phe Ser Glu Leu 20 25 30 Asp Leu Ala Ser Tyr Asp Ile Glu Ser Ile Lys Arg Thr Val Gly Leu 35 40 45 Ser Leu Asp Glu Ala Phe Val Gln Leu Thr Ala Asn Glu Asp Lys Ala 50 55 60 Leu Arg Asn Arg Phe Lys Ile Leu Phe Lys Glu Lys Ala Asp Glu Val 65 70 75 80 Met Ser Lys Asn Thr Val Leu Phe Asp Asp Thr Ile Ser Thr Leu Arg 85 90 95 Gln Leu Lys Arg Asp Gly Leu Asn Thr Gly Ile Val Thr Thr Lys Tyr 100 105 110 His Tyr Arg Ile Met Glu Thr Leu Asn Thr Tyr Gly Ile Ser Asp Leu 115 120 125 Val Asp Ile Ile Val Gly Gly Glu Asp Val Lys Ala Pro Lys Pro Ser 130 135 140 Pro Glu Gly Leu Leu Leu Ala Ile Asp Ser Leu Asn Ser Arg Gln Asn 145 150 155 160 Asn Val Leu Tyr Ile Gly Asp Ser Leu Val Asp Ala Lys Thr Ala Leu 165 170 175 Ala Ala Asn Val Asp Phe Val Ala Val Thr Thr Gly Thr Thr Glu Val 180 185 190 Lys Glu Phe Ser Gln Tyr Pro Cys Ile Arg Ile Leu Lys Asn Leu Ser 195 200 205 Glu Leu Phe Asp Arg 210 <210> 130 <211> 224 <212> PRT <213> Methanothermus fervidus <400> 130 Met Lys Ile Glu Ala Ile Ala Val Asp Ile Asp Gly Thr Ile Thr Asp 1 5 10 15 Thr Lys Arg Arg Leu Cys Ile Asp Ala Ile Lys Ala Leu Arg Asp Ala 20 25 30 Glu Lys Leu Gly Ile Lys Val Thr Leu Val Thr Gly Asn Ile Leu Cys 35 40 45 Tyr Ala Tyr Ala Thr Ser Thr Leu Ile Gly Leu Ser Gly Gly Val Val 50 55 60 Ala Glu Asn Gly Gly Val Ile Tyr Val Asn Asp Glu Val Asn Val Leu 65 70 75 80 Gly Asp Ile Lys Lys Ala Lys Lys Ala Tyr Asn Tyr Leu Lys Ser Ile 85 90 95 Tyr Pro Val Glu Lys Val Gln Phe Ser Glu Leu Arg Val Ser Glu Ile 100 105 110 Ala Ile Lys Arg Thr Ile Pro Val Glu Ile Ile Lys Asp Ala Leu Lys 115 120 125 Asp Phe Asp Val Glu Val Tyr Asp Thr Lys Phe Ala Ile His Leu Thr 130 135 140 Asp Pro Lys Val Asp Lys Gly Ser Ser Leu Lys Ile Leu Thr Lys Lys 145 150 155 160 Leu Gly Ile Asp Leu Glu Lys Thr Ile Ala Ile Gly Asp Ser Glu Asn 165 170 175 Asp Leu Glu Phe Leu Asp Val Ala Gly Val Lys Val Ala Val Ala Asn 180 185 190 Ala Asn Lys Glu Leu Lys Glu Ile Ala Asp Tyr Val Thr Lys Lys Pro 195 200 205 Tyr Gly Ser Gly Val Ala Glu Ala Ile Lys Lys Tyr Val Thr Leu Ser 210 215 220 <210> 131 <211> 277 <212> PRT <213> Petrotoga mobilis <400> 131 Met Lys Lys Thr Phe Val Phe Asp Leu Asp Gly Thr Leu Leu Asn Ser 1 5 10 15 Glu Val Arg Ile Ser Pro Lys Thr Tyr Gln Ala Leu Lys Arg Leu Lys 20 25 30 Glu Glu Gly His Ile Ile Ile Ile Ala Ser Gly Arg Met Tyr Ala Ser 35 40 45 Thr Met Tyr Val Val Glu Asn Phe Leu Pro Phe Leu Lys Gly Asn Val 50 55 60 Ile Ile Ser Ser Tyr Asn Gly Gly Tyr Ile Val Asp His Asn Gly Lys 65 70 75 80 Val Val Phe Glu Lys Gly Val Ala Asn Glu Ser Ala Ile Lys Cys Ile 85 90 95 Lys Phe Leu Arg Asp Leu Asn Ile His Arg His Ile Tyr Ile Asn Asp 100 105 110 Lys Leu Ile Ser Glu Ile Asp Asp Lys Glu Ile Arg Asp Tyr Ser Lys 115 120 125 His Ser Phe Val Asp Tyr Val Leu Val Asp Asp Leu Ile Ala Glu Ile 130 135 140 Glu Thr Ser Ser His Pro Thr Leu Lys Ile Leu Ala Ile Gly Glu Pro 145 150 155 160 Asp Lys Ile Asp Val Ile Lys Lys Leu Ala Glu Asn Glu Leu Gln Gly 165 170 175 Glu Phe Asn Leu Met Lys Ser Trp Asp Thr Tyr Leu Asp Phe Ile Pro 180 185 190 Tyr Gly Val Ser Lys Gly Asn Ser Leu Lys Ile Ile Ser Lys Ile Tyr 195 200 205 Asn Leu Asp Pro Asn Thr Leu Tyr Val Phe Gly Asp Ser Glu Asn Asp 210 215 220 Ile Asp Met Leu Glu Leu Thr Lys Asn Ser Phe Ala Met Gly Asn Ala 225 230 235 240 Lys Glu Asp Val Lys Lys Val Ala Asn Tyr Leu Leu Pro Ser Asn Asp 245 250 255 Glu Asp Gly Val Ala Tyr Ala Ile Glu Lys Ile Leu Ala Asp Asp Leu 260 265 270 Asp Asn Val Asn Ile 275 <210> 132 <211> 214 <212> PRT <213> Petrotoga mobilis <400> 132 Met Ile Lys Ala Cys Ile Phe Asp Leu Asp Gly Val Ile Val Asp Thr 1 5 10 15 Ala Lys Tyr His Tyr Met Ala Trp Lys Arg Leu Ala Asp Glu Leu Asn 20 25 30 Ile Pro Phe Asn Glu Lys Asp Asn Glu Arg Leu Lys Gly Val Ser Arg 35 40 45 Met Lys Ser Leu Glu Ile Ile Leu Asp Leu Gly Asn Val Asn Leu Ser 50 55 60 Gln Glu Glu Lys Glu Glu Leu Ala Gln Lys Lys Asn Asn Trp Tyr Val 65 70 75 80 Glu Cys Ile Ser Asn Met Asp Lys Ser Glu Leu Leu Pro Gly Val Glu 85 90 95 Glu Phe Ile Lys Asn Leu Lys Arg Lys Gly Ile Lys Val Ala Ile Ala 100 105 110 Ser Ala Ser Lys Asn Thr Lys Leu Ile Leu Lys Lys Leu Asn Leu Glu 115 120 125 Asp Thr Phe Asp Ala Val Ile Asp Gly Thr Met Ile Asp Lys Ala Lys 130 135 140 Pro Asn Pro Glu Ile Phe Leu Lys Ala Ser Asp Tyr Leu Asn Val Lys 145 150 155 160 Pro Glu Glu Cys Leu Val Phe Glu Asp Ala Val Ala Gly Val Gln Ala 165 170 175 Ala Lys Lys Ala Gly Met Lys Val Ile Gly Val Gly Glu Lys Lys Val 180 185 190 Leu Lys Gly Ala Asp Lys Val Ile Lys Asn Phe Glu Asn Val Asp Leu 195 200 205 Thr Leu Ile Glu Gly Ile 210 <210> 133 <211> 236 <212> PRT <213> Picrophilus torridus <400> 133 Met Phe Met Thr Lys Asp Leu Phe Phe Val Tyr Ile Phe Thr Thr Met 1 5 10 15 Ile Lys Leu Val Val Leu Asp Val Asp Gly Thr Leu Thr Asp Lys Ser 20 25 30 Arg Met Ile Ser Val Asn Ala Val Asn Ala Ile Arg Asn Leu Lys Thr 35 40 45 Lys Val Ala Leu Val Ser Gly Asn Val Leu Pro Val Leu Tyr Gly Leu 50 55 60 Lys Ile Tyr Ile Gly Phe Asp Gly Tyr Ile Phe Ala Glu Asn Gly Gly 65 70 75 80 Ile Ala Leu Ile Asn Asn Asn Ile Glu Lys Phe Phe Glu Lys Asp Gly 85 90 95 Pro Glu Ser Phe Leu Asn Asp Ile Ser Gly Tyr Thr Ser Ala Arg Gly 100 105 110 Ile Leu Thr Asn Arg Trp Arg Glu Thr Ser Met Ala Phe Thr Ala Asn 115 120 125 His Asp Glu Met Asp Ile Ile Asp Arg Glu Ala Ala Ser Arg Asp Leu 130 135 140 Tyr Ile Val Asp Ser Gly Phe Thr Leu His Ile Leu Asn Lys Gly Gln 145 150 155 160 Asp Lys Gly Phe Ala Val Lys Lys Met Ile Asp Ile Met Asn Ile Asp 165 170 175 Tyr Asn Asn Val Leu Val Ile Gly Asp Ser Gln Asn Asp Glu Ser Met 180 185 190 Phe Ser Leu Gly Thr Leu Ser Ala Cys Pro Gly Asn Ala Ser Glu Lys 195 200 205 Ile Lys Glu Met Ser Asn Tyr Val Ser Gly Lys Cys Tyr Gly Asp Glu 210 215 220 Leu Phe Asp Val Phe Arg His Phe Asp Leu Ile His 225 230 235 <210> 134 <211> 202 <212> PRT <213> Picrophilus torridus <400> 134 Met Phe Lys Ala Val Ile Phe Asp Leu Asp Gly Thr Ile Leu Asp Ser 1 5 10 15 Ile Pro Leu Arg Val Arg Ser Trp Gln Gln Ala Phe Asp Asp Tyr Asn 20 25 30 Ile Ser Ala Asp Pro Glu Ile Ile Arg Leu Met Ile Gly Tyr Pro Gly 35 40 45 Ser Met Leu Ile Lys Lys Val Asn Ala Met Asn Pro Glu Ile Glu Phe 50 55 60 Arg Glu Glu Tyr Tyr Phe Lys Met His Leu Asp Glu Leu Arg Phe Phe 65 70 75 80 Pro Asp Val Asp Asp Thr Phe Lys Glu Leu Arg Lys Leu Gly Val Lys 85 90 95 Ile Ala Val Val Thr Ser Ser Arg Lys Asp Phe Val Met Arg Leu Asn 100 105 110 Ile Lys Ala Asp Ala Ile Val Thr Ile Asp Asp Val Lys Asn Gly Lys 115 120 125 Pro Asp Thr Glu Pro Tyr Ile Lys Ala Met Ser Met Met Asn Val Lys 130 135 140 Pro Asp Glu Thr Val Val Val Gly Asp Ile Asp Asn Asp Leu Ile Pro 145 150 155 160 Ser Arg Glu Leu Gly Cys Leu Ser Val Leu Val Arg His Asn Arg Asn 165 170 175 Val Ser Ser Asp His Ala Asp Ile Ile Ile Glu Glu Ile Lys Glu Val 180 185 190 Leu Asn Ile Phe Asn Asp Lys Lys Asp Leu 195 200 <210> 135 <211> 228 <212> PRT <213> Pseudonocardia thermophila <400> 135 Met Thr Asp Arg Ala Asp Thr Ala His Pro Asp Thr Ala His Pro Asp 1 5 10 15 Thr Ala Ile Val Asp Val Asp Gly Thr Leu Val Asp Thr Asn Tyr His 20 25 30 His Ala Leu Ala Trp Phe Arg Ala Phe Arg Arg Phe Gly Val Thr Leu 35 40 45 Pro Val Trp Arg Leu His Arg Ala Ile Gly Met Gly Gly Asp Gln Leu 50 55 60 Val Pro Ala Val Ala Gly Asn Arg Phe Glu Asp Asp His Gly Asp Ala 65 70 75 80 Val Arg Glu Ala Trp Lys Asp Glu Phe Asp Pro Leu Met Gly Glu Val 85 90 95 Arg Pro Phe Gly Gly Val Cys Glu Leu Leu Thr Ala Phe Gly Glu Ser 100 105 110 Gly Leu Lys Val Val Leu Ala Ser Ser Gly Ala Pro Glu His Val Asp 115 120 125 Ala Tyr Leu Asp Leu Phe Asp Gly Arg Arg Leu Ala Asp Ala Trp Thr 130 135 140 Thr Ser Glu Asp Val Asp Arg Thr Lys Pro Glu Pro Asp Leu Ile Thr 145 150 155 160 Ala Ala Leu Asp Arg Val Arg Gly Glu Val Gly Ile Val Ile Gly Asp 165 170 175 Ser Val Trp Asp Phe Arg Ala Ala Glu Arg Ala Gly Gln Arg Gly Tyr 180 185 190 Ala Ile Arg Thr Gly Gly Phe Ser Glu Ser Glu Leu Arg Glu Ala Gly 195 200 205 Ala Arg Asn Val Phe Glu Ser Leu Pro Glu Leu His Gln Ala Leu Pro 210 215 220 Arg Ile Leu Gly 225 <210> 136 <211> 273 <212> PRT <213> Pyrococcus furiosus <400> 136 Met Glu Met Arg Ile Val Phe Asp Met Asp Gly Val Leu Tyr Arg Gly 1 5 10 15 Asn Thr Pro Ile Glu Gly Ala Arg Glu Val Ile Glu Phe Leu Lys Glu 20 25 30 Lys Gly Ile Lys Phe Ala Phe Leu Thr Asn Asn Ser Thr Lys Thr Pro 35 40 45 Glu Met Tyr Arg Glu Arg Leu Leu Lys Met Gly Ile Asp Val Pro Ala 50 55 60 Asp Ser Ile Ile Thr Ser Gly Leu Ala Thr Arg Ile Tyr Met Lys Lys 65 70 75 80 His Phe Glu Pro Gly Lys Ile Phe Val Ile Gly Gly Arg Gly Leu Val 85 90 95 Glu Glu Met Glu Lys Leu Gly Trp Gly Ile Val Ser Val Glu Glu Ala 100 105 110 Arg Glu Gly Ile Trp Lys Glu Val Lys Tyr Val Val Val Gly Leu Asp 115 120 125 Pro Glu Leu Thr Tyr Glu Lys Leu Lys Tyr Gly Thr Leu Ala Ile Arg 130 135 140 Asn Gly Ala Glu Phe Ile Gly Thr Asn Pro Asp Arg Thr Tyr Pro Gly 145 150 155 160 Glu Glu Gly Ile Tyr Pro Gly Ala Gly Ser Ile Ile Ala Ala Leu Glu 165 170 175 Ala Ala Thr Asp Lys Lys Pro Leu Ile Ile Gly Lys Pro Asn Glu Pro 180 185 190 Met Tyr Glu Val Leu Arg Glu Lys Leu Gly Glu Gly Glu Val Trp Met 195 200 205 Val Gly Asp Arg Leu Asp Thr Asp Ile Leu Phe Ala Lys Lys Phe Gly 210 215 220 Met Lys Ala Ile Met Val Leu Thr Gly Val His Ser Leu Ser Asp Ile 225 230 235 240 Glu Lys Ser Asp Ile Lys Pro Asp Leu Val Leu Pro Ser Ile Lys Glu 245 250 255 Leu Leu Glu Tyr Leu Lys Ile Leu His Asp Glu Asp Lys Glu Gly Thr 260 265 270 Lys <210> 137 <211> 351 <212> PRT <213> Pyrococcus furiosus <400> 137 Met Ser Lys Pro Leu Phe Val Thr Pro Pro Tyr Val Ser Thr Leu Leu 1 5 10 15 Thr Leu Ile Glu Tyr Cys Thr Trp Leu Ile Ser Cys Pro Ser Leu Lys 20 25 30 Phe Pro Pro Pro Cys Phe Ile Met Pro Thr Phe Trp Ala Gly Tyr Phe 35 40 45 Cys Cys Trp Lys Ser Arg Asn Phe Leu Phe Asn Tyr Asn Tyr Ser Phe 50 55 60 Ile Arg Pro Met Leu Asp Phe Phe Cys Ser Ser Ile Cys Phe Gly Val 65 70 75 80 Leu Arg Ile Tyr Arg Phe Leu Ile Thr Thr Phe Ser Thr Asp Lys Asp 85 90 95 Ser Ser Pro Pro Val Gly Tyr His Asn Thr Pro Thr Phe Gly Ala Glu 100 105 110 Phe His His Leu Ser His Pro His Thr Ser Gln Gly Gln Ser Leu Ile 115 120 125 Val Gln Asn Lys Lys Phe Thr Leu His His Ser Leu Glu Gly Arg Cys 130 135 140 Met Lys Ile Ser Lys Ile Ile Glu Gln Ile Lys Ser Glu Leu Asp Lys 145 150 155 160 Lys Asp Lys Leu Arg Glu Glu Ala Leu Glu Ile Thr Arg Asp Ile Ile 165 170 175 Arg Leu Ser Gly Asp Ala Ile Lys Ala Met His Arg Gly Glu Leu Glu 180 185 190 Leu Ala His Glu Arg Leu Glu Lys Ala Arg Gly Leu Val Lys Glu Leu 195 200 205 Lys Glu Lys Leu Arg Glu His Pro Asp Leu Tyr Tyr Thr Gly Tyr Val 210 215 220 Gln Asn Ala Asn Gln Glu Phe Val Glu Ala Val Leu Met Tyr His Tyr 225 230 235 240 Leu Thr Asp Arg Glu Phe Pro Ser His Val Asp Leu Gly Val Pro Ser 245 250 255 Gln Asp Tyr Ile Leu Gly Val Gly Asp Phe Ile Gly Glu Leu Arg Arg 260 265 270 Tyr Phe Leu Ile Asn Leu Met Lys Gly Asn Leu Asp Glu Ala Glu Ser 275 280 285 Thr Tyr Arg Phe Met Glu Glu Val Tyr Glu Glu Leu Met Thr Leu Glu 290 295 300 Tyr Pro Lys Gly Leu Val Asn Ile Arg Gln Lys Gln Asp Gln Ala Arg 305 310 315 320 Tyr Thr Leu Glu Arg Thr Leu Glu Asp Leu Thr Arg Ala Lys Val Asn 325 330 335 Arg Arg Val Glu Glu Lys Leu Glu Ala Phe Leu Asn Asp Arg Ser 340 345 350 <210> 138 <211> 207 <212> PRT <213> Pyrococcus furiosus <400> 138 Met Lys Ile Ser Lys Ile Ile Glu Gln Ile Lys Ser Glu Leu Asp Lys 1 5 10 15 Lys Asp Lys Leu Arg Glu Glu Ala Leu Glu Ile Thr Arg Asp Ile Ile 20 25 30 Arg Leu Ser Gly Asp Ala Ile Lys Ala Met His Arg Gly Glu Leu Glu 35 40 45 Leu Ala His Glu Arg Leu Glu Lys Ala Arg Gly Leu Val Lys Glu Leu 50 55 60 Lys Glu Lys Leu Arg Glu His Pro Asp Leu Tyr Tyr Thr Gly Tyr Val 65 70 75 80 Gln Asn Ala Asn Gln Glu Phe Val Glu Ala Val Leu Met Tyr His Tyr 85 90 95 Leu Thr Asp Arg Glu Phe Pro Ser His Val Asp Leu Gly Val Pro Ser 100 105 110 Gln Asp Tyr Ile Leu Gly Val Gly Asp Phe Ile Gly Glu Leu Arg Arg 115 120 125 Tyr Phe Leu Ile Asn Leu Met Lys Gly Asn Leu Asp Glu Ala Glu Ser 130 135 140 Thr Tyr Arg Phe Met Glu Glu Val Tyr Glu Glu Leu Met Thr Leu Glu 145 150 155 160 Tyr Pro Lys Gly Leu Val Asn Ile Arg Gln Lys Gln Asp Gln Ala Arg 165 170 175 Tyr Thr Leu Glu Arg Thr Leu Glu Asp Leu Thr Arg Ala Lys Val Asn 180 185 190 Arg Arg Val Glu Glu Lys Leu Glu Ala Phe Leu Asn Asp Arg Ser 195 200 205 <210> 139 <211> 204 <212> PRT <213> Pyrococcus furiosus <400> 139 Met Lys Gly Leu Ile Ala Phe Asp Leu Glu Gly Thr Leu Thr Asp Met 1 5 10 15 Ile Ser Trp Glu Ile Leu His Glu Lys Phe Gly Thr Cys Asp Lys Ala 20 25 30 Arg Val His Thr Ser Leu Phe Leu Ser Gly Lys Ile Thr Tyr His Glu 35 40 45 Trp Ala Glu Met Asp Val Arg Leu Trp Lys Gly Arg Arg Arg Glu Glu 50 55 60 Val Glu Glu Ala Phe Ser Gln Val Thr Leu Lys Pro Tyr Ala Arg Glu 65 70 75 80 Leu Phe Glu Trp Leu Lys Lys Asn Asn Phe Lys Thr Ala Ile Ile Ser 85 90 95 Gly Gly Leu Met Cys Leu Ala Arg Lys Val Gly Glu Lys Leu Gly Val 100 105 110 Asp Phe Ile Val Ala Asn Glu Leu Lys Phe Asp Ser Gln Gly Arg Ile 115 120 125 Glu Gly Val Ile Val Arg Val Thr Phe Asp Asn Lys Gly Glu Ile Leu 130 135 140 Arg Gln Leu Lys Gln Lys Val Asn Pro Asn Val Thr Ile Ala Val Gly 145 150 155 160 Asp Trp Lys Asn Asp Lys Ser Met Phe Glu Glu Ala Asp Ile Ser Ile 165 170 175 Ser Leu Gly Asp Ile Asp Gly Asp Tyr Lys Ala Arg Asp Leu Arg Asp 180 185 190 Val Leu Glu Ile Leu Lys Ser Ile Val Arg Asn Ile 195 200 <210> 140 <211> 235 <212> PRT <213> Pyrococcus furiosus <400> 140 Met Ala Ile Thr Leu Lys Leu Leu Gln Met Cys Ser Ser Leu Met Ile 1 5 10 15 Ile Ala Phe Asp Phe Asp Gly Thr Leu Val Asp Ser Tyr Ser Cys Ile 20 25 30 Glu Glu Ala Phe Tyr Arg Ala Leu Lys Arg Thr Tyr Pro Trp Leu Pro 35 40 45 Gly Lys Arg Tyr Ile Ala Lys Leu Leu Thr Lys Leu Glu Leu Gln Phe 50 55 60 Glu Arg Pro Lys Phe Gly Lys His Gly Arg Lys Ile Lys Pro Pro Met 65 70 75 80 Lys Ile Phe Gln Gly Lys Phe Ala Arg Ala Trp Phe Glu Glu Arg Ala 85 90 95 Lys Leu Thr Lys Pro Leu Asp Gly Ala Arg Glu Val Leu Glu Arg Leu 100 105 110 Lys Glu Asp Gly His Ile Val Ile Ser Phe Ser Ala Glu Asp Phe Ile 115 120 125 Pro Gly Ile Lys Glu Tyr Arg Leu Lys Ile Ser Gly Leu Tyr Asp Leu 130 135 140 Phe Asp Asp Val Ile Ile Phe Gly Arg Asp Ile Thr Ile Cys Glu Ala 145 150 155 160 Phe Ser Ile Val Arg Glu Lys Tyr Gly Tyr Asp Thr Phe Val Trp Val 165 170 175 Asp Asp Lys Pro Trp Arg Phe Ile Gly Arg Gly Asp Glu Asn Thr Glu 180 185 190 Tyr Val Trp Met Tyr Phe Pro Tyr Thr Ala Arg Phe Val Ser Asp Asp 195 200 205 Ile Leu Ala Gln Ile Pro His Leu His Val Ile Tyr Asp Leu Trp Ser 210 215 220 Leu Leu Asp Val Val Lys Asn Leu Lys Ser Arg 225 230 235 <210> 141 <211> 211 <212> PRT <213> Pyrococcus furiosus <400> 141 Met Leu Ile Ile Leu Asp Leu Asp Asp Thr Leu Cys Asn Thr Trp Glu 1 5 10 15 Ala Ile Arg Ile Ala Ala Ile Arg Leu Ile Pro Thr Leu Leu Arg Leu 20 25 30 Arg Lys Phe Arg Met Phe Ala Tyr Ile Leu Thr Lys Arg Tyr Arg Glu 35 40 45 Leu Glu Glu Ile Arg Glu Leu His Ile Leu Gly Phe Glu Glu Ile Leu 50 55 60 Asp Arg Ile Ile Asn Lys Ile Tyr Lys Asn Leu Glu Asn Glu Glu Val 65 70 75 80 Gln Glu Ile Ala Asn Leu Phe Asp Arg Thr Phe Phe Ala Asn Leu Arg 85 90 95 Leu Tyr Pro Asp Val Ile Pro Phe Leu Glu Lys Val Arg Glu Met Gly 100 105 110 Ala Lys Ile Val Leu Val Thr Asp Ser Ser Ser Ser Trp Gln Arg Arg 115 120 125 Lys Leu Glu Val Leu Gly Ile Lys Asp Tyr Phe Asp Ala Ile Ile Val 130 135 140 Ser Gly Asp Thr Gly His Ser Lys Phe Glu Pro Tyr Asn Phe His Leu 145 150 155 160 Ala Arg Lys Lys Phe Pro Arg Glu Lys Lys Val Tyr Val Ile Gly Asp 165 170 175 Arg Asp Asp Thr Asp Met Lys Gly Gly Lys Ala Ile His Ala Thr Thr 180 185 190 Ile Leu Val Lys Arg Ala Thr Leu Lys Glu Gly Gly Gln Ser Met Gln 195 200 205 Ile Met Leu 210 <210> 142 <211> 238 <212> PRT <213> Pyrococcus furiosus <400> 142 Met Trp Ile Val Phe Asp Val Asp Gly Val Leu Ile Asp Thr Ser Glu 1 5 10 15 Ser Tyr Asp Met Ala Thr Lys Leu Thr Val Glu Tyr Phe Leu Ser His 20 25 30 Leu Gly Ile Lys Lys Glu Val Ser Leu Glu Ile Ile Arg Asp Met Arg 35 40 45 Lys Lys Gly Val Phe Ser Asp Asp Phe Glu Leu Ser Glu Ala Leu Ile 50 55 60 Leu Met Tyr Leu Ser Gly Glu Asp Leu Pro Lys Gly Glu Gly Val Glu 65 70 75 80 Tyr Leu Arg Gln Lys Leu Gly Ile Val Leu Asp Arg Lys Asp Ile Glu 85 90 95 Arg Val Phe Asn Thr Phe Tyr Leu Gly Glu Ile Tyr Pro Asn Ser Ile 100 105 110 Phe Lys Phe Glu Gly Leu Trp Arg Lys Glu Lys Arg Ile Val Asp Ile 115 120 125 Glu Leu Leu Lys Lys Ala Lys Glu Thr Tyr Lys Leu Gly Ile Val Thr 130 135 140 Gly Arg Asp Ser Leu Glu Met Lys Leu Ala Glu Glu Ile Ile Arg Phe 145 150 155 160 Lys Phe Asp Lys Val Val Thr Arg Asp Met Phe Glu Lys Pro Asp Pro 165 170 175 Arg Ala Leu Tyr Glu Val Thr Gln Gly Glu Glu Ala Ile Tyr Ile Gly 180 185 190 Asp Ser Lys Val Asp Glu Glu Leu Val Glu Arg Tyr Arg Arg Thr Phe 195 200 205 Lys Gly Asn Val Thr Tyr Leu Met Val Gly Arg Asp Val Asn Asp Val 210 215 220 Asn Glu Ala Leu Lys Lys Ile Leu Phe Gln Ser Tyr Ile Arg 225 230 235 <210> 143 <211> 219 <212> PRT <213> Pyrococcus furiosus <400> 143 Met Val Arg Val Ile Phe Phe Asp Ile Asp Gly Thr Leu Leu Thr Glu 1 5 10 15 Trp Pro Leu Val Lys Leu Met Leu Pro Gln Val Tyr Glu Met Leu Ala 20 25 30 Lys Lys Leu Gly Val Ser Lys Lys Glu Ala Arg Glu Ile Phe Leu Gly 35 40 45 Glu Ile Glu Lys Arg Lys Gly Thr Tyr Glu Trp Tyr Asp Trp Asn Phe 50 55 60 Phe Phe Ser Tyr Phe Asn Leu Pro Leu Arg Tyr Glu Asp Phe Ile Arg 65 70 75 80 Lys Tyr Pro Glu Lys Ile Glu Leu Tyr Pro Gly Val Arg Glu Val Leu 85 90 95 Lys Glu Leu Ser Gly Lys Tyr Lys Leu Gly Ile Ile Thr Ser Gly Pro 100 105 110 His Tyr Gln Leu Leu Lys Leu Lys Val Thr Asp Ile Asp Lys Phe Phe 115 120 125 Asp Val Ile Ile Thr Arg Asp Asp Val Lys Ala Val Lys Pro Ser Pro 130 135 140 Lys Ile Phe Leu Ala Gly Leu Glu Arg Val Arg Ala Lys Pro Thr Glu 145 150 155 160 Ser Leu Met Val Gly Asp Ser Leu Glu Asn Asp Ile Leu Gly Ala Lys 165 170 175 Ala Leu Gly Phe Lys Thr Val Trp Ile Asn Arg Gly Arg Glu Lys Gly 180 185 190 Phe Asn Leu Pro Asp Phe Glu Ile Tyr Glu Met Lys Glu Leu Ile Arg 195 200 205 Val Val Glu Val Ala Glu Asn Glu Lys Asp Ile 210 215 <210> 144 <211> 230 <212> PRT <213> Pyrococcus furiosus <400> 144 Met Ser Phe Leu Ala Ile Tyr Lys Thr Asp Val Lys Leu Leu Arg Arg 1 5 10 15 Asp Pro Met Leu Leu Tyr Ser Val Ile Met Val Phe Val Leu Leu Leu 20 25 30 Ile Val Arg Tyr Phe Lys Asp Lys Val Gly Ile Tyr Tyr Tyr Pro Leu 35 40 45 Ala Leu Leu Thr Met Leu Phe Ile Pro Met Ile Phe Gly Met Leu Pro 50 55 60 Gly Phe Val Met Ala Asp Glu Lys Glu Glu Lys Thr Ile Gln Ala Leu 65 70 75 80 Gln Val Ile Pro Ile Ser Ser Glu Ala Phe Leu Ala Tyr Arg Leu Thr 85 90 95 Trp Ala Ser Ile Val Thr Ala Val Leu Gly Phe Ala Ser Pro Tyr Ile 100 105 110 Leu Asp Ile Glu Leu Ser Arg Lys Gly Leu Ala Met Leu Ile Val Leu 115 120 125 Phe Val Leu Glu Val Trp Ile Tyr Gly Leu Leu Ile Thr Val Phe Ser 130 135 140 Glu Ser Arg Met Gln Ala Ile Thr Val Ser Lys Val Leu Gly Trp Phe 145 150 155 160 Leu Met Leu Pro Pro Leu Ile Lys Leu Val Val Leu Trp Arg Asn Leu 165 170 175 Ser Arg Asp Trp Ser Glu Phe Thr Ala Phe Leu Pro Thr Tyr Trp Leu 180 185 190 Tyr Lys Val Phe Glu Gly Ile Leu Gln Asn Asp Tyr Ser Asp Phe Leu 195 200 205 Val Ala Phe Ile Val His Leu Ile Trp Leu Ile Pro Leu Ile Ala Leu 210 215 220 Phe Lys Arg Arg Val Leu 225 230 <210> 145 <211> 240 <212> PRT <213> Pyrococcus furiosus <400> 145 Met Arg Arg Ile Lys Val Ile Phe Phe Asp Leu Asp Asp Thr Leu Val 1 5 10 15 Asp Thr Ser Lys Leu Ala Glu Val Ala Arg Lys Asn Ala Ile Glu Asn 20 25 30 Met Ile Arg His Gly Met Pro Val Asp Phe Asp Thr Ala Tyr Asn Glu 35 40 45 Leu Leu Glu Leu Ile Lys Glu Tyr Gly Ser Asn Phe Pro Tyr His Phe 50 55 60 Asp Tyr Leu Leu Arg Arg Leu Asp Leu Glu Tyr Asn Pro Lys Trp Val 65 70 75 80 Ala Ala Gly Val Ile Ala Tyr His Asn Thr Lys Phe Thr Tyr Leu Arg 85 90 95 Glu Val Pro Gly Ala Arg Lys Thr Leu Leu Arg Leu Lys Lys Glu Gly 100 105 110 Tyr Met Thr Gly Ile Ile Thr Asp Gly Asn Pro Ile Lys Gln Trp Glu 115 120 125 Lys Ile Leu Arg Leu Glu Leu Asp Asp Phe Phe Glu His Val Met Ile 130 135 140 Ser Asp Phe Glu Gly Val Lys Lys Pro His Pro Lys Ile Phe Lys Lys 145 150 155 160 Ala Leu Lys Ala Phe Asn Val Lys Pro Glu Glu Ala Ile Met Val Gly 165 170 175 Asp Arg Leu Tyr Ser Asp Ile Tyr Gly Ala Lys Asn Val Gly Met Lys 180 185 190 Thr Val Trp Phe Lys Tyr Gly Lys Tyr Ala Glu Leu Asp Leu Glu Tyr 195 200 205 Lys Glu Tyr Ala Asp Tyr Val Ile Thr Glu Leu Pro Gln Leu Leu Glu 210 215 220 Val Leu Glu Arg Glu Asn Gly Ser Asp Lys Glu Val His Ser Ser Gly 225 230 235 240 <210> 146 <211> 218 <212> PRT <213> Pyrococcus furiosus <400> 146 Met Ile Lys Gly Val Ile Phe Asp Val Asp Glu Thr Leu Val Tyr Tyr 1 5 10 15 Glu Gly Tyr Ser Leu Lys Glu Trp Tyr Glu Lys Ile Ala Lys Pro Thr 20 25 30 Met Lys Lys Leu Gly Ile Leu Ile Asp Trp Glu Thr Phe Arg Lys Met 35 40 45 Ala Lys Gly Glu Leu Pro Arg Ser Tyr Val Glu Glu Phe Gly Ile Asp 50 55 60 His Val Thr Phe Trp Lys Ala Leu Asp Lys Ala Asn Arg Arg Tyr Arg 65 70 75 80 Glu Glu Leu Leu Asn Glu Gly Lys Ile Arg Thr Tyr Asp Asp Val Glu 85 90 95 Ala Ile Lys Glu Leu Lys Lys Leu Gly Leu Lys Leu Ala Ala Val Ser 100 105 110 Asn Ala Ser Gln Asp Asn Thr Glu Leu Val Leu Lys Ala Phe Asn Leu 115 120 125 Leu Asp Tyr Phe Asp Val Val Tyr Gly Lys Asp Tyr Thr Tyr Leu Asp 130 135 140 Gly Val Lys Pro Asn Pro Tyr Leu Ile Asn Lys Ala Leu Asn Thr Leu 145 150 155 160 Gly Leu Arg Pro Asp Glu Ala Ile Leu Val Gly Asp Ser Asp Leu Asp 165 170 175 Ile Ile Ala Gly Lys Arg Ala Gly Val Thr Val Val Gln Ile Val Arg 180 185 190 Glu Lys Lys Tyr Glu Gly Ala Asp Tyr Tyr Ile Gln Asn Leu Trp Glu 195 200 205 Leu Val Asp Leu Ile Lys Arg Ser Arg Cys 210 215 <210> 147 <211> 234 <212> PRT <213> Pyrococcus furiosus <400> 147 Met Ile Lys Ala Val Phe Phe Asp Phe Ile Gly Thr Leu Leu Ser Gln 1 5 10 15 Glu Gly Glu Tyr Glu Thr His Leu Lys Ile Met Glu Glu Val Leu Gly 20 25 30 Glu Asn Lys Lys Ile Ser Pro Glu Glu Leu Leu Arg Glu Tyr Asp Ala 35 40 45 Leu Thr Arg Glu Ala Phe Ser Gln Tyr Ala Gly Lys Pro Phe Lys Pro 50 55 60 Ile Arg Ile Ile Glu Glu Glu Ile Met Lys Lys Leu Ala Glu Lys Tyr 65 70 75 80 Gly Phe Asp Tyr Pro Glu Asn Phe Trp Glu Ile His Leu Lys Ala His 85 90 95 Gln Arg Tyr Gly Lys Leu Tyr Pro Glu Val Val Glu Val Leu Asn Glu 100 105 110 Leu Lys Lys Arg Glu Tyr His Val Gly Leu Ile Thr Asp Ser Asp Thr 115 120 125 Ala Tyr Leu Arg Ala His Leu Glu Ala Leu Gly Ile Ala Glu Leu Phe 130 135 140 Asp Ser Ile Thr Thr Ser Glu Glu Ala Gly Phe Phe Lys Pro His Pro 145 150 155 160 Arg Ile Phe Glu Val Ala Leu Lys Lys Ala Gly Val Lys Gly Ser Glu 165 170 175 Ala Val Tyr Val Gly Asp Asn Pro Ile Lys Asp Cys Gly Gly Ala Arg 180 185 190 Gln Leu Asp Met Leu Ser Ile Leu Val Asp Arg Lys Gly Glu Lys Lys 195 200 205 Glu Leu Trp Lys Glu Cys Glu Phe Val Ile Ser Asp Leu Arg Glu Val 210 215 220 Ile Gln Ile Val Glu Glu Leu Asn Gly Gln 225 230 <210> 148 <211> 254 <212> PRT <213> Pyrococcus furiosus <400> 148 Met Lys Leu Lys Phe Trp Arg Glu Val Ala Ile Asp Ile Ile Ser Asp 1 5 10 15 Phe Glu Thr Thr Ile Met Pro Phe Phe Gly Asn Pro Asp Gly Gly Lys 20 25 30 Leu Val Lys Ile Ser Pro Ser Gly Asp Glu Thr Lys Leu Val Asp Lys 35 40 45 Leu Ala Glu Asp Leu Ile Leu Ser Arg Ile Thr Glu Leu Gly Val Asn 50 55 60 Val Val Ser Glu Glu Val Gly Val Ile Asp Asn Glu Ser Glu Tyr Thr 65 70 75 80 Val Ile Val Asp Pro Leu Asp Gly Ser Tyr Asn Phe Ile Ala Gly Ile 85 90 95 Pro Phe Phe Ala Leu Ser Leu Ala Val Phe Lys Lys Asp Lys Pro Ile 100 105 110 Tyr Ala Ile Ile Tyr Glu Pro Met Thr Glu Arg Phe Phe Glu Gly Ile 115 120 125 Pro Gly Glu Gly Ala Phe Leu Asn Gly Lys Arg Ile Lys Val Arg Lys 130 135 140 Thr Pro Asp Glu Lys Pro Ser Ile Ser Phe Tyr Ser Arg Gly Lys Gly 145 150 155 160 His Glu Ile Val Lys His Val Lys Arg Thr Arg Thr Leu Gly Ala Ile 165 170 175 Ala Leu Glu Leu Ala Tyr Leu Ala Met Gly Ala Leu Asp Gly Val Val 180 185 190 Asp Val Arg Lys Tyr Val Arg Pro Thr Asp Ile Ala Ala Gly Thr Ile 195 200 205 Ile Ala Lys Glu Ala Gly Ala Leu Ile Lys Asp Ser Ala Gly Lys Asp 210 215 220 Ile Asp Ile Ser Phe Asn Ala Thr Asp Arg Leu Asp Val Ile Ala Val 225 230 235 240 Asn Ser Glu Glu Leu Leu Lys Thr Ile Leu Ser Leu Leu Glu 245 250 <210> 149 <211> 264 <212> PRT <213> Pyrodictium occultum <400> 149 Met Arg Gly Arg Gly Arg Gly Arg Leu Leu Gly Ala Val Leu Ala Gly 1 5 10 15 Val Ala Ser Ala Gly Glu Leu Ala Arg Leu Leu Pro Gly Arg Val Cys 20 25 30 Gly Val Ala Val Asp Ile Asp Gly Thr Ile Thr Glu Arg Arg Val Ser 35 40 45 Gly Asp Phe Arg Leu Ser Leu Glu Ala Val Glu Ala Leu Arg Arg Leu 50 55 60 Glu Asp Ala Gly Val Arg Val Ile Leu Val Thr Gly Asn Ser Val Met 65 70 75 80 Val Ala Ala Gly Val Ala Arg Tyr Ile Gly Val Arg Gly Pro His Val 85 90 95 Ala Glu Asn Gly Cys Leu Val Tyr Gln Arg Gly Ser Val Val His Ala 100 105 110 Cys Arg Gly Thr Ala Arg Ala Ala Ala Glu Ala Leu Glu Glu Glu Leu 115 120 125 Gly Gly Leu Leu Glu Pro Ser Trp Gln Asn Arg Cys Arg Ile His Asp 130 135 140 Tyr Ala Phe Leu Val Arg Arg Val Gly Pro Glu Glu Ala Trp Arg Met 145 150 155 160 Ala Glu Arg Val Leu Arg Glu Arg Gly Leu Arg Ala Lys Leu Ser His 165 170 175 Ser Gly Tyr Ala Leu His Val Arg Pro Leu Glu Ala Ser Lys Gly Leu 180 185 190 Gly Leu Arg Val Ala Met Arg Met Ala Gly Leu Glu Pro Gly Cys Val 195 200 205 Val Ala Val Gly Asp Ser Ala Met Asp Leu Glu Met Arg Asp Ala Gly 210 215 220 Val Thr Leu Ala Ala Val Gly Asn Ala Asp Pro Arg Leu Arg Glu Gly 225 230 235 240 Ala Asp Leu Ile Leu Pro Gly Glu Ser Gly Gly Gly Val Ala Leu Leu 245 250 255 Ala Arg Ala Ile Leu Glu Ser Leu 260 <210> 150 <211> 235 <212> PRT <213> Rhodothermus marinus <400> 150 Met His Leu Leu Leu Phe Asp Ile Asp Gly Thr Leu Ile Arg Thr Arg 1 5 10 15 Gly Phe Gly Arg Gln Thr Met Glu Ala Ala Leu Ser Glu Trp Leu Gly 20 25 30 Arg Pro Val Thr Thr Glu Gly Val Asp Phe Ala Gly Arg Thr Asp Pro 35 40 45 Ala Ile Leu Leu Asp Ile Leu Lys Ala Ser Gly Leu Pro Glu His Thr 50 55 60 Ala Arg His Leu Leu Pro Glu Ala Leu Glu Val Tyr Ser Arg Ala Met 65 70 75 80 Ile Arg Arg Leu Arg Pro Glu His Leu Glu Val Leu Pro Gly Val Val 85 90 95 Met Leu Leu Glu Glu Leu Ser Glu Trp Pro Asp Val Tyr Leu Gly Leu 100 105 110 Val Thr Gly Asn Leu Arg Pro Val Ala Phe His Lys Leu Ala Met Ala 115 120 125 Gly Leu Ala Gly Tyr Phe Gly Glu Gly Ala Phe Gly Cys Asp His Ala 130 135 140 Asn Arg Asn Glu Leu Pro Pro Leu Ala Ile Glu Arg Ile Arg Glu Ala 145 150 155 160 Thr Gly Tyr Pro Phe Thr Gly Ala Asp Ala Val Ile Ile Gly Asp Thr 165 170 175 Pro His Asp Val Ala Cys Ala Arg His Ala Gly Ala Ser Val Ala Val 180 185 190 Val Cys Thr Gly Gly Tyr Ser Arg Asp Ala Leu Glu Ala Cys Arg Pro 195 200 205 Asp Leu Leu Leu Glu Asp Leu Ser Asp Pro Glu Pro Leu Phe Lys Leu 210 215 220 Leu Thr Gln Gln Ala Leu Ser Arg Lys Ala Ser 225 230 235 <210> 151 <211> 279 <212> PRT <213> Rhodothermus marinus <400> 151 Met Phe Asp Leu Arg Arg Ala Asp Leu His Leu His Thr Ser Arg Ser 1 5 10 15 Asp Gly Arg Leu Ser Pro Ala Glu Leu Val Arg Arg Ala Arg Glu Ala 20 25 30 Gly Leu Tyr Cys Val Ala Ile Thr Asp His Asp Thr Ile Asp Gly Leu 35 40 45 Glu Glu Ala Arg Gln Ala Ala Ala Arg Trp Ala Met Val Val Ile Pro 50 55 60 Gly Val Glu Leu Ser Val Gln Val Glu Glu Glu Glu Val His Leu Leu 65 70 75 80 Gly Tyr Phe Phe Asp Pro Asp His Pro Ala Leu Arg Glu Ala Leu Thr 85 90 95 Ala Tyr Arg Lys Ala Arg Glu Glu Arg Leu Ala Ala Met Leu Ala Arg 100 105 110 Leu Gln Glu Val Gly Val Arg Leu Ser Glu Glu Gln Val Gln Thr Ala 115 120 125 Val Gly His Gly Val Pro Gly Arg Pro His Val Ala Arg Ala Leu Val 130 135 140 Ala Ala Gly Tyr Ala Glu Ser Tyr Arg Glu Ala Phe Gln Arg Tyr Leu 145 150 155 160 Leu Pro Gly Gly Pro Gly Tyr Val Pro Lys Pro Ala Trp Thr Ala Glu 165 170 175 Glu Ala Val Ala Val Leu His Glu Ala Gly Gly Ile Ala Val Leu Ala 180 185 190 His Pro Gly Glu His Leu Arg Asp Arg Val Phe Arg Ala Leu Leu Gln 195 200 205 Ala Gly Ile Asp Gly Ile Glu Val Ile His Pro Ala His Ser Tyr Tyr 210 215 220 Leu Val Gln His Tyr Arg Gln Val Ala Arg Asp Phe Gly Leu Leu Glu 225 230 235 240 Thr Gly Gly Ser Asp Tyr His Gly His Arg Pro Glu Asp Asp Ala Leu 245 250 255 Leu Gly Ala Cys Thr Ile Pro Tyr Pro Arg Val Glu Arg Leu Arg Ala 260 265 270 Thr Leu Gln Ala Thr Arg Ala 275 <210> 152 <211> 846 <212> PRT <213> Rhodothermus marinus <400> 152 Met Ser Ser Arg Arg Phe Ala Thr Ser Asp Leu Val Leu Ala Leu Ala 1 5 10 15 Gly Val Ile Gly Leu Val Leu Gly Leu Trp Leu Leu Pro Arg Gln His 20 25 30 Pro Asp Ala Ala Leu Gln His Met Leu Ser Ala Glu Ala Ala Arg His 35 40 45 Arg Ala Ala Ala Phe Leu Ala Gln Arg Gly Tyr Arg Val Asp Thr Thr 50 55 60 His Ala Leu Val Val Leu Arg Arg Ala Pro Glu Leu Leu Arg Arg Trp 65 70 75 80 Gln Thr Arg Trp Gly Arg Pro Glu Leu Val Arg Arg Leu Glu Asp Leu 85 90 95 Pro Trp Leu Pro Val Tyr Arg Trp Val Val Tyr Arg Ser Ser Asp Glu 100 105 110 Met Asn Gly Gln Arg Trp Gln Val Val Leu Ala Gly Asp Gly Thr Ile 115 120 125 Trp Gly Phe Arg Gly Pro Glu Thr Pro Gly Glu Asp Pro Ala Ala Arg 130 135 140 Ala Ala Val Gly Ile Ala Ala Glu Ala Phe Asp Pro Leu Ala Ser Trp 145 150 155 160 Gly Lys Leu Ser Gly Ala Pro Asp Ser Ser Glu Thr Pro Glu Arg Pro 165 170 175 Gly Pro Ala Ala Ala Val Ala Leu Ala Arg Tyr His Leu Gln Arg Thr 180 185 190 Ile Gly Ser Val Leu Pro Leu Arg Pro Asp Ser Val Gly Leu Leu Ala 195 200 205 Thr Ser Ser Pro Gln Gln Ala Val Val Arg Phe Arg Gly Leu Ser Pro 210 215 220 Thr Gly Asp Ser Val Thr Val Gln Val Val Val Thr Ala Ser Gly Gln 225 230 235 240 Leu Arg Ala Leu Glu Ala Thr Trp Gly Thr Leu His Leu Pro Glu Pro 245 250 255 Ser Pro Leu Glu Glu Gly Pro Pro Arg Arg Gly Glu Arg Gly Phe Thr 260 265 270 Val His Glu Val Gln Asp Leu Leu Ser Val Leu Leu Phe Ala Gly Leu 275 280 285 Gly Ile Trp Leu Leu Thr Val Phe Leu Arg Arg Leu His Arg Arg Leu 290 295 300 Leu Asp Thr Gln Gly Pro Leu Arg Asp Ala Val Leu Gly Gly Leu Ala 305 310 315 320 Phe Thr Val Ala Thr Leu Gly Gly Ala Leu Pro Gly Ile Leu Gln Val 325 330 335 Pro Asp Leu Trp Leu Arg Leu Leu Ile Leu Leu Met Ser Thr Leu Val 340 345 350 Val Gly Val Phe Gly Ala Ala Ser Val Phe Leu Leu Ala Gly Thr Ser 355 360 365 Asp Ala Leu Ala Arg Asp Arg Trp Pro Glu Lys Leu Ala Val Leu Thr 370 375 380 Leu Leu Arg Asn Gly Gln Val Arg Asn Val Val Val Gly Ala Ser Leu 385 390 395 400 Leu Arg Gly Leu Ala Leu Gly Gly Leu Leu Leu Gly Met Thr Val Gly 405 410 415 Leu Leu Gly Leu Trp Pro Arg Ala Ala Leu Arg Leu Asp Ala Gly Ala 420 425 430 Trp Thr Val Pro Trp Pro Gly Met Gln Ala Ala Val Trp Leu Gly Phe 435 440 445 Ala Val Trp Val Gly Met Leu Leu Val Tyr Leu Leu Leu Ala Val Met 450 455 460 Ala Arg Leu Pro Trp Arg Asp Gly Gly Arg Val Val Gly Thr Leu Thr 465 470 475 480 Leu Leu Leu Leu Leu Ala Gly Val Ser Ala Ile Asp Leu Glu Gln Val 485 490 495 Gly Phe Glu Leu Leu Val His Ala Leu Trp Gly Val Val Leu Ala Trp 500 505 510 Ala Cys Trp Arg Tyr Glu Pro Ala Cys Ser Gly Ile Gly Ala Met Thr 515 520 525 Ala Trp Ala Leu Trp Arg Ser Ala Ser Gly Trp Met Ala Pro Asp Gly 530 535 540 Pro Phe Gly Pro Asp Gly Trp Ile Val Leu Ala Met Val Gly Thr Gly 545 550 555 560 Leu Val Ile Gly Phe Val Gly Ile Arg Ser Arg Arg Ser Ala Ala Glu 565 570 575 Leu Pro Arg Tyr Val Pro Ala Tyr Leu Gln Glu Leu Ala Arg Gln Glu 580 585 590 Arg Leu Glu Arg Glu Leu Glu Ile Ala Arg Gln Ala Gln Ala Ser Leu 595 600 605 Leu Pro Arg Thr Leu Pro Glu Val Pro Gly Ala Ala Met Ala Ala Leu 610 615 620 Cys Arg Pro Ala Tyr Glu Val Gly Gly Asp Tyr Tyr Asp Val Phe Ala 625 630 635 640 Leu Pro Asp Gly Arg Leu Ala Val Val Val Gly Asp Val Ser Gly Lys 645 650 655 Gly Ile Gln Ala Ala Phe Phe Met Thr Leu Ile Lys Gly His Val Arg 660 665 670 Ala Leu Ser Leu Ser Thr Arg Asp Pro Ala Asp Val Leu Arg His Leu 675 680 685 Asn Arg Leu Phe Arg Glu Gln Ala Pro Arg Gly Leu Phe Val Thr Met 690 695 700 Ile Tyr Gly Val Leu Asp Pro Thr Thr Arg Thr Phe Thr Leu Ala Arg 705 710 715 720 Ala Gly His Pro Pro Val Leu His Tyr Arg Ala Cys Thr Gln Gln Val 725 730 735 Gln Cys Leu Arg Pro Ser Gly Met Gly Ile Gly Leu Ala Asp Ala Glu 740 745 750 Leu Phe Asp Lys Ala Leu Glu Asn Cys Val Leu Arg Leu Glu Ala Gly 755 760 765 Asp Arg Val Leu Leu Tyr Thr Asp Gly Ile Thr Glu Met Ala Gly Pro 770 775 780 Arg Gly Glu Arg Trp Gly Leu Glu Arg Leu Gln Gln Trp Leu Arg Glu 785 790 795 800 Ser Ser Arg Arg Gly Tyr Ala Pro Glu Gln Ala Leu Gly Ala Leu Glu 805 810 815 Glu Gln Leu Arg Ala Phe Ala Gly Thr Thr Glu Leu Ala Asp Asp Leu 820 825 830 Thr Ala Ile Leu Leu Glu Ala Lys Gln Asp Gly Tyr Ala Asn 835 840 845 <210> 153 <211> 534 <212> PRT <213> Rhodothermus marinus <400> 153 Met Pro Ala Val Val Ala Glu Leu Glu Gln Ala Thr Arg Ser Pro Val 1 5 10 15 Arg Met Cys Val Ile Asp Leu Gly Thr Asn Ser Phe His Ala Val Ile 20 25 30 Val Asp Ala Leu Pro Gly Thr Phe Arg Val Val Asp Arg Phe Lys Glu 35 40 45 Met Val Arg Leu Gly Glu Glu Gly Leu Gln Gly Gln Arg Leu Ser Glu 50 55 60 Ala Ala Met Gln Arg Ala Ile Arg Ala Leu Arg Arg Ile Arg Gln Leu 65 70 75 80 Ala Glu Gly Trp Gly Ala Thr Glu Phe Leu Ala Cys Ala Thr Ser Ala 85 90 95 Ile Arg Glu Ala Glu Asn Gly Gly Glu Leu Leu Gln Arg Ile Arg Ser 100 105 110 Glu Val Gly Leu His Val Arg Val Ile Asp Gly Leu Gln Glu Ala Arg 115 120 125 Leu Ile Tyr Lys Gly Val Arg Arg Ala Val Pro Met Pro Glu Pro Val 130 135 140 Leu Ile Met Asp Val Gly Gly Gly Ser Val Glu Phe Ile Val Gly Thr 145 150 155 160 Ser Gln Gln Pro Leu His Leu Phe Ser Leu Lys Leu Gly Ala Ala Arg 165 170 175 Met Thr Arg Arg Phe Val Arg His Asp Pro Ala Thr Arg Glu Glu Leu 180 185 190 Lys Ala Leu Arg Ala Phe Tyr Arg Glu Gln Leu His Pro Val Phe Glu 195 200 205 Ala Ala Arg Ala His Gly Val Arg Glu Val Val Gly Ser Ser Gly Ala 210 215 220 Met Glu Asn Leu Ala Ser Val Cys Ala Arg Met Arg Gly Glu Val Ala 225 230 235 240 Arg Ser Ile Tyr Glu Gln Pro Phe Pro Ala Glu Asp Phe Arg Arg Val 245 250 255 Ala Arg Arg Ile Met Arg Leu Ser Arg Glu Gln Arg Arg Arg Leu Arg 260 265 270 Gly Ile Asp Pro Lys Arg Val Asp Gln Ile Val Ala Ala Ala Ala Leu 275 280 285 Ile Asp Val Val Leu Lys Asp Leu Ala Ile Glu Arg Val Arg Ile Ser 290 295 300 Pro His Ala Leu Arg Glu Gly Leu Val Val Asp Phe Val Glu Arg Asn 305 310 315 320 Ala Pro Leu Leu Ala Arg Leu Thr Ala Phe Ala Asp Val Arg Arg Arg 325 330 335 Ser Ile Tyr Glu Met Gly Trp Arg Phe Asp Trp Glu Tyr Arg His Ala 340 345 350 Gln Gln Val Ala Ala Leu Ala Leu Gln Leu Phe Asp Ala Thr Gln Ser 355 360 365 Leu His Gly Leu Gly His Val Asp Arg Glu Leu Leu Glu Tyr Ala Ala 370 375 380 Leu Leu His Asp Ile Gly Tyr His Ile Ser His Arg Ser His His Lys 385 390 395 400 His Gly Leu Tyr Leu Ile Lys His Ala Asp Leu Arg Gly Phe Thr Ser 405 410 415 Glu Glu Ile Ala Val Leu Ala Asn Val Val Arg Tyr His Arg Gly Ser 420 425 430 Leu Pro Lys Pro Thr His Ala Asp Tyr Met Ala Leu Ser Glu Glu Asn 435 440 445 Arg Val Arg Val Cys Lys Leu Ala Ala Leu Leu Arg Leu Ala Glu Gly 450 455 460 Leu Asp Arg Ser His Asn Gln Asn Val Arg Thr Leu His Val Arg Leu 465 470 475 480 Glu Ser Asp Arg Leu Val Leu His Leu Glu Thr Arg Gly Asp Pro Glu 485 490 495 Leu Glu Val Trp Gly Val Arg Arg Ser Ala Glu Leu Phe Glu Gln Thr 500 505 510 Phe Gly Arg Thr Val Glu Val Ser Ala Thr Ala Glu Pro Pro Leu Leu 515 520 525 Glu Glu Thr Ala Gly Ala 530 <210> 154 <211> 336 <212> PRT <213> Rhodothermus marinus <400> 154 Met Thr Thr Asn His Ile Gln Glu Leu Leu Ser Lys Leu Asn Glu Leu 1 5 10 15 Arg Ala Val Phe Ile Leu Gly Gln Arg Ala Ile Pro Phe Ile Glu Glu 20 25 30 Val Val Tyr Phe Leu Arg Glu Ile Ser Pro Leu Leu Ser Glu Val Asn 35 40 45 Asp Ser Leu Ala Glu Ser Thr Arg Lys Met Pro Arg Ala Ser Ser Gln 50 55 60 Leu Lys Ser Val Thr Gln Ala Thr Glu Met Ala Thr Thr Glu Ile Leu 65 70 75 80 Asp Leu Ile Asp Val Val Leu Asn Asp Leu Asp Thr Phe Arg Lys Ser 85 90 95 Trp Ala Ala Ala Pro Glu Ala Leu Gln Thr Leu Gln Ser Gln Glu Glu 100 105 110 Gln Leu Trp Gln Pro Leu Pro Glu Ser Trp Asn Glu Arg Leu Gln Thr 115 120 125 Leu Leu Glu Glu Arg Arg Gln His Tyr Ala Thr Leu Gln Ala Met Leu 130 135 140 Glu Ser Gln Gln Gln Leu Val Asp Ser Leu Arg Asp Arg Met Asn Arg 145 150 155 160 Ile Met Met Ala Leu Gln Val Gln Asp Ile Thr Ala Gln Gln Leu Ala 165 170 175 Ala Val Asn His Leu Ile Glu Ser Val Arg Tyr Arg Met Ala Gln Leu 180 185 190 Ile Arg Arg Leu Gly Ser Glu Val Leu Asp Asp Leu Glu Leu Pro Arg 195 200 205 Gln Leu Phe Ala Glu Gly Thr Phe Asp Pro His Ala Arg Tyr Asp Arg 210 215 220 Asp Gly Arg Arg Gln Gln Gln Val Asp Ala Leu Val Asp Ala Leu Gln 225 230 235 240 His Asn Ala Ser Leu Pro Glu Asp Gly Glu Ser Thr Gly Pro Ala Ser 245 250 255 Gln Asp Glu Ile Asp Ala Leu Phe Ser Gly Asn Ser Thr Pro Ala Ser 260 265 270 Gln Asp Glu Ile Asp Ser Leu Phe Ser Ser Gly Thr Pro Thr Ser Gln 275 280 285 Asp Glu Ile Asp Ala Leu Phe Gly Gly Gly Ser Thr Pro Ala Ser Gln 290 295 300 Asp Glu Ile Asp Ala Leu Phe Gly Gly Gly Ser Thr Pro Ala Ser Gln 305 310 315 320 Asp Glu Ile Asp Ala Leu Phe Asn Pro Lys Arg Asn Asp Ser Asn Ser 325 330 335 <210> 155 <211> 231 <212> PRT <213> Rhodothermus marinus <400> 155 Met Ser Leu Leu Phe Thr Glu Glu Glu Leu Thr Arg Val Arg Glu Ala 1 5 10 15 Val Ala Ala Ala Glu Gln Arg Thr Ala Gly Glu Ile Val Pro Val Ile 20 25 30 Val Pro Arg Ser Gly Ala Tyr Pro Glu Ala Val Trp Lys Gly Ala Val 35 40 45 Leu Leu Met Leu Pro Val Leu Ala Val Ala Leu Leu Phe Asp Tyr Ile 50 55 60 Tyr Gln Gly Trp Gly Leu Thr Leu Leu His Thr Gly Trp Gly Val Ala 65 70 75 80 Leu Leu Thr Ala Leu Ala Gly Leu Val Gly Gly Leu Leu Gly Ala Tyr 85 90 95 Val Ala Pro Val Gln Arg Trp Leu Val Gly Glu Ala Arg Met Ala Glu 100 105 110 Gln Val His Leu Arg Ala Leu Gln Ala Phe Leu Glu Glu Glu Val Phe 115 120 125 Asn Thr Arg Asp Arg Thr Gly Ile Leu Ile Phe Val Ser Leu Phe Glu 130 135 140 His Trp Val Glu Val Ile Gly Asp Ala Gly Ile Asn Gln Arg Val Gly 145 150 155 160 Pro Glu Ala Trp Ala Glu Val Val Asp Arg Ile Arg Lys Gly Ile Arg 165 170 175 Glu Gly Arg Pro Val Asp Gly Leu Val Ala Ala Ile Glu Gln Cys Gly 180 185 190 Gln Leu Leu Ala Thr His Gly Val Ala Leu Arg Pro Asp Asp Thr Asn 195 200 205 Glu Leu Ala Asp Thr Leu Arg Val Arg Gly Ala Pro Ser Lys Lys Arg 210 215 220 Arg Gly Gly Arg Arg Lys Arg 225 230 <210> 156 <211> 221 <212> PRT <213> Rhodothermus marinus <400> 156 Met Pro Ala Thr Thr Leu Tyr Phe Val Arg His Gly Glu Thr Asp Tyr 1 5 10 15 Asn Arg Asn Gly Ile Val Gln Gly Arg Gly Val Asp Ala Pro Leu Asn 20 25 30 Glu Arg Gly Arg Arg Gln Ala Glu Ala Leu Ala Arg Arg Phe Ala Ala 35 40 45 Val Pro Leu Asp Ala Ile Tyr Ala Ser Pro Leu Arg Arg Ala Leu Glu 50 55 60 Thr Ala Glu Ala Val Arg Arg Tyr His Pro Glu Val Pro Phe Tyr Gln 65 70 75 80 Leu Ala Asp Leu Glu Glu Met Asp Trp Gly Asp Leu Glu Gly Lys Pro 85 90 95 Tyr Ala Pro Pro Tyr Asp Ala Lys Ile Arg Ala Ile Tyr Glu Arg Trp 100 105 110 Arg Ala Gly Asp Tyr Asp Tyr Pro Val Pro Gly Gly Glu Ser Ile Leu 115 120 125 Asp Val Gln Arg Arg Ala Leu Arg Ala Leu Glu Thr Ile Leu Ser Arg 130 135 140 His Glu Gly Glu Thr Val Leu Ile Val Ala His Gly Arg Phe Leu Arg 145 150 155 160 Ile Leu Leu Ala Ser Val Leu Ser Glu Tyr Gly Leu Ala Arg Met Glu 165 170 175 Ala Leu Pro His Thr Asn Thr Ala Val Asn His Leu Val Tyr Glu Asn 180 185 190 Gly Arg Phe Arg Ala Leu Arg Leu Asn Cys Thr Ala His Leu Glu Glu 195 200 205 Ala Ala Val Asp Asp Gly Leu Pro Thr Glu Val Ala Ala 210 215 220 <210> 157 <211> 162 <212> PRT <213> Rhodothermus marinus <400> 157 Met Arg Leu Cys Leu Leu Arg His Ala Glu Ala Tyr Pro Ala Ala Pro 1 5 10 15 Gly Arg Pro Asp Ala Glu Arg Ser Leu Thr Glu Val Gly Gln Gln Val 20 25 30 Ala Arg Gln Met Gly Glu Ala Leu Arg Arg Leu Arg Leu Ala Pro Gly 35 40 45 Ala Val Tyr Thr Ser Pro Tyr Arg Arg Ala Val Gln Thr Ala Gln Ala 50 55 60 Val Ala Glu Ala Leu Gly Val Pro Val Val Glu Asp Arg Leu Leu Ala 65 70 75 80 Pro Gly Cys Gly Pro Ala Glu Leu Glu Thr Leu Ile Gln Ala Tyr Ala 85 90 95 Pro Gly Glu Thr Val Leu Val Val Gly His Gln Pro Asp Phe Gly Glu 100 105 110 Leu Val Arg Trp Leu Thr Gly Ala Thr Ile Arg Leu Pro Ala Gly Gly 115 120 125 Leu Ala Val Val Glu Thr Pro Ala Leu Arg Glu Arg Ala Gly Thr Leu 130 135 140 His Gly Leu Tyr Asp Pro Ala Trp Leu Ala Ala Val Met Thr Gly Arg 145 150 155 160 Thr Gly <210> 158 <211> 249 <212> PRT <213> Rhodothermus marinus <400> 158 Met Asn Val Glu Val Phe Leu Thr Gly Ser His Val Thr Glu Glu Asp 1 5 10 15 Val Glu Gly Arg Thr Val Val Val Ile Asp Val Leu Arg Ala Ser Ser 20 25 30 Thr Ile Ile Thr Ala Leu Ala Asn Gly Ala Arg Ala Val Ile Pro Val 35 40 45 Ala Asp Met Asp Gln Ala Gly Lys Ile Ala Met Asn Leu Asp Pro Ser 50 55 60 Thr Tyr Leu Leu Gly Gly Glu Arg Asp Gly Asp Arg Ile Asp Gly Tyr 65 70 75 80 His Leu Gly Asn Ser Pro Leu Glu Tyr Thr Pro Asp Val Val Glu Gly 85 90 95 Lys Thr Ile Ile Leu Asn Thr Thr Asn Gly Thr Arg Thr Ile Trp Asn 100 105 110 Ala Arg Asn Ala Glu His Leu Ile Val Gly Gly Phe Leu Asn Ala Asn 115 120 125 Arg Val Val Gln Phe Val Arg Glu Ala Gly Leu Asp Val Thr Ile Ile 130 135 140 Cys Ala Gly Arg Asn Asn Arg Val Ala Leu Asp Asp Ala Leu Cys Ala 145 150 155 160 Gly Leu Leu Leu His Arg Leu Trp Glu Gly Arg Glu Pro Glu Tyr Val 165 170 175 Ser Asp Ala Ala His Ile Ala Leu Thr Gln Tyr Leu His Asp Arg Asp 180 185 190 Arg Leu Ala Asp Ala Leu Arg His Ser Asn His Ala Arg Trp Leu Ile 195 200 205 Glu Lys Gly Tyr Gly Ala Asp Val Glu Tyr Cys Leu Gln Leu Asp Ala 210 215 220 Leu Pro Val Leu Pro Tyr Tyr Arg Glu Asn Arg Leu Val Leu Tyr Arg 225 230 235 240 Glu Arg Gln Pro Ser Glu Ser Val Gly 245 <210> 159 <211> 345 <212> PRT <213> Rhodothermus marinus <400> 159 Met Glu Thr Leu His Thr Arg Gly Arg Lys Val Val Glu Glu Phe Ile 1 5 10 15 Thr Leu Glu Gln Phe Ile Ile Asp Gln Gln Glu Arg Phe Pro His Ser 20 25 30 Thr Gly Ala Phe Ser Arg Leu Leu Arg Asp Ile Ser Val Ala Ala Lys 35 40 45 Ile Val Asn Arg Asp Ile Arg Arg Ala Gly Leu Val Asp Ile Phe Gly 50 55 60 Thr Thr Gly Lys Val Asn Ile His Gly Glu Val Gln Gln Lys Leu Asp 65 70 75 80 Ala Leu Ala His Glu Glu Phe Val Arg Ala Leu Arg Arg Gly Gly Glu 85 90 95 Cys Cys Leu Ile Gly Ser Glu Glu His Ala Glu Ala Ile Pro Leu Ser 100 105 110 Ala Asn Gly Glu Gly Asp Gly Arg Tyr Ile Val Leu Leu Asp Pro Leu 115 120 125 Asp Gly Ser Ser Asn Val Asp Val Asn Val Ser Val Gly Thr Ile Phe 130 135 140 Ser Ile Tyr Arg Leu Pro Asp Glu Tyr Glu Thr Pro Thr Leu Glu Ala 145 150 155 160 Ala Leu Gln Pro Gly Ser Lys Gln Val Ala Ala Gly Tyr Ile Val Tyr 165 170 175 Gly Ser Ser Thr Met Leu Val Tyr Thr Thr Gly Asn Gly Val Asn Gly 180 185 190 Phe Thr Leu Asp Pro Ser Ile Gly Glu Phe Ile Leu Ser His Pro Asn 195 200 205 Ile Arg Ile Pro Lys Thr Gly Ser Ile Tyr Ser Ile Asn Glu Gly Asn 210 215 220 Phe Asn Ser Phe Glu Glu Gly Leu Lys Arg Phe Ile Arg Trp Ala Gln 225 230 235 240 Glu Glu Asp Lys Ala Thr Gly Arg Pro Phe Ser Thr Arg Tyr Ile Gly 245 250 255 Ser Phe Val Ser Asp Phe His Arg Asn Leu Leu Lys Gly Gly Ile Tyr 260 265 270 Met Tyr Pro Ala Thr Lys Lys Asn Pro Glu Gly Lys Leu Arg Leu Met 275 280 285 Tyr Glu Ala Asn Pro Met Ala Phe Ile Val Glu Gln Ala Gly Gly Arg 290 295 300 Ala Ser Asp Gly His Arg Arg Ile Leu Asp Ile Val Pro Glu Lys Leu 305 310 315 320 His Gln Arg Thr Pro Leu Phe Ile Gly Ser Glu Glu Leu Val Arg Thr 325 330 335 Val Glu Glu Phe Leu Gln Gly Lys Arg 340 345 <210> 160 <211> 197 <212> PRT <213> Rhodothermus marinus <400> 160 Met Lys Arg Arg Gly Leu Trp Ser Gly Thr Val Leu Ala Val Leu Ile 1 5 10 15 Thr Ala Ser Ala Gln Ala Gln Thr Ala Cys Asp Arg Pro Ala Leu Glu 20 25 30 Leu Arg Leu Leu Cys Thr Val Tyr Arg Trp Asp Gly Pro Val Ala Ser 35 40 45 Ala Tyr Phe Glu Thr Ile Asp Ala Thr Ala Tyr Pro Met Phe Ala Gly 50 55 60 Leu Thr Ala Met Ala Trp Gly Gly Val Leu Ala Gly Arg Leu Glu Arg 65 70 75 80 Pro Ala Ala Glu Arg Val Thr Leu Ala Thr Ala Ala Thr Thr Val Leu 85 90 95 Val Phe Gly Leu Lys Ala Leu Ala Arg Arg Asn Arg Pro Phe Asp Ala 100 105 110 Trp Asp Asp Ile Ala Pro Arg Gly Asp Pro Pro Thr Ser His Ala Phe 115 120 125 Pro Ser Gly His Ala Ala Leu Ala Phe Thr Leu Ala Thr Ala Trp Gly 130 135 140 Leu Glu Val Pro Arg Ile Tyr Val Ile Val Pro Ala Tyr Val Trp Ala 145 150 155 160 Thr Ser Val Ala Val Gly Arg Val Trp Lys Gly Val His Tyr Pro Thr 165 170 175 Asp Val Leu Ala Gly Ala Val Leu Gly Ala Gly Val Ala Trp Thr Val 180 185 190 His Arg Ile Trp Arg 195 <210> 161 <211> 269 <212> PRT <213> Rhodothermus marinus <400> 161 Met Ser Trp Trp Glu Ala Leu Ile Leu Gly Leu Leu Gln Gly Leu Thr 1 5 10 15 Glu Phe Leu Pro Val Ser Ser Ser Gly His Leu Val Leu Gly Gln Tyr 20 25 30 Val Leu Gly Leu Asn Pro Gly Gly Val Thr Phe Glu Val Phe Val His 35 40 45 Phe Gly Thr Val Leu Ser Ile Leu Thr Val Tyr Arg Lys Arg Val Gly 50 55 60 Ala Ile Val Gly Glu Val Trp Thr Ala Leu Pro Arg Pro Ala Glu Trp 65 70 75 80 Pro Met Arg Tyr Arg Glu Arg Asp Pro Phe Arg Leu Ala Val Trp Ile 85 90 95 Leu Ile Thr Met Ile Pro Thr Gly Leu Val Tyr Val Leu Leu Gly Asp 100 105 110 Trp Ile Glu Gln Thr Phe Glu His Pro Arg Phe Ala Ala Gly Met Leu 115 120 125 Val Val Thr Gly Val Leu Leu Ile Leu Thr Arg Leu Arg Arg His Pro 130 135 140 Asp Gly Asp Leu Ser Pro Leu Lys Ala Phe Val Val Gly Val Ala Gln 145 150 155 160 Ser Ala Ala Met Leu Pro Gly Ile Ser Arg Ser Gly Ser Thr Ile Cys 165 170 175 Ala Ala Ile Tyr Gln Asn Val Arg Pro Glu Arg Ala Ala Asp Phe Ser 180 185 190 Phe Leu Met Leu Leu Pro Val Val Leu Gly Ala Thr Leu Leu Lys Gly 195 200 205 Ile Glu Leu Leu Asn Ser Pro Glu Ser Ile Gly Met Gly Pro Leu Val 210 215 220 Leu Gly Thr Val Ala Ala Tyr Val Ser Gly Val Ala Ala Ile Arg Met 225 230 235 240 Leu Leu Gln Val Val Arg Arg Gly Arg Leu Glu Tyr Phe Ala Tyr Tyr 245 250 255 Cys Phe Leu Val Gly Leu Leu Gly Leu Trp Leu Ile Arg 260 265 <210> 162 <211> 366 <212> PRT <213> Rhodothermus marinus <400> 162 Met Ser Thr Arg Arg Ala Val Leu Asn Cys Phe Arg Arg His Gln Arg 1 5 10 15 Phe Val Leu Thr Thr His Ile Lys Pro Asp Gly Asp Ala Leu Gly Ser 20 25 30 Gln Leu Ala Leu Gly Arg Leu Leu Gln Lys Met Gly His Asp Val Tyr 35 40 45 Leu Ile Asn Ser Asp Pro Pro Pro Ser Asn Leu Thr Trp Ile Pro Gly 50 55 60 Ile Glu Gln Val Glu Val Phe Asn Gly Ala Leu Ala Gln Arg Glu Arg 65 70 75 80 Ile Asp Gln Ala Asp Val Ile Cys Val Leu Asp Thr Asn Ala Leu Asp 85 90 95 Arg Leu Gly Asp Leu Ala Pro Ala Val Glu Ala Ser Arg Ala Arg Lys 100 105 110 Leu Leu Ile Asp His His Thr Ser Pro Glu Asp Trp Phe Asp Leu Gln 115 120 125 Tyr Val Arg Asp Thr Ala Ser Ser Thr Gly Glu Leu Val Tyr Glu Leu 130 135 140 Val Cys Ala Val Asp Pro Asn Leu Ile Asp His Glu Leu Ala Thr Ala 145 150 155 160 Leu Tyr Val Ala Ile Met Thr Asp Thr Gly Ser Phe Arg Phe Asn Thr 165 170 175 Val Thr Pro Thr Val His Arg Ile Val Ala Asp Leu Leu Glu Arg Gly 180 185 190 Gly Leu Ser Thr Glu Ala Ile His Ser Ala Ile Phe Asp Thr Arg Thr 195 200 205 Pro Glu Ser Met Arg Leu Leu Gly Leu Ala Leu Arg Asn Leu Gln Leu 210 215 220 Arg Tyr Asp Gly Arg Val Ala Tyr Met Val Leu Ser Arg Arg Met Phe 225 230 235 240 Asn Glu Thr Gly Ala Ser Thr Glu Asp Thr Glu Gly Phe Ile Asn His 245 250 255 Leu Leu Ser Ile Arg Gly Val Arg Val Ala Leu Leu Phe Thr Glu Ile 260 265 270 Glu Lys Gly Val Lys Ile Ser Phe Arg Ser Lys Gly Asp Tyr His Val 275 280 285 Asn Glu Trp Ala Arg Ala Phe Gly Gly Gly Gly His Arg Asn Ala Ala 290 295 300 Gly Ala Phe Val Glu Asn Ala Ala Leu Asp Ala Leu Val Asp Ala Val 305 310 315 320 Leu Ala Ala Ala Pro Arg Tyr Leu Pro Gln Leu Glu Ala Ser Ser Ala 325 330 335 Gly Asn Ala Ser Gly Thr Leu Ser Ser Glu Asp Ala Ser Tyr Leu Ser 340 345 350 Ala Leu Leu His Gln Lys Ser Gln Ala Ser Ser Thr Ala Ser 355 360 365 <210> 163 <211> 174 <212> PRT <213> Rhodothermus marinus <400> 163 Met Ser Gln Thr Asn His Gln Pro Ile Arg Val Leu Phe Val Cys Leu 1 5 10 15 Gly Asn Ile Cys Arg Ser Pro Leu Ala Glu Gly Val Phe Arg Lys Leu 20 25 30 Val Asp Glu Ala Gly Leu Thr Ala His Phe Glu Ile Asp Ser Ala Gly 35 40 45 Thr Gly Pro Trp His Val Gly Glu Pro Ala Asp Arg Arg Met Gln Arg 50 55 60 Thr Ala Arg Arg His Gly Val Asp Leu Ser Gly His Val Ala Arg Gln 65 70 75 80 Leu Gly Arg Glu Asp Leu Ala Arg Tyr Asp His Ile Tyr Val Met Asp 85 90 95 Arg Glu Asn Leu Glu Asp Val Leu Arg Leu Asp Arg Asp Gly Arg Phe 100 105 110 Arg His Lys Val Glu Leu Phe Arg Thr Phe Asp Pro Glu Pro Gly Asp 115 120 125 Gly Glu Val Pro Asp Pro Tyr Tyr Gly Gly Glu Arg Gly Phe Glu Glu 130 135 140 Val Tyr Gln Ile Val Glu Arg Thr Ala Arg Arg Leu Leu Glu His Leu 145 150 155 160 Val Ser Leu Tyr Lys Leu Lys Glu Thr Ala Asp Leu Ser Arg 165 170 <210> 164 <211> 272 <212> PRT <213> Rhodothermus marinus <400> 164 Met Met Asp Ala Tyr Thr Leu Tyr Glu Glu Ala Arg Glu Val Ala Ala 1 5 10 15 Arg Leu Ala Arg Asp Ala Gly Gln Ile Ala Arg Tyr Tyr Ala Gly Arg 20 25 30 Val Thr Val Arg Glu Lys Gly Tyr Asn Glu Leu Val Thr Gln Ala Asp 35 40 45 Glu Glu Val Gln Arg Phe Leu Ile Glu Gln Ile His Arg His Phe Pro 50 55 60 Glu His Ala Ile Leu Ala Glu Glu Asn Leu Ser Asp Met Gln Asp Gly 65 70 75 80 Arg Glu Gly Ala Ser Phe Arg Trp Ile Ile Asp Pro Ile Asp Gly Thr 85 90 95 Thr Asn Phe Thr His Gly Val Pro Pro Tyr Gly Ile Ser Leu Ala Leu 100 105 110 Gln His Glu Gly Arg Thr Val Val Gly Val Val Tyr Asp Val Pro His 115 120 125 Asp Glu Leu Phe Thr Ala Val Arg Gly Gly Gly Leu Tyr Val Asn Gly 130 135 140 Val Arg Ala Arg Val Ser Gln Thr Glu Thr Leu Arg Glu Ala Leu Ile 145 150 155 160 Thr Thr Gly Phe Pro Tyr Arg Glu Val Val His Leu Glu Glu Tyr Leu 165 170 175 Glu Ala Leu Gly Arg Val Ile Arg Ala Thr Arg Gly Val Arg Arg Pro 180 185 190 Gly Ala Ala Ser Val Asp Leu Ala Trp Val Ala Cys Gly Arg Phe Asp 195 200 205 Gly Phe Phe Glu Thr Gly Leu Ser Pro Trp Asp Val Ala Ala Gly Ile 210 215 220 Leu Leu Val Glu Glu Gly Gly Gly Arg Val Thr Asp Phe His Gly Arg 225 230 235 240 Pro Asp Pro Ile Phe Ala Arg Gln Met Leu Ala Thr Asn Gly Arg Ile 245 250 255 His Glu Ala Leu Cys Glu Leu Val Ala Pro Leu His His Val Tyr Ala 260 265 270 <210> 165 <211> 219 <212> PRT <213> Slackia heliotrinireducens <400> 165 Met Ile Leu Lys Ala Ala Leu Phe Asp Asn Asp Gly Thr Leu Val Asp 1 5 10 15 Ser Glu Glu Leu Ile Leu Ser Ser Phe Arg Tyr Ala Thr Lys Ser Val 20 25 30 Leu Gly Glu Ala Leu Pro Asp Glu Val Leu Arg Arg Lys Val Gly Gln 35 40 45 Pro Leu Arg Thr Gln Met Ala Asp Phe Thr Pro Asp Val Asp Lys Arg 50 55 60 Glu Glu Leu Phe Arg Val Tyr Gln Glu Phe Asn Ala Arg Glu His Asp 65 70 75 80 Arg Met Ile Arg Leu Phe Pro Asp Val Ala Asn Thr Leu Gly Thr Met 85 90 95 Leu Gln Arg Gly Leu Arg Leu Gly Val Val Thr Ser Lys Leu Ser Glu 100 105 110 Asn Cys Leu Gln Asn Leu Ser His Leu Gly Ile Asp Gly Tyr Phe Glu 115 120 125 Cys Ile Val Ala Pro Asp Asn Cys Pro Leu His Lys Pro Asp Pro Gly 130 135 140 Pro Val Leu Glu Gly Ala Lys Leu Leu Gly Ala Arg Pro Glu Gln Cys 145 150 155 160 Val Tyr Val Gly Asp Ser Pro Tyr Asp Ile Ala Ala Gly Arg Asp Ala 165 170 175 Gly Cys Thr Thr Ile Ala Val Thr Tyr Gly Val Phe Ser Arg Glu Asp 180 185 190 Leu Lys Pro Glu Arg Pro Asp Tyr Phe Cys Asp Ser Phe Ala Glu Leu 195 200 205 Leu Ser Val Leu Asp Gly Ile Val Arg Arg Gly 210 215 <210> 166 <211> 264 <212> PRT <213> Slackia heliotrinireducens <400> 166 Met Leu Ala His Gln Asn Glu Thr Met Thr Lys Tyr Leu Phe Phe Asp 1 5 10 15 Ile Asp Gly Thr Leu Ala Ala Gly Pro Ile Gly Asn Arg Val Val Pro 20 25 30 Glu Gly Thr Arg Asp Ala Leu Leu Arg Leu Arg Lys Asn Gly His Phe 35 40 45 Thr Ala Ile Cys Thr Gly Arg Ser His Ala Met Ala Arg Ser Tyr Met 50 55 60 Glu Glu Gln Gly Phe Thr Asn Met Val Ala Asp Gly Gly Asn Ser Thr 65 70 75 80 Val Ile Asp Gly Asn Leu Leu Gly Ile Glu Pro Leu Asp Arg Lys Arg 85 90 95 Cys Ile Gln Val Leu Glu Glu Cys Asp Arg Leu Lys Ile Pro Trp Ala 100 105 110 Val Ser Ala Asp Asp Ser Asn Thr Arg Leu Ser Ile Asp Gly Arg Phe 115 120 125 Ala Asp Leu Val Glu Lys Gly Tyr Met Val Thr Lys Thr Val Pro Asn 130 135 140 Leu Asp Tyr His Ala Ile Pro Arg Phe Tyr Lys Leu Tyr Ile Ala Val 145 150 155 160 Asn Ala Glu Asp Glu Lys Arg Ile Thr Thr Leu Asp Leu Val Pro His 165 170 175 Val His Phe Ser Asp Thr Cys Leu Phe Val Glu Pro Ile Asp Lys Ser 180 185 190 Arg Gly Ile Met Arg Ile Met Glu His Leu Gly Ala Pro Ile Glu Asp 195 200 205 Val Val Val Phe Gly Asp Gly Met Asn Asp Leu Ser Met Phe Asp Pro 210 215 220 Arg Trp Leu Ser Ile Ala Met Gly Asn Ala Val Gln Pro Leu Lys Asp 225 230 235 240 Ala Ala Asp Tyr Ile Thr Ala Asn Ala Asp Asp Asp Gly Ile Arg Lys 245 250 255 Ala Cys Glu His Phe Gly Trp Ile 260 <210> 167 <211> 238 <212> PRT <213> Staphylothermus marinus <400> 167 Met Ile Arg Leu Ala Ala Phe Asp Ile Asp Gly Thr Leu Thr Ile Asn 1 5 10 15 Arg Ser Ser Thr Val Leu Cys Leu Glu Ala Ile Asp Ala Leu Arg Lys 20 25 30 Leu Glu Lys Asn Gly Val Ile Val Val Leu Val Ser Ser Asn Ala Leu 35 40 45 Pro Val Val Val Gly Leu Lys Lys Tyr Ile Gly Leu Ser Gly Pro Ala 50 55 60 Ile Gly Glu Thr Gly Ala Leu Ile Tyr Tyr Gly Glu Glu Glu Ile Val 65 70 75 80 Ala Thr Thr Lys Tyr Ser Ala Lys Gln Ala Tyr Leu Asp Val Leu Glu 85 90 95 Lys Tyr Asn Glu Tyr Val Tyr Gly Ser Trp Gln Asn Met Phe Arg Leu 100 105 110 His Asp Tyr Ala Leu Lys Ile Arg Lys Gln Tyr Leu Ser Lys Asp Asn 115 120 125 Glu Ile Tyr Ser Leu Ile Lys Glu Tyr Val Glu Asn Lys Tyr Pro Tyr 130 135 140 Ile Lys Val Gly Tyr Ser Gly Tyr Ala Ile His Leu Thr Pro Lys Asp 145 150 155 160 Thr Gly Lys Gly Lys Ala Leu Lys Gln Ile Met Glu Lys His Gly Ile 165 170 175 Arg Arg Glu Glu Thr Met Gly Val Gly Asp Ser Ile Met Asp Trp Glu 180 185 190 Phe Ile Lys Glu Thr Lys Ile Lys Val Ala Val Ala Asn Ala Asp Pro 195 200 205 Glu Leu Arg Arg Lys Ala Asp Ile Val Thr Thr Lys Pro Ser Gly Tyr 210 215 220 Gly Val Val Glu Ile Val Glu Lys Ile Leu Asp Lys Pro Pro 225 230 235 <210> 168 <211> 227 <212> PRT <213> Staphylothermus marinus <400> 168 Met Ala Arg Ile Lys Ala Val Leu Leu Asp Tyr Asp Leu Thr Leu Met 1 5 10 15 Asn Asn Leu Ile Asp Phe Tyr Asp Ala Tyr Asn Glu Ala Leu Lys Lys 20 25 30 Phe Val Gly Lys Thr Leu Gly Phe Asn Glu Phe Phe His Leu Leu Ile 35 40 45 Asn Tyr Ser Leu Gln Ala Tyr Ile Pro Pro Asp Val Asp Arg Phe Ser 50 55 60 Phe Trp Arg Tyr Phe Arg Gln Val Tyr Arg Thr Arg Tyr Gly Tyr Pro 65 70 75 80 Met Glu Gly Ala Tyr Tyr Phe Leu Tyr Trp Val Lys Ala Leu Gly Leu 85 90 95 Lys Thr Ile Ile Val Ser Gly Arg Glu Cys His Glu Ser Ser Ile Trp 100 105 110 Glu Glu Leu Lys Arg Phe Gly Leu Asn Glu Tyr Ile Asp Lys Val Tyr 115 120 125 Thr Met Phe Asp Thr Leu Ile Leu Gly Gly Val Glu Glu Glu Leu Phe 130 135 140 Asp Lys Thr Trp Leu Ile Ser Tyr Ala Leu Ser Ser Tyr Gly Leu Asp 145 150 155 160 Arg Asp Glu Val Val Tyr Ile Gly Asp Tyr Arg Gln Asp Leu Leu Ser 165 170 175 Ser Gln Arg Thr Gly Ile Lys Phe Ile Gly Ile Ala Phe Ser Glu Lys 180 185 190 Arg Lys Glu Cys Leu Arg Arg Leu Gly Ala Glu Tyr Val Gly Ser Asn 195 200 205 Leu Tyr Asp Val Thr Tyr Tyr Leu Trp Gln Ile Ile Arg Glu Val Glu 210 215 220 Phe Lys Lys 225 <210> 169 <211> 222 <212> PRT <213> Staphylothermus marinus <400> 169 Met Thr Ser Thr Glu Val Val Lys Ala Val Leu Phe Asp Met Asp Gly 1 5 10 15 Thr Ile Ile Asn Ser Val Glu Leu Ile Ala Glu Cys Trp Ser Lys Ala 20 25 30 Phe Lys Lys His Gly Ile Arg Ile Glu Pro Gln Asp Ile Tyr Arg Val 35 40 45 Val Gly Leu Pro Ala Asp Thr Ile Leu Glu Lys Tyr Thr Gly Thr Lys 50 55 60 Asn Pro Arg Leu His Asn Ser Ile Leu Glu Gln Ala Arg Lys Cys Phe 65 70 75 80 Glu Glu Lys Met Asn Pro Asn Thr Leu Leu Tyr Asn Asp Val Leu Glu 85 90 95 Thr Ile Lys Gln Leu Arg Glu Asn Asn Lys Leu Cys Gly Ile Val Thr 100 105 110 Ser Ser Ser Cys Lys Arg Thr Ile Glu Leu Leu Glu Lys Leu Asp Ile 115 120 125 Ile Glu Tyr Phe Asp Thr Ile Gln Cys Tyr Gln Gly Lys Leu Arg Gly 130 135 140 Lys Pro Tyr Pro Asp Leu Leu Leu Ser Ala Leu Asn Lys Leu Gly Ile 145 150 155 160 Lys Pro Ser Gln Ala Ile Tyr Val Gly Asp Ser Tyr Ile Asp Tyr Leu 165 170 175 Thr Ala Arg Asn Thr Gly Val Leu Phe Val Leu Val Lys Arg Ser Trp 180 185 190 Asn Thr His Ile Val Asp Lys Cys Ser Glu Lys Cys Ile Val Ile Ser 195 200 205 Asp Leu Arg Glu Ile Ser Asn Leu Ile Thr Asn Lys Ile Lys 210 215 220 <210> 170 <211> 225 <212> PRT <213> Sulfolobus acidocaldarius <400> 170 Met Asn Lys Asp Ile Ile Phe Val Ser Asp Tyr Asp Arg Thr Leu Ser 1 5 10 15 Ser Glu Lys Asn Asn Phe Arg Ile Asp Lys Glu Val Ala Arg Ile Val 20 25 30 Asn Asp Phe Ser Lys Thr Tyr Pro Phe Phe Val Val Thr Gly Arg Glu 35 40 45 Lys Lys Phe Ile Asp Ile Leu Ala Pro Glu Leu Lys Pro Thr Gly Trp 50 55 60 Ile Leu Glu Asn Gly Ala Leu Met Tyr Val Asn Gly Glu Leu Ile Tyr 65 70 75 80 Asn Ile Gln Pro Ser Trp Phe Asp Thr Arg Lys Asn Ile Ile Lys Ile 85 90 95 Leu Asp Asn Tyr Asn Ile Ser Tyr Ser Leu Gly Asn Val Ile Val Tyr 100 105 110 Val Asp Lys Ala His Glu Tyr Lys Gly Val Leu Asn Ser Ile Lys Asp 115 120 125 Ala Thr Val Glu Trp Asn Arg Asn Asp Ala Met Ile Leu Pro Lys Gly 130 135 140 Val Asp Lys Gly Ser Ala Val Ile Gln Leu Arg Glu Arg Leu Gly Tyr 145 150 155 160 Arg Gly Lys Ile Val Ala Ile Gly Asp Ser Glu Asn Asp Ile Ser Met 165 170 175 Phe Arg Val Ala Asp Ile Arg Val Ser Val Ala Asn Ala Leu Pro Met 180 185 190 Ile Lys Glu Ile Ser Glu Leu Ile Leu Glu Lys Glu Asp Gly Glu Gly 195 200 205 Val Lys Glu Phe Leu Leu Lys Val Leu Lys Gly Glu Ile Ile Leu Ile 210 215 220 Lys 225 <210> 171 <211> 224 <212> PRT <213> Sulfolobus acidocaldarius <400> 171 Met Ile Tyr Asn Ile Phe Ile Phe Val Ile Lys Tyr Asn Phe Pro Val 1 5 10 15 Phe Lys Val Lys Gly Val Ile Phe Asp Leu Asp Gly Thr Leu Ala Asn 20 25 30 Thr Ala Leu Ile His Lys Glu Ala Trp Glu Lys Ala Leu Asp Arg Leu 35 40 45 Gly Ile Lys Ser Asp Val Lys Ile Asp Asn Leu Leu Gly Arg Lys Ser 50 55 60 Ser Asp Ile Ala Lys Leu Leu Ala Pro Asn Asn Trp Ser Glu Leu Ile 65 70 75 80 Asn Ile Lys Asn Gln Ile Tyr Val Glu Leu Val Lys Glu Lys Ala Ser 85 90 95 Pro Thr Pro Cys Ala Leu Asp Leu Leu Ser Tyr Leu Arg Lys Lys Glu 100 105 110 Ile Lys Thr Ala Ile Val Thr Ser Ser Asn Lys Leu Ser Ser Gly Ser 115 120 125 Val Leu Lys Lys Leu Gly Ile Thr Ser Glu Val Val Leu Thr Gly Asp 130 135 140 Asp Val Ile Asn Ser Lys Pro Asn Pro Glu Gly Ile Asn Lys Ala Leu 145 150 155 160 Ser Leu Leu Tyr Leu Asp Gly Arg Asp Val Ile Gly Val Gly Asp Thr 165 170 175 Glu Val Asp Val Glu Ala Tyr Tyr Lys Ala Gly Leu Gly Gly Ile Tyr 180 185 190 Leu Val Lys Ser Gly Val Pro Phe Arg Glu Glu Val Val Lys Met Tyr 195 200 205 Gly Gly Ile Val Ile Ser Ser Leu Cys Glu Leu Leu Glu Leu Ile Ser 210 215 220 <210> 172 <211> 267 <212> PRT <213> Sulfolobus acidocaldarius <400> 172 Met Lys Arg Glu Asp Val Glu Lys Val Ala Ser Glu Ala Ser Lys Tyr 1 5 10 15 Ile Tyr Glu Glu Arg Glu Asn Lys Asp Val Asp Arg Val Ile Asn Val 20 25 30 His Gly Asn Asp Val Thr Arg Ile Ile Asp Lys Arg Ser Glu Asp Phe 35 40 45 Ile Val Asp Arg Leu Lys Ser Leu Gly Tyr Asn Ile Leu Ile Val Thr 50 55 60 Glu Glu Ser Gly Val Ile Asp Ser Tyr Gly Lys Asn Tyr Asp Tyr Ile 65 70 75 80 Ala Ile Val Asp Pro Leu Asp Gly Ser Thr Asn Phe Val Ser Gly Ile 85 90 95 Pro Trp Ser Ser Val Ser Ile Ala Ile Tyr Asn Arg Asp Glu Glu Asp 100 105 110 Ile Leu Ser Ser Asn Val Gly Ala Val Ser Ser Ile Phe Thr Pro Tyr 115 120 125 Thr Phe Ser Tyr Asp Glu Gly Ser Ala Tyr Val Asn Gly Val Lys Ile 130 135 140 Ala Glu Ile Lys Lys Pro Glu Lys Ile Leu Leu Leu Ala Tyr Phe Ser 145 150 155 160 Arg Ser Lys Leu Pro Asn Leu Lys Leu Phe Phe Glu Lys Ile Gly Gln 165 170 175 Gly Tyr Lys Ile Arg Ser Leu Gly Ser Ala Ser Leu Asp Met Ile Leu 180 185 190 Val Cys Thr Gly Arg Ala Thr Met Phe Phe Asp Ile Arg Gly Lys Leu 195 200 205 Arg Asn Val Asp Ile Ala Ala Ser Ser Asn Phe Cys Ser Arg Leu Gly 210 215 220 Val Ile Pro Tyr Asp Ile Gly Leu Arg Lys Ile Lys Ser Ser Leu Thr 225 230 235 240 Glu Val Ser Val Val Lys Asp Leu Val Ile Ser Leu Asp Glu Ser Leu 245 250 255 Leu Arg Ser Phe Ser Leu Ala Leu Gln Thr Val 260 265 <210> 173 <211> 272 <212> PRT <213> Thermanaerothrix daxensis <400> 173 Met Thr Thr Thr Pro Ile Val Leu Ser Ile Asp Leu Asp Gly Thr Leu 1 5 10 15 Leu Ile Glu Glu Arg Glu Val His Pro Glu Asp Val His Leu Leu His 20 25 30 His Pro Leu Pro Val Thr Leu Ile Leu Ala Thr Gly Arg Ser Leu Glu 35 40 45 Ser Ala Arg Arg Val Leu Glu Arg Ala Gly Leu Tyr Arg His Glu Pro 50 55 60 Leu Pro Phe Pro Leu Val Leu Gln Asn Gly Ala Ala Ile Tyr Arg Pro 65 70 75 80 Gln Glu Val Leu Glu Asp Tyr Phe Pro Ile Pro Pro Glu Ala Glu Thr 85 90 95 Gln Leu His Ala Leu Ala Pro Gln Phe Ser Glu Ala Ala Leu Leu Trp 100 105 110 Gln Gly Leu Asp Gly Ala Tyr Leu Glu Glu Ile Thr Pro Phe Gly Leu 115 120 125 Gln Ala Ala Gly Arg Phe Gly Phe Leu Pro Arg Pro Leu Ser Glu Ala 130 135 140 Pro Ala Gly Gln Gly Phe Gly Lys Leu Met Cys Leu Ser Asp Arg Pro 145 150 155 160 Glu Val Leu Lys Ala Phe Ala Ala Ala Thr Arg His Leu Pro Val Glu 165 170 175 Gly Ser Tyr Ser Leu Asp Val Ile Tyr Glu Val Thr Arg Gln Gly Val 180 185 190 His Lys Gly Phe Gly Leu Arg Ile Leu Leu Gln Arg Leu Gly Leu Thr 195 200 205 His Ala Arg Leu Val Ala Val Gly Asp Gly Asp Asn Asp Val Pro Met 210 215 220 Phe Ser Cys Ala Asp Leu Ala Ile Ala Pro Leu Asn Ala Ser Pro Leu 225 230 235 240 Ala Arg Ser His Ala His Arg Leu Ile Asp Arg Arg Pro His Gly Leu 245 250 255 Leu Thr Pro Val Leu Asp Leu Leu Arg Gln Glu Gly Trp Leu Asp Pro 260 265 270 <210> 174 <211> 215 <212> PRT <213> Thermoanaerobacter sp. <400> 174 Met Asn Val Lys Ala Val Val Phe Asp Leu Asp Gly Thr Leu Ile Asp 1 5 10 15 Ser Lys Lys Asp Ile Ala Met Ala Ala Asn Lys Thr Leu Ser Glu Leu 20 25 30 Gly Leu His Thr Leu Pro Glu Asp Thr Ile Ala Ser Phe Ile Gly Tyr 35 40 45 Gly Gly Glu Leu Phe Ile Lys Arg Cys Ile Gly Glu Lys Asn Ile Asp 50 55 60 Lys Phe Glu Glu Ala Phe Lys Lys Phe Lys Glu Asn Tyr Ser Glu Leu 65 70 75 80 Cys Ile Ile His Thr Ser Leu Phe Pro Gly Val Glu Glu Val Leu Glu 85 90 95 Phe Leu Lys Lys Arg Lys Ile Asn Ile Ala Leu Ala Thr Asn Lys Met 100 105 110 Ile Ser Leu Ser Lys Lys Ile Leu Lys His Leu Glu Val Glu Lys Tyr 115 120 125 Phe Ser Ile Met Leu Gly Pro Glu Asp Val Thr Asn Arg Lys Pro His 130 135 140 Pro Glu Ile Ile Glu Ile Leu Leu Gln Asn Leu Asn Val Arg Pro Glu 145 150 155 160 Glu Thr Leu Tyr Val Gly Asp Ser Glu Ile Asp Val Phe Cys Gly Lys 165 170 175 Ser Ala Gly Val Tyr Thr Cys Ala Val Thr Tyr Gly Ile Gly Asp Ile 180 185 190 Lys Ser Ile Ile Ala Ala Asp Pro Asp Phe Ile Ile Thr Asp Leu Thr 195 200 205 Lys Leu Ile Ile Leu Leu Ser 210 215 <210> 175 <211> 273 <212> PRT <213> Thermoanaerobacter thermohydrosulfuricus <400> 175 Met Tyr Lys Leu Phe Val Thr Asp Ala Asp Gly Ser Leu Leu Asn Ser 1 5 10 15 Asn Ser Glu Ile Ser Asp Lys Asn Lys Glu Ala Ile Lys Glu Val Ile 20 25 30 Ser Arg Gly Val Ile Phe Thr Ile Ala Thr Gly Arg Met Phe Ser Ser 35 40 45 Ile Leu Pro Tyr Ala Phe Glu Leu Asn Val Asn Ala Pro Val Ile Ser 50 55 60 Tyr Asn Gly Ala Leu Ile Lys Asp Ile Tyr Thr Lys Lys Val Tyr Tyr 65 70 75 80 Tyr Asn Pro Ile Gln Thr Glu Asp Ala Ile Phe Ala Ile Arg Leu Leu 85 90 95 Lys Glu Ser Gly Tyr His Ile Asn Leu Tyr Ile Asp Asp Glu Leu Tyr 100 105 110 Val Glu Glu Ile Thr Asp Arg Val Glu Trp Tyr Leu Ser Phe Asn Asn 115 120 125 Val Thr Val Asn Ala Val Gly Asn Leu Glu Glu Phe Leu Lys Arg Thr 130 135 140 Gly Gly Val Thr Ala Lys Ile Tyr Ala Ile Asn Asp Met Lys Asn Pro 145 150 155 160 Ile Ser Ile Asp Ala Glu Ile Tyr Asp Glu Ile Ser Lys Arg Leu Thr 165 170 175 Ile Ser Thr Ser Gly Gly Gly His Leu Glu Ile Asn Ala Lys Gly Val 180 185 190 Ser Lys Gly Asn Ala Leu Lys Thr Leu Ala Asn Met Tyr Ser Ile Lys 195 200 205 Arg Glu Gln Val Val Ala Ile Gly Asp Asn Leu Asn Asp Leu Ser Met 210 215 220 Ile Glu Tyr Ala Gly Leu Gly Val Ala Met Gly Asn Ala Pro Asp Ile 225 230 235 240 Val Lys Ile Lys Ala Asp Tyr Thr Thr Leu Ser Asn Asp Glu Asp Gly 245 250 255 Val Ala His Val Ile Asp Lys Phe Phe Leu Asn Lys Lys Thr Val Ala 260 265 270 Val <210> 176 <211> 273 <212> PRT <213> Thermoanaerobacter thermohydrosulfuricus <400> 176 Met Tyr Lys Leu Phe Val Thr Asp Ala Asp Gly Ser Leu Leu Asn Ser 1 5 10 15 Asn Ser Glu Ile Ser Asp Lys Asn Lys Glu Glu Ile Lys Glu Val Ile 20 25 30 Ser Arg Gly Val Ile Phe Thr Ile Ala Thr Gly Arg Met Phe Ser Ser 35 40 45 Ile Leu Pro Tyr Ala Phe Glu Leu Asn Val Asn Ala Pro Val Ile Ser 50 55 60 Tyr Asn Gly Ala Leu Ile Lys Asp Ile Tyr Thr Lys Lys Val Tyr Tyr 65 70 75 80 Tyr Asn Pro Ile Gln Thr Glu Asp Ala Ile Phe Ala Ile Arg Leu Leu 85 90 95 Lys Glu Ser Gly Tyr His Ile Asn Leu Tyr Ile Asp Asp Glu Leu Tyr 100 105 110 Val Glu Glu Ile Thr Asp Arg Val Glu Trp Tyr Leu Ser Phe Asn Asn 115 120 125 Val Thr Val Asn Ala Val Gly Asn Leu Glu Glu Phe Leu Lys Arg Thr 130 135 140 Gly Gly Val Thr Ala Lys Ile Tyr Ala Ile Asn Asp Met Lys Asn Pro 145 150 155 160 Ile Ser Ile Asp Ala Glu Ile Tyr Asp Glu Ile Ser Lys Arg Leu Thr 165 170 175 Ile Ser Thr Ser Gly Gly Gly His Leu Glu Ile Asn Ala Lys Gly Val 180 185 190 Ser Lys Gly Asn Ala Leu Lys Thr Leu Ala Asn Met Tyr Ser Ile Lys 195 200 205 Arg Glu Gln Val Val Ala Ile Gly Asp Asn Leu Asn Asp Leu Ser Met 210 215 220 Ile Glu Tyr Ala Gly Leu Gly Val Ala Met Gly Asn Ala Pro Asp Ile 225 230 235 240 Val Lys Ile Lys Ala Asp Tyr Thr Thr Leu Ser Asn Asp Glu Asp Gly 245 250 255 Val Ala His Val Ile Asp Lys Phe Phe Leu Asn Lys Lys Thr Val Ala 260 265 270 Val <210> 177 <211> 257 <212> PRT <213> Thermoanaerobacter wiegelii <400> 177 Met Lys Asp Glu Lys Gly Ile Val Val Asp Ile Ile Lys Lys Ala Gly 1 5 10 15 Glu Ile Leu Gln Asp Gly Trp Asn Lys Lys Asn Phe Lys Ile Tyr Arg 20 25 30 Lys Gly Thr Ile Asn Leu Val Thr Glu Ile Asp Lys Lys Ile Glu Phe 35 40 45 Leu Ile Ile Gln Leu Leu Lys Gln Tyr Phe Pro Asp Tyr Gly Ile Leu 50 55 60 Thr Glu Glu Ser Lys Glu Ile Asn Ser Lys Ala Asn Val Arg Trp Ile 65 70 75 80 Ile Asp Pro Leu Asp Gly Thr Thr Asn Tyr Ile Lys Gln Tyr Pro Phe 85 90 95 Val Ala Ile Ser Ile Ala Leu Glu Lys Glu Gly Glu Leu Ile Leu Gly 100 105 110 Val Val Tyr Asn Pro Ile Leu Asn Glu Met Phe Ile Ala Gln Lys Gly 115 120 125 Cys Gly Ala Thr Tyr Asn Gly Lys Ser Ile His Val Ser Lys Ile Lys 130 135 140 Glu Leu Gly Ser Ala Val Leu Ala Ser Gly Phe Pro Tyr Asp Ala Trp 145 150 155 160 Glu Asn Pro Asp Asn Asn Ala Lys Gln Trp Arg Gln Phe Leu Thr Arg 165 170 175 Ser Leu Ser Leu Arg Cys Asp Gly Ser Ala Ala Leu Asp Leu Cys Arg 180 185 190 Val Ala Cys Gly Gln Leu Asp Gly Tyr Trp Glu Lys Gly Ile Ser Pro 195 200 205 Trp Asp Val Ala Ala Gly Ile Val Ile Leu Arg Glu Ala Gly Gly Ile 210 215 220 Ile Thr Asp Tyr Leu Gly Glu Glu Asn Phe Phe Lys Arg Gly Glu Val 225 230 235 240 Val Ala Ala Asn Pro Val Leu His Ala Gln Met Leu Lys Val Leu Asn 245 250 255 Asn <210> 178 <211> 215 <212> PRT <213> Thermoanaerobacter wiegelii <400> 178 Met Asn Val Lys Thr Ile Ile Phe Asp Leu Asp Gly Thr Leu Ile Asp 1 5 10 15 Ser Lys Lys Asp Ile Val Met Ala Ile Asn Lys Thr Leu Arg Asp Leu 20 25 30 Asp Ile Pro Thr Leu Pro Glu Asp Asp Ile Ile Pro Phe Met Ser Tyr 35 40 45 Gly Pro Glu Val Phe Ile Lys Gln Cys Ile Gly Glu Lys Asn Ala Asp 50 55 60 Lys Phe Glu Arg Ala Phe Glu Lys Phe Lys Glu Asn Tyr Ser Glu Arg 65 70 75 80 Cys Ile Val Tyr Thr Ser Leu Phe Pro Gly Val Arg Glu Val Leu Glu 85 90 95 Phe Leu Lys Glu Arg Lys Ile Asn Ile Ala Leu Ala Thr Asn Lys Met 100 105 110 Met Ser Leu Ser Lys Lys Ile Leu Gln His Phe Gly Leu Glu Lys Tyr 115 120 125 Phe Ser Ile Met Leu Gly Pro Glu Asp Val Thr Asn Lys Lys Pro His 130 135 140 Pro Glu Ile Ile Glu Ile Ile Leu Gln Lys Leu Asn Val Lys Arg Glu 145 150 155 160 Glu Ala Leu Tyr Val Gly Asp Ser Glu Ile Asp Val Leu Cys Gly Lys 165 170 175 Ser Ala Gly Val Tyr Thr Cys Ala Val Thr Tyr Gly Ile Gly Asp Ile 180 185 190 Lys Ser Ile Ile Ala Ala Asn Pro Asp Phe Ile Ile Thr Asp Leu Thr 195 200 205 Lys Leu Ile Leu Leu Val Ser 210 215 <210> 179 <211> 205 <212> PRT <213> Thermoanaerobacterium xylanolyticum <400> 179 Met Ser Thr Arg Leu Phe Ile Val Arg His Gly Glu Thr Leu Trp Asn 1 5 10 15 Arg Gln Lys Lys Ile Gln Gly Ala Ser Asp Thr Gln Leu Ser Asp Glu 20 25 30 Gly Met Lys Gln Ala Tyr Leu Leu Ser Gln Arg Leu Lys Asn Glu Ile 35 40 45 Ile Asp Val Ile Phe Ser Ser Asp Leu Asp Arg Ala Tyr Lys Thr Ala 50 55 60 Thr Phe Ile Ala Lys Asn Phe Asn Leu Asp Val Ile Lys Leu Pro Glu 65 70 75 80 Leu Arg Glu Ile Ser Phe Gly Val Trp Glu Gly Leu Thr Val Asp Glu 85 90 95 Ile Glu Lys Ser Tyr Lys Glu Leu Tyr His Thr Trp Lys Thr Asn Pro 100 105 110 Pro Glu Ala Thr Ile Glu Gly Ala Glu Thr Leu Lys Ala Val Gln Asp 115 120 125 Arg Ile Leu Asn Ala Thr Asn Lys Ile Ile Glu Gln Tyr Lys Asn Lys 130 135 140 Asn Ile Leu Ile Val Ser His Gly Thr Thr Ile Lys Ala Leu Ile Leu 145 150 155 160 Gly Met Leu Asn Leu Asp Leu Ser Phe Tyr Pro Lys Ile Arg Gln Asp 165 170 175 Asn Thr Ala Leu Asn Ile Ile Asp Val Lys Asp Asp Gly Asn Cys Val 180 185 190 Leu Val Leu Leu Asn Asp Thr Cys His Leu Arg Glu Arg 195 200 205 <210> 180 <211> 595 <212> PRT <213> Thermobifida halotolerans <400> 180 Met Arg Tyr His Val Leu Ala Cys Asp Tyr Asp Glu Thr Ile Ala His 1 5 10 15 Gly Gly Arg Val Asp Asp Ala Thr Val Thr Ala Leu Glu His Leu Ser 20 25 30 Arg Ser Gly Arg Arg Leu Val Met Val Thr Gly Arg Arg Leu Asp Asp 35 40 45 Leu Phe Gly Val Phe Asp His Val Asp Leu Phe Asp Met Val Val Ala 50 55 60 Glu Asn Gly Gly Val Leu Tyr Asp Pro Gly Arg Arg Asp Arg Arg Pro 65 70 75 80 Leu Ala Glu Arg Pro Pro Ala Glu Phe Val Glu Arg Leu Arg Arg His 85 90 95 Gly Val Ser Pro Leu Ala Val Gly Glu Val Val Val Ala Thr Arg Glu 100 105 110 Pro His Asp Arg Thr Val Leu Asp Ala Ile Arg Glu Leu Gly Leu Glu 115 120 125 Leu Gln Val Ile Tyr Asn Lys Gly Ala Val Met Val Leu Pro Pro Gly 130 135 140 Val Asn Lys Ala Ser Gly Leu Ala Ala Ala Leu Arg Arg Leu Glu Phe 145 150 155 160 Ser Pro His Ser Thr Val Ala Val Gly Asp Ala Glu Asn Asp His Ala 165 170 175 Met Leu His Met Cys Glu Cys Ala Val Ala Val Ala Gly Ala Leu Glu 180 185 190 Ala Val Lys Arg Thr Ser Asp Leu Val Leu Glu Arg Pro Asp Gly Glu 195 200 205 Gly Val Ala Glu Leu Val Asp Arg Leu Leu Ala Thr Asp Leu Ala Glu 210 215 220 Val Pro Val Ser Arg His Asp Leu His Phe Gly Thr Ala Gly Ala Ala 225 230 235 240 Pro Thr Ala Ile Ala Pro His Gly Arg Gly Gly Ala Val Ala Gly Pro 245 250 255 Ser Gly Ser Gly Lys Ser Thr Ala Ala Thr Ala Leu Leu Glu Arg Leu 260 265 270 Gly Glu Ser Gly Tyr Gln Tyr Cys Val Ile Asp Pro Glu Gly Asp Tyr 275 280 285 Ala Asp Phe Glu Gly Val Thr Ala Phe Gly Asp Ala Thr Arg Ala Pro 290 295 300 Ser Ile Asp Glu Val Leu Thr Ala Leu Arg Asp Pro Asp His Asn Val 305 310 315 320 Ala Val Asn Met Leu Ala Val Pro Leu Arg Asp Arg Pro Ala Phe Phe 325 330 335 Ala Gly Leu Leu Pro Arg Leu Val Gly Leu Arg Ala Glu Leu Gly His 340 345 350 Pro Ala Trp Leu Val Val Asp Glu Ala His His Leu Met Pro Val Glu 355 360 365 Leu Ala Gln Leu Pro Leu Arg Val Pro Ala Asp Val Gly Gly Leu Met 370 375 380 Leu Val Thr Val His Pro Glu Thr Leu Ser Pro Leu Val Leu Arg Leu 385 390 395 400 Val Asp Thr Val Val Ala Val Gly Asp Asp Pro Gly Gln Thr Leu Asp 405 410 415 Ala Phe Ala Ala Ala Thr Gly His Arg Pro Pro Ala Leu Glu Pro Ala 420 425 430 Pro Ala Glu Pro Asp Arg Gln Gln Glu Ser Val Val Ser Asp Ile Ala 435 440 445 Val Trp Arg Val Ala Glu Thr Ala Ala Gln Arg Val Lys Leu Ala Pro 450 455 460 Gly Glu Thr His Arg Thr Arg His Arg Arg Lys Tyr Ala Ala Gly Thr 465 470 475 480 Leu Ala Pro Asp Lys Ser Phe Tyr Phe Thr Gly Pro Gln Gly Arg Leu 485 490 495 Arg Leu Arg Ala Arg Asn Leu His Ser Phe Val Glu Leu Ala Glu Gly 500 505 510 Val Asp Asp Asp Thr Trp Thr His His Leu Arg Arg His Asp Tyr Ser 515 520 525 Arg Trp Leu Arg Gly Ser Val Arg Asp Gly Glu Leu Ala Asp Leu Val 530 535 540 Arg Arg Val Glu Gln Asp Thr Gly Arg Ala Pro Ser Asp Ser Arg Arg 545 550 555 560 Glu Val Ala Arg Leu Ile Asp Glu Arg Tyr Thr Leu Pro Ala Glu Pro 565 570 575 Thr Ser Tyr Asp Pro Asp Arg Gly Arg Glu Thr Gly Pro Ser Gly Arg 580 585 590 Arg Arg Pro 595 <210> 181 <211> 237 <212> PRT <213> Thermobifida halotolerans <400> 181 Met Gly Leu Arg Glu Thr Asp Thr Glu Phe Val Pro Pro Arg Ala Ala 1 5 10 15 Leu Phe Asp Leu Asp Gly Thr Leu Ile Asn Ser Glu Pro Arg Ser Ala 20 25 30 Ala Val Trp Ala Arg Leu Leu Arg Ala Arg Gly Ile Thr Pro Asp Glu 35 40 45 Ala Leu Leu Arg Arg Phe Met Gly Arg Arg Gly Gly Asp Val Val Ala 50 55 60 Glu Leu Pro His Leu Phe Pro Gly Glu Ser Leu Glu Gln Ile Phe Asp 65 70 75 80 Glu Leu Trp Arg His Gly Gln Asp Pro Asp Leu Pro Gln Val Ala Pro 85 90 95 Leu Pro Glu Ser Val Ala Phe Leu His His Leu His Ser Gln Gly Val 100 105 110 Pro Phe Ala Leu Val Thr Ser Ala Gly Arg Glu Trp Ala Glu Ser Ala 115 120 125 Leu Glu Trp Leu Gly Val Arg Gly Met Phe Arg Gly Leu Val Thr Ala 130 135 140 Gly Asp Val Thr Val Gly Lys Pro His Pro Gln Gly Tyr Leu Gly Gly 145 150 155 160 Ala Glu Ile Leu Gly His Glu Pro Glu His Ile Val Val Phe Glu Asp 165 170 175 Thr Pro Ala Gly Ile Met Ala Gly Arg Gly Ala Gly Met Arg Val Val 180 185 190 Gly Ile Thr Thr Thr His Pro Pro Glu Ala Leu Thr Gln Ala Asp Leu 195 200 205 Val Val Glu His Leu Thr Gln Val Ala Trp Pro Arg Leu Ser Leu Arg 210 215 220 Asp Pro Glu Pro Pro Arg Ser Ser Phe Ala Gly Arg Gly 225 230 235 <210> 182 <211> 236 <212> PRT <213> Thermococcus celer <400> 182 Met Gly Arg Val Lys Ala Ile Ser Leu Asp Ile Asp Gly Thr Ile Thr 1 5 10 15 Tyr Pro Asp Arg Arg Leu His Glu Glu Ala Met Arg Ala Ile Arg Leu 20 25 30 Ala Glu Ser Leu Gly Val Pro Val Met Leu Val Thr Gly Asn Ser Val 35 40 45 Pro Phe Ala Glu Ala Met Ala Ile Met Ile Gly Thr Ser Gly Pro Val 50 55 60 Val Ala Glu Asp Gly Gly Ala Leu Ser Ile Lys Asp Gly Arg Leu Arg 65 70 75 80 Lys Arg Val Tyr Leu Thr Thr Met Asp Glu Glu Trp Ile Leu Trp Ser 85 90 95 Glu Val Lys Lys Arg Tyr Pro Gly Ala Ile Leu Ser Phe Ser Met Pro 100 105 110 Glu Arg Lys Ala Gly Leu Val Ile Leu Arg Thr Val Pro Val Glu Ala 115 120 125 Val Arg Glu Leu Ile Lys Glu Leu Gly Leu Asn Leu Ile Ala Val Asp 130 135 140 Ser Gly Phe Ala Ile His Val Lys Lys Pro Trp Ile Asn Lys Gly Thr 145 150 155 160 Gly Ile Glu Lys Ala Cys Glu Val Leu Gly Ile Lys Pro Arg Glu Val 165 170 175 Ala His Val Gly Asp Gly Glu Asn Asp Leu Asp Ala Phe Arg Val Val 180 185 190 Gly Tyr Arg Val Ala Val Gly Gln Ala Pro Glu Ser Leu Lys Gly Glu 195 200 205 Ala Asp Tyr Val Thr Glu Lys Thr Tyr Gly Ala Gly Gly Ala Glu Gly 210 215 220 Ile Leu His Val Leu Arg Glu Phe Gly Tyr Met Glu 225 230 235 <210> 183 <211> 236 <212> PRT <213> Thermococcus celer <400> 183 Met Gly Arg Val Lys Ala Ile Ser Leu Asp Ile Asp Gly Thr Ile Thr 1 5 10 15 Tyr Pro Asp Arg Arg Leu His Glu Glu Ala Met Arg Ala Ile Arg Leu 20 25 30 Ala Glu Ser Leu Gly Val Pro Val Met Leu Val Thr Gly Asn Ser Val 35 40 45 Pro Phe Ala Glu Ala Met Ala Ile Met Ile Gly Thr Ser Gly Pro Val 50 55 60 Val Ala Glu Asp Gly Gly Ala Leu Ser Ile Lys Asp Gly Arg Leu Arg 65 70 75 80 Lys Arg Val Tyr Leu Thr Thr Met Asp Glu Glu Trp Ile Leu Trp Ser 85 90 95 Glu Val Lys Lys Arg Tyr Pro Gly Ala Ile Leu Ser Phe Ser Met Pro 100 105 110 Glu Arg Lys Ala Gly Leu Val Ile Leu Arg Thr Val Pro Val Glu Ala 115 120 125 Val Arg Glu Leu Ile Lys Glu Leu Gly Leu Asn Leu Ile Ala Val Asp 130 135 140 Ser Gly Phe Ala Ile His Val Lys Lys Pro Trp Ile Asn Lys Gly Thr 145 150 155 160 Gly Ile Glu Lys Ala Cys Glu Val Leu Gly Ile Lys Pro Arg Glu Val 165 170 175 Ala His Val Gly Asp Gly Glu Asn Asp Leu Asp Ala Phe Arg Val Val 180 185 190 Gly Tyr Arg Val Ala Val Gly Gln Ala Pro Glu Ser Leu Lys Gly Glu 195 200 205 Ala Asp Tyr Val Thr Glu Lys Thr Tyr Gly Ala Gly Gly Ala Glu Gly 210 215 220 Ile Leu His Val Leu Arg Glu Phe Gly Tyr Met Glu 225 230 235 <210> 184 <211> 251 <212> PRT <213> Thermococcus litoralis <400> 184 Met Tyr Glu Trp Asn Glu Ile Ala Leu Asn Leu Ala Lys Asp Ile Glu 1 5 10 15 Arg Glu Val Met Pro Leu Phe Gly Thr Lys Lys Ala Gly Glu Phe Ile 20 25 30 Gly Phe Ser Pro Ser Gly Asp Lys Thr Lys Leu Val Asp Lys Val Ala 35 40 45 Glu Asp Val Val Leu Glu Tyr Leu Arg Pro Leu Gly Val Asn Val Val 50 55 60 Ser Glu Glu Ile Gly Asn Ile Glu Ala Gly Ser Glu Tyr Thr Ile Val 65 70 75 80 Val Asp Pro Ile Asp Gly Ser Phe Asn Phe Ile Gln Gly Ile Pro Ile 85 90 95 Phe Gly Phe Ser Phe Ala Val Phe Lys Asn Glu Lys Pro Val Tyr Ala 100 105 110 Met Ile Tyr Glu Phe Ile Thr Lys Asn Val Tyr Glu Gly Ile Pro Gly 115 120 125 Glu Gly Ala Tyr Leu Asn Gly Glu Arg Ile Arg Val Arg His Leu Asn 130 135 140 Glu Lys Ser Ile Ser Ile Ser Phe Tyr Thr Arg Gly Arg Gly Ala Arg 145 150 155 160 Leu Val Glu Lys Val Lys Arg Thr Arg Val Leu Gly Ala Ile Ala Val 165 170 175 Glu Leu Ala Tyr Leu Ala Arg Gly Ser Leu Asp Gly Val Ile Asp Ile 180 185 190 Arg Asn Tyr Val Arg Pro Thr Asp Ile Ala Ala Gly Tyr Ile Ile Ala 195 200 205 Lys Glu Ala Gly Ala Ile Ile Thr Asp Asp Ser Gly Glu Glu Ile Lys 210 215 220 Phe Arg Leu Asp Ala Arg Glu Lys Leu Asn Ile Ile Ala Val Asn Asp 225 230 235 240 Lys Arg Leu Leu Lys Leu Ile Leu Glu Val Ile 245 250 <210> 185 <211> 237 <212> PRT <213> Thermococcus profundus <400> 185 Met Lys Val Arg Ala Ile Ser Leu Asp Ile Asp Gly Thr Ile Thr Tyr 1 5 10 15 Arg Asp Arg Arg Leu Ser Ile Glu Ala Leu Lys Ala Ile Arg Leu Ala 20 25 30 Glu Ser Leu Gly Val Pro Val Met Leu Val Thr Gly Asn Ser Val Pro 35 40 45 Phe Ala Glu Ala Ala Ala Ile Phe Ile Gly Thr Ser Gly Pro Val Ile 50 55 60 Ala Glu Asp Gly Gly Ala Leu Ser Leu Lys Gly Lys Gly Thr Met Arg 65 70 75 80 Lys Arg Val Phe Leu Thr Asp Met Asp Glu Glu Trp Ile Leu Trp Ser 85 90 95 Glu Leu Lys Lys Arg Tyr Pro Lys Ala Glu Leu Ser Phe Ser Thr Met 100 105 110 Glu Arg Lys Ala Gly Leu Val Ile Arg Arg Thr Ile Pro Val Glu Ala 115 120 125 Val Arg Glu Ile Ile Arg Glu Leu Gly Leu Asn Leu Val Ala Val Asp 130 135 140 Ser Gly Phe Ala Ile His Val Lys Lys Pro Trp Ile Asn Lys Gly Thr 145 150 155 160 Gly Ile Glu Lys Ala Cys Glu Tyr Leu Gly Ile Ser Pro Lys Glu Val 165 170 175 Ala His Val Gly Asp Gly Glu Asn Asp Leu Asp Ala Phe Arg Val Val 180 185 190 Gly Tyr Arg Val Ala Ile Ala Gln Ala Pro Glu Ser Val Lys Glu Lys 195 200 205 Ala Asp Tyr Val Thr Gln Lys Pro Tyr Gly Asp Gly Gly Ala Glu Ala 210 215 220 Val Met His Ile Leu Arg Lys Phe Gly Tyr Leu Glu Glu 225 230 235 <210> 186 <211> 215 <212> PRT <213> Thermocrinis minerva <400> 186 Met Phe Lys Leu Val Ile Phe Asp Leu Asp Gly Thr Leu Ile Asp Ser 1 5 10 15 Cys Gln Asp Ile Thr Leu Cys Leu Asn Met Thr Leu Arg Asp Phe Gly 20 25 30 Lys Pro Gln Val Glu Ser Gln Thr Val Arg Arg Tyr Ile Gly Ser Gly 35 40 45 Ala Arg Ala Leu Leu Glu Lys Phe Phe Pro Pro Glu Asp Val Asp Lys 50 55 60 Ala Leu Glu Val Phe Arg Gly Tyr Tyr Arg Gln His Pro Val Val Tyr 65 70 75 80 Thr Arg Pro Tyr Glu Gly Ile Pro Glu Ala Leu Glu Phe Leu Arg Ser 85 90 95 Lys Gly Val Ile Met Thr Val Val Thr Asn Lys Met Glu Glu Ile Ser 100 105 110 Tyr Lys Ile Leu Asp Thr Leu Lys Leu Leu Asp Tyr Phe Ser Leu Val 115 120 125 Val Gly Gly Asp Thr Tyr His Glu Lys Lys Pro Ser Ala Leu Pro Ile 130 135 140 Ile Lys Thr Leu Glu Lys Phe Ser Val Lys Pro Lys Glu Ser Leu Ile 145 150 155 160 Val Gly Asp Thr Glu Ala Asp Ile Leu Ser Gly Lys Arg Ala Ser Val 165 170 175 Tyr Thr Ala Leu Ala Leu Trp Gly Tyr Thr Lys Asp Met Gln Glu Glu 180 185 190 Pro Asp Phe Ile Leu Asn Thr Pro Thr Glu Ile Ile Glu Leu Trp Asn 195 200 205 Ser Arg Ser Thr Ser Arg Val 210 215 <210> 187 <211> 404 <212> PRT <213> Thermocrinis ruber <400> 187 Met Glu Gly Gly Ser Phe Leu Asn Leu Ile Glu Ser Leu Asp Lys Phe 1 5 10 15 Ala Val Gly Ile Phe Arg Gln Phe Leu Ile Arg Glu Asp Thr Asp Lys 20 25 30 Ile Leu Ile Leu Thr Pro Asn Glu Glu Tyr Lys Arg Trp Ala Ile Ala 35 40 45 Tyr Leu Ser Thr Arg Leu Lys Gly Phe Gly Lys Lys Val Val Val Glu 50 55 60 Cys Glu Gly Glu Arg Lys Glu Gln Lys Glu Ile Glu Ser Pro Lys Asp 65 70 75 80 Asn Leu Leu Asp Lys Tyr Thr Phe Glu Asn Phe Val Val Gly Pro Ser 85 90 95 Asn Glu Leu Ala Tyr Lys Val Cys Leu Glu Val Thr Lys Asn Pro Gly 100 105 110 Lys Leu Phe Asn Pro Leu Phe Ile Tyr Gly Arg Ser Gly Leu Gly Lys 115 120 125 Thr His Leu Leu His Ala Ile Gly Asn Gln Leu Lys Ala Arg Tyr Arg 130 135 140 Val Leu Tyr Ile Pro Leu Met Asp Phe Ser Asp Ser Met Val Lys Ala 145 150 155 160 Phe Lys Glu Asn Arg Val Glu Glu Phe Arg Glu Arg Phe Phe Asn Leu 165 170 175 Asp Val Leu Leu Leu Asp Asp Val Gln Phe Leu Val Gly Lys Glu Arg 180 185 190 Thr Gln Ile Glu Leu Phe Arg Ile Tyr Glu Lys Leu Gln Ala Glu Glu 195 200 205 Lys Gln Ile Val Leu Val Ser Asp Arg His Pro Arg Asp Leu Lys Asp 210 215 220 Val Ser Glu Arg Leu Val Ser Arg Phe Glu Ser Gly Leu Ile Ile Glu 225 230 235 240 Ile Gly Leu Asp Glu Glu Thr Lys Arg Arg Ile Ile Lys Gln Lys Leu 245 250 255 Ile Leu Tyr Gly Leu Pro Leu Asp Gln Glu Thr Ile Asp Tyr Val Phe 260 265 270 Glu Asn Thr Gly Tyr Asn Val Arg Glu Ile Glu Gly Phe Val Lys Thr 275 280 285 Leu Lys Val Ser Gly Ile Lys Arg Leu Pro Lys Ser Glu Glu Leu Asp 290 295 300 Lys Glu Lys Lys Val Gln Leu Ile Ile Asn Thr Val Ala Lys Gly Phe 305 310 315 320 Lys Leu Asn Pro Glu Leu Leu Lys Lys Asp Thr Lys Glu Arg Arg Met 325 330 335 Ile Asn Ala Arg His Ile Ala Met Phe Leu Cys Lys Thr Ile Leu Asn 340 345 350 Leu Pro Tyr Ser Gln Ile Gly Glu Phe Phe Gly Lys Lys Asp His Thr 355 360 365 Ala Val Met Tyr Ala Val Lys Lys Val Glu Gln Arg Cys Arg Glu Asp 370 375 380 Arg Lys Phe Met Tyr Met Val Ser Phe Phe Glu Lys Ser Ile Arg Lys 385 390 395 400 Ser Leu Gly Leu <210> 188 <211> 222 <212> PRT <213> Thermoflexus hugenholtzii <400> 188 Met Ser Arg Gly Pro Ala Phe Leu Phe Asp Leu Asp Gly Thr Leu Val 1 5 10 15 Asp Ser Val Tyr Gln His Val Leu Ala Trp Arg Glu Ala Leu Glu Ala 20 25 30 Val Gly Ile Ser Leu Ser Val Trp Arg Ile His Arg Arg Ile Gly Met 35 40 45 Ser Gly Gly Leu Leu Leu Arg Ala Leu Leu Arg Glu Thr Gly Arg Glu 50 55 60 Val Thr Ser Glu Glu Val Gln Arg Ile Gln Gln Gln His Ala Glu Ala 65 70 75 80 Phe Ala Arg Leu Leu Pro Gln Val Arg Pro Leu Pro Gly Ala Arg Glu 85 90 95 Leu Leu Glu Ala Leu Thr Arg Ala Gly Ile Pro Trp Ala Ile Ala Thr 100 105 110 Ser Gly Arg Arg Asp Val Ala Arg Pro Leu Leu Glu Leu Leu Gly Val 115 120 125 Asp Glu Gly Ile Pro Val Ile Thr Arg Glu Asp Val Pro Tyr Ala Lys 130 135 140 Pro Asp Pro Asp Leu Phe Leu Ala Ala Ala Ala Arg Leu Gly Ile Pro 145 150 155 160 Ile Ala Glu Cys Ile Val Val Gly Asp Ser Val Trp Asp Leu Leu Ala 165 170 175 Ala Arg Arg Ala Gly Ala Leu Ser Val Gly Leu Leu Ser Gly Gly Tyr 180 185 190 Gly Arg Glu Glu Leu Glu Arg Ala Gly Ala Tyr Arg Val Tyr Glu Asp 195 200 205 Pro Ala Asp Leu Leu Arg His Leu Asp Glu Val Gly Val Arg 210 215 220 <210> 189 <211> 255 <212> PRT <213> Thermotoga lettingae <400> 189 Met Asp Arg Met Asp Phe Ser Ile Lys Ile Ala Arg Lys Val Gly Leu 1 5 10 15 Tyr Leu Met Glu His Trp Gly Asn Ala Glu Asn Val Arg Gln Lys Ser 20 25 30 Ser Phe Gln Asp Leu Val Ser Asp Cys Asp Lys Gln Ala Gln Lys Met 35 40 45 Ile Val Gln Lys Ile Lys Asp His Phe Pro Asp Asp Ala Ile Leu Ala 50 55 60 Glu Glu Gly Leu Phe Glu Lys Gly Asp Arg Met Trp Ile Ile Asp Pro 65 70 75 80 Ile Asp Gly Thr Met Asn Tyr Val His Gly Leu Pro Ser Phe Ala Ile 85 90 95 Gly Ile Ala Tyr Val Glu Lys Glu Gln Val Ile Leu Gly Val Ala His 100 105 110 Asp Pro Val Leu Asn Glu Thr Tyr Tyr Ala Ile Lys Gly Gln Gly Ala 115 120 125 Tyr Lys Asn Gly Glu Arg Ile Asn Val Ser Glu Asn Ser Leu Leu Lys 130 135 140 Asp Ser Ile Gly Asn Thr Gly Phe Tyr Thr Asp Phe Thr Gly Ile Phe 145 150 155 160 Ile Ser Ala Ile Glu Lys Lys Val Arg Arg Val Arg Met Thr Gly Ser 165 170 175 Ala Ile Leu Ala Gly Ala Tyr Val Ala Cys Gly Arg Phe Asp Phe Phe 180 185 190 Ile Ala Lys Arg Ala Asn Ser Trp Asp Val Ala Pro Leu Phe Val Leu 195 200 205 Val Pro Glu Ala Gly Gly Ile Val Thr Asp Leu Ser Gly Asn Gln Ala 210 215 220 His Leu Asn Thr Gly Asn Phe Leu Phe Ser Asn Gly Leu Leu His Asp 225 230 235 240 Gln Val Leu Glu Val Ile Arg Glu Val Asn Lys Lys Val Arg Lys 245 250 255 <210> 190 <211> 201 <212> PRT <213> Thermotoga neapolitana <400> 190 Met Arg Leu Tyr Leu Ile Arg His Gly Glu Thr Ile Trp Asn Glu Lys 1 5 10 15 Gly Leu Trp Gln Gly Ile Ala Asp Val Pro Leu Asn Glu Lys Gly Lys 20 25 30 Asp Gln Ala Lys Lys Leu Ala Glu Arg Leu Lys Arg Val Asp Ala Ile 35 40 45 Tyr Ser Ser Pro Leu Lys Arg Cys Leu Glu Thr Ala Arg Glu Ile Ala 50 55 60 Glu Arg Phe Lys Lys Glu Val Ile Val Glu Glu Asp Leu Arg Glu Cys 65 70 75 80 Glu Ile Ser Leu Trp Asn Gly Leu Thr Val Glu Glu Ala Met Arg Glu 85 90 95 Tyr Pro Glu Glu Phe Lys Arg Trp Ser Thr Asp Pro Asn Phe Gly Thr 100 105 110 Lys Gly Leu Glu Ser Met Lys Ser Val Gln Asp Arg Met Val Lys Val 115 120 125 Met Met Lys Ile Val Ser Gln Glu Arg Leu Asn Gly Ser Glu Asp Val 130 135 140 Val Ile Val Ser His Ser Leu Ser Leu Arg Ser Phe Ile Cys Trp Val 145 150 155 160 Leu Gly Leu Pro Leu Arg Leu His Arg Asn Phe Lys Leu Asp Asn Ala 165 170 175 Ser Leu Ser Ile Val Asp Val Glu Ser Arg Pro Arg Leu Val Leu Leu 180 185 190 Asn Asp Thr Cys His Leu Glu Asp Thr 195 200 <210> 191 <211> 268 <212> PRT <213> Thermotoga neapolitana <400> 191 Met Tyr Arg Val Phe Val Phe Asp Leu Asp Gly Thr Leu Leu Asn Asp 1 5 10 15 Ser Leu Glu Ile Ser Glu Lys Asp Arg Lys Val Ile Glu Arg Leu Ser 20 25 30 Arg Asn Cys His Val Val Phe Ala Ser Gly Arg Met Leu Val Ser Thr 35 40 45 Leu Asn Val Glu Lys Arg Tyr Phe Lys Arg Thr Phe Pro Thr Ile Ala 50 55 60 Tyr Asn Gly Ala Met Val Tyr Leu Pro Glu Glu Gly Val Val Leu Asn 65 70 75 80 Glu Lys Ile Pro Pro Glu Val Ala Lys Asp Ile Ile Glu Tyr Ile Lys 85 90 95 Pro Leu Asn Val His Trp Gln Ala Tyr Ile Asp Asp Val Leu Tyr Ser 100 105 110 Glu Lys Asp Asn Glu Glu Ile Lys Ser Tyr Ala Arg His Ser Asn Val 115 120 125 Asp Tyr Arg Val Glu Pro Asn Leu Ser Glu Leu Val Ser Lys Met Gly 130 135 140 Thr Thr Lys Leu Leu Leu Ile Asp Thr Pro Glu Arg Leu Asp Glu Leu 145 150 155 160 Lys Glu Ile Leu Ser Glu Arg Phe Lys Asp Val Val Lys Val Phe Lys 165 170 175 Ser Phe Pro Thr Tyr Leu Glu Ile Val Pro Lys Asn Val Asp Lys Gly 180 185 190 Lys Ala Leu Lys Phe Leu Arg Gly Arg Met Asn Trp Lys Lys Glu Glu 195 200 205 Ile Val Val Phe Gly Asp Asn Glu Asn Asp Leu Phe Met Phe Glu Glu 210 215 220 Ala Gly Leu Arg Val Ala Met Gly Asn Ala Ile Asp Lys Val Lys Glu 225 230 235 240 Ala Ala Asp Val Val Thr Leu Thr Asn Asn Asp Ser Gly Val Ser Tyr 245 250 255 Val Leu Glu Arg Ile Ser Thr Asp Cys Leu Asp Glu 260 265 <210> 192 <211> 201 <212> PRT <213> Thermotoga neapolitana <400> 192 Met Ile Arg Asn Ile Val Phe Asp Leu Gly Gly Val Leu Ile Asp Trp 1 5 10 15 Arg Pro Cys Glu Tyr Leu Val Glu Ser Phe Pro Glu Asp Val Ala Lys 20 25 30 Val Leu Glu Arg Glu Ile Phe Lys His Glu Asp Trp Lys Lys Met Asp 35 40 45 Arg Gly Thr Leu Pro Glu Asn Asp Leu Trp Glu Lys Lys Lys Lys Glu 50 55 60 Leu Ser Glu Tyr Arg Glu Tyr Val Glu Lys Leu Glu Arg Glu Val Pro 65 70 75 80 Lys Leu Leu Lys Pro Ile Glu Glu Asn Val Lys Leu Leu Ser Ile Leu 85 90 95 Lys Glu Lys Asn Phe Lys Leu Tyr Val Leu Ser Asn Tyr Gly Lys Ile 100 105 110 Tyr Phe Glu Met Val Arg Arg Arg Tyr Arg Phe Phe Asp Phe Phe Asp 115 120 125 Gly Met Val Ile Ser Ser His Val Gly Phe Ile Lys Pro Glu Lys Glu 130 135 140 Ile Tyr Leu Glu Leu Ile Arg Lys Tyr Lys Ile Thr Pro Lys Glu Ser 145 150 155 160 Leu Phe Ile Asp Asp Met Glu Glu Asn Val Lys Ala Ala Glu Glu Leu 165 170 175 Gly Phe Asn Thr Ile His Leu Pro Glu Pro Ser Arg Leu Lys Glu Leu 180 185 190 Leu Phe Glu Thr Leu Lys Ile Gly Arg 195 200 <210> 193 <211> 564 <212> PRT <213> Thermotoga neapolitana <400> 193 Met Leu Leu Arg Ile Ile Pro Ser Asp Arg Ile Ser Ser Thr Glu Val 1 5 10 15 Lys Thr Leu Glu Arg Val Tyr Val Val Gly His Arg Asn Pro Asp Thr 20 25 30 Asp Ser Val Cys Ser Ala Ile Gly Tyr Ala His Tyr Lys Ala Glu Val 35 40 45 Glu Lys Lys Lys Gln Phe Ile Pro Ala Arg Cys Gly Glu Leu Thr Asn 50 55 60 Glu Ala Leu Phe Val Leu Asp Tyr Phe Lys Val Arg Pro Pro Ile Phe 65 70 75 80 Leu Glu Thr Leu Glu Pro Thr Val Glu Asp Leu Glu Leu Lys Asp Pro 85 90 95 Ile Phe Val Ser Pro Asp Thr Pro Val Tyr Asp Val Ala Met Leu Met 100 105 110 Glu Gly Lys Gly Ile Lys Asn Val Pro Val Val Ser Arg Gly Lys Met 115 120 125 Ile Gly Val Val Thr Glu Ser Asn Leu Ala Arg Val Tyr Val Arg Arg 130 135 140 Leu Lys Ile Glu Pro Leu Val Ile His Pro Val Pro Leu Glu Gln Leu 145 150 155 160 Val Arg Val Leu Lys Ala Glu Val Val Cys Asp His Leu Lys Glu Lys 165 170 175 Thr Ile Ser Gly Lys Val His Ile Ala Val Asp Ala Leu His Val Leu 180 185 190 Leu Gly Lys Ile Glu Ile Gly Asp Val Val Ile Val Gly Asp Asn Glu 195 200 205 Pro Ser Gln Ile Ala Leu Leu Glu Lys Gly Ala Lys Leu Met Ile Ile 210 215 220 Val Asn Asn Ala Pro Val Ser Ser Arg Val Leu Glu Ile Ala Lys Glu 225 230 235 240 Lys Gly Ala Ala Val Leu Arg Val Lys Phe Asp Ala Phe Gly Ala Ala 245 250 255 Lys Leu Ile Asn Leu Ala Leu Pro Val Thr Leu Val Met Ser Lys Lys 260 265 270 Phe Pro Thr Val Thr Lys Arg Asp Thr Leu Glu Asp Val Lys Asn Ile 275 280 285 Val Phe Thr Ser Lys Leu Arg Ala Ala Phe Val Glu Asp Glu Lys Gly 290 295 300 Lys Leu Leu Gly Val Ile Thr Arg Thr Asp Leu Met Lys Asp Val Arg 305 310 315 320 Lys Lys Val Ile Leu Val Asp His Asn Glu Ile Thr Gln Ala Pro Glu 325 330 335 Gly Val Glu Lys Ala Glu Ile Leu Glu Ile Ile Asp His His Arg Leu 340 345 350 Gly Gly Leu Ser Thr Leu Asn Pro Val Phe Phe Tyr Asn Glu Pro Val 355 360 365 Gly Ser Thr Ser Thr Ile Val Thr Glu Phe Phe Leu Arg Asp Gly Val 370 375 380 Lys Met Glu Arg Glu Ile Ala Gly Val Leu Leu Ala Gly Ile Val Ser 385 390 395 400 Asp Thr Leu Phe Phe Lys Leu Ser Thr Thr Thr Glu Lys Asp Arg Ala 405 410 415 Met Ala Gly Phe Leu Ala Arg Ile Thr Lys Leu Asp Leu Glu Lys Phe 420 425 430 Ala Lys Lys Val Leu Lys Glu Gly Met Lys Ile Pro Glu Asn Val Asp 435 440 445 Pro Leu Asp Leu Leu Lys Arg Asp Val Lys Val Tyr Glu Met Gly Glu 450 455 460 Glu Ser Phe Ala Val Ser Gln Ile Met Thr Ser Asp Phe Ser Val Leu 465 470 475 480 Leu Lys Glu Lys Glu Lys Phe Ala Thr Ala Leu Lys Ser Leu Lys Gly 485 490 495 Glu Leu Gly Val Thr His Cys Phe Val Leu Phe Thr Asn Pro Ile Glu 500 505 510 Glu Ala Ser Leu Val Met Val Glu Gly Asp Gln Arg Ile Leu Glu Lys 515 520 525 Ala Phe Asp Gly Ala Glu Lys Lys Asp Gly Leu Phe Leu Leu Lys Gly 530 535 540 Val Met Ser Arg Lys Lys Asp Phe Val Pro Arg Ile Gly Asp Ile Leu 545 550 555 560 Arg Arg Glu Arg <210> 194 <211> 136 <212> PRT <213> Thermotoga neapolitana <400> 194 Met Asp Thr Phe Leu Tyr His Ile Leu Ala Ile Asp Val Lys Gly Tyr 1 5 10 15 Val Asp Pro Ser Leu Pro Val Glu Glu Ile Val Lys Arg Leu Lys Glu 20 25 30 Gln Asn Ala Leu Val Ile Ala Ala His Pro Asp Arg Lys Lys Gln Asp 35 40 45 Glu Glu His Leu Ser Trp Phe Leu Trp Val Asn Met Glu Arg Phe Lys 50 55 60 Asp Met Phe Asp Ala Trp Glu Val Ala Asn Arg Asp Asp Leu Phe Asn 65 70 75 80 Ser Ile Gly Val Lys Lys Tyr Arg Tyr Val Ala Asn Ser Asp Phe His 85 90 95 Glu Val Trp His Val Tyr Ser Trp Lys Thr Leu Ile Arg Ser Glu Arg 100 105 110 Asn Val Glu Ala Ile Lys Glu Ala Ile Arg Lys Asn Thr Asp Val Ala 115 120 125 Ile Tyr Leu Met Arg Glu Lys Thr 130 135 <210> 195 <211> 443 <212> PRT <213> Thermotoga neapolitana <400> 195 Met Asn Glu Arg Asp Ile Cys Arg Arg Ile Ser Glu Ile Leu Gly Ile 1 5 10 15 Asp Tyr Asp Cys Thr Arg Gly Ile Asp Glu Leu Leu Lys Ile Leu Glu 20 25 30 Tyr Glu Ile Asn Glu Tyr Lys Lys Ser Leu Glu Glu Gln Thr Ile Phe 35 40 45 Met Glu Ala Gln Leu Glu Glu Leu Ser Arg Ser Tyr Glu Glu Ile Ser 50 55 60 Thr Leu Leu Glu Ile Ser Glu Ile Phe Gly Gly Phe Glu Phe Pro Met 65 70 75 80 Asn Leu Arg Glu Lys Leu Glu Lys Val Ile Pro Leu Leu Lys Lys Val 85 90 95 Val Lys Phe Lys Asp Tyr Ile Val Arg Val Glu Asp Leu Ser Ile Gly 100 105 110 Asn Met Ser His Glu Glu Val Glu Arg Glu Ile Glu Glu Arg Glu Lys 115 120 125 Thr Val Leu Ile Glu Pro Gly Glu Ser Lys Lys Phe Ser Asn Leu Leu 130 135 140 Phe Val Pro Ile Ile Gly Asn Arg Tyr Tyr Gly Tyr Met Cys Phe Ser 145 150 155 160 Gly Lys Glu Glu Gly Ile Val Phe Thr Ala Ser Asp Arg Arg Ile Thr 165 170 175 Glu Val Thr Ser Arg Tyr Ile Ala Asn Ala Leu Asp Arg Ile Asp Phe 180 185 190 Leu Gln Lys Glu Ile Glu Arg Gln Arg Leu Glu Glu Gln Met Glu Ile 195 200 205 Ala Arg Arg Ile Gln Ser Glu Leu Leu Pro Arg Glu Leu Pro Arg Thr 210 215 220 Gly Phe Ile Asp Ile Thr Ala Cys Ser Arg Pro Ala Val Gln Val Gly 225 230 235 240 Gly Asp Tyr Tyr Asp Val Phe Ala Ser Asp Gly Lys Val Leu Ala Val 245 250 255 Met Gly Asp Val Ala Gly Lys Ser Val Pro Ala Ala Leu Leu Met Ser 260 265 270 Ala Val Arg Ser Tyr Leu Lys Val Leu Ser Thr Ser His Ser Asp Leu 275 280 285 Glu Asp Leu Val Asn His Leu Asn Ser Ile Leu Cys Glu Asp Leu Ser 290 295 300 Asn Asp Arg Phe Val Thr Met Val Phe Leu Glu Ile Phe Gln Asn Gly 305 310 315 320 Thr Leu Asn Leu Val Asn Ala Gly His Asn Pro Val Tyr Phe Leu His 325 330 335 Asp Gly Glu Ile Val Lys Leu Glu Ala Ser Ala Ile Pro Ile Gly Ile 340 345 350 Ala Glu Trp Asn Tyr Arg Arg His Thr Ile Gln Leu Lys Pro Asn Thr 355 360 365 Phe Ile Val Val Tyr Thr Asp Gly Ile Thr Glu Ala Arg Asn Thr Leu 370 375 380 Gly Glu Glu Tyr Gly Tyr Glu Arg Leu Glu Glu Thr Leu Arg Glu Tyr 385 390 395 400 Asn Gly Asp Ser Ala Glu Asp Leu Leu Ser Lys Leu Lys Lys Ser Val 405 410 415 Glu Glu Phe Ser Lys Asp Val Pro Gln His Asp Asp Met Thr Ile Met 420 425 430 Val Leu Lys Tyr Lys Gly Gln Gln Glu Val Glu 435 440 <210> 196 <211> 266 <212> PRT <213> Thermotoga neapolitana <400> 196 Met Ile Ala Leu Leu Asp Met Gly Ser Asn Ser Phe Ile Leu Leu Ile 1 5 10 15 Val Ser Glu Glu Gly Glu Val Ile Leu Glu Glu Val His Glu Val Gly 20 25 30 Ile Ala Ser Gly Asn Leu Gln Arg Ala Lys Glu Val Phe Arg Glu Cys 35 40 45 Val Arg Lys Ser Glu Glu Leu Gly Ala Asp Leu His Ile Phe Gly Thr 50 55 60 Ala Phe Phe Arg Lys Asn Pro Asp Val Phe Tyr Glu Ile Thr Gly Gly 65 70 75 80 Arg Gly Lys Ile Leu Ser Glu Glu Glu Glu Ala Arg Tyr Ser Tyr Ile 85 90 95 Ser Val Val Arg Asp Phe Gly Lys Glu Asp Ile Leu Val Ala Asp Leu 100 105 110 Gly Gly Gly Ser Leu Glu Leu Ala Trp Lys Asp Gly Tyr Thr Ser Leu 115 120 125 Glu Leu Gly Thr His Ile Leu Asn Arg Ile Phe Ser Leu Thr Leu Pro 130 135 140 Phe Arg Lys Ser Val Asp Asp Val Val Glu Tyr Val Met Asp Lys Leu 145 150 155 160 Pro Asp Leu Ala Lys Ser Asp Leu Phe Gly Val Gly Gly Ser Phe Val 165 170 175 Ala Leu Ala Ala Leu Met Lys Gly Lys Trp Asp Leu Lys Ser Ile His 180 185 190 Gly Ser Thr Leu Glu Ile Glu Arg Val Gln Lys Ile Val Asp Gln Ile 195 200 205 Arg Lys Met Ser Phe Glu Asp Ile Arg Lys Leu Lys Ile Leu Pro Glu 210 215 220 Gly Arg Glu Lys Thr Ile Leu Ala Gly Gly Ile Val Thr Ile Ala Leu 225 230 235 240 Leu Lys Lys Tyr Ser Pro Lys Met Thr Val Ser Thr Lys Gly Tyr Arg 245 250 255 Tyr Gly Ile Ala Trp Glu Ile Met Val Gln 260 265 <210> 197 <211> 208 <212> PRT <213> Thermotoga neapolitana <400> 197 Met Glu Leu Ile Lys Leu Lys Lys Gly Gly Val Met Val Gly Thr Phe 1 5 10 15 Arg Ile Tyr Val Ala Thr Thr Asn Pro His Lys Val Glu Glu Ile Lys 20 25 30 Glu Ile Ala Pro Glu Trp Ala Glu Ile Leu Pro Ser Pro Glu Lys Ile 35 40 45 Glu Val Ile Glu Asp Gly Glu Thr Phe Leu Glu Asn Ser Val Lys Lys 50 55 60 Ala Ile Val Tyr Gly Lys Lys Leu Lys Ser Pro Val Ile Ala Asp Asp 65 70 75 80 Ser Gly Leu Val Ile Tyr Ser Leu Gly Gly Phe Pro Gly Val Met Ser 85 90 95 Ala Arg Phe Met Glu Glu Tyr Thr Tyr Glu Glu Lys Met Lys Thr Ile 100 105 110 Leu Lys Met Leu Glu Gly Lys Asp Arg Lys Ala Ala Phe Val Cys Ser 115 120 125 Ala Thr Phe Phe Asp Phe Gln Arg Asn Leu Leu Val Ser Val Glu Asp 130 135 140 Arg Val Glu Gly Tyr Ile Ala Glu Glu Ile Arg Gly Thr Gly Gly Phe 145 150 155 160 Gly Tyr Asp Pro Phe Phe Val Pro Glu Gly Tyr Asp Arg Thr Phe Gly 165 170 175 Glu Met Pro Glu Leu Lys Lys Lys Leu Ser His Arg Ser Arg Ala Phe 180 185 190 Arg Lys Leu Phe Ser Ile Leu Ser Lys Ile Ser Glu Ser Glu Ser Leu 195 200 205 <210> 198 <211> 436 <212> PRT <213> Thermotoga neapolitana <400> 198 Met Ile Gln Val Arg Arg Leu Ile Val Leu Val Leu Ala Leu Leu Leu 1 5 10 15 Val Ala Phe Ser Phe Ser Gln Val Lys Asn Ile Ile Tyr Phe Ile Gly 20 25 30 Asp Gly Met Gly Leu Ser Gln Ala Tyr Leu Thr Ser Leu Ile Glu Gly 35 40 45 Arg Pro Leu Ser Phe Met Lys Thr Pro Tyr Thr Gly Leu Val Lys Thr 50 55 60 His Ser Val Asp Ser Trp Val Thr Asp Ser Ala Ala Ala Gly Thr Ala 65 70 75 80 Leu Ala Ser Gly Phe Lys Thr Lys Asn Gly Met Ile Asn Val Leu Pro 85 90 95 Asp Gly Thr Arg Val Pro Thr Ile Phe Glu Ile Ala Lys Ala Tyr Gly 100 105 110 Ala Lys Thr Gly Ile Val Val Thr Cys Arg Val Thr His Ala Thr Pro 115 120 125 Ala Ala Phe Tyr Ala His Val Lys Ser Arg Ser Glu Glu Asn Glu Ile 130 135 140 Ala Arg Gln Leu Val Glu Ser Glu Thr Val Asp Leu Val Met Gly Gly 145 150 155 160 Gly Trp Ala Asn Phe Leu Pro Glu Ser Leu Gly Gly Lys Arg Ser Asp 165 170 175 Gly Leu Asn Leu Ile Glu Met Ala Lys Lys Lys Gly Tyr Val Tyr Val 180 185 190 Thr Lys Lys Glu Asp Leu Met Lys Leu Pro Glu Asn Thr Glu Lys Val 195 200 205 Leu Ala Leu Phe Ala Pro Ser His Leu Asp Pro Ala Ser Ser Arg Glu 210 215 220 Glu Gln Pro Met Leu Tyr Glu Met Val Lys Lys Ala Leu Glu Ile Leu 225 230 235 240 Ser Asn Asp Glu Glu Pro Phe Phe Leu Met Val Glu Gly Ser Gln Ile 245 250 255 Asp Trp Glu Ala His Asp Asn Asp Ile Tyr Gly Val Trp Lys Glu Val 260 265 270 Val Glu Phe Asp Arg Ala Val Gln Val Ala Leu Asp Phe Ala Leu Lys 275 280 285 Arg Gly Asp Thr Leu Val Ile Val Thr Ala Asp His Glu Thr Gly Gly 290 295 300 Leu Ala Leu Ser Ser Gly Asp Tyr Arg Val Asp Val Asp Arg Ile Arg 305 310 315 320 Lys Phe Ser Lys Thr Thr Asp Trp Ile Leu Ala Asn Tyr Ser Leu Lys 325 330 335 Asp Arg Glu Ser Phe Lys Lys Ala Ile Glu Lys Tyr Phe Gly Leu Thr 340 345 350 Leu Ser Asp Ser Asp Leu Asp Arg Ile Thr Ser Ser Asn Asn Ser Lys 355 360 365 Val Glu Leu Gly Arg Val Leu Ser Glu Lys Val Asn Val Gly Trp Thr 370 375 380 Thr Thr Ser His Ser Gly Val Pro Val Pro Ile Tyr Ala Phe Gly Pro 385 390 395 400 Gly Ala Glu Asn Phe Thr Gly Phe Leu Asp Asn Thr Asp Ile Pro Arg 405 410 415 Ile Met Met Lys Leu Ala Gly Tyr Ser Leu Gln Tyr Pro Leu Val Lys 420 425 430 Glu Pro Val Lys 435 <210> 199 <211> 174 <212> PRT <213> Thermotoga neapolitana <400> 199 Met Lys Ile Leu Ile Thr Gly Arg Pro Gly Val Gly Lys Thr Thr Leu 1 5 10 15 Ile Lys Lys Ile Ser Ser Leu Leu Gln Asn Ala Gly Gly Phe Tyr Thr 20 25 30 Glu Glu Met Arg Glu Lys Gly Lys Arg Val Gly Phe Lys Ile Val Thr 35 40 45 Leu Asp Gly Arg Glu Gly Ile Leu Ala Lys Val Gly Phe Pro Ser Gln 50 55 60 His Arg Val Gly Lys Tyr Gly Val Asn Leu Lys Asp Leu Glu Glu Leu 65 70 75 80 Gly Val Asp Ser Val Glu Arg Ala Leu Glu Glu Lys Ser Val Val Ile 85 90 95 Ile Asp Glu Ile Gly Lys Met Glu Leu Leu Ser Glu Arg Phe Lys Arg 100 105 110 Val Val Glu Lys Ala Phe Asn Ser Glu Lys Asp Leu Ile Ala Thr Ile 115 120 125 Lys Lys Ser Ser Asp Pro Phe Val Glu Lys Ile Lys Asn Lys Lys Asp 130 135 140 Val Ile Ile Phe Glu Leu Asn Glu Arg Asn Arg Asp Leu Leu Leu Lys 145 150 155 160 Arg Ile Leu Asp Met Leu Lys Ser Asn Arg Gly Val Gly Glu 165 170 <210> 200 <211> 508 <212> PRT <213> Thermotoga neapolitana <400> 200 Met Arg Arg Tyr Leu Val Leu Phe Leu Val Leu Leu Leu Ser Val Phe 1 5 10 15 Leu Leu Gly Val Arg Leu Thr Ile Leu His Val Asn Asp Thr His Gly 20 25 30 His Ala Trp Thr Phe Asp Glu Tyr Gln Asn Pro Gly Ile Gly Gly Leu 35 40 45 Ala Ala Ile Ala Thr Ile Val Glu Glu Val Lys Arg Glu Val Glu Glu 50 55 60 Gln Gly Gly Tyr Val Ile Phe Leu His Ala Gly Asp Leu Asn Thr Gly 65 70 75 80 Val Pro Glu Ser Asp Leu Gln Asp Ala Ile Pro Asp Ile Val Gly Tyr 85 90 95 Asn Met Ile Gly Leu Ser Ala Met Ala Val Gly Asn His Glu Phe Asp 100 105 110 Asn Pro Arg Asp Val Leu Leu Lys Gln Met Lys Phe Ala Asp Phe Pro 115 120 125 Phe Leu Ser Ala Asn Ile Leu Lys Glu Asn Gly Glu Pro Phe Phe Thr 130 135 140 Pro Tyr Ile Leu Lys Asp Phe Gly Lys Leu Lys Val Ala Ile Ile Gly 145 150 155 160 Phe Thr Thr Glu Glu Thr Thr Ile Leu Glu Pro Leu Tyr Leu Glu Asp 165 170 175 Leu Lys Phe Glu Asn Ala Leu Lys Val Ala Gln Lys Ile Ala Pro Glu 180 185 190 Leu Lys Asn Gln Ala Asp Val Val Ile Ala Leu Ala His Leu Asp Trp 195 200 205 Gly Glu Pro Lys Lys Glu Asn Ile Thr Thr Thr His Gln Leu Ala Lys 210 215 220 Val Asp Gly Ile Asp Val Val Val Ala Gly His Ser His Val Leu Gly 225 230 235 240 Ser Glu Val Val Asp Glu Lys Ile Ile Val Ser Ala Gly Glu Tyr Gly 245 250 255 Lys Tyr Val Gly Arg Leu Asp Leu Asp Ile Glu Asp Gly Lys Ile Val 260 265 270 Ala Trp His Trp Gln Ala Ile Pro Val Asn Leu Lys Ala Tyr Arg Asp 275 280 285 Gly Lys Tyr Glu Tyr Val Gly Lys Pro Tyr Leu Glu Asn Arg Tyr Val 290 295 300 Ala Lys Ala Leu Glu Tyr Phe Arg Lys Val Gly Asn Glu Lys Leu Asp 305 310 315 320 Thr Val Ile Gly Glu Thr Lys Ile Phe Leu Asp Gly Glu Arg Glu His 325 330 335 Val Arg Ser Arg Asn Thr Asn Leu Thr Asn Leu Ile Thr Asp Ala Met 340 345 350 Arg Trp Lys Val Lys Ala Asp Ile Ala Leu Thr Asn Gly Glu Gly Ile 355 360 365 Arg Ala Ser Ile Lys Pro Gly Lys Ile Thr Val Arg Asp Ile Leu Thr 370 375 380 Val Leu Pro Phe Gly Asn Thr Leu Tyr Val Leu Glu Leu Thr Gly Glu 385 390 395 400 Gln Ile Met Lys Val Leu Glu Tyr Ser Ala Thr Ile Pro Glu Gly Lys 405 410 415 Gly Ala Phe Leu Gln Val Ser Gly Leu Thr Trp Lys Ser Lys Asp Gly 420 425 430 Lys Val Val Glu Val Leu Val Asn Gly Glu Pro Leu Asp Pro Lys Lys 435 440 445 Thr Tyr Arg Val Val Thr Asn Asn Tyr Met Ala Gly Gly Gly Asp Gly 450 455 460 Tyr Thr Met Leu Lys Glu Trp Gly Gly Tyr Asp Thr Gly Phe Leu Met 465 470 475 480 Ser Asp Ala Val Ile Glu Tyr Ile Gln Asn Val Leu Asn Gly Val Ile 485 490 495 Glu Glu Tyr Asp Asn Ser Gln Arg Tyr Val Arg Glu 500 505 <210> 201 <211> 154 <212> PRT <213> Thermotoga neapolitana <400> 201 Met Asp Ala Lys Ile Val Asn Ala Leu Ile Gly Ser Val Tyr Glu Thr 1 5 10 15 Ile Lys Ser Val Leu Arg Val Glu Pro Lys Leu Gly Lys Pro Ser Ala 20 25 30 Val Ser His Ile Glu Ile Pro His Ser Val Val Thr Val Ile Gly Val 35 40 45 Thr Gly Ser Ile Glu Gly Ser Leu Ile Tyr Ser Phe Ser Ser Glu Thr 50 55 60 Ala Leu Lys Val Val Ser Ala Met Met Gly Met Glu Tyr Gly Gln Leu 65 70 75 80 Asp Glu Leu Ala Met Ser Ala Ile Gly Glu Leu Gly Asn Met Thr Ala 85 90 95 Gly Lys Leu Ala Met Lys Leu Glu Thr Leu Gly Lys His Val Asp Ile 100 105 110 Thr Pro Pro Thr Val Val Ser Gly Lys Asp Leu Lys Ile Lys Ser Phe 115 120 125 Gly Thr Val Leu Lys Leu Pro Ile Ser Val Phe Ser Asn Glu Asp Phe 130 135 140 Asp Leu His Leu Ser Val Lys Ser Gly Gly 145 150 <210> 202 <211> 333 <212> PRT <213> Thermotoga neapolitana <400> 202 Met Lys Glu Ile Val Asp Ala Ile Ser Gln Tyr Asn Arg Ile Leu Val 1 5 10 15 Val Gly His Ile Met Pro Asp Gly Asp Cys Val Ser Ser Val Leu Ser 20 25 30 Leu Thr Leu Gly Leu Glu Arg Ile Gly Lys Glu Val Arg Ala Ala Ile 35 40 45 Asp Tyr Arg Val Pro Tyr Val Phe Glu Asn Phe Pro His Ile Glu Arg 50 55 60 Ile Glu Gln Asn Val Asp Phe Glu Pro Glu Leu Val Ile Val Val Asp 65 70 75 80 Ala Ser Ser Pro Asp Arg Ile Gly Arg Phe Gln Asp Leu Leu Lys Glu 85 90 95 Val Pro Ser Ala Val Ile Asp His His Ser Thr Asn Val Leu Phe Gly 100 105 110 Lys Trp Asn Trp Val Asp Pro Ser Phe Ala Ala Thr Ala Gln Met Val 115 120 125 Phe Arg Leu Asn Arg Lys Leu Gly Val Glu Tyr Asp Pro Val Leu Ala 130 135 140 Thr Leu Asn Tyr Leu Gly Ile Ala Thr Asp Thr Gly Phe Phe Arg His 145 150 155 160 Ser Asn Thr Asp Ala Arg Val Phe Glu Asp Ala Tyr Glu Leu Val Lys 165 170 175 Ile Gly Ala Asp Ala His Phe Val Ala Lys Glu Ile Leu Glu Asn Lys 180 185 190 Arg Phe Glu Gln Phe Lys Leu Phe Ala Glu Val Leu Glu Arg Leu Gln 195 200 205 Leu Leu Glu Asn Gly Arg Ile Ala Tyr Ser Tyr Ile Asp Tyr Asp Thr 210 215 220 Tyr Leu Arg His Asn Cys Thr Asp Glu Asp Ser Ala Gly Phe Val Gly 225 230 235 240 Glu Leu Arg Ser Ile Arg Gly Val Glu Val Ala Val Leu Phe Met Glu 245 250 255 Phe Pro Lys Gly Lys Ile His Val Ser Met Arg Ser Lys Asp Trp Phe 260 265 270 Asn Val Asn Glu Val Ala Phe Glu Leu Gly Gly Gly Gly His Pro Arg 275 280 285 Ala Ala Gly Val Thr Phe Glu Gly Glu Lys Leu Glu Asn Val Ile Ser 290 295 300 Arg Val Ile Asp His Leu Leu Gly Arg Phe Lys Glu Gly Val Ser Arg 305 310 315 320 Glu Gly Glu Lys Thr Ser Gln Arg Ser Val Leu Gly Arg 325 330 <210> 203 <211> 295 <212> PRT <213> Thermotoga neapolitana <400> 203 Met Ile Leu Arg Arg Arg Gly Ile Val Leu Ser Phe His Ser Asn Met 1 5 10 15 Val Thr Val Glu Asp Glu Glu Thr Gly Glu Arg Leu Leu Cys Lys Leu 20 25 30 Arg Gly Lys Phe Arg Leu Gln Asn Leu Lys Ile Tyr Val Gly Asp Arg 35 40 45 Val Glu Tyr Thr Pro Asp Gly Thr Gly Ser Gly Val Ile Glu Asn Val 50 55 60 Leu His Arg Lys Asn Leu Leu Val Lys Pro His Val Ala Asn Val Asp 65 70 75 80 Gln Ala Ile Leu Val Val Thr Val Lys Met Pro Glu Thr Ser Thr Tyr 85 90 95 Ile Ile Asp Lys Phe Leu Val Leu Thr Glu Lys Asn Glu Leu Glu Thr 100 105 110 Val Leu Val Ile Asn Lys Met Asp Ile Tyr Asp Glu Glu Asp Leu Glu 115 120 125 Lys Val Lys Glu Leu Glu Arg Ile Tyr Ser Lys Leu Tyr Pro Ile Val 130 135 140 Lys Thr Ser Ala Lys Thr Gly Met Gly Ile Glu Glu Leu Lys Lys Tyr 145 150 155 160 Leu Lys Gly Lys Ile Ser Thr Met Ala Gly Leu Ser Gly Val Gly Lys 165 170 175 Ser Ser Leu Leu Asn Ala Ile Asn Pro Gly Leu Lys Leu Arg Val Ser 180 185 190 Glu Val Ser Gln Lys Leu Gln Arg Gly Arg His Thr Thr Thr Ser Ala 195 200 205 Gln Leu Leu Arg Phe Asp Phe Gly Gly Tyr Val Val Asp Thr Pro Gly 210 215 220 Phe Ala Asn Leu Glu Ile Gly Asp Ile Glu Pro Glu Glu Leu Lys Tyr 225 230 235 240 Tyr Phe Lys Glu Phe Asn Glu Lys Gln Cys Phe Phe Ser Asp Cys Asn 245 250 255 His Ile Asp Glu Pro Glu Cys Gly Val Lys Glu Ala Val Glu Asn Gly 260 265 270 Glu Ile Ala Glu Ser Arg Tyr Glu Asn Tyr Val Lys Met Phe His Glu 275 280 285 Leu Leu Gly Arg Gly Lys Ser 290 295 <210> 204 <211> 528 <212> PRT <213> Thermotoga neapolitana <400> 204 Met Lys Lys Ile Thr Gly Arg Phe Leu Lys Thr Ile Ile Lys Lys Ala 1 5 10 15 Thr Glu Asn Leu Leu Lys His Lys Asp Glu Ile Asn Ala Leu Asn Val 20 25 30 Phe Pro Val Pro Asp Gly Asp Thr Gly Ser Asn Met Cys Ser Thr Met 35 40 45 Leu Glu Ala Cys Lys Tyr Ile Asp Asn Leu Lys Ser Asp Asp Leu Ser 50 55 60 Glu Val Trp Lys Ala Ile Lys Glu Gly Ala Leu Met Gly Ala Arg Gly 65 70 75 80 Asn Ser Gly Val Ile Leu Ser Gln Ile Leu Arg Gly Leu Ala Asp Ala 85 90 95 Ser Pro Glu Asn Tyr Ile Thr Pro Gly Asp Phe Ile Lys Met Ile Ser 100 105 110 Asn Ala Arg Lys Val Ala Tyr Ser Ala Val Met Arg Pro Val Glu Gly 115 120 125 Thr Met Leu Thr Val Val Arg Glu Leu Asp Glu Lys Leu Lys Gly Arg 130 135 140 Ser Phe Glu Thr Phe Glu Glu Leu Phe Asp Gln Ile Val Glu Met Ile 145 150 155 160 Lys Asp Thr Val Asn Arg Thr Pro Ser Met Leu Ser Lys Leu Arg Glu 165 170 175 Ala Gly Val Val Asp Ala Gly Ala Lys Gly Leu Tyr Tyr Leu Phe Glu 180 185 190 Gly Met Arg Asp Ala Ile Lys Gly Asp Ile Glu Val Asn Leu Glu Gln 195 200 205 Val Glu Gln Ala Ser Val Glu Asp Leu Lys Arg Met Ala Leu Glu Glu 210 215 220 Ile Thr Asn Gln Tyr Cys Thr Glu Val Ala Val Arg Arg Lys Arg Val 225 230 235 240 Phe Glu Lys Ser Glu Leu Glu Ser Phe Leu Asn Glu Ile Gly Asp Ser 245 250 255 Val Val Leu Val Glu Gln Asp Asp Ile Phe Arg Leu His Val His Thr 260 265 270 Asn His Pro Gly Gln Val Leu Glu Lys Val Leu Asp Phe Gly Glu Ile 275 280 285 Ile Lys Val Lys Val Asp Asn Met Lys Leu Gln His Glu His Ile Ile 290 295 300 Ser Ala Gln Val Glu Lys Glu Ile Gly Val Val Ala Val Ser Pro Gly 305 310 315 320 Lys Gly Ile Ser Glu Ile Leu Lys Ser Leu Gly Val Asp Glu Ile Val 325 330 335 Pro Gly Gly Gln Thr Met Asn Pro Ser Phe Ala Asp Leu Lys Ala Ala 340 345 350 Val Asp Lys Thr His Ala Lys Val Val Phe Leu Phe Pro Asn Asn Ala 355 360 365 Asn Val Leu Leu Thr Ala Lys Gln Val Ala Glu Ser Val Asp Asp Lys 370 375 380 Arg Val Ile Val Val Gln Thr Ser His Val Gln Glu Cys Val Ala Ala 385 390 395 400 Met Val Glu Tyr Asp Pro Asp Glu Asp Pro Glu Glu Leu Lys Arg Arg 405 410 415 Phe Glu Glu Ala Ile Asn Gln Cys Val Pro Ile Ser Ile Thr Arg Ala 420 425 430 Val Arg Asp Ser Arg Tyr Gly Lys Arg Arg Ile Arg Lys Gly Glu Tyr 435 440 445 Leu Val Phe Val Arg Lys Glu Leu Leu Ala His Gly Phe Asn Leu Val 450 455 460 Lys Ala Leu Lys Asp Val Leu Glu Lys Glu Asn Ala Arg Glu Lys Glu 465 470 475 480 Ile Leu Thr Val Phe Leu Gly Asp Asn Tyr Arg Lys Ala Glu Leu Glu 485 490 495 Lys Ile Gln Asn Leu Ile Gly Glu Glu Phe Pro Asn Leu Asp Leu Glu 500 505 510 Ile His Glu Gly Gly Gln Pro His Tyr Pro Phe Leu Met Leu Leu Gln 515 520 525 <210> 205 <211> 263 <212> PRT <213> Thermotoga neapolitana <400> 205 Met Glu Gly Gly Ile Glu Leu Asp Arg Leu Asp Phe Ser Ile Lys Leu 1 5 10 15 Leu Arg Arg Val Gly His Phe Leu Met Leu His Trp Gly Lys Val Asp 20 25 30 Ser Val Glu Lys Lys Thr Gly Phe Lys Asp Ile Val Thr Glu Ile Asp 35 40 45 Lys Lys Ala Gln Glu Met Ile Val Glu Glu Ile Arg Lys Val Phe Pro 50 55 60 Asp Glu Asn Ile Ile Ala Glu Glu Gly Ile Ser Glu Asn Gly Lys Lys 65 70 75 80 Leu Trp Ile Ile Asp Pro Ile Asp Gly Thr Ile Asn Phe Val His Gly 85 90 95 Leu Pro Asn Phe Ser Ile Ser Ile Ala Tyr Val Glu Asn Gly Glu Val 100 105 110 Lys Met Gly Val Val His Ala Pro Ala Leu Asn Glu Thr Leu Tyr Ala 115 120 125 Glu Glu Asn Gly Gly Ala Phe Leu Asn Gly Glu Arg Ile Arg Val Ser 130 135 140 Gly Asn Thr Ser Leu Glu Glu Cys Val Gly Ser Thr Gly Ser Tyr Val 145 150 155 160 Asp Phe Thr Gly Lys Phe Ile Glu Lys Met Glu Lys Lys Thr Arg Arg 165 170 175 Val Arg Ile Leu Gly Ser Ala Ala Leu Asn Ala Cys Tyr Val Gly Ala 180 185 190 Gly Arg Val Asp Phe Phe Val Thr Trp Arg Ile Asn Pro Trp Asp Ile 195 200 205 Ala Ala Gly Leu Ile Val Val Lys Glu Ala Gly Gly Thr Val Thr Asp 210 215 220 Phe Ala Gly Lys Glu Ala Asn Val Phe Ser Lys Asn Phe Val Phe Ser 225 230 235 240 Asn Gly Leu Val His Glu Glu Val Leu Glu Val Val Asn Glu Val Leu 245 250 255 Lys Glu Ile Gly Glu Gly Lys 260 <210> 206 <211> 90 <212> PRT <213> Thermotoga neapolitana <400> 206 Met Lys Ala Leu Lys Ile Arg Val Glu Gly Ile Val Gln Gly Val Gly 1 5 10 15 Phe Arg Tyr Phe Thr Arg Arg Ile Ala Arg Thr Leu Gly Ile Lys Gly 20 25 30 Tyr Val Met Asn Met Asp Asp Gly Ser Val Phe Ile His Ala Glu Gly 35 40 45 Glu Glu Glu Lys Leu Arg Arg Phe Leu Asn Glu Val Ser Lys Gly Pro 50 55 60 Pro Ala Ala Val Val Thr Asn Ile Ser Val Glu Glu Thr Ser Pro Glu 65 70 75 80 Gly Tyr Glu Asp Phe Ala Ile Arg Tyr Tyr 85 90 <210> 207 <211> 401 <212> PRT <213> Thermotoga neapolitana <400> 207 Met Leu Ser Phe Phe Cys Glu Thr Pro Phe Gln Ile Leu Pro Tyr Gly 1 5 10 15 Lys Glu Glu Leu Asp Ser Met Gln Leu Glu Arg Leu Asp Glu Gly Leu 20 25 30 Phe Tyr Pro His Tyr Asn Gly Tyr Ser Ile Val Asn Phe Ser Asn Thr 35 40 45 Ile Leu Ser Ile Phe Gly Glu Lys Thr Leu His Arg Pro Phe Pro Leu 50 55 60 Pro Glu Arg Phe Leu Glu Asn Val Arg Lys Val Val Val Leu Val Val 65 70 75 80 Asp Ala Leu Ser Tyr Asp Val Phe Ser Asn Thr Ile Gln Asp Asp Asp 85 90 95 Val Gln Val Phe Pro Cys Ser Ser Val Phe Pro Thr Thr Thr Ala Ala 100 105 110 Ala Leu Pro Ser Leu Tyr Ser Gly Leu Thr Pro Leu Glu His Gly Phe 115 120 125 Leu Gly Tyr Ile Leu Tyr Leu Arg Glu Val Gly Ser Pro Val Asn Met 130 135 140 Ile Glu Met Ala Pro Pro Gly Phe Pro Arg Glu Ser Val Leu Arg Ile 145 150 155 160 His Glu Phe Arg Phe Thr Thr Ile Phe Gln Arg Leu Lys Val Arg Ser 165 170 175 Phe Phe Leu Val Pro Arg Tyr Leu Leu Gly Thr Gly Phe Ser Arg Leu 180 185 190 Met Ser Gln Gly Ala Glu Gln Ile Gly Phe Ser Ser Phe Gly Asp Met 195 200 205 Ile Glu Lys Thr Leu Glu Ile Leu Ala Asn Gln Glu Thr Val Leu Val 210 215 220 Phe Ala Tyr Trp Pro Ser Leu Asp Ser Ile Ala His Lys Ser Gly Val 225 230 235 240 Gly Arg Ala Tyr Phe Arg Glu Leu Lys Trp Leu Tyr Arg Ile Leu Lys 245 250 255 Glu Glu Leu Ile Arg Arg Leu Lys Ser Asp Thr Leu Phe Phe Met Val 260 265 270 Ser Asp His Gly Gln Ile Ser Thr Pro Pro Glu Arg Glu Val Trp Trp 275 280 285 Asp Ser Phe Ser Glu Val Met Arg Phe Leu Asp Arg Pro Pro Ala Gly 290 295 300 Glu Gln Arg Met Met Tyr Leu Tyr Thr Lys Arg Lys Lys Ala Leu Val 305 310 315 320 Glu Tyr Leu Val Glu Lys Tyr Ala Asp Phe Ala Ile Phe Ile Asp Ala 325 330 335 Arg Arg Ala Thr Arg Leu Phe Gly Ile Gly Lys Ser His Pro Glu Phe 340 345 350 Phe His Arg Ile Gly Asp Leu Ile Leu Ile Thr Lys Glu Asn Phe Ser 355 360 365 Phe Asn Tyr Arg Tyr Thr Gly Lys Glu Glu Ser Leu Ser Gly Arg His 370 375 380 Gly Ser Leu Thr His Gln Glu Leu Val Val Pro Leu Val Val Tyr Arg 385 390 395 400 Arg <210> 208 <211> 229 <212> PRT <213> Thermotoga neapolitana <400> 208 Met Lys Ala Asp Leu His Val His Thr Cys Leu Ser Pro Cys Ala Asp 1 5 10 15 Leu Leu Met Ile Pro Pro Val Ile Glu Lys Ala Glu Val Asp Ile Leu 20 25 30 Gly Ile Val Asp His Asn Ser Ala Arg Asn Ala Leu Ala Phe Ser Lys 35 40 45 Met Lys Lys Leu Val Ile Pro Gly Val Glu Ile Gln Thr Val Glu Asp 50 55 60 Val His Leu Leu Gly Phe Phe Val Asn Phe Glu Asp Ala Leu Lys Leu 65 70 75 80 Thr Glu Val Leu Tyr Glu His Leu Pro Arg Leu Lys His Asp His Glu 85 90 95 Lys Met Gly Tyr Gln Leu Leu Val Asn Glu Asp Gly Asp Tyr Ile Gly 100 105 110 Tyr Glu Asn Met Pro Leu Gly Phe Pro Ala Asn Leu Thr Ile Ser Gln 115 120 125 Ala Val Asp Leu Val Leu Ser Phe Arg Gly Val Pro Ala Tyr Ala His 130 135 140 Val Glu Arg Arg Phe Gly Val Leu Tyr Gln Leu Gly Phe Phe Pro Asn 145 150 155 160 Leu Asp Val Pro Val Ala Glu Val Val Ser Glu Glu Gly Arg Ser Lys 165 170 175 Ala Glu Gly Arg Phe Arg Val Ile Val Ser Ser Asp Ala His Phe Pro 180 185 190 Asp Glu Val Gly Arg Arg Tyr Ile Glu Leu Lys Gly Lys Pro Asp Ser 195 200 205 Pro Lys Lys Val Leu Asp Gln Ile Leu Ala Gly Glu Tyr Thr Leu Gly 210 215 220 Gly Val Leu Asp Trp 225 <210> 209 <211> 122 <212> PRT <213> Thermotoga neapolitana <400> 209 Met Leu Asp Val Trp Lys Ile Phe Arg Ser Thr Pro Phe Thr Thr Ala 1 5 10 15 Val Val Ser Phe Leu Val Ala Gln Phe Ile Lys Phe Leu Ile Lys Arg 20 25 30 Asp Val Lys Met Leu Lys Ser Tyr Gly Gly Met Pro Ser Gly His Val 35 40 45 Ala Thr Val Ser Gly Leu Ala Trp Ser Leu Ala Arg Ser Thr Gly Phe 50 55 60 Asp Ser Pro Tyr Thr Ser Ile Ala Ser Ile Phe Leu Val Ile Ile Phe 65 70 75 80 Met Asp Ala Ile Val Leu Arg Pro Ala Val Lys Lys Asp Leu Gly His 85 90 95 Ser Phe Leu Glu Ala Leu Ala Gly Phe Gly Leu Gly Met Leu Ile Ala 100 105 110 His Leu Phe Pro Ala Arg Leu His Leu Trp 115 120 <210> 210 <211> 181 <212> PRT <213> Thermotoga neapolitana <400> 210 Met Thr Val Lys Phe Tyr Glu Gln Lys Ile Glu Ser Lys Arg Val Phe 1 5 10 15 Glu Gly Lys Met Ile Ser Val Arg Val Asp Arg Val Arg Leu Pro Asn 20 25 30 Gly Glu Glu Ser Thr Arg Glu Val Val Asp His Pro Gly Ala Val Val 35 40 45 Ile Val Pro Val Leu Gly Glu Glu Ile Ile Phe Val Glu Gln Tyr Arg 50 55 60 Tyr Pro Ile Glu Gln Met Leu Leu Glu Leu Pro Ala Gly Lys Met Asp 65 70 75 80 Pro Gly Glu Ser Pro Glu Glu Cys Ala Lys Arg Glu Leu Glu Glu Glu 85 90 95 Thr Gly Tyr Arg Ala Lys Arg Phe Ser Tyr Leu Gly Lys Ile Phe Thr 100 105 110 Thr Pro Gly Phe Thr Thr Glu Val Ile His Ile Phe Ala Ala Glu Glu 115 120 125 Leu Glu Lys Thr Thr Gln Asn Thr Asp Pro Asp Glu Phe Ile Glu Val 130 135 140 Lys Lys Ile Pro Val Glu Lys Val Leu Ser Leu Leu Arg Asn Ala Glu 145 150 155 160 Ile Glu Asp Ser Lys Thr Ile Cys Ala Leu Thr Arg Tyr Phe Leu Ser 165 170 175 Lys Gly Val Ile Gly 180 <210> 211 <211> 224 <212> PRT <213> Thermotoga neapolitana <400> 211 Met Lys Lys Ala Val Leu Phe Asp Leu Asp Gly Thr Ile Leu Asp Phe 1 5 10 15 Glu Lys Ser Glu Glu Thr Ala Leu Lys Lys Thr Phe Leu Arg His Gly 20 25 30 Ile Pro Leu Thr Glu Glu Gln Val Leu Leu Tyr Lys Ser Ile Asn Arg 35 40 45 Lys Trp Trp Lys Met Leu Ala Glu Lys Lys Val Ser Lys Glu Glu Val 50 55 60 Val Val Ala Arg Phe Glu Glu Phe Leu Gly Glu Ile Gly Ser Leu Leu 65 70 75 80 Asp Pro Glu Glu Val Ala Lys Glu Tyr Leu Glu Phe Leu Ser Glu Glu 85 90 95 Ala Tyr Phe Leu Pro Gly Ala Glu Asp Phe Leu Lys Glu Leu Lys Arg 100 105 110 Asn Gly Phe Arg Met Ala Ala Val Thr Asn Gly Val Arg Phe Val Gln 115 120 125 Glu Arg Arg Ser Lys Lys Leu Gly Leu Glu Arg Phe Phe Glu Phe Val 130 135 140 Leu Thr Ser Glu Glu Val Gly Val Glu Lys Pro Asp Pro Arg Ile Phe 145 150 155 160 Trp Ile Ala Leu Glu Arg Met Gly Leu Lys Lys Glu Asp Ala Leu Tyr 165 170 175 Val Gly Asp Asp Pro Ala Ser Asp Leu Glu Gly Ala Arg Asn Ala Gly 180 185 190 Ile Asp Phe Val Leu Phe Ser Pro Ser Gly Asp Val Ser Arg Glu Phe 195 200 205 Pro Val Val Arg Asn Phe Glu Glu Leu Arg Glu Ile Leu Arg Ile Leu 210 215 220 <210> 212 <211> 204 <212> PRT <213> Thermotoga neapolitana <400> 212 Met Asn Ile Ser Glu Arg Gln Lys Asp Leu Leu Lys Glu Ile Gly Asn 1 5 10 15 Ile Gly Ala Gly Asn Ala Ala Thr Ala Ile Ser Tyr Met Val Asn Lys 20 25 30 Lys Val Glu Ile Ser Val Pro Thr Val Glu Ile Ile Pro Leu Ser Asp 35 40 45 Val Ile Phe Val Ala Lys Asp Pro Glu Glu Ile Val Val Gly Val Lys 50 55 60 Met Pro Val Thr Gly Glu Ile Glu Gly Asn Val Leu Leu Ile Met Gly 65 70 75 80 Thr Gln Val Val Lys Lys Ile Leu Glu Ile Leu Ile Gly His Ala Pro 85 90 95 Glu Asn Leu Leu Gln Leu Asp Glu Phe Ala Ala Ser Ala Leu Gln Glu 100 105 110 Val Gly Asn Ile Met Cys Gly Thr Tyr Val Ser Ala Leu Ala Asp Phe 115 120 125 Leu Gly Phe Lys Ile Asp Thr Leu Pro Pro Gln Leu Val Val Asp Met 130 135 140 Ile Ser Ala Ile Phe Ala Glu Val Ser Ile Glu Glu Leu Gly Glu Ser 145 150 155 160 Val Asp Asp Gln Val Val Phe Val Glu Thr Ser Leu Thr Val Glu Glu 165 170 175 Glu Glu Pro Leu Thr Ser Tyr Leu Met Leu Val Pro Lys Pro Gly Tyr 180 185 190 Leu Lys Lys Ile Phe Glu Arg Met Gly Val Gln Glu 195 200 <210> 213 <211> 236 <212> PRT <213> Thermotoga neapolitana <400> 213 Met Glu Met Leu Leu Gly Ile Val Gln Gly Leu Thr Glu Phe Leu Pro 1 5 10 15 Val Ser Ser Ser Gly His Leu Val Leu Phe Ser His Val Phe Lys Met 20 25 30 Asp Leu Asn Ala Tyr Gln Thr Ala Ile Leu His Leu Gly Thr Leu Ala 35 40 45 Ala Val Val Val Phe Ala Leu Glu Gly Ile Arg Arg Ser Leu Arg Asn 50 55 60 Trp Arg Val Ile Leu Asn Leu Val Ile Ser Thr Ile Pro Ala Gly Val 65 70 75 80 Phe Gly Val Leu Phe Glu Ser Arg Val Asp Glu Ala Phe Ser Ser Pro 85 90 95 Val Val Leu Pro Leu Phe Phe Ser Phe Thr Ala Leu Val Leu Leu Phe 100 105 110 Thr Ser Ser Phe Ser Gly Glu Lys Arg Met Glu Glu Met Thr Ile Leu 115 120 125 Asp Ala Thr Leu Val Gly Leu Ala Gln Val Leu Ala Leu Phe Pro Gly 130 135 140 Val Ser Arg Ser Gly Ile Thr Ile Ala Thr Leu Leu Phe Leu Lys Tyr 145 150 155 160 Arg Arg Glu Asp Ala Leu Gln Tyr Ser Phe Leu Met Ser Ile Pro Val 165 170 175 Val Leu Gly Ala Gly Ile Leu Gly Met Glu Lys Gly Asn Ile Ser Leu 180 185 190 Thr Ala Pro Phe Phe Ala Phe Leu Ser Gly Ile Leu Ala Leu Tyr Ile 195 200 205 Leu Ser Arg Ser Val Arg Ser Gly Lys Met Trp Gln Phe Ala Tyr Tyr 210 215 220 Cys Leu Phe Val Ala Ile Val Ser Tyr Phe Val Gly 225 230 235 <210> 214 <211> 233 <212> PRT <213> Thermotoga neapolitana <400> 214 Met Ile Asp Met His Leu His Ser Thr Phe Ser Tyr Asp Gly Lys Ala 1 5 10 15 Glu Val Glu Asp Ile Ile Ala Gln Val Gln Lys Leu Gly Ile Asn His 20 25 30 Phe Cys Ile Thr Asp His Tyr Glu Tyr Glu Asn Gly Lys Leu Val His 35 40 45 Asp Phe Asn Val Glu Glu Tyr Phe Leu Thr Met Asp Lys Phe Asp Leu 50 55 60 Pro Arg Gly Ala Glu Ile Ser Trp Asp Gly Val Lys Glu Ala Ile Phe 65 70 75 80 Pro Glu Gly Phe Asp Tyr Leu Leu Leu Gly Val His Arg Tyr Asp Glu 85 90 95 Asn Leu Pro Pro Asp Glu Leu Ala Arg Asp Tyr Leu Glu Arg Thr Leu 100 105 110 Tyr Val Met Glu Arg Val Glu Phe His Thr Leu Ala His Leu Asp Tyr 115 120 125 Pro Ala Arg Tyr Ala Asn Ala Asp Phe Lys Ala Asn Arg Asp Leu Ile 130 135 140 Glu Lys Ile Leu Arg Phe Leu Val Ala Arg Glu Lys Ile Leu Glu Val 145 150 155 160 Asn Thr Ala Gly Leu Phe Lys His Gly Lys Pro Asn Pro Asp Tyr Trp 165 170 175 Ile Leu Glu Met Tyr Trp Asp Leu Gly Gly Arg Phe Ile Thr Ile Gly 180 185 190 Ser Asp Ala His Glu Val Gln His Ile Gly Arg Gly Ile Glu Glu Val 195 200 205 Met Ser Lys Leu Arg Arg Phe Asn Phe Glu Tyr Val Val Val Glu Gly 210 215 220 Lys Lys Leu Phe Thr Lys Lys Leu Arg 225 230 <210> 215 <211> 227 <212> PRT <213> Thermotoga neapolitana <400> 215 Met Val Asn Val Val Met Val Pro Cys Ser Val Val Lys Asp Glu Thr 1 5 10 15 Val Val Val Val Asp Val Leu Arg Ala Thr Ser Thr Ile Val Thr Ala 20 25 30 Leu Ala Asn Gly Ala Lys Glu Val Ile Pro Val Lys Thr Val Glu Glu 35 40 45 Ala Leu Lys Arg Lys Glu Arg Asp Val Leu Val Cys Gly Glu Arg Asn 50 55 60 Ala Lys Lys Ile Glu Gly Phe Asp Leu Gly Asn Ser Pro Leu Glu Tyr 65 70 75 80 Lys Ser Glu Leu Val Leu Gly Lys Thr Ile Val Leu Thr Thr Thr Asn 85 90 95 Gly Thr Gln Val Ile Glu Arg Val Glu Gly Asp Ser Val Ile Ala Ala 100 105 110 Ser Phe Leu Asn Val Ser Ala Val Val Glu Tyr Leu Lys Glu Lys Glu 115 120 125 Asn Ile Thr Ile Val Cys Ala Gly Thr Ser Gly Asp Phe Ser Leu Glu 130 135 140 Asp Phe Leu Leu Ala Gly Met Ile Val Lys Arg Met Lys Arg Glu Asp 145 150 155 160 Leu Leu Asp Gly Ala Leu Val Val Met Arg Tyr Phe Glu Ser Val Gln 165 170 175 Asn Ile Arg Glu Glu Ile Lys Arg Phe Ser Ser His Ala Lys Arg Leu 180 185 190 Ile Ser Leu Gly Phe Glu Lys Asp Val Asp Phe Cys Thr Thr Glu Asp 195 200 205 Leu Phe His Glu Val Pro Val Leu Val Asn Gly Ala Phe Thr Leu Lys 210 215 220 Glu Ala Arg 225 <210> 216 <211> 209 <212> PRT <213> Thermotoga neapolitana <400> 216 Met Thr Tyr Ala Val Gly Asp Ile His Gly Cys Phe Phe Ala Leu Lys 1 5 10 15 Ala Leu Leu Glu Lys Leu Pro Leu Gly Lys Glu Asp Glu Leu Val Phe 20 25 30 Leu Gly Asp Tyr Val Asp Arg Gly Pro Asn Ser Lys Glu Val Val Glu 35 40 45 Phe Leu Met Glu Leu Ser Lys His His Arg Cys Ile Phe Leu Arg Gly 50 55 60 Asn His Glu Glu Met Leu Leu Asn Cys Val Lys Asn His Ser Gly Cys 65 70 75 80 Asp Leu Trp Tyr Phe Asn Gly Ala Arg Ser Thr Val Glu Ser Phe Gly 85 90 95 Gly Ile Asp Glu Ile Arg Lys Tyr Leu Asp Phe Phe Glu Ser Thr Val 100 105 110 Tyr Tyr Tyr Glu Lys Gly Asn Phe Val Phe Val His Gly Gly Val Lys 115 120 125 Pro Gly Ile Pro Leu Glu Glu Gln Asp Pro Phe Asp Leu Val Trp Ile 130 135 140 Arg Glu Glu Phe Ile Tyr Ser Glu Asn Pro Leu Pro Gly Lys Thr Val 145 150 155 160 Val Phe Gly His Thr Pro Leu Glu Lys Pro Tyr Val Ser Ser Asp Lys 165 170 175 Ile Gly Ile Asp Thr Gly Cys Val Tyr Gly Gly Lys Leu Thr Ala Phe 180 185 190 Arg Val Glu Asp Arg Thr Phe Phe Gln Val Glu Cys Ile Gly Arg Arg 195 200 205 Trp <210> 217 <211> 264 <212> PRT <213> Thermotoga petrophilia <400> 217 Met Phe Val Phe Asp Leu Asp Gly Thr Leu Leu Asn Asp Ser Leu Glu 1 5 10 15 Ile Ser Glu Lys Asp Arg Arg Ala Ile Glu Arg Leu Ser Arg Lys Cys 20 25 30 Arg Val Val Phe Ala Ser Gly Arg Met Leu Val Ser Thr Leu Asn Val 35 40 45 Glu Lys Lys Tyr Phe Gly Arg Thr Phe Pro Thr Ile Ala Tyr Asn Gly 50 55 60 Ala Met Val Tyr Ile Pro Glu Glu Gly Val Ile Leu Asn Glu Lys Ile 65 70 75 80 Pro Pro Glu Val Ala Lys Asp Ile Val Glu Tyr Val Lys Gln Phe Asn 85 90 95 Val His Trp Gln Ala Tyr Ile Asp Asp Val Leu Tyr Ser Glu Lys Asp 100 105 110 Asn Glu Glu Ile Arg Gly Tyr Ala Lys His Ser Ser Val Glu Tyr Arg 115 120 125 Val Glu Pro Lys Leu Leu Glu Leu Val Ser Lys Val Gly Thr Thr Lys 130 135 140 Ile Leu Leu Ile Asp Thr Pro Glu Lys Leu Asp Glu Leu Lys Lys Ile 145 150 155 160 Leu Leu Glu Lys Phe Asn Asn Val Val Lys Val Phe Lys Ser Phe Pro 165 170 175 Thr Tyr Leu Glu Ile Val Pro Glu Asn Val Asp Lys Gly Lys Ala Leu 180 185 190 Arg Phe Leu Arg Glu Arg Met Gly Trp Lys Lys Glu Glu Ile Val Val 195 200 205 Phe Gly Asp Asn Glu Asn Asp Leu Phe Met Phe Glu Glu Ala Gly Leu 210 215 220 Arg Val Ala Met Gly Asn Ala Ile Asp Lys Val Lys Glu Ala Ala Asp 225 230 235 240 Val Val Thr Leu Thr Asn Asn Asp Ser Gly Val Ser His Val Leu Glu 245 250 255 Leu Ile Ser Thr Asp Cys Leu Asp 260 <210> 218 <211> 208 <212> PRT <213> Thermus amyloliquefaciens <400> 218 Met Leu Lys Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp Thr 1 5 10 15 Asp Pro Leu His Leu Gln Ala Trp Arg Glu Ala Leu Ala Pro Trp Gly 20 25 30 Leu Gln Val Asp Glu Ala Phe Tyr Arg Lys Arg Ile Ser Gly Arg Leu 35 40 45 Asn Pro Asp Ile Val Gln Asp Leu Leu Gly Leu Glu Gly Glu Ala Ala 50 55 60 Gln Arg Leu Ile Glu Ala Lys Glu Ala Arg Phe Arg Glu Leu Ala Gln 65 70 75 80 Asp Leu Lys Pro Thr Pro Gly Leu His Gly Leu Leu Ala Lys Ala Glu 85 90 95 Ala Gln Gly Leu Thr Trp Gly Val Val Thr Asn Ala Pro Arg Glu Asn 100 105 110 Ala Leu His Val Leu Arg Ala Leu Gly Leu Asn Pro Pro Leu Leu Val 115 120 125 Leu Ala Glu Glu Val Gly Arg Gly Lys Pro Asp Pro Leu Pro Tyr Arg 130 135 140 Val Ala Leu Glu Arg Leu Gly Ile Glu Pro Gly Glu Ala Leu Ala Phe 145 150 155 160 Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly Ile Pro 165 170 175 Thr Tyr Ala Leu Leu Thr Gly His Gly Lys Glu Ala Leu Leu Glu Ala 180 185 190 Gly Ala Leu Glu Ala Leu Arg Asp Phe Gln Gln Ile Val Asp Leu Leu 195 200 205 <210> 219 <211> 260 <212> PRT <213> Thermus filiformis <400> 219 Met Lys Val Ser Gln Ala His Leu Glu Ala Ala Ile Asp Ala Ala Phe 1 5 10 15 Leu Ala Gln Gly Ile His Thr Tyr Tyr Leu Glu Arg Gly Phe Thr Gln 20 25 30 Lys Thr Lys Ser Gly Pro Thr Asp Leu Val Thr Gln Ala Asp Lys Glu 35 40 45 Ser Glu Glu Ala Ile Lys Asp Leu Leu Leu Ser Arg Phe Pro Asp His 50 55 60 Gly Phe Leu Gly Glu Glu Gly Gly Gly Asp Ala Gly Gly Gly Val Arg 65 70 75 80 Trp Val Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe 85 90 95 Pro Phe Tyr Gly Val Ser Ile Ala Leu Glu Val Glu Gly Arg Val Glu 100 105 110 Leu Gly Val Val Leu Asp Thr Ala Arg Gly Glu Leu Phe Thr Ala Leu 115 120 125 Arg Gly Gln Gly Ala Tyr Leu Asn Gly Arg Pro Ile Arg Val Thr Glu 130 135 140 Arg Thr Glu Leu Leu Gly Ser Leu Leu Ala Thr Gly Phe Pro Tyr Asp 145 150 155 160 Val Ala Arg Asp Pro Glu Asn Leu Thr Tyr Phe Glu Arg Ala Leu Arg 165 170 175 Arg Gly Leu Leu Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala 180 185 190 Tyr Val Ala Ala Gly Arg Leu Asp Gly Phe Trp Glu Val Lys Leu Asn 195 200 205 Pro Trp Asp Val Ala Ala Gly Trp Leu Leu Val Glu Glu Ala Gly Gly 210 215 220 Arg Val Thr Asp Leu Glu Gly Glu Ala Tyr Arg Leu Gly His Arg Tyr 225 230 235 240 Ile Val Ala Thr Asn Gly Arg Ile His Gln Ala Leu Leu Ala Val Leu 245 250 255 Arg Gly Thr Met 260 <210> 220 <211> 208 <212> PRT <213> Thermus thermophilus <400> 220 Met Leu Lys Ala Val Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp Thr 1 5 10 15 Asp Pro Leu His Leu Leu Ala Trp Arg Glu Ala Leu Ala Pro Tyr Gly 20 25 30 Leu Glu Val Asp Pro Ile Phe Tyr Arg Lys Arg Ile Ser Gly Arg Leu 35 40 45 Asn Pro Glu Ile Val Arg Asp Leu Leu Gly Leu Glu Gly Glu Glu Ala 50 55 60 Glu Arg Leu Ile Ala Ala Lys Glu Ala Arg Phe Arg Ala Leu Ala Gln 65 70 75 80 Gly Leu Arg Pro Thr Pro Gly Leu Ala Glu Phe Leu Glu His Ile Arg 85 90 95 Glu Lys Gly Leu Leu Trp Gly Val Val Thr Asn Ala Pro Lys Glu Asn 100 105 110 Ala Arg His Val Leu Glu Ala Leu Gly Leu Arg Pro Pro Leu Leu Val 115 120 125 Leu Ala Glu Glu Val Gly Arg Gly Lys Pro Asp Pro Leu Pro Tyr Arg 130 135 140 Val Ala Leu Arg Arg Leu Gly Val Ala Pro Glu Glu Ala Leu Ala Phe 145 150 155 160 Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly Ile Pro 165 170 175 Thr Tyr Gly Leu Leu Thr Gly His Glu Ala Glu Ala Leu Arg Glu Ala 180 185 190 Gly Ala Arg Gly Val Phe Arg Asp Phe Arg Glu Ala Leu Gly Leu Leu 195 200 205 <210> 221 <211> 264 <212> PRT <213> Thermus thermophilus <400> 221 Met Ile Gly Arg Arg His Pro Tyr Tyr Pro Tyr Leu Glu Ala Ala Leu 1 5 10 15 Glu Ala Ala Ser Leu Ala Arg Gly Ile His Leu Tyr Tyr Leu Glu Lys 20 25 30 Gly Phe Thr Glu Gly Thr Lys Ser Gly Pro Thr Asp Leu Val Thr Gln 35 40 45 Ala Asp Arg Glu Ala Glu Glu Ala Val Lys Gly Leu Leu Leu Ser Arg 50 55 60 Phe Pro Glu Ala Gly Phe Leu Gly Glu Glu Gly Gly Ser Glu Gly Gly 65 70 75 80 Lys Ala Leu Arg Phe Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr 85 90 95 Ala His Gly Phe Pro Phe Phe Ala Val Ser Ile Ala Leu Glu Ala Glu 100 105 110 Gly Ala Ile Gln Met Gly Val Val Met Asp Thr Ala Arg Gly Glu Val 115 120 125 Phe Tyr Ala Leu Arg Gly Glu Gly Ala Tyr Leu Asn Gly Arg Pro Ile 130 135 140 Arg Val Thr Gly Arg Glu Ser Leu Val Gly Ser Leu Leu Ala Thr Gly 145 150 155 160 Phe Pro Tyr Asp Val Ala Lys Asp Pro Glu Asn Leu Thr Tyr Phe Glu 165 170 175 Arg Ala Leu Gly Lys Gly Leu Leu Val Arg Arg Pro Gly Ala Ala Ala 180 185 190 Leu Asp Leu Ala Tyr Val Ala Ala Gly Arg Leu Glu Gly Phe Trp Glu 195 200 205 Val Lys Leu Asn Pro Trp Asp Val Ala Ala Gly Trp Leu Leu Val Glu 210 215 220 Glu Ala Gly Gly Arg Val Thr Asp Leu Glu Gly Asn Pro Tyr Arg Leu 225 230 235 240 Gly Ser Arg Tyr Ile Leu Ala Thr Asn Gly Arg Val His Glu Ala Leu 245 250 255 Arg Arg Thr Leu Leu Gly Leu Asp 260 <210> 222 <211> 229 <212> PRT <213> Truepera radiovictrix <400> 222 Met Gly His Pro Lys Ala Val Leu Leu Asp Val Asp Gly Thr Leu Val 1 5 10 15 Asp Ser Asn Asp Ala His Ala Arg Ala Tyr Val Asp Ala Leu Lys Ala 20 25 30 Tyr Gly Tyr Asp Val Pro Phe Glu Lys Val Arg Pro Leu Ile Gly Met 35 40 45 Gly Gly Asp Lys Leu Leu Pro Gln Val Ile Asp Val Glu Ser Asp Ala 50 55 60 Pro Leu Arg Glu Glu Ile Asp Ala Arg Arg Gly Glu Ile Phe Lys Ser 65 70 75 80 Arg Tyr Leu Pro Asp Ile Lys Pro Phe Pro Gly Val Arg Glu Leu Leu 85 90 95 Glu Arg Leu Arg Arg Asp Gly Leu Thr Leu Val Ala Ala Ser Ser Ala 100 105 110 Lys Glu Asp Glu Ala Asp Ala Leu Leu Glu Arg Ala Gly Val Ala Asp 115 120 125 Leu Ile Asp Ala Arg Thr Ser Ser Asp Asp Ala Glu Asn Ser Lys Pro 130 135 140 Asp Pro Asp Ile Leu Gln Ala Ala Leu Glu Arg Ala Gly Val Ser Ala 145 150 155 160 Gln Glu Ala Leu Leu Leu Gly Asp Thr Pro Tyr Asp Leu Gln Ala Ala 165 170 175 Ala Pro Leu Gly Leu Gly Val Val Ala Leu Arg Cys Gly Gly Trp Arg 180 185 190 Asp Glu Asp Leu Leu Gly Ala Leu Ala Val Tyr Asp Asp Pro Gln Ala 195 200 205 Leu Leu Ala Gly Tyr Asp Ala Ser Pro Leu Ala Pro Glu Pro Ala Ser 210 215 220 Ala Pro Gly Asp Val 225

Claims (21)

  1. 삭제
  2. 삭제
  3. 서열번호 1, 6, 9, 12 내지 14, 17, 18, 20 내지 23, 25, 26, 28, 29, 37 내지 53, 55 내지 57, 59 내지 61, 64 내지 66, 69, 70, 72, 73, 76, 77, 80, 81, 85, 88, 91, 93, 95, 99, 100, 102, 104, 105, 108, 114, 116, 117, 122, 131, 133, 134, 136, 142 내지 148, 150 내지 152, 154 내지 156, 159 내지 164, 167, 169, 172, 173, 175, 177, 184 내지 187, 189, 191, 192, 195, 198, 205, 210, 211, 214, 216, 217, 및 221 중 어느 하나의 아미노산 서열로 이루어진 싸이코스-6-인산의 탈인산화 효소, 이를 발현하는 미생물, 또는 상기 미생물의 배양물을 포함하는 싸이코스 생산용 조성물.
  4. 제3항에 있어서, 상기 효소는 서열번호 1, 6, 9, 12, 26, 29, 38 내지 43, 45 내지 53, 56, 57, 59, 60, 64 내지 66, 69, 70, 72, 76, 80, 81, 91, 93, 95, 99, 100, 102, 114, 116, 117, 131, 134, 136, 142, 145, 146, 148, 164, 167, 169, 172, 177, 184 내지 187, 189, 191, 192, 211, 217, 및 221 중 어느 하나의 아미노산 서열로 이루어진 것인, 싸이코스 생산용 조성물.
  5. 제3항에 있어서, 상기 효소는 싸이코스-6-인산 선택적 활성을 나타내는 것인, 싸이코스 생산용 조성물.
  6. 삭제
  7. 삭제
  8. 삭제
  9. 제3항에 있어서, 상기 조성물은 인산당을 추가로 포함하는 것인, 조성물.
  10. 서열번호 1, 6, 9, 12 내지 14, 17, 18, 20 내지 23, 25, 26, 28, 29, 37 내지 53, 55 내지 57, 59 내지 61, 64 내지 66, 69, 70, 72, 73, 76, 77, 80, 81, 85, 88, 91, 93, 95, 99, 100, 102, 104, 105, 108, 114, 116, 117, 122, 131, 133, 134, 136, 142 내지 148, 150 내지 152, 154 내지 156, 159 내지 164, 167, 169, 172, 173, 175, 177, 184 내지 187, 189, 191, 192, 195, 198, 205, 210, 211, 214, 216, 217, 및 221 중 어느 하나의 아미노산 서열로 이루어진 싸이코스-6-인산 탈인산화 효소, 이를 발현하는 미생물, 또는 상기 미생물의 배양물을 싸이코스-6-인산과 접촉시켜 싸이코스-6-인산을 싸이코스로 전환하는 단계를 포함하는, 싸이코스 제조방법.
  11. 제10항에 있어서, 상기 방법은 싸이코스-6-인산을 싸이코스로 전환하는 단계 이전, 과당-6-인산(fructose-6-phosphate)에 과당-6-인산-3-에피머화 효소, 이를 발현하는 미생물 또는 상기 미생물의 배양물을 접촉시켜, 상기 과당-6-인산을 싸이코스-6-인산으로 전환하는 단계를 추가적으로 포함하는, 싸이코스 제조방법.
  12. 제11항에 있어서, 상기 방법은 상기 과당-6-인산을 싸이코스-6-인산으로 전환하는 단계 이전, 포도당-6-인산(Glucose-6-phosphate)에 포도당-6-인산-이성화효소, 이를 발현하는 미생물 또는 상기 미생물의 배양물을 접촉시켜, 상기 포도당-6-인산을 과당-6-인산으로 전환하는 단계를 추가적으로 포함하는, 싸이코스 제조방법.
  13. 제12항에 있어서, 상기 방법은 상기 포도당-6-인산을 과당-6-인산으로 전환하는 단계 이전, 포도당-1-인산(Glucose-1-phosphate)에 포스포글루코무타아제, 이를 발현하는 미생물 또는 상기 미생물의 배양물을 접촉시켜, 상기 포도당-1-인산을 포도당-6-인산으로 전환하는 단계를 추가적으로 포함하는, 싸이코스 제조방법.
  14. 제12항에 있어서, 상기 방법은 상기 포도당-6-인산을 과당-6-인산으로 전환하는 단계 이전, 포도당(Glucose)에 폴리포스페이트 포도당 인산화 효소, 이를 발현하는 미생물 또는 상기 미생물의 배양물, 및 폴리포스페이트를 접촉시켜, 상기 포도당을 포도당-6-인산으로 전환하는 단계를 추가적으로 포함하는, 싸이코스 제조방법.
  15. 제13항에 있어서, 상기 방법은 상기 포도당-1-인산을 포도당-6-인산으로 전환하는 단계 이전, 전분, 말토덱스트린, 수크로스 또는 이의 조합에 α -글루칸 포스포릴라아제, 전분 포스포릴라아제, 말토덱스트린 포스포릴라아제 또는 수크로오스 포스포릴라아제; 이를 발현하는 미생물; 또는 상기 미생물의 배양물, 및 포스페이트를 접촉시켜, 상기 전분, 말토덱스트린, 수크로스 또는 이의 조합을 포도당-1-인산으로 전환하는 단계를 추가적으로 포함하는, 싸이코스 제조방법.
  16. 제15항에 있어서, 상기 전분, 말토덱스트린, 수크로스 또는 이의 조합을 포도당-1-인산으로 전환하는 단계는 상기 전분, 말토덱스트린, 수크로스 또는 이의 조합에 α-아밀라아제, 풀루란아제, 이소아밀레이즈, α-글루카노트랜스퍼라아제, 글루코아밀라아제 또는 수크라아제; 이를 발현하는 미생물; 또는 상기 미생물의 배양물을 접촉시켜, 상기 전분, 말토덱스트린, 수크로스 또는 이의 조합을 말토올리고당 또는 포도당으로 전환하는 단계를 추가적으로 포함하는, 싸이코스 제조방법.
  17. 전분, 말토덱스트린, 수크로스 또는 이의 조합, 및 포스페이트에 (a) 싸이코스-6-인산 탈인산화 효소; 과당-6-인산-3-에피머화효소; 포도당-6-인산-이성화효소; 포스포글루코무타아제 또는 포도당 인산화 효소; 및 α-글루칸 포스포릴라아제, 전분 포스포릴라아제, 말토덱스트린 포스포릴라아제, 수크로오스 포스포릴라아제, α-아밀라아제, 풀루란아제, 이소아밀라아제, 글루코아밀라아제, α-글루카노트랜스퍼라아제, 폴리포스페이트 글루코카이네이즈 또는 수크라아제; 또는 (b) 상기 항목 (a)의 효소를 발현하는 미생물 또는 상기 미생물의 배양물을 접촉시키는 단계를 포함하는, 싸이코스 제조방법으로서, 상기 싸이코스-6-인산 탈인산화 효소는 서열번호 1, 6, 9, 12 내지 14, 17, 18, 20 내지 23, 25, 26, 28, 29, 37 내지 53, 55 내지 57, 59 내지 61, 64 내지 66, 69, 70, 72, 73, 76, 77, 80, 81, 85, 88, 91, 93, 95, 99, 100, 102, 104, 105, 108, 114, 116, 117, 122, 131, 133, 134, 136, 142 내지 148, 150 내지 152, 154 내지 156, 159 내지 164, 167, 169, 172, 173, 175, 177, 184 내지 187, 189, 191, 192, 195, 198, 205, 210, 211, 214, 216, 217, 및 221 중 어느 하나의 아미노산 서열로 이루어진 싸이코스-6-인산 탈인산화 효소인, 싸이코스 제조방법.
  18. 제11항 내지 제17항 중 어느 한 항에 있어서, 상기 접촉은 pH 5.0 내지 9.0, 40℃ 내지 80℃ 온도, 및/또는 2시간 내지 24 시간 또는 120시간 동안 실시하는, 싸이코스 제조방법.
  19. 삭제
  20. 제10항에 있어서, 상기 싸이코스-6-인산 탈인산화 효소는 서열번호 1, 6, 9, 12, 26, 29, 38 내지 43, 45 내지 53, 56, 57, 59, 60, 64 내지 66, 69, 70, 72, 76, 80, 81, 91, 93, 95, 99, 100, 102, 114, 116, 117, 131, 134, 136, 142, 145, 146, 148, 164, 167, 169, 172, 177, 184 내지 187, 189, 191, 192, 211, 217, 및 221 중 어느 하나의 아미노산 서열로 이루어진 것인, 싸이코스 제조방법.
  21. 제10항에 있어서, 상기 싸이코스-6-인산 탈인산화 효소는 싸이코스-6-인산 선택적 활성을 나타내는 것인, 싸이코스 제조방법.

KR1020180159351A 2018-12-11 2018-12-11 신규 싸이코스-6-인산의 탈인산화 효소, 이를 포함하는 싸이코스 생산용 조성물, 및 이를 이용한 싸이코스 제조방법 KR102233375B1 (ko)

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EP19895174.1A EP3882344A4 (en) 2018-12-11 2019-12-05 NOVEL PSICOSIS-6-PHOSPHORIC ACID DEPHOSPHORYLATING ENZYME, PSICOSIS PRODUCTION COMPOSITION COMPRISING THE SAME, AND PSICOSIS PRODUCTION METHOD USING THE SAME
MX2021006808A MX2021006808A (es) 2018-12-11 2019-12-05 Novedosa psicosa-6-fosfato fosfatasa, composicion para la produccion de psicosa que comprende la misma y procedimiento para la produccion de psicosa mediante el uso de la misma.
CN201980089137.XA CN113544265B (zh) 2018-12-11 2019-12-05 新阿洛酮糖-6-磷酸磷酸酶，包含其制备阿洛酮糖的组合物，和用其制备阿洛酮糖的方法
US17/312,697 US20220056426A1 (en) 2018-12-11 2019-12-05 Novel psicose-6-phosphate phosphatase, composition for producing psicose comprising the same, and method for producing psicose using the same
JP2021533165A JP2022512197A (ja) 2018-12-11 2019-12-05 新規なプシコース－６－リン酸の脱リン酸化酵素、それを含むプシコース生産用組成物、及びこれを用いたプシコース製造方法
CA3122746A CA3122746A1 (en) 2018-12-11 2019-12-05 Psicose-6-phosphate phosphatase, composition for producing psicose comprising the same, and method for producing psicose using the same
PCT/KR2019/017114 WO2020122504A1 (ko) 2018-12-11 2019-12-05 신규 싸이코스-6-인산의 탈인산화 효소, 이를 포함하는 싸이코스 생산용 조성물, 및 이를 이용한 싸이코스 제조방법
SG11202106032UA SG11202106032UA (en) 2018-12-11 2019-12-05 Novel psicose-6-phosphate phosphatase, composition for producing psicose comprising the same, and method for producing psicose using the same
BR112021011264-2A BR112021011264A2 (pt) 2018-12-11 2019-12-05 Psicose-6-fosfato fosfatase, ácido nucleico, transformante, composição para produzir psicose, e, método para produzir psicose
IL283842A IL283842A (en) 2018-12-11 2021-06-09 A new psychosis-6-phosphate phosphatase, a preparation for preparing psychosis containing the phosphatase and a method for preparing psychosis using the phosphatase
CL2021001511A CL2021001511A1 (es) 2018-12-11 2021-06-09 Novedosa psicosa-6-fosfato fosfatasa, composición para la producción de psicosa que comprende la misma y procedimiento para la producción de psicosa mediante el uso de la misma
CONC2021/0008763A CO2021008763A2 (es) 2018-12-11 2021-07-01 Enzima de desfosforilación del nuevo ácido psicosa-6-fosfórico, composición para producir psicosa que comprende la misma y método para preparar psicosa usando la misma
JP2022172294A JP2023011749A (ja) 2018-12-11 2022-10-27 新規なプシコース－６－リン酸の脱リン酸化酵素、それを含むプシコース生産用組成物、及びこれを用いたプシコース製造方法

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