CN113544265B - 新阿洛酮糖-6-磷酸磷酸酶，包含其制备阿洛酮糖的组合物，和用其制备阿洛酮糖的方法 - Google Patents

新阿洛酮糖-6-磷酸磷酸酶，包含其制备阿洛酮糖的组合物，和用其制备阿洛酮糖的方法 Download PDF

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CN113544265B
CN113544265B CN201980089137.XA CN201980089137A CN113544265B CN 113544265 B CN113544265 B CN 113544265B CN 201980089137 A CN201980089137 A CN 201980089137A CN 113544265 B CN113544265 B CN 113544265B
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朴炫俊
尹相咏
赵显国
梁成才
金昭亨
金承焕
朴逸香
孙秉三
金成俌
朴承源
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Abstract

本发明涉及一种阿洛酮糖‑6‑磷酸磷酸酶，包含所述酶的微生物，以及使用所述酶制备阿洛酮糖的方法。

Description

新阿洛酮糖-6-磷酸磷酸酶，包含其制备阿洛酮糖的组合物， 和用其制备阿洛酮糖的方法
技术领域
本发明涉及一种新型的酶，其将阿洛酮糖-6-磷酸去磷酸化，以制备阿洛酮糖，一种组合物，其包含所述酶，用于制备阿洛酮糖，一种使用所述酶制备阿洛酮糖的方法
背景技术
已知D-阿洛酮糖-3-差向异构酶(D-Psicose-3-epimerase,EC 5.1.3.30)和D-塔格糖-3-差向异构酶(D-tagatose-3-epimerase,EC5.1.3.31)是催化D-果糖的3-差向异构化以制备D-阿洛酮糖的酶。当使用该酶并通过单一酶促反应制备D-阿洛酮糖时，底物(即D-果糖)与产物(即D-阿洛酮糖)之间的反应平衡(reaction equilibrium)以恒定水平(产物/底物＝约20％-35％)存在。因此，制备高纯度的D-阿洛酮糖需要额外的处理，以从酶反应产物中分离和去除相对高浓度的D-果糖。另外，与淀粉或者葡萄糖相比，果糖是一种相对昂贵的原料，当果糖用作原料时，阿洛酮糖和塔格糖的成本率增加。因此，已报道了许多关于使用淀粉或葡萄糖(即相对经济的原料)进行反应，制备阿洛酮糖和塔格糖的研究(KR 10-2018-0004023,WO 2018-112139,WO 2017-059278,WO 2018-129275)。
同时，Chan等人(2008.Biochemistry.47:9608-9617)报道了衍生自酿脓链球菌(Streptococcus pyogenes)的D-核酮糖-5-磷酸-3-差向异构酶(D-ribulose-5-phosphate-3-epimerase,EC 5.1.3.1)和衍生自大肠杆菌(E.coli)的D-阿洛酮糖-6-磷酸-3-差向异构酶(D-psicose-6-phosphate-3-epimerase,EC 5.1.3.-)，其能够催化D-果糖-6-磷酸(D-fructose-6-phosphate)和D-阿洛酮糖-6-磷酸(D-psicose-6-phosphate)的3-差向异构化。然而，由于耐热性较差，这些酶在工业上不适用。
发明内容
在这样的情况下，本申请的发明人认真地进行了研究，以开发一种以经济的方式在工业规模上提高阿洛酮糖的转化率的方法。结果，本申请的发明人发现，将廉价的原料蔗糖或淀粉(例如麦芽糖糊精)转化为阿洛酮糖-6-磷酸之后，本发明使用阿洛酮糖-6-磷酸磷酸酶(psicose-6-phosphate phosphatase)，其对阿洛酮糖-6-磷酸具有特异性，并参与不可逆的反应途径，可以通过一锅法酶转化(one-pot enzymatic conversions)产生阿洛酮糖，并显著提高阿洛酮糖的转化率，所述酶转化使用多种参与阿洛酮糖生产途径的酶。因此，基于这一发现，完成了本发明。与常用的已知的阿洛酮糖-6-磷酸磷酸酶相比(Panoramic view of a superfamily of phosphatases through substrate profiling,PNAS,06.04.2015,Huang,etc.)，本申请中的酶在制备阿洛酮糖方面更有优势。
技术方案
本发明的一个目的是提供一种阿洛酮糖-6-磷酸磷酸酶。
本发明的另一个目的是提供一种编码所述阿洛酮糖-6-磷酸磷酸酶的核酸。
本发明的另一个目的是提供一种转化子，其包含编码阿洛酮糖-6-磷酸磷酸酶的核酸。
本发明的又一个目的是提供一种用于制备阿洛酮糖的组合物，其包含阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物。
本发明的又一个目的是提供一种制备阿洛酮糖的方法，其包括使阿洛酮糖-6-磷酸与阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物接触，以将阿洛酮糖-6-磷酸转化为阿洛酮糖。
有益效果
本发明的新型的酶及其组合是耐热的，因此，本发明的酶可以以工业方式参与将阿洛酮糖-6-磷酸转化为阿洛酮糖的途径，使得以葡萄糖或淀粉(例如麦芽糖糊精)为廉价原料合成阿洛酮糖的途径得以进行，并通过不可逆的反应途径，即阿洛酮糖-6-磷酸的去磷酸化，制备阿洛酮糖，从而显著提高阿洛酮糖的转化率。耐热酶可以经济地防止微生物，并且容易与高浓度底物发生反应，因此其效果显着。另外，通过提高阿洛酮糖的转化率，本发明的制备方法可以简化或消除分离及纯化的步骤(其中包含高浓度的反应产物(即阿洛酮糖))，因而本发明的制备方法具有经济优势，同时制备方法简单。特别地，可以减少或消除使用SMB进行的分离，从而最大限度地提高分离的效率和产量。
具体实施方式
在下文中将详细描述本发明。同时，对于常规元素，本发明公开的一方面和实施方案的描述都可以应用于其他方面和实施方案。此外，本发明公开的各种元素的所有组合都落入本发明的范围内。另外，本发明的范围不受限于以下的具体描述。
为了实现上述的目的，本发明的一方面提供一种阿洛酮糖-6-磷酸磷酸酶。
具体地，本发明的阿洛酮糖-6-磷酸磷酸酶可以为衍生自以下属的阿洛酮糖-6-磷酸磷酸酶：脂环酸芽孢杆菌属(Alicyclobacillus)、拟无枝酸菌属(Amycolatopsis)、厌氧绳菌属(Anaerolinea)、古球菌属(Archaeoglobus)、芽孢杆菌属(Bacillus)、热解纤维素果汁杆菌属(Caldicellulosiruptor)、暖绳菌属(Caldilinea)、暖发菌属(Caldithrix)、一氧化碳胞菌属(Carboxydocella)、一氧化碳嗜热菌属(Carboxydothermus)、绿弯菌门(Chloroflexi)、废水袍菌(Defluviitoga)、异常球菌属(Deinococcus)、除硫球菌属(Desulfurococcus)、网球菌属(Dictyoglomus)、Effusibacillus、闪烁杆菌属(Fervidobacterium)、地芽孢杆菌属(Geobacillus)、盐球菌属(Halococcus)、Hydrogenivirga、氢杆菌属(Hydrogenobacter)、超热菌属(Hyperthermus)、Kosmotoga、Marinitoga、亚栖热菌属(Meiothermus)、Mesotoga、生金球菌属(Metallosphaera)、甲烷胞菌属(Methanocella)、拟甲烷球菌属(Methanococcoides)、甲烷盐菌属(Methanohalobium)、甲烷叶菌属(Methanolobus)、甲烷八叠球菌属(Methanosarcina)、甲烷热菌属(Methanothermus)、石袍菌属(Petrotoga)、嗜酸菌属(Picrophilus)、假诺卡氏菌属(Pseudonocardia)、火球菌属(Pyrococcus)、热网菌属(Pyrodictium)、红嗜热盐菌属(Rhodothermus)、史雷克氏菌属(Slackia)、葡萄嗜热菌属(Staphylothermus)、硫化叶菌属(Sulfolobus)、Thermanaerothrix、Thermoanaerobacter、Thermoanaerobacterium、嗜热裂孢菌属(Thermobifida)、热球菌属(Thermococcus)、Thermocrinis,Thermoflexus、栖热袍菌属(Thermotoga)、栖热菌属(Thermus)以及特吕珀菌属(Truepera)，并且，更具体地，衍生自酸热脂环芽孢杆菌(Alicyclobacillus acidocaldarius)、腾冲脂环酸芽孢杆菌(Alicyclobacillus tengchongensis)、热黄拟无枝酸菌(Amycolatopsis thermoflava)、Anaerolinea thermolimosa、Anaerolinea thermophila、Archaeoglobus fugidus、Archaeoglobus profundus、嗜热硫酸盐还原菌(Archaeoglobus veneficus)、地衣芽孢杆菌(Bacillus licheniformis)、热解纤维素菌(Caldicellulosiruptor bescii)、Caldilinea aerophila、Caldithrix abyssi、Carboxydocella sp.ULO1、Carboxydothermus ferrireducens、绿弯菌门细菌54-19(Chloroflexi bacterium 54-19)、突尼斯废水袍菌(Defluviitoga tunisiensis)、Deinococcus aerius、Deinococcusapachensis、Deinococcus aquatilis、Deinococcus geothermalis、Deinococcushopiensis、Deinococcus maricopensis、Deinococcus murrayi、Deinococcusreticulitermitis、Deinococcus wulumuqiensis、Deinococcus sp.Leaf326、Deinococcusphoenicis、Deinococcus proteolyticus,Deinococcus sp.17bor-2、Deinococcus sp.NW-56,Deinococcus sp.RL、Deinococcus sp.YIM 77859、粘硫还原球菌(Desulfurococcusmucosus)、嗜热网球菌(Dictyoglomus turgidum)、Effusibacillus pohliae、Fervidobacterium gondwanense、Fervidobacterium islandicum、Fervidobacteriumnodosum、Fervidobacterium pennivorans、Geobacillus sp、嗜热脂肪地芽孢杆菌(Geobacillus stearothermophilus)、Halococcus salifodinae、Hydrogenivirgasp.128-5-R1-1、嗜氢产氢杆菌(Hydrogenobacter hydrogenophilus)、嗜氢氢杆菌(Hydrogenobacter thermophilus)、丁酸栖高温菌(Hyperthermus butylicus)、Kosmotogaarenicorallina、Kosmotoga olearia、Marinitoga piezophila、Meiothermus cerbereus、Meiothermus chliarophilus、Meiothermus ruber、Meiothermus Silvanus、Meiothermustaiwanensis、Meiothermus timidus、Meiothermus rufus、Mesotoga infera、Metallosphaera sedula、Methanocella conradii、Methanococcoides methylutens、Methanohalobium evestigatum、Methanolobus tindarius、Methanosarcina sicilia、Methanothermus fervidus、Petrotoga mobilis、Picrophilus torridus、Pseudonocardiathermophila、Pyrococcus furiosus、Pyrodictium occultum、海洋红嗜热盐菌(Rhodothermus marinus)、Slackia heliotrinireducens、海葡萄嗜热菌(Staphylothermus marinus)、嗜酸热硫化叶菌(Sulfolobus acidocaldarius)、Thermanaerothrix daxensis、嗜热厌氧菌(Thermoanaerobacter sp.)、热硫化氢嗜热厌氧杆菌(Thermoanaerobacter thermohydrosulfuricus)、威吉利热厌氧杆菌(Thermoanaerobacter wiegelii)、木聚糖热厌氧杆菌(Thermoanaerobacteriumxylanolyticum),耐盐嗜热双岐菌(Thermobifida halotolerans)、速生热球菌(Thermococcus celer)、Thermococcus litoralis、深嗜热球菌(Thermococcusprofundus)、密涅瓦热发状菌(Thermocrinis minervae)、Thermocrinis ruber、Thermoflexus hugenholtzii、莱廷格热袍菌(Thermotoga lettingae)、那不勒斯栖热袍菌(Thermotoga neapolitana)、嗜石油热袍菌(Thermotoga petrophilia)、Thermusamyloliquefaciens、丝状栖热菌(Thermus filiformis)、嗜热栖热菌(Thermusthermophilus)或Truepera radiovictrix，但不限于此。
如本文所用，已知术语“阿洛酮糖-6-磷酸(psicose-6-phosphate)”为阿洛酮糖-6-磷酸(allulose-6-phosphate)，并且所述“阿洛酮糖-6-磷酸磷酸酶”是指这样的酶，其通过将阿洛酮糖-6-磷酸的磷酸基团去磷酸化，催化阿洛酮糖的产生。
在分解高浓度淀粉制备阿洛酮糖的过程中，可以使用本发明的阿洛酮糖-6-磷酸磷酸酶，与淀粉加工酶和磷酸糖-转化酶结合，以高效地制备阿洛酮糖。
本发明的阿洛酮糖-6-磷酸磷酸酶可以具有SEQ ID NO：1至222中的任一氨基酸序列，或包含与所述氨基酸序列具有至少70％的同源性的氨基酸序列。
另外，其可以具有SEQ ID NO：1、6、9、12、26、29、38至43、45至53、56、57、59、60、64至66、69、70、72、76、80、81、91至93、95、99至103、113、114、116、117、131、134、136、142、145、146、148、164、167、169、172、177、184至187、189、191、192、211、217和221中的任一氨基酸序列，或包含与所述氨基酸序列具有至少70％的同源性的氨基酸序列。
更特别地，其可以具有SEQ ID NO：26、29、53、56、60、70、76、80、81、116、117、131、134、145、167、185、186和191中的任一氨基酸序列，或包含与所述氨基酸序列具有至少70％的同源性的氨基酸序列，但不限于此。
此外，可以包括但不限于具有与上述氨基酸序列相同活性的任何序列。另外，其可以包含SEQ ID NO：1至222中的任一氨基酸序列，或包含与上述氨基酸序列具有至少80％的同源性(homology)或相同性(identity)的氨基酸序列，但不限于此。特别地，所述氨基酸可以包括与上述氨基酸序列具有至少70％、75％、80％、85％、90％、95％、96％、97％、98％或99％同源性或相同性的氨基酸。此外，具有如下的氨基酸序列的任何蛋白显然也可以落入本发明的范围内，只要其包含具有上述同源性或相同性的氨基酸序列，并表现出与上述蛋白相应的功能，其中所述氨基酸序列的部分缺失、修饰、取代或添加。
即，在本发明中，尽管描述为“具有特定SEQ ID NO的氨基酸序列的蛋白”，但是，如下的任何蛋白显然也可以用于本发明，只要所述蛋白具有与相应的SEQ ID NO的氨基酸序列组成的蛋白相同或相应的活性，其中蛋白的氨基酸序列的部分缺失、修饰、取代或添加。例如，只要所述蛋白具有与所述酶相同或相应的活性，就不排除在其氨基酸序列的上游或下游的序列的添加(这不会改变蛋白的功能)、其自然发生的突变、沉默突变或保守取代。即使所述蛋白存在这样的序列添加或突变，其也显然落入本发明的范围内。
如本文所用，术语“同源性”或“相同性”是指两个给定的氨基酸序列或核苷酸序列之间的相关程度，并且可以表示为百分比。
术语“同源性”和“相同性”通常可以互换使用。
保守的多核苷酸或多肽的序列同源性或相同性可以通过标准比对算法来确定，并且可以与所使用的程序所建立的默认空位罚分一起使用。基本上，在中等或高度严格条件下，同源或相同的序列可以杂交，使得序列的全长或全长的至少约50％、60％、70％、80％或90％或更多可以杂交。在杂交的多核苷酸中，还考虑包含简并密码子而非密码子的多核苷酸。
任何两个多核苷酸序列是否具有同源性、相似性或相同性，例如可以通过已知的计算机算法例如使用默认参数的“FASTA”程序(Pearson et al.,(1988)[Proc.Natl.Acad.Sci.USA 85]:2444)来确定。或者，也可以通过Needleman-Wunsch算法(Needleman and Wunsch,1970,J.MolBiol.48:443-453)来确定，其通过EMBOSS软件包的Needle程序运行(EMBOSS:欧洲分子生物学开放软件套件，Rice et al.,2000,TrendsGenet.16:276-277)(优选5.0.0或之后的版本)(GCG程序包)(Devereux,J.et al.,NucleicAcids Research 12:387(1984)),BLASTP,BLASTN,FASTA(Atschul,S.F.et al.,J MOLECBIOL 215:403(1990)；Guide to Huge Computers,Martin J.Bishop,ed.,AcademicPress,San Diego,1994；and CARILLO et al.(1988)SIAM J Applied Math 48:1073)(1988)SIAM J Applied Math48:1073)。例如，同源性、相似性或相同性可以使用美国国家生物技术信息中心(NCBI)的BLAST和ClustalW确定。
多核苷酸或多肽的同源性、相似性或相同性可以通过使用例如GAP计算机程序比对序列信息来确定，例如在Smith and Waterman,Adv.Appl.Math(1981)2:482中公开的Needleman et al.(1970),J Mol Biol.48:443。总之，GAP程序将同源性、相似性或相同性定义为相似比对的符号(即核苷酸或氨基酸)的数目除以两个序列中较短者的符号的总数而获得的值。用于GAP程序的默认参数可以包括(1)一元比较矩阵(其包含的数值对于相同为1，对于不同为0)以及在Schwartz and Dayhoff，eds.,Atlas Of Protein Sequence AndStructure,National Biomedical Research Foundation,pp.353-358(1979)(或EDNAFULL(NCBI NUC4.4的EMBOSS版本)替换矩阵)中描述的Gribskov et al.(Nucl.Acids Res.14:6745(1986))的加权比较矩阵；(2)每个空位罚分是3.0，每个空位的每个符号附加0.10罚分(或空位开位罚分10和空位扩展罚分0.5)；(3)末端空位不罚分。因此，如本文所用，术语“同源性”或“相同性”是指序列之间的相关性(relevance)。
本发明的阿洛酮糖-6-磷酸磷酸酶可以是选择性催化阿洛酮糖-6-磷酸的去磷酸化的酶。具体地，与葡萄糖-1-磷酸(glucose-1-phosphate)、葡萄糖-6-磷酸(glucose-6-phosphate)、或果糖-6-磷酸(fructose-6-phosphate)混合在一起时，阿洛酮糖-6-磷酸磷酸酶可以是催化阿洛酮糖-6-磷酸的去磷酸化的酶。例如，当阿洛酮糖-6-磷酸磷酸酶与等量的阿洛酮糖-6-磷酸、葡萄糖-1-磷酸、葡萄糖-6-磷酸以及果糖-6-磷酸混合时，阿洛酮糖-6-磷酸的去磷酸化效率为1％或更高，10％或跟高，或30％或更高。由于本发明的阿洛酮糖-6-磷酸磷酸酶的选择性活性，在一锅法酶转化中，同时使用多种酶和底物表现出高效率的阿洛酮糖转化。
本发明的阿洛酮糖-6-磷酸磷酸酶可以通过以下制备，用酶本身或表达所述酶的DNA来转化菌株，培养所转化的菌株以获得培养物，并破坏培养物，随后通过柱层析纯化。用于转化的菌株可以是大肠杆菌(Escherichia coli)、谷氨酸棒杆菌(Corynebacterumglutamicum)、米曲霉(Aspergillus oryzae)、酿酒酵母(Saccharomyces cerevisiae)、Yarrowia lipolytica、毕赤酵母(Pichia pastoris)或枯草芽孢杆菌(Bacillussubtilis)，但不限于此，并且其可能有潜力随后被转化为GRAS菌株(通常被认为是安全的)。
本发明的另一个方面提供一种编码阿洛酮糖-6-磷酸磷酸酶的核酸，或包含所述核酸的载体。
如本文所用，术语“核酸”具有共同包括DNA或RNA分子的含义。作为核酸基本结构单元的核苷酸不仅包括天然核苷酸，还包括其中糖或碱基位点被修饰的类似物(参见Scheit,Nucleotide Analogs,John Wiley,New York(1980)；Uhlman and Peyman,Chemical Reviews,90:543-584(1990))。
本发明的编码所述酶的核酸可以是这样的DNA或RNA序列，其中作为单元的核苷酸共价连接，并且特别地，其可以是SEQ ID NO：1至222(将氨基酸修饰为61个密码子)的氨基酸序列在DNA转化时所有可能的数字中的任一核苷酸序列，更特别地，其可以包括一种核酸，所述核酸能够被翻译以表现出所需的酶活性，同时与每个核苷酸具有至少90％或更高、95％或更高、97％或更高、99％或更高或100％同源性、相似性或相同性，所述核苷酸可以被翻译成本发明的SEQ ID NO：1至222中的任一氨基酸序列。显然，由于密码子的简并性，具有相同活性的蛋白、转录后具有相同氨基酸序列的蛋白，特别地，由SEQ ID NO：1至222中的任一氨基酸序列组成的蛋白，或可以翻译成与其具有同源性、相似性或相同性的蛋白的多核苷酸也落入本发明的范围内。更特别地，本发明所述的核酸序列并没有单独提出，其可以包含所有数字的DNA密码子，所述密码子可以被翻译成SEQ ID NO：1至222的氨基酸序列，但不限于此。
另外，可以根据可已知的基因序列制备的探针，例如在严格条件下可以与编码本发明所述的酶的核苷酸序列的全部或部分互补的序列杂交的任何序列，亦可无限制地包括在内。
“严格条件(stringent condition)”是指在该条件下多核苷酸之间可以特异性杂交的条件。这样的条件在文献(J.Sambrook et al.,supra 9.50–9.51,11.7–11.8)中有具体的描述。例如，严格条件可以包括，在该条件下，具有高同源性或相同性的基因，即具有80％或更高，85％或更高，特别地，90％或更高，更特别地，95％或更高，更具体的97％或更高，更特别地99％或更高的同源性或相同性的基因彼此杂交，而同源性或相同性低于上述同源性或相同性的基因彼此不杂交；或者可以包括Southern杂交的清洗条件，即在60℃、1×SSC和0.1％SDS，特别地，60℃、0.1×SSC和0.1％SDS，并且特别地，68℃、0.1×SSC和0.1％SDS的盐浓度和温度下，清洗一次，特别地，清洗两次或三次的条件。
杂交需要两个核酸具有互补序列，即使碱基之间可能错配(mismatch)，这取决于杂交的严格性。术语“互补的”用于描述能够彼此杂交的核苷酸碱基之间的关系。例如，对于DNA，腺嘌呤与胸腺嘧啶互补，胞嘧啶与鸟嘌呤互补。因此，本发明可以包括与整个序列互补的分离的核苷酸片段，以及与其基本相似的核酸序列。
特别地，具有同源性或相同性的多核苷酸可以使用包括在上述条件下的Tm值为55℃的杂交步骤的杂交条件下来检测。另外，Tm值可以是60℃、63℃或65℃，但不限于此，并且本领域技术人员可以根据目的适当地调整。
用于杂交多核苷酸的合适的严格性取决于多核苷酸的长度和互补程度，并且这些变量是本领域众所周知的(参见Sambrook等，同上文，9.50-9.51，11.7-11.8)。
如本文所用，术语“载体”可以指DNA构建体，其包含编码本发明的酶的核酸的核苷酸序列，所述核苷酸序列被可操作性地连接到一个合适的调节序列上，以使经修饰的目标蛋白可以在合适的宿主中表达。所述调节序列可以包含能够启动转录的启动子，任何调节转录的操纵子序列，编码合适的mRNA核糖体结合结构域的序列，以及调节转录和翻译终止的序列。转化至合适的宿主细胞后，所述载体可以复制或者独立于宿主的基因组而发挥作用，并可以整合到宿主的基因组中。
本发明使用的载体没有特别的限制，只要其能够在宿主细胞中复制，并且可以使用本领域已知的任何载体。常用的载体的实例可以包括天然或重组质粒、粘粒、病毒和噬菌体。例如，pWE15、M13、MBL3、MBL4、IXII、ASHII、APII、t10、t11、Charon4A和Charon21A等可用作噬菌体载体或粘粒载体，基于pBR、pUC、pBluescriptII、pGEM、pTZ、pCL和pET等的载体可用作质粒载体。特别地，可以使用pDZ、pACYC177、pACYC184、pCL、pECCG117、pUC19、pBR322、pMW118和pCC1BAC载体。
本发明的又一方面提供一种转化子，其包含具有编码本发明的酶的核酸的载体。
如本文所用，“包含编码所述酶的核酸的转化子”或者“包含具有编码所述酶的核酸的载体的转化子”可以指微生物，其经过重组以表达本发明的阿洛酮糖-6-磷酸磷酸酶。例如，其可以是宿主细胞或微生物，其含有编码阿洛酮糖-6-磷酸磷酸酶的核酸，或其通过含有编码阿洛酮糖-6-磷酸磷酸酶的核酸的载体进行转化，以表达阿洛酮糖-6-磷酸磷酸酶。为了本发明的目的，通过所述转化子表达的阿洛酮糖-6-磷酸磷酸酶可以包含SEQ IDNO:1至222中的任一氨基酸序列，但不限于此。
如本文所用，术语“转化”是指将包含编码阿洛酮糖-6-磷酸磷酸酶的核酸的载体引入到宿主细胞中，以使所述核酸编码的蛋白得以在宿主细胞中表达。转化的核酸是插入并位于宿主细胞的染色体中，或者是位于染色体外，或者是包括上述两种情况，这并不重要，只要其可以在宿主细胞中表达。此外，所述核酸可以包含编码核酸的DNA和RNA，所述DNA和RNA编码本发明的阿洛酮糖-6-磷酸磷酸酶。核酸可以任意形式引入，只要其可以被转入到宿主细胞，并在其中表达。例如，可以以表达盒(expression cassette)的形式将核酸引入宿主细胞，所述表达盒为包含自主表达所需的所有元件的基因构建体。通常，表达盒可包含与核酸可操作地连接的启动子(promoter)、转录终止子、核糖体结合结构域或翻译终止子。表达盒可以是自复制的表达载体的形式。另外，可以将核酸本身引入宿主细胞，并与在宿主细胞中表达所需的序列可操作地连接，但不限于此。
另外，如本文所用，术语“可操作地连接”是指基因序列功能性地连接至启动子序列，所述启动子序列启动和调节核酸的转录，所述核酸编码本发明的阿洛酮糖-6-磷酸磷酸酶。
可以通过本领域已知的任何方法将核酸或载体插入到染色体中，例如，通过同源重组，但所述方法不限于此。另外，载体还可以包含选择标记以确认染色体的插入。选择标记用于选择通过载体转化的细胞，即用于确认目标核酸分子是否已插入；可以使用如下能够提供可选择的表型的标记，例如耐药性、营养缺陷型、对细胞毒剂的抗性，或表面修饰的蛋白的表达。当使用选择性试剂进行治疗时，仅表达选择标记的细胞可以存活或者表达其他表型性状，因此可以选择转化的细胞。
本发明的转化载体的方法可以包括任何能将核酸引入细胞的方法，并且根据宿主细胞，可以选择本领域已知的合适标准技术实施。例如，所述方法可以包括电穿孔、磷酸钙(CaPO4)、氯化钙(CaCl2)沉淀、逆转录病毒感染、显微注射、聚乙二醇(PEG)法、DEAE-葡聚糖法、阳离子脂质体法和醋酸锂-DMSO法，但不限于此。
对于宿主细胞，优选使用DNA引入效率高，并且高效率地表达引入的DNA的宿主细胞。例如，可以是Corynebacterium属微生物、Escherichia属微生物、Serratia属微生物、Bacillus属微生物、Saccharomyces属微生物或Pichia属微生物，并且特别地，可能是E.coli，但不限于此，并且可以应用于所有GRAS菌株。
更特别地，本发明的转化子总共可以有222种，即E.coli BL21(DE3)/pET-CJ-ap1至E.coli BL21(DE3)/pET-CJ-ap222。
本发明的另一方面提供一种用于制备阿洛酮糖的组合物，其包含阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物。特别地，所述组合物还可以包含磷酸糖。更特别地，所述组合物还可以包含阿洛酮糖-6-磷酸作为底物，但不限于此。
本发明的用于制备阿洛酮糖的组合物可以通过包含使阿洛酮糖-6-磷酸去磷酸化的阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物，使阿洛酮糖-6-磷酸去磷酸化以制备阿洛酮糖。
另外，本发明的用于制备阿洛酮糖的组合物还可以包含酶和/或底物[(i)淀粉、麦芽糖糊精、蔗糖或其组合；(ii)磷酸盐(phosphate)或聚磷酸盐(polyphosphate)或其他磷酸化化合物；(iii)果糖-6-磷酸-3-差向异构酶；(iv)葡萄糖-6-磷酸异构酶；(v)磷酸葡萄糖变位酶或葡萄糖激酶；和/或(vi)α-葡聚糖磷酸化酶、淀粉磷酸化酶、麦芽糖糊精磷酸化酶、蔗糖磷酸化酶、α-淀粉酶、支链淀粉酶、异淀粉酶、葡萄糖淀粉酶、α-葡聚糖转移酶、聚磷酸葡萄糖激酶或蔗糖酶]，其参与本发明的阿洛酮糖制备途径葡萄糖淀粉酶聚磷酸葡萄糖激酶；表达参与到阿洛酮糖制备途径的酶的微生物；或微生物的培养物，所述微生物表达参与到阿洛酮糖制备途径的酶。然而，本发明的用于制备阿洛酮糖的组合物中包含的酶，以及用于制备阿洛酮糖的底物仅为说明性，并不限于此，只要可以使用本发明的阿洛酮糖-6-磷酸磷酸酶制备阿洛酮糖。
特别地，所述果糖-6-磷酸-3-差向异构酶可以包含任何在果糖-6-磷酸转化为阿洛酮糖-6-磷酸的过程中具有活性的蛋白。葡萄糖-6-磷酸异构酶可以包含任何在葡萄糖-6-磷酸转化为果糖-6-磷酸的过程中具有活性的蛋白。磷酸葡萄糖变位酶(phosphoglucomutase,EC 5.4.2.2)可以包含任何在葡萄糖-1-磷酸转化为葡萄糖-6-磷酸的过程中具有活性的蛋白。淀粉/麦芽糖糊精磷酸化酶(starch/maltodextrinphosphorylase,EC 2.4.1.1)和α-葡聚糖磷酸化酶可以包含任何在磷酰基转移到葡萄糖，以由淀粉或麦芽糖糊精制备葡萄糖-1-磷酸的过程中具有活性的蛋白。蔗糖磷酸化酶(sucrose phosphorylase,EC 2.4.1.7)可以包含任何在磷酰基转移到葡萄糖，以从蔗糖制备葡萄糖-1-磷酸的过程中具有活性的蛋白。α-淀粉酶(α-amylase,EC 3.2.1.1)、支链淀粉酶(pullulanase,EC 3.2.1.41)、异淀粉酶(isoamylase,EC 3.2.1.68)、4-α-葡聚糖转移酶(4-α-glucanotransferase,EC 2.4.1.25)和葡萄糖淀粉酶(glucoamylase,EC 3.2.1.3)是淀粉液化酶，其可以包含任何在淀粉或麦芽糊精转化为脱支麦芽低聚糖或葡萄糖的过程中具有活性的蛋白。蔗糖酶(sucrase,EC 3.2.1.26)可以包含任何在蔗糖转化为葡萄糖的过程中具有活性的蛋白。聚磷酸葡萄糖激酶(polyphosphate glucokinase,EC 2.7.1.63)可以包含任何在磷酰基转移至葡萄糖以将葡萄糖转化为葡萄糖-6-磷酸的过程中具有活性的蛋白。
本发明的用于制备阿洛酮糖的组合物包括阿洛酮糖-6-磷酸磷酸酶、α-葡聚糖磷酸化酶、磷酸葡萄糖变位酶(或磷酸甘露糖变位酶)、葡萄糖-6-磷酸异构酶、阿洛酮糖-6-磷酸-3-差向异构酶(或核酮糖-5-磷酸-3-差向异构酶)、支链淀粉酶(或异淀粉酶)、4-α-葡聚糖转移酶和聚磷酸葡萄糖激酶，其与最终产物阿洛酮糖几乎没有或没有副反应。
本发明的用于制备阿洛酮糖的组合物不仅可以适当地含有阿洛酮糖-6-磷酸磷酸酶，还可以适当地含有用于制备阿洛酮糖的多种酶及其底物。阿洛酮糖-6-磷酸磷酸酶具有如下的作用，即使处于存在多种酶的环境中，也能够由阿洛酮糖-6-磷酸选择性地且不可逆地制备阿洛酮糖。
本发明的用于制备阿洛酮糖的组合物还可以包含任何合适的常规使用的赋形剂。这样的赋形剂的实例包括，例如，防腐剂、润湿剂、分散剂、悬浮剂、缓冲剂、稳定剂和等渗剂，但不限于此。
本发明的用于制备阿洛酮糖的组合物可以进一步包含金属离子或金属盐。在一实施方案中，金属离子可以是二价金属阳离子。特别地，金属离子可以为选自以下的至少一种：Ni、Mg、Co、Mn、Fe和Zn离子。更特别地，本发明用于制备塔格糖的组合物可以进一步包含金属盐。更特别地，金属盐可以为选自以下的至少一种：NiSO4、MgSO4、MgCl2、NiCl2、CoSO4、CoCl2、MnCl2、MnSO4、FeSO4和ZnSO4。
本发明的另一个方面提供了一种制备阿洛酮糖的方法，其包括以下步骤，通过使阿洛酮糖-6-磷酸与阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物接触，以将阿洛酮糖-6-磷酸转化为阿洛酮糖。
特别地，在制备阿洛酮糖的方法中，可以通过以下方法制备阿洛酮糖，使阿洛酮糖-6-磷酸与阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物接触，但不限于此。
本发明的方法还可以包括在将阿洛酮糖-6-磷酸转化为阿洛酮糖之前，通过使果糖-6-磷酸与果糖-6-磷酸-3-差向异构酶、表达果糖-6-磷酸-3-差向异构酶的微生物或表达果糖-6-磷酸-3-差向异构酶的微生物的培养物接触，以将果糖-6-磷酸转化为阿洛酮糖-6-磷酸。
本发明的方法还可以包括在将果糖-6-磷酸转化为阿洛酮糖-6-磷酸之前，通过使葡萄糖-6-磷酸与葡萄糖-6-磷酸异构酶、表达葡萄糖-6-磷酸异构酶的微生物或表达葡萄糖-6-磷酸异构酶的微生物的培养物接触，以将葡萄糖-6-磷酸转化为果糖-6-磷酸。
本发明的方法还可以包括在将葡萄糖-6-磷酸转化为果糖-6-磷酸之前，通过使葡萄糖-1-磷酸与磷酸葡萄糖变位酶、表达磷酸葡萄糖变位酶的微生物或表达磷酸葡萄糖变位酶的微生物的培养物接触，以将葡萄糖-1-磷酸转化为葡萄糖-6-磷酸。
本发明的方法还可以包括在将葡萄糖-6-磷酸转化为果糖-6-磷酸之前，通过使葡萄糖与聚磷酸葡萄糖激酶、表达聚磷酸葡萄糖激酶的微生物或表达聚磷酸葡萄糖激酶的微生物的培养物以及聚磷酸盐接触，以将葡萄糖转化为葡萄糖-6-磷酸。
本发明的方法还可以包括在将葡萄糖-1-磷酸转化为葡萄糖-6-磷酸之前，通过使淀粉、麦芽糊精、蔗糖或其组合与α-葡聚糖磷酸化酶、淀粉磷酸化酶、麦芽糊精磷酸化酶或蔗糖磷酸化酶；表达所述磷酸化酶的微生物或表达所述磷酸化酶的微生物的培养物以及磷酸盐接触，以将淀粉、麦芽糊精、蔗糖或其组合转化为葡萄糖-1-磷酸。
本发明的方法还可以包括在将淀粉、麦芽糖糊精、蔗糖或其组合转化为葡萄糖-1-磷酸之前，通过使淀粉、麦芽糊精、蔗糖或其组合与α-淀粉酶、支链淀粉酶、异淀粉酶、葡萄糖淀粉酶或蔗糖酶；表达所述α-淀粉酶、支链淀粉酶、异淀粉酶、葡萄糖淀粉酶或蔗糖酶的微生物或表达所述α-淀粉酶、支链淀粉酶、葡萄糖淀粉酶、蔗糖酶或异淀粉酶的微生物的培养物接触，以将淀粉、麦芽糊精、蔗糖或其组合转化为葡萄糖。
本发明的制备阿洛酮糖的方法中使用的阿洛酮糖-6-磷酸磷酸酶、α-葡聚糖磷酸化酶、磷酸葡萄糖变位酶(或磷酸甘露糖变位酶)、葡萄糖-6-磷酸异构酶、阿洛酮糖-6-磷酸-3-差向异构酶(或核酮糖-5-磷酸-3-差向异构酶)、支链淀粉酶(或异淀粉酶)、4-α-葡聚糖转移酶以及聚磷酸葡萄糖激酶，与最终产物阿洛酮糖几乎没有或没有副反应。
在发明的制备阿洛酮糖的方法中，可以通过在磷酸糖-转化酶的复杂组合物中分解高浓度的淀粉，制备最优/最大量的阿洛酮糖，并且可以最多使用8种酶，以确保阿洛酮糖的最大产量。
首先，降解淀粉并产生葡萄糖-1-磷酸的酶，即葡聚糖磷酸化酶(糖原磷酸化酶)(glucan phosphorylase,glycogen phosphorylase,EC 2.4.1.1)，利用α-1,4-淀粉特异性地制备葡萄糖-1-磷酸。其次，在中间复合酶反应中，使用磷酸葡萄糖变位酶(phosphoglucomutase,EC 2.7.5.1)或磷酸甘露糖变位酶(phosphomannomutase,EC5.4.2.8)，将由此产生的葡萄糖-1-磷酸转化为葡萄糖-6-磷酸。使用的第三种酶，即葡萄糖-6-磷酸异构酶(glucose-6-phosphate isomerase,EC 5.3.1.9)，将葡萄糖-6-磷酸转化为果糖-6-磷酸。第四，使用核酮糖-5-磷酸-3-差向异构酶或阿洛酮糖-6-磷酸-3-差向异构酶，即如上所述将果糖-6-磷酸转化为阿洛酮糖-6-磷酸的酶，在可逆反应中制备阿洛酮糖-6-磷酸。在转化淀粉至阿洛酮糖-6-磷酸的反应中，虽然在可逆反应中的制备不能够超过一定的量，但使用本发明的选择性阿洛酮糖-6-磷酸磷酸酶能够实现阿洛酮糖的高产量。
为进一步提高淀粉利用率，一起使用支链淀粉酶(EC 3.2.1.41)或异淀粉酶(EC3.2.1.68)，除了支链淀粉的α-1,4键，还降解支链淀粉的支化的α-1,6键。此外，为了提高葡聚糖磷酸化酶的淀粉利用率，使用4-α-葡聚糖转移酶(EC 2.4.1.25)。通过将α-1,4键形式的低聚糖与麦芽糖或其他低聚糖(活性相对较低的底物)结合，可提升分段淀粉底物的利用率。此外，通过复杂酶促反应，聚磷酸-葡萄糖磷酸转移酶(EC 2.7.1.63)可用于由淀粉使用后降解的葡萄糖制备额外的阿洛酮糖，从而确保最大的阿洛酮糖转化率。
此外，在发明的方法中，所述接触可以在5.0至9.0的pH下进行，特别地，在6.0至8.0的pH下进行。
在本发明的方法中，所述接触可以在40℃至80℃的温度下进行，特别地，在40℃至60℃或50℃至60℃的温度下进行。
在本发明的方法中，所述接触可以进行2小时至24小时，特别地，6至24小时或120小时。
在本发明的方法中，所述接触可以在5.0至9.0的pH，40℃至80℃的温度下，和/或进行2小时至24小时。特别地，所述接触可以在6.0至8.0的pH，40℃至60℃或50℃至60℃的温度下，进行6小时至24小时或120小时。
本发明的方法还可以包括纯化阿洛酮糖。对于纯化阿洛酮糖的方法没有特别的限制，可以使用本领域已知的任何合适的方法。所述纯化方法的非限制性实例包括色谱法、分步结晶法和离子纯化法，可以单独实施或以其两种或更多种的组合实施。例如，可以通过色谱法纯化反应产物阿洛酮糖。在这种情况下，通过色谱法纯化糖类可以基于需要纯化的糖类与附着在离子树脂上的金属离子之间的结合力的微小差异来进行。
本发明所述方法还可包括在纯化步骤之前或之后的漂白、去矿化，或两者皆有。所述漂白和/或去矿化可以制备更纯的阿洛酮糖，并且不含杂质。
实施例
以下将通过实施例更详细地描述本发明。然而，仅出于示例性目的以帮助理解本发明而提供这些实施例，并且本发明的范围不意图受这些实施例所限制。
在本发明中，氨基酸可以表示为以下缩写或氨基酸名称。
表1
氨基酸类型 缩写 编码对应氨基酸的DNA密码子 编码对应氨基酸的RNA密码子
丙氨酸 A GCT,GCC,GCA,GCG GCU,GCC,GCA,GCG
精氨酸 R AGA,AGG AGA,AGG
天冬酰胺 N AAT,AAC AAU,AAC
天冬氨酸 D GAT,GAC GAU,GAC
半胱氨酸 C TGT,TGC UGU,UGC
谷氨酸 E GAA,GAG GAA,GAG
谷氨酰胺 Q CAA,CAG CAA,CAG
甘氨酸 G GGT,GGC,GGA,GGG GGU,GGC,GGA,GGG
组氨酸 H CAC,CAT CAC,CAU
异亮氨酸 I ATT,ATC,ATA AUU,AUC,AUA
亮氨酸 L TTA,TTG,CTT,CTC,CTA,CTG UUA,UUG,CUU,CUC,CUA,CUG
赖氨酸 K AAA,AAG AAA,AAG
蛋氨酸 M ATG AUG
苯丙氨酸 F TTT,TTC UUU,UUC
脯氨酸 P CCT,CCC,CCA,CCG CCU,CCC,CCA,CCG
丝氨酸 S TCT,TCC,TCA,TCG UCU,UCC,UCA,UCG
苏氨酸 T ACT,ACC,ACA,ACG ACU,ACC,ACA,ACG
色氨酸 W TGG UGG
酪氨酸 Y TAT,TAC UAU,UAC
缬氨酸 V GTT,GTC,GTA,GTG GUU,GUC,GUA,GUG
实施例1：每种酶和转化的微生物的重组表达载体的制备
为了提供用于制备阿洛酮糖的途径的必需的酶，选择耐热性基因，如下表2所示。
表2
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使用每个培养菌株的基因合成或染色体DNA(基因组DNA)，通过聚合酶链反应(PCR)，对所选择氨基酸的基因进行扩增，并使用限制酶NdeI以及XhoI或SalI，将扩增的DNA插入质粒载体pET2la(Novagen)，以构建重组表达载体，所述质粒载体pET2la用于大肠杆菌表达。使用通用转化技术(参见Sambrook et al.1989)将表达载体转化到大肠杆菌BL21(DE3)菌株中，以制备的转化的微生物。
特别地，所述SEQ ID NO:26、29、53、56、60、70、76、80、81、116、117、131、134、145、167、185、186和191的阿洛酮糖-6-磷酸磷酸酶被转化到大肠杆菌BL21(DE3)菌株中，以制备转化的微生物，其被命名为E.coli BL21(DE3)/pET-CJ-ap26、E.coli BL21(DE3)/pET-CJ-ap29、E.coli BL21(DE3)/pET-CJ-ap53、E.coli BL21(DE3)/pET-CJ-ap56、E.coli BL21(DE3)/pET-CJ-ap60、E.coliBL21(DE3)/pET-CJ-ap70、E.coli BL21(DE3)/pET-CJ-ap76、E.coli BL21(DE3)/pET-CJ-ap80、E.coli BL21(DE3)/pET-CJ-ap81、E.coli BL21(DE3)/pET-CJ-ap116、E.coli BL21(DE3)/pET-CJ-ap117、E.coli BL21(DE3)/pET-CJ-ap131、E.coli BL21(DE3)/pET-CJ-ap134、E.coli BL21(DE3)/pET-CJ-ap145、E.coli BL21(DE3)/pET-CJ-ap167、E.coli BL21(DE3)/pET-CJ-ap185、E.coli BL21(DE3)/pET-CJ-ap186以及E.coli BL21(DE3)/pET-CJ-ap191。
如此制备的转化的微生物于2018年11月14日被保藏于国际保藏机构韩国微生物保藏中心(KCCM)，保藏号为KCCM12390P(E.coli BL21(DE3)/pET-CJ-ap26)、KCCM12391P(E.coli BL21(DE3)/pET-CJ-ap29)、KCCM12392P(E.coli BL21(DE3)/pET-CJ-ap53)、KCCM12393P(E.coli BL21(DE3)/pET-CJ-ap56)、KCCM12394P(E.coli BL21(DE3)/pET-CJ-ap60)、KCCM12395P(E.coli BL21(DE3)/pET-CJ-ap70)、KCCM12396P(E.coli BL21(DE3)/pET-CJ-ap76)、KCCM12397P(E.coli BL21(DE3)/pET-CJ-ap80)、KCCM12398P(E.coli BL21(DE3)/pET-CJ-ap81)、KCCM12399P(E.coli BL21(DE3)/pET-CJ-ap116)、KCCM12400P(E.coli BL21(DE3)/pET-CJ-ap117)、KCCM12401P(E.coli BL21(DE3)/pET-CJ-ap131)、KCCM12402P(E.coli BL21(DE3)/pET-CJ-ap134)、KCCM12403P(E.coli BL21(DE3)/pET-CJ-ap145)、KCCM12404P(E.coli BL21(DE3)/pET-CJ-ap167)、KCCM12405P(E.coli BL21(DE3)/pET-CJ-ap185)、KCCM12406P(E.coli BL21(DE3)/pET-CJ-ap186)以及KCCM12407P(E.coliBL21(DE3)/pET-CJ-ap191)。
实施例2：重组酶的制备
为了制备重组酶，将实施例1中制备的每种转化微生物接种到含有5mL LB液体培养基的培养管中。在37℃的振荡培养箱中进行种培养，直至在600nm的吸光度达到2.0。将通过种培养获得的培养液接种在含有LB液体培养基的培养瓶中，然后进行主培养。当培养物在600nm的吸光度达到2.0时，加入1mM IPTG以诱导重组酶的表达和制备。在180rpm的搅拌速度和37℃的条件下进行培养。所述培养液在8000xg以及4℃下离心20分钟，以回收细胞。回收的细胞用50mM Tris-HCl(pH 8.0)缓冲液洗涤两次，并在同样的缓冲液中重悬，使用超声均质器破碎所述细胞。所述细胞裂解液在13000xg以及4℃下离心20分钟，以收取上清液。通过His-tag亲和层析，从上清液中纯化所述重组酶，用50mM Tris-HCl缓冲液(pH 8.0)透析所述纯化后的重组酶，并用于接下来的反应。
实施例3：磷酸糖和阿洛酮糖-6-磷酸的脱磷酸活性分析
以果糖-6-磷酸制备阿洛酮糖-6-磷酸后，确定通过阿洛酮糖-6-磷酸磷酸酶制备阿洛酮糖的活性，或者通过进一步加入三种酶，使用葡萄糖-1-磷酸为第一底物，通过检测阿洛酮糖来确定所述活性。特别地，对葡萄糖、果糖和阿洛酮糖进行定性和定量评估，其为通过将本发明的222种磷酸酶中的每一种与三种酶(即磷酸葡萄糖变位酶(EC 2.7.5.1)或磷酸甘露糖变位酶(EC 5.4.2.8)，其将葡萄糖-1-磷酸转化为葡萄糖-6-磷酸；葡萄糖-6-磷酸异构酶(EC 5.3.1.9)，其将葡萄糖-6-磷酸转化为果糖-6-磷酸；以及核酮糖-5-磷酸-3-差向异构酶或阿洛酮糖-6-磷酸-3-差向异构酶，其将果糖-6-磷酸转化为阿洛酮糖-6-磷酸)混合制备而成的常见的糖类。
特别地，将50mM果糖-6-磷酸或20mM葡萄糖-1-磷酸悬浮于50mM Tris-HCl(pH7.0)、50mM磷酸钠(pH 6至7)或50mM磷酸钾(pH 6至7)中，然后向其中加入0.1单位/mL的磷酸葡萄糖变位酶；磷酸甘露糖变位酶、葡萄糖-6-磷酸异构酶和核酮糖-5-磷酸-3-差向异构酶；或向其中加入阿洛酮糖-6-磷酸-3-差向异构酶以及实施例2中制备的222种重组阿洛酮糖-6-磷酸磷酸酶的每一种，混合物在45℃至70℃下反应1至24小时。使用SP 0810色谱柱(Shodex)以及Aminex HPX-87C色谱柱(Bio-RAD)，在80℃以及0.6mL/min的流动相流速下，使用折光率检测器(RID)进行检测，通过HPLC确认葡萄糖、果糖和阿洛酮糖的产生。
之后，在制备的葡萄糖-1-磷酸、葡萄糖-6-磷酸、果糖-6-磷酸和阿洛酮糖-6-磷酸的混合溶液中，测定本发明的阿洛酮糖-6-磷酸磷酸酶的去磷酸化活性以及阿洛酮糖-6-磷酸的特异性(选择性)去磷酸化率。
特别地，将0.1单位/mL的每种阿洛酮糖-6-磷酸磷酸酶以及5mM MgCl2(或MgSO4)加入到1％(w/v)葡萄糖-6-磷酸、葡萄糖-1-磷酸、果糖-6-磷酸和阿洛酮糖-6-磷酸的混合溶液中，混合物在50℃下反应12小时。使用Aminex HPX-87C柱(Bio-RAD)，在80℃下以0.6mL/min的流动相流速，通过HPLC分析反应产物。用折光率检测器检测阿洛酮糖和其他糖类(果糖和葡萄糖)的产生。
结果，在所有222种酶中，69种酶对阿洛酮糖-6-磷酸显示出高选择性的去磷酸化活性，45种酶对阿洛酮糖-6-磷酸显示出弱选择性的去磷酸化活性。此外，在108种酶中，无法确认选择性阿洛酮糖-6-磷酸的去磷酸化效价。然而，除去222种酶中的一种，证实了221种酶的去磷酸化活性，其中113种酶表现出高去磷酸化活性，108种酶表现出低去磷酸化活性。同时，具有SEQ ID NO：54序列的酶被证实没有去磷酸化效价，其为已知的去磷酸化酶，但却被证实没有实质性的去磷酸化活性。结果如下表3总结。
A＝低活性(由磷酸糖到单糖的去磷酸转化率为1-30％)
B＝高活性(由磷酸糖到单糖的去磷酸转化率为30％或更高)
C＝无活性(由磷酸糖到单糖的去磷酸转化率小于1％)
表3
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实施例4：通过多重酶促反应(多重酶)分析产生阿洛酮糖的活性
为了由麦芽糖糊精制备阿洛酮糖，选择了七种酶并同时反应(一锅法)。七种酶如下，葡聚糖磷酸化酶，其降解淀粉并将其转化为葡萄糖-1-磷酸，支链淀粉酶，其使淀粉脱支，4-α-葡聚糖转移酶，其增加淀粉底物的利用率，磷酸葡萄糖变位酶，其将葡萄糖-1-磷酸转化为葡萄糖-6-磷酸，葡萄糖-6-磷酸异构酶，其将葡萄糖-6-磷酸转化为果糖-6-磷酸，阿洛酮糖-6-磷酸-3-差向异构酶，其将果糖-6-磷酸转化为阿洛酮糖-6-磷酸，以及SEQ IDNO：1至222的酶，其能够由阿洛酮糖-6-磷酸制备阿洛酮糖，制备上述七种酶，并以0.1单位/mL的量使用。将5％(w/v)麦芽糖糊精加入1mM至5mM MgCl2以及10mM至50mM磷酸钠(pH 7.0)中，并使混合物在50℃的温度下反应12小时。使用Aminex HPX-87C柱(Bio-RAD)，在80℃下以0.6mL/min的流动相流速，通过HPLC分析反应产物，并使用折光率检测器检测。
结果，通过多重酶促反应证实了有麦芽糖糊精制备了阿洛酮糖。
虽然已经参考特定的说明性实施例描述了本申请，但是本申请所属领域的技术人员将理解，在不脱离本发明技术精神或本质的情况下，本发明可以以其他特定形式实施。因此，应当认为所描述的实施方案在所有方面都仅仅是示例性的而非限制性的。此外，本发明的范围由所附的权利要求而非前文的具体描述指明。落入权利要求的等同物的含义和范围内的所有修改或变化都将包含在本发明的范围内。
[保藏号]
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12390P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12391P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12392P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12393P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12394P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12395P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12396P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12397P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12398P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12399P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12400P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12401P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12402P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12403P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12404P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12405P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12406P
保藏日期：2018年11月14日
保藏单位：KCCM韩国微生物保藏中心
登记号：KCCM12407P
保藏日期：2018年11月14日
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<110> CJ第一制糖株式会社
<120> 新阿洛酮糖-6-磷酸酶，包含其制备阿洛酮糖的组合物，和用其制备阿洛酮糖的方法
<130> OPA19273
<150> KR10-2018-0159351
<151> 2018-12-11
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Asp Trp Thr Ser Ser Lys Asp Val Glu Ala Thr Lys Pro Glu Pro Asp
130 135 140
Leu Leu Glu Val Ala Leu Glu Lys Val Arg Gly Glu Glu Ala Val Val
145 150 155 160
Ile Gly Asp Ser Val Trp Asp Cys Val Ala Ala Arg Arg Ile Gly Leu
165 170 175
Pro Cys Val Gly Leu Leu Thr Gly Gly Phe Ser Ala Ala Glu Leu Thr
180 185 190
Asp Ala Gly Ala Ala Arg Thr Phe Thr Asp Leu Arg Glu Leu Arg Ala
195 200 205
Ser Leu Asp Glu Leu Pro Phe Gly Ala Ser Pro Arg
210 215 220
<210> 6
<211> 406
<212> PRT
<213> Anaerolinea thermolimosa
<400> 6
Met Val Lys Arg Glu Val Leu Ala Ile Ile Pro Ala Arg Gly Gly Ser
1 5 10 15
Lys Gly Ile Pro Arg Lys Asn Val Arg Leu Phe Ala Gly His Pro Leu
20 25 30
Ile Ala Tyr Ser Ile Ala Ala Ala Arg Gln Ser Glu Leu Val Thr Arg
35 40 45
Val Ile Val Ser Thr Asp Asp Glu Glu Ile Ala Arg Val Ala Arg Asp
50 55 60
Tyr Gly Ala Glu Thr Pro Phe Leu Arg Pro Ala Glu Leu Ala Gln Asp
65 70 75 80
Gln Thr Leu Asp Leu Pro Val Phe Gln His Ala Leu Arg Trp Leu Glu
85 90 95
Glu Gln Glu Gly Tyr Gln Pro Glu Leu Val Val Gln Leu Arg Pro Thr
100 105 110
Ser Pro Val Arg Pro Pro Ala Leu Val Asp Glu Ala Val Gln Ile Leu
115 120 125
Leu Ala His Pro Glu Ala His Ser Val Arg Gly Val Val Pro Ala Gly
130 135 140
Gln Asn Pro His Lys Met Trp Arg Ile Asp Pro Gln Asn Gly Gln Met
145 150 155 160
Ile Pro Leu Leu Gln Val Pro Gly Leu Glu Glu Pro Tyr Asn Ala Pro
165 170 175
Arg Gln Val Leu Pro Pro Val Tyr Trp Gln Thr Gly His Ile Asp Val
180 185 190
Ile Arg Pro Glu Val Ile Arg Lys Gly Ser Met Ser Gly Gln Val Ile
195 200 205
Leu Pro Val Met Val Asp Pro Ala Tyr Thr Val Asp Ile Asp Thr Pro
210 215 220
Arg Asp Trp Ala Arg Ser Glu Trp Leu Val Trp Tyr Ser Glu Leu Glu
225 230 235 240
Ile Val Tyr Pro Gly His Arg Arg Arg Pro Leu Pro Glu Lys Val Asp
245 250 255
Leu Val Val Phe Asp Phe Asp Gly Val Leu Thr Asp Asn Arg Val Trp
260 265 270
Val Asp Glu Glu Gly His Glu Met Val Ala Ala Asn Arg Ser Asp Ser
275 280 285
Leu Gly Val Ala Tyr Leu Arg Arg Ser Gly Leu Lys Met Val Val Leu
290 295 300
Ser Thr Glu Thr Asn Pro Val Val Ala Ala Arg Cys Arg Lys Met Gln
305 310 315 320
Ile Pro Val Val Gln Gly Val Trp Asp Lys Ala Gly Glu Leu Pro Arg
325 330 335
Ile Met Ala Glu Phe Asn Ala Ala Pro Glu Arg Thr Ile Tyr Val Gly
340 345 350
Asn Asp Val Asn Asp Val Pro Cys Phe Ser Leu Val Gly Cys Ala Val
355 360 365
Ala Val Ala Asp Ala Gln Pro Ala Ala Arg Arg Ala Ala Asp Ile Val
370 375 380
Leu Ser Arg Asn Gly Gly His Gly Ala Val Arg Glu Leu Leu Asp Ile
385 390 395 400
Leu Met Gln Arg Met Lys
405
<210> 7
<211> 226
<212> PRT
<213> Anaerolinea thermolimosa
<400> 7
Met Gly Ile Asp Arg Gln Arg Val Cys Gly Ile Cys Phe Asp Val Asp
1 5 10 15
Gly Thr Leu Ser Asp Thr Asp Asp Leu Trp Val Ala Arg Leu Ser Gly
20 25 30
Trp Leu Lys Pro Val Ser Trp Ile Phe Pro Gln Arg Asn Pro Ser Arg
35 40 45
Phe Ala Arg Trp Ala Ile Met Ala Ala Glu Ser Pro Gly Asn Leu Val
50 55 60
Tyr Ser Leu Phe Asp Arg Phe Gly Leu Asp Asp Glu Ile Gly Arg Leu
65 70 75 80
Phe His Trp Leu Asn Arg Gln Arg Gly Val Leu Arg Ser Gln Arg Phe
85 90 95
Leu Leu Ile Pro Gly Val Gln Glu Ala Leu His Arg Leu His Lys His
100 105 110
Phe Pro Leu Ala Val Val Ser Ala Arg Asp His Ala Gly Thr Leu Ala
115 120 125
Phe Leu Gln His Phe Glu Leu Glu Pro Phe Phe Arg Cys Val Ala Thr
130 135 140
Ser Gln Thr Cys Glu Phe Thr Lys Pro Phe Pro His Pro Ile Arg Trp
145 150 155 160
Ala Ala Ala Gln Met Gly Val Lys Pro Glu Glu Leu Leu Met Val Gly
165 170 175
Asp Thr Val Val Asp Ile Arg Ala Gly Lys Ala Ala Gly Ala Gln Thr
180 185 190
Val Gly Val Leu Cys Gly Phe Gly Gln Glu Glu Glu Leu Arg Arg Ala
195 200 205
Gly Ala Asp Leu Ile Leu Pro His Thr Ala Gln Leu Ala Asp Val Leu
210 215 220
Leu Asp
225
<210> 8
<211> 222
<212> PRT
<213> Anaerolinea thermolimosa
<400> 8
Met His Phe Ser Ala Tyr Ile Phe Asp Met Asp Gly Thr Leu Ile Asp
1 5 10 15
Asn Met Gly Phe His Gly Ala Ile Trp Ser Glu Phe Leu Ala Ser Leu
20 25 30
Gly Ala Pro Val Asp Arg Glu Thr Phe Phe Arg Arg Thr Val Gly Lys
35 40 45
Val Asn Ala Glu Ile Leu Arg Asp Leu Tyr Arg Ala Asp Leu Ser Asp
50 55 60
Ala Glu Ile Glu Glu Leu Ser Arg Arg Lys Glu Gln Leu Tyr Arg Gln
65 70 75 80
Arg Phe Ala Pro Leu Ile Thr Gln Lys Ala Val Pro Gly Val Arg Glu
85 90 95
Phe Leu Gln Lys Ala His Gln Arg Gly Ile Ser Met Ala Val Ala Thr
100 105 110
Ser Ala Gly Leu Glu Asn Thr Arg Phe Ile Leu Asp Gly Leu Gly Ile
115 120 125
Thr Ala Tyr Phe Ser Ala Leu Val Thr Ser Glu Asp Val His Arg Gly
130 135 140
Lys Pro Asp Pro Glu Ile Phe Leu Ile Ala Ala Gln Lys Leu Gly Val
145 150 155 160
Pro Pro Glu Ala Cys Leu Val Phe Glu Asp Ser Pro Ala Gly Leu Glu
165 170 175
Ala Ala His Arg Ala Gly Met Val Ser Val Ala Leu Ala Thr Thr Phe
180 185 190
Pro Ala Glu Gln Leu Ala Gly Arg Pro Gly Val Leu Leu Val Thr Gly
195 200 205
Asp Tyr Ser Ala Ile Asp Leu Thr Arg Leu Ile Val Gly Thr
210 215 220
<210> 9
<211> 259
<212> PRT
<213> Anaerolinea thermophila
<400> 9
Met Thr Thr Ser Leu Arg Asp Leu Leu Asp Phe Ala Val Glu Thr Ala
1 5 10 15
Tyr Leu Ala Gly Arg Thr Thr Leu Ala Tyr Phe Gln Thr Gly Val Gln
20 25 30
Ala Glu Phe Lys Ala Asp Ser Ser Pro Val Thr Leu Ala Asp Arg Ala
35 40 45
Ala Glu Gln Leu Ile Arg Gln Arg Ile Glu Lys Arg Phe Pro His His
50 55 60
Ala Ile Val Gly Glu Glu Phe Gly Val Gln Gly Ser Ala Asp Ala Thr
65 70 75 80
His Arg Trp Phe Ile Asp Pro Ile Asp Gly Thr Lys Ser Phe Leu Arg
85 90 95
Gly Ile Pro Leu Tyr Ala Val Leu Leu Gly Leu Glu Ile Glu Gly Arg
100 105 110
Val Gln Val Gly Val Ala Tyr Tyr Pro Ala Met Asp Glu Met Leu Ser
115 120 125
Ala Ala Asp Gly Glu Gly Cys Trp Trp Asn Gly Arg Arg Ala Arg Val
130 135 140
Ser Thr Ala Ser Arg Leu Ala Glu Ala Trp Val Thr Ser Thr Asp Pro
145 150 155 160
Tyr Asn Phe Gln Lys Thr Gly Lys Asp Ala Ala Trp Gln Arg Ile Gln
165 170 175
Ala Val Ser Tyr His Arg Gly Gly Trp Gly Asp Ala Tyr Gly Tyr Leu
180 185 190
Leu Val Ala Thr Gly Arg Ala Glu Val Met Leu Asp Pro Ile Met Asn
195 200 205
Glu Trp Asp Cys Ala Pro Phe Pro Pro Ile Phe Arg Glu Ala Gly Gly
210 215 220
Phe Phe Gly Asp Trp Gln Gly Asn Glu Thr Ile Tyr Gly Gly Glu Ala
225 230 235 240
Leu Ala Thr Thr Gln Val Leu Leu Pro Glu Val Leu Glu Cys Leu His
245 250 255
Ser Ser Leu
<210> 10
<211> 223
<212> PRT
<213> Anaerolinea thermophila
<400> 10
Met Thr Lys Thr Asn Ile Lys Gly Ile Ile Phe Asp Phe Asp Gly Leu
1 5 10 15
Ile Leu Asp Thr Glu Thr Pro Glu Met Leu Ala Trp Glu Lys Ala Phe
20 25 30
Thr Glu His Ser Leu Ser Phe Pro Ile Glu Arg Tyr Leu Asn Ser Ile
35 40 45
Gly Thr Val Ser Asp Asn His Phe Val Gln Gly Phe Met Gln Glu Met
50 55 60
Gly Leu Ser Glu Gly Glu Ile Gln Gln Thr Val Leu Ala Tyr Gln Ser
65 70 75 80
His Ile Ala Thr Phe Glu His Met Asn His Pro Arg Glu Gly Val Leu
85 90 95
Asp Leu Ile His Asp Ala Glu Lys Ile Gly Leu Arg Leu Ala Val Ala
100 105 110
Ser Asn Ser Tyr Arg Thr Trp Val Val Asp Asn Leu Gln Arg Leu Ala
115 120 125
Leu Asp Ser His Phe Asp Pro Ile Cys Thr Arg Asp Asp Val Thr Asn
130 135 140
Ser Lys Pro Asp Pro Glu Leu Tyr Asn Leu Val Leu Ala Lys Trp Gly
145 150 155 160
Phe Thr Ser Asn Glu Val Leu Ala Leu Glu Asp Ser Pro Asn Gly Ile
165 170 175
Lys Ala Ala Lys Asn Ala Gly Ile Phe Cys Val Ala Val Pro Asn Pro
180 185 190
Ile Thr Ile Arg Met Asp Val Thr Leu Ala Asp Leu Ile Phe Ser Ser
195 200 205
Phe Ser Asp Phe Ser Leu Gln Glu Ile Leu Phe Gln Leu Glu Lys
210 215 220
<210> 11
<211> 232
<212> PRT
<213> Anaerolinea thermophila
<400> 11
Met Pro Leu Asp Val Ser Arg Ile Gln Ala Ile Cys Phe Asp Ile Asp
1 5 10 15
Gly Thr Leu Ser Asp Thr Asp Asp Glu Trp Ala Glu Arg Val Leu Lys
20 25 30
Gly Ile Ser Phe Leu Lys Gly Trp Val Gly Glu Gln Arg Leu Arg Asn
35 40 45
Leu Ala Arg Trp Val Val Met Glu Ile Glu Ser Pro Gly Asn Leu Leu
50 55 60
Tyr Asn Leu Leu Asp Arg Leu His Leu Asp Asp Glu Ala Ser Arg Leu
65 70 75 80
Phe Asn Phe Phe Val Arg Lys Phe His Pro Arg Pro Ser Arg Phe Arg
85 90 95
Leu Ile Ser Gly Met Asp Lys Thr Leu Gln Thr Leu Ala Gln Tyr Tyr
100 105 110
Pro Leu Ala Ile Val Ser Ala Arg Asp Glu Glu Ala Thr Leu Ala Phe
115 120 125
Leu Gln Gln Phe Asp Leu Thr Ser Leu Phe Gln Val Ile Val Ser Ala
130 135 140
His Thr Cys Lys Phe Thr Lys Pro Phe Pro Asp Pro Ile Leu Trp Ala
145 150 155 160
Ala Glu Lys Leu Asn Val Pro Pro Ser Ala Cys Leu Met Val Gly Asp
165 170 175
Thr Thr Val Asp Ile His Ala Gly Lys Lys Ala Gly Ala Gln Thr Val
180 185 190
Gly Val Leu Cys Gly Phe Gly Thr His Lys Glu Leu Val Cys Ala Gly
195 200 205
Ala Asp Leu Ile Leu Glu Thr Thr Ser Glu Leu Ala Glu Ile Leu Leu
210 215 220
Lys Glu Lys Asn Ser Met Pro Asn
225 230
<210> 12
<211> 252
<212> PRT
<213> Archaeoglobus fugidus
<400> 12
Met Asp Glu Arg Asp Ala Leu Arg Ile Ser Arg Glu Ile Ala Gly Glu
1 5 10 15
Val Arg Lys Ala Ile Ala Ser Met Pro Leu Arg Glu Arg Val Lys Asp
20 25 30
Val Gly Met Gly Lys Asp Gly Thr Pro Thr Lys Ala Ala Asp Arg Val
35 40 45
Ala Glu Asp Ala Ala Leu Glu Ile Leu Arg Lys Glu Arg Val Thr Val
50 55 60
Val Thr Glu Glu Ser Gly Val Leu Gly Glu Gly Asp Val Phe Val Ala
65 70 75 80
Leu Asp Pro Leu Asp Gly Thr Phe Asn Ala Thr Arg Gly Ile Pro Val
85 90 95
Tyr Ser Val Ser Leu Cys Phe Ser Tyr Ser Asp Lys Leu Lys Asp Ala
100 105 110
Phe Phe Gly Tyr Val Tyr Asn Leu Ala Thr Gly Asp Glu Tyr Tyr Ala
115 120 125
Asp Ser Ser Gly Ala Tyr Arg Asn Gly Glu Arg Ile Glu Val Ser Asp
130 135 140
Ala Glu Glu Leu Tyr Cys Asn Ala Ile Ile Tyr Tyr Pro Asp Arg Lys
145 150 155 160
Phe Pro Phe Lys Arg Met Arg Ile Phe Gly Ser Ala Ala Thr Glu Leu
165 170 175
Cys Phe Phe Ala Asp Gly Ser Phe Asp Cys Phe Leu Asp Ile Arg Pro
180 185 190
Gly Lys Met Leu Arg Ile Tyr Asp Ala Ala Ala Gly Val Phe Ile Ala
195 200 205
Glu Lys Ala Gly Gly Lys Val Thr Glu Leu Asp Gly Glu Ser Leu Gly
210 215 220
Asn Lys Lys Phe Asp Met Gln Glu Arg Leu Asn Ile Val Ala Ala Asn
225 230 235 240
Glu Lys Leu His Pro Lys Leu Leu Glu Leu Ile Lys
245 250
<210> 13
<211> 344
<212> PRT
<213> Archaeoglobus fugidus
<400> 13
Met Phe Val Met Phe Ile Val Ala Glu Val Asp Asp Glu Val Cys Val
1 5 10 15
Lys Arg Glu Val Glu Arg Ala Ala Gly Glu Val Gly Val His Val Ser
20 25 30
Leu Thr Pro Phe Gln Arg Arg Glu Lys Ala Glu Lys Asn Leu Tyr Val
35 40 45
Val Thr Ile Leu Gly Lys Asp Arg Val Gly Ile Val Arg Asp Ile Thr
50 55 60
Arg Ala Phe Leu Asp Phe Gly Ile Asn Ile Glu Arg Thr Ser Leu Thr
65 70 75 80
Ala Arg Glu Glu Leu Ile Ser Ile Glu Phe Leu Val Asp Leu Gly Gln
85 90 95
Arg Asp Ala Ala Glu Val Arg Lys Arg Leu Arg Arg Glu Ala Glu Arg
100 105 110
Leu Gly Leu Asp Ile Val Met Gln Pro Tyr Ser Thr Phe Asn Arg Glu
115 120 125
Lys Arg Leu Ile Val Phe Asp Met Asp Ser Thr Leu Val Glu Ala Glu
130 135 140
Ile Ile Asp Glu Leu Ala Lys Glu Ala Gly Val Gly Asp Glu Val Ser
145 150 155 160
Lys Leu Thr Glu Arg Ala Met Arg Gly Glu Ile Gly Phe Lys Glu Ala
165 170 175
Leu Glu Glu Arg Val Arg Leu Leu Lys Gly Leu Pro Val Glu Val Leu
180 185 190
Glu Arg Ile Tyr Ser Arg Ile Lys Leu Thr Glu Gly Ala Lys Glu Leu
195 200 205
Val Arg Ser Leu Lys Glu Ala Gly Tyr Lys Val Ala Val Val Ser Gly
210 215 220
Gly Phe Ser Tyr Phe Thr Asp Arg Leu Lys Glu Glu Leu Gly Leu Asp
225 230 235 240
Tyr Ala Phe Gly Asn Glu Leu Glu Ile Glu Asn Gly Arg Leu Thr Gly
245 250 255
Arg Ile Lys Gly Arg Ile Ile Asp Ala Ser Glu Lys Ala Arg Ile Val
260 265 270
Glu Glu Ile Ala Arg Lys Glu Gly Ile Ser Pro Glu Asn Val Val Ala
275 280 285
Val Gly Asp Gly Ala Asn Asp Arg Leu Met Ile Glu Arg Ala Gly Leu
290 295 300
Gly Ile Ala Phe Asn Ala Lys Glu Val Leu Lys Asp Val Ala Asp Gly
305 310 315 320
Ser Ile Ser Lys Glu Asn Leu Val Gly Leu Ala Ser Val Leu Lys Leu
325 330 335
Pro Ala Glu Phe Arg Lys Lys Val
340
<210> 14
<211> 277
<212> PRT
<213> Archaeoglobus fugidus
<400> 14
Met Leu Lys Thr Pro Ser Asp Ala Ala Val Ala Gly Cys Leu Ser Leu
1 5 10 15
Leu Leu Glu Val Ser Gly Asn Pro Lys Ala Gly Asn Val Asp Arg Glu
20 25 30
His Asp Phe Asp Asp Leu Lys Phe Glu His Phe Leu Ala Ser Ala Ala
35 40 45
Gly Ala Phe Pro Ala Phe Leu Glu Val Ala Glu Lys Arg Ile Ile Gly
50 55 60
Glu Gly Val Leu Arg Ala Val Lys Glu Ser Met Arg Trp His Arg Ala
65 70 75 80
Glu Asn Val His Phe Gly Ala Phe Leu Leu Leu Val Pro Leu Ile Ser
85 90 95
Ser Trp Asp Ala Gly Gly Met Val Asp Ile Ala Glu Ala Ala Arg Asn
100 105 110
Arg Leu Arg Arg Thr Asp Phe Arg Asp Ser Leu Ser Val Leu Glu Ala
115 120 125
Phe Arg Leu Ser Asn Ala Arg Val Val Glu Ala Gly Glu Leu Asn Leu
130 135 140
Lys Asp Arg Lys Thr Glu Glu Glu Ile Ala Gln Lys Lys Ile Asn Leu
145 150 155 160
Tyr Glu Trp Met Lys Met Ala Pro Glu Glu Asn Leu Ile Ala Arg Glu
165 170 175
Leu Val Asp Gly Phe Lys Ile Ser Ile Glu Gly Ala Lys Phe Leu Leu
180 185 190
Ser Phe Gly Asn Ser Gly Lys Ala Val Val Glu Leu Tyr Tyr His Leu
195 200 205
Leu Ser Lys Phe Pro Asp Pro Leu Val Ile Ala Lys Met Gly Arg Glu
210 215 220
Tyr Ala Glu Lys Ile Thr Glu Trp Ala Glu Lys Ala Arg Thr Glu Glu
225 230 235 240
Glu Arg Lys Glu Leu Asp Glu Lys Leu Leu Lys Asp Gly Ala Asn Pro
245 250 255
Gly Thr Ile Ala Asp Leu Thr Ala Ser Ser Ile Phe Leu Ala Leu Ala
260 265 270
Glu Gly Trp Arg Ile
275
<210> 15
<211> 219
<212> PRT
<213> Archaeoglobus profundus
<400> 15
Met Phe Lys Ala Leu Val Val Asp Ile Asp Gly Thr Leu Thr Asp Lys
1 5 10 15
Lys Arg Ala Ile Asn Cys Arg Ala Val Glu Ala Leu Arg Lys Leu Lys
20 25 30
Ile Pro Val Val Leu Ala Thr Gly Asn Ile Ser Cys Phe Ala Arg Ala
35 40 45
Val Ala Lys Ile Ile Gly Val Ser Asp Ile Val Ile Ala Glu Asn Gly
50 55 60
Gly Val Val Arg Phe Ser Tyr Asp Gly Glu Asp Ile Val Leu Gly Asp
65 70 75 80
Arg Ser Lys Cys Leu Arg Ala Leu Glu Thr Leu Arg Lys Arg Phe Lys
85 90 95
Val Glu Leu Leu Asp Asn Glu Tyr Arg Lys Ser Glu Val Cys Met Arg
100 105 110
Arg Asn Phe Pro Ile Glu Glu Ala Arg Lys Ile Leu Pro Lys Asp Val
115 120 125
Arg Ile Val Asp Thr Gly Phe Ala Tyr His Ile Ile Asp Ala Asn Val
130 135 140
Ser Lys Gly Lys Ala Leu Met Phe Ile Ala Asp Lys Leu Gly Leu Asp
145 150 155 160
Val Lys Asp Phe Ile Ala Ile Gly Asp Ser Glu Asn Asp Ile Glu Met
165 170 175
Leu Glu Val Ala Gly Phe Gly Val Ala Val Ala Asn Ala Asp Glu Lys
180 185 190
Leu Lys Glu Val Ala Asp Leu Val Thr Ser Lys Pro Asn Gly Asp Gly
195 200 205
Val Val Glu Ala Leu Glu Phe Leu Gly Leu Ile
210 215
<210> 16
<211> 224
<212> PRT
<213> 嗜热硫酸盐还原菌(Archaeoglobus veneficus)
<400> 16
Met Leu Arg Pro Lys Gly Leu Ala Ile Asp Ile Asp Gly Thr Ile Thr
1 5 10 15
Tyr Arg Asn Arg Ser Leu Asn Cys Lys Ala Val Glu Ala Leu Arg Lys
20 25 30
Val Lys Ile Pro Val Val Leu Ala Thr Gly Asn Ile Ser Cys Phe Ala
35 40 45
Arg Thr Ala Ala Lys Leu Ile Gly Val Ser Asp Ile Val Ile Cys Glu
50 55 60
Asn Gly Gly Ile Val Arg Phe Ser Tyr Asp Gly Asp Asp Ile Val Leu
65 70 75 80
Gly Asp Ile Ser Lys Cys Leu Lys Ala Ala Glu Ile Leu Lys Glu Tyr
85 90 95
Phe Glu Ile Glu Phe Leu Asp Ala Glu Tyr Arg Lys Ser Glu Val Cys
100 105 110
Leu Arg Arg Asn Phe Pro Ile Glu Glu Ala Arg Lys Ile Leu His Asp
115 120 125
Ala Lys Leu Asp Val Lys Ile Val Asp Ser Gly Phe Ala Tyr His Ile
130 135 140
Met Asp Ala Lys Val Ser Lys Gly Arg Ala Leu Glu Tyr Ile Ala Asp
145 150 155 160
Glu Leu Gly Ile Ser Pro Lys Glu Phe Ala Ala Ile Gly Asp Ser Glu
165 170 175
Asn Asp Ile Asp Leu Ile Lys Ala Ala Gly Leu Gly Ile Ala Val Gly
180 185 190
Asp Ala Asp Leu Lys Leu Lys Met Glu Ala Asp Val Val Val Ser Lys
195 200 205
Lys Asn Gly Asp Gly Val Val Glu Ala Leu Glu Leu Leu Gly Leu Ile
210 215 220
<210> 17
<211> 194
<212> PRT
<213> 地衣芽孢杆菌(Bacillus licheniformis)
<400> 17
Met Leu His Leu Gly Ile Asp Ile Asp Gly Thr Ile Thr Ala Gln Asp
1 5 10 15
Thr Phe Val Pro Tyr Leu Asn Glu Ser Phe Ser Leu Ser Ile Thr Leu
20 25 30
Glu Asp Ile Lys Glu Tyr Asp Leu Thr Lys Leu Leu Asn Ile Ser Gln
35 40 45
Glu Asp Phe Trp Lys Trp Met Asn Glu Asn Glu Pro Arg Ile Tyr Gln
50 55 60
Glu Ala Leu Leu Ala Gln Tyr Ala Lys Gln Thr Leu Asp Glu Leu Lys
65 70 75 80
Lys Gln His Lys Leu Ile Tyr Ile Thr Ala Arg Arg His His Leu Glu
85 90 95
Asp Ile Thr Tyr Asp Trp Phe Glu Lys Arg Asp Ile His Tyr Asp His
100 105 110
Ile Glu Leu Val Gly Gly His Asp Lys Leu Glu Ala Val Phe Arg His
115 120 125
Glu Ile Asp Val Phe Phe Glu Asp His His Gly Asn Ala Thr Met Ile
130 135 140
Ala Lys Glu Ala Gly Ile Pro Val Leu Leu Phe Asn Thr Pro Tyr Asn
145 150 155 160
Gln Leu Pro Thr Asp Ser Asn Ile Val Arg Val Asp Ser Trp Leu Glu
165 170 175
Ala Ala Glu Trp Leu Lys Lys Asn Glu Lys Thr Leu Lys Ala Ala Lys
180 185 190
Ser Met
<210> 18
<211> 226
<212> PRT
<213> 地衣芽孢杆菌(Bacillus licheniformis)
<400> 18
Met Lys Ala Val Ile Phe Asp Leu Asp Gly Val Ile Thr Asp Thr Ala
1 5 10 15
Glu Tyr His Tyr Leu Ala Trp Lys His Thr Ala Glu Gln Ile Gly Ile
20 25 30
Glu Ile Asp Arg Ser Phe Asn Glu Arg Leu Lys Gly Ile Asn Arg Glu
35 40 45
Gln Ser Leu Asp Lys Ile Leu Ile His Gly Gly Ala Ala Gly Lys Phe
50 55 60
Gln Glu Ala Glu Lys Gln Glu Ile Met Arg Arg Lys Asn Gln Tyr Tyr
65 70 75 80
Gln Gln Leu Ile Gln Asn Leu Thr Pro His Asp Leu Leu Pro Gly Ile
85 90 95
Ser Val Leu Phe Ala Glu Leu Lys Arg Glu His Ile Ser Ile Ala Leu
100 105 110
Ala Ser Ser Ser Arg Asn Ala Pro Ala Ile Leu Gln Arg Leu Gly Val
115 120 125
Met Asp Glu Phe Gln Gly Val Val Asp Pro Ala Ala Leu Ala His Gly
130 135 140
Lys Pro Asp Pro Glu Ile Phe Leu Thr Ala Ala Ala Leu Leu Gly Val
145 150 155 160
Pro Pro Ser Glu Cys Ala Ala Ile Glu Asp Ala Glu Ala Gly Ile Ala
165 170 175
Ala Ile Lys Ser Ala Gly Met Phe Ala Val Gly Val Gly Asp Glu Thr
180 185 190
Ser Leu Arg Gly Ala Asp Leu Ile Val His Asn Thr Asn Glu Leu Thr
195 200 205
Phe Glu Leu Leu Asn Glu Gly Trp Gln Arg Tyr Cys Cys Ile Arg Glu
210 215 220
Gly Lys
225
<210> 19
<211> 216
<212> PRT
<213> 地衣芽孢杆菌(Bacillus licheniformis)
<400> 19
Met Lys Lys Trp Ala Phe Val Ser Asp Phe Asp Gly Thr Ile Ser Lys
1 5 10 15
Gln Asp Phe Tyr Trp Met Val Ile Asp Lys Tyr Phe Pro Glu Gly Arg
20 25 30
Glu Leu Phe Lys Lys Trp Lys Ser Gly Glu Leu Lys Asp Ile Glu Phe
35 40 45
Leu Gly Thr Val Phe Ala Ser Ile Asn Gln Ser Glu Gln Lys Ile Ile
50 55 60
Asp Asp Ile His Ser Ile Pro Ile Asp Glu Tyr Val Pro Asp Phe Ile
65 70 75 80
Gln His Val Gln Lys Ser Gly Gly Asp Phe Tyr Ile Leu Ser Ala Gly
85 90 95
Thr Asp Tyr Tyr Ile His Tyr Ile Leu Lys Lys Tyr Gly Ile Thr Asp
100 105 110
Val Glu Val Tyr Ser Asn Lys Gly Phe Phe Lys Glu Asp Asn Val His
115 120 125
Met Asp Ile Asp Glu Asn His Trp His Tyr Ser Glu Arg Tyr Gly Ile
130 135 140
Asp Lys Ser Lys Val Ile Gln Lys Leu Lys Glu Glu Tyr Glu Thr Val
145 150 155 160
Tyr Phe Ala Gly Asp Ser Glu Pro Asp Ser His Pro Ala Lys Phe Ala
165 170 175
Asp Val Thr Phe Ala Lys Asp Ala Leu Gln Asp Leu Leu Arg Gln Gln
180 185 190
Gly Val Pro Phe Val Ala Val Glu Thr Phe Glu Asp Ile Glu Gln Tyr
195 200 205
Leu Lys Glu Lys Gly Arg Ile Val
210 215
<210> 20
<211> 223
<212> PRT
<213> 热解纤维素菌(Caldicellulosiruptor bescii)
<400> 20
Met Gly Lys Ile Lys Ala Ala Ile Phe Asp Met Asp Gly Val Leu Thr
1 5 10 15
Asp Thr Val Lys Leu His Phe Lys Ala Trp Lys Lys Met Phe Glu Ser
20 25 30
His Gly Tyr Lys Phe Glu Tyr Glu Asp Tyr Lys Trp Lys Val Asp Gly
35 40 45
Lys Pro Arg Ile Asp Gly Ile Arg Ser Ile Ala Tyr Asp Met Pro Glu
50 55 60
Asp Lys Leu Ile Glu Met Ala Glu Glu Lys Gln Lys Ile Phe Leu Glu
65 70 75 80
Phe Val Glu Gln Glu Asn Leu Glu Ala Phe Glu Asp Ser Ile Trp Leu
85 90 95
Leu Asn His Leu Lys Gln Asn Asp Ile Lys Leu Ala Val Ala Ser Ser
100 105 110
Ser Lys Asn Thr Thr Lys Ile Leu Thr Lys Ile Gly Ile Tyr Asn Met
115 120 125
Phe Asp Thr Ile Val Thr Gly Tyr Asp Phe Lys Lys Gly Lys Pro Asp
130 135 140
Pro Glu Ile Phe Leu Thr Ala Ala Gln Arg Leu Asn Val Asn Pro Lys
145 150 155 160
Glu Cys Val Val Phe Glu Asp Ala Ile Asp Gly Val Lys Ala Gly Ile
165 170 175
Arg Ala Gly Met Leu Thr Ile Gly Val Cys Arg Asp Gly Gln Phe Asp
180 185 190
Arg Leu Lys Glu Ala His Tyr Val Val Asp Arg Leu Asp Lys Ile Ser
195 200 205
Leu Glu Leu Leu Glu Asn Leu His Glu Lys Leu Phe Lys Lys Val
210 215 220
<210> 21
<211> 275
<212> PRT
<213> 热解纤维素菌(Caldicellulosiruptor bescii)
<400> 21
Met Lys Asn Ser Ser Ile Leu Lys Asn Ile Asp Leu Phe Leu Leu Asp
1 5 10 15
Leu Asp Gly Thr Val Tyr Leu Gly Glu Lys Val Phe Glu Gly Ala Arg
20 25 30
Glu Phe Ile Lys Leu Leu Asn Lys Asn Gln Lys Glu Phe Leu Phe Leu
35 40 45
Thr Asn Asn Ser Ser Lys Ser Ser Glu Glu Tyr Tyr Ser Lys Leu Leu
50 55 60
Asn Met Gly Phe Glu Ile Thr Lys Glu Asn Val Phe Thr Ser Gly Gln
65 70 75 80
Ala Met Gly Ile Tyr Ile Lys Thr Ile His Lys Lys Glu Lys Pro Pro
85 90 95
Arg Val Tyr Val Val Gly Thr Thr Ser Leu Lys Arg Glu Leu Lys Ser
100 105 110
Met Gly Ile Val Val Val Asp Ser Pro Asn Tyr Asn Ile Asp Tyr Leu
115 120 125
Val Ile Gly Phe Asp Thr Thr Leu Thr Tyr Lys Lys Leu Leu Asp Ala
130 135 140
Cys Glu Leu Ile Arg Arg Gly Val Pro Phe Leu Ala Thr Asn Pro Asp
145 150 155 160
Leu Val Cys Pro Leu Asp Gly Gly Arg Tyr Ile Pro Asp Cys Gly Ser
165 170 175
Ile Cys Ile Met Leu Glu Asn Ala Thr Lys Lys Lys Pro Val Phe Val
180 185 190
Gly Lys Pro Ser Ser Ile Met Val Asp Ile Ile Ser Asn Leu Lys Lys
195 200 205
Val Glu Lys Ser Arg Ile Ala Met Ile Gly Asp Arg Leu Tyr Thr Asp
210 215 220
Met Lys Met Ala Lys Asp Ser Gly Met Val Ala Ala Leu Val Leu Ser
225 230 235 240
Gly Glu Thr Lys Met Lys Asp Val Glu Ala Ser Thr Leu Lys Pro Asp
245 250 255
Leu Ile Tyr Gly Ser Ile Lys Asp Met Tyr Glu Glu Leu Lys Leu Val
260 265 270
Phe Gly Gly
275
<210> 22
<211> 266
<212> PRT
<213> 热解纤维素菌(Caldicellulosiruptor bescii)
<400> 22
Met Ile Lys Leu Val Ala Thr Asp Leu Asp Asp Thr Leu Leu Ser Lys
1 5 10 15
Asp Leu Thr Ile Thr Glu Lys Asn Leu Asn Ala Ile Glu Phe Leu Lys
20 25 30
Lys Asn Asn Ile Ile Phe Ile Leu Ala Ser Gly Arg Pro Tyr Pro Ser
35 40 45
Ile Lys Asn Val Ala Tyr Asp Leu Gln Asn Phe Tyr Pro Met Ile Thr
50 55 60
Tyr Gln Gly Ala Leu Val Tyr Asp Pro Lys Asn Asp Lys Lys Leu Tyr
65 70 75 80
Gly Cys Glu Ile Lys Pro Glu Asp Ala Lys Glu Leu Val Arg Leu Ala
85 90 95
Lys Asp Glu Gly Ile His Val His Ile Tyr Ile Asp Asn Val Trp Tyr
100 105 110
Val Glu Ala Met Asn Glu Lys Thr Glu Tyr Tyr Arg Asn Leu Thr Lys
115 120 125
Leu Glu Pro His Ile Val Lys Asn Leu Leu Glu Phe Ile Asp Arg Pro
130 135 140
Val Thr Lys Val Leu Phe Phe Asp Glu His Glu Arg Leu Lys Asp Leu
145 150 155 160
Lys Glu Ser Leu Pro Asp Asp Phe Ser Lys Lys Phe Asn Ile Met Phe
165 170 175
Ser Lys Pro Phe Phe Leu Glu Phe Thr Asp Ile Asn Val Ser Lys Gly
180 185 190
Asn Ala Leu Lys Phe Leu Thr Glu Tyr Tyr Gly Leu Lys Arg Glu Glu
195 200 205
Val Met Ala Ile Gly Asp Gly Asp Asn Asp Ile Ser Met Ile Glu Tyr
210 215 220
Ala Gly Ile Gly Val Ala Val Glu Asn Ala Val Glu Lys Leu Lys Glu
225 230 235 240
Ala Ala Asp Phe Val Val Ala Lys Ser Asp Asp Ser Gly Phe Ala Gln
245 250 255
Ala Ile Glu Lys Val Phe Asn Val His Phe
260 265
<210> 23
<211> 279
<212> PRT
<213> 热解纤维素菌(Caldicellulosiruptor bescii)
<400> 23
Met Tyr Lys Leu Ile Ala Ile Asp Leu Asp Met Thr Leu Leu Asp Lys
1 5 10 15
Asn Lys Asn Ile Ser Ser Arg Asn Lys Arg Ala Ile Glu Leu Val Lys
20 25 30
Gln Lys Gly Val Gln Ile Val Leu Cys Ser Gly Arg Ile Leu Lys Gly
35 40 45
Val Met Tyr Phe Ala Lys Val Leu Gly Leu Tyr Asp Gln Val Ile Val
50 55 60
Ala Cys Asn Gly Ala Ile Val Arg Asp Leu Lys Lys Asn Asn Asp Ile
65 70 75 80
Tyr Tyr Ile Gly Leu Glu Asn Ser Lys Ser Leu Glu Ile Ala Arg Ile
85 90 95
Cys Lys Glu Asn Asp Ile Tyr Tyr His Tyr Tyr Phe Gln Asp Thr Met
100 105 110
Ile Ala Arg Arg Leu Asp Tyr Ser Ser Lys Phe Tyr Tyr Glu Lys Asn
115 120 125
Lys Glu Leu Pro Glu Glu Glu Arg Ile Asn Ile Ile Ile Asp Asp Ser
130 135 140
Glu Asn Thr Ile Asn Ala Cys Gly Asp Leu Ile Thr Lys Phe Val Ile
145 150 155 160
Ile Asp Lys Asp Leu Glu Lys Val Asn Tyr Val Arg Lys Ile Ile Glu
165 170 175
Arg Glu Ile Pro Gly Val Glu Thr Thr Lys Ser Asp Ile Asn Ile Leu
180 185 190
Glu Val Met Lys Glu Gly Val Asn Lys Lys Arg Ala Leu Glu Phe Val
195 200 205
Ile Ser Tyr Leu Gly Ile Ala Pro Glu Glu Val Met Ala Ile Gly Asp
210 215 220
Asn Glu Asn Asp Leu Glu Met Ile Glu Phe Ala Gly Leu Gly Val Ala
225 230 235 240
Met Gly Asn Ala Ile Glu Glu Leu Lys Lys Ile Ala Asp Tyr Val Thr
245 250 255
Ser Ser Tyr Glu Asn Asp Gly Val Ala Arg Ala Ile Glu Lys Phe Val
260 265 270
Leu Gly Glu Ala Val Asn Val
275
<210> 24
<211> 284
<212> PRT
<213> 热解纤维素菌(Caldicellulosiruptor bescii)
<400> 24
Met Ile Lys Leu Ile Ala Leu Asp Ile Asp Gly Thr Leu Leu Asp Asp
1 5 10 15
Arg Gly Tyr Ile Pro Gln Ile Asn Arg Glu Phe Leu Lys Ile Ala Val
20 25 30
Glu Lys Tyr Lys Ile Val Ile Val Leu Cys Thr Gly Arg Gly Ala Ser
35 40 45
Ala Phe Lys Ile Ala Lys Asp Leu Gln Leu Pro Cys Ser Leu Ile Ser
50 55 60
Ala Asn Gly Val Tyr Val Phe Glu Asn Pro Asn Phe Pro Pro Ile Ile
65 70 75 80
Lys Asn Tyr Leu Thr Glu Arg Gln Lys Lys Val Leu Ile Glu Phe Leu
85 90 95
Asp Asn Asn His Phe Glu Ile Asp Tyr Tyr Ile Val Leu Gly Tyr Glu
100 105 110
Gln Asp Phe His Met Ile Tyr Lys Glu Arg Thr Asn Tyr Asp Ser Tyr
115 120 125
Phe Leu Ser Phe Val Asn Gly Arg Lys Gln Phe Lys Ser Thr Tyr Pro
130 135 140
Ala Glu Lys Leu Leu Lys Phe Leu Glu Tyr Pro Ile Ser His Thr Gly
145 150 155 160
Ile Val Gly Lys Tyr Glu Lys Leu Lys Glu Ile Lys Glu Ile Leu Lys
165 170 175
Thr Leu Glu Leu Asp Cys Asn Ile Ile Leu Tyr Tyr Ala Ser Asp Asn
180 185 190
Lys Glu Tyr Gly Phe Leu Glu Val Leu Ser Asn Asn Ala Ser Lys Glu
195 200 205
Lys Ala Leu Leu Gln Phe Met Asn Phe Lys Asn Ile Ser Ser Glu Glu
210 215 220
Leu Ile Ser Ile Gly Asp Asn Phe Asn Asp Val Gly Met Phe Lys Ile
225 230 235 240
Ser Gly Ile Ser Val Ala Val Ala Asn Ala Pro Glu Glu Val Lys Lys
245 250 255
Ala Ala Lys Phe Val Thr Ser Arg Thr Asn Asn Glu Gly Ala Val Ala
260 265 270
Glu Ala Ile Glu Met Phe Ile Ile Lys Arg Asp Gly
275 280
<210> 25
<211> 286
<212> PRT
<213> Caldilinea aerophila
<400> 25
Met Arg Pro Phe Asp Leu Val Val Leu Asp Leu Asp Gly Thr Ile Leu
1 5 10 15
Asn Ala Tyr Gln Arg Thr Arg Ile Ser Lys Ala Val His Asp Ala Ile
20 25 30
Glu Ala Val Gln Ala Ala Gly Val Pro Val Thr Ile Gly Thr Gly Arg
35 40 45
Thr Leu Asp Tyr Ile Arg Tyr His Leu Pro Gly Asp Leu His Leu Thr
50 55 60
Tyr Pro Val Ile Ala Thr Gln Gly Ala Val Ile Gly Asp Pro Val Ser
65 70 75 80
Gly Arg Val Leu Val Glu Ile Pro Leu Pro Leu Lys Glu Ala Arg Ala
85 90 95
Ile Ala Ala Phe Val Asp Asp His Arg Tyr Thr Thr Ala Phe Tyr Phe
100 105 110
Asn Asn Ala Asp Gly His Thr Val Ile Tyr Gln Asn Ala Ala Gly Ala
115 120 125
Thr Ala Glu Glu Glu Ala Leu Leu His His Leu Leu Gly Val Pro Asn
130 135 140
Ala Leu Val Glu Gln Phe Ser Pro Leu Leu Ala Asn Glu Asp Ala His
145 150 155 160
Pro Pro Ile Lys Val Ile Ser Phe Asn His Gly Leu Pro Asp Ala Val
165 170 175
Asp Leu Leu His Glu Tyr Gln Arg Arg Phe Ser Pro Pro Leu Thr Val
180 185 190
Thr Arg Thr His Glu Trp Leu Val Glu Ala Thr Ala Pro Gly Val Asp
195 200 205
Lys Gly Ser Gly Leu Leu Arg Leu Cys Lys Leu Leu Gly Val Asp Pro
210 215 220
Gln Arg Val Leu Ala Ile Gly Asp Ser Asp Asn Asp Ile Pro Met Leu
225 230 235 240
Glu Val Ala Gly Phe Ala Ile Ala Met Gly Asn Ala Asn Glu Arg Val
245 250 255
Lys Ala Val Ala Asp Trp Val Ala Pro Ser Ile Asp Glu Glu Gly Ala
260 265 270
Ala Val Ala Leu Arg Arg Trp Val Leu Glu Pro Met Ser Ala
275 280 285
<210> 26
<211> 256
<212> PRT
<213> Caldilinea aerophila
<400> 26
Met Lys Asp Lys Thr Lys Val Leu Ala Leu Asp Leu Asp Asp Thr Leu
1 5 10 15
Leu Arg Ser Asp Lys Ser Ile Gly Ala Ala Thr Leu Ala Ala Leu Asp
20 25 30
Arg Trp Leu Ala Ala Gly His Glu Ile Val Val Ala Thr Gly Arg Pro
35 40 45
Pro Arg Asn Val Ala Pro Val Leu Pro Pro Ser Leu Leu His Val Pro
50 55 60
Arg Ile Val Tyr Asn Gly Ala Gln Ala Ile Leu Gly Asp Glu Ile Ile
65 70 75 80
Phe Asp Arg Pro Ile Pro Ala Gln Asp Val Arg His Ile Leu Glu Trp
85 90 95
Ser Lys Arg Ser Gly Glu Ala Trp Tyr Ile Gly Leu Glu Ile Asn Asp
100 105 110
Met Leu Tyr Val Asn Arg His Phe Glu Lys Pro Gly Thr Phe Gln Val
115 120 125
Val Asp Leu Met Gln Leu Cys Asp Gln Pro Val Tyr Lys Ile Ile Phe
130 135 140
Phe Phe Pro Asp Gly Arg Arg Asn Ile Glu Pro Leu Leu Ala Ala Met
145 150 155 160
Pro Pro Thr Thr Arg Ala Leu Val Thr Pro Lys Phe Ser Leu Val Gln
165 170 175
Leu Cys Ala Arg Asp Ala Asn Lys Phe Thr Ala Leu Lys Gln Leu Leu
180 185 190
Ala Arg Arg Pro Leu Ala Pro Ala Ala Val Ile Ala Val Gly Asp Asp
195 200 205
Ile Asn Asp Ile Glu Met Val Arg Cys Ser Asp Ile Gly Val Ala Val
210 215 220
Ala Asn Ala Leu Pro Glu Val Lys Ala Val Ala Asp Trp Ile Ala Pro
225 230 235 240
Gly Ala Asp Asp Asp Gly Val Ala Tyr Val Ile Asp Arg Leu Leu Asn
245 250 255
<210> 27
<211> 223
<212> PRT
<213> Caldilinea aerophila
<400> 27
Met Thr Ile Arg Ala Ile Leu Trp Asp Leu Asp Gly Thr Leu Ala Asp
1 5 10 15
Thr Thr Ala Leu His Tyr Gln Ala Trp Arg Lys Thr Met Gln Arg Tyr
20 25 30
Gly Val Asp Leu Thr Tyr Glu Ser Phe Ile Lys Asp Tyr Gly Arg Asn
35 40 45
Asn Ala Glu Ile Leu Ala Glu His Phe Gly Ile His Ala Val Ala Thr
50 55 60
Ile Gln Gln Val Ala Asp Glu Lys Glu Ser Ala Phe Arg Ser Leu Ile
65 70 75 80
Thr Pro Gly Val Leu Gln Pro Leu Pro Gly Ala Leu Ala Trp Leu His
85 90 95
Cys Phe Arg Ser Leu Gly Met Ile Gln Val Ile Gly Ser Ser Gly Pro
100 105 110
Met Ala Asn Ile Ala Ala Val Val His Ala Leu Gly Val Gly Asp Phe
115 120 125
Phe Phe Gly Leu Val Ser Gly Val His Leu Pro Glu Gly Lys Pro Glu
130 135 140
Pro Thr Ile Phe Leu Arg Cys Ala Ala Leu Ala Gly Ala Ala Pro Asp
145 150 155 160
Glu Cys Leu Val Ile Glu Asp Ser Leu His Gly Ile Glu Ala Ala Met
165 170 175
Arg Ala Gly Met Thr Ser Val Ala Val Gly Arg Ile Ala Ala Gln Pro
180 185 190
Ala Leu Gln Asp Leu Ile Gln Lys Asn Pro Gly Leu Arg Cys Ile Val
195 200 205
Thr Pro Ser Leu Ala Ser Ile Asp Asp Pro Lys Ser Ile Leu Thr
210 215 220
<210> 28
<211> 275
<212> PRT
<213> Caldithrix abyssi
<400> 28
Met Ser Glu Val Met Lys Lys Met Leu Val Leu Asp Phe Asp Gly Thr
1 5 10 15
Leu Phe Gln Pro Gly Lys Phe Ile Ser Asp Gln Asp Leu Lys Leu Leu
20 25 30
Lys Ala Leu Ala Asn Met Asn Val Val Arg Val Ile Ala Thr Gly Arg
35 40 45
Ser Leu Phe Ser Leu Lys Lys Val Ile Asp Met Ser Phe Pro Ile Asp
50 55 60
Tyr Leu Ile Leu Ser Thr Gly Val Gly Ile Leu Asp Trp Arg Ser Gln
65 70 75 80
Lys Leu Ile Arg Ser Tyr Asp Met Glu Gly Gly Leu Val Asp Glu Ile
85 90 95
Ile Ala Ile Leu Lys Arg Arg Arg His Ser Phe Phe Val His Gln Pro
100 105 110
Ala Pro Gln Asn His Lys Phe Phe Tyr Tyr Asp Gly Ile Ala Pro Arg
115 120 125
Gln Asp Phe Thr Arg Arg Phe Glu Leu Tyr Arg Asp Phe Ala Arg Pro
130 135 140
Leu Lys Gln Arg Lys Arg Gly Met Ala Ala Gly Gln Ile Leu Val Ile
145 150 155 160
Ser Glu Glu Leu Gln Gln Ile Lys Glu Glu Leu Gln Pro Leu Met His
165 170 175
Arg Ile Asn Ile Ile Arg Thr Thr Ser Pro Leu Asp Gly Arg Ser Met
180 185 190
Trp Val Glu Ile Phe Asp Lys Arg Val Asn Lys Ala Leu Ala Ala Gln
195 200 205
Phe Leu Ala Glu Arg Leu Asn Ile Ser Val Arg Asn Ile Val Ala Leu
210 215 220
Gly Asn Asp Tyr Asn Asp Leu Asp Leu Leu Asn Trp Ala Gly Ser Ala
225 230 235 240
Phe Val Val His Thr Ala Pro Glu Glu Leu Lys Glu Lys Phe Gln Ile
245 250 255
Ile Lys Asn Ala Asp Asp Gly Leu Leu Ile His Ile Met Pro Leu Leu
260 265 270
Gln Arg Gly
275
<210> 29
<211> 171
<212> PRT
<213> Caldithrix abyssi
<400> 29
Met Arg Lys Lys Leu Ser Gln Ile Gln Leu Ile Leu Met Asp Val Asp
1 5 10 15
Gly Val Leu Thr Ala Gly Glu Ile Ile Tyr Ser Ala Ala Gly Asp Glu
20 25 30
Leu Lys Met Phe Asn Val Gln Asp Gly Met Gly Ile Thr Leu Ala Arg
35 40 45
Met Ala Gly Leu Lys Thr Gly Ile Leu Thr Gly Arg Lys Ser Glu Leu
50 55 60
Val Arg Arg Arg Ala Glu Glu Leu Lys Ile Asp Ile Val Ser Gln Gly
65 70 75 80
Ser Phe Glu Lys Leu Pro Glu Tyr Glu Lys Ile Lys Gln Gln Met Gln
85 90 95
Leu Ser Asp Glu Gln Ile Cys Tyr Ile Gly Asp Asp Val Leu Asp Ile
100 105 110
Pro Val Leu Lys Arg Val Gly Phe Ser Val Ala Val Ala Asn Ala Arg
115 120 125
Asp Glu Val Lys Ala Ile Ala Asp Tyr Val Thr Val Ala Glu Gly Gly
130 135 140
Lys Gly Ala Val Arg Glu Val Ile Asp Lys Ile Leu Lys Trp Gln Asn
145 150 155 160
Lys Leu His Ala Leu Ile Asp Gln Leu Ser Arg
165 170
<210> 30
<211> 354
<212> PRT
<213> Caldithrix abyssi
<400> 30
Met Lys Arg Gly His Arg Tyr Ile Val Phe Thr Met Ala Ile Phe Ser
1 5 10 15
Leu Ile Leu Ile Ala Cys Thr Lys Lys Glu Val Lys Pro Asp Leu Ala
20 25 30
Phe Ala Gly Glu Lys His Leu Lys Asn Ile Arg Met Leu Thr Asn Gly
35 40 45
Gly Glu Asn Ala Glu Ala Tyr Phe Ser Phe Asp Glu Ser Gln Leu Ile
50 55 60
Tyr Gln Ser Lys His Asp Ser Ile Lys Cys Asp Gln Ile Phe Ile Met
65 70 75 80
Asn Leu Asp Gly Ser Asp Lys Arg Met Val Ser Thr Gly Lys Gly Arg
85 90 95
Thr Thr Cys Ser Tyr Phe Leu Pro Gly Asp Gln Gln Ile Ile Tyr Ala
100 105 110
Ser Thr His Gly Val Asp Glu Asn Cys Pro Pro Pro Pro Asp Phe Ser
115 120 125
Arg Gly Tyr Val Trp Lys Val Tyr Ser Ser Tyr Asp Leu Tyr Ile Ala
130 135 140
Asn Ala Asp Gly Ser Asp Pro Gln Pro Phe Leu Pro Ser Pro Gly Tyr
145 150 155 160
Asp Ala Glu Ala Thr Val Ser Pro Arg Gly Asp Lys Ile Val Phe Thr
165 170 175
Ser Gln Arg Asn Gly Asp Leu Asp Ile Tyr Thr Val Asn Ile Asp Gly
180 185 190
Ser Gly Leu Lys Gln Leu Thr His Glu Ile Gly Tyr Asp Gly Gly Ala
195 200 205
Phe Phe Ser Trp Asp Gly Ser Lys Ile Val Tyr Arg Ala Tyr His Pro
210 215 220
Arg Thr Glu Glu Glu Leu Lys Arg Tyr Arg Lys Leu Leu Ala Glu Glu
225 230 235 240
Leu Ile Glu Pro Asn Asn Phe Gln Leu Phe Val Met Asp Ala Asp Gly
245 250 255
Ser Asn Lys Arg Gln Ile Thr His Asn Asp Phe Ala Asn Phe Ala Pro
260 265 270
Phe Phe His Pro Asp Asn Lys Arg Ile Ile Phe Cys Ser Asn Met Gly
275 280 285
Thr Thr Glu Ser Thr Gln Arg Asp Phe Asn Leu Trp Met Ile Asn Glu
290 295 300
Asp Gly Thr Gly Leu Lys Gln Ile Thr Phe Phe Lys Gly Phe Asp Gly
305 310 315 320
Phe Pro Met Phe Thr His Asp Gly Lys Lys Leu Val Phe Ala Ser Asn
325 330 335
Arg Phe Asn Lys Lys Lys Gly Glu Thr Asn Val Phe Ile Ala Asp Trp
340 345 350
Val Glu
<210> 31
<211> 222
<212> PRT
<213> Caldithrix abyssi
<400> 31
Met Arg Asn Lys Ala Val Leu Phe Asp Phe Asp Gly Val Val Val Lys
1 5 10 15
Ser Met Glu Gln His Phe Asn Ala Trp Arg Gln Ala Phe Leu Glu Lys
20 25 30
Gly Val Glu Ile Lys Glu Asp Glu Phe Phe Val Leu Glu Gly Gln Gly
35 40 45
Ile Asn Thr Ile Ala His His Leu Gly Lys Ile Tyr Gly Leu Asn Arg
50 55 60
Gln Gln Val Glu Glu Val Met Glu Arg Lys Val Asn Tyr Tyr Asn Gln
65 70 75 80
Phe Met Thr Leu Glu Phe Tyr Asp His Phe His Glu Leu Val Glu His
85 90 95
Leu His Arg Arg Gln Val Pro Met Gly Val Val Thr Gly Gly Asn Arg
100 105 110
Ser Arg Val Glu Lys Ile Ile Asn Glu His Phe Asn His Tyr Phe Arg
115 120 125
Ala Leu Val Thr Val Asp Asp Val Glu Arg Gly Lys Pro Phe Pro Asp
130 135 140
Pro Phe Leu Lys Ala Ala Gln Met Leu Asn Met Ala Pro Gln Asn Cys
145 150 155 160
Ile Val Val Glu Asn Ala Pro Met Gly Ile Lys Gly Ala Lys Arg Ala
165 170 175
Gly Met Thr Val Val Ala Ile Thr Thr Thr Leu Lys Pro Asp Tyr Leu
180 185 190
Lys Gln Ala Asp Tyr Ile Ala His Asn Phe Leu Glu Val Glu Glu Ile
195 200 205
Leu Asn Thr Leu Leu Gly Ile Glu Arg Thr Glu Thr Ile Lys
210 215 220
<210> 32
<211> 268
<212> PRT
<213> Carboxydocella sp. ULO1
<400> 32
Met Val Leu Val Lys Leu Ile Ala Ile Asp Leu Asp Asp Thr Leu Leu
1 5 10 15
Arg Thr Asp Cys Thr Ile Ser Pro Arg Ala Gln Ala Ala Ile Lys Ala
20 25 30
Ala Ser Ala Arg Gly Val Ala Val Thr Leu Ala Thr Gly Arg Met Tyr
35 40 45
Arg Ser Ala Arg Pro Tyr Ala Leu Glu Leu Gly Leu Asp Leu Pro Leu
50 55 60
Ile Thr Tyr Gln Gly Ala Leu Ile Lys Ser Gly Leu Ser Gly Glu Glu
65 70 75 80
Leu Trp His Cys Pro Leu Pro Pro Glu Met Ala Ala Glu Ile Ile Thr
85 90 95
Leu Ala Arg Glu Glu Gly Ile His Ala Asn Ile Tyr Leu Asp Asp His
100 105 110
Leu Tyr Val Glu Lys Ala Thr Ala Glu Ala Glu Ala Tyr Ser Gln Leu
115 120 125
Ala Arg Val Pro Tyr Thr Val Glu Glu Asp Leu Arg Gly Arg Ile Leu
130 135 140
Ala Ser Gly Gln Gly Pro Ser Lys Ile Leu Leu Ile Ala Glu Pro Glu
145 150 155 160
Arg Leu Asp Gln Leu Ala Ala Ala Leu Arg Pro Arg Trp Gln Gly Lys
165 170 175
Val Gln Leu Ala Lys Ser Lys Asp His Tyr Leu Glu Phe Thr His Pro
180 185 190
Glu Ala Ser Lys Gly Gln Ala Leu Ile Ala Leu Cys Arg Tyr Leu Asp
195 200 205
Leu Glu Leu Lys Asp Ala Met Ala Ile Gly Asp Ser Phe Asn Asp Leu
210 215 220
Asp Met Ile Glu Leu Ala Gly Ile Gly Val Ala Met Gly Asn Ala Lys
225 230 235 240
Pro Glu Val Lys Ala Ile Ala Asp Tyr Val Thr Leu Ala Asn Asp Asp
245 250 255
Asp Gly Val Ala Glu Ala Ile His Arg Phe Val Leu
260 265
<210> 33
<211> 266
<212> PRT
<213> Carboxydothermus ferrireducens
<400> 33
Met Arg Tyr Arg Leu Leu Ala Val Asp Leu Asp Asp Thr Phe Leu Asn
1 5 10 15
Lys Glu Leu Gln Val Ser Pro Arg Val Gln Gln Ala Val Ile Glu Ala
20 25 30
Leu Lys Lys Gly Ile Ile Val Thr Leu Ala Thr Gly Arg Met Tyr Arg
35 40 45
Ser Ala Lys Lys Tyr Ala Phe Ser Phe Leu Gly Asp Ile Pro Leu Ile
50 55 60
Thr Tyr Asn Gly Ala Leu Ile Lys Tyr Ser Arg Ser Glu Arg Glu Ile
65 70 75 80
Tyr His Gln Pro Val Pro Gly Glu Leu Ala Leu Thr Ile Tyr Arg Arg
85 90 95
Val Lys Gly His Phe His Leu Asn Val Tyr Gln Asp Asp Glu Leu Phe
100 105 110
Val Glu Glu Asp Asn Gln Tyr Ile Arg Asp Tyr Ser Lys Ile Ala Gly
115 120 125
Val Pro Phe Arg Val Val Asn Asn Ile Glu Glu Leu Leu Gly Lys Lys
130 135 140
Ala Pro Thr Lys Leu Leu Ala Ile Gly Asp Pro Glu Glu Leu Asp Gln
145 150 155 160
Leu Trp Glu Glu Thr Asn Gly Glu Phe Arg Gly Val Leu His Ile Thr
165 170 175
Lys Ser Lys Pro His Tyr Leu Glu Phe Leu Ala Ala Gly Val Asn Lys
180 185 190
Gly Glu Ala Leu Lys Ile Leu Ala Gln His Leu Arg Ile Ser Leu Lys
195 200 205
Glu Thr Val Ala Val Gly Asp Ser Tyr Asn Asp Leu Glu Met Leu Glu
210 215 220
Ala Ala Gly Leu Gly Val Ala Met Gly Asn Ala Leu Pro Glu Val Lys
225 230 235 240
Arg Arg Ala Asp Leu Val Val Pro Ala Asn Asp Glu Asp Gly Ile Ala
245 250 255
Tyr Leu Ile Asn Glu Ile Ile Leu Lys Asn
260 265
<210> 34
<211> 231
<212> PRT
<213> Carboxydothermus ferrireducens
<400> 34
Met Ala Tyr Ile Leu Phe Asp Leu Asp Gly Thr Leu Leu Pro Leu Asn
1 5 10 15
Leu Asp Tyr Phe Leu Lys Leu Tyr Ile Gln Glu Val Lys Ser Tyr Val
20 25 30
Lys Gly Lys Phe Asp Pro Asp Leu Ile Val Lys Ala Ile Leu Lys Ala
35 40 45
Thr Glu Glu Met Val Lys Asn Thr Gly Glu Lys Leu Asn Ser Glu Val
50 55 60
Phe Trp Gln Ala Phe Asp Arg Val Tyr Pro Gly Thr Ala Asn Leu His
65 70 75 80
Asn Val Phe Glu Asp Phe Tyr His Thr Ser Phe Lys Asn Ile Gly Asn
85 90 95
Tyr Phe Lys Pro His Pro Leu Ala Arg Pro Met Ile Arg Tyr Leu His
100 105 110
Lys Lys Gly His Thr Leu Ile Leu Ala Thr Asn Ala Leu Phe Pro Arg
115 120 125
Ser Ala Ile Ile Glu Arg Leu Tyr Trp Ala Asn Leu Ser Pro Lys Tyr
130 135 140
Phe Arg Phe Ile Thr His Tyr Asp Asn Met His Tyr Cys Lys Pro Asn
145 150 155 160
Pro Asn Tyr Tyr Lys Glu Ile Leu Glu Lys Ile Lys Ala Arg Pro Ser
165 170 175
Asp Cys Leu Met Ile Gly Asn Asp Pro Ala Glu Asp Leu Val Ala Lys
180 185 190
Glu Leu Gly Ile Lys Thr Phe Leu Val Lys Asp Tyr Glu Val Lys Arg
195 200 205
Glu Asn Pro Arg Thr Pro Asp Tyr Ala Gly Asp Phe Tyr Glu Leu Tyr
210 215 220
Arg Phe Ile Arg Glu Asn Phe
225 230
<210> 35
<211> 212
<212> PRT
<213> Carboxydothermus ferrireducens
<400> 35
Met Ile Lys Ala Val Phe Phe Asp Leu Asp Gly Thr Leu Leu Asp Thr
1 5 10 15
Phe Asp Leu Ile Tyr Glu Ser Phe Lys His Val Tyr Lys Asn Phe Leu
20 25 30
Asn Lys Asp Ile Thr Arg Glu Glu Ile Tyr Pro Tyr Phe Gly Lys Pro
35 40 45
Leu Ile Tyr Ser Phe Glu Asn Leu Asp Pro Glu Thr Ile Asp Gln Val
50 55 60
Ile Ala Ala Tyr Arg Glu Phe Asn Leu Gln His His Asp Gln Met Val
65 70 75 80
Lys Pro Phe Pro Gly Ala Lys Glu Thr Leu Glu Lys Leu Lys Gln Arg
85 90 95
Gly Lys Ile Leu Ala Val Ile Thr Ser Lys Val Lys Ser Thr Ala Ile
100 105 110
Arg Gly Leu Lys Leu Phe Asn Leu Asp Arg Tyr Phe Asp Leu Val Val
115 120 125
Ala Leu Glu Asp Thr Glu Lys His Lys Pro Asp Pro Ala Pro Val Leu
130 135 140
Tyr Ala Leu Lys Phe Phe Gln Val Lys Pro Glu Gln Cys Leu Met Val
145 150 155 160
Gly Asp Ser Pro His Asp Met Val Ser Ala Gln Arg Ala Gly Val Lys
165 170 175
Thr Ala Ala Val Lys Trp Ser Val Leu Pro Trp Glu Asp Leu Val Lys
180 185 190
Thr Lys Pro Asn Tyr Ile Leu Asn Ser Phe Asp Asp Leu Leu Lys Ile
195 200 205
Thr Gly Val Glu
210
<210> 36
<211> 218
<212> PRT
<213> 绿弯菌门细菌54-19(Chloroflexi bacterium 54-19)
<400> 36
Met Asn Lys Phe Gln Gly Val Ile Leu Asp Ile Asp Gly Thr Leu Ile
1 5 10 15
Asp Ser Asn Asp Ala His Ala His Ser Trp Val Asp Thr Phe Ala Glu
20 25 30
Ala Gly Ile Pro Val Pro Phe Glu Arg Val Arg Pro Leu Ile Gly Glu
35 40 45
Gly Gly Asp Lys Leu Leu Pro Glu Val Ser Gly Phe Glu Ala Gly Ser
50 55 60
Ala Glu Gly Lys Arg Leu Thr Lys Arg Arg Thr Glu Ile Phe Lys Glu
65 70 75 80
Lys Tyr Leu Pro His Ile Gln Pro Phe Pro Lys Thr Arg Glu Leu Ile
85 90 95
Thr Ala Phe Glu Lys Arg Gly Leu Lys Leu Gln Ile Ala Thr Ser Ala
100 105 110
Gln Glu Ala Glu Val Lys Asp Leu Leu Lys Val Ala Gly Thr Pro Glu
115 120 125
Ile Ile Glu Gln Lys Thr Thr Ser Asp Asp Ala Glu Lys Ser Lys Pro
130 135 140
Asp Pro Asp Ile Ile His Ala Ala Leu Gln Lys Ile Gly Leu Glu Ala
145 150 155 160
Gly Gln Val Val Met Leu Gly Asp Thr Pro Tyr Asp Val Ala Ala Ala
165 170 175
Lys Lys Ala Gly Val Lys Ser Tyr Ala Phe Arg Cys Gly Gly Trp Trp
180 185 190
Gln Asp Arg Asp Phe Glu Asp Ala Ala Ala Ile Phe Asp Gly Pro Ala
195 200 205
Asp Leu Leu Glu Lys Leu Asp Asp Tyr Leu
210 215
<210> 37
<211> 284
<212> PRT
<213> 突尼斯废水袍菌(Defluviitoga tunisiensis)
<400> 37
Met Asn Tyr His Leu Asn Ser Ser Thr Lys Val Phe Val Phe Asp Phe
1 5 10 15
Asp Gly Thr Leu Leu Asn Ser Glu Val Arg Ile Ser Pro Arg Thr Phe
20 25 30
Glu Ala Leu Lys Lys Leu Lys Glu His Gly His Thr Ile Ile Leu Cys
35 40 45
Ser Gly Arg Met Tyr Ala Ser Met Leu Phe Ile Val Glu Asn Phe Leu
50 55 60
Pro Phe Leu Lys Gly Tyr Ala His Ile Val Ser Tyr Asn Gly Gly Tyr
65 70 75 80
Ile Val Asn Asn Lys Gly Glu Leu Leu Phe Glu Glu Gly Leu Asp Lys
85 90 95
Asp Val Ala Ile Asp Cys Ile Glu Phe Leu Arg Glu Leu Asn Val His
100 105 110
Arg His Val Tyr Ile Asn Asp Glu Leu Ile Ser Glu Gln Asp Asp Gln
115 120 125
Glu Ile Lys Asp Tyr Ser Lys His Ser Phe Val Ala Tyr Lys Leu Val
130 135 140
Asp Asp Leu Val Asp Thr Ile Lys Asn Ser Glu His Pro Thr Leu Lys
145 150 155 160
Ile Leu Gly Ile Cys Glu Gln Glu Lys Leu Asn Met Val Gln Ser Leu
165 170 175
Ala Glu Lys Lys Phe Glu Gly Lys Ile Asn Ile Met Arg Ser Phe Ser
180 185 190
Thr Tyr Leu Asp Phe Met Pro Phe Gly Val Ser Lys Gly Glu Ala Leu
195 200 205
Lys Ile Leu Ser Lys Ile Tyr Asn Phe Asn Val Glu Glu Val Tyr Val
210 215 220
Phe Gly Asp Ser Gln Asn Asp Ile Asp Met Leu Arg Ile Ser Arg Asn
225 230 235 240
Ser Phe Ala Met Gly Asn Ala Gln Lys Asp Val Lys Glu Val Ala Arg
245 250 255
Tyr Val Ile Pro Ser Asn Asp Glu Asp Gly Val Ala Phe Ala Ile Glu
260 265 270
Lys Ile Leu Ser Asp Asp Leu Asp Asn Val Asn Ile
275 280
<210> 38
<211> 231
<212> PRT
<213> Deinococcus aerius
<400> 38
Met Thr Val Pro Gln Pro Thr Asp Pro Ala Thr Val Leu Asn Pro Glu
1 5 10 15
Arg Leu Arg Gly Val Leu Leu Asp Val Asp Gly Thr Leu Ile Asp Ser
20 25 30
Asn Asp Ala His Ala Arg Ala Trp Val Glu Ala Leu Arg Glu Glu Gly
35 40 45
Phe Glu Arg Ser Phe Gly Glu Val Arg Pro Leu Ile Gly Met Gly Gly
50 55 60
Asp Gln Leu Val Pro Arg Leu Thr Gly Glu Asp Gly Glu Ser Glu Val
65 70 75 80
Gly Lys Arg Leu Thr Gln Gly Trp Leu Lys His Phe Lys Pro Leu Val
85 90 95
Pro Gly Leu Arg Pro Thr Arg Gly Asn Arg Ala Leu Ile Glu Gly Leu
100 105 110
Arg Ser Arg Gly Leu Lys Val Val Leu Ala Thr Ser Gly Glu Ala Glu
115 120 125
Ile Val Asp Ser Leu Leu Lys Gln Ala Asn Leu Gly Asp Leu Asn Leu
130 135 140
Asp Arg Val Ser Ser Ser Glu Val Gly Ser Ser Lys Pro Ala Pro Asp
145 150 155 160
Leu Val Gln Val Gly Leu Asp Lys Leu Gly Leu Pro Ala Gly Ala Ala
165 170 175
Leu Met Val Gly Asp Thr Pro Phe Asp Ala Glu Ala Ala His Gly Ala
180 185 190
Gly Val Pro Cys Ala Leu Leu Arg Cys Gly Gly Asp Ser Glu Glu Glu
195 200 205
Leu Gly Arg Thr Gly Ala Leu Val Leu Asn Asp Pro Gln Ala Leu Leu
210 215 220
Glu Ala Leu Glu Gly Ala Ser
225 230
<210> 39
<211> 231
<212> PRT
<213> Deinococcus apachensis
<400> 39
Met Thr Ala Leu Gln Pro Ala Asp Pro Ala Ala Ala Leu Asn Pro Asp
1 5 10 15
Arg Leu Arg Gly Val Leu Leu Asp Val Asp Gly Thr Leu Ile Asp Ser
20 25 30
Asn Asp Ala His Ala Arg Ala Trp Val Glu Ala Leu Arg Glu Glu Gly
35 40 45
Phe Glu Arg Ser Phe Gly Glu Val Arg Pro Leu Ile Gly Met Gly Gly
50 55 60
Asp Gln Leu Ile Pro Arg Leu Thr Gly Glu Asp Gly Glu Ser Glu Leu
65 70 75 80
Gly Lys Arg Leu Thr Gln Gly Trp Leu Lys His Phe Lys Pro Leu Ile
85 90 95
Pro Glu Leu Gln Pro Thr Arg Gly Asn Arg Ala Leu Ile Glu Gly Leu
100 105 110
Arg Ala Arg Gly Leu Arg Val Ala Leu Ala Thr Ser Gly Glu Ala Glu
115 120 125
Ile Val Asp Gly Leu Leu Lys Gln Ala Gly Leu Asp Asp Leu Asn Leu
130 135 140
Asp Arg Val Ser Ser Ser Glu Val Glu Ser Ser Lys Pro Glu Pro Asp
145 150 155 160
Leu Val Glu Ala Gly Leu Asn Lys Leu Gly Leu Pro Ala Asp Ala Ala
165 170 175
Leu Met Val Gly Asp Thr Pro Phe Asp Ala Glu Ala Ala His Lys Ala
180 185 190
Gly Val Pro Cys Val Leu Leu Arg Cys Gly Gly Asp Ser Asp Glu Glu
195 200 205
Leu Gly Arg Ala Pro Ala Leu Val Leu Asp Asp Pro Gln Ala Leu Leu
210 215 220
Glu Ala Leu Glu Gly Thr Ser
225 230
<210> 40
<211> 228
<212> PRT
<213> Deinococcus aquatilis
<400> 40
Met Arg Arg Ala Glu Asp Val Tyr Leu Asp Arg Met Tyr Lys Gly Val
1 5 10 15
Ile Phe Asp Ile Asp Gly Thr Leu Val Asp Ser Asn Asp Ala His Ala
20 25 30
Arg Ala Trp Val Lys Ala Phe Ala Asp Glu His Ile Thr Val Pro Phe
35 40 45
Asp Gln Val Arg Pro Leu Ile Gly Met Gly Ser Asp Gln Met Val Pro
50 55 60
Arg Leu Thr Asp Val Gln Lys Asp Ser Pro Thr Phe Lys Arg Leu Gly
65 70 75 80
Glu Ala Trp Lys Gln His Phe Gln Ala Glu Glu Met Pro His Leu Lys
85 90 95
Ala Gln Pro Gly Val Arg Ala Leu Ile Glu Arg Leu Gln Glu Arg Gly
100 105 110
Leu Arg Leu Ile Val Gly Thr Ser Ala Asp Glu Ala Leu Val Ala Asp
115 120 125
Leu Leu Arg Val Ala Gly Val Asp Asp Leu Leu Thr Glu Tyr Thr Thr
130 135 140
Ala Ser Asp Val Glu Ser Ser Lys Pro Glu Pro Asp Ile Val Gln Ala
145 150 155 160
Ala Val Thr Lys Leu Gly Leu Ala Pro Ala Glu Val Leu Met Val Gly
165 170 175
Asp Thr Pro Phe Asp Ile Glu Ser Ala His Lys Ala Gly Val Ala Thr
180 185 190
Val Ala Leu Arg Cys Gly Gly Asp Thr Arg Phe Glu Gly Ala Ala Ala
195 200 205
Val Tyr Ala Asp Pro Gln Glu Trp Leu Glu Gln Leu Asp Gln Ser Pro
210 215 220
Leu Gly Lys Gly
225
<210> 41
<211> 229
<212> PRT
<213> Deinococcus geothermalis
<400> 41
Met Ser His Pro Glu Pro Thr Ala Pro Ala Ala Ala Leu Asp Pro Lys
1 5 10 15
Arg Leu Arg Gly Val Leu Leu Asp Val Asp Gly Thr Leu Ile Asp Ser
20 25 30
Asn Asp Ala His Ala Arg Ala Trp Val Glu Ala Leu Arg Glu Ala Gly
35 40 45
Phe Glu Arg Thr Phe Glu Glu Val Arg Pro Leu Ile Gly Met Gly Gly
50 55 60
Asp Gln Leu Ile Pro Arg Leu Thr Gly Leu Asp Ser Gln Ser Glu Glu
65 70 75 80
Gly Gln Arg Leu Thr Gln Gly Trp Leu Arg His Phe Lys Pro Leu Ile
85 90 95
Pro Thr Leu His Ala Thr Arg Gly Ala Arg Ala Leu Val Gln Gly Leu
100 105 110
His Ala Arg Gly Leu Gln Val Leu Leu Ala Thr Ser Gly Glu Ala Glu
115 120 125
Ile Val Asp Glu Leu Leu Lys Gln Ala His Leu Asp Asp Leu Gln Leu
130 135 140
Glu Arg Val Ser Ser Ser Glu Val Glu Ser Ser Lys Pro Ala Pro Asp
145 150 155 160
Leu Val Gln Ala Gly Leu Glu Lys Leu Gly Leu Pro Ala Gly Glu Ala
165 170 175
Leu Leu Val Gly Asp Thr Pro Tyr Asp Ala Gln Ala Ala His Lys Ala
180 185 190
Gly Val Pro Cys Val Leu Leu Arg Cys Gly Gly Asn Thr Gly Leu Glu
195 200 205
Glu His Ala Pro Thr Leu Asp Asp Pro Gln Ala Leu Leu Glu Val Leu
210 215 220
Glu Gly Thr Lys Gly
225
<210> 42
<211> 225
<212> PRT
<213> Deinococcus hopiensis
<400> 42
Met Ser Leu Lys Asp Leu Leu Ser Pro Ser His Leu Arg Gly Val Leu
1 5 10 15
Leu Asp Val Asp Gly Thr Leu Ile Asp Ser Asn Gly Ala His Ala Arg
20 25 30
Ala Trp Val Gln Ala Leu Arg Asp Glu Gly Phe Ala Arg Thr Phe Glu
35 40 45
Asp Val Arg Pro Leu Ile Gly Met Gly Gly Asp Lys Leu Val Pro Glu
50 55 60
Leu Thr Gly Glu Asp Pro Glu Gly Glu Arg Ala Lys Arg Met Lys Asp
65 70 75 80
Ala Trp Leu Lys His Phe Gln Pro Met Ile Pro Lys Leu Gln Pro Thr
85 90 95
Arg Gly Ala Arg Glu Met Ile Glu Gly Leu Leu Ala Arg Asp Leu Arg
100 105 110
Val Ala Ile Ala Thr Ser Gly Glu Ala Glu Ile Val Glu Gly Leu Leu
115 120 125
Ala Arg Val Gly Val Ala His Leu Lys Leu Asp Arg Val Ser Ser Ser
130 135 140
Glu Val Asp His Ser Lys Pro Asp Pro Asp Leu Ile Gln Val Gly Leu
145 150 155 160
Asn Lys Leu Gly Val Ser Ala Glu Gln Ala Leu Met Val Gly Asp Thr
165 170 175
Pro Phe Asp Ala Glu Ala Ala Arg Lys Ala Gly Val Pro Ser Val Leu
180 185 190
Leu Arg Cys Gly Gly Asp Ala Arg Val Glu Gln His Ala Tyr Val Leu
195 200 205
Asp Asp Pro Arg Ala Leu Leu Glu Ala Leu Pro Asp Gly Ser Gln Thr
210 215 220
Val
225
<210> 43
<211> 214
<212> PRT
<213> Deinococcus maricopensis
<400> 43
Met Ser Ile Arg Ala Val Ile Met Asp Leu Asp Gly Thr Leu Val Asp
1 5 10 15
Ser Asn Asp Ala His Ala Arg Ala Trp Val Ala Ala Leu Arg Asp Phe
20 25 30
Gly Ile Glu Arg Thr Phe Glu Asp Val Arg Pro Leu Ile Gly Met Gly
35 40 45
Gly Asp Gln Leu Leu Pro Arg Val Ala Asp Val Asp Pro Asn Ser Glu
50 55 60
Arg Gly Glu Gly Leu Thr Gln Ala Trp Ala Glu His Phe Lys Pro Met
65 70 75 80
Ile Pro Glu Leu Arg Ala Thr Arg Gly Ala Arg Glu Leu Leu Glu Ala
85 90 95
Leu His Gly Arg Gly Leu Arg Val Ile Leu Gly Thr Ser Gly Asp Ser
100 105 110
Asp Val Ile Asp Pro Leu Leu Glu His Ile Gly Val Arg Asp Leu Val
115 120 125
Pro Asp Arg Val Thr Ala Asp Asp Val Glu Ala Ser Lys Pro Glu Pro
130 135 140
Asp Ile Leu His Ala Ala Leu Gln Lys Leu Gly Val Gly Ala His Glu
145 150 155 160
Ala Leu Met Val Gly Asp Thr Pro Phe Asp Ala Glu Ala Ala Lys Arg
165 170 175
Ala Asn Val Arg Val Ala Leu Leu Arg Ala Gly Gly Asp Glu Arg Val
180 185 190
Asn Ala Glu Ala Phe Thr Phe Asp Asp Pro Ala Asp Leu Ala Ala His
195 200 205
Leu Asp Asp Val Leu Arg
210
<210> 44
<211> 261
<212> PRT
<213> Deinococcus murrayi
<400> 44
Met Leu Leu Ala Phe Asp Leu Asp Gly Thr Leu Ile Pro Asp Leu Gly
1 5 10 15
Arg Glu Val Pro Ala Ala Thr Val Arg Ala Leu Ala Arg Leu Arg Gly
20 25 30
Leu Gly Ala Arg Val Ala Val Ile Thr Gly Arg Asp Ala Ala Pro Pro
35 40 45
Gln Val Leu Glu Ala Ala Gln Pro Asp Ala Val Ala Thr Asn Asn Gly
50 55 60
Gly Arg Val Glu Leu Gln Gly Glu Leu His Arg Glu Ala Arg Phe Ser
65 70 75 80
Glu Asp Glu Leu Ala Ala Val Leu Ala His Glu Leu Glu Glu Ala Arg
85 90 95
Val Val Val Phe Arg Pro Ser Gly Leu Tyr Ala Glu Leu Pro Pro Gly
100 105 110
His Ala Pro Glu Ala Trp Met Val Glu Arg Gly Val Arg Pro Leu Ala
115 120 125
Gln Ala Pro Ala Gly Glu Pro Thr Leu Lys Val Gly Phe Tyr His Pro
130 135 140
Gly Val Ala Asp Phe Ala Ala Arg Leu Arg Val Ser His Pro His Leu
145 150 155 160
Val Leu Thr Gly Ala Gln Pro Pro Tyr Ser Glu Phe Leu Thr Val Thr
165 170 175
Pro Ala Gly Ala His Lys Gly Ala Ala Leu Thr Leu Ile Ala Gln Gly
180 185 190
Leu Gly Val Glu Leu Glu Arg Thr Val Val Phe Gly Asp Ser Asp Asn
195 200 205
Asp Val Ala Met Leu Glu Ile Ala Gly Tyr Ala Val Gln Val Gly His
210 215 220
Leu Pro Leu Leu Ala Pro His Ala Asp Asp Arg Val Ser Gly Pro Gln
225 230 235 240
Ala Leu Gly Pro Tyr Leu Glu Glu Leu Ala Asp Arg Leu Glu Ala Ala
245 250 255
Glu Ala Pro Ala Thr
260
<210> 45
<211> 225
<212> PRT
<213> Deinococcus murrayi
<400> 45
Met Thr His His Asp Leu Leu Ser Pro Glu Arg Leu Arg Gly Val Leu
1 5 10 15
Leu Asp Val Asp Gly Thr Leu Ala Asp Ser Asn His Ala His Ala Arg
20 25 30
Ala Trp Ala Ala Ala Leu Arg Asp Glu Gly Phe Glu Lys Ser Pro Glu
35 40 45
Ala Ile Phe Pro Leu Ile Gly Met Gly Gly Asp Lys Leu Val Pro Glu
50 55 60
Leu Thr Gly Leu Asp Ala Glu Ser Glu Arg Gly Gln Arg Met Thr Glu
65 70 75 80
Gly Trp Val Arg His Phe Gln Glu Leu Ile Pro Asp Leu Arg Pro Thr
85 90 95
Pro Gly Ala Arg Ala Leu Val Glu Gly Leu Arg Ala Arg Gly Leu Arg
100 105 110
Val Val Leu Ala Thr Ser Gly Glu Ala Glu Val Val Asp Ala Leu Leu
115 120 125
Arg Gln Ile Gly Leu Asp Asp Leu Glu Leu Glu Arg Val Ser Ser Thr
130 135 140
Asp Val Glu Asn Ser Lys Pro Asp Pro Asp Leu Val Gln Ala Gly Leu
145 150 155 160
Arg Thr Leu Gly Leu Pro Ala Glu Ala Thr Leu Met Val Gly Asp Thr
165 170 175
Pro Tyr Asp Ala Glu Ala Ala Arg Lys Ala Gly Val Pro Cys Val Leu
180 185 190
Leu Arg Cys Gly Gly His Pro Gly Val Glu Glu His Asp Pro Val Tyr
195 200 205
Asp His Pro Gln Ala Leu Leu Glu Ala Leu Asp Gln Ala Phe Pro Val
210 215 220
Glu
225
<210> 46
<211> 229
<212> PRT
<213> Deinococcus reticulitermitis
<400> 46
Met Ala Asp Leu Gln Leu Ser Ser Pro Ala Leu Pro Ala Val Ser Arg
1 5 10 15
Leu Lys Gly Leu Leu Leu Asp Leu Asp Gly Thr Leu Val Asp Ser Asn
20 25 30
Gly Ala His Thr Glu Ala Trp Val Gln Ala Leu Ala Asp Gln Gly Phe
35 40 45
Thr Arg Pro Pro Ala Glu Val Arg Pro Leu Ile Gly Met Gly Gly Asp
50 55 60
Gln Leu Val Pro Arg Leu Thr Gly Leu Asp Glu Gly Ser Glu Thr Gly
65 70 75 80
Lys Ala Leu Val Glu Gly Trp Gln Thr His Phe Lys Ser Arg Met Pro
85 90 95
Ser Val Arg Thr Phe Pro Gly Ala Arg Glu Leu Leu Glu Trp Ala Arg
100 105 110
Glu Val Gly Leu Pro Val Val Leu Ala Ser Ser Gly Glu Asp Glu Ile
115 120 125
Val Glu Gly Leu Leu Ala Gln Ala Gly Leu Leu Glu Leu Val Thr Asp
130 135 140
Arg Val Arg Ser Asp Glu Val Asn Gln Thr Lys Pro Arg Pro Asp Val
145 150 155 160
Val Gln Ala Ala Leu Lys Lys Ala Gly Val Ala Pro Glu Glu Ala Leu
165 170 175
Phe Val Gly Asp Thr Val His Asp Ala Arg Ala Ala Arg Ala Ala Gly
180 185 190
Val Pro Cys Val Leu Val Arg Ala Gly Gly Ser Pro Gly Leu Asp Ala
195 200 205
Glu Pro His Val Leu Gly Asp Leu Met Glu Leu Leu Ala Ala Leu Lys
210 215 220
Ala Gly Ser Thr Thr
225
<210> 47
<211> 227
<212> PRT
<213> Deinococcus wulumuqiensis
<400> 47
Met Arg Leu Ser Pro Gln Thr Pro Ala Leu Pro Ala Val Pro Asp Leu
1 5 10 15
Arg Ala Leu Leu Leu Asp Leu Asp Gly Thr Leu Ala Asp Ser Asn Asp
20 25 30
Ala His Ala Arg Ala Trp Val Gln Ala Leu Ala Asp Gln Asp Ile Thr
35 40 45
Arg Glu Glu Ser Glu Val Arg Pro Leu Ile Gly Met Gly Gly Asp Gln
50 55 60
Leu Val Pro Ala Leu Thr Gly Glu Ser Asp Arg Ser Glu Leu Gly Lys
65 70 75 80
Ala Leu Val Gln Gly Trp Gln Asp His Phe Gly Pro Met Ile Pro Glu
85 90 95
Ile Ala Pro Leu Gly Gly Ala Arg Glu Leu Leu Glu Trp Ala Arg Thr
100 105 110
Gln Gly Leu Lys Val Val Leu Ala Ser Ser Gly Glu Asp Asp Ile Val
115 120 125
Asp Ala Leu Leu Glu Gln Ile Gly Val Gly Asp Leu Val Pro Leu Arg
130 135 140
Val Arg Ser Asp Glu Val Gln Arg Thr Lys Pro Ala Pro Asp Val Leu
145 150 155 160
Gln Ala Ala Leu Ala Lys Ala Gly Val Ser Ala Ala Gln Ala Leu Phe
165 170 175
Val Gly Asp Thr Thr Tyr Asp Ala Arg Ala Ala Arg Ala Ala Gly Val
180 185 190
Ala Cys Val Val Leu Arg Ala Gly Gly Ser Pro Gly Leu Asn Thr Glu
195 200 205
Pro His Val Leu Ser Asp Pro Ala Glu Leu Leu Ala Glu Leu Lys Lys
210 215 220
Ala Thr Ser
225
<210> 48
<211> 224
<212> PRT
<213> Deinococcus sp. Leaf326
<400> 48
Met Ser Ser Tyr Leu Gly Val Ile Phe Asp Leu Asp Gly Thr Leu Val
1 5 10 15
Asp Ser Asn Asp Gly His Ala Arg Ala Trp Val Arg Ala Phe Ala Asp
20 25 30
Gln Gly Ile Glu Val Thr Phe Gly Gln Val Arg Pro Leu Met Gly Met
35 40 45
Gly Gly Asp Gln Leu Val Pro His Leu Thr Gly Ile Gly Lys Asp Asp
50 55 60
Pro Arg Tyr Glu Ala Leu Ser Asp Gly Trp Lys Arg His Phe Gln Ala
65 70 75 80
Glu Glu Leu Pro Arg Leu Arg Ala Gln Pro Gly Val Arg Pro Leu Leu
85 90 95
Val Ala Leu Gln Ala Gln Gly Leu Arg Leu Ile Val Gly Thr Ser Ala
100 105 110
Asp Glu Ala Leu Val Arg Asp Leu Leu Gly Val Ala Gly Ala Gln Asp
115 120 125
Leu Phe Thr Glu Tyr Thr Thr Ala Ser Glu Val Glu Glu Ser Lys Pro
130 135 140
Gln Pro Asp Ile Val Gln Ala Ala Val Arg Lys Leu Gly Leu Asp Pro
145 150 155 160
Ala Gln Val Leu Met Val Gly Asp Thr Ser Phe Asp Ile Glu Ser Ala
165 170 175
Arg Gly Ala Gly Val Ala Thr Val Ala Leu Arg Ser Gly Gly Thr Ala
180 185 190
Gln Phe Thr Gly Ala Ala Ala Val Tyr Asp Ser Pro Gln Asp Trp Leu
195 200 205
Asp His Leu Ala Ala Ser Pro Leu Gly Pro Asp Gln Pro Ser Ala Ser
210 215 220
<210> 49
<211> 225
<212> PRT
<213> Deinococcus phoenicis
<400> 49
Met Thr Pro Ser Leu Glu Ala Ala Leu Asn Pro Asp Arg Leu Arg Gly
1 5 10 15
Val Leu Leu Asp Val Asp Gly Thr Leu Ile Asp Ser Asn Asp Ala His
20 25 30
Ala Arg Ala Trp Val Glu Ala Leu Arg Asp Glu Gly Phe Glu Lys Thr
35 40 45
Val Gly Glu Val Arg Pro Leu Ile Gly Met Gly Gly Asp Gln Met Ile
50 55 60
Pro Arg Leu Thr Gly Lys Gly Ser Glu Ser Glu Val Gly Gln Arg Leu
65 70 75 80
Thr Asp Gly Trp Leu Lys His Phe Lys Pro Leu Ile Pro Glu Leu His
85 90 95
Ala Thr Arg Gly Ala Arg Ala Leu Val Glu Gly Leu Arg Ala Arg Gly
100 105 110
Leu Arg Val Val Leu Ala Thr Ser Gly Glu Ala Glu Val Val Asp Ala
115 120 125
Leu Leu Lys Gln Ala Gly Leu Ala Asp Leu Asn Leu Asp Arg Val Ser
130 135 140
Ser Ser Glu Val Glu Ser Ser Lys Pro Ala Pro Asp Leu Ile Gln Val
145 150 155 160
Gly Leu Lys Lys Leu Gly Val Pro Ala Glu Ala Ala Leu Met Val Gly
165 170 175
Asp Thr Pro Tyr Asp Ala Glu Ala Ala Ala Lys Ala Asp Val Pro Cys
180 185 190
Ile Leu Leu Arg Cys Gly Gly Asp Thr Asp Leu Glu Lys His Ala Pro
195 200 205
Val Leu Asp Asp Pro Gln Ala Leu Leu Glu Ala Leu Glu Lys Ala Thr
210 215 220
Val
225
<210> 50
<211> 228
<212> PRT
<213> Deinococcus proteolyticus
<400> 50
Met Gln Leu Asn Pro Gln Gly Pro Ser Leu Pro Thr Ile Ala Asp Leu
1 5 10 15
Arg Ala Leu Phe Val Asp Leu Asp Gly Thr Leu Val Asp Ser Asn Asp
20 25 30
Ala His Ala Arg Ala Trp Val Gln Ala Leu Ala Asp Gln Asp Ile Thr
35 40 45
Arg Asp Gln Gly Glu Val Arg Pro Leu Ile Gly Met Gly Gly Asp Gln
50 55 60
Leu Val Pro Glu Leu Thr Gly Lys Ser Asp Ser Ser Glu Leu Gly Glu
65 70 75 80
Ala Leu Val Gln Gly Trp Gln Asp His Phe Lys Pro Leu Ile Ala Gly
85 90 95
Leu Glu Val Leu Pro Gly Ala Arg Glu Leu Leu Glu Trp Ala Arg Gly
100 105 110
Gln Gly Leu Thr Val Val Leu Ala Ser Ser Gly Glu Asp Asp Ile Val
115 120 125
Glu Ala Leu Leu Glu His Ala Gly Leu Thr Asp Leu Ile Asp Leu Arg
130 135 140
Val Arg Ser Asp Glu Val Gln Gln Thr Lys Pro Glu Pro Asp Ile Leu
145 150 155 160
Gln Ala Ala Leu Asn Lys Ala Gly Val Gln Pro Gln Gln Ala Leu Phe
165 170 175
Val Gly Asp Thr Arg Phe Asp Ala Glu Ala Ala Gln Lys Ala Gly Val
180 185 190
Pro Cys Val Leu Leu Arg Ala Gly Gly Ser Pro Asp Leu Glu Glu Ala
195 200 205
Gln Tyr Val Leu Ala Asn Ala His Glu Leu Leu Ser Ala Leu Gln Ala
210 215 220
Ala Thr Gln Asp
225
<210> 51
<211> 216
<212> PRT
<213> Deinococcus sp. 17bor-2
<400> 51
Met Thr Thr Ser Leu Gln Ala Ala Ile Leu Asp Leu Asp Gly Thr Leu
1 5 10 15
Ile Asp Ser Asn Asp Ala His Ala Arg Ala Trp Val Thr Ser Leu Lys
20 25 30
Arg His Gly Phe Asp Ile Pro Phe Glu Arg Val Arg Pro Leu Ile Gly
35 40 45
Met Gly Gly Asp Lys Leu Ile Pro Glu Leu Thr Gly Leu Asp Pro Glu
50 55 60
Ser Glu Thr Gly Lys Ala Leu Thr Gln Gly Trp Gly Asp Ala Phe Lys
65 70 75 80
Pro Met Ile Pro Ser Leu Gln Ala Thr Pro Gly Ala Arg Glu Leu Val
85 90 95
Ala Gly Leu Arg Glu Leu Gly Trp Lys Leu Met Leu Gly Ser Ser Gly
100 105 110
Glu Asp Glu Val Val Glu Gln Glu Leu Glu His Leu Gly Leu Glu Asp
115 120 125
Leu Lys Asn Ser Arg Val Thr Ser Ser Glu Val Glu Glu Ser Lys Pro
130 135 140
Asp Ala Asp Ile Leu Ala Val Ala Leu Arg Lys Leu Gly Val Pro Ala
145 150 155 160
Gly Ala Ala Leu Met Val Gly Asp Thr Lys Tyr Asp Ala Glu Ala Ala
165 170 175
Arg Arg Ala Gly Val Arg Cys Val Leu Leu Arg Cys Gly Gly Asn Pro
180 185 190
Asp Leu Pro Gly Glu Val Tyr Ala Ser Pro Ala Asp Leu Leu Gly Ala
195 200 205
Leu Arg Ser Gly Asp Trp Gly Arg
210 215
<210> 52
<211> 225
<212> PRT
<213> Deinococcus sp. NW-56
<400> 52
Met Thr Gln His Asp Leu Leu Ala Pro Asp Arg Leu Arg Gly Val Leu
1 5 10 15
Leu Asp Val Asp Gly Thr Leu Ala Asp Ser Asn Leu Ala His Ala Arg
20 25 30
Ala Trp Ala Ala Ala Leu Ala Asp Glu Gly Phe Glu Lys Thr Pro Glu
35 40 45
Asp Ile Phe Pro Leu Ile Gly Met Gly Gly Asp Lys Leu Val Pro Glu
50 55 60
Leu Thr Gly Leu Asp Ala Glu Ser Glu Arg Gly Gln Arg Leu Thr Asp
65 70 75 80
Gly Trp Val Arg His Phe Arg Glu Leu Ile Pro Asp Leu Arg Pro Thr
85 90 95
Pro Gly Ala Arg Ala Leu Ile Glu Gly Leu Arg Ala Arg Gly Leu Arg
100 105 110
Val Ala Leu Ala Thr Ser Gly Glu Ala Glu Ile Val Asp Ala Leu Leu
115 120 125
Glu Gln Ile Gly Leu Asp Asp Leu Lys Leu Glu Arg Val Ser Ser Ser
130 135 140
Asp Val Glu Asn Ser Lys Pro Asp Pro Asp Leu Val Lys Ala Gly Leu
145 150 155 160
His Thr Leu Gly Leu Pro Ala Gly Ala Ala Leu Met Val Gly Asp Thr
165 170 175
Pro Tyr Asp Ala Glu Ala Ala Arg Gly Ala Gly Val Pro Cys Val Leu
180 185 190
Leu Arg Cys Gly Gly His Pro Gly Ala Glu Gly His Asp Pro Leu Tyr
195 200 205
Asp Asp Pro Gln Ala Leu Leu Ala Ala Leu Asn Glu Ala Phe Pro Val
210 215 220
Glu
225
<210> 53
<211> 225
<212> PRT
<213> Deinococcus sp. RL
<400> 53
Met Thr Gln Gly Asp Leu Leu Ser Pro Lys Arg Leu Arg Gly Val Leu
1 5 10 15
Leu Asp Val Asp Gly Thr Leu Ala Asp Ser Asn Met Ala His Ala Arg
20 25 30
Ala Trp Ala Ala Ala Leu Arg Asp Glu Gly Phe Glu Gln Ser Pro Glu
35 40 45
Ala Ile Leu Pro Leu Ile Gly Met Gly Gly Asp Lys Leu Val Pro Glu
50 55 60
Leu Thr Gly Leu Asp Ala Glu Ser Glu Arg Gly Gln Arg Leu Thr Asp
65 70 75 80
Gly Trp Ala Arg His Phe Arg Ala Leu Ile Pro Asp Leu Arg Pro Thr
85 90 95
Pro Gly Ala Arg Ala Leu Leu Glu Gly Leu Arg Ala Arg Gly Leu Arg
100 105 110
Val Ala Leu Ala Thr Ser Gly Glu Ala Glu Val Val Asp Ala Leu Leu
115 120 125
Gln Gln Ile Gly Leu Asp Asp Leu Glu Leu Glu Arg Val Ser Ser Ser
130 135 140
Asp Val Glu Asn Ser Lys Pro Asp Pro Asp Leu Val Gln Ala Gly Leu
145 150 155 160
Arg Thr Leu Gly Leu Pro Ala Glu Ala Thr Leu Met Val Gly Asp Thr
165 170 175
Pro Tyr Asp Ala Glu Ala Ala Arg Lys Ala Gly Val Pro Cys Val Leu
180 185 190
Leu Arg Cys Gly Gly His Pro Gly Val Glu Glu His Asp Pro Val Tyr
195 200 205
Asp His Pro Gln Ala Leu Leu Glu Ala Leu Asp Gln Ala Phe Pro Val
210 215 220
Glu
225
<210> 54
<211> 198
<212> PRT
<213> Deinococcus sp. RL
<400> 54
Met Ala His Ala Arg Ala Trp Ala Ala Ala Leu Arg Asp Glu Gly Phe
1 5 10 15
Glu Gln Ser Pro Glu Ala Ile Leu Pro Leu Ile Gly Met Gly Gly Asp
20 25 30
Lys Leu Val Pro Glu Leu Thr Gly Leu Asp Ala Glu Ser Glu Arg Gly
35 40 45
Gln Arg Leu Thr Asp Gly Trp Ala Arg His Phe Arg Ala Leu Ile Pro
50 55 60
Asp Leu Arg Pro Thr Pro Gly Ala Arg Ala Leu Leu Glu Gly Leu Arg
65 70 75 80
Ala Arg Gly Leu Arg Val Ala Leu Ala Thr Ser Gly Glu Ala Glu Val
85 90 95
Val Asp Ala Leu Leu Gln Gln Ile Gly Leu Asp Asp Leu Glu Leu Glu
100 105 110
Arg Val Ser Ser Ser Asp Val Glu Asn Ser Lys Pro Asp Pro Asp Leu
115 120 125
Val Gln Ala Gly Leu Arg Thr Leu Gly Leu Pro Ala Glu Ala Thr Leu
130 135 140
Met Val Gly Asp Thr Pro Tyr Asp Ala Glu Ala Ala Arg Lys Ala Gly
145 150 155 160
Val Pro Cys Val Leu Leu Arg Cys Gly Gly His Pro Gly Val Glu Glu
165 170 175
His Asp Pro Val Tyr Asp His Pro Gln Ala Leu Leu Glu Ala Leu Asp
180 185 190
Gln Ala Phe Pro Val Glu
195
<210> 55
<211> 223
<212> PRT
<213> Deinococcus sp. YIM 77859
<400> 55
Met Ile Ala Glu Glu Leu Leu Ser Pro Asp Arg Leu Arg Gly Val Leu
1 5 10 15
Leu Asp Val Asp Gly Thr Leu Val Asp Ser Asn Asp Ala His Ala Tyr
20 25 30
Ala Trp Leu Glu Ala Leu Arg Glu Glu Gly Phe Thr Arg Thr Phe Glu
35 40 45
Glu Val Arg Pro Leu Ile Gly Met Gly Gly Asp Gln Leu Val Pro Arg
50 55 60
Leu Thr Gly Glu Asp Gly Glu Ser Glu Arg Gly Lys Arg Leu Thr Arg
65 70 75 80
Gly Trp Leu Asn His Phe Lys Pro Arg Ile Pro Gln Leu Gln Ala Thr
85 90 95
Arg Gly Ala Arg Ala Leu Val Glu Gly Leu Gln Ala Arg Gly Leu Arg
100 105 110
Val Val Leu Ala Thr Ser Gly Glu Ala Asp Ile Val Asp Gly Leu Leu
115 120 125
Lys Arg Ala His Leu Glu Asp Leu Asn Leu Asp Arg Val Ser Ser Ser
130 135 140
Glu Val Glu Ser Ser Lys Pro Ala Pro Asp Leu Ile Gln Ala Gly Leu
145 150 155 160
Lys Lys Leu Gly Leu Pro Ala Glu Ala Ala Leu Met Val Gly Asp Thr
165 170 175
Pro Tyr Asp Ala Glu Ala Ala Arg Lys Ala Gly Val Pro Tyr Val Leu
180 185 190
Leu Arg Cys Gly Gly Asn Thr Gly Leu Glu Glu His Gly Pro Thr Leu
195 200 205
Asp Asp Pro Gln Ala Leu Leu Glu Ala Leu Glu Lys Ala Ser Ala
210 215 220
<210> 56
<211> 240
<212> PRT
<213> 粘硫还原球菌(Desulfurococcus mucosus)
<400> 56
Met Ala Val Lys Ile Val Phe Thr Asp Ile Asp Gly Thr Leu Thr Val
1 5 10 15
Asn Arg Glu Ser Tyr Arg Leu Ala Val Glu Ala Val Glu Ala Leu Arg
20 25 30
Met Leu Val Asp Lys Gly Val Ile Val Ser Leu Val Ser Ser Asn Ala
35 40 45
Leu Pro Val Val Val Ala Leu Ser Arg Tyr Ile Gly Leu Asn Gly Pro
50 55 60
Val Val Gly Glu Ser Gly Ala Leu Val Tyr His Asp Glu Trp Gly Leu
65 70 75 80
Val Glu Leu Ala Gly Glu Ser Ala Arg Gln Val Tyr Met Asp Leu Leu
85 90 95
Gly Asn Tyr Arg Glu Tyr Val Glu Asp Ser Trp Gln Asn Lys Phe Arg
100 105 110
Leu Tyr Glu Tyr Ala Leu Lys Leu Arg Arg Glu His Arg Gly Arg Ala
115 120 125
Arg Glu Val Val Asp Glu Leu Arg Arg Tyr Val Glu Ser Lys Tyr Lys
130 135 140
Gly Phe Thr Val Asp Tyr Ser Gly Tyr Ala Ile His Val His Ala Lys
145 150 155 160
Gly Val Gly Lys Gly Val Ala Val Asp Tyr Ile Leu Arg Arg Leu Gly
165 170 175
Leu Ser Gly Gly Glu Ala Leu Gly Ile Gly Asp Ser Tyr Met Asp Val
180 185 190
Asp Phe Ile Ala Arg Leu Gly Tyr Arg Ala Ala Val Gly Gly Ala Asp
195 200 205
Glu Glu Leu Val Arg Val Cys Asn Ile Val Ala Glu Ala Pro Ser Gly
210 215 220
Leu Gly Val Ala Glu Val Val His Arg Ile Leu Gly Glu Arg His Glu
225 230 235 240
<210> 57
<211> 230
<212> PRT
<213> 粘硫还原球菌(Desulfurococcus mucosus)
<400> 57
Met Asn Ser Leu Leu Arg Gly Ile Arg Leu Ile Leu Phe Asp Leu Asp
1 5 10 15
Gly Thr Ile Val Asp Ser Glu Asp Phe Ile Val Trp Ser Phe Val Glu
20 25 30
Ala Gly Arg Leu Thr Gly Ile Arg Val Asp Pro Leu Lys Val Arg Glu
35 40 45
Leu Ile Gly Phe Pro Leu Glu Ser Ile Leu Glu Ala Val Val Gly Thr
50 55 60
Gly Leu Ser Arg Glu Asp Ala Ala Arg Phe Ile Glu Val Arg Arg Lys
65 70 75 80
Leu Val Gln Glu Asn Trp Phe Lys His Val Arg Leu Phe Pro Asp Val
85 90 95
Ile Pro Val Leu Arg Gln Leu Ser Ser Thr Gly Tyr Met Leu Gly Val
100 105 110
Ala Ser Ser Ser Ile Arg Glu Arg Val Glu Leu Phe Leu Ser His Leu
115 120 125
Gly Val Ser Gly Tyr Phe Lys Val Val Ser Gly Leu Glu Pro Gly Val
130 135 140
Lys Gly Lys Pro Glu Pro Asp Val Ile Val Asn Ala Leu Lys Ala Ala
145 150 155 160
Gly Val Pro Arg Ser Glu Ala Leu Tyr Val Gly Asp Arg Met Val Asp
165 170 175
Cys Ile Ala Ala Arg Arg Ala Gly Val Lys Val Val Ile Val Asp Arg
180 185 190
Gly Ser Val Asn Arg Trp Lys Gln Glu Glu Cys Val Pro Asp Ala Trp
195 200 205
Ile Ser Ser Leu Leu Glu Leu Val Asp Asp Ala Ser Thr Pro Thr Arg
210 215 220
Glu Ala Thr Arg His Asp
225 230
<210> 58
<211> 224
<212> PRT
<213> 粘硫还原球菌(Desulfurococcus mucosus)
<400> 58
Met Arg Ile Arg Leu Val Val Met Asp Tyr Asp Leu Ser Met Val Tyr
1 5 10 15
Asn Leu Ser Asp Phe Tyr Glu Ala Tyr Ser Gly Ala Leu Lys Ser Tyr
20 25 30
Gly Gly Gly Phe Ile Ser Phe Asp Glu Phe Leu Asn Leu Leu Asn Glu
35 40 45
Asp Arg Leu Ser Glu Arg Ile Pro Gly Gly Val Arg Glu Glu Glu Phe
50 55 60
Trp Leu Leu Phe Arg Arg Ile Tyr Val Ser Arg His Pro Ile Pro Met
65 70 75 80
Asp Gly Val Val Asp Leu Leu Arg Leu Leu Lys Ser Leu Pro Val Lys
85 90 95
Ile Ala Val Val Ser Gly Arg Glu Thr Ser Gly Glu Tyr Ile Trp Arg
100 105 110
Asp Leu Arg Met Leu Gly Leu Asp Glu Tyr Val Asp Glu Val Tyr Thr
115 120 125
Val Ser Asp Leu Gln Arg Leu Asn Gly Leu Glu Glu Tyr Leu Phe Asp
130 135 140
Lys Ser Trp Ile Ile Ser Tyr Ile Leu Arg Lys His Gly Val Ala Pro
145 150 155 160
Cys Asn Ala Leu Cys Ile Gly Asp Phe Thr Thr Asp Leu Leu Ser Cys
165 170 175
Arg Lys Leu Gly Ile Pro Phe Ile Gly Val Asn Lys Asp Glu Tyr Arg
180 185 190
Gly Met Leu Leu Lys Lys Arg Gly Ala Ala Ile Val Val Lys Asn Leu
195 200 205
Phe Glu Val Val Gln Trp Ile Pro Glu Leu Glu Lys Asp Ser Thr Cys
210 215 220
<210> 59
<211> 265
<212> PRT
<213> 嗜热网球菌(Dictyoglomus turgidum)
<400> 59
Met Lys Arg Ile Leu Glu Val Ala Ile Lys Thr Ile Lys Glu Ser Gly
1 5 10 15
Asn Ile Leu Leu Asn Tyr Ile Gly Glu Glu Lys Glu Ile Glu Leu Lys
20 25 30
Gly Ile Ser Asn Leu Val Thr Gln Val Asp Lys Leu Ser Glu Arg His
35 40 45
Ile Leu Lys Ser Ile Glu Glu Asn Phe Pro Asp His Ser Ile Leu Thr
50 55 60
Glu Glu Thr Gly Phe Ile Asn Lys Asn Ser Glu Tyr Thr Trp Ile Val
65 70 75 80
Asp Pro Leu Asp Gly Thr Thr Asn Tyr Ala His Asn Phe Pro Phe Phe
85 90 95
Gly Ile Ser Ile Ala Leu Ile Lys Asn Lys Glu Ile Ile Leu Gly Leu
100 105 110
Ile Tyr Asp Pro Ile Arg Asp Glu Leu Phe Tyr Ala Ile Lys Asn Glu
115 120 125
Gly Ala Tyr Leu Asn Asp Arg Arg Ile Glu Val Ser Lys Thr Glu Ser
130 135 140
Leu Glu Asn Ser Leu Ile Ser Phe Ala Phe Pro Tyr Glu Leu Ser Leu
145 150 155 160
Glu Glu Lys Asn Phe Ile Pro Phe Ile Asn Phe Ser Ser Arg Thr His
165 170 175
Gly Ile Arg Arg Thr Gly Ser Ala Ala Ile Glu Ile Ala Tyr Val Gly
180 185 190
Cys Gly Arg Leu Asp Gly Phe Trp Ala Lys Lys Leu Lys Pro Trp Asp
195 200 205
Ile Ser Ala Gly Ile Leu Ile Val Glu Glu Ala Lys Gly Lys Val Thr
210 215 220
Asp Phe Ser Gly Asn Asn Ile Asp Ile His Thr Asp Asn Ile Leu Phe
225 230 235 240
Ser Asn Gly Lys Ile His Gln Glu Met Ile Lys Ile Leu Asn Leu Gly
245 250 255
Lys Ile Phe Ile Arg Asn Glu Lys Phe
260 265
<210> 60
<211> 249
<212> PRT
<213> 嗜热网球菌(Dictyoglomus turgidum)
<400> 60
Met Tyr Lys Leu Phe Ile Thr Asp Leu Asp Gly Ser Ile Met Asp Gly
1 5 10 15
Ser Glu Val Ile Ser Thr Lys Asn Leu Lys Ala Ile Lys Ile Leu Arg
20 25 30
Glu Asn Asn Ile Glu Ile Thr Ile Ala Thr Gly Arg Arg Trp Ser Ser
35 40 45
Ile Ser Lys Ile Val Glu Pro Leu Ser Leu Thr Leu Pro Val Ile Leu
50 55 60
Tyr Asn Gly Ala Gly Ile Tyr Asp Pro Leu Arg Lys Ser Phe Leu Tyr
65 70 75 80
Leu Gln His Leu Ser Lys Glu Glu Val Ile Gly Ala Leu Lys Val Ile
85 90 95
His Tyr Tyr Leu Ser Phe Val Lys Leu Gly Ile Tyr His Asp Asp Lys
100 105 110
Leu Tyr Glu Asp Ser Glu Ala Leu Glu Phe Leu Lys Asp Asn Lys Asn
115 120 125
Gly Ile Ile Lys Ile Phe Ile Glu Gly Glu Lys Glu Leu Leu Lys Glu
130 135 140
Leu Lys Asn Arg Leu Glu Lys Ile Tyr Leu Asn Val Val Phe Ser Ser
145 150 155 160
Tyr Lys Tyr Leu Glu Ile Leu Pro Lys Gly Ala Ser Lys Gly Lys Ala
165 170 175
Leu Lys Lys Leu Leu Lys Asn Leu Asp Ile Lys Leu Glu Glu Val Ile
180 185 190
Ala Leu Gly Asp Tyr Asp Asn Asp Glu Glu Met Leu Lys Leu Ser Gly
195 200 205
Leu Gly Ile Thr Leu Lys Asn Ala Ser Glu Arg Leu Lys Lys Ile Ala
210 215 220
Asp Tyr Val Ile Asp Ala Ser Pro Ser Glu Ser Val His Tyr Ile Ile
225 230 235 240
Gln Gln Val Leu Asn Phe Glu Arg Arg
245
<210> 61
<211> 213
<212> PRT
<213> 嗜热网球菌(Dictyoglomus turgidum)
<400> 61
Met Glu Leu Lys Gly Ile Ile Phe Asp Trp Asp Gly Thr Leu Val Asp
1 5 10 15
Ser Phe Pro Ala Cys Ile Lys Ala Thr Lys Glu Ile Phe Lys Lys Phe
20 25 30
Gly Phe Ile Ile Ser Glu Glu Glu Tyr Arg Glu Lys Phe Ser Pro Asn
35 40 45
Trp Tyr Asp Ile Tyr Arg Lys His Asn Ile Pro Glu Lys Tyr Trp Lys
50 55 60
Glu Ile Asp Leu Leu Trp Lys Asp Tyr Phe Asp Tyr Ser Leu Val Lys
65 70 75 80
Trp Arg Glu Gly Ala Val Glu Asn Leu Thr Phe Leu Lys Lys Leu Asn
85 90 95
Leu Lys Ile Gly Val Val Thr Ala Ser Thr Lys Glu Asp Ile Glu Arg
100 105 110
Glu Lys Pro Tyr Leu Asn Pro Glu Glu Tyr Ile Asp Gly Trp Ile Thr
115 120 125
Trp Glu Asp Ser Glu Lys Pro Lys Pro Asp Pro Leu Pro Leu Leu Lys
130 135 140
Ile Leu Lys Lys Leu Ser Leu Ser Pro Leu Glu Val Ile Tyr Val Gly
145 150 155 160
Asp Thr Lys Glu Asp Ile Glu Met Ser Lys Lys Leu Asn Ile Leu Thr
165 170 175
Ile Gly Val Leu Ser Pro Phe Thr Gln Lys Glu Lys Leu Val Ser Ser
180 185 190
Asn Pro Asn Phe Leu Phe Asn Asn Leu Phe Glu Leu Leu Lys Phe Trp
195 200 205
Lys Asp Leu Phe Leu
210
<210> 62
<211> 213
<212> PRT
<213> Effusibacillus pohliae
<400> 62
Met Lys Tyr Pro Tyr Ile Leu Phe Asp Leu Asp Gly Thr Leu Val Asp
1 5 10 15
Thr Asn Glu Leu Ile Leu Gln Ser Phe Glu His Thr Leu Glu His Tyr
20 25 30
Phe Pro Gly Arg Tyr Ser Arg Gln Asp Val Leu Pro Tyr Met Gly Glu
35 40 45
Pro Leu Val Lys Gln Phe Ala Arg Trp Ala Pro Pro Glu Gln Val Ser
50 55 60
Glu Leu Val Gln Ala Tyr Arg Lys Tyr Asn Val Glu Asn His Asp Arg
65 70 75 80
Leu Ala Thr Ile Phe Pro Asn Val Pro Glu Val Leu Ala Glu Leu Lys
85 90 95
Ala Gln Gly Cys Arg Met Ala Val Val Thr Asn Lys Met Arg Leu Thr
100 105 110
Ala Glu Arg Gly Leu Arg Leu Phe Gly Leu Asp Arg Tyr Leu Glu Thr
115 120 125
Val Val Thr Val Glu Asp Thr Asp Gln His Lys Pro His Pro Ala Pro
130 135 140
Leu Gln Ile Ala Ile Glu Arg Leu Gly Gly Glu Pro Ala Gly Thr Leu
145 150 155 160
Met Val Gly Asp Ser Pro Tyr Asp Ile Leu Ala Gly Gln Ala Ala Gly
165 170 175
Thr Ala Thr Cys Ala Val Ala Trp Ser Leu Arg Gly Cys Glu Gly Leu
180 185 190
Ala Ser Tyr Lys Pro Asp Tyr Leu Ile Asp Glu Met Arg Glu Leu Leu
195 200 205
Glu Ile Val Arg Gly
210
<210> 63
<211> 215
<212> PRT
<213> Fervidobacterium gondwanense
<400> 63
Met Arg Pro Lys Ala Cys Ile Phe Asp Leu Asp Gly Val Ile Val Asp
1 5 10 15
Thr Ala Lys Tyr His Tyr Leu Ala Trp Lys Arg Leu Ala Lys Glu Leu
20 25 30
Gly Phe Glu Phe Thr Glu Lys Asp Asn Glu Arg Leu Lys Gly Val Ser
35 40 45
Arg Met Glu Ser Leu Glu Ile Leu Leu Ser Val Gly Gly Ile Lys Ile
50 55 60
Glu Asp Gln Lys Leu Lys Glu Gln Leu Ser Asp Lys Lys Asn Asn Trp
65 70 75 80
Tyr Val Glu Tyr Ile Ser Gln Met Thr Lys Asp Glu Ile Leu Pro Gly
85 90 95
Val Glu Glu Phe Leu Arg Lys Leu Lys Asn Ala Gly Ile Lys Ile Ala
100 105 110
Ile Gly Ser Ala Ser Lys Asn Thr Met Thr Ile Leu Arg Arg Ile Glu
115 120 125
Leu Val Asp Leu Phe Asp Ser Ile Ile Asp Gly Thr Lys Ile Thr Lys
130 135 140
Ala Lys Pro Asp Pro Glu Val Phe Leu Lys Ala Ala Glu Glu Leu Asn
145 150 155 160
Val Asp Pro Lys Asp Cys Cys Val Phe Glu Asp Ala Val Ala Gly Ile
165 170 175
Glu Ala Ala Lys Arg Ala Gly Met Lys Val Ile Gly Val Gly Asp His
180 185 190
Glu Ile Leu Lys Asp Ala Asp Arg Val Ile Thr Ser Phe Val Gly His
195 200 205
Gly Ile Glu Leu Val Glu Phe
210 215
<210> 64
<211> 305
<212> PRT
<213> Fervidobacterium islandicum
<400> 64
Met Lys Glu Trp Arg Glu Glu Glu Leu Ser Asn Lys Ser Lys Cys Lys
1 5 10 15
Arg Thr Phe Val Phe Asp Leu Asp Gly Thr Val Leu Asn Ser Lys Asn
20 25 30
Glu Phe Pro Lys Glu Thr Lys Thr Leu Val Gln Asn Ile Val Gly Asn
35 40 45
Gly Asp Asn Val Val Phe Ala Thr Gly Arg Met His Ile Ser Ala Lys
50 55 60
Lys Leu Leu Asp Asn Val Phe Gly Glu Asp Ile Phe Pro Ile Ile Ser
65 70 75 80
Tyr Asn Gly Ala Val Ile Tyr Val Pro Gly Glu Gly Phe Ile Tyr Glu
85 90 95
Lys Thr Leu Asp Ile Glu Thr Ala Tyr Lys Val Ile Asp Phe Leu Arg
100 105 110
Glu Lys Asn Ile His Ile Gln Thr Tyr Val Asp Asp Asn Leu Tyr Ser
115 120 125
Glu Lys Asp Asp Asp Glu Ile Arg Leu Tyr Ala Lys His Ala Asp Val
130 135 140
Pro Tyr Tyr Val Val Glu Asp Leu Lys Ala Leu Glu His Pro Pro Ile
145 150 155 160
Lys Met Leu Ala Ile Ala Glu Glu Ala Ile Leu Asp Ser Leu Ile Glu
165 170 175
Pro Leu Lys Glu Ile Val Asp Gly Lys Ala Asn Val Phe Lys Ser Phe
180 185 190
Pro Ile Phe Leu Asp Ile Val Pro Ala Asp Ala Asn Lys Gly Ile Ala
195 200 205
Leu Lys Phe Leu Ala Asp Tyr Leu Asn Phe Asp Leu Gly Ser Thr Ile
210 215 220
Val Phe Gly Asp Asn Glu Asn Asp Ile Tyr Met Phe Lys Val Ala Ser
225 230 235 240
Lys Arg Ile Ala Val Ser Asn Ala Val Trp Arg Leu Lys Glu Val Ala
245 250 255
Asp Phe Val Ser Lys Ser Asn Glu Glu Asn Gly Val Leu His Ala Phe
260 265 270
Val His Leu Phe Pro Glu Tyr Val Arg Gly Ile Asp Phe Ser Ser Gly
275 280 285
His Thr Asn Ser Asn Ile Val Asn Thr Thr Ser Asp Asn Lys Ala Ala
290 295 300
Glu
305
<210> 65
<211> 278
<212> PRT
<213> Fervidobacterium islandicum
<400> 65
Met Val Lys Leu Ile Val Thr Asp Leu Asp Gly Thr Leu Leu Asn Asp
1 5 10 15
Glu Lys His Ile Pro Lys Glu Asn Ile Glu Val Leu Arg Ala Ala Met
20 25 30
Glu Lys Gly Ile His Val Ser Val Ala Thr Gly Arg Asn Phe Tyr Ser
35 40 45
Ala Lys Pro Tyr Val Glu Glu Leu Gly Leu Asp Val Pro Val Ile Phe
50 55 60
Gln Asn Gly Ala Phe Ile Tyr Gln Trp Met Glu Asn Lys Ile Ile Tyr
65 70 75 80
Lys Ser Ala Leu Gly Ser Glu Ile Ala Thr Lys Val Ile Glu Thr Ala
85 90 95
Arg Arg His Lys Leu Phe Tyr Ile Leu Tyr Arg Asp Phe Leu Glu Glu
100 105 110
Lys Asp Met Tyr Ile Asp Met Pro Tyr Thr Gly Ser Phe Ser Leu Tyr
115 120 125
Leu Gly Gln Asn Gln Trp Arg Leu Asn Ile Val Asp Asp Val Leu Lys
130 135 140
Tyr Ile Lys Thr Lys Asp Ala Val Ala Glu Val Ala Leu Val Gly Glu
145 150 155 160
Glu Leu Val Ile Arg Gln Ala Ile Asn Glu Ala Leu Asp Gly Leu Glu
165 170 175
Ser Gln Thr Ser Val Val Lys Asn Thr Thr Ile Gly Asn Glu Thr Phe
180 185 190
Tyr Glu Ile Phe Gly Pro Asn Ser Ser Lys Glu Leu Ser Ile Lys His
195 200 205
Leu Phe Glu Tyr Phe Lys Val Ala Pro Glu Glu Thr Met Tyr Leu Gly
210 215 220
Asp Ser Phe Asn Asp Ile Gly Leu Leu Lys Met Val Gly Tyr Pro Ile
225 230 235 240
Val Met Glu Asn Gly His Asp Glu Val Lys Gln Tyr Ala Arg Tyr Ile
245 250 255
Thr Lys Ser Asn Asn Glu Ala Gly Val Ala Tyr Ala Val Lys Lys Trp
260 265 270
Ala Leu Gly Ile Asp Asp
275
<210> 66
<211> 270
<212> PRT
<213> Fervidobacterium nodosum
<400> 66
Met Val Arg Lys Thr Lys Thr Phe Val Phe Asp Leu Asp Gly Thr Val
1 5 10 15
Leu Thr Pro Lys Asn Glu Phe Pro Leu Glu Thr Lys Glu Leu Ile Met
20 25 30
Asn Ile Leu Lys Lys Gly Asp Asn Val Val Phe Ala Thr Gly Arg Met
35 40 45
His Ile Ser Ala Lys Lys Leu Leu Asp Arg Val Phe Gly Glu Asp Val
50 55 60
Phe Pro Ile Ile Ser Tyr Asn Gly Ala Val Val Tyr Val Pro Asp Lys
65 70 75 80
Gly Phe Ile Phe Glu Lys Thr Leu Glu Leu Ser Thr Ala His Lys Val
85 90 95
Ile Asp Phe Phe Arg Arg Asn Asn Ile His Val Gln Thr Tyr Val Glu
100 105 110
Asp Ile Leu Tyr Thr Glu Lys Asp Asn Glu Glu Ile Lys Leu Tyr Ser
115 120 125
Lys His Ala Glu Val Pro Tyr Thr Val Val Asp Asp Leu Lys Ala Ile
130 135 140
His Gln Pro Pro Ile Lys Ile Leu Gly Ile Gly Asp Gln Glu Leu Leu
145 150 155 160
Asp Arg Leu Ile Pro Asp Leu Lys Asn Val Val Gly Asn Ser Ala Thr
165 170 175
Val Phe Lys Ser Phe Ser Ile Phe Leu Asp Val Val Pro Ala Asp Ala
180 185 190
Asn Lys Gly Ile Ala Leu Arg Phe Leu Ala Asp Tyr Leu Lys Phe Asp
195 200 205
Leu Ser Glu Thr Ile Val Phe Gly Asp Asn Glu Asn Asp Ile Phe Met
210 215 220
Phe Glu Val Ala Gly Arg Lys Ile Ala Val Ser Asn Ala Val Glu Lys
225 230 235 240
Leu Lys Glu Val Ala Asp Phe Val Ser Lys Ser Asn Glu Glu Asn Gly
245 250 255
Val Tyr Phe Ala Phe Thr Gln Leu Phe Pro Glu Tyr Ile Val
260 265 270
<210> 67
<211> 276
<212> PRT
<213> Fervidobacterium nodosum
<400> 67
Met Val Lys Leu Ile Val Thr Asp Leu Asp Gly Thr Leu Leu Asn Asp
1 5 10 15
Asp Lys His Ile Pro Asp Asp Asn Ile Ile Ala Leu Arg Glu Ala Met
20 25 30
Glu Lys Gly Val His Val Ser Ile Ala Thr Gly Arg Asn Phe Gly Ser
35 40 45
Ala Lys Arg Tyr Ile Lys Glu Leu Gly Leu Asp Val Pro Val Ile Phe
50 55 60
Gln Asn Gly Ala Phe Ile Tyr Gln Trp Met Glu Asp Lys Val Ile Tyr
65 70 75 80
Lys Ser Asp Leu Lys Ser Glu Ile Ala Lys Leu Ile Val Glu Lys Ala
85 90 95
Arg Glu Lys Gly Leu Phe Tyr Val Val Tyr Ile Asp Phe Leu Glu Glu
100 105 110
Lys Asp Met Tyr Ile Asp Ala Asn Tyr Ser Gly Glu Phe Leu Ser Tyr
115 120 125
Leu Lys Gln Asn Glu Trp Arg Ile Asn Tyr Val Ser Asp Val Val Asn
130 135 140
Tyr Ile Ser Asn Arg Asp Ser Ile Ala Glu Val Ala Leu Val Gly Asp
145 150 155 160
Glu Glu Lys Ile Lys Asn Ile Val Glu Asp Asp Leu Phe Ile Phe Gly
165 170 175
Glu Ser Val Ser Val Val Lys Asn Asn Arg Ile Asn Ser Glu Val Phe
180 185 190
Tyr Glu Phe Phe Gly Pro Asn Ser Ser Lys Asp Ile Ser Phe Asn Tyr
195 200 205
Leu Leu Lys Tyr Phe Asn Val Lys Pro Glu Glu Thr Met Tyr Leu Gly
210 215 220
Asp Asn Tyr Asn Asp Ile Gly Met Leu Lys Ile Val Gly Tyr Pro Val
225 230 235 240
Val Met Glu Asn Ala Pro Asp Glu Val Lys Lys Tyr Ala Lys Tyr Val
245 250 255
Ser Lys Ser Asn Asn Glu Ala Gly Val Ala Tyr Ala Val Arg Lys Leu
260 265 270
Val Leu Gly Tyr
275
<210> 68
<211> 281
<212> PRT
<213> Fervidobacterium pennivorans
<400> 68
Met Ser Asn Ile Arg Lys Leu Lys Asn Ala Lys Thr Phe Val Phe Asp
1 5 10 15
Leu Asp Gly Thr Val Leu Asn Ser Lys Asn Glu Phe Pro Gln Glu Thr
20 25 30
Lys Gln Leu Ile Leu Asn Ile Leu Glu Asn Gly Asp Asn Val Ile Phe
35 40 45
Ala Thr Gly Arg Met His Ile Ser Ala Lys Lys Leu Leu Glu Asn Val
50 55 60
Phe Gly Glu Asp Ile Phe Pro Ile Ile Ser Tyr Asn Gly Ala Val Val
65 70 75 80
Tyr Val Pro Asn Val Gly Phe Ile Tyr Glu Lys Thr Leu Asp Met Glu
85 90 95
Thr Val His Lys Val Ile Gly Phe Leu Arg Gln Arg Asn Ile His Val
100 105 110
Gln Thr Tyr Val Asp Asp Lys Leu Tyr Ser Glu Lys Asp Asn Glu Glu
115 120 125
Ile Lys Leu Tyr Ala Lys His Ala Asp Val Pro Tyr Tyr Val Val Asn
130 135 140
Asp Leu Lys Ala Ile Glu His Pro Pro Ile Lys Met Leu Ala Ile Ala
145 150 155 160
Glu Gln Gly Ile Leu Asp Asn Leu Ile Glu Pro Leu Lys Glu Ile Val
165 170 175
Asp Gly Arg Ala Asn Val Phe Lys Ser Phe Pro Ile Phe Leu Asp Ile
180 185 190
Val Pro Ala Asp Ala Asn Lys Gly Asn Ala Leu Lys Phe Leu Ala Asp
195 200 205
Tyr Leu Gly Phe Asp Leu Lys Asn Thr Val Val Phe Gly Asp Asn Glu
210 215 220
Asn Asp Ile Tyr Met Phe Glu Val Ala Gly Thr Lys Val Ala Val Glu
225 230 235 240
Asn Ala Val Asp Lys Leu Lys Glu Val Ala Asp Phe Val Ser Lys Ser
245 250 255
Asn Glu Glu Asn Gly Val Leu Tyr Ser Phe Val Arg Leu Phe Pro Glu
260 265 270
Tyr Leu Arg Gly Ile Glu Ser Ser Asn
275 280
<210> 69
<211> 278
<212> PRT
<213> Fervidobacterium pennivorans
<400> 69
Met Val Lys Leu Ile Val Thr Asp Leu Asp Gly Thr Leu Leu Asn Asp
1 5 10 15
Glu Lys His Ile Pro Arg Glu Asn Ile Glu Ala Leu Lys Ala Ala Met
20 25 30
Asp Lys Gly Ile His Val Ser Val Ala Thr Gly Arg Asn Phe Tyr Ser
35 40 45
Ala Lys Pro Tyr Val Glu Lys Leu Gly Leu Asp Val Pro Val Ile Phe
50 55 60
Gln Asn Gly Ala Phe Ile Tyr Gln Trp Met Glu Asp Lys Val Ile Tyr
65 70 75 80
Lys Ser Glu Leu Lys Thr Glu Ile Ala Glu Arg Val Ile Glu Thr Ala
85 90 95
Arg Lys Tyr Lys Ile Phe Tyr Ile Leu Tyr Arg Asp Phe Leu Glu Glu
100 105 110
Lys Asp Met Tyr Ile Asp Gln Pro Tyr Ser Gly Asn Phe Ser Leu Tyr
115 120 125
Leu Asn Gln Asn Arg Trp Arg Leu Asn Ile Val Asp Asp Val Leu Lys
130 135 140
Tyr Leu Lys Asn Lys Leu Thr Val Ala Glu Val Ala Leu Val Gly Asn
145 150 155 160
Glu Glu Arg Ile Met Leu Ala Leu Asn Glu Ser Leu Gly Glu Val Arg
165 170 175
Asn Ala Thr Ser Ile Val Lys Asn Asn Thr Ile Asn Glu Glu Thr Phe
180 185 190
Tyr Glu Val Phe Gly Pro Asn Ala Ser Lys Glu Leu Ser Ile Lys Tyr
195 200 205
Leu Phe Glu Tyr Phe Asn Val Ala Pro Glu Glu Thr Met Tyr Leu Gly
210 215 220
Asp Ser Phe Asn Asp Ile Gly Leu Leu Lys Met Val Gly Tyr Pro Val
225 230 235 240
Val Met Glu Asn Gly His Ser Glu Val Lys Gln Tyr Ala Arg Tyr Ile
245 250 255
Thr Lys Ser Asn Asn Glu Ala Gly Val Ala Tyr Ala Val Arg Lys Trp
260 265 270
Ala Leu Gly Ile Asp Glu
275
<210> 70
<211> 215
<212> PRT
<213> Fervidobacterium pennivorans
<400> 70
Met Lys Pro Lys Ala Cys Ile Phe Asp Leu Asp Gly Val Ile Val Asp
1 5 10 15
Thr Ala Lys Tyr His Tyr Leu Ala Trp Lys Arg Leu Ala Lys Glu Leu
20 25 30
Gly Phe Glu Phe Thr Glu Lys Asp Asn Glu Arg Leu Lys Gly Val Ser
35 40 45
Arg Met Glu Ser Leu Glu Ile Leu Leu Ser Val Gly Gly Ile Lys Ile
50 55 60
Asp Asp Glu Asn Val Lys Leu Gln Leu Ala Glu Lys Lys Asn Lys Trp
65 70 75 80
Tyr Val Glu Tyr Ile Asn Gln Met Thr Lys Asp Glu Ile Leu Pro Gly
85 90 95
Val Ile Glu Phe Leu Glu Leu Leu Lys Ser Ala Gly Ile Lys Val Ala
100 105 110
Ile Gly Ser Ala Ser Lys Asn Thr Ile Thr Ile Leu Glu Arg Ile Gly
115 120 125
Leu Lys Asp Phe Phe Asp Val Ile Ile Asp Gly Thr Lys Ile Ser Lys
130 135 140
Ala Lys Pro Asp Pro Glu Val Phe Leu Lys Ala Ala Glu Glu Leu Ser
145 150 155 160
Val Arg Pro Glu Glu Cys Cys Val Phe Glu Asp Ala Val Ala Gly Ile
165 170 175
Gln Ala Ala Lys Ser Ala Gly Met Lys Val Ile Gly Val Gly Asp Pro
180 185 190
Thr Ile Leu Lys Asp Ala Asp Lys Val Ile Gln Ser Phe Gln Gly Gln
195 200 205
Thr Leu Gly Leu Ile Glu Phe
210 215
<210> 71
<211> 222
<212> PRT
<213> Geobacillus sp.
<400> 71
Met Thr Ile Arg Thr Ile Leu Phe Asp Leu Asp Gly Thr Leu Ile Asp
1 5 10 15
Thr Asn Glu Leu Ile Ile Gln Ser Phe Leu His Thr Leu Glu Lys Tyr
20 25 30
Tyr Pro Gly Arg Tyr Gly Arg Glu Asp Val Leu Pro Phe Ile Gly Pro
35 40 45
Ser Leu Tyr Glu Thr Phe Ser Ser Leu Asp Pro Glu Arg Ala Glu Glu
50 55 60
Met Val Lys Thr Tyr Arg Thr Phe Asn His Ala Arg His Asp Glu Leu
65 70 75 80
Ile Arg Glu Phe Asp Thr Val Tyr Glu Thr Ile Glu Thr Leu His Arg
85 90 95
His Gly Phe Arg Leu Gly Val Val Thr Thr Lys Met His Asp Thr Ala
100 105 110
Leu Met Gly Leu Arg Lys Thr Arg Leu Glu Pro Phe Phe Ser Cys Val
115 120 125
Ile Gly Leu Asp Asp Val Ser Arg Pro Lys Pro Asp Pro Glu Pro Ile
130 135 140
His Lys Ala Leu Glu Ala Leu Gln Ser Lys Ser Asp Glu Ala Leu Met
145 150 155 160
Val Gly Asp Asn Tyr His Asp Ile Leu Ala Gly Lys Asn Ala Gly Val
165 170 175
Lys Thr Ala Gly Val Ala Trp Ala Ile Lys Gly Arg Gly Tyr Leu Glu
180 185 190
Gln Tyr Glu Pro Asp Tyr Met Leu Glu Lys Met Ser Asp Leu Leu Ala
195 200 205
Ile Val Gly Ile Asn Glu Arg Lys Glu Glu Thr Ser Ser Gln
210 215 220
<210> 72
<211> 264
<212> PRT
<213> 嗜热脂肪地芽孢杆菌(Geobacillus stearothermophilus)
<400> 72
Met Ala Glu Lys Trp Glu Glu Ile Asp Arg Tyr Ala Arg Gln Trp Ile
1 5 10 15
Asp Glu Ala Gly Lys Arg Ile Arg Ala Ser Phe Ala Lys Gln Leu Thr
20 25 30
Val Glu Ala Lys Glu Asn Pro Asn Asp Leu Val Thr Asn Val Asp Arg
35 40 45
Ala Ile Glu Gln Phe Phe Ala Glu His Ile Arg Arg Gln Phe Pro Ser
50 55 60
His Arg Leu Leu Gly Glu Glu Gly Phe Gly Asp Arg Ile Asp Ala Leu
65 70 75 80
Asp Gly Val Val Trp Val Ile Asp Pro Ile Asp Gly Thr Met Asn Phe
85 90 95
Val His Gln Arg Arg His Phe Ala Val Ser Ile Gly Ile Phe Glu Asp
100 105 110
Gly Ile Gly Gln Leu Gly Tyr Val Tyr Asp Val Val Phe Asp Glu Leu
115 120 125
Tyr Ala Ala Gln Lys Gly Arg Gly Val Phe Leu Asn Gly Glu Pro Leu
130 135 140
Gly Leu Leu Gln Pro Ala Pro Val Ala Glu Ser Ile Ile Ala Ile Asn
145 150 155 160
Gly Thr Trp Leu Met Glu Asn Lys Arg Leu Asp His Arg Pro Leu Met
165 170 175
Arg Leu Ala Lys Glu Ala Arg Gly Thr Arg Ser Tyr Gly Ser Ala Ala
180 185 190
Leu Glu Leu Ala Tyr Val Ala Ala Gly Arg Leu Asp Ala Tyr Ile Ser
195 200 205
Pro Arg Leu Ser Pro Trp Asp Phe Ala Gly Gly Met Ile Leu Ile Glu
210 215 220
Glu Ala Gly Gly Met Val Thr Thr Leu Asp Gly Lys Pro Leu Asp Leu
225 230 235 240
Leu Gly Arg Asn Ser Val Leu Ala Ala Lys Pro Gly Val His Glu Glu
245 250 255
Ile Leu Arg Arg Tyr Leu His Asp
260
<210> 73
<211> 283
<212> PRT
<213> 嗜热脂肪地芽孢杆菌(Geobacillus stearothermophilus)
<400> 73
Met Leu Ile Ala Leu Asp Met Asp Gly Thr Leu Leu Asn Gly Glu Gly
1 5 10 15
Lys Ile Ser Glu Arg Asn Arg Lys Ala Met Met Ala Ala Gln Lys Glu
20 25 30
Gly His Ile Val Ala Val Ile Thr Gly Arg Ala Arg Lys Asp Ala Leu
35 40 45
Ala Pro Leu Arg Glu Ala Gly Leu Val Cys Pro Ile Ala Ser Leu Asn
50 55 60
Gly Ala Ile Val Thr Leu Glu Asp Gly Thr Val Ile Gly Glu Ala Pro
65 70 75 80
Leu Ala Arg Thr Ala Val Arg Pro Thr Leu Glu Trp Val Arg Lys Gln
85 90 95
Ser Asp Leu Tyr Cys Glu Thr Tyr Thr Gly Asp Ala Val Tyr Val Gly
100 105 110
Leu His Asn Arg Ala Gln Trp Glu Ser Leu Ala Ser Glu Met Ala Asp
115 120 125
Ala Ala Pro Asp Val Lys Trp Leu Val Asn Lys Gln Phe Gln Gln Ala
130 135 140
Arg Val Thr Tyr Val Asp Asp Ile Arg Thr Val Trp Asp Asp Pro Gln
145 150 155 160
Leu Thr Leu Tyr Lys Leu Leu Ile Phe Ala Leu Asp Arg Glu Arg Leu
165 170 175
Arg Asp Ala Ala Ser Arg Phe Ala Ala Leu Pro Ser Val Thr Val Thr
180 185 190
Ser Ser His Pro His Asn Ile Glu Met Asn Asn Glu Arg Ala Thr Lys
195 200 205
Gly Glu Ala Leu Lys Gln Leu Ala Ala His Tyr Gly Ile Asp Leu Cys
210 215 220
Asp Thr Val Ala Phe Gly Asp Ser His Asn Asp Leu Ser Met Phe Glu
225 230 235 240
Val Ala Gly Cys Arg Val Ala Met Ala Asn Ala Ala Pro Glu Leu Lys
245 250 255
Ala Lys Ala Asp Ile Val Thr Cys Ser His Glu Glu Asp Gly Val Ala
260 265 270
Ala Gly Leu Glu Arg Val Leu Ala Lys Arg Val
275 280
<210> 74
<211> 268
<212> PRT
<213> 嗜热脂肪地芽孢杆菌(Geobacillus stearothermophilus)
<400> 74
Met Lys Pro Tyr Leu Ile Ala Leu Asp Leu Asp Gly Thr Leu Leu Lys
1 5 10 15
Glu Asp Lys Thr Ile Ser Pro Phe Thr Lys Asp Val Ile Arg Arg Ala
20 25 30
Ile Asp Thr Gly His Phe Val Val Ile Ala Thr Gly Arg Pro Tyr Arg
35 40 45
Ala Ser Ser Met Tyr Tyr Glu Glu Leu Gly Leu Ala Thr Pro Ile Val
50 55 60
Asn Phe Asn Gly Ala Phe Val His His Pro Arg Gln Pro Ser Trp Gly
65 70 75 80
Met His His Tyr Pro Leu Pro Leu Ala Val Val Lys Asp Ile Val Glu
85 90 95
Ile Ser Glu Ser Tyr Gly Ile Lys Asn Met Met Ala Glu Val Leu Asp
100 105 110
Asn Val Tyr Phe His Gln His Asp Glu Val Leu Leu Asp Ile Val Arg
115 120 125
Leu Gly Asn Pro Thr Val Glu Ile Gly Asp Leu Arg Arg Ser Leu Gly
130 135 140
Lys Asp Pro Thr Ser Val Leu Val Tyr Thr Asp Asp Asp His Ile Glu
145 150 155 160
Arg Ile Gln Ser His Leu Ala Asn Val Tyr Ala Asn Val Ile His Gln
165 170 175
Arg Arg Trp Ser Glu Pro Trp His Val Ile Glu Ile Ile Arg His Gly
180 185 190
Val His Lys Ala Ala Gly Leu Lys Gln Val Ala Asp Tyr Phe Gly Ile
195 200 205
Pro Arg Glu Arg Val Ile Ala Phe Gly Asp Glu Asp Asn Asp Leu Glu
210 215 220
Met Ile Asp Trp Ala Gly Leu Gly Val Ala Met Gly Asn Ala Ile Glu
225 230 235 240
Pro Leu Lys Thr Ile Ala Asp Asp Val Ala Lys Thr Asn Glu Glu Asp
245 250 255
Gly Val Gly Val Tyr Leu Gln Asp Leu Leu Arg Leu
260 265
<210> 75
<211> 287
<212> PRT
<213> 嗜热脂肪地芽孢杆菌(Geobacillus stearothermophilus)
<400> 75
Met Tyr Lys Leu Leu Ala Leu Asn Ile Asp Gly Thr Ile Leu Lys Asn
1 5 10 15
Asn Gly Arg Leu Pro Arg Glu Thr Lys Glu Ala Val Asp Tyr Val Lys
20 25 30
Lys Lys Gly Val Tyr Val Thr Leu Ile Thr Ser Arg Asn Leu Leu Ser
35 40 45
Ala Arg Lys Val Ala Lys Ala Leu Arg Leu Asp Gly Met Leu Ile Ala
50 55 60
Phe Gln Gly Ala Met Ile Ala Arg Thr Leu Asp Asp Arg Leu Phe Asp
65 70 75 80
Ala Val Ile Pro Glu Glu Arg Thr Phe Asn Ile Val Gln Ile Leu Glu
85 90 95
Asn Phe Asn Cys Asn Ile Arg Leu Met His Glu Arg Tyr Ser Leu Gly
100 105 110
Asn Arg Lys Lys Val Lys Lys Asn Leu Val Val Gln Thr Val Leu Ser
115 120 125
Ser Ser Asp Pro Phe Phe Tyr Pro Thr Gln Phe Val Asp Ser Leu Gly
130 135 140
Asp Val Leu Met Asp Glu Pro Ile Ala Val Pro Lys Ile Asp Val Tyr
145 150 155 160
Phe Ala Thr Asp Glu Glu Arg Asp Ala Ala Ala Ala Leu Leu Thr Lys
165 170 175
Ser Ile Pro Ser Ile Asp Met Ile Met Gln Pro Asn Gly Lys Met Glu
180 185 190
Ile Val Pro Gln Gly Val Ser Lys Leu Ala Gly Leu Arg Arg Leu Val
195 200 205
Gln His Ile Gly Val Ser Leu Lys Glu Thr Val Met Ile Gly Asp Gly
210 215 220
Leu Asp Asp Leu Pro Ala Ile Glu Ala Ala Gly Leu Gly Val Ala Met
225 230 235 240
Gly Asn Ala Pro Leu Glu Val Lys Arg Ala Ala Asp Trp Val Thr Arg
245 250 255
Ser Asn Glu Gln Leu Gly Val Ala Tyr Met Val Lys Glu His Phe Arg
260 265 270
Lys Gln Gln Arg Ile Glu Phe Leu Gln Lys Leu Lys Thr Lys Gln
275 280 285
<210> 76
<211> 258
<212> PRT
<213> 嗜热脂肪地芽孢杆菌(Geobacillus stearothermophilus)
<400> 76
Met Gly Arg Lys Ile Val Phe Phe Asp Ile Asp Gly Thr Leu Leu Asp
1 5 10 15
Glu Gln Lys Gln Leu Pro Leu Ser Thr Ile Glu Ala Val Arg Arg Leu
20 25 30
Lys Gln Ser Gly Val Tyr Val Ala Ile Ala Thr Gly Arg Ala Pro Phe
35 40 45
Met Phe Glu His Val Arg Lys Gln Leu Gly Ile Asp Ser Phe Val Ser
50 55 60
Phe Asn Gly Gln Tyr Val Val Phe Glu Gly Asn Val Leu Tyr Lys Gln
65 70 75 80
Pro Leu Arg Arg Glu Lys Val Arg Ala Leu Thr Glu Glu Ala His Lys
85 90 95
Asn Gly His Pro Leu Val Phe Met Asp Ala Glu Lys Met Arg Ala Ser
100 105 110
Ile Ser Asp His Pro His Ile His Val Ser Met Ala Ser Leu Lys Phe
115 120 125
Ala His Pro Pro Val Asp Pro Leu Tyr Tyr Glu Asn Lys Asp Ile Tyr
130 135 140
Gln Ala Leu Leu Phe Cys Arg Ala Glu Glu Glu Glu Pro Tyr Val Arg
145 150 155 160
Asn Tyr Pro Glu Phe Arg Phe Val Arg Trp His Asp Val Ser Thr Asp
165 170 175
Val Leu Pro Ala Gly Gly Ser Lys Ala Glu Gly Ile Arg Met Met Ile
180 185 190
Glu Lys Leu Gly Ile Asp Lys Lys Asp Val Tyr Ala Phe Gly Asp Gly
195 200 205
Leu Asn Asp Ile Glu Met Leu Ser Phe Val Gly Thr Gly Val Ala Met
210 215 220
Gly Asn Ala His Glu Glu Val Lys Arg Val Ala Asp Phe Val Thr Lys
225 230 235 240
Pro Val Asp Lys Glu Gly Ile Trp Tyr Gly Leu Lys Gln Leu Gln Leu
245 250 255
Ile Arg
<210> 77
<211> 237
<212> PRT
<213> Halococcus salifodinae
<400> 77
Met Asn Arg Ala Asp Ala Ala Pro Glu Ala Arg Pro Pro Leu Ala Val
1 5 10 15
Asp Ile Asp Gly Thr Leu Thr Asp Arg Asn Arg Val Val Asp Pro Arg
20 25 30
Val Phe Asp Ala Leu Arg Glu Trp Pro Ala Pro Val Val Leu Ala Thr
35 40 45
Gly Lys Ala Leu Pro Tyr Pro Val Ala Leu Cys Glu Phe Ala Gly Leu
50 55 60
Ala Thr Leu Val Val Ala Glu Asn Gly Gly Val Val Cys Leu Asp Thr
65 70 75 80
Glu Thr Arg Asp Asp Ile Val Val Asp Gly Asp Arg Thr Ala Ala Asp
85 90 95
Arg Val Ala Ala Ala Tyr Arg Asp Ala Gly His Asp Leu Gly Trp Gly
100 105 110
Ser Leu Asp Leu Val Asn Arg Trp Arg Glu Thr Glu Ile Ala Val Ser
115 120 125
Arg Asp Ala Pro Leu Asp Pro Leu Arg Glu Ile Ala Val Glu Tyr Gly
130 135 140
Leu Asp Val Val Asp Thr Gly Phe Ala Tyr His Val Val Ser Pro Asp
145 150 155 160
Val Asp Lys Gly Arg Gly Leu Ser Ile Val Ala Asp Arg Leu Gly Arg
165 170 175
Asp Pro Glu Glu Phe Val Ala Ile Gly Asp Ser Ala Asn Asp Val Ala
180 185 190
Thr Phe Glu Val Val Gly Arg Ser Phe Ala Val Ala Asn Ala Asp Glu
195 200 205
Ala Ala Thr Gln Ala Ala Asp Leu Val Thr Asp Ala Ala Tyr Ala Asp
210 215 220
Gly Phe Ile Glu Ala Leu Ser Thr Val Arg Ser Ser Ser
225 230 235
<210> 78
<211> 237
<212> PRT
<213> Halococcus salifodinae
<400> 78
Met Asn Arg Ala Asp Ala Ala Pro Glu Ala Arg Pro Pro Leu Ala Val
1 5 10 15
Asp Ile Asp Gly Thr Leu Thr Asp Arg Asn Arg Val Val Asp Pro Arg
20 25 30
Val Phe Asp Ala Leu Arg Glu Trp Pro Ala Pro Val Val Leu Ala Thr
35 40 45
Gly Lys Ala Leu Pro Tyr Pro Val Ala Leu Cys Glu Phe Ala Gly Leu
50 55 60
Ala Thr Leu Val Val Ala Glu Asn Gly Gly Val Val Cys Leu Asp Thr
65 70 75 80
Glu Thr Arg Asp Asp Ile Val Val Asp Gly Asp Arg Thr Ala Ala Asp
85 90 95
Arg Val Ala Ala Ala Tyr Arg Asp Ala Gly His Asp Leu Gly Trp Gly
100 105 110
Ser Leu Asp Leu Val Asn Arg Trp Arg Glu Thr Glu Ile Ala Val Ser
115 120 125
Arg Asp Ala Pro Leu Asp Pro Leu Arg Glu Ile Ala Val Glu Tyr Gly
130 135 140
Leu Asp Val Val Asp Thr Gly Phe Ala Tyr His Val Val Ser Pro Asp
145 150 155 160
Val Asp Lys Gly Arg Gly Leu Ser Ile Val Ala Asp Arg Leu Gly Arg
165 170 175
Asp Pro Glu Glu Phe Val Ala Ile Gly Asp Ser Ala Asn Asp Val Ala
180 185 190
Thr Phe Glu Val Val Gly Arg Ser Phe Ala Val Ala Asn Ala Asp Glu
195 200 205
Ala Ala Thr Gln Ala Ala Asp Leu Val Thr Asp Ala Ala Tyr Ala Asp
210 215 220
Gly Phe Ile Glu Ala Leu Ser Thr Val Arg Ser Ser Ser
225 230 235
<210> 79
<211> 183
<212> PRT
<213> Halococcus salifodinae
<400> 79
Met Thr Val Thr Ile Pro Asp Asp Thr Glu Ala Val Val Tyr Asp Leu
1 5 10 15
Asp Gly Thr Leu Val Arg Leu Ala Val Asp Trp Asp Ala Ala Ala Thr
20 25 30
Asp Ala Ala Ala Ala Leu Asp Gly Cys Gly Val Asp Thr Asp Gly Met
35 40 45
Asp Leu Trp Arg Met Leu Glu Arg Ala Asp Glu Leu Gly His Arg Asp
50 55 60
Ala Val Glu Glu Ala Ile Ala Gly His Glu Arg Glu Gly Ala Arg Arg
65 70 75 80
Ser Glu Arg Leu Ala Phe Ala Asp Glu Leu Val Thr Asp Arg Pro Val
85 90 95
Gly Val Cys Ser Leu Asn Cys Glu Asp Ala Cys Arg Ile Ala Leu Asp
100 105 110
Val His Asp Leu Ala Glu His Val Ala Cys Val Val Gly Arg Asp Thr
115 120 125
Val Ala Thr Phe Lys Pro Asp Pro Glu Ser Leu Leu Ala Ala Val Glu
130 135 140
His Leu Asp Ala Thr Pro Glu Arg Thr Leu Phe Ile Gly Asp Ser Glu
145 150 155 160
Gly Asp Ala Glu Thr Ala Asp Arg Ala Gly Thr Arg Phe Ser Tyr Val
165 170 175
Gly Asp Gly Pro Thr Thr Tyr
180
<210> 80
<211> 212
<212> PRT
<213> Hydrogenivirga sp. 128-5-R1-1
<400> 80
Met Lys Ala Val Leu Phe Asp Leu Asp Gly Thr Leu Ile Asp Ser Ala
1 5 10 15
Asp Asp Ile Ala Leu Ala Leu Arg Leu Thr Leu Glu Glu Leu Gly Met
20 25 30
Glu Glu Lys Met Pro Pro Ser Val Arg Ser Leu Ile Gly Gly Gly Val
35 40 45
Lys Ala Leu Leu Glu Arg Val Leu Gly Asp Asp Phe Arg Glu Gln His
50 55 60
Val Lys Val Phe Arg Arg His Tyr Ile Gly Asn Pro Val Val Asn Thr
65 70 75 80
Thr Pro Tyr Pro Gly Val Met Glu Thr Leu Arg Ala Leu Arg Gly Arg
85 90 95
Gly Ile Asn Leu Val Val Val Thr Asn Lys Leu Glu Glu Leu Ser Val
100 105 110
Glu Ile Leu Lys Arg Leu Gly Met Leu Glu Phe Phe Asp Leu Val Val
115 120 125
Gly Gly Asp Thr Phe Pro Glu Lys Lys Pro Ser Pro Leu Pro Val Leu
130 135 140
Lys Ser Leu Glu Phe Val Gly Val Glu Ser Ser Gln Ala Leu Met Val
145 150 155 160
Gly Asp Thr Ser Ala Asp Ile Glu Ala Gly Lys Arg Ala Gly Val Arg
165 170 175
Thr Ala Leu Ala Ala Trp Gly Tyr Val Lys Leu Asn Ser Val Lys Pro
180 185 190
Asp Tyr Ile Phe Asn Ser Pro Ala Asp Ile Leu Ser Leu Ala Ser Gln
195 200 205
Val Ser Gly Ala
210
<210> 81
<211> 211
<212> PRT
<213> 嗜氢产氢杆菌(Hydrogenobacter hydrogenophilus)
<400> 81
Met Arg Leu Lys Leu Phe Ile Phe Asp Leu Asp Gly Thr Leu Ile Asp
1 5 10 15
Ser Tyr Met Asp Ile Gly Met Cys Val Asn Met Val Leu Gln Glu Met
20 25 30
Gly Arg Asn Pro Ile Asp Pro Glu Arg Val Lys Ala Trp Ile Gly Gly
35 40 45
Gly Ala Arg Arg Leu Leu Glu Lys Leu Phe Pro Asp Glu Glu Leu Asn
50 55 60
Thr Ala Leu Glu Leu Phe Arg Arg Phe Tyr Arg Glu Asn Pro Val Val
65 70 75 80
His Thr Lys Thr Tyr Glu Gly Ile Gln Glu Val Leu Ala Tyr Leu Arg
85 90 95
Ser Arg Gly Ser Phe Leu Ala Val Val Thr Asn Lys Met Glu Asp Leu
100 105 110
Ser Arg Glu Ile Leu Arg Arg Leu Glu Leu Leu Ser Tyr Phe Asp Val
115 120 125
Val Val Gly Gly Asp Thr Phe Pro Glu Lys Lys Pro Ser Pro Leu Pro
130 135 140
Ile Lys Gln Val Met Glu Arg Leu Arg Val Arg Pro Gln Glu Ser Val
145 150 155 160
Ile Val Gly Asp Thr Ser Ala Asp Ile Cys Ala Gly Lys Asp Ala Gly
165 170 175
Ile Trp Thr Ala Leu Ala Lys Trp Gly Tyr Val Arg Leu Asp Ser Val
180 185 190
Met Pro Asp Phe Phe Leu Ser Glu Pro Lys Asp Val Ile Asp Leu Phe
195 200 205
Glu Lys Val
210
<210> 82
<211> 262
<212> PRT
<213> 嗜氢产氢杆菌(Hydrogenobacter hydrogenophilus)
<400> 82
Met Asp Lys Val Glu Ser Phe Leu Lys Val Ala Lys Glu Ser Ala Leu
1 5 10 15
Ile Gly Gly Leu Ile Leu Lys Glu His Phe Gly Lys Leu Thr Gln Lys
20 25 30
Asp Val Gln Glu Lys Ser Glu Lys Asp Val Val Ser Phe Val Asp Lys
35 40 45
Ser Ser Glu Glu Arg Ile Arg Lys Tyr Ile Lys Ile Asn Phe Pro Asp
50 55 60
His Asp Val Val Gly Glu Glu Glu Gly Gly Thr Asp Asn Ser Asp Tyr
65 70 75 80
Val Trp Tyr Ile Asp Pro Leu Asp Gly Thr Lys Asn Tyr Ile Ala Gly
85 90 95
Phe Pro Ile Phe Gly Val Ser Val Gly Leu Thr Tyr Arg Lys Glu Pro
100 105 110
Ile Val Gly Ala Val Tyr Met Pro Leu Tyr Asp Thr Leu Tyr Trp Ala
115 120 125
Tyr Lys Gly Gly Gly Ala Tyr Lys Asn Gly Lys Ser Ile Glu Val Ser
130 135 140
Lys Arg Val Gln Val Lys Ser Phe Phe Ile Ala Tyr Gly Phe Pro Ser
145 150 155 160
Arg Ala Lys Arg Asn Leu Asp Ile Tyr Trp Asn Ile Phe Lys Glu Leu
165 170 175
Phe Glu Lys Val Gly Ala Met Arg Arg Pro Gly Ala Ala Ala Ile Asp
180 185 190
Leu Cys Phe Thr Ala Glu Gly Ile Phe Asp Gly Leu Ile Glu Phe Glu
195 200 205
Leu Asn Pro Trp Asp Val Cys Ala Gly Ile Leu Ile Leu Gln Glu Ala
210 215 220
Gly Gly Lys Val His Leu Thr Lys Asp Leu Thr Lys Gly Thr Asp Val
225 230 235 240
Ile Ala Gly Thr Pro Thr Ala Phe Pro Tyr Ile Glu Asn Thr Val Lys
245 250 255
Ser Asn Leu Glu Gly Leu
260
<210> 83
<211> 211
<212> PRT
<213> 嗜氢氢杆菌(Hydrogenobacter thermophilus)
<400> 83
Met Arg Leu Lys Leu Phe Ile Phe Asp Leu Asp Gly Thr Leu Ile Asp
1 5 10 15
Ser Tyr Met Asp Ile Gly Met Cys Val Asn Gly Val Leu Arg Ser Met
20 25 30
Gly Arg Gln Glu Ile Glu Pro Asp Ser Val Lys Asn Trp Ile Gly Gly
35 40 45
Gly Ala Arg Gly Leu Leu Glu Lys Leu Phe Pro Ser Glu Glu Ile Asp
50 55 60
Arg Ala Leu Glu Leu Phe Arg Lys Tyr Tyr Arg Glu Asn Pro Val Val
65 70 75 80
Tyr Thr Lys Ala Tyr Lys Gly Ile Arg Glu Val Leu Thr His Leu Lys
85 90 95
Ala Met Glu Lys Arg Leu Ala Val Val Thr Asn Lys Met Glu Asp Leu
100 105 110
Ser Val Glu Ile Leu Lys Arg Leu Asn Leu Tyr His Tyr Phe Asp Met
115 120 125
Val Val Gly Gly Asp Thr Phe Pro Glu Lys Lys Pro Ser Pro Val Pro
130 135 140
Ile Lys Glu Val Leu Arg Trp Leu Gly Val Asp Ala Lys Glu Ala Val
145 150 155 160
Met Ile Gly Asp Thr Asp Ala Asp Val Arg Ala Gly Lys Asp Ala Gly
165 170 175
Val Trp Thr Ala Leu Ala Asn Trp Gly Tyr Val Arg Met Asp Gly Val
180 185 190
Lys Pro Asp Phe Val Leu His Lys Pro Glu Asp Ile Ile Asn Leu Pro
195 200 205
Gly Arg Ala
210
<210> 84
<211> 247
<212> PRT
<213> 丁酸栖高温菌(Hyperthermus butylicus)
<400> 84
Met Ala Glu Ala Val Arg Ile Glu Leu Pro Ala Arg Val Cys Gly Leu
1 5 10 15
Ala Leu Asp Ile Asp Gly Thr Leu Thr Glu Lys Lys Arg Leu Gly Glu
20 25 30
Phe Asn Ile Ser Leu Glu Ala Val Glu Ala Val Arg Arg Ala Glu Ala
35 40 45
Ala Gly Ile Pro Val Met Leu Val Thr Gly Asn Ser Val Tyr Val Val
50 55 60
Ala Gly Val Ala Arg Tyr Ile Gly Ala Ser Gly Pro His Val Ala Glu
65 70 75 80
Asn Gly Cys Ile Val Tyr Asp Asn Gly Thr Ile Tyr Arg Val Cys Arg
85 90 95
Asp Thr Ala Arg Arg Ala Ala Arg Leu Ile Glu Glu Glu Leu Ala Gly
100 105 110
Val Leu Lys Pro Ser Trp Gln Asn Pro Cys Arg Leu His Asp Tyr Ala
115 120 125
Phe Ile Pro Lys Leu Leu Glu Pro Gly Glu Ala Leu Glu Ala Val Lys
130 135 140
Arg Val Leu Ser Ser Arg Gly Ile Asn Val Lys Val Gly Phe Ser Gly
145 150 155 160
Tyr Ala Ile His Leu Arg Pro Val Asp Ala Ser Lys Gly Arg Gly Leu
165 170 175
Lys Leu Ala Leu Lys Leu Arg Gly Leu Glu Pro Asp Cys Val Val Ala
180 185 190
Val Gly Asp Ser Val Ile Asp Leu Glu Met Lys Asp Ala Gly Val Ile
195 200 205
Leu Ala Ala Val Gly Asn Ala Asp Glu Lys Leu Lys Lys Asn Ala Asp
210 215 220
Ile Val Leu Pro Gly Glu Ser Gly Arg Ser Val Lys Ala Leu Ile Asp
225 230 235 240
Ala Ile Leu Ser Asn Arg Gly
245
<210> 85
<211> 267
<212> PRT
<213> Kosmotoga arenicorallina
<400> 85
Met Lys Met Ile Glu Ala Phe Val Ile Asp Ile Asp Gly Thr Phe Ile
1 5 10 15
Asp Ser Lys Gly Asn Ile Pro Gln Glu Asn Leu Asp Ala Leu Lys Glu
20 25 30
Ile Glu Glu Glu Gly Ile Met Ile Phe Phe Ala Ser Gly Arg Met Leu
35 40 45
Ser Ser Val Ile Lys Phe Gln Ser Ser Lys Leu Asn Lys Ile His Pro
50 55 60
Ile Ile Ala Tyr Asn Gly Ala Val Val Trp Asn Gly Arg Asp Val Ile
65 70 75 80
Phe Ser Lys Asn Ile Thr Thr Glu Met Ala Lys Asp Ile Val Ser Tyr
85 90 95
Ser Leu Ser Lys Gly Leu Tyr Ile Gln Ala Tyr Val Asn Asp Glu Leu
100 105 110
Val Val Pro Phe Asp Cys Glu Lys Ala Arg Glu Tyr Ala Lys His Ser
115 120 125
Ala Val Asp Phe Arg Val Glu Glu Lys Phe Ile Glu Phe Val Thr Lys
130 135 140
Asn Thr Pro Thr Lys Leu Leu Ile Ile Asp Thr Pro Glu Lys Val Glu
145 150 155 160
Val Leu Ser Ala His Phe Ala Ala Arg Tyr Asn His Leu Asn Val Phe
165 170 175
Arg Ser Phe Ala Thr Tyr Leu Asp Leu Leu Pro Glu Gly Val Asn Lys
180 185 190
Gly Leu Gly Leu Lys Tyr Leu Cys Glu Asp Leu His Val Asp Pro Ala
195 200 205
Cys Val Val Ala Phe Gly Asp Asn Asp Asn Asp Val Pro Leu Phe Glu
210 215 220
Glu Ala Gly Phe Ser Val Ala Val Ala Asn Ala Thr Pro Asn Ala Arg
225 230 235 240
Lys Ala Ala Asp Val Ile Ala Ser Ala Asn Tyr Glu Ala Gly Phe Gly
245 250 255
Arg Thr Ala Met Lys Leu Ile Lys Leu Leu Lys
260 265
<210> 86
<211> 265
<212> PRT
<213> Kosmotoga olearia
<400> 86
Met Ile Asn Gly Val Val Ile Asp Ile Asp Gly Thr Phe Ile Asp Ser
1 5 10 15
Glu Glu Lys Ile Pro Ile Glu Asn Leu Glu Val Phe Arg Glu Leu Glu
20 25 30
Ala Lys Gly Leu Arg Val Ile Phe Ala Ser Gly Arg Met Leu Thr Ser
35 40 45
Val Lys Asn Phe Ile Ser Lys Ile Ser Asp Lys Ala Tyr Pro Ile Ile
50 55 60
Ala Tyr Asn Gly Ala Val Val Tyr Val Asn Gly Glu Asn Ile Phe Asn
65 70 75 80
Gln Val Leu Leu Gln Asp Thr Ala Val Arg Ile Val Glu Arg Ala Leu
85 90 95
Ser Asn Asn Met Tyr Ile Gln Ala Tyr Val Asp Asp Arg Leu Val Val
100 105 110
Pro Lys Asp Cys Glu Glu Ala Arg Ser Tyr Ala Ser His Ser Gly Val
115 120 125
Asp Phe Met Val Val Glu Asp Leu Thr Asn Tyr Leu Ser Lys His Pro
130 135 140
Thr Ile Lys Leu Leu Met Ile Ala Pro Ser Glu Gln Ile Asp Asn Leu
145 150 155 160
Arg Leu Glu Phe Ser Glu Ile Phe Pro Glu Val Asp Phe Val Arg Ser
165 170 175
Phe Ser Thr Tyr Leu Asp Ile Val Pro Lys Gly Val Ser Lys Gly Lys
180 185 190
Ala Leu Glu Ile Leu Cys Lys His Leu Glu Ile Asp Ile Gly Lys Leu
195 200 205
Ile Ala Phe Gly Asp Asn Asp Asn Asp Ile Ser Leu Phe Glu Arg Cys
210 215 220
Gly Phe Ser Ile Ala Met Ala Asn Ala Thr Gln Arg Ala Lys Lys Ala
225 230 235 240
Ala Asp Val Ile Ala Pro Ser Asn Asp Glu Ala Gly Phe Ala Arg Val
245 250 255
Met Lys Lys Leu Leu Thr Leu Cys Asp
260 265
<210> 87
<211> 280
<212> PRT
<213> Kosmotoga olearia
<400> 87
Met Ile Lys Leu Ile Ala Ile Asp Leu Asp Gly Thr Leu Leu Asn Asn
1 5 10 15
Lys Lys Glu Ile Ser Leu Glu Asn Thr Ala Ala Leu Thr Glu Ala Met
20 25 30
Glu Lys Gly Leu His Val Ser Ile Phe Thr Gly Arg Ser Tyr Ile Ser
35 40 45
Gly Ser Glu Tyr Ala Lys Lys Leu Gly Leu Arg Val Pro Val Val Tyr
50 55 60
Gln Asn Gly Ala Leu Ile Ile Asn Ser Asp Asp Gly Asn Lys Gly Val
65 70 75 80
Ile Arg Lys Val Ile Leu Ser Ala Lys Arg Ala Arg Glu Ile Val Glu
85 90 95
Ala Ala Lys Lys Tyr Gly Leu Thr Tyr Ile Val Phe Thr Asn Phe Phe
100 105 110
Asp Leu Pro Asp Met Phe Met Glu Arg Ile Pro Glu Asn Ser Pro Phe
115 120 125
Lys Gly Tyr Phe Arg Ala Asn Leu Tyr Arg Ile Thr Ile Val Asn Asp
130 135 140
Pro Ala Asp Phe Ile Arg Asp Glu Gly Ile Ala Glu Val Ala Val Glu
145 150 155 160
Gly Pro Glu Asn Arg Ile Leu Ser Met Val Glu Glu Leu Gly Gln Pro
165 170 175
Met Asn Asp Leu Ser Ile Val Lys Asn Asn Arg Ile Asn Glu His Thr
180 185 190
Phe Tyr Glu Phe Phe Gly Pro Asn Val Gly Lys Asn Gln Ala Leu Asp
195 200 205
Tyr Val Met His His Leu Ala Leu Thr Pro Asp Glu Ile Ala Tyr Ile
210 215 220
Gly Asp Asn Tyr Asn Asp Val Glu Ile Met Lys Val Val Gly Leu Pro
225 230 235 240
Ile Ala Met Ala Asn Ala Pro Lys Glu Val Lys Asp Phe Ala Arg Tyr
245 250 255
Val Thr Thr Arg Thr Asn Asn Glu His Gly Val Ala Glu Ala Val Arg
260 265 270
Lys Ile Ile Arg Glu Glu Ile Arg
275 280
<210> 88
<211> 275
<212> PRT
<213> Marinitoga piezophila
<400> 88
Met His Asn Lys Thr Phe Ile Phe Asp Leu Asp Gly Thr Leu Leu Asn
1 5 10 15
Ser Asn Glu Ser Ile Ser Gln Arg Thr Val Glu Ser Ile Lys Lys Ile
20 25 30
Phe Glu Met Gly Ser Phe Ile Ile Ile Ala Ser Gly Arg Met Tyr Lys
35 40 45
Ser Thr Arg Leu Ile Val Glu Lys Tyr Met Pro Phe Leu His Asn Asn
50 55 60
Ile Pro Ile Val Ser Tyr Asn Gly Ala Tyr Val Val Ser His Thr Gly
65 70 75 80
Glu Val Val Phe Glu Ser Asp Ile Leu Lys Asp Leu Ala Ile Lys Val
85 90 95
Ile Glu Asp Leu Lys Lys His Asp Thr His Val Gln Ile Tyr Leu Asn
100 105 110
Asp Asp Leu Ile Thr Asp Lys Asp Asn Asp Glu Ile Lys Gln Tyr Ala
115 120 125
Lys His Ser Gly Val Glu Tyr Lys Ile Val Lys Asp Ile Val Glu His
130 135 140
Ile Lys Gln His Gly Glu Pro Thr Lys Ile Leu Ala Ile Asn Gln Pro
145 150 155 160
Glu Lys Leu Asp Ile Ile Gln Lys Ser Met Thr Glu Lys Tyr Gly Asn
165 170 175
Thr Leu Asn Ile Val Arg Ser Phe Ser Ile Tyr Leu Asp Phe Leu Asn
180 185 190
Lys Asp Ser Ser Lys Gly Leu Ala Leu Lys Lys Leu Lys Glu Ile Tyr
195 200 205
Arg Phe Asn Leu Glu Glu Ala Tyr Ile Phe Gly Asp Ser Glu Asn Asp
210 215 220
Ile Ser Met Leu Ser Leu Ser Lys Asn Ser Tyr Ala Met Ala Asn Ala
225 230 235 240
Asn Glu His Val Lys Glu Ser Ala Arg Asn Ile Thr Leu Ser Asn Asp
245 250 255
Glu Glu Gly Val Ala Ile Val Leu Glu Lys Ile Ile Ser Asn Phe Ser
260 265 270
Asn Ser Asn
275
<210> 89
<211> 213
<212> PRT
<213> Marinitoga piezophila
<400> 89
Met Met Lys Tyr Lys Tyr Leu Phe Phe Asp Met Asp Gly Thr Leu Phe
1 5 10 15
Glu Thr Ser Arg Gly Ile Ile Tyr Ser Leu Gln Lys Ala Tyr Lys Ile
20 25 30
Asn Asn Leu Thr Pro Leu Pro Glu Asp Lys Ile Lys Lys Leu Ile Gly
35 40 45
Pro Thr Leu Asp Glu Ile Met Asn Ile Leu Phe Gly Glu Glu Asn Thr
50 55 60
Glu Leu Lys Met Lys Val Arg Glu Asp Phe Arg Met Thr Tyr Ala Lys
65 70 75 80
Glu Gly Val Phe Met Leu Asp Leu Tyr Pro Glu Val Met Asp Thr Leu
85 90 95
Glu Lys Leu Tyr Ser Lys Asn Arg Ile Met Tyr Ile Val Thr Ser Lys
100 105 110
Pro Glu Ile Phe Ala Ser Gln Ile Leu Glu Lys Phe Asn Met Ser Arg
115 120 125
Phe Phe Lys Lys Ile Cys Gly Val Pro Leu Asp Gly Lys Ile Pro Pro
130 135 140
Lys Ser Glu Arg Leu Lys Asn Leu Ile Ile Glu Glu Asn Ile Pro Leu
145 150 155 160
Lys Glu Gly Leu Met Ile Gly Asp Thr Leu Ser Asp Ala Lys Ala Ala
165 170 175
Trp Glu Asn Asn Val Asp Phe Ala Trp Ile Ser Phe Gly Phe Gly His
180 185 190
Glu Asn Glu Ile Lys Lys Tyr Asp Tyr Lys Ile Asn Asn Phe Ser Gln
195 200 205
Leu Leu Asn Leu Lys
210
<210> 90
<211> 217
<212> PRT
<213> Marinitoga piezophila
<400> 90
Met Asn Phe Ile Phe Asp Leu Asp Gly Thr Leu Ile Asn Thr Glu Asn
1 5 10 15
Ile Ala Ile Pro Ala Ile Arg Gln Leu Leu Lys Asp Leu Gly Tyr Asn
20 25 30
Pro Asn Ile Glu Lys Glu Glu Ile Leu Gln Tyr Ile Gly Tyr Thr Ile
35 40 45
Asp Asp Ile Phe Tyr Gly Leu Leu Asn Thr Lys Asp Pro Glu Ile Ile
50 55 60
Glu Lys Ala Ile Lys Leu Leu Asp Lys Tyr Glu Ile Glu Ile Ile Glu
65 70 75 80
Lys Leu Pro Lys Asn Glu Ile Phe Phe Asp Gly Ala Ile Glu Val Leu
85 90 95
Lys Thr Leu Lys Asn Glu Asn His Thr Leu Tyr Ile Leu Ser Asn Cys
100 105 110
Asn Lys Lys Tyr Leu Asn Ala Leu Leu Glu Lys Glu Leu Asn Lys Tyr
115 120 125
Ile Asp Phe Pro His Cys Ser Glu Met Tyr Asn Trp Ala Glu Lys Asp
130 135 140
Tyr Val Leu Lys Leu Ile Thr Asn Gly Lys Lys Asp Phe Val Met Ile
145 150 155 160
Gly Asp Arg His Lys Asp Val Asp Ala Ala Lys Lys Asn Gly Ile Leu
165 170 175
Ser Val Gly Cys Thr Tyr Gly Tyr Ala Leu Glu Glu Val Lys Asp Ala
180 185 190
Asp Phe Ile Ile Tyr Asp Ile Lys Glu Leu Leu Thr Ile Lys Asn Lys
195 200 205
Ile Ile Glu Ser Ala Tyr Asn Thr Ile
210 215
<210> 91
<211> 258
<212> PRT
<213> Meiothermus cerbereus
<400> 91
Met Asp Leu Arg Val Tyr Leu Gln Thr Ala Leu Asp Ala Ala Tyr Leu
1 5 10 15
Ala Lys Gly Ile His Gln Tyr Tyr Gln Glu Lys Gly Phe Thr Leu Ser
20 25 30
Ser Lys Ser Thr Pro Thr Asp Leu Val Thr Gln Ala Asp His Glu Ser
35 40 45
Glu Ala Ala Ile Arg Glu Leu Ile Ala Ala Arg His Pro Asp His Val
50 55 60
Val Leu Gly Glu Glu Gln Gly Gln Asp Arg Glu Gly Ala Phe Arg Trp
65 70 75 80
Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe Pro
85 90 95
Phe Tyr Ala Val Ser Ile Gly Leu Glu Ala His Gly Glu Val Val Leu
100 105 110
Gly Val Ile Leu Asp Thr Ala Arg Gly Asp Leu Phe Thr Ala Thr Lys
115 120 125
Gly Gly Gly Ala Phe Met Asn Gly Arg Pro Ile Arg Val Ser Pro Arg
130 135 140
Ala Thr Leu Val Gly Ser Leu Leu Ala Thr Gly Phe Pro Tyr Asp Val
145 150 155 160
Ser Lys Asp Thr Glu Asn Leu Val Tyr Phe Gln Arg Ala Leu Thr Arg
165 170 175
Gly Leu Met Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala Tyr
180 185 190
Val Ala Ala Gly Arg Leu Asp Gly Phe Trp Glu Val Lys Leu Asn Pro
195 200 205
Trp Asp Val Ala Ala Gly Trp Leu Ile Ile Arg Glu Ala Gly Gly Thr
210 215 220
Val Thr Gly Met Glu Gly Glu Ala Tyr Arg Leu Gly Asn Arg Tyr Leu
225 230 235 240
Val Ala Ser Asn Gly Leu Ile His Gln Pro Leu Leu Asp Thr Ile His
245 250 255
Gly Arg
<210> 92
<211> 216
<212> PRT
<213> Meiothermus cerbereus
<400> 92
Met Lys Leu Lys Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp
1 5 10 15
Thr Asp Arg Leu His Glu Gln Ala Trp Leu Glu Gly Leu Ser Arg Tyr
20 25 30
Gly Leu Gln Gly Asp His Thr Phe Tyr Gln Thr Gln Ile Ser Gly Gly
35 40 45
Leu Asn Pro Glu Ile Val Gln Arg Leu Leu Pro Gln Leu Ser Gln Ala
50 55 60
Glu Ala Glu Ala Phe Leu Glu Gln Lys Glu Ala Arg Phe Arg Glu Leu
65 70 75 80
Ala Ser Glu Val Gln Pro Leu Pro Gly Leu Gly Thr Leu Trp Asp Trp
85 90 95
Ala Gln Glu Gln Asn Leu Arg Arg Ala Leu Val Ser Asn Ala Pro Arg
100 105 110
Glu Asn Ala Gln Tyr Leu Leu Lys Arg Leu Gly Leu Val Phe Asp His
115 120 125
Ile Val Leu Ser Glu Glu Leu Pro Ala Gly Lys Pro Asp Pro Leu Pro
130 135 140
Tyr Arg Thr Ala Leu Gln Ala Leu Asn Ile Gly Pro Ser Glu Ala Leu
145 150 155 160
Ala Phe Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly
165 170 175
Ile Arg Thr Val Ala Leu Thr Thr Gly His Pro Ala His Ala Leu Glu
180 185 190
Gln Ala Gly Ala Phe Leu Cys Ile Pro Asn Phe Ala Asp Pro Arg Leu
195 200 205
Trp Ala Tyr Leu His Lys Met Gly
210 215
<210> 93
<211> 258
<212> PRT
<213> Meiothermus chliarophilus
<400> 93
Met Asp Leu Arg Pro Phe Leu Glu Thr Ala Leu Ala Ala Ala Tyr Val
1 5 10 15
Ala Arg Gly Ile His Gln Leu Tyr Gln Asp Lys Gly Phe Thr Gln Ser
20 25 30
Thr Lys Ser Thr Pro Thr Asp Val Val Thr Gln Ala Asp Lys Glu Ala
35 40 45
Glu Ala Ala Ile Arg Ala Leu Ile Glu Gln Arg His Pro Gly His Val
50 55 60
Val Leu Gly Glu Glu Glu Gly Gln Gln Gly Glu Gly Glu Tyr Arg Trp
65 70 75 80
Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe Pro
85 90 95
Phe Tyr Cys Val Ser Ile Gly Leu Glu Val Arg Gly Glu Val Met Val
100 105 110
Gly Val Val Leu Asp Thr Ala Arg Gly Asp Leu Phe Thr Ala Thr Arg
115 120 125
Gly Gly Gly Ala Tyr Phe Asn Gly Arg Pro Met Arg Val Ser Arg Ser
130 135 140
Pro Lys Leu Leu Gly Ser Leu Ile Ala Thr Gly Phe Pro Tyr Asp Val
145 150 155 160
Ala Arg Asp Arg Glu Asn Leu Thr Tyr Leu Glu Arg Val Leu Phe Lys
165 170 175
Gly Ile Thr Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala Tyr
180 185 190
Val Ala Cys Gly Arg Leu Asp Gly Phe Trp Glu Val Lys Leu Asn Pro
195 200 205
Trp Asp Val Ala Ala Gly Trp Leu Leu Val Glu Glu Ala Gly Gly Arg
210 215 220
Ile Thr Gly Ile His Gly Glu Pro Tyr Arg Met Gly Asn Arg Tyr Leu
225 230 235 240
Met Ala Ser Asn Gly His Ile His Glu Glu Leu Leu Ala Thr Ile His
245 250 255
Gly Arg
<210> 94
<211> 262
<212> PRT
<213> Meiothermus chliarophilus
<400> 94
Met Asp Leu Asp Trp Thr Leu Val His Pro Ser Gln Gly Val Pro Glu
1 5 10 15
Ser Ala Trp Arg Ala Val Glu Glu Ala Arg Ala Ala Gly Val Arg Leu
20 25 30
Ala Val Ala Thr Gly Arg Pro Phe Gly Gly Phe Gly Leu Glu Tyr Ala
35 40 45
Leu Arg Met Asn Pro Glu Gly Tyr His Ala Phe Ser Asn Gly Ser Leu
50 55 60
Ile Ala Arg Gly Ala Glu Leu Val His Arg Val Ala Met Pro Glu Ala
65 70 75 80
Thr Tyr Arg Arg Met Val Leu His Ser Arg Glu Gln Gly Leu Pro Phe
85 90 95
Tyr Val Ser Gly Ala Ser Gly Arg Leu Tyr Ser Glu Asn Pro Pro Arg
100 105 110
Glu Leu His Arg Phe Ala Leu Arg Met Gly Val Gly Tyr Glu Arg Val
115 120 125
Asp Leu Leu Glu Leu Pro Glu Pro Cys Val Gly Gly Val Phe Val Ile
130 135 140
Thr Gly Gly Leu Trp Asp Ser Leu Arg Pro Ser Leu Thr Ala Val Glu
145 150 155 160
Gly Leu Asp Trp Leu Glu Tyr Val Val Gly Glu Gly Glu Val Val Ala
165 170 175
Val Ala Asp Pro Lys Gly Val Ser Lys Ala Ser Ala Leu Arg Trp Met
180 185 190
Ala Glu Arg Tyr Gly Leu Ala Met Glu Glu Val Ala Met Ile Gly Asp
195 200 205
Ser Leu Asn Asp Leu Glu Ala Ile Arg Gln Val Gly Leu Gly Ile Ala
210 215 220
Met Gly Asn Ala Glu Pro Ala Ile Leu Glu Ala Ala Asp Ala Val Val
225 230 235 240
Gly Arg Leu Glu Glu Asp Gly Phe Ala Gln Ala Val Arg Leu Cys Leu
245 250 255
Glu Pro Ser Glu Val Arg
260
<210> 95
<211> 258
<212> PRT
<213> Meiothermus ruber
<400> 95
Met Asp Leu Arg Ala Tyr Leu Gln Thr Ala Leu Asp Ala Ala Tyr Leu
1 5 10 15
Ala Lys Gly Ile His Gln Tyr Tyr Gln Glu Lys Gly Phe Thr Gln Ser
20 25 30
Thr Lys Ser Thr Pro Thr Asp Leu Val Thr Gln Ala Asp His Glu Ser
35 40 45
Glu Ala Ala Ile Arg Glu Leu Ile Ala Ser Arg His Pro Asp His Val
50 55 60
Val Leu Gly Glu Glu Gln Gly Gln Asp Lys Glu Gly Ala Phe Arg Trp
65 70 75 80
Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe Pro
85 90 95
Phe Tyr Ala Val Ser Ile Gly Leu Glu Ala His Gly Glu Val Val Leu
100 105 110
Gly Val Val Leu Asp Thr Ala Arg Gly Glu Leu Phe Thr Ala Thr Lys
115 120 125
Gly Gly Gly Ala Tyr Leu Asn Gly Arg Pro Ile Arg Val Ser Thr Arg
130 135 140
Ser Thr Leu Val Gly Ser Leu Leu Ala Thr Gly Phe Pro Tyr Asp Val
145 150 155 160
Ser Lys Asp Thr Glu Asn Leu Val Tyr Phe Gln Arg Ala Leu Thr Lys
165 170 175
Gly Leu Met Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala Tyr
180 185 190
Val Ala Ala Gly Arg Leu Asp Gly Phe Trp Glu Val Lys Leu Asn Pro
195 200 205
Trp Asp Val Ala Ala Gly Trp Leu Ile Val Ser Glu Ala Gly Gly Arg
210 215 220
Ile Thr Gly Leu Gln Gly Glu Asp Tyr Arg Leu Gly Asn Arg Tyr Leu
225 230 235 240
Val Ala Ser Asn Gly Leu Ile His Gly Pro Leu Leu Asp Thr Ile His
245 250 255
Gly Arg
<210> 96
<211> 272
<212> PRT
<213> Meiothermus ruber
<400> 96
Met Ile Arg Leu Val Ala Leu Asp Leu Asp Gly Thr Phe Tyr Ala Gly
1 5 10 15
Arg Ser Leu Gly Val Pro Ala Ser Ala Trp Glu Ala Val Glu Lys Gly
20 25 30
Arg Arg His Gly Leu Arg Phe Ala Val Cys Thr Gly Arg Pro Gln Gly
35 40 45
Gly Tyr Gly Leu Glu Tyr Ala Lys Arg Leu Glu Pro Asn Gly Ala His
50 55 60
Val Phe Asn Asp Gly Ala Ser Val Cys Asp Ala Thr Gly Arg Ser Leu
65 70 75 80
Gln Ala Asp Pro Leu Pro His Leu Ser Glu Leu Val Gly Leu Ala Arg
85 90 95
Ala His Ala Leu Pro Phe Asp Leu Met Gly Ala Glu Gly Gly Arg Tyr
100 105 110
Tyr Glu Glu Gly Leu Met Pro Pro Glu Leu Leu Ser His Ile Glu Thr
115 120 125
Thr Gly Val Glu Ala Arg Ser Ala Arg Leu Glu Glu Ile Glu Glu Thr
130 135 140
Leu Val Arg Leu Trp Phe Val Val Gly Asp Leu Gly Leu Trp Glu Ser
145 150 155 160
Val Lys Pro Glu Leu Val Ala Leu Pro Ser Ile Asp Leu Ala Glu Tyr
165 170 175
Ile Ser Pro Arg Glu Val Ile Ala Gly Val Ile Arg Lys Gly Val Ser
180 185 190
Lys Ala Thr Gly Leu Arg Trp Leu Ala Gln Tyr Tyr Gly Leu Ser Leu
195 200 205
Ser Glu Ile Ala Met Ile Gly Asp Ser His Asn Asp Leu Glu Ala Ile
210 215 220
Arg Glu Ala Gly Leu Gly Ile Ala Met Gly Asn Ala Val Glu Ala Ile
225 230 235 240
Arg Ala Ala Ala Arg His Ile Thr Gly His Val Arg Glu Asp Gly Phe
245 250 255
Ala Glu Ala Val Glu Tyr Ile Leu Ala Tyr Asn Arg Gln Asn Gln Leu
260 265 270
<210> 97
<211> 278
<212> PRT
<213> Meiothermus ruber
<400> 97
Met Leu Gln Lys Ile Ser Arg Leu Pro Ile Gln Leu Val Leu Ile Asp
1 5 10 15
Ile Asp Gly Thr Leu Phe Gly Pro Gln Gly Val Pro Glu Cys Ala Trp
20 25 30
Glu Ala Ala Gln Arg Ala Arg Ala Ala Gly Leu His Leu Ser Ile Cys
35 40 45
Thr Gly Arg Pro Gly Arg Gly Phe Ala Leu Glu Tyr Ala Lys Arg Leu
50 55 60
Asp Pro Ala Gly Leu His Ile Phe Glu Ser Gly Ala Val Val Val Ser
65 70 75 80
Gly Gln Gly Glu Val Val Gln Ala Ser Thr Leu Pro Pro Ser Val Tyr
85 90 95
Gln Arg Leu Leu Ala Leu Ser Arg Ala Tyr Gly Leu Pro Phe Glu Val
100 105 110
Tyr Thr Ala Glu Gly Gly Phe Tyr Arg Glu Ser Gln His Pro Asp Leu
115 120 125
Val Phe His Glu Ser Met Leu Gly Cys Pro Ala Val Val Cys Gly Leu
130 135 140
Asp Asp Val Gly Ala Gln Val Val Arg Val Gln Phe Val Trp Arg Ala
145 150 155 160
Ser Pro Ala Trp Gln Ala Val Arg Ala Gln Ile Ala Gln Met Thr Glu
165 170 175
Val Glu Leu His Glu Ala Thr Ser Pro Gly Met Pro Gly Val Gly Phe
180 185 190
Ser Ser Val Thr Ala Ala Gly Val Ser Lys Arg Ala Ala Ala Glu Trp
195 200 205
Val Ala Ala Arg Leu Asp Leu Asp Leu Ser Arg Cys Ala Met Val Gly
210 215 220
Asp Gly Glu Asn Asp Leu Glu Leu Ile Gln Ala Ala Gly Leu Gly Ile
225 230 235 240
Ala Met Gly Asn Ala Pro Glu Arg Val Lys Gln Ala Ala Gln Arg Val
245 250 255
Val Gly Pro Val Glu Ala Cys Gly Leu Glu Gln Ala Leu Glu Ile Ile
260 265 270
Gln Asn His Ala Arg Ser
275
<210> 98
<211> 216
<212> PRT
<213> Meiothermus ruber
<400> 98
Met Lys Leu Arg Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp
1 5 10 15
Thr Asp Arg Leu His Glu Gln Ala Trp Leu Glu Gly Leu Ser Arg Tyr
20 25 30
Gly Ile Gln Gly Asp His His Phe Tyr Gln Thr Gln Ile Ser Gly Gly
35 40 45
Leu Asn Pro Glu Ile Val Gln Arg Leu Leu Pro Gln Leu Ser Glu Ala
50 55 60
Glu Gly Gln Arg Phe Ile Glu Gln Lys Glu Ala Arg Phe Arg Glu Leu
65 70 75 80
Ala Thr Thr Val Gln Pro Leu Pro Gly Leu Arg Val Leu Trp Asn Trp
85 90 95
Ala Gly Glu Arg Gly Leu Gly Arg Ala Leu Val Ser Asn Ala Pro Arg
100 105 110
Gly Asn Ala Leu Tyr Leu Leu Glu Arg Leu Gly Leu Val Phe Asp His
115 120 125
Ile Val Leu Ser Glu Asp Leu Pro Ala Gly Lys Pro Asp Pro Leu Pro
130 135 140
Tyr Arg Met Ala Leu Gln His Leu Asn Leu Ala Pro Gln Glu Ala Leu
145 150 155 160
Ala Phe Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly
165 170 175
Leu Pro Thr Val Ala Leu Thr Thr Gly His Pro Pro Glu Ala Leu Ala
180 185 190
Gln Ala Gly Ala Phe Leu Cys Ile Pro Asp Tyr Thr Asp Pro Arg Leu
195 200 205
Trp Asp Trp Leu Arg Ala Leu Gly
210 215
<210> 99
<211> 258
<212> PRT
<213> Meiothermus ruber
<400> 99
Met Asp Leu Arg Pro Tyr Leu Glu Ala Ala Leu Asp Ala Ala Tyr Leu
1 5 10 15
Ala Lys Gly Ile His Gln Tyr Tyr Gln Glu Lys Gly Phe Thr His Ser
20 25 30
Thr Lys Ser Thr Pro Thr Asp Val Val Thr Gln Ala Asp His Glu Ala
35 40 45
Glu Ala Ala Ile Arg Ala Leu Ile Ala Glu Arg Phe Pro Asp His Val
50 55 60
Val Leu Gly Glu Glu Gln Gly Gln Glu Gly Glu Gly Glu Phe Arg Trp
65 70 75 80
Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe Pro
85 90 95
Phe Tyr Gly Val Ser Ile Gly Leu Glu Val Arg Gly Glu Val Val Leu
100 105 110
Gly Val Val Leu Asp Thr Ala Arg Gly Asp Leu Phe Thr Ala Thr Lys
115 120 125
Gly Gly Gly Ala Tyr Leu Asn Gly Arg Pro Leu Arg Val Ser Gln Arg
130 135 140
Thr Thr Leu Val Gly Ser Leu Leu Ala Thr Gly Phe Pro Tyr Asp Val
145 150 155 160
Ala Arg Asp Pro Glu Asn Leu Thr Tyr Phe Gln Arg Ala Leu Ala Lys
165 170 175
Gly Leu Thr Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala Asn
180 185 190
Val Ala Ala Gly Arg Leu Asp Gly Phe Trp Glu Val Lys Leu Asn Pro
195 200 205
Trp Asp Val Ala Ala Gly Trp Leu Leu Ile Thr Glu Ala Gly Gly Gln
210 215 220
Val Thr Gly Phe Gln Gly Glu Ala Tyr Arg Leu Gly Asn Arg Tyr Leu
225 230 235 240
Val Ala Ser Asn Gly Arg Ile His Gln Ala Leu Leu Glu Ala Leu Gln
245 250 255
Gly Arg
<210> 100
<211> 258
<212> PRT
<213> Meiothermus silvanus
<400> 100
Met Asp Leu Gln Arg Tyr Leu Glu Ala Ala Leu Asp Ala Ala Tyr Leu
1 5 10 15
Ala Arg Gly Ile His Leu Tyr Tyr Arg Glu Lys Gly Phe Ala Leu Glu
20 25 30
Ser Lys Thr Ser Pro Thr Asp Leu Val Thr Gln Ala Asp Arg Glu Ser
35 40 45
Glu Glu Ala Ile Arg Ser Leu Leu Leu Glu Arg Phe Pro Asp His Val
50 55 60
Val Leu Gly Glu Glu Gly Gly Gln Glu Gly His Ser Asp Phe Arg Trp
65 70 75 80
Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe Pro
85 90 95
Phe Tyr Ala Val Ser Ile Ala Leu Glu Val Gly Gly Glu Val Val Val
100 105 110
Gly Ala Val Leu Asp Thr Ala Arg Gly Glu Leu Phe Thr Ala Leu Lys
115 120 125
Gly Glu Gly Ala Phe Gln Asn Gly Arg Pro Ile Arg Val Ser Ser Thr
130 135 140
Ala Thr Leu Ile Gly Ser Leu Leu Ala Thr Gly Phe Pro Tyr Asp Val
145 150 155 160
Ala Lys Asp Ala Glu Asn Leu Thr Tyr Phe Gln Arg Ala Leu Ala Lys
165 170 175
Gly Leu Thr Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala Tyr
180 185 190
Val Ala Ala Gly Arg Leu Glu Gly Phe Trp Glu Val Lys Leu Asn Pro
195 200 205
Trp Asp Val Ala Ala Gly Trp Leu Leu Ile Thr Glu Ala Gly Gly Thr
210 215 220
Val Ser Gly Ile Gln Gly Glu Pro Tyr Arg Leu Gly Asn Arg Tyr Leu
225 230 235 240
Val Ala Ser Asn Gly Lys Ile His Glu Gln Leu Leu Asp Thr Leu His
245 250 255
Gly Arg
<210> 101
<211> 213
<212> PRT
<213> Meiothermus silvanus
<400> 101
Met Arg Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asn Thr Asp
1 5 10 15
Arg Leu His Glu Gln Ala Trp Leu Glu Thr Leu Arg Phe Tyr Gly Ile
20 25 30
Glu Gly Asp His His Phe Tyr Gln Thr Gln Ile Ser Gly Gly Leu Asn
35 40 45
Pro Glu Ile Val Arg Arg Leu Leu Pro Gln Leu Ser Glu Ala Glu Gly
50 55 60
Glu Ala Phe Ile Ala Arg Lys Glu Arg Arg Phe Arg Glu Leu Ala Gln
65 70 75 80
Asp Leu Arg Ala Leu Pro Gly Leu Asp Ala Leu Leu Ala Trp Ala Arg
85 90 95
Arg Lys Lys Leu Leu Thr Gly Leu Val Thr Asn Ala Pro His Glu Asn
100 105 110
Ala Arg His Val Thr Gln Ala Leu Gly Leu Ser Phe Asp Val Val Val
115 120 125
Leu Ala Glu Glu Leu Ala Ala Gly Lys Pro Asp Pro Leu Pro Tyr Arg
130 135 140
Val Ala Leu Glu Arg Leu Asp Leu Gly Ala Gln Glu Ala Leu Ala Phe
145 150 155 160
Glu Asp Ser Pro Ala Gly Val Lys Ala Ala Val Gly Ala Gly Ile Pro
165 170 175
Thr Ile Gly Leu Thr Thr Gly His Pro Pro Glu Ala Leu Lys Ala Ala
180 185 190
Gly Ala Phe Leu Leu Ile Ala Asp Phe Thr Asp Pro Gln Leu Trp Lys
195 200 205
Tyr Leu Glu Arg Ser
210
<210> 102
<211> 258
<212> PRT
<213> Meiothermus taiwanensis
<400> 102
Met Asp Leu Arg Ala Tyr Leu Gln Thr Ala Leu Asp Ala Ala Tyr Leu
1 5 10 15
Ala Lys Gly Ile His Gln Tyr Tyr Gln Glu Lys Gly Phe Thr Gln Ser
20 25 30
Ser Lys Ser Thr Pro Thr Asp Leu Val Thr Gln Ala Asp His Glu Ser
35 40 45
Glu Ala Ala Ile Arg Glu Leu Ile Ala Ala Arg His Pro Asp His Val
50 55 60
Val Leu Gly Glu Glu Gln Gly Gln Asp Lys Glu Gly Ala Phe Arg Trp
65 70 75 80
Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe Pro
85 90 95
Phe Tyr Ala Val Ser Ile Gly Leu Glu Ala His Gly Glu Val Val Leu
100 105 110
Gly Val Val Leu Asp Thr Ala Arg Gly Glu Leu Phe Thr Ala Thr Lys
115 120 125
Gly Gly Gly Ala Tyr Leu Asn Gly Arg Pro Ile Arg Val Ser Thr Arg
130 135 140
Ser Thr Leu Val Gly Ser Leu Leu Ala Thr Gly Phe Pro Tyr Asp Val
145 150 155 160
Ser Lys Asp Thr Glu Asn Leu Thr Tyr Phe Gln Arg Ala Leu Thr Lys
165 170 175
Gly Leu Met Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala Tyr
180 185 190
Val Ala Ala Gly Arg Leu Asp Gly Phe Trp Glu Val Lys Leu Asn Pro
195 200 205
Trp Asp Val Ala Ala Gly Trp Leu Ile Val Ser Glu Ala Gly Gly Arg
210 215 220
Val Ser Gly Leu Gln Gly Glu Asp Tyr Arg Leu Gly Asn Arg Tyr Leu
225 230 235 240
Val Ala Ser Asn Gly Leu Ile His Gln Pro Leu Leu Asp Thr Leu His
245 250 255
Gly Arg
<210> 103
<211> 216
<212> PRT
<213> Meiothermus taiwanensis
<400> 103
Met Lys Leu Arg Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp
1 5 10 15
Thr Asp Arg Leu His Glu Gln Ala Trp Leu Glu Gly Leu Ser Arg Tyr
20 25 30
Gly Ile Gln Gly Asp His Arg Phe Tyr Gln Ala Gln Ile Ser Gly Gly
35 40 45
Leu Asn Pro Glu Ile Val Ala Arg Leu Leu Pro Gln Leu Ser Pro Asp
50 55 60
Glu Gln Val Ala Phe Ile Glu Gln Lys Glu Ala Arg Phe Arg Glu Leu
65 70 75 80
Ala Ser Glu Val Gln Pro Leu Pro Gly Leu Arg Val Leu Trp Asp Trp
85 90 95
Ala Gln Ser Gln Gly Leu Arg Arg Ala Leu Val Ser Asn Ala Pro Arg
100 105 110
Glu Asn Ala His Tyr Leu Leu Glu Arg Leu Gly Leu Met Phe Asp Ala
115 120 125
Ile Val Leu Ser Glu Asp Leu Pro Ala Gly Lys Pro Asp Pro Leu Pro
130 135 140
Tyr Arg Thr Ala Leu Gln His Leu Asn Ile Gly Pro Gln Glu Ala Leu
145 150 155 160
Ala Phe Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly
165 170 175
Leu Arg Thr Val Ala Leu Thr Thr Gly His Pro Pro His Ala Leu Glu
180 185 190
Gln Ala Gly Ala Phe Leu Cys Ile Pro Asp Phe Thr Asp Pro Arg Leu
195 200 205
Trp Asp Trp Leu Gln Lys Met Gly
210 215
<210> 104
<211> 271
<212> PRT
<213> Meiothermus timidus
<400> 104
Met Ile Gly Leu Val Leu Ile Asp Val Asp Gly Thr Leu Tyr Gly Pro
1 5 10 15
Asp Gly Val Pro Glu Cys Ala Trp Glu Ala Thr Gln Lys Ala Arg Ala
20 25 30
Ala Gly Ile His Leu Ala Leu Cys Thr Gly Arg Pro Gly Arg Gly Phe
35 40 45
Ala Leu Glu Tyr Ala Arg Arg Ile Asp Pro Glu Gly Leu His Ile Phe
50 55 60
Glu Ser Gly Ala Val Val Val Ala Gly Asn Gly Gln Val Ile Lys Ser
65 70 75 80
Ser Pro Leu Ser Pro Glu Ala Tyr Arg Glu Leu Leu Ala Leu Ser Arg
85 90 95
Glu Tyr Ala Ile Pro Phe Glu Val Tyr Thr Ala Asp Gly Gly Phe Phe
100 105 110
Ile Glu Gln Asn His Pro Asp Leu Glu Ala His Gln Arg Met Leu Gly
115 120 125
Phe Met Ala Lys Val Ala Pro Leu Asp Gln Pro Pro Gly Glu Val Ile
130 135 140
Arg Val Gln Tyr Val Thr Pro Leu Glu Gly Gln Trp Gln Glu Val Arg
145 150 155 160
Gly Arg Val Gln Arg Ile Glu Gly Ala Glu Leu His Glu Ala Thr Ser
165 170 175
Pro Gly Val Pro Gly Ile Gly Phe Asn Ser Val Thr Ala Ala Gly Val
180 185 190
Ser Lys Leu Ser Ala Ala Arg Trp Val Ala Glu Arg Tyr Gly Leu Gly
195 200 205
Leu Glu His Cys Ala Met Val Gly Asp Gly Glu Asn Asp Leu Glu Leu
210 215 220
Ile Leu Ala Ala Gly Val Gly Ile Ala Met Gly Asn Ala Pro Gln Ser
225 230 235 240
Val Lys Glu Arg Ser Lys Arg Val Val Gly Arg Val Glu Glu Cys Gly
245 250 255
Leu Ala Gln Ala Leu Glu Trp Val Ala Ala Ala Glu Gly Arg Ser
260 265 270
<210> 105
<211> 214
<212> PRT
<213> Meiothermus timidus
<400> 105
Met Arg Pro Thr Thr Leu Leu Phe Asp Leu Asp Gly Thr Leu Leu Asp
1 5 10 15
Thr Glu Pro Gly Ile Leu Ala Cys Leu Arg His Thr Leu Glu His Phe
20 25 30
Gly Leu Pro Thr Gln Gly Asp Ala Glu Leu Arg Arg Phe Ile Gly Pro
35 40 45
Pro Leu Glu Gln Ala Trp Arg Glu Leu Val Gly Ala Ala His Val Glu
50 55 60
Glu Ala Val Glu Ile Tyr Arg Arg Cys Tyr Asp Leu Lys Gly Lys Phe
65 70 75 80
Lys Ala Ala Val Phe Glu Gly Ile Glu Ala Ala Leu His Glu Leu Ala
85 90 95
Tyr His His Thr Leu Ile Val Ala Thr Ser Lys Arg Gln Val Phe Ala
100 105 110
Glu Glu Met Leu Glu Arg Phe Gly Leu Ser Ser Tyr Phe Lys Ala Val
115 120 125
Tyr Gly Val Thr Pro Pro Gln Leu Ser Glu Pro Lys Ala His Leu Ile
130 135 140
Gly Arg Ile Leu Arg Asp His Gly Leu Ser Pro Gln Gln Ala Val Met
145 150 155 160
Val Gly Asp Arg Glu Phe Asp Val Ile Gly Ala Lys Ala Asn Gly Met
165 170 175
Ala Cys Val Gly Val Leu Trp Gly Tyr Gly Ser Arg Glu Glu Leu Ala
180 185 190
Phe His Gly Ala Ser Leu Leu Cys Glu Ser Pro Arg Glu Leu Val Glu
195 200 205
Ala Val Ala Gly Leu Ala
210
<210> 106
<211> 223
<212> PRT
<213> Meiothermus timidus
<400> 106
Met Lys Arg Ala Val Leu Phe Asp Val Gly Asp Thr Leu Ile Leu Gly
1 5 10 15
His Pro Lys Leu Trp Leu Trp Pro Leu Leu Gln Glu Arg Gly Leu Ala
20 25 30
Glu Lys Ala Asp Gly Ser Arg Leu Met Glu Ala Val Met Ala Ala Tyr
35 40 45
Arg Val Tyr Asn Glu Arg His Met Gln Ala Thr Thr Ile Glu Ala Ala
50 55 60
Leu Pro Val Trp Ala Glu Phe His Arg Thr Leu Leu Ser Gly Ile Gly
65 70 75 80
Leu Gly Glu His Ala Gly Glu Ile Ser Gly Phe Leu Cys Glu Asn Trp
85 90 95
Arg Asn Pro Lys Val Trp Pro Leu Thr Pro Gly Ala Lys Glu Val Leu
100 105 110
Gly Glu Leu Lys Gly Arg Gly Tyr Lys Leu Gly Val Val Ser Asn Trp
115 120 125
Asp Ala Leu Leu Pro Gly Val Leu Glu Ala Thr Gly Leu Ala Pro Phe
130 135 140
Phe Asp Phe Val Ala Ala Ser Ala Leu Val Gly Ala Ala Lys Pro Asp
145 150 155 160
Pro Arg Ile Phe Arg Val Ala Leu Glu Gly Leu Gly Val Glu Pro His
165 170 175
Gln Ala Leu His Val Gly Asp Ser Pro Asp Asp Leu Gly Gly Ala Ala
180 185 190
Ala Ala Gly Val Glu Ala Leu Leu Phe Asp Pro Tyr Arg Arg Asn Pro
195 200 205
Glu Ala Leu His Glu Leu Ser Gly Val Leu Glu Arg Leu Glu Ala
210 215 220
<210> 107
<211> 198
<212> PRT
<213> Meiothermus timidus
<400> 107
Met Ser Lys Arg Ile Arg Ala Ile Cys Phe Asp Phe Asp Gly Thr Leu
1 5 10 15
Ala Gln Phe Glu Gly Asp Phe Ala Arg Leu Thr Ala Leu Cys Cys Asp
20 25 30
Gln Leu Gly Ile Asp Pro Gly Asp Arg Ala Phe Leu Ala Thr Tyr Glu
35 40 45
Arg Glu Ile Arg Ala Glu Gly His Val Thr Phe Ala Leu Ala Leu Arg
50 55 60
Arg Ala Leu Glu Thr His Gly Tyr Pro Val Pro Ala Gly Leu Glu Ala
65 70 75 80
Phe Ala Ala Glu Val Thr Ala Arg Tyr Ala Ala Glu Val Arg Leu Leu
85 90 95
Pro Gly Val Arg Glu Leu Leu Ala Tyr Phe Ala Pro Leu Pro Lys Ala
100 105 110
Ile Ile Thr Asn Gly Pro Ala Asp Met Gln Trp Ala Ala Ile Arg Ser
115 120 125
Val Gly Leu Glu Arg Glu Phe Glu Ala Ile Val Val Ser Gly Asp Pro
130 135 140
Asp Val Ala Val Arg Lys Pro His Pro Arg Ile Phe His Leu Ala Cys
145 150 155 160
Glu Arg Leu Gly Val Pro Pro His Glu Thr Leu Met Ile Gly Asp His
165 170 175
Leu Glu Ala Asp Ile Gln Gly Ala Ile Ala Ala Gly Leu Gln Gly Leu
180 185 190
His Arg Pro Asn Ser Ala
195
<210> 108
<211> 237
<212> PRT
<213> Meiothermus timidus
<400> 108
Met Ile Pro Glu Glu Leu Ala Leu Leu Ala Arg Gln Pro Leu Leu Val
1 5 10 15
Ile Cys Asp Tyr Asp Gly Thr Leu Ala Pro Ile Ala Ile His Pro Ser
20 25 30
Gln Ala Phe Pro Gln Arg Gly Ala Arg Glu Ala Leu Arg Ala Leu Gln
35 40 45
His His Pro Lys His Gln Ile Met Val Leu Thr Gly Arg Arg Ala Gln
50 55 60
Glu Val Leu Asp Phe Leu Glu Leu Pro Gly Leu Arg Val Ile Gly Leu
65 70 75 80
His Gly Leu Glu Trp Pro Gly Arg Pro Glu Pro Pro Arg Asp Arg Glu
85 90 95
Ala Ile Gln Arg Ile Val Ser Arg Leu Pro Pro Leu Glu Gly Leu Trp
100 105 110
Leu Glu Asp Lys Gly Trp Thr Leu Ala Val His Tyr Arg Asp Ala Pro
115 120 125
Glu Ser Gln Gln Ser Lys Ala Ala Glu Leu Leu Ala Ala Val Pro Leu
130 135 140
Pro Glu Gly Trp Glu Ala Met Pro Gly Lys Lys Val Cys Glu Tyr Arg
145 150 155 160
Pro Ile Gly Tyr Gly Lys Gly Trp Ala Val Glu Gln Leu Met Leu Asp
165 170 175
His Pro Ser His His Pro Val Phe Ile Gly Asp Asp Arg Thr Asp Glu
180 185 190
Glu Gly Phe Ala Ala Val Leu Arg Leu Gly Gly Thr Ala Val Lys Val
195 200 205
Gly Gly Gly Glu Ser Leu Ala Pro Tyr Arg Leu Gly Asn Ser Leu Glu
210 215 220
Val Val Glu Leu Leu Thr Arg Trp Ala Arg Gly Tyr Asp
225 230 235
<210> 109
<211> 249
<212> PRT
<213> Meiothermus timidus
<400> 109
Met Arg Ala Leu Ile Phe Asp Val Asp Gly Val Ile Ala Glu Thr Glu
1 5 10 15
Glu Gly His Arg Leu Ala Phe Asn Arg Ala Phe Ala Glu Ala Gly Leu
20 25 30
Asp Ile Glu Trp Ser Gln Glu Leu Tyr Glu Arg Leu Leu Trp Val Thr
35 40 45
Gly Gly Lys Glu Arg Ile Ala His Tyr Leu Tyr His Cys Pro Glu Cys
50 55 60
Pro Lys Leu Leu Asp Ala Asp Ile Ala Arg Leu His Trp Arg Lys Thr
65 70 75 80
Glu Leu Tyr Asn Gln Ile Val Ala Ala Gly Gly Val Pro Phe Arg Pro
85 90 95
Gly Val Leu Arg Leu Trp Arg Glu Ala Arg Ala Gln Gly Val Lys Leu
100 105 110
Ala Ile Ala Thr Thr Thr Ser Leu Pro Asn Val Glu Val Leu Leu Arg
115 120 125
Gln Ala Gly Glu Glu Val Leu Gly Trp Phe Glu Thr Ile Val Ala Gly
130 135 140
Asp Met Val Pro Arg Lys Lys Pro Ala Pro Asp Val Tyr Leu Lys Val
145 150 155 160
Leu Glu Asn Leu Gly Leu Ala Pro Glu Glu Ala Leu Ala Ile Glu Asp
165 170 175
Ser Gln Asn Gly Leu Ile Ala Ala Gln Lys Ala Gly Ile Pro Thr Leu
180 185 190
Ile Thr Tyr Ser His Tyr Thr Arg Glu Gln Arg Phe Glu Gly Ala Leu
195 200 205
Ala Val Leu Glu His Leu Gly Glu Pro Glu Leu Pro Ala Arg Val Val
210 215 220
Ala Gly Pro Arg Ser Cys Ala Met Val Val Ser Leu Glu Val Leu Arg
225 230 235 240
Glu Trp Tyr Gly Arg Phe Val Arg Ala
245
<210> 110
<211> 157
<212> PRT
<213> Meiothermus timidus
<400> 110
Met Leu Arg Pro Lys Ala Gln Leu Ser Ser Val Leu Glu Ile Thr Pro
1 5 10 15
Gln Trp Leu Lys Thr Arg Gly Ile Lys Gly Leu Leu Leu Asp Leu Asp
20 25 30
Asn Thr Leu Val Pro Tyr Lys Phe Lys Gly Glu Pro Pro Glu Glu Leu
35 40 45
Val Ala Trp Val Lys Ser Leu Glu Ala Ala Gly Ile Arg Val Phe Leu
50 55 60
Val Ser Asn Ala Gln His Lys Arg Leu Arg Ala Trp Ser Ala Lys Leu
65 70 75 80
Gly Val Glu Gly Ile Gly Leu Ala Gly Lys Pro Trp Phe Gly Ile Arg
85 90 95
Lys Gly Leu Lys Arg Leu Gly Leu Ser Pro Ser Gln Val Ala Met Val
100 105 110
Gly Asp Gln Val Phe Thr Asp Val Leu Gly Gly Asn Leu Ala Gly Val
115 120 125
Tyr Thr Ile Leu Val Thr Pro Ile Ser Gln Lys Glu Leu Gly Tyr Thr
130 135 140
Lys Leu Ile Arg Arg Leu Glu Arg Leu Ile Leu Lys Leu
145 150 155
<210> 111
<211> 221
<212> PRT
<213> Meiothermus timidus
<400> 111
Met Lys Ala Leu Val Phe Asp Phe Asp Gly Thr Ile Leu Asp Thr Glu
1 5 10 15
Gln Ala Glu Tyr Thr Ala Trp Thr Gln Ala Tyr Ala Arg Tyr Gly Gly
20 25 30
Glu Leu Thr Leu His Asp Tyr Leu Pro Leu Ile Gly Thr Ala Glu Pro
35 40 45
Val Phe Asp Leu Tyr Ala His Leu Glu Ala Gln Ile Gly Pro Tyr Asp
50 55 60
Arg Glu Ala Phe Asp Arg Glu Arg Gln Glu Val Leu His Ser Leu Leu
65 70 75 80
Ala Gln Leu Glu Pro Leu Pro Gly Val Pro Glu Cys Leu Glu Gln Ala
85 90 95
Arg Lys Leu Gly Leu Lys Leu Ala Val Ala Ser Ser Ser Ser Ala Ala
100 105 110
Trp Val Leu Gly His Leu Glu Gln His His Leu Leu Gln Ala Phe Asp
115 120 125
His Val Ile Thr Arg Glu Tyr Val Ala Arg Thr Lys Pro Asp Pro Ala
130 135 140
Leu Phe Leu Lys Ala Ala Glu Ala Leu Gln Val Ala Pro Ala Glu Cys
145 150 155 160
Val Ala Val Glu Asp Ser Leu Asn Gly Val Arg Ala Ala Arg Ala Ala
165 170 175
Gly Met Phe Thr Ile Ala Val Pro Asn Pro Leu Thr Arg His Gln Asp
180 185 190
Leu Arg Gln Ala Asp Val Leu Leu Pro Ser Leu Ala Gly Leu Glu Leu
195 200 205
Glu Pro Phe Leu Ser Ser Leu Lys Pro Val His Pro Arg
210 215 220
<210> 112
<211> 274
<212> PRT
<213> Meiothermus timidus
<400> 112
Met Gly Gly Arg Cys Arg Gly Pro Val Val Ile Arg Leu Ile Gly Val
1 5 10 15
Asp Leu Asp Trp Thr Phe Val His Pro Ser Gln Gly Val Pro Ala Ser
20 25 30
Ala Trp Gln Ala Val Glu Glu Ala Lys Ala Ala Gly Met Arg Phe Ala
35 40 45
Val Val Thr Gly Arg Pro Phe Gly Gly Tyr Gly Leu Glu Tyr Ala Leu
50 55 60
Lys Met Glu Pro Gln Gly Tyr His Ala Phe Ser Asn Gly Ser Leu Ile
65 70 75 80
Thr Gln Gly Ala Thr Leu Val His Ser Val Ala Met Ser Pro Ser Thr
85 90 95
Tyr Arg Arg Met Val Leu His Ser Arg Ala Gln Gly Leu Pro Phe Tyr
100 105 110
Val Ser Gly Ala Ser Gly Arg Leu Tyr Ser Glu Asn Pro Pro Arg Glu
115 120 125
Leu His Arg Phe Ala Glu Arg Met Gly Val Ser Tyr Gln Arg Ile Asp
130 135 140
Leu Leu Glu Leu Pro Glu Pro Cys Val Gly Gly Val Phe Val Leu Asp
145 150 155 160
Gly Gln Leu Trp Asn Asp Leu Arg Gln Ala Ile Thr Ala Val Glu Gly
165 170 175
Leu Asp Trp Leu Glu Tyr Leu Val Gly Glu Gly Glu Val Val Ala Val
180 185 190
Ala Asp Pro Lys Gly Thr Ser Lys Ala Ser Ala Leu Arg Trp Met Ala
195 200 205
Glu Arg Tyr Gly Leu Ala Met Glu Glu Val Ala Met Ile Gly Asp Ser
210 215 220
Leu Asn Asp Leu Glu Ala Ile Lys Gln Val Gly Leu Gly Ile Ala Met
225 230 235 240
Gly Asn Ala Glu Glu Val Val Arg Glu Ala Ala Ser Val Val Val Ala
245 250 255
Gly Leu Glu Glu Asp Gly Phe Ala Gln Ala Val Arg Leu Cys Leu Ala
260 265 270
Arg Arg
<210> 113
<211> 216
<212> PRT
<213> Meiothermus timidus
<400> 113
Met Arg Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp Thr Asp
1 5 10 15
Arg Leu His Glu Gln Ala Trp Leu Glu Val Leu Leu Pro Tyr Gly Ile
20 25 30
Arg Gly Asp His Ala Phe Tyr Gln Gln His Ile Ser Gly His Leu Asn
35 40 45
Pro Glu Ile Val Ser Arg Leu Leu Pro His Leu Pro Pro Leu Glu Arg
50 55 60
Thr Ala Leu Ile Glu Val Lys Glu Arg Arg Phe Arg Glu Leu Ala Gln
65 70 75 80
Gly Leu Lys Ala Leu Pro Gly Leu Glu Gly Leu Trp Arg Trp Ala Arg
85 90 95
Glu Arg Gly Leu Thr Leu Ala Leu Val Thr Asn Ala Pro Arg Pro Asn
100 105 110
Ala Glu His Val Leu Gln Ala Leu Gly Ala Glu Phe Asp Leu Val Val
115 120 125
Leu Ala Glu Glu Leu Ala Ala Gly Lys Pro Asp Pro Leu Pro Tyr Arg
130 135 140
Thr Ala Leu Gly Arg Leu Gly Leu Asp Pro Ala Glu Ala Leu Ala Phe
145 150 155 160
Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly Ile Arg
165 170 175
Thr Ile Gly Leu Thr Thr Gly His Asp Pro Arg Gly Leu Leu Glu Ala
180 185 190
Gly Ala Phe Leu Leu Ile Asp Asp Phe Ser Asp Gly Arg Leu Trp Glu
195 200 205
Tyr Leu Glu Gly Glu Ser Asp Ser
210 215
<210> 114
<211> 223
<212> PRT
<213> Meiothermus rufus
<400> 114
Met Lys Leu Lys Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp
1 5 10 15
Thr Asp Arg Leu His Glu Gln Ala Trp Leu Glu Gly Leu Ala Arg Tyr
20 25 30
Gly Ile Gln Ala Asp His His Tyr Tyr Gln Thr Gln Ile Ser Gly Gly
35 40 45
Leu Asn Pro Glu Ile Val Ala Arg Leu Leu Pro Gln Leu Ser Pro Ala
50 55 60
Glu Ala Ala Ala Phe Ile Glu Gln Lys Glu Ala Arg Phe Arg Glu Leu
65 70 75 80
Ala Ala Gly Val Thr Pro Leu Pro Gly Leu Gln Glu Leu Trp Ala Trp
85 90 95
Ala Asn Gly His Arg Leu Arg Leu Ala Leu Val Ser Asn Ala Pro Arg
100 105 110
Gln Asn Ala Glu His Met Leu Lys Arg Leu Gly Leu His Phe Asp Cys
115 120 125
Thr Val Leu Ser Glu Glu Leu Pro Ala Gly Lys Pro Asp Pro Leu Pro
130 135 140
Tyr Arg Thr Ala Leu Ala Gln Leu Gly Leu Gly Pro Glu Glu Ala Leu
145 150 155 160
Ala Phe Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly
165 170 175
Leu Arg Thr Val Ala Leu Thr Thr Gly His Pro Ala His Arg Leu Glu
180 185 190
Glu Ala Gly Ala Phe Leu Ala Val Ala Asp Phe Thr Asp Glu Arg Leu
195 200 205
Trp Ala Trp Leu Glu Gln Gln Ser Pro Pro Ser Thr Pro Arg Glu
210 215 220
<210> 115
<211> 218
<212> PRT
<213> Mesotoga infera
<400> 115
Met Ile Lys Ala Ala Ile Phe Asp Met Asp Gly Val Ile Ile Asp Ser
1 5 10 15
Glu Lys Ile Tyr Arg Arg Ala Cys Thr Glu Leu Val Asp Glu Leu Gly
20 25 30
Gly Lys Ile Ser Val Glu Leu Phe Glu Arg Gln Met Gly Leu Lys Met
35 40 45
Ser Glu Thr Gln Lys Val Val Val Gln Thr Ala Gly Leu Glu Ile Glu
50 55 60
Pro Glu Glu Phe Gly Lys Arg Tyr Met Glu Arg Tyr Leu Glu Leu Ala
65 70 75 80
Arg Glu Thr Leu Val Pro Asn Pro Gly Leu Val Asn Leu Leu Asp Phe
85 90 95
Leu Ser Glu Lys Val Glu Leu Ala Ile Ala Ser Ser Thr Glu Lys Ala
100 105 110
Ala Val Glu Glu Leu Met Lys Arg Ile Gly Val Leu Asp Tyr Phe Glu
115 120 125
Ile Ile Val Gly Gly Asp Glu Val Asp Glu Ser Lys Pro Ser Pro Met
130 135 140
Ile Tyr Leu Lys Ala Ser Glu Leu Leu Gly Val Asn Pro Glu Glu Cys
145 150 155 160
Ile Val Ile Glu Asp Ser Pro Asn Gly Ile Lys Ser Gly Ile Gly Ala
165 170 175
Gly Met Glu Val Leu Gly Val Arg His Gly Glu Asn Ala His Leu Asp
180 185 190
Leu Ser Ala Ser Ser His Val Phe Asp Asp Leu Tyr Gly Val Lys Glu
195 200 205
Tyr Leu Glu Thr Val Leu Asn Gly Asn Ala
210 215
<210> 116
<211> 222
<212> PRT
<213> Metallosphaera sedula
<400> 116
Met Trp Ile Val Ala Ser Asp Phe Asp Arg Thr Leu Ser His Glu Asp
1 5 10 15
Asp Ser Phe Val Met Arg Lys Glu Val Ala Ser Lys Ile Asn His Phe
20 25 30
Ser Thr Ile His Arg Phe Phe Val Val Thr Gly Arg Glu Glu Arg Tyr
35 40 45
Met Arg Val Leu Ala Pro Asp Leu Arg Pro Thr Gly Trp Val Leu Glu
50 55 60
Asn Gly Ala Leu Leu Ile Leu Gly Asp Arg Arg Ile Leu Asn Val Pro
65 70 75 80
Glu Asp Trp Phe Glu Thr Arg Lys Ile Ile Gly Glu Lys Leu Thr Lys
85 90 95
Phe Gly Ile Ser Tyr Ser Leu Gly Asp Val Ile Ile Tyr Val Asn Ser
100 105 110
Trp Asn Gly Lys Leu Asp Leu Gly Pro Glu Val Arg Ile Glu Arg Asn
115 120 125
Arg Glu Asp Ala Met Ile Leu Pro Gly Asn Val Asp Lys Gly Thr Gly
130 135 140
Leu Arg Arg Ala Ile Gln Glu Met His Leu Glu Gly Lys Ile Val Ala
145 150 155 160
Val Gly Asp Ala Glu Asn Asp Glu Ser Leu Phe Lys Val Ala Asp Val
165 170 175
Lys Val Ala Val Ala Asn Ala Ile Pro Pro Ile Lys Arg Met Ala Asp
180 185 190
Leu Val Met Glu Lys Glu Asp Gly Glu Gly Val Val Glu Leu Leu Asp
195 200 205
Met Ile Leu Ser Gly Arg Phe Pro Lys Asn Val Asp Val Asn
210 215 220
<210> 117
<211> 224
<212> PRT
<213> Methanocella conradii
<400> 117
Met Ile Lys Ala Val Val Ala Asp Ile Asp Gly Thr Leu Thr Asp Met
1 5 10 15
Gln Arg Arg Ile Ser Val Glu Ala Ile Val Ala Ile Arg Lys Leu Pro
20 25 30
Ile Pro Val Val Leu Ala Ser Gly Asn Val Ile Cys Phe Met Arg Ala
35 40 45
Ala Ser Lys Leu Ile Gly Ala Ser Glu Ala Met Ile Gly Glu Asn Gly
50 55 60
Gly Val Val Gln Val Gly Tyr Asp Ser Pro Pro Val Val Phe Ala Asp
65 70 75 80
Ile Glu Glu Cys Arg Arg Ala Ala Ala Leu Leu Gln Arg Arg Phe Pro
85 90 95
Gly Leu Glu Gln Leu Asp Ala Arg Tyr Arg Leu Ser Glu Leu Ala Phe
100 105 110
Arg Arg Thr Val Asp Ala Asn Glu Leu Arg Ala Ala Leu Asp Met Glu
115 120 125
Tyr Pro Ser Leu Glu Val Val Asp Thr Gln Phe Ala Leu His Leu Lys
130 135 140
His Lys Ser Val Asn Lys Gly Thr Gly Leu Val Asn Val Ala Ser Met
145 150 155 160
Met Gly Phe Lys Pro Glu Glu Phe Ala Ala Leu Gly Asp Ser Ala Asn
165 170 175
Asp Leu Pro Met Phe Glu Ala Ala Gly Leu Gly Leu Ala Val Gly Asn
180 185 190
Ala Val Pro Glu Leu Lys Asp Val Ala Asp Tyr Val Ala Val Lys Pro
195 200 205
Tyr Gly Glu Gly Ala Ala Glu Ala Leu Arg Trp Leu Ala Arg Gln Leu
210 215 220
<210> 118
<211> 237
<212> PRT
<213> Methanocella conradii
<400> 118
Met Ala Glu Arg Cys Ser Val Lys Ile Phe Leu Tyr Arg Ala Val Leu
1 5 10 15
Phe Asp Met Asp Gly Val Ile Ala Asp Thr Met Pro Leu His Tyr Glu
20 25 30
Ala Trp Arg Arg Ala Phe Glu Ala Phe Gly Val His Val Asp Lys Met
35 40 45
Asp Val Tyr Leu Arg Glu Gly Met Thr Thr Met Glu Met Gly Lys Asp
50 55 60
Ile Ala Arg Ser Lys Gly Met Glu Leu Ser Glu Gly Glu Leu Asn Ser
65 70 75 80
Ile Val Glu Leu Lys Thr Arg Ile Phe Asn Glu Leu Val Asp Ser Ser
85 90 95
Val Arg Leu Tyr Glu Gly Val Pro Glu Thr Leu Thr Met Leu Arg Asn
100 105 110
Asn Gly Met Lys Leu Ala Leu Val Thr Gly Ser Arg Arg Thr Ser Ala
115 120 125
Met Ala Val Leu Lys Lys Val Gly Leu Glu Gly Ala Phe Asp Ala Ile
130 135 140
Val Ala Ala Glu Asp Val Arg Arg Gly Lys Pro Asp Ala Glu Pro Tyr
145 150 155 160
Leu Val Ala Met Arg Ala Val Asp Val Pro Ala Leu Asn Cys Val Val
165 170 175
Val Glu Asn Ala Pro Leu Gly Ile Arg Ala Ala Arg Ala Ala Lys Val
180 185 190
Gly Tyr Ile Ile Ala Ile Ala Thr Thr Leu Asp Glu Ala His Leu Lys
195 200 205
Glu Ala Asp Glu Val Ala Pro Ser Phe Pro Glu Leu Glu Gln Cys Ile
210 215 220
Ala Arg Arg Phe Glu Ala Arg Pro Gly Arg Ala Ile Met
225 230 235
<210> 119
<211> 209
<212> PRT
<213> Methanocella conradii
<400> 119
Met Pro Arg Ile Arg Gly Leu Ile Phe Asp Leu Asp Gly Thr Leu Ile
1 5 10 15
Asp Asn Tyr Asp Gly Tyr Met Glu Leu Met Leu Arg Arg Val Gly Arg
20 25 30
Asp Leu Gly Arg Glu Phe Thr Leu Ser His Ala Lys Glu Leu Trp Tyr
35 40 45
Ser Ile Asn Ser Glu Ser Arg Asp Glu Val Ile Met Arg Trp Gly Val
50 55 60
Asn Pro Asp Arg Phe Trp Thr Val Phe Asn Arg Tyr Glu Asp Ile Asn
65 70 75 80
Glu Lys Leu Glu Asn Thr Tyr Leu His Asp Asp Ala Asp Ile Leu Lys
85 90 95
Gly Leu Asn Ile Pro Lys Gly Ile Val Thr His Thr Ser Tyr Glu His
100 105 110
Thr Asp Arg Leu Leu Gln Lys Val Gly Met Arg Gln Tyr Phe Arg Pro
115 120 125
Ile Ile Ala Cys Thr Glu Asp Thr Gly Tyr Lys Pro Ser Pro Leu Pro
130 135 140
Ile Ile Tyr Cys Val Val Gly Met Lys Leu Lys Pro Glu Glu Val Ala
145 150 155 160
Tyr Val Gly Asp Thr Leu Ser Asp Met Leu Ala Ala Arg Tyr Ala Gly
165 170 175
Val Lys Ser Ile Tyr Ile Asn Arg Tyr Asn Arg Pro Ile Lys Ala Ser
180 185 190
Pro Asp Tyr Glu Ile Lys Arg Met Asp Glu Leu Leu Glu Ile Leu Gly
195 200 205
Gln
<210> 120
<211> 216
<212> PRT
<213> Methanococcoides methylutens
<400> 120
Met Leu Asn Ser Leu Ile Phe Asp Ala Asp Gly Val Leu Met Asp Ser
1 5 10 15
Met Pro Cys His Ala Asp Ala Trp Val His Thr Phe Ser Glu Val Gly
20 25 30
Ile Asp Ile Val Arg Gln Asp Ile Tyr Asp Ile Glu Gly Ser Asn His
35 40 45
Val Gly Val Ile Lys Leu Ile Phe Lys Lys Ala Gly Arg Glu Ala Asp
50 55 60
Pro Glu Ile Ile Glu Lys Leu Arg Val Arg Lys Arg Glu Leu Phe Leu
65 70 75 80
Lys Lys Lys Asn Ile Asp Pro Phe Asp Gly Met Tyr Asn Leu Leu Lys
85 90 95
Lys Leu Lys Asn Asp Phe His Leu Ala Val Val Ser Gly Ser Asp Arg
100 105 110
Pro Ile Val Asp Ser Met Ile Asn Glu Phe Tyr Pro Asp Ile Phe Glu
115 120 125
Val Thr Ile Ser Gly Ala Asp Val Asn Asn Gly Lys Pro Asp Pro Glu
130 135 140
Pro Tyr Leu Lys Ala Met Glu Ile Leu Lys Val Lys Lys Glu Asn Cys
145 150 155 160
Leu Val Ile Glu Asn Ala Pro Leu Gly Val Asp Ser Ala Lys Asn Ala
165 170 175
Gly Ile Tyr Cys Val Ala Val Pro Thr Tyr Val Asp Ala Glu Lys Leu
180 185 190
Lys Arg Ala Asp Gln Ile Ile Gln Asp His Lys Gln Leu Ile Glu Phe
195 200 205
Leu Ser Asp Ile Glu Ser Pro Arg
210 215
<210> 121
<211> 227
<212> PRT
<213> Methanococcoides methylutens
<400> 121
Met Val Phe Lys Ala Leu Val Val Asp Ile Asp Gly Thr Ile Thr Asn
1 5 10 15
Pro Asp Arg Ser Leu Asp Leu Arg Val Ala Lys Arg Phe Arg Glu Leu
20 25 30
Arg Val Pro Val Val Leu Ser Thr Gly Asn Pro Leu Cys Tyr Val His
35 40 45
Ala Ala Ala Lys Leu Ile Gly Leu Gly Gly Ile Val Ile Ala Glu Asn
50 55 60
Gly Gly Val Ile Ser Thr Gly Phe Asp Lys Pro Ser Ile Ile Ala Asp
65 70 75 80
Gly Met Glu Lys Cys Glu Glu Ala Tyr Glu Leu Leu Ser Asn Tyr Phe
85 90 95
Asp Leu Glu Lys Leu Asp Ala Ala Tyr Arg Lys Thr Glu Val Val Leu
100 105 110
Arg Arg Gly Met Asp Ile Ser Gln Leu Arg Glu Ile Val Glu Asp Asn
115 120 125
Gly Met Glu Val Glu Ile Ile Asp Thr Gly Tyr Ala Val His Ile Lys
130 135 140
Asp Gly Lys Ile Asn Lys Gly Thr Gly Leu His Thr Val Ala Glu Leu
145 150 155 160
Met Gly Ile Asp Thr Lys Asp Phe Leu Ala Ile Gly Asp Ser Cys Asn
165 170 175
Asp Ala Glu Met Met Arg Glu Ala Gly Leu Gly Ile Ala Val Gly Asn
180 185 190
Ala Asp Asp Asp Ala Arg Lys Ala Ala Ser Met Val Thr Lys Ala Ser
195 200 205
Phe Gly Glu Gly Met Leu Glu Ala Ile Glu Tyr Ala Phe Ser Asn Gly
210 215 220
Leu Leu Glu
225
<210> 122
<211> 234
<212> PRT
<213> Methanohalobium evestigatum
<400> 122
Met Lys Tyr Lys Ala Leu Val Ala Asp Ile Asp Gly Thr Ile Thr His
1 5 10 15
Gln Asn Arg Arg Leu Asn Leu Lys Ala Ala Glu Met Leu Tyr Arg Leu
20 25 30
Asn Asp Glu Ser Gly Ile Pro Val Val Leu Ala Thr Gly Asn Ile Leu
35 40 45
Cys Phe Ser His Ala Ala Ser Lys Leu Ile Gly Leu Ser Gly Tyr Val
50 55 60
Ile Ala Glu Asn Gly Gly Val Val Ser Glu Gly Phe Asp Thr Asn Pro
65 70 75 80
Tyr Ile Met Gly Asp Ile Lys Glu Cys Arg Lys Ala Phe Ser Phe Leu
85 90 95
Ser Glu Tyr Ile Pro Leu Thr Lys Leu Asp Pro Glu Tyr Arg Lys Thr
100 105 110
Glu Ile Ala Leu Arg Arg Asn Phe Asp Leu Glu Tyr Ala Lys Asn Val
115 120 125
Ile Lys Ser Gly Lys Tyr Asn Val Asp Leu Ile Asp Thr Gly Tyr Ala
130 135 140
Val His Ile Lys Ser Asn Thr Met Asn Lys Gly Thr Gly Leu Val Lys
145 150 155 160
Ile Ala Glu Leu Met Gly Leu Asn Pro Lys Glu Phe Val Ala Ile Gly
165 170 175
Asp Ser Glu Asn Asp Leu Glu Met Ile Glu Phe Ala Gly Ile Gly Ile
180 185 190
Ala Val Ala Asn Ser Asp Gln Lys Thr Arg Ser Ile Ala Asp Ile Val
195 200 205
Thr Asp Ala Ser Tyr Gly Glu Gly Val Val Glu Ala Ile Glu Arg Leu
210 215 220
Tyr Ser Cys Asp Trp Asn Asp Thr Cys Leu
225 230
<210> 123
<211> 217
<212> PRT
<213> Methanohalobium evestigatum
<400> 123
Met Leu Lys Ala Ile Ile Phe Asp Phe Asp Gly Val Leu Ala Asp Ser
1 5 10 15
Met Pro Tyr His Ala Gln Ala Trp Lys Ile Ala Phe Glu Lys Ala Gly
20 25 30
Ile Glu Ile Asn Lys Asp Asp Ile Tyr Glu Ile Glu Gly Ser Asn His
35 40 45
Val Gly Ile Ile Gln Leu Met Phe Gln Lys Met Gly Lys Ser Pro Gln
50 55 60
Pro His Gln Phe Asp Glu Ile Ala Gln Glu Lys Arg Lys Ile Phe Ser
65 70 75 80
Gln Ile Ala Asn Leu Lys Asn Phe Glu Asp Met Asp Lys Cys Leu Ser
85 90 95
Gln Leu Lys Asn Lys Phe Lys Leu Ala Val Val Ser Gly Ala Asp Arg
100 105 110
Asn Ala Val Phe Asn Met Val Gly Arg Phe Tyr Pro Asp Val Phe Asp
115 120 125
Val Ile Val Thr Gly Glu Asp Val Asp Asn Gly Lys Pro Ser Pro Glu
130 135 140
Pro Tyr Leu Lys Ala Leu Gly Met Leu Asn Val Glu Lys Asn Glu Cys
145 150 155 160
Leu Val Val Glu Asn Ala Pro Leu Gly Val Asp Ser Ala Lys Asn Ala
165 170 175
Gly Leu Tyr Cys Val Ala Val Pro Thr Tyr Val Asp Pro Glu Lys Leu
180 185 190
Asp Lys Ala Asp Val Val Val Glu Asp His Ser Asp Leu Lys Lys Tyr
195 200 205
Leu Gln Lys Ile Tyr Ser Glu Gln Ala
210 215
<210> 124
<211> 226
<212> PRT
<213> Methanolobus tindarius
<400> 124
Met Lys Phe Lys Ala Ile Val Ile Asp Ile Asp Gly Thr Ile Thr His
1 5 10 15
Arg Asp Arg Lys Leu Ser Leu Thr Ala Ala Ala Lys Leu Arg Ala Leu
20 25 30
Asp Ile Pro Val Val Ile Ala Thr Gly Asn Val Leu Cys Tyr Ala Lys
35 40 45
Ala Thr Ala Lys Leu Val Gly Val Cys Cys Asn Ile Ile Ala Glu Asn
50 55 60
Gly Gly Val Ile Ile Asp Gly Phe Asp Asn Asp Pro Ile Val Ser Asp
65 70 75 80
Val Ile His Glu Cys Glu Glu Ala Tyr Glu Phe Leu Ser Ser Glu Phe
85 90 95
Asn Met Glu Lys Leu Asp Ser Val His Arg Arg Thr Glu Ile Val Leu
100 105 110
Arg Ser Asn Phe Asp Val Glu Lys Ala Arg Glu Val Leu Gly Thr His
115 120 125
Phe Pro Asn Val Glu Ile Ile Asp Thr His Phe Ala Ile His Ile Lys
130 135 140
Ser Lys Lys Ile Asn Lys Gly Thr Gly Leu Phe Lys Met Ala Glu Met
145 150 155 160
Met Gly Leu Glu Thr Thr Asp Phe Val Ala Ile Gly Asp Ser Ile Asn
165 170 175
Asp Leu Glu Met Leu Gln Glu Ala Gly Phe Ala Val Ala Val Gly Asn
180 185 190
Ala Asp Asp Phe Leu Lys Asp Ile Ala Asp Tyr Val Ser Glu Ala Ser
195 200 205
Tyr Gly Asp Gly Thr Val Glu Ala Ile Asp Tyr Leu Ile Ser Ser Gly
210 215 220
Leu Ile
225
<210> 125
<211> 217
<212> PRT
<213> Methanolobus tindarius
<400> 125
Met Ile Lys Gly Ile Ile Phe Asp Ser Asp Gly Val Leu Val Asn Ser
1 5 10 15
Met Pro Phe His Ala Lys Ala Trp Val Glu Val Phe Ala Glu Tyr Gly
20 25 30
Ile Glu Val Thr Glu Glu Asp Ile Tyr Glu Ile Glu Gly Ser Asn His
35 40 45
Val Gly Val Ile Asn Ile Phe Phe Gly Lys Ala Gly Arg Thr Pro Glu
50 55 60
Pro Glu Ile Tyr Ala Glu Ile Leu Glu Lys Lys Arg Ala His Phe Leu
65 70 75 80
Glu Asn Asn Arg Ala Glu Val Phe Glu Gly Met Tyr Asp Cys Leu Ser
85 90 95
Ser Leu Lys Asn Lys Phe Lys Leu Ala Val Ala Ser Gly Ala Asp Arg
100 105 110
Thr Ile Val Thr Ser Leu Met Asp Lys Phe Tyr Pro Gly Val Phe Asp
115 120 125
Ala Ile Ile Ser Gly Glu Asp Val Glu Asn Gly Lys Pro Asp Pro Glu
130 135 140
Pro Tyr Glu Lys Ala Ile Val Lys Leu Gly Leu Ser Lys Asp Glu Cys
145 150 155 160
Leu Val Val Glu Asn Ala Pro Leu Gly Val Glu Ser Ala Lys Asn Ala
165 170 175
Gly Val Phe Cys Val Gly Val Pro Thr Tyr Leu Asp Glu Ser Lys Leu
180 185 190
Lys Glu Ala Asp Phe Val Val Arg Asn His Ser Glu Leu Ile Glu Tyr
195 200 205
Leu Thr Asp Leu Lys Asp Ser Ser Leu
210 215
<210> 126
<211> 228
<212> PRT
<213> Methanolobus tindarius
<400> 126
Met Leu Met Lys Phe Lys Ala Ile Val Ile Asp Ile Asp Gly Thr Ile
1 5 10 15
Thr His Arg Asp Arg Lys Leu Ser Leu Thr Ala Ala Ala Lys Leu Arg
20 25 30
Ala Leu Asp Ile Pro Val Val Ile Ala Thr Gly Asn Val Leu Cys Tyr
35 40 45
Ala Lys Ala Thr Ala Lys Leu Val Gly Val Cys Cys Asn Ile Ile Ala
50 55 60
Glu Asn Gly Gly Val Ile Ile Asp Gly Phe Asp Asn Asp Pro Ile Val
65 70 75 80
Ser Asp Val Ile His Glu Cys Glu Glu Ala Tyr Glu Phe Leu Ser Ser
85 90 95
Glu Phe Asn Met Glu Lys Leu Asp Ser Val His Arg Arg Thr Glu Ile
100 105 110
Val Leu Arg Ser Asn Phe Asp Val Glu Lys Ala Arg Glu Val Leu Gly
115 120 125
Thr His Phe Pro Asn Val Glu Ile Ile Asp Thr His Phe Ala Ile His
130 135 140
Ile Lys Ser Lys Lys Ile Asn Lys Gly Thr Gly Leu Phe Lys Met Ala
145 150 155 160
Glu Met Met Gly Leu Glu Thr Thr Asp Phe Val Ala Ile Gly Asp Ser
165 170 175
Ile Asn Asp Leu Glu Met Leu Gln Glu Ala Gly Phe Ala Val Ala Val
180 185 190
Gly Asn Ala Asp Asp Phe Leu Lys Asp Ile Ala Asp Tyr Val Ser Glu
195 200 205
Ala Ser Tyr Gly Asp Gly Thr Val Glu Ala Ile Asp Tyr Leu Ile Ser
210 215 220
Ser Gly Leu Ile
225
<210> 127
<211> 218
<212> PRT
<213> Methanosarcina siciliae
<400> 127
Met Leu Lys Ala Ile Ile Phe Asp Val Asp Gly Val Leu Val Asp Ser
1 5 10 15
Met Arg Phe Gln Ala Glu Ala Trp Val Lys Thr Leu Lys Glu Val Gly
20 25 30
Val Thr Val Ser Arg Glu Asp Ile Tyr Glu Leu Glu Gly Ser Asn Asn
35 40 45
Leu Gly Leu Ile Lys Leu Val Phe Glu Lys Ala Gly Lys Glu Pro Glu
50 55 60
Pro Trp His Tyr Glu Gln Leu Pro Val Lys Lys Arg Glu Val Leu Glu
65 70 75 80
Phe Asp Arg Ile Lys Pro Phe Glu Gly Met Pro Ser Cys Leu Asn Gln
85 90 95
Leu Lys Arg His Phe Arg Leu Ala Met Val Ser Gly Ser Asn Phe Asp
100 105 110
Ile Val Ser Thr Phe Ala Asn Ser Phe Phe Pro Asp Cys Phe Glu Val
115 120 125
Ile Val Thr Gly Gly Asp Leu Glu Arg Gly Lys Pro Cys Pro Asp Pro
130 135 140
Tyr Ile Lys Ala Leu Glu Lys Leu Asp Leu Ala Thr Asn Glu Cys Ile
145 150 155 160
Val Val Glu Asn Ala Pro Leu Gly Ile Thr Ala Ala Lys Arg Ala Gly
165 170 175
Leu Tyr Cys Val Ala Val Ala Ser Met Leu Ser Pro Glu Lys Val Lys
180 185 190
His Ala Asp Ile Val Phe Glu Asn His Ala Val Leu Leu Asp Tyr Leu
195 200 205
Lys Ser Leu Ile Pro Gly Glu Ser His Thr
210 215
<210> 128
<211> 253
<212> PRT
<213> Methanosarcina siciliae
<400> 128
Met Ser Ser Ile Ile Asn Tyr Gly Ser Ile Ile Asn Tyr Glu Trp Gly
1 5 10 15
Ser Ile Leu Ser Pro Leu Ser His Leu Phe Ser Met Lys Phe Lys Ala
20 25 30
Ile Val Val Asp Ile Asp Gly Thr Ile Thr Cys Glu Asn Arg Glu Leu
35 40 45
His Leu Gly Ala Val Lys Lys Ile Arg Ser Leu Lys Val Pro Val Val
50 55 60
Leu Ala Thr Gly Asn Ile Ile Cys Tyr Ala Arg Thr Ala Ser Lys Leu
65 70 75 80
Ile Gly Leu Glu Gly Ala Val Val Ala Glu Asn Gly Gly Ala Val Thr
85 90 95
Val Arg Tyr Asp Leu Asn Gly Thr Phe Glu Glu Ser Leu Glu Glu Cys
100 105 110
Glu Lys Ala Phe Ser Phe Leu Ser Glu His Phe Gln Leu Thr Lys Leu
115 120 125
Asp Pro Phe Tyr Arg Lys Thr Glu Ile Ala Leu Arg Arg Asp Phe Asp
130 135 140
Leu Glu Lys Ala Arg Ala Leu Leu Lys Thr Gln Leu Leu Asp Val Glu
145 150 155 160
Met Val Asp Thr Lys Tyr Ala Val His Ile Lys Ser Thr Arg Ile Asn
165 170 175
Lys Gly Ser Gly Leu Arg Lys Leu Ala Glu Met Met Gly Leu Glu Ala
180 185 190
Lys Asp Phe Val Ala Ile Gly Asp Ser Glu Asn Asp Val Glu Met Phe
195 200 205
Lys Ala Ala Gly Phe Gly Ile Ala Val Ala Asn Gly Asp Thr Lys Ile
210 215 220
Lys Glu Ala Ala Asp Tyr Val Thr Glu Ala Ser Phe Gly Lys Gly Ala
225 230 235 240
Val Glu Ala Phe Glu Tyr Leu Glu Ser Lys Gly Leu Ile
245 250
<210> 129
<211> 213
<212> PRT
<213> Methanosarcina siciliae
<400> 129
Met Lys Tyr Asn Thr Val Ile Phe Asp Phe Asp Tyr Thr Leu Ala Asp
1 5 10 15
Ala Thr Asp Gly Ile Val Ser Ser Phe Asn His Ala Phe Ser Glu Leu
20 25 30
Asp Leu Ala Ser Tyr Asp Ile Glu Ser Ile Lys Arg Thr Val Gly Leu
35 40 45
Ser Leu Asp Glu Ala Phe Val Gln Leu Thr Ala Asn Glu Asp Lys Ala
50 55 60
Leu Arg Asn Arg Phe Lys Ile Leu Phe Lys Glu Lys Ala Asp Glu Val
65 70 75 80
Met Ser Lys Asn Thr Val Leu Phe Asp Asp Thr Ile Ser Thr Leu Arg
85 90 95
Gln Leu Lys Arg Asp Gly Leu Asn Thr Gly Ile Val Thr Thr Lys Tyr
100 105 110
His Tyr Arg Ile Met Glu Thr Leu Asn Thr Tyr Gly Ile Ser Asp Leu
115 120 125
Val Asp Ile Ile Val Gly Gly Glu Asp Val Lys Ala Pro Lys Pro Ser
130 135 140
Pro Glu Gly Leu Leu Leu Ala Ile Asp Ser Leu Asn Ser Arg Gln Asn
145 150 155 160
Asn Val Leu Tyr Ile Gly Asp Ser Leu Val Asp Ala Lys Thr Ala Leu
165 170 175
Ala Ala Asn Val Asp Phe Val Ala Val Thr Thr Gly Thr Thr Glu Val
180 185 190
Lys Glu Phe Ser Gln Tyr Pro Cys Ile Arg Ile Leu Lys Asn Leu Ser
195 200 205
Glu Leu Phe Asp Arg
210
<210> 130
<211> 224
<212> PRT
<213> Methanothermus fervidus
<400> 130
Met Lys Ile Glu Ala Ile Ala Val Asp Ile Asp Gly Thr Ile Thr Asp
1 5 10 15
Thr Lys Arg Arg Leu Cys Ile Asp Ala Ile Lys Ala Leu Arg Asp Ala
20 25 30
Glu Lys Leu Gly Ile Lys Val Thr Leu Val Thr Gly Asn Ile Leu Cys
35 40 45
Tyr Ala Tyr Ala Thr Ser Thr Leu Ile Gly Leu Ser Gly Gly Val Val
50 55 60
Ala Glu Asn Gly Gly Val Ile Tyr Val Asn Asp Glu Val Asn Val Leu
65 70 75 80
Gly Asp Ile Lys Lys Ala Lys Lys Ala Tyr Asn Tyr Leu Lys Ser Ile
85 90 95
Tyr Pro Val Glu Lys Val Gln Phe Ser Glu Leu Arg Val Ser Glu Ile
100 105 110
Ala Ile Lys Arg Thr Ile Pro Val Glu Ile Ile Lys Asp Ala Leu Lys
115 120 125
Asp Phe Asp Val Glu Val Tyr Asp Thr Lys Phe Ala Ile His Leu Thr
130 135 140
Asp Pro Lys Val Asp Lys Gly Ser Ser Leu Lys Ile Leu Thr Lys Lys
145 150 155 160
Leu Gly Ile Asp Leu Glu Lys Thr Ile Ala Ile Gly Asp Ser Glu Asn
165 170 175
Asp Leu Glu Phe Leu Asp Val Ala Gly Val Lys Val Ala Val Ala Asn
180 185 190
Ala Asn Lys Glu Leu Lys Glu Ile Ala Asp Tyr Val Thr Lys Lys Pro
195 200 205
Tyr Gly Ser Gly Val Ala Glu Ala Ile Lys Lys Tyr Val Thr Leu Ser
210 215 220
<210> 131
<211> 277
<212> PRT
<213> Petrotoga mobilis
<400> 131
Met Lys Lys Thr Phe Val Phe Asp Leu Asp Gly Thr Leu Leu Asn Ser
1 5 10 15
Glu Val Arg Ile Ser Pro Lys Thr Tyr Gln Ala Leu Lys Arg Leu Lys
20 25 30
Glu Glu Gly His Ile Ile Ile Ile Ala Ser Gly Arg Met Tyr Ala Ser
35 40 45
Thr Met Tyr Val Val Glu Asn Phe Leu Pro Phe Leu Lys Gly Asn Val
50 55 60
Ile Ile Ser Ser Tyr Asn Gly Gly Tyr Ile Val Asp His Asn Gly Lys
65 70 75 80
Val Val Phe Glu Lys Gly Val Ala Asn Glu Ser Ala Ile Lys Cys Ile
85 90 95
Lys Phe Leu Arg Asp Leu Asn Ile His Arg His Ile Tyr Ile Asn Asp
100 105 110
Lys Leu Ile Ser Glu Ile Asp Asp Lys Glu Ile Arg Asp Tyr Ser Lys
115 120 125
His Ser Phe Val Asp Tyr Val Leu Val Asp Asp Leu Ile Ala Glu Ile
130 135 140
Glu Thr Ser Ser His Pro Thr Leu Lys Ile Leu Ala Ile Gly Glu Pro
145 150 155 160
Asp Lys Ile Asp Val Ile Lys Lys Leu Ala Glu Asn Glu Leu Gln Gly
165 170 175
Glu Phe Asn Leu Met Lys Ser Trp Asp Thr Tyr Leu Asp Phe Ile Pro
180 185 190
Tyr Gly Val Ser Lys Gly Asn Ser Leu Lys Ile Ile Ser Lys Ile Tyr
195 200 205
Asn Leu Asp Pro Asn Thr Leu Tyr Val Phe Gly Asp Ser Glu Asn Asp
210 215 220
Ile Asp Met Leu Glu Leu Thr Lys Asn Ser Phe Ala Met Gly Asn Ala
225 230 235 240
Lys Glu Asp Val Lys Lys Val Ala Asn Tyr Leu Leu Pro Ser Asn Asp
245 250 255
Glu Asp Gly Val Ala Tyr Ala Ile Glu Lys Ile Leu Ala Asp Asp Leu
260 265 270
Asp Asn Val Asn Ile
275
<210> 132
<211> 214
<212> PRT
<213> Petrotoga mobilis
<400> 132
Met Ile Lys Ala Cys Ile Phe Asp Leu Asp Gly Val Ile Val Asp Thr
1 5 10 15
Ala Lys Tyr His Tyr Met Ala Trp Lys Arg Leu Ala Asp Glu Leu Asn
20 25 30
Ile Pro Phe Asn Glu Lys Asp Asn Glu Arg Leu Lys Gly Val Ser Arg
35 40 45
Met Lys Ser Leu Glu Ile Ile Leu Asp Leu Gly Asn Val Asn Leu Ser
50 55 60
Gln Glu Glu Lys Glu Glu Leu Ala Gln Lys Lys Asn Asn Trp Tyr Val
65 70 75 80
Glu Cys Ile Ser Asn Met Asp Lys Ser Glu Leu Leu Pro Gly Val Glu
85 90 95
Glu Phe Ile Lys Asn Leu Lys Arg Lys Gly Ile Lys Val Ala Ile Ala
100 105 110
Ser Ala Ser Lys Asn Thr Lys Leu Ile Leu Lys Lys Leu Asn Leu Glu
115 120 125
Asp Thr Phe Asp Ala Val Ile Asp Gly Thr Met Ile Asp Lys Ala Lys
130 135 140
Pro Asn Pro Glu Ile Phe Leu Lys Ala Ser Asp Tyr Leu Asn Val Lys
145 150 155 160
Pro Glu Glu Cys Leu Val Phe Glu Asp Ala Val Ala Gly Val Gln Ala
165 170 175
Ala Lys Lys Ala Gly Met Lys Val Ile Gly Val Gly Glu Lys Lys Val
180 185 190
Leu Lys Gly Ala Asp Lys Val Ile Lys Asn Phe Glu Asn Val Asp Leu
195 200 205
Thr Leu Ile Glu Gly Ile
210
<210> 133
<211> 236
<212> PRT
<213> Picrophilus torridus
<400> 133
Met Phe Met Thr Lys Asp Leu Phe Phe Val Tyr Ile Phe Thr Thr Met
1 5 10 15
Ile Lys Leu Val Val Leu Asp Val Asp Gly Thr Leu Thr Asp Lys Ser
20 25 30
Arg Met Ile Ser Val Asn Ala Val Asn Ala Ile Arg Asn Leu Lys Thr
35 40 45
Lys Val Ala Leu Val Ser Gly Asn Val Leu Pro Val Leu Tyr Gly Leu
50 55 60
Lys Ile Tyr Ile Gly Phe Asp Gly Tyr Ile Phe Ala Glu Asn Gly Gly
65 70 75 80
Ile Ala Leu Ile Asn Asn Asn Ile Glu Lys Phe Phe Glu Lys Asp Gly
85 90 95
Pro Glu Ser Phe Leu Asn Asp Ile Ser Gly Tyr Thr Ser Ala Arg Gly
100 105 110
Ile Leu Thr Asn Arg Trp Arg Glu Thr Ser Met Ala Phe Thr Ala Asn
115 120 125
His Asp Glu Met Asp Ile Ile Asp Arg Glu Ala Ala Ser Arg Asp Leu
130 135 140
Tyr Ile Val Asp Ser Gly Phe Thr Leu His Ile Leu Asn Lys Gly Gln
145 150 155 160
Asp Lys Gly Phe Ala Val Lys Lys Met Ile Asp Ile Met Asn Ile Asp
165 170 175
Tyr Asn Asn Val Leu Val Ile Gly Asp Ser Gln Asn Asp Glu Ser Met
180 185 190
Phe Ser Leu Gly Thr Leu Ser Ala Cys Pro Gly Asn Ala Ser Glu Lys
195 200 205
Ile Lys Glu Met Ser Asn Tyr Val Ser Gly Lys Cys Tyr Gly Asp Glu
210 215 220
Leu Phe Asp Val Phe Arg His Phe Asp Leu Ile His
225 230 235
<210> 134
<211> 202
<212> PRT
<213> Picrophilus torridus
<400> 134
Met Phe Lys Ala Val Ile Phe Asp Leu Asp Gly Thr Ile Leu Asp Ser
1 5 10 15
Ile Pro Leu Arg Val Arg Ser Trp Gln Gln Ala Phe Asp Asp Tyr Asn
20 25 30
Ile Ser Ala Asp Pro Glu Ile Ile Arg Leu Met Ile Gly Tyr Pro Gly
35 40 45
Ser Met Leu Ile Lys Lys Val Asn Ala Met Asn Pro Glu Ile Glu Phe
50 55 60
Arg Glu Glu Tyr Tyr Phe Lys Met His Leu Asp Glu Leu Arg Phe Phe
65 70 75 80
Pro Asp Val Asp Asp Thr Phe Lys Glu Leu Arg Lys Leu Gly Val Lys
85 90 95
Ile Ala Val Val Thr Ser Ser Arg Lys Asp Phe Val Met Arg Leu Asn
100 105 110
Ile Lys Ala Asp Ala Ile Val Thr Ile Asp Asp Val Lys Asn Gly Lys
115 120 125
Pro Asp Thr Glu Pro Tyr Ile Lys Ala Met Ser Met Met Asn Val Lys
130 135 140
Pro Asp Glu Thr Val Val Val Gly Asp Ile Asp Asn Asp Leu Ile Pro
145 150 155 160
Ser Arg Glu Leu Gly Cys Leu Ser Val Leu Val Arg His Asn Arg Asn
165 170 175
Val Ser Ser Asp His Ala Asp Ile Ile Ile Glu Glu Ile Lys Glu Val
180 185 190
Leu Asn Ile Phe Asn Asp Lys Lys Asp Leu
195 200
<210> 135
<211> 228
<212> PRT
<213> Pseudonocardia thermophila
<400> 135
Met Thr Asp Arg Ala Asp Thr Ala His Pro Asp Thr Ala His Pro Asp
1 5 10 15
Thr Ala Ile Val Asp Val Asp Gly Thr Leu Val Asp Thr Asn Tyr His
20 25 30
His Ala Leu Ala Trp Phe Arg Ala Phe Arg Arg Phe Gly Val Thr Leu
35 40 45
Pro Val Trp Arg Leu His Arg Ala Ile Gly Met Gly Gly Asp Gln Leu
50 55 60
Val Pro Ala Val Ala Gly Asn Arg Phe Glu Asp Asp His Gly Asp Ala
65 70 75 80
Val Arg Glu Ala Trp Lys Asp Glu Phe Asp Pro Leu Met Gly Glu Val
85 90 95
Arg Pro Phe Gly Gly Val Cys Glu Leu Leu Thr Ala Phe Gly Glu Ser
100 105 110
Gly Leu Lys Val Val Leu Ala Ser Ser Gly Ala Pro Glu His Val Asp
115 120 125
Ala Tyr Leu Asp Leu Phe Asp Gly Arg Arg Leu Ala Asp Ala Trp Thr
130 135 140
Thr Ser Glu Asp Val Asp Arg Thr Lys Pro Glu Pro Asp Leu Ile Thr
145 150 155 160
Ala Ala Leu Asp Arg Val Arg Gly Glu Val Gly Ile Val Ile Gly Asp
165 170 175
Ser Val Trp Asp Phe Arg Ala Ala Glu Arg Ala Gly Gln Arg Gly Tyr
180 185 190
Ala Ile Arg Thr Gly Gly Phe Ser Glu Ser Glu Leu Arg Glu Ala Gly
195 200 205
Ala Arg Asn Val Phe Glu Ser Leu Pro Glu Leu His Gln Ala Leu Pro
210 215 220
Arg Ile Leu Gly
225
<210> 136
<211> 273
<212> PRT
<213> Pyrococcus furiosus
<400> 136
Met Glu Met Arg Ile Val Phe Asp Met Asp Gly Val Leu Tyr Arg Gly
1 5 10 15
Asn Thr Pro Ile Glu Gly Ala Arg Glu Val Ile Glu Phe Leu Lys Glu
20 25 30
Lys Gly Ile Lys Phe Ala Phe Leu Thr Asn Asn Ser Thr Lys Thr Pro
35 40 45
Glu Met Tyr Arg Glu Arg Leu Leu Lys Met Gly Ile Asp Val Pro Ala
50 55 60
Asp Ser Ile Ile Thr Ser Gly Leu Ala Thr Arg Ile Tyr Met Lys Lys
65 70 75 80
His Phe Glu Pro Gly Lys Ile Phe Val Ile Gly Gly Arg Gly Leu Val
85 90 95
Glu Glu Met Glu Lys Leu Gly Trp Gly Ile Val Ser Val Glu Glu Ala
100 105 110
Arg Glu Gly Ile Trp Lys Glu Val Lys Tyr Val Val Val Gly Leu Asp
115 120 125
Pro Glu Leu Thr Tyr Glu Lys Leu Lys Tyr Gly Thr Leu Ala Ile Arg
130 135 140
Asn Gly Ala Glu Phe Ile Gly Thr Asn Pro Asp Arg Thr Tyr Pro Gly
145 150 155 160
Glu Glu Gly Ile Tyr Pro Gly Ala Gly Ser Ile Ile Ala Ala Leu Glu
165 170 175
Ala Ala Thr Asp Lys Lys Pro Leu Ile Ile Gly Lys Pro Asn Glu Pro
180 185 190
Met Tyr Glu Val Leu Arg Glu Lys Leu Gly Glu Gly Glu Val Trp Met
195 200 205
Val Gly Asp Arg Leu Asp Thr Asp Ile Leu Phe Ala Lys Lys Phe Gly
210 215 220
Met Lys Ala Ile Met Val Leu Thr Gly Val His Ser Leu Ser Asp Ile
225 230 235 240
Glu Lys Ser Asp Ile Lys Pro Asp Leu Val Leu Pro Ser Ile Lys Glu
245 250 255
Leu Leu Glu Tyr Leu Lys Ile Leu His Asp Glu Asp Lys Glu Gly Thr
260 265 270
Lys
<210> 137
<211> 351
<212> PRT
<213> Pyrococcus furiosus
<400> 137
Met Ser Lys Pro Leu Phe Val Thr Pro Pro Tyr Val Ser Thr Leu Leu
1 5 10 15
Thr Leu Ile Glu Tyr Cys Thr Trp Leu Ile Ser Cys Pro Ser Leu Lys
20 25 30
Phe Pro Pro Pro Cys Phe Ile Met Pro Thr Phe Trp Ala Gly Tyr Phe
35 40 45
Cys Cys Trp Lys Ser Arg Asn Phe Leu Phe Asn Tyr Asn Tyr Ser Phe
50 55 60
Ile Arg Pro Met Leu Asp Phe Phe Cys Ser Ser Ile Cys Phe Gly Val
65 70 75 80
Leu Arg Ile Tyr Arg Phe Leu Ile Thr Thr Phe Ser Thr Asp Lys Asp
85 90 95
Ser Ser Pro Pro Val Gly Tyr His Asn Thr Pro Thr Phe Gly Ala Glu
100 105 110
Phe His His Leu Ser His Pro His Thr Ser Gln Gly Gln Ser Leu Ile
115 120 125
Val Gln Asn Lys Lys Phe Thr Leu His His Ser Leu Glu Gly Arg Cys
130 135 140
Met Lys Ile Ser Lys Ile Ile Glu Gln Ile Lys Ser Glu Leu Asp Lys
145 150 155 160
Lys Asp Lys Leu Arg Glu Glu Ala Leu Glu Ile Thr Arg Asp Ile Ile
165 170 175
Arg Leu Ser Gly Asp Ala Ile Lys Ala Met His Arg Gly Glu Leu Glu
180 185 190
Leu Ala His Glu Arg Leu Glu Lys Ala Arg Gly Leu Val Lys Glu Leu
195 200 205
Lys Glu Lys Leu Arg Glu His Pro Asp Leu Tyr Tyr Thr Gly Tyr Val
210 215 220
Gln Asn Ala Asn Gln Glu Phe Val Glu Ala Val Leu Met Tyr His Tyr
225 230 235 240
Leu Thr Asp Arg Glu Phe Pro Ser His Val Asp Leu Gly Val Pro Ser
245 250 255
Gln Asp Tyr Ile Leu Gly Val Gly Asp Phe Ile Gly Glu Leu Arg Arg
260 265 270
Tyr Phe Leu Ile Asn Leu Met Lys Gly Asn Leu Asp Glu Ala Glu Ser
275 280 285
Thr Tyr Arg Phe Met Glu Glu Val Tyr Glu Glu Leu Met Thr Leu Glu
290 295 300
Tyr Pro Lys Gly Leu Val Asn Ile Arg Gln Lys Gln Asp Gln Ala Arg
305 310 315 320
Tyr Thr Leu Glu Arg Thr Leu Glu Asp Leu Thr Arg Ala Lys Val Asn
325 330 335
Arg Arg Val Glu Glu Lys Leu Glu Ala Phe Leu Asn Asp Arg Ser
340 345 350
<210> 138
<211> 207
<212> PRT
<213> Pyrococcus furiosus
<400> 138
Met Lys Ile Ser Lys Ile Ile Glu Gln Ile Lys Ser Glu Leu Asp Lys
1 5 10 15
Lys Asp Lys Leu Arg Glu Glu Ala Leu Glu Ile Thr Arg Asp Ile Ile
20 25 30
Arg Leu Ser Gly Asp Ala Ile Lys Ala Met His Arg Gly Glu Leu Glu
35 40 45
Leu Ala His Glu Arg Leu Glu Lys Ala Arg Gly Leu Val Lys Glu Leu
50 55 60
Lys Glu Lys Leu Arg Glu His Pro Asp Leu Tyr Tyr Thr Gly Tyr Val
65 70 75 80
Gln Asn Ala Asn Gln Glu Phe Val Glu Ala Val Leu Met Tyr His Tyr
85 90 95
Leu Thr Asp Arg Glu Phe Pro Ser His Val Asp Leu Gly Val Pro Ser
100 105 110
Gln Asp Tyr Ile Leu Gly Val Gly Asp Phe Ile Gly Glu Leu Arg Arg
115 120 125
Tyr Phe Leu Ile Asn Leu Met Lys Gly Asn Leu Asp Glu Ala Glu Ser
130 135 140
Thr Tyr Arg Phe Met Glu Glu Val Tyr Glu Glu Leu Met Thr Leu Glu
145 150 155 160
Tyr Pro Lys Gly Leu Val Asn Ile Arg Gln Lys Gln Asp Gln Ala Arg
165 170 175
Tyr Thr Leu Glu Arg Thr Leu Glu Asp Leu Thr Arg Ala Lys Val Asn
180 185 190
Arg Arg Val Glu Glu Lys Leu Glu Ala Phe Leu Asn Asp Arg Ser
195 200 205
<210> 139
<211> 204
<212> PRT
<213> Pyrococcus furiosus
<400> 139
Met Lys Gly Leu Ile Ala Phe Asp Leu Glu Gly Thr Leu Thr Asp Met
1 5 10 15
Ile Ser Trp Glu Ile Leu His Glu Lys Phe Gly Thr Cys Asp Lys Ala
20 25 30
Arg Val His Thr Ser Leu Phe Leu Ser Gly Lys Ile Thr Tyr His Glu
35 40 45
Trp Ala Glu Met Asp Val Arg Leu Trp Lys Gly Arg Arg Arg Glu Glu
50 55 60
Val Glu Glu Ala Phe Ser Gln Val Thr Leu Lys Pro Tyr Ala Arg Glu
65 70 75 80
Leu Phe Glu Trp Leu Lys Lys Asn Asn Phe Lys Thr Ala Ile Ile Ser
85 90 95
Gly Gly Leu Met Cys Leu Ala Arg Lys Val Gly Glu Lys Leu Gly Val
100 105 110
Asp Phe Ile Val Ala Asn Glu Leu Lys Phe Asp Ser Gln Gly Arg Ile
115 120 125
Glu Gly Val Ile Val Arg Val Thr Phe Asp Asn Lys Gly Glu Ile Leu
130 135 140
Arg Gln Leu Lys Gln Lys Val Asn Pro Asn Val Thr Ile Ala Val Gly
145 150 155 160
Asp Trp Lys Asn Asp Lys Ser Met Phe Glu Glu Ala Asp Ile Ser Ile
165 170 175
Ser Leu Gly Asp Ile Asp Gly Asp Tyr Lys Ala Arg Asp Leu Arg Asp
180 185 190
Val Leu Glu Ile Leu Lys Ser Ile Val Arg Asn Ile
195 200
<210> 140
<211> 235
<212> PRT
<213> Pyrococcus furiosus
<400> 140
Met Ala Ile Thr Leu Lys Leu Leu Gln Met Cys Ser Ser Leu Met Ile
1 5 10 15
Ile Ala Phe Asp Phe Asp Gly Thr Leu Val Asp Ser Tyr Ser Cys Ile
20 25 30
Glu Glu Ala Phe Tyr Arg Ala Leu Lys Arg Thr Tyr Pro Trp Leu Pro
35 40 45
Gly Lys Arg Tyr Ile Ala Lys Leu Leu Thr Lys Leu Glu Leu Gln Phe
50 55 60
Glu Arg Pro Lys Phe Gly Lys His Gly Arg Lys Ile Lys Pro Pro Met
65 70 75 80
Lys Ile Phe Gln Gly Lys Phe Ala Arg Ala Trp Phe Glu Glu Arg Ala
85 90 95
Lys Leu Thr Lys Pro Leu Asp Gly Ala Arg Glu Val Leu Glu Arg Leu
100 105 110
Lys Glu Asp Gly His Ile Val Ile Ser Phe Ser Ala Glu Asp Phe Ile
115 120 125
Pro Gly Ile Lys Glu Tyr Arg Leu Lys Ile Ser Gly Leu Tyr Asp Leu
130 135 140
Phe Asp Asp Val Ile Ile Phe Gly Arg Asp Ile Thr Ile Cys Glu Ala
145 150 155 160
Phe Ser Ile Val Arg Glu Lys Tyr Gly Tyr Asp Thr Phe Val Trp Val
165 170 175
Asp Asp Lys Pro Trp Arg Phe Ile Gly Arg Gly Asp Glu Asn Thr Glu
180 185 190
Tyr Val Trp Met Tyr Phe Pro Tyr Thr Ala Arg Phe Val Ser Asp Asp
195 200 205
Ile Leu Ala Gln Ile Pro His Leu His Val Ile Tyr Asp Leu Trp Ser
210 215 220
Leu Leu Asp Val Val Lys Asn Leu Lys Ser Arg
225 230 235
<210> 141
<211> 211
<212> PRT
<213> Pyrococcus furiosus
<400> 141
Met Leu Ile Ile Leu Asp Leu Asp Asp Thr Leu Cys Asn Thr Trp Glu
1 5 10 15
Ala Ile Arg Ile Ala Ala Ile Arg Leu Ile Pro Thr Leu Leu Arg Leu
20 25 30
Arg Lys Phe Arg Met Phe Ala Tyr Ile Leu Thr Lys Arg Tyr Arg Glu
35 40 45
Leu Glu Glu Ile Arg Glu Leu His Ile Leu Gly Phe Glu Glu Ile Leu
50 55 60
Asp Arg Ile Ile Asn Lys Ile Tyr Lys Asn Leu Glu Asn Glu Glu Val
65 70 75 80
Gln Glu Ile Ala Asn Leu Phe Asp Arg Thr Phe Phe Ala Asn Leu Arg
85 90 95
Leu Tyr Pro Asp Val Ile Pro Phe Leu Glu Lys Val Arg Glu Met Gly
100 105 110
Ala Lys Ile Val Leu Val Thr Asp Ser Ser Ser Ser Trp Gln Arg Arg
115 120 125
Lys Leu Glu Val Leu Gly Ile Lys Asp Tyr Phe Asp Ala Ile Ile Val
130 135 140
Ser Gly Asp Thr Gly His Ser Lys Phe Glu Pro Tyr Asn Phe His Leu
145 150 155 160
Ala Arg Lys Lys Phe Pro Arg Glu Lys Lys Val Tyr Val Ile Gly Asp
165 170 175
Arg Asp Asp Thr Asp Met Lys Gly Gly Lys Ala Ile His Ala Thr Thr
180 185 190
Ile Leu Val Lys Arg Ala Thr Leu Lys Glu Gly Gly Gln Ser Met Gln
195 200 205
Ile Met Leu
210
<210> 142
<211> 238
<212> PRT
<213> Pyrococcus furiosus
<400> 142
Met Trp Ile Val Phe Asp Val Asp Gly Val Leu Ile Asp Thr Ser Glu
1 5 10 15
Ser Tyr Asp Met Ala Thr Lys Leu Thr Val Glu Tyr Phe Leu Ser His
20 25 30
Leu Gly Ile Lys Lys Glu Val Ser Leu Glu Ile Ile Arg Asp Met Arg
35 40 45
Lys Lys Gly Val Phe Ser Asp Asp Phe Glu Leu Ser Glu Ala Leu Ile
50 55 60
Leu Met Tyr Leu Ser Gly Glu Asp Leu Pro Lys Gly Glu Gly Val Glu
65 70 75 80
Tyr Leu Arg Gln Lys Leu Gly Ile Val Leu Asp Arg Lys Asp Ile Glu
85 90 95
Arg Val Phe Asn Thr Phe Tyr Leu Gly Glu Ile Tyr Pro Asn Ser Ile
100 105 110
Phe Lys Phe Glu Gly Leu Trp Arg Lys Glu Lys Arg Ile Val Asp Ile
115 120 125
Glu Leu Leu Lys Lys Ala Lys Glu Thr Tyr Lys Leu Gly Ile Val Thr
130 135 140
Gly Arg Asp Ser Leu Glu Met Lys Leu Ala Glu Glu Ile Ile Arg Phe
145 150 155 160
Lys Phe Asp Lys Val Val Thr Arg Asp Met Phe Glu Lys Pro Asp Pro
165 170 175
Arg Ala Leu Tyr Glu Val Thr Gln Gly Glu Glu Ala Ile Tyr Ile Gly
180 185 190
Asp Ser Lys Val Asp Glu Glu Leu Val Glu Arg Tyr Arg Arg Thr Phe
195 200 205
Lys Gly Asn Val Thr Tyr Leu Met Val Gly Arg Asp Val Asn Asp Val
210 215 220
Asn Glu Ala Leu Lys Lys Ile Leu Phe Gln Ser Tyr Ile Arg
225 230 235
<210> 143
<211> 219
<212> PRT
<213> Pyrococcus furiosus
<400> 143
Met Val Arg Val Ile Phe Phe Asp Ile Asp Gly Thr Leu Leu Thr Glu
1 5 10 15
Trp Pro Leu Val Lys Leu Met Leu Pro Gln Val Tyr Glu Met Leu Ala
20 25 30
Lys Lys Leu Gly Val Ser Lys Lys Glu Ala Arg Glu Ile Phe Leu Gly
35 40 45
Glu Ile Glu Lys Arg Lys Gly Thr Tyr Glu Trp Tyr Asp Trp Asn Phe
50 55 60
Phe Phe Ser Tyr Phe Asn Leu Pro Leu Arg Tyr Glu Asp Phe Ile Arg
65 70 75 80
Lys Tyr Pro Glu Lys Ile Glu Leu Tyr Pro Gly Val Arg Glu Val Leu
85 90 95
Lys Glu Leu Ser Gly Lys Tyr Lys Leu Gly Ile Ile Thr Ser Gly Pro
100 105 110
His Tyr Gln Leu Leu Lys Leu Lys Val Thr Asp Ile Asp Lys Phe Phe
115 120 125
Asp Val Ile Ile Thr Arg Asp Asp Val Lys Ala Val Lys Pro Ser Pro
130 135 140
Lys Ile Phe Leu Ala Gly Leu Glu Arg Val Arg Ala Lys Pro Thr Glu
145 150 155 160
Ser Leu Met Val Gly Asp Ser Leu Glu Asn Asp Ile Leu Gly Ala Lys
165 170 175
Ala Leu Gly Phe Lys Thr Val Trp Ile Asn Arg Gly Arg Glu Lys Gly
180 185 190
Phe Asn Leu Pro Asp Phe Glu Ile Tyr Glu Met Lys Glu Leu Ile Arg
195 200 205
Val Val Glu Val Ala Glu Asn Glu Lys Asp Ile
210 215
<210> 144
<211> 230
<212> PRT
<213> Pyrococcus furiosus
<400> 144
Met Ser Phe Leu Ala Ile Tyr Lys Thr Asp Val Lys Leu Leu Arg Arg
1 5 10 15
Asp Pro Met Leu Leu Tyr Ser Val Ile Met Val Phe Val Leu Leu Leu
20 25 30
Ile Val Arg Tyr Phe Lys Asp Lys Val Gly Ile Tyr Tyr Tyr Pro Leu
35 40 45
Ala Leu Leu Thr Met Leu Phe Ile Pro Met Ile Phe Gly Met Leu Pro
50 55 60
Gly Phe Val Met Ala Asp Glu Lys Glu Glu Lys Thr Ile Gln Ala Leu
65 70 75 80
Gln Val Ile Pro Ile Ser Ser Glu Ala Phe Leu Ala Tyr Arg Leu Thr
85 90 95
Trp Ala Ser Ile Val Thr Ala Val Leu Gly Phe Ala Ser Pro Tyr Ile
100 105 110
Leu Asp Ile Glu Leu Ser Arg Lys Gly Leu Ala Met Leu Ile Val Leu
115 120 125
Phe Val Leu Glu Val Trp Ile Tyr Gly Leu Leu Ile Thr Val Phe Ser
130 135 140
Glu Ser Arg Met Gln Ala Ile Thr Val Ser Lys Val Leu Gly Trp Phe
145 150 155 160
Leu Met Leu Pro Pro Leu Ile Lys Leu Val Val Leu Trp Arg Asn Leu
165 170 175
Ser Arg Asp Trp Ser Glu Phe Thr Ala Phe Leu Pro Thr Tyr Trp Leu
180 185 190
Tyr Lys Val Phe Glu Gly Ile Leu Gln Asn Asp Tyr Ser Asp Phe Leu
195 200 205
Val Ala Phe Ile Val His Leu Ile Trp Leu Ile Pro Leu Ile Ala Leu
210 215 220
Phe Lys Arg Arg Val Leu
225 230
<210> 145
<211> 240
<212> PRT
<213> Pyrococcus furiosus
<400> 145
Met Arg Arg Ile Lys Val Ile Phe Phe Asp Leu Asp Asp Thr Leu Val
1 5 10 15
Asp Thr Ser Lys Leu Ala Glu Val Ala Arg Lys Asn Ala Ile Glu Asn
20 25 30
Met Ile Arg His Gly Met Pro Val Asp Phe Asp Thr Ala Tyr Asn Glu
35 40 45
Leu Leu Glu Leu Ile Lys Glu Tyr Gly Ser Asn Phe Pro Tyr His Phe
50 55 60
Asp Tyr Leu Leu Arg Arg Leu Asp Leu Glu Tyr Asn Pro Lys Trp Val
65 70 75 80
Ala Ala Gly Val Ile Ala Tyr His Asn Thr Lys Phe Thr Tyr Leu Arg
85 90 95
Glu Val Pro Gly Ala Arg Lys Thr Leu Leu Arg Leu Lys Lys Glu Gly
100 105 110
Tyr Met Thr Gly Ile Ile Thr Asp Gly Asn Pro Ile Lys Gln Trp Glu
115 120 125
Lys Ile Leu Arg Leu Glu Leu Asp Asp Phe Phe Glu His Val Met Ile
130 135 140
Ser Asp Phe Glu Gly Val Lys Lys Pro His Pro Lys Ile Phe Lys Lys
145 150 155 160
Ala Leu Lys Ala Phe Asn Val Lys Pro Glu Glu Ala Ile Met Val Gly
165 170 175
Asp Arg Leu Tyr Ser Asp Ile Tyr Gly Ala Lys Asn Val Gly Met Lys
180 185 190
Thr Val Trp Phe Lys Tyr Gly Lys Tyr Ala Glu Leu Asp Leu Glu Tyr
195 200 205
Lys Glu Tyr Ala Asp Tyr Val Ile Thr Glu Leu Pro Gln Leu Leu Glu
210 215 220
Val Leu Glu Arg Glu Asn Gly Ser Asp Lys Glu Val His Ser Ser Gly
225 230 235 240
<210> 146
<211> 218
<212> PRT
<213> Pyrococcus furiosus
<400> 146
Met Ile Lys Gly Val Ile Phe Asp Val Asp Glu Thr Leu Val Tyr Tyr
1 5 10 15
Glu Gly Tyr Ser Leu Lys Glu Trp Tyr Glu Lys Ile Ala Lys Pro Thr
20 25 30
Met Lys Lys Leu Gly Ile Leu Ile Asp Trp Glu Thr Phe Arg Lys Met
35 40 45
Ala Lys Gly Glu Leu Pro Arg Ser Tyr Val Glu Glu Phe Gly Ile Asp
50 55 60
His Val Thr Phe Trp Lys Ala Leu Asp Lys Ala Asn Arg Arg Tyr Arg
65 70 75 80
Glu Glu Leu Leu Asn Glu Gly Lys Ile Arg Thr Tyr Asp Asp Val Glu
85 90 95
Ala Ile Lys Glu Leu Lys Lys Leu Gly Leu Lys Leu Ala Ala Val Ser
100 105 110
Asn Ala Ser Gln Asp Asn Thr Glu Leu Val Leu Lys Ala Phe Asn Leu
115 120 125
Leu Asp Tyr Phe Asp Val Val Tyr Gly Lys Asp Tyr Thr Tyr Leu Asp
130 135 140
Gly Val Lys Pro Asn Pro Tyr Leu Ile Asn Lys Ala Leu Asn Thr Leu
145 150 155 160
Gly Leu Arg Pro Asp Glu Ala Ile Leu Val Gly Asp Ser Asp Leu Asp
165 170 175
Ile Ile Ala Gly Lys Arg Ala Gly Val Thr Val Val Gln Ile Val Arg
180 185 190
Glu Lys Lys Tyr Glu Gly Ala Asp Tyr Tyr Ile Gln Asn Leu Trp Glu
195 200 205
Leu Val Asp Leu Ile Lys Arg Ser Arg Cys
210 215
<210> 147
<211> 234
<212> PRT
<213> Pyrococcus furiosus
<400> 147
Met Ile Lys Ala Val Phe Phe Asp Phe Ile Gly Thr Leu Leu Ser Gln
1 5 10 15
Glu Gly Glu Tyr Glu Thr His Leu Lys Ile Met Glu Glu Val Leu Gly
20 25 30
Glu Asn Lys Lys Ile Ser Pro Glu Glu Leu Leu Arg Glu Tyr Asp Ala
35 40 45
Leu Thr Arg Glu Ala Phe Ser Gln Tyr Ala Gly Lys Pro Phe Lys Pro
50 55 60
Ile Arg Ile Ile Glu Glu Glu Ile Met Lys Lys Leu Ala Glu Lys Tyr
65 70 75 80
Gly Phe Asp Tyr Pro Glu Asn Phe Trp Glu Ile His Leu Lys Ala His
85 90 95
Gln Arg Tyr Gly Lys Leu Tyr Pro Glu Val Val Glu Val Leu Asn Glu
100 105 110
Leu Lys Lys Arg Glu Tyr His Val Gly Leu Ile Thr Asp Ser Asp Thr
115 120 125
Ala Tyr Leu Arg Ala His Leu Glu Ala Leu Gly Ile Ala Glu Leu Phe
130 135 140
Asp Ser Ile Thr Thr Ser Glu Glu Ala Gly Phe Phe Lys Pro His Pro
145 150 155 160
Arg Ile Phe Glu Val Ala Leu Lys Lys Ala Gly Val Lys Gly Ser Glu
165 170 175
Ala Val Tyr Val Gly Asp Asn Pro Ile Lys Asp Cys Gly Gly Ala Arg
180 185 190
Gln Leu Asp Met Leu Ser Ile Leu Val Asp Arg Lys Gly Glu Lys Lys
195 200 205
Glu Leu Trp Lys Glu Cys Glu Phe Val Ile Ser Asp Leu Arg Glu Val
210 215 220
Ile Gln Ile Val Glu Glu Leu Asn Gly Gln
225 230
<210> 148
<211> 254
<212> PRT
<213> Pyrococcus furiosus
<400> 148
Met Lys Leu Lys Phe Trp Arg Glu Val Ala Ile Asp Ile Ile Ser Asp
1 5 10 15
Phe Glu Thr Thr Ile Met Pro Phe Phe Gly Asn Pro Asp Gly Gly Lys
20 25 30
Leu Val Lys Ile Ser Pro Ser Gly Asp Glu Thr Lys Leu Val Asp Lys
35 40 45
Leu Ala Glu Asp Leu Ile Leu Ser Arg Ile Thr Glu Leu Gly Val Asn
50 55 60
Val Val Ser Glu Glu Val Gly Val Ile Asp Asn Glu Ser Glu Tyr Thr
65 70 75 80
Val Ile Val Asp Pro Leu Asp Gly Ser Tyr Asn Phe Ile Ala Gly Ile
85 90 95
Pro Phe Phe Ala Leu Ser Leu Ala Val Phe Lys Lys Asp Lys Pro Ile
100 105 110
Tyr Ala Ile Ile Tyr Glu Pro Met Thr Glu Arg Phe Phe Glu Gly Ile
115 120 125
Pro Gly Glu Gly Ala Phe Leu Asn Gly Lys Arg Ile Lys Val Arg Lys
130 135 140
Thr Pro Asp Glu Lys Pro Ser Ile Ser Phe Tyr Ser Arg Gly Lys Gly
145 150 155 160
His Glu Ile Val Lys His Val Lys Arg Thr Arg Thr Leu Gly Ala Ile
165 170 175
Ala Leu Glu Leu Ala Tyr Leu Ala Met Gly Ala Leu Asp Gly Val Val
180 185 190
Asp Val Arg Lys Tyr Val Arg Pro Thr Asp Ile Ala Ala Gly Thr Ile
195 200 205
Ile Ala Lys Glu Ala Gly Ala Leu Ile Lys Asp Ser Ala Gly Lys Asp
210 215 220
Ile Asp Ile Ser Phe Asn Ala Thr Asp Arg Leu Asp Val Ile Ala Val
225 230 235 240
Asn Ser Glu Glu Leu Leu Lys Thr Ile Leu Ser Leu Leu Glu
245 250
<210> 149
<211> 264
<212> PRT
<213> Pyrodictium occultum
<400> 149
Met Arg Gly Arg Gly Arg Gly Arg Leu Leu Gly Ala Val Leu Ala Gly
1 5 10 15
Val Ala Ser Ala Gly Glu Leu Ala Arg Leu Leu Pro Gly Arg Val Cys
20 25 30
Gly Val Ala Val Asp Ile Asp Gly Thr Ile Thr Glu Arg Arg Val Ser
35 40 45
Gly Asp Phe Arg Leu Ser Leu Glu Ala Val Glu Ala Leu Arg Arg Leu
50 55 60
Glu Asp Ala Gly Val Arg Val Ile Leu Val Thr Gly Asn Ser Val Met
65 70 75 80
Val Ala Ala Gly Val Ala Arg Tyr Ile Gly Val Arg Gly Pro His Val
85 90 95
Ala Glu Asn Gly Cys Leu Val Tyr Gln Arg Gly Ser Val Val His Ala
100 105 110
Cys Arg Gly Thr Ala Arg Ala Ala Ala Glu Ala Leu Glu Glu Glu Leu
115 120 125
Gly Gly Leu Leu Glu Pro Ser Trp Gln Asn Arg Cys Arg Ile His Asp
130 135 140
Tyr Ala Phe Leu Val Arg Arg Val Gly Pro Glu Glu Ala Trp Arg Met
145 150 155 160
Ala Glu Arg Val Leu Arg Glu Arg Gly Leu Arg Ala Lys Leu Ser His
165 170 175
Ser Gly Tyr Ala Leu His Val Arg Pro Leu Glu Ala Ser Lys Gly Leu
180 185 190
Gly Leu Arg Val Ala Met Arg Met Ala Gly Leu Glu Pro Gly Cys Val
195 200 205
Val Ala Val Gly Asp Ser Ala Met Asp Leu Glu Met Arg Asp Ala Gly
210 215 220
Val Thr Leu Ala Ala Val Gly Asn Ala Asp Pro Arg Leu Arg Glu Gly
225 230 235 240
Ala Asp Leu Ile Leu Pro Gly Glu Ser Gly Gly Gly Val Ala Leu Leu
245 250 255
Ala Arg Ala Ile Leu Glu Ser Leu
260
<210> 150
<211> 235
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 150
Met His Leu Leu Leu Phe Asp Ile Asp Gly Thr Leu Ile Arg Thr Arg
1 5 10 15
Gly Phe Gly Arg Gln Thr Met Glu Ala Ala Leu Ser Glu Trp Leu Gly
20 25 30
Arg Pro Val Thr Thr Glu Gly Val Asp Phe Ala Gly Arg Thr Asp Pro
35 40 45
Ala Ile Leu Leu Asp Ile Leu Lys Ala Ser Gly Leu Pro Glu His Thr
50 55 60
Ala Arg His Leu Leu Pro Glu Ala Leu Glu Val Tyr Ser Arg Ala Met
65 70 75 80
Ile Arg Arg Leu Arg Pro Glu His Leu Glu Val Leu Pro Gly Val Val
85 90 95
Met Leu Leu Glu Glu Leu Ser Glu Trp Pro Asp Val Tyr Leu Gly Leu
100 105 110
Val Thr Gly Asn Leu Arg Pro Val Ala Phe His Lys Leu Ala Met Ala
115 120 125
Gly Leu Ala Gly Tyr Phe Gly Glu Gly Ala Phe Gly Cys Asp His Ala
130 135 140
Asn Arg Asn Glu Leu Pro Pro Leu Ala Ile Glu Arg Ile Arg Glu Ala
145 150 155 160
Thr Gly Tyr Pro Phe Thr Gly Ala Asp Ala Val Ile Ile Gly Asp Thr
165 170 175
Pro His Asp Val Ala Cys Ala Arg His Ala Gly Ala Ser Val Ala Val
180 185 190
Val Cys Thr Gly Gly Tyr Ser Arg Asp Ala Leu Glu Ala Cys Arg Pro
195 200 205
Asp Leu Leu Leu Glu Asp Leu Ser Asp Pro Glu Pro Leu Phe Lys Leu
210 215 220
Leu Thr Gln Gln Ala Leu Ser Arg Lys Ala Ser
225 230 235
<210> 151
<211> 279
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 151
Met Phe Asp Leu Arg Arg Ala Asp Leu His Leu His Thr Ser Arg Ser
1 5 10 15
Asp Gly Arg Leu Ser Pro Ala Glu Leu Val Arg Arg Ala Arg Glu Ala
20 25 30
Gly Leu Tyr Cys Val Ala Ile Thr Asp His Asp Thr Ile Asp Gly Leu
35 40 45
Glu Glu Ala Arg Gln Ala Ala Ala Arg Trp Ala Met Val Val Ile Pro
50 55 60
Gly Val Glu Leu Ser Val Gln Val Glu Glu Glu Glu Val His Leu Leu
65 70 75 80
Gly Tyr Phe Phe Asp Pro Asp His Pro Ala Leu Arg Glu Ala Leu Thr
85 90 95
Ala Tyr Arg Lys Ala Arg Glu Glu Arg Leu Ala Ala Met Leu Ala Arg
100 105 110
Leu Gln Glu Val Gly Val Arg Leu Ser Glu Glu Gln Val Gln Thr Ala
115 120 125
Val Gly His Gly Val Pro Gly Arg Pro His Val Ala Arg Ala Leu Val
130 135 140
Ala Ala Gly Tyr Ala Glu Ser Tyr Arg Glu Ala Phe Gln Arg Tyr Leu
145 150 155 160
Leu Pro Gly Gly Pro Gly Tyr Val Pro Lys Pro Ala Trp Thr Ala Glu
165 170 175
Glu Ala Val Ala Val Leu His Glu Ala Gly Gly Ile Ala Val Leu Ala
180 185 190
His Pro Gly Glu His Leu Arg Asp Arg Val Phe Arg Ala Leu Leu Gln
195 200 205
Ala Gly Ile Asp Gly Ile Glu Val Ile His Pro Ala His Ser Tyr Tyr
210 215 220
Leu Val Gln His Tyr Arg Gln Val Ala Arg Asp Phe Gly Leu Leu Glu
225 230 235 240
Thr Gly Gly Ser Asp Tyr His Gly His Arg Pro Glu Asp Asp Ala Leu
245 250 255
Leu Gly Ala Cys Thr Ile Pro Tyr Pro Arg Val Glu Arg Leu Arg Ala
260 265 270
Thr Leu Gln Ala Thr Arg Ala
275
<210> 152
<211> 846
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 152
Met Ser Ser Arg Arg Phe Ala Thr Ser Asp Leu Val Leu Ala Leu Ala
1 5 10 15
Gly Val Ile Gly Leu Val Leu Gly Leu Trp Leu Leu Pro Arg Gln His
20 25 30
Pro Asp Ala Ala Leu Gln His Met Leu Ser Ala Glu Ala Ala Arg His
35 40 45
Arg Ala Ala Ala Phe Leu Ala Gln Arg Gly Tyr Arg Val Asp Thr Thr
50 55 60
His Ala Leu Val Val Leu Arg Arg Ala Pro Glu Leu Leu Arg Arg Trp
65 70 75 80
Gln Thr Arg Trp Gly Arg Pro Glu Leu Val Arg Arg Leu Glu Asp Leu
85 90 95
Pro Trp Leu Pro Val Tyr Arg Trp Val Val Tyr Arg Ser Ser Asp Glu
100 105 110
Met Asn Gly Gln Arg Trp Gln Val Val Leu Ala Gly Asp Gly Thr Ile
115 120 125
Trp Gly Phe Arg Gly Pro Glu Thr Pro Gly Glu Asp Pro Ala Ala Arg
130 135 140
Ala Ala Val Gly Ile Ala Ala Glu Ala Phe Asp Pro Leu Ala Ser Trp
145 150 155 160
Gly Lys Leu Ser Gly Ala Pro Asp Ser Ser Glu Thr Pro Glu Arg Pro
165 170 175
Gly Pro Ala Ala Ala Val Ala Leu Ala Arg Tyr His Leu Gln Arg Thr
180 185 190
Ile Gly Ser Val Leu Pro Leu Arg Pro Asp Ser Val Gly Leu Leu Ala
195 200 205
Thr Ser Ser Pro Gln Gln Ala Val Val Arg Phe Arg Gly Leu Ser Pro
210 215 220
Thr Gly Asp Ser Val Thr Val Gln Val Val Val Thr Ala Ser Gly Gln
225 230 235 240
Leu Arg Ala Leu Glu Ala Thr Trp Gly Thr Leu His Leu Pro Glu Pro
245 250 255
Ser Pro Leu Glu Glu Gly Pro Pro Arg Arg Gly Glu Arg Gly Phe Thr
260 265 270
Val His Glu Val Gln Asp Leu Leu Ser Val Leu Leu Phe Ala Gly Leu
275 280 285
Gly Ile Trp Leu Leu Thr Val Phe Leu Arg Arg Leu His Arg Arg Leu
290 295 300
Leu Asp Thr Gln Gly Pro Leu Arg Asp Ala Val Leu Gly Gly Leu Ala
305 310 315 320
Phe Thr Val Ala Thr Leu Gly Gly Ala Leu Pro Gly Ile Leu Gln Val
325 330 335
Pro Asp Leu Trp Leu Arg Leu Leu Ile Leu Leu Met Ser Thr Leu Val
340 345 350
Val Gly Val Phe Gly Ala Ala Ser Val Phe Leu Leu Ala Gly Thr Ser
355 360 365
Asp Ala Leu Ala Arg Asp Arg Trp Pro Glu Lys Leu Ala Val Leu Thr
370 375 380
Leu Leu Arg Asn Gly Gln Val Arg Asn Val Val Val Gly Ala Ser Leu
385 390 395 400
Leu Arg Gly Leu Ala Leu Gly Gly Leu Leu Leu Gly Met Thr Val Gly
405 410 415
Leu Leu Gly Leu Trp Pro Arg Ala Ala Leu Arg Leu Asp Ala Gly Ala
420 425 430
Trp Thr Val Pro Trp Pro Gly Met Gln Ala Ala Val Trp Leu Gly Phe
435 440 445
Ala Val Trp Val Gly Met Leu Leu Val Tyr Leu Leu Leu Ala Val Met
450 455 460
Ala Arg Leu Pro Trp Arg Asp Gly Gly Arg Val Val Gly Thr Leu Thr
465 470 475 480
Leu Leu Leu Leu Leu Ala Gly Val Ser Ala Ile Asp Leu Glu Gln Val
485 490 495
Gly Phe Glu Leu Leu Val His Ala Leu Trp Gly Val Val Leu Ala Trp
500 505 510
Ala Cys Trp Arg Tyr Glu Pro Ala Cys Ser Gly Ile Gly Ala Met Thr
515 520 525
Ala Trp Ala Leu Trp Arg Ser Ala Ser Gly Trp Met Ala Pro Asp Gly
530 535 540
Pro Phe Gly Pro Asp Gly Trp Ile Val Leu Ala Met Val Gly Thr Gly
545 550 555 560
Leu Val Ile Gly Phe Val Gly Ile Arg Ser Arg Arg Ser Ala Ala Glu
565 570 575
Leu Pro Arg Tyr Val Pro Ala Tyr Leu Gln Glu Leu Ala Arg Gln Glu
580 585 590
Arg Leu Glu Arg Glu Leu Glu Ile Ala Arg Gln Ala Gln Ala Ser Leu
595 600 605
Leu Pro Arg Thr Leu Pro Glu Val Pro Gly Ala Ala Met Ala Ala Leu
610 615 620
Cys Arg Pro Ala Tyr Glu Val Gly Gly Asp Tyr Tyr Asp Val Phe Ala
625 630 635 640
Leu Pro Asp Gly Arg Leu Ala Val Val Val Gly Asp Val Ser Gly Lys
645 650 655
Gly Ile Gln Ala Ala Phe Phe Met Thr Leu Ile Lys Gly His Val Arg
660 665 670
Ala Leu Ser Leu Ser Thr Arg Asp Pro Ala Asp Val Leu Arg His Leu
675 680 685
Asn Arg Leu Phe Arg Glu Gln Ala Pro Arg Gly Leu Phe Val Thr Met
690 695 700
Ile Tyr Gly Val Leu Asp Pro Thr Thr Arg Thr Phe Thr Leu Ala Arg
705 710 715 720
Ala Gly His Pro Pro Val Leu His Tyr Arg Ala Cys Thr Gln Gln Val
725 730 735
Gln Cys Leu Arg Pro Ser Gly Met Gly Ile Gly Leu Ala Asp Ala Glu
740 745 750
Leu Phe Asp Lys Ala Leu Glu Asn Cys Val Leu Arg Leu Glu Ala Gly
755 760 765
Asp Arg Val Leu Leu Tyr Thr Asp Gly Ile Thr Glu Met Ala Gly Pro
770 775 780
Arg Gly Glu Arg Trp Gly Leu Glu Arg Leu Gln Gln Trp Leu Arg Glu
785 790 795 800
Ser Ser Arg Arg Gly Tyr Ala Pro Glu Gln Ala Leu Gly Ala Leu Glu
805 810 815
Glu Gln Leu Arg Ala Phe Ala Gly Thr Thr Glu Leu Ala Asp Asp Leu
820 825 830
Thr Ala Ile Leu Leu Glu Ala Lys Gln Asp Gly Tyr Ala Asn
835 840 845
<210> 153
<211> 534
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 153
Met Pro Ala Val Val Ala Glu Leu Glu Gln Ala Thr Arg Ser Pro Val
1 5 10 15
Arg Met Cys Val Ile Asp Leu Gly Thr Asn Ser Phe His Ala Val Ile
20 25 30
Val Asp Ala Leu Pro Gly Thr Phe Arg Val Val Asp Arg Phe Lys Glu
35 40 45
Met Val Arg Leu Gly Glu Glu Gly Leu Gln Gly Gln Arg Leu Ser Glu
50 55 60
Ala Ala Met Gln Arg Ala Ile Arg Ala Leu Arg Arg Ile Arg Gln Leu
65 70 75 80
Ala Glu Gly Trp Gly Ala Thr Glu Phe Leu Ala Cys Ala Thr Ser Ala
85 90 95
Ile Arg Glu Ala Glu Asn Gly Gly Glu Leu Leu Gln Arg Ile Arg Ser
100 105 110
Glu Val Gly Leu His Val Arg Val Ile Asp Gly Leu Gln Glu Ala Arg
115 120 125
Leu Ile Tyr Lys Gly Val Arg Arg Ala Val Pro Met Pro Glu Pro Val
130 135 140
Leu Ile Met Asp Val Gly Gly Gly Ser Val Glu Phe Ile Val Gly Thr
145 150 155 160
Ser Gln Gln Pro Leu His Leu Phe Ser Leu Lys Leu Gly Ala Ala Arg
165 170 175
Met Thr Arg Arg Phe Val Arg His Asp Pro Ala Thr Arg Glu Glu Leu
180 185 190
Lys Ala Leu Arg Ala Phe Tyr Arg Glu Gln Leu His Pro Val Phe Glu
195 200 205
Ala Ala Arg Ala His Gly Val Arg Glu Val Val Gly Ser Ser Gly Ala
210 215 220
Met Glu Asn Leu Ala Ser Val Cys Ala Arg Met Arg Gly Glu Val Ala
225 230 235 240
Arg Ser Ile Tyr Glu Gln Pro Phe Pro Ala Glu Asp Phe Arg Arg Val
245 250 255
Ala Arg Arg Ile Met Arg Leu Ser Arg Glu Gln Arg Arg Arg Leu Arg
260 265 270
Gly Ile Asp Pro Lys Arg Val Asp Gln Ile Val Ala Ala Ala Ala Leu
275 280 285
Ile Asp Val Val Leu Lys Asp Leu Ala Ile Glu Arg Val Arg Ile Ser
290 295 300
Pro His Ala Leu Arg Glu Gly Leu Val Val Asp Phe Val Glu Arg Asn
305 310 315 320
Ala Pro Leu Leu Ala Arg Leu Thr Ala Phe Ala Asp Val Arg Arg Arg
325 330 335
Ser Ile Tyr Glu Met Gly Trp Arg Phe Asp Trp Glu Tyr Arg His Ala
340 345 350
Gln Gln Val Ala Ala Leu Ala Leu Gln Leu Phe Asp Ala Thr Gln Ser
355 360 365
Leu His Gly Leu Gly His Val Asp Arg Glu Leu Leu Glu Tyr Ala Ala
370 375 380
Leu Leu His Asp Ile Gly Tyr His Ile Ser His Arg Ser His His Lys
385 390 395 400
His Gly Leu Tyr Leu Ile Lys His Ala Asp Leu Arg Gly Phe Thr Ser
405 410 415
Glu Glu Ile Ala Val Leu Ala Asn Val Val Arg Tyr His Arg Gly Ser
420 425 430
Leu Pro Lys Pro Thr His Ala Asp Tyr Met Ala Leu Ser Glu Glu Asn
435 440 445
Arg Val Arg Val Cys Lys Leu Ala Ala Leu Leu Arg Leu Ala Glu Gly
450 455 460
Leu Asp Arg Ser His Asn Gln Asn Val Arg Thr Leu His Val Arg Leu
465 470 475 480
Glu Ser Asp Arg Leu Val Leu His Leu Glu Thr Arg Gly Asp Pro Glu
485 490 495
Leu Glu Val Trp Gly Val Arg Arg Ser Ala Glu Leu Phe Glu Gln Thr
500 505 510
Phe Gly Arg Thr Val Glu Val Ser Ala Thr Ala Glu Pro Pro Leu Leu
515 520 525
Glu Glu Thr Ala Gly Ala
530
<210> 154
<211> 336
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 154
Met Thr Thr Asn His Ile Gln Glu Leu Leu Ser Lys Leu Asn Glu Leu
1 5 10 15
Arg Ala Val Phe Ile Leu Gly Gln Arg Ala Ile Pro Phe Ile Glu Glu
20 25 30
Val Val Tyr Phe Leu Arg Glu Ile Ser Pro Leu Leu Ser Glu Val Asn
35 40 45
Asp Ser Leu Ala Glu Ser Thr Arg Lys Met Pro Arg Ala Ser Ser Gln
50 55 60
Leu Lys Ser Val Thr Gln Ala Thr Glu Met Ala Thr Thr Glu Ile Leu
65 70 75 80
Asp Leu Ile Asp Val Val Leu Asn Asp Leu Asp Thr Phe Arg Lys Ser
85 90 95
Trp Ala Ala Ala Pro Glu Ala Leu Gln Thr Leu Gln Ser Gln Glu Glu
100 105 110
Gln Leu Trp Gln Pro Leu Pro Glu Ser Trp Asn Glu Arg Leu Gln Thr
115 120 125
Leu Leu Glu Glu Arg Arg Gln His Tyr Ala Thr Leu Gln Ala Met Leu
130 135 140
Glu Ser Gln Gln Gln Leu Val Asp Ser Leu Arg Asp Arg Met Asn Arg
145 150 155 160
Ile Met Met Ala Leu Gln Val Gln Asp Ile Thr Ala Gln Gln Leu Ala
165 170 175
Ala Val Asn His Leu Ile Glu Ser Val Arg Tyr Arg Met Ala Gln Leu
180 185 190
Ile Arg Arg Leu Gly Ser Glu Val Leu Asp Asp Leu Glu Leu Pro Arg
195 200 205
Gln Leu Phe Ala Glu Gly Thr Phe Asp Pro His Ala Arg Tyr Asp Arg
210 215 220
Asp Gly Arg Arg Gln Gln Gln Val Asp Ala Leu Val Asp Ala Leu Gln
225 230 235 240
His Asn Ala Ser Leu Pro Glu Asp Gly Glu Ser Thr Gly Pro Ala Ser
245 250 255
Gln Asp Glu Ile Asp Ala Leu Phe Ser Gly Asn Ser Thr Pro Ala Ser
260 265 270
Gln Asp Glu Ile Asp Ser Leu Phe Ser Ser Gly Thr Pro Thr Ser Gln
275 280 285
Asp Glu Ile Asp Ala Leu Phe Gly Gly Gly Ser Thr Pro Ala Ser Gln
290 295 300
Asp Glu Ile Asp Ala Leu Phe Gly Gly Gly Ser Thr Pro Ala Ser Gln
305 310 315 320
Asp Glu Ile Asp Ala Leu Phe Asn Pro Lys Arg Asn Asp Ser Asn Ser
325 330 335
<210> 155
<211> 231
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 155
Met Ser Leu Leu Phe Thr Glu Glu Glu Leu Thr Arg Val Arg Glu Ala
1 5 10 15
Val Ala Ala Ala Glu Gln Arg Thr Ala Gly Glu Ile Val Pro Val Ile
20 25 30
Val Pro Arg Ser Gly Ala Tyr Pro Glu Ala Val Trp Lys Gly Ala Val
35 40 45
Leu Leu Met Leu Pro Val Leu Ala Val Ala Leu Leu Phe Asp Tyr Ile
50 55 60
Tyr Gln Gly Trp Gly Leu Thr Leu Leu His Thr Gly Trp Gly Val Ala
65 70 75 80
Leu Leu Thr Ala Leu Ala Gly Leu Val Gly Gly Leu Leu Gly Ala Tyr
85 90 95
Val Ala Pro Val Gln Arg Trp Leu Val Gly Glu Ala Arg Met Ala Glu
100 105 110
Gln Val His Leu Arg Ala Leu Gln Ala Phe Leu Glu Glu Glu Val Phe
115 120 125
Asn Thr Arg Asp Arg Thr Gly Ile Leu Ile Phe Val Ser Leu Phe Glu
130 135 140
His Trp Val Glu Val Ile Gly Asp Ala Gly Ile Asn Gln Arg Val Gly
145 150 155 160
Pro Glu Ala Trp Ala Glu Val Val Asp Arg Ile Arg Lys Gly Ile Arg
165 170 175
Glu Gly Arg Pro Val Asp Gly Leu Val Ala Ala Ile Glu Gln Cys Gly
180 185 190
Gln Leu Leu Ala Thr His Gly Val Ala Leu Arg Pro Asp Asp Thr Asn
195 200 205
Glu Leu Ala Asp Thr Leu Arg Val Arg Gly Ala Pro Ser Lys Lys Arg
210 215 220
Arg Gly Gly Arg Arg Lys Arg
225 230
<210> 156
<211> 221
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 156
Met Pro Ala Thr Thr Leu Tyr Phe Val Arg His Gly Glu Thr Asp Tyr
1 5 10 15
Asn Arg Asn Gly Ile Val Gln Gly Arg Gly Val Asp Ala Pro Leu Asn
20 25 30
Glu Arg Gly Arg Arg Gln Ala Glu Ala Leu Ala Arg Arg Phe Ala Ala
35 40 45
Val Pro Leu Asp Ala Ile Tyr Ala Ser Pro Leu Arg Arg Ala Leu Glu
50 55 60
Thr Ala Glu Ala Val Arg Arg Tyr His Pro Glu Val Pro Phe Tyr Gln
65 70 75 80
Leu Ala Asp Leu Glu Glu Met Asp Trp Gly Asp Leu Glu Gly Lys Pro
85 90 95
Tyr Ala Pro Pro Tyr Asp Ala Lys Ile Arg Ala Ile Tyr Glu Arg Trp
100 105 110
Arg Ala Gly Asp Tyr Asp Tyr Pro Val Pro Gly Gly Glu Ser Ile Leu
115 120 125
Asp Val Gln Arg Arg Ala Leu Arg Ala Leu Glu Thr Ile Leu Ser Arg
130 135 140
His Glu Gly Glu Thr Val Leu Ile Val Ala His Gly Arg Phe Leu Arg
145 150 155 160
Ile Leu Leu Ala Ser Val Leu Ser Glu Tyr Gly Leu Ala Arg Met Glu
165 170 175
Ala Leu Pro His Thr Asn Thr Ala Val Asn His Leu Val Tyr Glu Asn
180 185 190
Gly Arg Phe Arg Ala Leu Arg Leu Asn Cys Thr Ala His Leu Glu Glu
195 200 205
Ala Ala Val Asp Asp Gly Leu Pro Thr Glu Val Ala Ala
210 215 220
<210> 157
<211> 162
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 157
Met Arg Leu Cys Leu Leu Arg His Ala Glu Ala Tyr Pro Ala Ala Pro
1 5 10 15
Gly Arg Pro Asp Ala Glu Arg Ser Leu Thr Glu Val Gly Gln Gln Val
20 25 30
Ala Arg Gln Met Gly Glu Ala Leu Arg Arg Leu Arg Leu Ala Pro Gly
35 40 45
Ala Val Tyr Thr Ser Pro Tyr Arg Arg Ala Val Gln Thr Ala Gln Ala
50 55 60
Val Ala Glu Ala Leu Gly Val Pro Val Val Glu Asp Arg Leu Leu Ala
65 70 75 80
Pro Gly Cys Gly Pro Ala Glu Leu Glu Thr Leu Ile Gln Ala Tyr Ala
85 90 95
Pro Gly Glu Thr Val Leu Val Val Gly His Gln Pro Asp Phe Gly Glu
100 105 110
Leu Val Arg Trp Leu Thr Gly Ala Thr Ile Arg Leu Pro Ala Gly Gly
115 120 125
Leu Ala Val Val Glu Thr Pro Ala Leu Arg Glu Arg Ala Gly Thr Leu
130 135 140
His Gly Leu Tyr Asp Pro Ala Trp Leu Ala Ala Val Met Thr Gly Arg
145 150 155 160
Thr Gly
<210> 158
<211> 249
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 158
Met Asn Val Glu Val Phe Leu Thr Gly Ser His Val Thr Glu Glu Asp
1 5 10 15
Val Glu Gly Arg Thr Val Val Val Ile Asp Val Leu Arg Ala Ser Ser
20 25 30
Thr Ile Ile Thr Ala Leu Ala Asn Gly Ala Arg Ala Val Ile Pro Val
35 40 45
Ala Asp Met Asp Gln Ala Gly Lys Ile Ala Met Asn Leu Asp Pro Ser
50 55 60
Thr Tyr Leu Leu Gly Gly Glu Arg Asp Gly Asp Arg Ile Asp Gly Tyr
65 70 75 80
His Leu Gly Asn Ser Pro Leu Glu Tyr Thr Pro Asp Val Val Glu Gly
85 90 95
Lys Thr Ile Ile Leu Asn Thr Thr Asn Gly Thr Arg Thr Ile Trp Asn
100 105 110
Ala Arg Asn Ala Glu His Leu Ile Val Gly Gly Phe Leu Asn Ala Asn
115 120 125
Arg Val Val Gln Phe Val Arg Glu Ala Gly Leu Asp Val Thr Ile Ile
130 135 140
Cys Ala Gly Arg Asn Asn Arg Val Ala Leu Asp Asp Ala Leu Cys Ala
145 150 155 160
Gly Leu Leu Leu His Arg Leu Trp Glu Gly Arg Glu Pro Glu Tyr Val
165 170 175
Ser Asp Ala Ala His Ile Ala Leu Thr Gln Tyr Leu His Asp Arg Asp
180 185 190
Arg Leu Ala Asp Ala Leu Arg His Ser Asn His Ala Arg Trp Leu Ile
195 200 205
Glu Lys Gly Tyr Gly Ala Asp Val Glu Tyr Cys Leu Gln Leu Asp Ala
210 215 220
Leu Pro Val Leu Pro Tyr Tyr Arg Glu Asn Arg Leu Val Leu Tyr Arg
225 230 235 240
Glu Arg Gln Pro Ser Glu Ser Val Gly
245
<210> 159
<211> 345
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 159
Met Glu Thr Leu His Thr Arg Gly Arg Lys Val Val Glu Glu Phe Ile
1 5 10 15
Thr Leu Glu Gln Phe Ile Ile Asp Gln Gln Glu Arg Phe Pro His Ser
20 25 30
Thr Gly Ala Phe Ser Arg Leu Leu Arg Asp Ile Ser Val Ala Ala Lys
35 40 45
Ile Val Asn Arg Asp Ile Arg Arg Ala Gly Leu Val Asp Ile Phe Gly
50 55 60
Thr Thr Gly Lys Val Asn Ile His Gly Glu Val Gln Gln Lys Leu Asp
65 70 75 80
Ala Leu Ala His Glu Glu Phe Val Arg Ala Leu Arg Arg Gly Gly Glu
85 90 95
Cys Cys Leu Ile Gly Ser Glu Glu His Ala Glu Ala Ile Pro Leu Ser
100 105 110
Ala Asn Gly Glu Gly Asp Gly Arg Tyr Ile Val Leu Leu Asp Pro Leu
115 120 125
Asp Gly Ser Ser Asn Val Asp Val Asn Val Ser Val Gly Thr Ile Phe
130 135 140
Ser Ile Tyr Arg Leu Pro Asp Glu Tyr Glu Thr Pro Thr Leu Glu Ala
145 150 155 160
Ala Leu Gln Pro Gly Ser Lys Gln Val Ala Ala Gly Tyr Ile Val Tyr
165 170 175
Gly Ser Ser Thr Met Leu Val Tyr Thr Thr Gly Asn Gly Val Asn Gly
180 185 190
Phe Thr Leu Asp Pro Ser Ile Gly Glu Phe Ile Leu Ser His Pro Asn
195 200 205
Ile Arg Ile Pro Lys Thr Gly Ser Ile Tyr Ser Ile Asn Glu Gly Asn
210 215 220
Phe Asn Ser Phe Glu Glu Gly Leu Lys Arg Phe Ile Arg Trp Ala Gln
225 230 235 240
Glu Glu Asp Lys Ala Thr Gly Arg Pro Phe Ser Thr Arg Tyr Ile Gly
245 250 255
Ser Phe Val Ser Asp Phe His Arg Asn Leu Leu Lys Gly Gly Ile Tyr
260 265 270
Met Tyr Pro Ala Thr Lys Lys Asn Pro Glu Gly Lys Leu Arg Leu Met
275 280 285
Tyr Glu Ala Asn Pro Met Ala Phe Ile Val Glu Gln Ala Gly Gly Arg
290 295 300
Ala Ser Asp Gly His Arg Arg Ile Leu Asp Ile Val Pro Glu Lys Leu
305 310 315 320
His Gln Arg Thr Pro Leu Phe Ile Gly Ser Glu Glu Leu Val Arg Thr
325 330 335
Val Glu Glu Phe Leu Gln Gly Lys Arg
340 345
<210> 160
<211> 197
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 160
Met Lys Arg Arg Gly Leu Trp Ser Gly Thr Val Leu Ala Val Leu Ile
1 5 10 15
Thr Ala Ser Ala Gln Ala Gln Thr Ala Cys Asp Arg Pro Ala Leu Glu
20 25 30
Leu Arg Leu Leu Cys Thr Val Tyr Arg Trp Asp Gly Pro Val Ala Ser
35 40 45
Ala Tyr Phe Glu Thr Ile Asp Ala Thr Ala Tyr Pro Met Phe Ala Gly
50 55 60
Leu Thr Ala Met Ala Trp Gly Gly Val Leu Ala Gly Arg Leu Glu Arg
65 70 75 80
Pro Ala Ala Glu Arg Val Thr Leu Ala Thr Ala Ala Thr Thr Val Leu
85 90 95
Val Phe Gly Leu Lys Ala Leu Ala Arg Arg Asn Arg Pro Phe Asp Ala
100 105 110
Trp Asp Asp Ile Ala Pro Arg Gly Asp Pro Pro Thr Ser His Ala Phe
115 120 125
Pro Ser Gly His Ala Ala Leu Ala Phe Thr Leu Ala Thr Ala Trp Gly
130 135 140
Leu Glu Val Pro Arg Ile Tyr Val Ile Val Pro Ala Tyr Val Trp Ala
145 150 155 160
Thr Ser Val Ala Val Gly Arg Val Trp Lys Gly Val His Tyr Pro Thr
165 170 175
Asp Val Leu Ala Gly Ala Val Leu Gly Ala Gly Val Ala Trp Thr Val
180 185 190
His Arg Ile Trp Arg
195
<210> 161
<211> 269
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 161
Met Ser Trp Trp Glu Ala Leu Ile Leu Gly Leu Leu Gln Gly Leu Thr
1 5 10 15
Glu Phe Leu Pro Val Ser Ser Ser Gly His Leu Val Leu Gly Gln Tyr
20 25 30
Val Leu Gly Leu Asn Pro Gly Gly Val Thr Phe Glu Val Phe Val His
35 40 45
Phe Gly Thr Val Leu Ser Ile Leu Thr Val Tyr Arg Lys Arg Val Gly
50 55 60
Ala Ile Val Gly Glu Val Trp Thr Ala Leu Pro Arg Pro Ala Glu Trp
65 70 75 80
Pro Met Arg Tyr Arg Glu Arg Asp Pro Phe Arg Leu Ala Val Trp Ile
85 90 95
Leu Ile Thr Met Ile Pro Thr Gly Leu Val Tyr Val Leu Leu Gly Asp
100 105 110
Trp Ile Glu Gln Thr Phe Glu His Pro Arg Phe Ala Ala Gly Met Leu
115 120 125
Val Val Thr Gly Val Leu Leu Ile Leu Thr Arg Leu Arg Arg His Pro
130 135 140
Asp Gly Asp Leu Ser Pro Leu Lys Ala Phe Val Val Gly Val Ala Gln
145 150 155 160
Ser Ala Ala Met Leu Pro Gly Ile Ser Arg Ser Gly Ser Thr Ile Cys
165 170 175
Ala Ala Ile Tyr Gln Asn Val Arg Pro Glu Arg Ala Ala Asp Phe Ser
180 185 190
Phe Leu Met Leu Leu Pro Val Val Leu Gly Ala Thr Leu Leu Lys Gly
195 200 205
Ile Glu Leu Leu Asn Ser Pro Glu Ser Ile Gly Met Gly Pro Leu Val
210 215 220
Leu Gly Thr Val Ala Ala Tyr Val Ser Gly Val Ala Ala Ile Arg Met
225 230 235 240
Leu Leu Gln Val Val Arg Arg Gly Arg Leu Glu Tyr Phe Ala Tyr Tyr
245 250 255
Cys Phe Leu Val Gly Leu Leu Gly Leu Trp Leu Ile Arg
260 265
<210> 162
<211> 366
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 162
Met Ser Thr Arg Arg Ala Val Leu Asn Cys Phe Arg Arg His Gln Arg
1 5 10 15
Phe Val Leu Thr Thr His Ile Lys Pro Asp Gly Asp Ala Leu Gly Ser
20 25 30
Gln Leu Ala Leu Gly Arg Leu Leu Gln Lys Met Gly His Asp Val Tyr
35 40 45
Leu Ile Asn Ser Asp Pro Pro Pro Ser Asn Leu Thr Trp Ile Pro Gly
50 55 60
Ile Glu Gln Val Glu Val Phe Asn Gly Ala Leu Ala Gln Arg Glu Arg
65 70 75 80
Ile Asp Gln Ala Asp Val Ile Cys Val Leu Asp Thr Asn Ala Leu Asp
85 90 95
Arg Leu Gly Asp Leu Ala Pro Ala Val Glu Ala Ser Arg Ala Arg Lys
100 105 110
Leu Leu Ile Asp His His Thr Ser Pro Glu Asp Trp Phe Asp Leu Gln
115 120 125
Tyr Val Arg Asp Thr Ala Ser Ser Thr Gly Glu Leu Val Tyr Glu Leu
130 135 140
Val Cys Ala Val Asp Pro Asn Leu Ile Asp His Glu Leu Ala Thr Ala
145 150 155 160
Leu Tyr Val Ala Ile Met Thr Asp Thr Gly Ser Phe Arg Phe Asn Thr
165 170 175
Val Thr Pro Thr Val His Arg Ile Val Ala Asp Leu Leu Glu Arg Gly
180 185 190
Gly Leu Ser Thr Glu Ala Ile His Ser Ala Ile Phe Asp Thr Arg Thr
195 200 205
Pro Glu Ser Met Arg Leu Leu Gly Leu Ala Leu Arg Asn Leu Gln Leu
210 215 220
Arg Tyr Asp Gly Arg Val Ala Tyr Met Val Leu Ser Arg Arg Met Phe
225 230 235 240
Asn Glu Thr Gly Ala Ser Thr Glu Asp Thr Glu Gly Phe Ile Asn His
245 250 255
Leu Leu Ser Ile Arg Gly Val Arg Val Ala Leu Leu Phe Thr Glu Ile
260 265 270
Glu Lys Gly Val Lys Ile Ser Phe Arg Ser Lys Gly Asp Tyr His Val
275 280 285
Asn Glu Trp Ala Arg Ala Phe Gly Gly Gly Gly His Arg Asn Ala Ala
290 295 300
Gly Ala Phe Val Glu Asn Ala Ala Leu Asp Ala Leu Val Asp Ala Val
305 310 315 320
Leu Ala Ala Ala Pro Arg Tyr Leu Pro Gln Leu Glu Ala Ser Ser Ala
325 330 335
Gly Asn Ala Ser Gly Thr Leu Ser Ser Glu Asp Ala Ser Tyr Leu Ser
340 345 350
Ala Leu Leu His Gln Lys Ser Gln Ala Ser Ser Thr Ala Ser
355 360 365
<210> 163
<211> 174
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 163
Met Ser Gln Thr Asn His Gln Pro Ile Arg Val Leu Phe Val Cys Leu
1 5 10 15
Gly Asn Ile Cys Arg Ser Pro Leu Ala Glu Gly Val Phe Arg Lys Leu
20 25 30
Val Asp Glu Ala Gly Leu Thr Ala His Phe Glu Ile Asp Ser Ala Gly
35 40 45
Thr Gly Pro Trp His Val Gly Glu Pro Ala Asp Arg Arg Met Gln Arg
50 55 60
Thr Ala Arg Arg His Gly Val Asp Leu Ser Gly His Val Ala Arg Gln
65 70 75 80
Leu Gly Arg Glu Asp Leu Ala Arg Tyr Asp His Ile Tyr Val Met Asp
85 90 95
Arg Glu Asn Leu Glu Asp Val Leu Arg Leu Asp Arg Asp Gly Arg Phe
100 105 110
Arg His Lys Val Glu Leu Phe Arg Thr Phe Asp Pro Glu Pro Gly Asp
115 120 125
Gly Glu Val Pro Asp Pro Tyr Tyr Gly Gly Glu Arg Gly Phe Glu Glu
130 135 140
Val Tyr Gln Ile Val Glu Arg Thr Ala Arg Arg Leu Leu Glu His Leu
145 150 155 160
Val Ser Leu Tyr Lys Leu Lys Glu Thr Ala Asp Leu Ser Arg
165 170
<210> 164
<211> 272
<212> PRT
<213> 海洋红嗜热盐菌(Rhodothermus marinus)
<400> 164
Met Met Asp Ala Tyr Thr Leu Tyr Glu Glu Ala Arg Glu Val Ala Ala
1 5 10 15
Arg Leu Ala Arg Asp Ala Gly Gln Ile Ala Arg Tyr Tyr Ala Gly Arg
20 25 30
Val Thr Val Arg Glu Lys Gly Tyr Asn Glu Leu Val Thr Gln Ala Asp
35 40 45
Glu Glu Val Gln Arg Phe Leu Ile Glu Gln Ile His Arg His Phe Pro
50 55 60
Glu His Ala Ile Leu Ala Glu Glu Asn Leu Ser Asp Met Gln Asp Gly
65 70 75 80
Arg Glu Gly Ala Ser Phe Arg Trp Ile Ile Asp Pro Ile Asp Gly Thr
85 90 95
Thr Asn Phe Thr His Gly Val Pro Pro Tyr Gly Ile Ser Leu Ala Leu
100 105 110
Gln His Glu Gly Arg Thr Val Val Gly Val Val Tyr Asp Val Pro His
115 120 125
Asp Glu Leu Phe Thr Ala Val Arg Gly Gly Gly Leu Tyr Val Asn Gly
130 135 140
Val Arg Ala Arg Val Ser Gln Thr Glu Thr Leu Arg Glu Ala Leu Ile
145 150 155 160
Thr Thr Gly Phe Pro Tyr Arg Glu Val Val His Leu Glu Glu Tyr Leu
165 170 175
Glu Ala Leu Gly Arg Val Ile Arg Ala Thr Arg Gly Val Arg Arg Pro
180 185 190
Gly Ala Ala Ser Val Asp Leu Ala Trp Val Ala Cys Gly Arg Phe Asp
195 200 205
Gly Phe Phe Glu Thr Gly Leu Ser Pro Trp Asp Val Ala Ala Gly Ile
210 215 220
Leu Leu Val Glu Glu Gly Gly Gly Arg Val Thr Asp Phe His Gly Arg
225 230 235 240
Pro Asp Pro Ile Phe Ala Arg Gln Met Leu Ala Thr Asn Gly Arg Ile
245 250 255
His Glu Ala Leu Cys Glu Leu Val Ala Pro Leu His His Val Tyr Ala
260 265 270
<210> 165
<211> 219
<212> PRT
<213> Slackia heliotrinireducens
<400> 165
Met Ile Leu Lys Ala Ala Leu Phe Asp Asn Asp Gly Thr Leu Val Asp
1 5 10 15
Ser Glu Glu Leu Ile Leu Ser Ser Phe Arg Tyr Ala Thr Lys Ser Val
20 25 30
Leu Gly Glu Ala Leu Pro Asp Glu Val Leu Arg Arg Lys Val Gly Gln
35 40 45
Pro Leu Arg Thr Gln Met Ala Asp Phe Thr Pro Asp Val Asp Lys Arg
50 55 60
Glu Glu Leu Phe Arg Val Tyr Gln Glu Phe Asn Ala Arg Glu His Asp
65 70 75 80
Arg Met Ile Arg Leu Phe Pro Asp Val Ala Asn Thr Leu Gly Thr Met
85 90 95
Leu Gln Arg Gly Leu Arg Leu Gly Val Val Thr Ser Lys Leu Ser Glu
100 105 110
Asn Cys Leu Gln Asn Leu Ser His Leu Gly Ile Asp Gly Tyr Phe Glu
115 120 125
Cys Ile Val Ala Pro Asp Asn Cys Pro Leu His Lys Pro Asp Pro Gly
130 135 140
Pro Val Leu Glu Gly Ala Lys Leu Leu Gly Ala Arg Pro Glu Gln Cys
145 150 155 160
Val Tyr Val Gly Asp Ser Pro Tyr Asp Ile Ala Ala Gly Arg Asp Ala
165 170 175
Gly Cys Thr Thr Ile Ala Val Thr Tyr Gly Val Phe Ser Arg Glu Asp
180 185 190
Leu Lys Pro Glu Arg Pro Asp Tyr Phe Cys Asp Ser Phe Ala Glu Leu
195 200 205
Leu Ser Val Leu Asp Gly Ile Val Arg Arg Gly
210 215
<210> 166
<211> 264
<212> PRT
<213> Slackia heliotrinireducens
<400> 166
Met Leu Ala His Gln Asn Glu Thr Met Thr Lys Tyr Leu Phe Phe Asp
1 5 10 15
Ile Asp Gly Thr Leu Ala Ala Gly Pro Ile Gly Asn Arg Val Val Pro
20 25 30
Glu Gly Thr Arg Asp Ala Leu Leu Arg Leu Arg Lys Asn Gly His Phe
35 40 45
Thr Ala Ile Cys Thr Gly Arg Ser His Ala Met Ala Arg Ser Tyr Met
50 55 60
Glu Glu Gln Gly Phe Thr Asn Met Val Ala Asp Gly Gly Asn Ser Thr
65 70 75 80
Val Ile Asp Gly Asn Leu Leu Gly Ile Glu Pro Leu Asp Arg Lys Arg
85 90 95
Cys Ile Gln Val Leu Glu Glu Cys Asp Arg Leu Lys Ile Pro Trp Ala
100 105 110
Val Ser Ala Asp Asp Ser Asn Thr Arg Leu Ser Ile Asp Gly Arg Phe
115 120 125
Ala Asp Leu Val Glu Lys Gly Tyr Met Val Thr Lys Thr Val Pro Asn
130 135 140
Leu Asp Tyr His Ala Ile Pro Arg Phe Tyr Lys Leu Tyr Ile Ala Val
145 150 155 160
Asn Ala Glu Asp Glu Lys Arg Ile Thr Thr Leu Asp Leu Val Pro His
165 170 175
Val His Phe Ser Asp Thr Cys Leu Phe Val Glu Pro Ile Asp Lys Ser
180 185 190
Arg Gly Ile Met Arg Ile Met Glu His Leu Gly Ala Pro Ile Glu Asp
195 200 205
Val Val Val Phe Gly Asp Gly Met Asn Asp Leu Ser Met Phe Asp Pro
210 215 220
Arg Trp Leu Ser Ile Ala Met Gly Asn Ala Val Gln Pro Leu Lys Asp
225 230 235 240
Ala Ala Asp Tyr Ile Thr Ala Asn Ala Asp Asp Asp Gly Ile Arg Lys
245 250 255
Ala Cys Glu His Phe Gly Trp Ile
260
<210> 167
<211> 238
<212> PRT
<213> 海葡萄嗜热菌(Staphylothermus marinus)
<400> 167
Met Ile Arg Leu Ala Ala Phe Asp Ile Asp Gly Thr Leu Thr Ile Asn
1 5 10 15
Arg Ser Ser Thr Val Leu Cys Leu Glu Ala Ile Asp Ala Leu Arg Lys
20 25 30
Leu Glu Lys Asn Gly Val Ile Val Val Leu Val Ser Ser Asn Ala Leu
35 40 45
Pro Val Val Val Gly Leu Lys Lys Tyr Ile Gly Leu Ser Gly Pro Ala
50 55 60
Ile Gly Glu Thr Gly Ala Leu Ile Tyr Tyr Gly Glu Glu Glu Ile Val
65 70 75 80
Ala Thr Thr Lys Tyr Ser Ala Lys Gln Ala Tyr Leu Asp Val Leu Glu
85 90 95
Lys Tyr Asn Glu Tyr Val Tyr Gly Ser Trp Gln Asn Met Phe Arg Leu
100 105 110
His Asp Tyr Ala Leu Lys Ile Arg Lys Gln Tyr Leu Ser Lys Asp Asn
115 120 125
Glu Ile Tyr Ser Leu Ile Lys Glu Tyr Val Glu Asn Lys Tyr Pro Tyr
130 135 140
Ile Lys Val Gly Tyr Ser Gly Tyr Ala Ile His Leu Thr Pro Lys Asp
145 150 155 160
Thr Gly Lys Gly Lys Ala Leu Lys Gln Ile Met Glu Lys His Gly Ile
165 170 175
Arg Arg Glu Glu Thr Met Gly Val Gly Asp Ser Ile Met Asp Trp Glu
180 185 190
Phe Ile Lys Glu Thr Lys Ile Lys Val Ala Val Ala Asn Ala Asp Pro
195 200 205
Glu Leu Arg Arg Lys Ala Asp Ile Val Thr Thr Lys Pro Ser Gly Tyr
210 215 220
Gly Val Val Glu Ile Val Glu Lys Ile Leu Asp Lys Pro Pro
225 230 235
<210> 168
<211> 227
<212> PRT
<213> 海葡萄嗜热菌(Staphylothermus marinus)
<400> 168
Met Ala Arg Ile Lys Ala Val Leu Leu Asp Tyr Asp Leu Thr Leu Met
1 5 10 15
Asn Asn Leu Ile Asp Phe Tyr Asp Ala Tyr Asn Glu Ala Leu Lys Lys
20 25 30
Phe Val Gly Lys Thr Leu Gly Phe Asn Glu Phe Phe His Leu Leu Ile
35 40 45
Asn Tyr Ser Leu Gln Ala Tyr Ile Pro Pro Asp Val Asp Arg Phe Ser
50 55 60
Phe Trp Arg Tyr Phe Arg Gln Val Tyr Arg Thr Arg Tyr Gly Tyr Pro
65 70 75 80
Met Glu Gly Ala Tyr Tyr Phe Leu Tyr Trp Val Lys Ala Leu Gly Leu
85 90 95
Lys Thr Ile Ile Val Ser Gly Arg Glu Cys His Glu Ser Ser Ile Trp
100 105 110
Glu Glu Leu Lys Arg Phe Gly Leu Asn Glu Tyr Ile Asp Lys Val Tyr
115 120 125
Thr Met Phe Asp Thr Leu Ile Leu Gly Gly Val Glu Glu Glu Leu Phe
130 135 140
Asp Lys Thr Trp Leu Ile Ser Tyr Ala Leu Ser Ser Tyr Gly Leu Asp
145 150 155 160
Arg Asp Glu Val Val Tyr Ile Gly Asp Tyr Arg Gln Asp Leu Leu Ser
165 170 175
Ser Gln Arg Thr Gly Ile Lys Phe Ile Gly Ile Ala Phe Ser Glu Lys
180 185 190
Arg Lys Glu Cys Leu Arg Arg Leu Gly Ala Glu Tyr Val Gly Ser Asn
195 200 205
Leu Tyr Asp Val Thr Tyr Tyr Leu Trp Gln Ile Ile Arg Glu Val Glu
210 215 220
Phe Lys Lys
225
<210> 169
<211> 222
<212> PRT
<213> 海葡萄嗜热菌(Staphylothermus marinus)
<400> 169
Met Thr Ser Thr Glu Val Val Lys Ala Val Leu Phe Asp Met Asp Gly
1 5 10 15
Thr Ile Ile Asn Ser Val Glu Leu Ile Ala Glu Cys Trp Ser Lys Ala
20 25 30
Phe Lys Lys His Gly Ile Arg Ile Glu Pro Gln Asp Ile Tyr Arg Val
35 40 45
Val Gly Leu Pro Ala Asp Thr Ile Leu Glu Lys Tyr Thr Gly Thr Lys
50 55 60
Asn Pro Arg Leu His Asn Ser Ile Leu Glu Gln Ala Arg Lys Cys Phe
65 70 75 80
Glu Glu Lys Met Asn Pro Asn Thr Leu Leu Tyr Asn Asp Val Leu Glu
85 90 95
Thr Ile Lys Gln Leu Arg Glu Asn Asn Lys Leu Cys Gly Ile Val Thr
100 105 110
Ser Ser Ser Cys Lys Arg Thr Ile Glu Leu Leu Glu Lys Leu Asp Ile
115 120 125
Ile Glu Tyr Phe Asp Thr Ile Gln Cys Tyr Gln Gly Lys Leu Arg Gly
130 135 140
Lys Pro Tyr Pro Asp Leu Leu Leu Ser Ala Leu Asn Lys Leu Gly Ile
145 150 155 160
Lys Pro Ser Gln Ala Ile Tyr Val Gly Asp Ser Tyr Ile Asp Tyr Leu
165 170 175
Thr Ala Arg Asn Thr Gly Val Leu Phe Val Leu Val Lys Arg Ser Trp
180 185 190
Asn Thr His Ile Val Asp Lys Cys Ser Glu Lys Cys Ile Val Ile Ser
195 200 205
Asp Leu Arg Glu Ile Ser Asn Leu Ile Thr Asn Lys Ile Lys
210 215 220
<210> 170
<211> 225
<212> PRT
<213> 嗜酸热硫化叶菌(Sulfolobus acidocaldarius)
<400> 170
Met Asn Lys Asp Ile Ile Phe Val Ser Asp Tyr Asp Arg Thr Leu Ser
1 5 10 15
Ser Glu Lys Asn Asn Phe Arg Ile Asp Lys Glu Val Ala Arg Ile Val
20 25 30
Asn Asp Phe Ser Lys Thr Tyr Pro Phe Phe Val Val Thr Gly Arg Glu
35 40 45
Lys Lys Phe Ile Asp Ile Leu Ala Pro Glu Leu Lys Pro Thr Gly Trp
50 55 60
Ile Leu Glu Asn Gly Ala Leu Met Tyr Val Asn Gly Glu Leu Ile Tyr
65 70 75 80
Asn Ile Gln Pro Ser Trp Phe Asp Thr Arg Lys Asn Ile Ile Lys Ile
85 90 95
Leu Asp Asn Tyr Asn Ile Ser Tyr Ser Leu Gly Asn Val Ile Val Tyr
100 105 110
Val Asp Lys Ala His Glu Tyr Lys Gly Val Leu Asn Ser Ile Lys Asp
115 120 125
Ala Thr Val Glu Trp Asn Arg Asn Asp Ala Met Ile Leu Pro Lys Gly
130 135 140
Val Asp Lys Gly Ser Ala Val Ile Gln Leu Arg Glu Arg Leu Gly Tyr
145 150 155 160
Arg Gly Lys Ile Val Ala Ile Gly Asp Ser Glu Asn Asp Ile Ser Met
165 170 175
Phe Arg Val Ala Asp Ile Arg Val Ser Val Ala Asn Ala Leu Pro Met
180 185 190
Ile Lys Glu Ile Ser Glu Leu Ile Leu Glu Lys Glu Asp Gly Glu Gly
195 200 205
Val Lys Glu Phe Leu Leu Lys Val Leu Lys Gly Glu Ile Ile Leu Ile
210 215 220
Lys
225
<210> 171
<211> 224
<212> PRT
<213> 嗜酸热硫化叶菌(Sulfolobus acidocaldarius)
<400> 171
Met Ile Tyr Asn Ile Phe Ile Phe Val Ile Lys Tyr Asn Phe Pro Val
1 5 10 15
Phe Lys Val Lys Gly Val Ile Phe Asp Leu Asp Gly Thr Leu Ala Asn
20 25 30
Thr Ala Leu Ile His Lys Glu Ala Trp Glu Lys Ala Leu Asp Arg Leu
35 40 45
Gly Ile Lys Ser Asp Val Lys Ile Asp Asn Leu Leu Gly Arg Lys Ser
50 55 60
Ser Asp Ile Ala Lys Leu Leu Ala Pro Asn Asn Trp Ser Glu Leu Ile
65 70 75 80
Asn Ile Lys Asn Gln Ile Tyr Val Glu Leu Val Lys Glu Lys Ala Ser
85 90 95
Pro Thr Pro Cys Ala Leu Asp Leu Leu Ser Tyr Leu Arg Lys Lys Glu
100 105 110
Ile Lys Thr Ala Ile Val Thr Ser Ser Asn Lys Leu Ser Ser Gly Ser
115 120 125
Val Leu Lys Lys Leu Gly Ile Thr Ser Glu Val Val Leu Thr Gly Asp
130 135 140
Asp Val Ile Asn Ser Lys Pro Asn Pro Glu Gly Ile Asn Lys Ala Leu
145 150 155 160
Ser Leu Leu Tyr Leu Asp Gly Arg Asp Val Ile Gly Val Gly Asp Thr
165 170 175
Glu Val Asp Val Glu Ala Tyr Tyr Lys Ala Gly Leu Gly Gly Ile Tyr
180 185 190
Leu Val Lys Ser Gly Val Pro Phe Arg Glu Glu Val Val Lys Met Tyr
195 200 205
Gly Gly Ile Val Ile Ser Ser Leu Cys Glu Leu Leu Glu Leu Ile Ser
210 215 220
<210> 172
<211> 267
<212> PRT
<213> 嗜酸热硫化叶菌(Sulfolobus acidocaldarius)
<400> 172
Met Lys Arg Glu Asp Val Glu Lys Val Ala Ser Glu Ala Ser Lys Tyr
1 5 10 15
Ile Tyr Glu Glu Arg Glu Asn Lys Asp Val Asp Arg Val Ile Asn Val
20 25 30
His Gly Asn Asp Val Thr Arg Ile Ile Asp Lys Arg Ser Glu Asp Phe
35 40 45
Ile Val Asp Arg Leu Lys Ser Leu Gly Tyr Asn Ile Leu Ile Val Thr
50 55 60
Glu Glu Ser Gly Val Ile Asp Ser Tyr Gly Lys Asn Tyr Asp Tyr Ile
65 70 75 80
Ala Ile Val Asp Pro Leu Asp Gly Ser Thr Asn Phe Val Ser Gly Ile
85 90 95
Pro Trp Ser Ser Val Ser Ile Ala Ile Tyr Asn Arg Asp Glu Glu Asp
100 105 110
Ile Leu Ser Ser Asn Val Gly Ala Val Ser Ser Ile Phe Thr Pro Tyr
115 120 125
Thr Phe Ser Tyr Asp Glu Gly Ser Ala Tyr Val Asn Gly Val Lys Ile
130 135 140
Ala Glu Ile Lys Lys Pro Glu Lys Ile Leu Leu Leu Ala Tyr Phe Ser
145 150 155 160
Arg Ser Lys Leu Pro Asn Leu Lys Leu Phe Phe Glu Lys Ile Gly Gln
165 170 175
Gly Tyr Lys Ile Arg Ser Leu Gly Ser Ala Ser Leu Asp Met Ile Leu
180 185 190
Val Cys Thr Gly Arg Ala Thr Met Phe Phe Asp Ile Arg Gly Lys Leu
195 200 205
Arg Asn Val Asp Ile Ala Ala Ser Ser Asn Phe Cys Ser Arg Leu Gly
210 215 220
Val Ile Pro Tyr Asp Ile Gly Leu Arg Lys Ile Lys Ser Ser Leu Thr
225 230 235 240
Glu Val Ser Val Val Lys Asp Leu Val Ile Ser Leu Asp Glu Ser Leu
245 250 255
Leu Arg Ser Phe Ser Leu Ala Leu Gln Thr Val
260 265
<210> 173
<211> 272
<212> PRT
<213> Thermanaerothrix daxensis
<400> 173
Met Thr Thr Thr Pro Ile Val Leu Ser Ile Asp Leu Asp Gly Thr Leu
1 5 10 15
Leu Ile Glu Glu Arg Glu Val His Pro Glu Asp Val His Leu Leu His
20 25 30
His Pro Leu Pro Val Thr Leu Ile Leu Ala Thr Gly Arg Ser Leu Glu
35 40 45
Ser Ala Arg Arg Val Leu Glu Arg Ala Gly Leu Tyr Arg His Glu Pro
50 55 60
Leu Pro Phe Pro Leu Val Leu Gln Asn Gly Ala Ala Ile Tyr Arg Pro
65 70 75 80
Gln Glu Val Leu Glu Asp Tyr Phe Pro Ile Pro Pro Glu Ala Glu Thr
85 90 95
Gln Leu His Ala Leu Ala Pro Gln Phe Ser Glu Ala Ala Leu Leu Trp
100 105 110
Gln Gly Leu Asp Gly Ala Tyr Leu Glu Glu Ile Thr Pro Phe Gly Leu
115 120 125
Gln Ala Ala Gly Arg Phe Gly Phe Leu Pro Arg Pro Leu Ser Glu Ala
130 135 140
Pro Ala Gly Gln Gly Phe Gly Lys Leu Met Cys Leu Ser Asp Arg Pro
145 150 155 160
Glu Val Leu Lys Ala Phe Ala Ala Ala Thr Arg His Leu Pro Val Glu
165 170 175
Gly Ser Tyr Ser Leu Asp Val Ile Tyr Glu Val Thr Arg Gln Gly Val
180 185 190
His Lys Gly Phe Gly Leu Arg Ile Leu Leu Gln Arg Leu Gly Leu Thr
195 200 205
His Ala Arg Leu Val Ala Val Gly Asp Gly Asp Asn Asp Val Pro Met
210 215 220
Phe Ser Cys Ala Asp Leu Ala Ile Ala Pro Leu Asn Ala Ser Pro Leu
225 230 235 240
Ala Arg Ser His Ala His Arg Leu Ile Asp Arg Arg Pro His Gly Leu
245 250 255
Leu Thr Pro Val Leu Asp Leu Leu Arg Gln Glu Gly Trp Leu Asp Pro
260 265 270
<210> 174
<211> 215
<212> PRT
<213> 嗜热厌氧菌(Thermoanaerobacter sp.)
<400> 174
Met Asn Val Lys Ala Val Val Phe Asp Leu Asp Gly Thr Leu Ile Asp
1 5 10 15
Ser Lys Lys Asp Ile Ala Met Ala Ala Asn Lys Thr Leu Ser Glu Leu
20 25 30
Gly Leu His Thr Leu Pro Glu Asp Thr Ile Ala Ser Phe Ile Gly Tyr
35 40 45
Gly Gly Glu Leu Phe Ile Lys Arg Cys Ile Gly Glu Lys Asn Ile Asp
50 55 60
Lys Phe Glu Glu Ala Phe Lys Lys Phe Lys Glu Asn Tyr Ser Glu Leu
65 70 75 80
Cys Ile Ile His Thr Ser Leu Phe Pro Gly Val Glu Glu Val Leu Glu
85 90 95
Phe Leu Lys Lys Arg Lys Ile Asn Ile Ala Leu Ala Thr Asn Lys Met
100 105 110
Ile Ser Leu Ser Lys Lys Ile Leu Lys His Leu Glu Val Glu Lys Tyr
115 120 125
Phe Ser Ile Met Leu Gly Pro Glu Asp Val Thr Asn Arg Lys Pro His
130 135 140
Pro Glu Ile Ile Glu Ile Leu Leu Gln Asn Leu Asn Val Arg Pro Glu
145 150 155 160
Glu Thr Leu Tyr Val Gly Asp Ser Glu Ile Asp Val Phe Cys Gly Lys
165 170 175
Ser Ala Gly Val Tyr Thr Cys Ala Val Thr Tyr Gly Ile Gly Asp Ile
180 185 190
Lys Ser Ile Ile Ala Ala Asp Pro Asp Phe Ile Ile Thr Asp Leu Thr
195 200 205
Lys Leu Ile Ile Leu Leu Ser
210 215
<210> 175
<211> 273
<212> PRT
<213> 热硫化氢嗜热厌氧杆菌(Thermoanaerobacter thermohydrosulfuricus)
<400> 175
Met Tyr Lys Leu Phe Val Thr Asp Ala Asp Gly Ser Leu Leu Asn Ser
1 5 10 15
Asn Ser Glu Ile Ser Asp Lys Asn Lys Glu Ala Ile Lys Glu Val Ile
20 25 30
Ser Arg Gly Val Ile Phe Thr Ile Ala Thr Gly Arg Met Phe Ser Ser
35 40 45
Ile Leu Pro Tyr Ala Phe Glu Leu Asn Val Asn Ala Pro Val Ile Ser
50 55 60
Tyr Asn Gly Ala Leu Ile Lys Asp Ile Tyr Thr Lys Lys Val Tyr Tyr
65 70 75 80
Tyr Asn Pro Ile Gln Thr Glu Asp Ala Ile Phe Ala Ile Arg Leu Leu
85 90 95
Lys Glu Ser Gly Tyr His Ile Asn Leu Tyr Ile Asp Asp Glu Leu Tyr
100 105 110
Val Glu Glu Ile Thr Asp Arg Val Glu Trp Tyr Leu Ser Phe Asn Asn
115 120 125
Val Thr Val Asn Ala Val Gly Asn Leu Glu Glu Phe Leu Lys Arg Thr
130 135 140
Gly Gly Val Thr Ala Lys Ile Tyr Ala Ile Asn Asp Met Lys Asn Pro
145 150 155 160
Ile Ser Ile Asp Ala Glu Ile Tyr Asp Glu Ile Ser Lys Arg Leu Thr
165 170 175
Ile Ser Thr Ser Gly Gly Gly His Leu Glu Ile Asn Ala Lys Gly Val
180 185 190
Ser Lys Gly Asn Ala Leu Lys Thr Leu Ala Asn Met Tyr Ser Ile Lys
195 200 205
Arg Glu Gln Val Val Ala Ile Gly Asp Asn Leu Asn Asp Leu Ser Met
210 215 220
Ile Glu Tyr Ala Gly Leu Gly Val Ala Met Gly Asn Ala Pro Asp Ile
225 230 235 240
Val Lys Ile Lys Ala Asp Tyr Thr Thr Leu Ser Asn Asp Glu Asp Gly
245 250 255
Val Ala His Val Ile Asp Lys Phe Phe Leu Asn Lys Lys Thr Val Ala
260 265 270
Val
<210> 176
<211> 273
<212> PRT
<213> 热硫化氢嗜热厌氧杆菌(Thermoanaerobacter thermohydrosulfuricus)
<400> 176
Met Tyr Lys Leu Phe Val Thr Asp Ala Asp Gly Ser Leu Leu Asn Ser
1 5 10 15
Asn Ser Glu Ile Ser Asp Lys Asn Lys Glu Glu Ile Lys Glu Val Ile
20 25 30
Ser Arg Gly Val Ile Phe Thr Ile Ala Thr Gly Arg Met Phe Ser Ser
35 40 45
Ile Leu Pro Tyr Ala Phe Glu Leu Asn Val Asn Ala Pro Val Ile Ser
50 55 60
Tyr Asn Gly Ala Leu Ile Lys Asp Ile Tyr Thr Lys Lys Val Tyr Tyr
65 70 75 80
Tyr Asn Pro Ile Gln Thr Glu Asp Ala Ile Phe Ala Ile Arg Leu Leu
85 90 95
Lys Glu Ser Gly Tyr His Ile Asn Leu Tyr Ile Asp Asp Glu Leu Tyr
100 105 110
Val Glu Glu Ile Thr Asp Arg Val Glu Trp Tyr Leu Ser Phe Asn Asn
115 120 125
Val Thr Val Asn Ala Val Gly Asn Leu Glu Glu Phe Leu Lys Arg Thr
130 135 140
Gly Gly Val Thr Ala Lys Ile Tyr Ala Ile Asn Asp Met Lys Asn Pro
145 150 155 160
Ile Ser Ile Asp Ala Glu Ile Tyr Asp Glu Ile Ser Lys Arg Leu Thr
165 170 175
Ile Ser Thr Ser Gly Gly Gly His Leu Glu Ile Asn Ala Lys Gly Val
180 185 190
Ser Lys Gly Asn Ala Leu Lys Thr Leu Ala Asn Met Tyr Ser Ile Lys
195 200 205
Arg Glu Gln Val Val Ala Ile Gly Asp Asn Leu Asn Asp Leu Ser Met
210 215 220
Ile Glu Tyr Ala Gly Leu Gly Val Ala Met Gly Asn Ala Pro Asp Ile
225 230 235 240
Val Lys Ile Lys Ala Asp Tyr Thr Thr Leu Ser Asn Asp Glu Asp Gly
245 250 255
Val Ala His Val Ile Asp Lys Phe Phe Leu Asn Lys Lys Thr Val Ala
260 265 270
Val
<210> 177
<211> 257
<212> PRT
<213> 威吉利热厌氧杆菌(Thermoanaerobacter wiegelii)
<400> 177
Met Lys Asp Glu Lys Gly Ile Val Val Asp Ile Ile Lys Lys Ala Gly
1 5 10 15
Glu Ile Leu Gln Asp Gly Trp Asn Lys Lys Asn Phe Lys Ile Tyr Arg
20 25 30
Lys Gly Thr Ile Asn Leu Val Thr Glu Ile Asp Lys Lys Ile Glu Phe
35 40 45
Leu Ile Ile Gln Leu Leu Lys Gln Tyr Phe Pro Asp Tyr Gly Ile Leu
50 55 60
Thr Glu Glu Ser Lys Glu Ile Asn Ser Lys Ala Asn Val Arg Trp Ile
65 70 75 80
Ile Asp Pro Leu Asp Gly Thr Thr Asn Tyr Ile Lys Gln Tyr Pro Phe
85 90 95
Val Ala Ile Ser Ile Ala Leu Glu Lys Glu Gly Glu Leu Ile Leu Gly
100 105 110
Val Val Tyr Asn Pro Ile Leu Asn Glu Met Phe Ile Ala Gln Lys Gly
115 120 125
Cys Gly Ala Thr Tyr Asn Gly Lys Ser Ile His Val Ser Lys Ile Lys
130 135 140
Glu Leu Gly Ser Ala Val Leu Ala Ser Gly Phe Pro Tyr Asp Ala Trp
145 150 155 160
Glu Asn Pro Asp Asn Asn Ala Lys Gln Trp Arg Gln Phe Leu Thr Arg
165 170 175
Ser Leu Ser Leu Arg Cys Asp Gly Ser Ala Ala Leu Asp Leu Cys Arg
180 185 190
Val Ala Cys Gly Gln Leu Asp Gly Tyr Trp Glu Lys Gly Ile Ser Pro
195 200 205
Trp Asp Val Ala Ala Gly Ile Val Ile Leu Arg Glu Ala Gly Gly Ile
210 215 220
Ile Thr Asp Tyr Leu Gly Glu Glu Asn Phe Phe Lys Arg Gly Glu Val
225 230 235 240
Val Ala Ala Asn Pro Val Leu His Ala Gln Met Leu Lys Val Leu Asn
245 250 255
Asn
<210> 178
<211> 215
<212> PRT
<213> 威吉利热厌氧杆菌(Thermoanaerobacter wiegelii)
<400> 178
Met Asn Val Lys Thr Ile Ile Phe Asp Leu Asp Gly Thr Leu Ile Asp
1 5 10 15
Ser Lys Lys Asp Ile Val Met Ala Ile Asn Lys Thr Leu Arg Asp Leu
20 25 30
Asp Ile Pro Thr Leu Pro Glu Asp Asp Ile Ile Pro Phe Met Ser Tyr
35 40 45
Gly Pro Glu Val Phe Ile Lys Gln Cys Ile Gly Glu Lys Asn Ala Asp
50 55 60
Lys Phe Glu Arg Ala Phe Glu Lys Phe Lys Glu Asn Tyr Ser Glu Arg
65 70 75 80
Cys Ile Val Tyr Thr Ser Leu Phe Pro Gly Val Arg Glu Val Leu Glu
85 90 95
Phe Leu Lys Glu Arg Lys Ile Asn Ile Ala Leu Ala Thr Asn Lys Met
100 105 110
Met Ser Leu Ser Lys Lys Ile Leu Gln His Phe Gly Leu Glu Lys Tyr
115 120 125
Phe Ser Ile Met Leu Gly Pro Glu Asp Val Thr Asn Lys Lys Pro His
130 135 140
Pro Glu Ile Ile Glu Ile Ile Leu Gln Lys Leu Asn Val Lys Arg Glu
145 150 155 160
Glu Ala Leu Tyr Val Gly Asp Ser Glu Ile Asp Val Leu Cys Gly Lys
165 170 175
Ser Ala Gly Val Tyr Thr Cys Ala Val Thr Tyr Gly Ile Gly Asp Ile
180 185 190
Lys Ser Ile Ile Ala Ala Asn Pro Asp Phe Ile Ile Thr Asp Leu Thr
195 200 205
Lys Leu Ile Leu Leu Val Ser
210 215
<210> 179
<211> 205
<212> PRT
<213> 木聚糖热厌氧杆菌(Thermoanaerobacterium xylanolyticum)
<400> 179
Met Ser Thr Arg Leu Phe Ile Val Arg His Gly Glu Thr Leu Trp Asn
1 5 10 15
Arg Gln Lys Lys Ile Gln Gly Ala Ser Asp Thr Gln Leu Ser Asp Glu
20 25 30
Gly Met Lys Gln Ala Tyr Leu Leu Ser Gln Arg Leu Lys Asn Glu Ile
35 40 45
Ile Asp Val Ile Phe Ser Ser Asp Leu Asp Arg Ala Tyr Lys Thr Ala
50 55 60
Thr Phe Ile Ala Lys Asn Phe Asn Leu Asp Val Ile Lys Leu Pro Glu
65 70 75 80
Leu Arg Glu Ile Ser Phe Gly Val Trp Glu Gly Leu Thr Val Asp Glu
85 90 95
Ile Glu Lys Ser Tyr Lys Glu Leu Tyr His Thr Trp Lys Thr Asn Pro
100 105 110
Pro Glu Ala Thr Ile Glu Gly Ala Glu Thr Leu Lys Ala Val Gln Asp
115 120 125
Arg Ile Leu Asn Ala Thr Asn Lys Ile Ile Glu Gln Tyr Lys Asn Lys
130 135 140
Asn Ile Leu Ile Val Ser His Gly Thr Thr Ile Lys Ala Leu Ile Leu
145 150 155 160
Gly Met Leu Asn Leu Asp Leu Ser Phe Tyr Pro Lys Ile Arg Gln Asp
165 170 175
Asn Thr Ala Leu Asn Ile Ile Asp Val Lys Asp Asp Gly Asn Cys Val
180 185 190
Leu Val Leu Leu Asn Asp Thr Cys His Leu Arg Glu Arg
195 200 205
<210> 180
<211> 595
<212> PRT
<213> 耐盐嗜热双岐菌(Thermobifida halotolerans)
<400> 180
Met Arg Tyr His Val Leu Ala Cys Asp Tyr Asp Glu Thr Ile Ala His
1 5 10 15
Gly Gly Arg Val Asp Asp Ala Thr Val Thr Ala Leu Glu His Leu Ser
20 25 30
Arg Ser Gly Arg Arg Leu Val Met Val Thr Gly Arg Arg Leu Asp Asp
35 40 45
Leu Phe Gly Val Phe Asp His Val Asp Leu Phe Asp Met Val Val Ala
50 55 60
Glu Asn Gly Gly Val Leu Tyr Asp Pro Gly Arg Arg Asp Arg Arg Pro
65 70 75 80
Leu Ala Glu Arg Pro Pro Ala Glu Phe Val Glu Arg Leu Arg Arg His
85 90 95
Gly Val Ser Pro Leu Ala Val Gly Glu Val Val Val Ala Thr Arg Glu
100 105 110
Pro His Asp Arg Thr Val Leu Asp Ala Ile Arg Glu Leu Gly Leu Glu
115 120 125
Leu Gln Val Ile Tyr Asn Lys Gly Ala Val Met Val Leu Pro Pro Gly
130 135 140
Val Asn Lys Ala Ser Gly Leu Ala Ala Ala Leu Arg Arg Leu Glu Phe
145 150 155 160
Ser Pro His Ser Thr Val Ala Val Gly Asp Ala Glu Asn Asp His Ala
165 170 175
Met Leu His Met Cys Glu Cys Ala Val Ala Val Ala Gly Ala Leu Glu
180 185 190
Ala Val Lys Arg Thr Ser Asp Leu Val Leu Glu Arg Pro Asp Gly Glu
195 200 205
Gly Val Ala Glu Leu Val Asp Arg Leu Leu Ala Thr Asp Leu Ala Glu
210 215 220
Val Pro Val Ser Arg His Asp Leu His Phe Gly Thr Ala Gly Ala Ala
225 230 235 240
Pro Thr Ala Ile Ala Pro His Gly Arg Gly Gly Ala Val Ala Gly Pro
245 250 255
Ser Gly Ser Gly Lys Ser Thr Ala Ala Thr Ala Leu Leu Glu Arg Leu
260 265 270
Gly Glu Ser Gly Tyr Gln Tyr Cys Val Ile Asp Pro Glu Gly Asp Tyr
275 280 285
Ala Asp Phe Glu Gly Val Thr Ala Phe Gly Asp Ala Thr Arg Ala Pro
290 295 300
Ser Ile Asp Glu Val Leu Thr Ala Leu Arg Asp Pro Asp His Asn Val
305 310 315 320
Ala Val Asn Met Leu Ala Val Pro Leu Arg Asp Arg Pro Ala Phe Phe
325 330 335
Ala Gly Leu Leu Pro Arg Leu Val Gly Leu Arg Ala Glu Leu Gly His
340 345 350
Pro Ala Trp Leu Val Val Asp Glu Ala His His Leu Met Pro Val Glu
355 360 365
Leu Ala Gln Leu Pro Leu Arg Val Pro Ala Asp Val Gly Gly Leu Met
370 375 380
Leu Val Thr Val His Pro Glu Thr Leu Ser Pro Leu Val Leu Arg Leu
385 390 395 400
Val Asp Thr Val Val Ala Val Gly Asp Asp Pro Gly Gln Thr Leu Asp
405 410 415
Ala Phe Ala Ala Ala Thr Gly His Arg Pro Pro Ala Leu Glu Pro Ala
420 425 430
Pro Ala Glu Pro Asp Arg Gln Gln Glu Ser Val Val Ser Asp Ile Ala
435 440 445
Val Trp Arg Val Ala Glu Thr Ala Ala Gln Arg Val Lys Leu Ala Pro
450 455 460
Gly Glu Thr His Arg Thr Arg His Arg Arg Lys Tyr Ala Ala Gly Thr
465 470 475 480
Leu Ala Pro Asp Lys Ser Phe Tyr Phe Thr Gly Pro Gln Gly Arg Leu
485 490 495
Arg Leu Arg Ala Arg Asn Leu His Ser Phe Val Glu Leu Ala Glu Gly
500 505 510
Val Asp Asp Asp Thr Trp Thr His His Leu Arg Arg His Asp Tyr Ser
515 520 525
Arg Trp Leu Arg Gly Ser Val Arg Asp Gly Glu Leu Ala Asp Leu Val
530 535 540
Arg Arg Val Glu Gln Asp Thr Gly Arg Ala Pro Ser Asp Ser Arg Arg
545 550 555 560
Glu Val Ala Arg Leu Ile Asp Glu Arg Tyr Thr Leu Pro Ala Glu Pro
565 570 575
Thr Ser Tyr Asp Pro Asp Arg Gly Arg Glu Thr Gly Pro Ser Gly Arg
580 585 590
Arg Arg Pro
595
<210> 181
<211> 237
<212> PRT
<213> 耐盐嗜热双岐菌(Thermobifida halotolerans)
<400> 181
Met Gly Leu Arg Glu Thr Asp Thr Glu Phe Val Pro Pro Arg Ala Ala
1 5 10 15
Leu Phe Asp Leu Asp Gly Thr Leu Ile Asn Ser Glu Pro Arg Ser Ala
20 25 30
Ala Val Trp Ala Arg Leu Leu Arg Ala Arg Gly Ile Thr Pro Asp Glu
35 40 45
Ala Leu Leu Arg Arg Phe Met Gly Arg Arg Gly Gly Asp Val Val Ala
50 55 60
Glu Leu Pro His Leu Phe Pro Gly Glu Ser Leu Glu Gln Ile Phe Asp
65 70 75 80
Glu Leu Trp Arg His Gly Gln Asp Pro Asp Leu Pro Gln Val Ala Pro
85 90 95
Leu Pro Glu Ser Val Ala Phe Leu His His Leu His Ser Gln Gly Val
100 105 110
Pro Phe Ala Leu Val Thr Ser Ala Gly Arg Glu Trp Ala Glu Ser Ala
115 120 125
Leu Glu Trp Leu Gly Val Arg Gly Met Phe Arg Gly Leu Val Thr Ala
130 135 140
Gly Asp Val Thr Val Gly Lys Pro His Pro Gln Gly Tyr Leu Gly Gly
145 150 155 160
Ala Glu Ile Leu Gly His Glu Pro Glu His Ile Val Val Phe Glu Asp
165 170 175
Thr Pro Ala Gly Ile Met Ala Gly Arg Gly Ala Gly Met Arg Val Val
180 185 190
Gly Ile Thr Thr Thr His Pro Pro Glu Ala Leu Thr Gln Ala Asp Leu
195 200 205
Val Val Glu His Leu Thr Gln Val Ala Trp Pro Arg Leu Ser Leu Arg
210 215 220
Asp Pro Glu Pro Pro Arg Ser Ser Phe Ala Gly Arg Gly
225 230 235
<210> 182
<211> 236
<212> PRT
<213> 速生热球菌(Thermococcus celer)
<400> 182
Met Gly Arg Val Lys Ala Ile Ser Leu Asp Ile Asp Gly Thr Ile Thr
1 5 10 15
Tyr Pro Asp Arg Arg Leu His Glu Glu Ala Met Arg Ala Ile Arg Leu
20 25 30
Ala Glu Ser Leu Gly Val Pro Val Met Leu Val Thr Gly Asn Ser Val
35 40 45
Pro Phe Ala Glu Ala Met Ala Ile Met Ile Gly Thr Ser Gly Pro Val
50 55 60
Val Ala Glu Asp Gly Gly Ala Leu Ser Ile Lys Asp Gly Arg Leu Arg
65 70 75 80
Lys Arg Val Tyr Leu Thr Thr Met Asp Glu Glu Trp Ile Leu Trp Ser
85 90 95
Glu Val Lys Lys Arg Tyr Pro Gly Ala Ile Leu Ser Phe Ser Met Pro
100 105 110
Glu Arg Lys Ala Gly Leu Val Ile Leu Arg Thr Val Pro Val Glu Ala
115 120 125
Val Arg Glu Leu Ile Lys Glu Leu Gly Leu Asn Leu Ile Ala Val Asp
130 135 140
Ser Gly Phe Ala Ile His Val Lys Lys Pro Trp Ile Asn Lys Gly Thr
145 150 155 160
Gly Ile Glu Lys Ala Cys Glu Val Leu Gly Ile Lys Pro Arg Glu Val
165 170 175
Ala His Val Gly Asp Gly Glu Asn Asp Leu Asp Ala Phe Arg Val Val
180 185 190
Gly Tyr Arg Val Ala Val Gly Gln Ala Pro Glu Ser Leu Lys Gly Glu
195 200 205
Ala Asp Tyr Val Thr Glu Lys Thr Tyr Gly Ala Gly Gly Ala Glu Gly
210 215 220
Ile Leu His Val Leu Arg Glu Phe Gly Tyr Met Glu
225 230 235
<210> 183
<211> 236
<212> PRT
<213> 速生热球菌(Thermococcus celer)
<400> 183
Met Gly Arg Val Lys Ala Ile Ser Leu Asp Ile Asp Gly Thr Ile Thr
1 5 10 15
Tyr Pro Asp Arg Arg Leu His Glu Glu Ala Met Arg Ala Ile Arg Leu
20 25 30
Ala Glu Ser Leu Gly Val Pro Val Met Leu Val Thr Gly Asn Ser Val
35 40 45
Pro Phe Ala Glu Ala Met Ala Ile Met Ile Gly Thr Ser Gly Pro Val
50 55 60
Val Ala Glu Asp Gly Gly Ala Leu Ser Ile Lys Asp Gly Arg Leu Arg
65 70 75 80
Lys Arg Val Tyr Leu Thr Thr Met Asp Glu Glu Trp Ile Leu Trp Ser
85 90 95
Glu Val Lys Lys Arg Tyr Pro Gly Ala Ile Leu Ser Phe Ser Met Pro
100 105 110
Glu Arg Lys Ala Gly Leu Val Ile Leu Arg Thr Val Pro Val Glu Ala
115 120 125
Val Arg Glu Leu Ile Lys Glu Leu Gly Leu Asn Leu Ile Ala Val Asp
130 135 140
Ser Gly Phe Ala Ile His Val Lys Lys Pro Trp Ile Asn Lys Gly Thr
145 150 155 160
Gly Ile Glu Lys Ala Cys Glu Val Leu Gly Ile Lys Pro Arg Glu Val
165 170 175
Ala His Val Gly Asp Gly Glu Asn Asp Leu Asp Ala Phe Arg Val Val
180 185 190
Gly Tyr Arg Val Ala Val Gly Gln Ala Pro Glu Ser Leu Lys Gly Glu
195 200 205
Ala Asp Tyr Val Thr Glu Lys Thr Tyr Gly Ala Gly Gly Ala Glu Gly
210 215 220
Ile Leu His Val Leu Arg Glu Phe Gly Tyr Met Glu
225 230 235
<210> 184
<211> 251
<212> PRT
<213> Thermococcus litoralis
<400> 184
Met Tyr Glu Trp Asn Glu Ile Ala Leu Asn Leu Ala Lys Asp Ile Glu
1 5 10 15
Arg Glu Val Met Pro Leu Phe Gly Thr Lys Lys Ala Gly Glu Phe Ile
20 25 30
Gly Phe Ser Pro Ser Gly Asp Lys Thr Lys Leu Val Asp Lys Val Ala
35 40 45
Glu Asp Val Val Leu Glu Tyr Leu Arg Pro Leu Gly Val Asn Val Val
50 55 60
Ser Glu Glu Ile Gly Asn Ile Glu Ala Gly Ser Glu Tyr Thr Ile Val
65 70 75 80
Val Asp Pro Ile Asp Gly Ser Phe Asn Phe Ile Gln Gly Ile Pro Ile
85 90 95
Phe Gly Phe Ser Phe Ala Val Phe Lys Asn Glu Lys Pro Val Tyr Ala
100 105 110
Met Ile Tyr Glu Phe Ile Thr Lys Asn Val Tyr Glu Gly Ile Pro Gly
115 120 125
Glu Gly Ala Tyr Leu Asn Gly Glu Arg Ile Arg Val Arg His Leu Asn
130 135 140
Glu Lys Ser Ile Ser Ile Ser Phe Tyr Thr Arg Gly Arg Gly Ala Arg
145 150 155 160
Leu Val Glu Lys Val Lys Arg Thr Arg Val Leu Gly Ala Ile Ala Val
165 170 175
Glu Leu Ala Tyr Leu Ala Arg Gly Ser Leu Asp Gly Val Ile Asp Ile
180 185 190
Arg Asn Tyr Val Arg Pro Thr Asp Ile Ala Ala Gly Tyr Ile Ile Ala
195 200 205
Lys Glu Ala Gly Ala Ile Ile Thr Asp Asp Ser Gly Glu Glu Ile Lys
210 215 220
Phe Arg Leu Asp Ala Arg Glu Lys Leu Asn Ile Ile Ala Val Asn Asp
225 230 235 240
Lys Arg Leu Leu Lys Leu Ile Leu Glu Val Ile
245 250
<210> 185
<211> 237
<212> PRT
<213> 深嗜热球菌(Thermococcus profundus)
<400> 185
Met Lys Val Arg Ala Ile Ser Leu Asp Ile Asp Gly Thr Ile Thr Tyr
1 5 10 15
Arg Asp Arg Arg Leu Ser Ile Glu Ala Leu Lys Ala Ile Arg Leu Ala
20 25 30
Glu Ser Leu Gly Val Pro Val Met Leu Val Thr Gly Asn Ser Val Pro
35 40 45
Phe Ala Glu Ala Ala Ala Ile Phe Ile Gly Thr Ser Gly Pro Val Ile
50 55 60
Ala Glu Asp Gly Gly Ala Leu Ser Leu Lys Gly Lys Gly Thr Met Arg
65 70 75 80
Lys Arg Val Phe Leu Thr Asp Met Asp Glu Glu Trp Ile Leu Trp Ser
85 90 95
Glu Leu Lys Lys Arg Tyr Pro Lys Ala Glu Leu Ser Phe Ser Thr Met
100 105 110
Glu Arg Lys Ala Gly Leu Val Ile Arg Arg Thr Ile Pro Val Glu Ala
115 120 125
Val Arg Glu Ile Ile Arg Glu Leu Gly Leu Asn Leu Val Ala Val Asp
130 135 140
Ser Gly Phe Ala Ile His Val Lys Lys Pro Trp Ile Asn Lys Gly Thr
145 150 155 160
Gly Ile Glu Lys Ala Cys Glu Tyr Leu Gly Ile Ser Pro Lys Glu Val
165 170 175
Ala His Val Gly Asp Gly Glu Asn Asp Leu Asp Ala Phe Arg Val Val
180 185 190
Gly Tyr Arg Val Ala Ile Ala Gln Ala Pro Glu Ser Val Lys Glu Lys
195 200 205
Ala Asp Tyr Val Thr Gln Lys Pro Tyr Gly Asp Gly Gly Ala Glu Ala
210 215 220
Val Met His Ile Leu Arg Lys Phe Gly Tyr Leu Glu Glu
225 230 235
<210> 186
<211> 215
<212> PRT
<213> 密涅瓦热发状菌(Thermocrinis minerva)
<400> 186
Met Phe Lys Leu Val Ile Phe Asp Leu Asp Gly Thr Leu Ile Asp Ser
1 5 10 15
Cys Gln Asp Ile Thr Leu Cys Leu Asn Met Thr Leu Arg Asp Phe Gly
20 25 30
Lys Pro Gln Val Glu Ser Gln Thr Val Arg Arg Tyr Ile Gly Ser Gly
35 40 45
Ala Arg Ala Leu Leu Glu Lys Phe Phe Pro Pro Glu Asp Val Asp Lys
50 55 60
Ala Leu Glu Val Phe Arg Gly Tyr Tyr Arg Gln His Pro Val Val Tyr
65 70 75 80
Thr Arg Pro Tyr Glu Gly Ile Pro Glu Ala Leu Glu Phe Leu Arg Ser
85 90 95
Lys Gly Val Ile Met Thr Val Val Thr Asn Lys Met Glu Glu Ile Ser
100 105 110
Tyr Lys Ile Leu Asp Thr Leu Lys Leu Leu Asp Tyr Phe Ser Leu Val
115 120 125
Val Gly Gly Asp Thr Tyr His Glu Lys Lys Pro Ser Ala Leu Pro Ile
130 135 140
Ile Lys Thr Leu Glu Lys Phe Ser Val Lys Pro Lys Glu Ser Leu Ile
145 150 155 160
Val Gly Asp Thr Glu Ala Asp Ile Leu Ser Gly Lys Arg Ala Ser Val
165 170 175
Tyr Thr Ala Leu Ala Leu Trp Gly Tyr Thr Lys Asp Met Gln Glu Glu
180 185 190
Pro Asp Phe Ile Leu Asn Thr Pro Thr Glu Ile Ile Glu Leu Trp Asn
195 200 205
Ser Arg Ser Thr Ser Arg Val
210 215
<210> 187
<211> 404
<212> PRT
<213> Thermocrinis ruber
<400> 187
Met Glu Gly Gly Ser Phe Leu Asn Leu Ile Glu Ser Leu Asp Lys Phe
1 5 10 15
Ala Val Gly Ile Phe Arg Gln Phe Leu Ile Arg Glu Asp Thr Asp Lys
20 25 30
Ile Leu Ile Leu Thr Pro Asn Glu Glu Tyr Lys Arg Trp Ala Ile Ala
35 40 45
Tyr Leu Ser Thr Arg Leu Lys Gly Phe Gly Lys Lys Val Val Val Glu
50 55 60
Cys Glu Gly Glu Arg Lys Glu Gln Lys Glu Ile Glu Ser Pro Lys Asp
65 70 75 80
Asn Leu Leu Asp Lys Tyr Thr Phe Glu Asn Phe Val Val Gly Pro Ser
85 90 95
Asn Glu Leu Ala Tyr Lys Val Cys Leu Glu Val Thr Lys Asn Pro Gly
100 105 110
Lys Leu Phe Asn Pro Leu Phe Ile Tyr Gly Arg Ser Gly Leu Gly Lys
115 120 125
Thr His Leu Leu His Ala Ile Gly Asn Gln Leu Lys Ala Arg Tyr Arg
130 135 140
Val Leu Tyr Ile Pro Leu Met Asp Phe Ser Asp Ser Met Val Lys Ala
145 150 155 160
Phe Lys Glu Asn Arg Val Glu Glu Phe Arg Glu Arg Phe Phe Asn Leu
165 170 175
Asp Val Leu Leu Leu Asp Asp Val Gln Phe Leu Val Gly Lys Glu Arg
180 185 190
Thr Gln Ile Glu Leu Phe Arg Ile Tyr Glu Lys Leu Gln Ala Glu Glu
195 200 205
Lys Gln Ile Val Leu Val Ser Asp Arg His Pro Arg Asp Leu Lys Asp
210 215 220
Val Ser Glu Arg Leu Val Ser Arg Phe Glu Ser Gly Leu Ile Ile Glu
225 230 235 240
Ile Gly Leu Asp Glu Glu Thr Lys Arg Arg Ile Ile Lys Gln Lys Leu
245 250 255
Ile Leu Tyr Gly Leu Pro Leu Asp Gln Glu Thr Ile Asp Tyr Val Phe
260 265 270
Glu Asn Thr Gly Tyr Asn Val Arg Glu Ile Glu Gly Phe Val Lys Thr
275 280 285
Leu Lys Val Ser Gly Ile Lys Arg Leu Pro Lys Ser Glu Glu Leu Asp
290 295 300
Lys Glu Lys Lys Val Gln Leu Ile Ile Asn Thr Val Ala Lys Gly Phe
305 310 315 320
Lys Leu Asn Pro Glu Leu Leu Lys Lys Asp Thr Lys Glu Arg Arg Met
325 330 335
Ile Asn Ala Arg His Ile Ala Met Phe Leu Cys Lys Thr Ile Leu Asn
340 345 350
Leu Pro Tyr Ser Gln Ile Gly Glu Phe Phe Gly Lys Lys Asp His Thr
355 360 365
Ala Val Met Tyr Ala Val Lys Lys Val Glu Gln Arg Cys Arg Glu Asp
370 375 380
Arg Lys Phe Met Tyr Met Val Ser Phe Phe Glu Lys Ser Ile Arg Lys
385 390 395 400
Ser Leu Gly Leu
<210> 188
<211> 222
<212> PRT
<213> Thermoflexus hugenholtzii
<400> 188
Met Ser Arg Gly Pro Ala Phe Leu Phe Asp Leu Asp Gly Thr Leu Val
1 5 10 15
Asp Ser Val Tyr Gln His Val Leu Ala Trp Arg Glu Ala Leu Glu Ala
20 25 30
Val Gly Ile Ser Leu Ser Val Trp Arg Ile His Arg Arg Ile Gly Met
35 40 45
Ser Gly Gly Leu Leu Leu Arg Ala Leu Leu Arg Glu Thr Gly Arg Glu
50 55 60
Val Thr Ser Glu Glu Val Gln Arg Ile Gln Gln Gln His Ala Glu Ala
65 70 75 80
Phe Ala Arg Leu Leu Pro Gln Val Arg Pro Leu Pro Gly Ala Arg Glu
85 90 95
Leu Leu Glu Ala Leu Thr Arg Ala Gly Ile Pro Trp Ala Ile Ala Thr
100 105 110
Ser Gly Arg Arg Asp Val Ala Arg Pro Leu Leu Glu Leu Leu Gly Val
115 120 125
Asp Glu Gly Ile Pro Val Ile Thr Arg Glu Asp Val Pro Tyr Ala Lys
130 135 140
Pro Asp Pro Asp Leu Phe Leu Ala Ala Ala Ala Arg Leu Gly Ile Pro
145 150 155 160
Ile Ala Glu Cys Ile Val Val Gly Asp Ser Val Trp Asp Leu Leu Ala
165 170 175
Ala Arg Arg Ala Gly Ala Leu Ser Val Gly Leu Leu Ser Gly Gly Tyr
180 185 190
Gly Arg Glu Glu Leu Glu Arg Ala Gly Ala Tyr Arg Val Tyr Glu Asp
195 200 205
Pro Ala Asp Leu Leu Arg His Leu Asp Glu Val Gly Val Arg
210 215 220
<210> 189
<211> 255
<212> PRT
<213> 莱廷格热袍菌(Thermotoga lettingae)
<400> 189
Met Asp Arg Met Asp Phe Ser Ile Lys Ile Ala Arg Lys Val Gly Leu
1 5 10 15
Tyr Leu Met Glu His Trp Gly Asn Ala Glu Asn Val Arg Gln Lys Ser
20 25 30
Ser Phe Gln Asp Leu Val Ser Asp Cys Asp Lys Gln Ala Gln Lys Met
35 40 45
Ile Val Gln Lys Ile Lys Asp His Phe Pro Asp Asp Ala Ile Leu Ala
50 55 60
Glu Glu Gly Leu Phe Glu Lys Gly Asp Arg Met Trp Ile Ile Asp Pro
65 70 75 80
Ile Asp Gly Thr Met Asn Tyr Val His Gly Leu Pro Ser Phe Ala Ile
85 90 95
Gly Ile Ala Tyr Val Glu Lys Glu Gln Val Ile Leu Gly Val Ala His
100 105 110
Asp Pro Val Leu Asn Glu Thr Tyr Tyr Ala Ile Lys Gly Gln Gly Ala
115 120 125
Tyr Lys Asn Gly Glu Arg Ile Asn Val Ser Glu Asn Ser Leu Leu Lys
130 135 140
Asp Ser Ile Gly Asn Thr Gly Phe Tyr Thr Asp Phe Thr Gly Ile Phe
145 150 155 160
Ile Ser Ala Ile Glu Lys Lys Val Arg Arg Val Arg Met Thr Gly Ser
165 170 175
Ala Ile Leu Ala Gly Ala Tyr Val Ala Cys Gly Arg Phe Asp Phe Phe
180 185 190
Ile Ala Lys Arg Ala Asn Ser Trp Asp Val Ala Pro Leu Phe Val Leu
195 200 205
Val Pro Glu Ala Gly Gly Ile Val Thr Asp Leu Ser Gly Asn Gln Ala
210 215 220
His Leu Asn Thr Gly Asn Phe Leu Phe Ser Asn Gly Leu Leu His Asp
225 230 235 240
Gln Val Leu Glu Val Ile Arg Glu Val Asn Lys Lys Val Arg Lys
245 250 255
<210> 190
<211> 201
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 190
Met Arg Leu Tyr Leu Ile Arg His Gly Glu Thr Ile Trp Asn Glu Lys
1 5 10 15
Gly Leu Trp Gln Gly Ile Ala Asp Val Pro Leu Asn Glu Lys Gly Lys
20 25 30
Asp Gln Ala Lys Lys Leu Ala Glu Arg Leu Lys Arg Val Asp Ala Ile
35 40 45
Tyr Ser Ser Pro Leu Lys Arg Cys Leu Glu Thr Ala Arg Glu Ile Ala
50 55 60
Glu Arg Phe Lys Lys Glu Val Ile Val Glu Glu Asp Leu Arg Glu Cys
65 70 75 80
Glu Ile Ser Leu Trp Asn Gly Leu Thr Val Glu Glu Ala Met Arg Glu
85 90 95
Tyr Pro Glu Glu Phe Lys Arg Trp Ser Thr Asp Pro Asn Phe Gly Thr
100 105 110
Lys Gly Leu Glu Ser Met Lys Ser Val Gln Asp Arg Met Val Lys Val
115 120 125
Met Met Lys Ile Val Ser Gln Glu Arg Leu Asn Gly Ser Glu Asp Val
130 135 140
Val Ile Val Ser His Ser Leu Ser Leu Arg Ser Phe Ile Cys Trp Val
145 150 155 160
Leu Gly Leu Pro Leu Arg Leu His Arg Asn Phe Lys Leu Asp Asn Ala
165 170 175
Ser Leu Ser Ile Val Asp Val Glu Ser Arg Pro Arg Leu Val Leu Leu
180 185 190
Asn Asp Thr Cys His Leu Glu Asp Thr
195 200
<210> 191
<211> 268
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 191
Met Tyr Arg Val Phe Val Phe Asp Leu Asp Gly Thr Leu Leu Asn Asp
1 5 10 15
Ser Leu Glu Ile Ser Glu Lys Asp Arg Lys Val Ile Glu Arg Leu Ser
20 25 30
Arg Asn Cys His Val Val Phe Ala Ser Gly Arg Met Leu Val Ser Thr
35 40 45
Leu Asn Val Glu Lys Arg Tyr Phe Lys Arg Thr Phe Pro Thr Ile Ala
50 55 60
Tyr Asn Gly Ala Met Val Tyr Leu Pro Glu Glu Gly Val Val Leu Asn
65 70 75 80
Glu Lys Ile Pro Pro Glu Val Ala Lys Asp Ile Ile Glu Tyr Ile Lys
85 90 95
Pro Leu Asn Val His Trp Gln Ala Tyr Ile Asp Asp Val Leu Tyr Ser
100 105 110
Glu Lys Asp Asn Glu Glu Ile Lys Ser Tyr Ala Arg His Ser Asn Val
115 120 125
Asp Tyr Arg Val Glu Pro Asn Leu Ser Glu Leu Val Ser Lys Met Gly
130 135 140
Thr Thr Lys Leu Leu Leu Ile Asp Thr Pro Glu Arg Leu Asp Glu Leu
145 150 155 160
Lys Glu Ile Leu Ser Glu Arg Phe Lys Asp Val Val Lys Val Phe Lys
165 170 175
Ser Phe Pro Thr Tyr Leu Glu Ile Val Pro Lys Asn Val Asp Lys Gly
180 185 190
Lys Ala Leu Lys Phe Leu Arg Gly Arg Met Asn Trp Lys Lys Glu Glu
195 200 205
Ile Val Val Phe Gly Asp Asn Glu Asn Asp Leu Phe Met Phe Glu Glu
210 215 220
Ala Gly Leu Arg Val Ala Met Gly Asn Ala Ile Asp Lys Val Lys Glu
225 230 235 240
Ala Ala Asp Val Val Thr Leu Thr Asn Asn Asp Ser Gly Val Ser Tyr
245 250 255
Val Leu Glu Arg Ile Ser Thr Asp Cys Leu Asp Glu
260 265
<210> 192
<211> 201
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 192
Met Ile Arg Asn Ile Val Phe Asp Leu Gly Gly Val Leu Ile Asp Trp
1 5 10 15
Arg Pro Cys Glu Tyr Leu Val Glu Ser Phe Pro Glu Asp Val Ala Lys
20 25 30
Val Leu Glu Arg Glu Ile Phe Lys His Glu Asp Trp Lys Lys Met Asp
35 40 45
Arg Gly Thr Leu Pro Glu Asn Asp Leu Trp Glu Lys Lys Lys Lys Glu
50 55 60
Leu Ser Glu Tyr Arg Glu Tyr Val Glu Lys Leu Glu Arg Glu Val Pro
65 70 75 80
Lys Leu Leu Lys Pro Ile Glu Glu Asn Val Lys Leu Leu Ser Ile Leu
85 90 95
Lys Glu Lys Asn Phe Lys Leu Tyr Val Leu Ser Asn Tyr Gly Lys Ile
100 105 110
Tyr Phe Glu Met Val Arg Arg Arg Tyr Arg Phe Phe Asp Phe Phe Asp
115 120 125
Gly Met Val Ile Ser Ser His Val Gly Phe Ile Lys Pro Glu Lys Glu
130 135 140
Ile Tyr Leu Glu Leu Ile Arg Lys Tyr Lys Ile Thr Pro Lys Glu Ser
145 150 155 160
Leu Phe Ile Asp Asp Met Glu Glu Asn Val Lys Ala Ala Glu Glu Leu
165 170 175
Gly Phe Asn Thr Ile His Leu Pro Glu Pro Ser Arg Leu Lys Glu Leu
180 185 190
Leu Phe Glu Thr Leu Lys Ile Gly Arg
195 200
<210> 193
<211> 564
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 193
Met Leu Leu Arg Ile Ile Pro Ser Asp Arg Ile Ser Ser Thr Glu Val
1 5 10 15
Lys Thr Leu Glu Arg Val Tyr Val Val Gly His Arg Asn Pro Asp Thr
20 25 30
Asp Ser Val Cys Ser Ala Ile Gly Tyr Ala His Tyr Lys Ala Glu Val
35 40 45
Glu Lys Lys Lys Gln Phe Ile Pro Ala Arg Cys Gly Glu Leu Thr Asn
50 55 60
Glu Ala Leu Phe Val Leu Asp Tyr Phe Lys Val Arg Pro Pro Ile Phe
65 70 75 80
Leu Glu Thr Leu Glu Pro Thr Val Glu Asp Leu Glu Leu Lys Asp Pro
85 90 95
Ile Phe Val Ser Pro Asp Thr Pro Val Tyr Asp Val Ala Met Leu Met
100 105 110
Glu Gly Lys Gly Ile Lys Asn Val Pro Val Val Ser Arg Gly Lys Met
115 120 125
Ile Gly Val Val Thr Glu Ser Asn Leu Ala Arg Val Tyr Val Arg Arg
130 135 140
Leu Lys Ile Glu Pro Leu Val Ile His Pro Val Pro Leu Glu Gln Leu
145 150 155 160
Val Arg Val Leu Lys Ala Glu Val Val Cys Asp His Leu Lys Glu Lys
165 170 175
Thr Ile Ser Gly Lys Val His Ile Ala Val Asp Ala Leu His Val Leu
180 185 190
Leu Gly Lys Ile Glu Ile Gly Asp Val Val Ile Val Gly Asp Asn Glu
195 200 205
Pro Ser Gln Ile Ala Leu Leu Glu Lys Gly Ala Lys Leu Met Ile Ile
210 215 220
Val Asn Asn Ala Pro Val Ser Ser Arg Val Leu Glu Ile Ala Lys Glu
225 230 235 240
Lys Gly Ala Ala Val Leu Arg Val Lys Phe Asp Ala Phe Gly Ala Ala
245 250 255
Lys Leu Ile Asn Leu Ala Leu Pro Val Thr Leu Val Met Ser Lys Lys
260 265 270
Phe Pro Thr Val Thr Lys Arg Asp Thr Leu Glu Asp Val Lys Asn Ile
275 280 285
Val Phe Thr Ser Lys Leu Arg Ala Ala Phe Val Glu Asp Glu Lys Gly
290 295 300
Lys Leu Leu Gly Val Ile Thr Arg Thr Asp Leu Met Lys Asp Val Arg
305 310 315 320
Lys Lys Val Ile Leu Val Asp His Asn Glu Ile Thr Gln Ala Pro Glu
325 330 335
Gly Val Glu Lys Ala Glu Ile Leu Glu Ile Ile Asp His His Arg Leu
340 345 350
Gly Gly Leu Ser Thr Leu Asn Pro Val Phe Phe Tyr Asn Glu Pro Val
355 360 365
Gly Ser Thr Ser Thr Ile Val Thr Glu Phe Phe Leu Arg Asp Gly Val
370 375 380
Lys Met Glu Arg Glu Ile Ala Gly Val Leu Leu Ala Gly Ile Val Ser
385 390 395 400
Asp Thr Leu Phe Phe Lys Leu Ser Thr Thr Thr Glu Lys Asp Arg Ala
405 410 415
Met Ala Gly Phe Leu Ala Arg Ile Thr Lys Leu Asp Leu Glu Lys Phe
420 425 430
Ala Lys Lys Val Leu Lys Glu Gly Met Lys Ile Pro Glu Asn Val Asp
435 440 445
Pro Leu Asp Leu Leu Lys Arg Asp Val Lys Val Tyr Glu Met Gly Glu
450 455 460
Glu Ser Phe Ala Val Ser Gln Ile Met Thr Ser Asp Phe Ser Val Leu
465 470 475 480
Leu Lys Glu Lys Glu Lys Phe Ala Thr Ala Leu Lys Ser Leu Lys Gly
485 490 495
Glu Leu Gly Val Thr His Cys Phe Val Leu Phe Thr Asn Pro Ile Glu
500 505 510
Glu Ala Ser Leu Val Met Val Glu Gly Asp Gln Arg Ile Leu Glu Lys
515 520 525
Ala Phe Asp Gly Ala Glu Lys Lys Asp Gly Leu Phe Leu Leu Lys Gly
530 535 540
Val Met Ser Arg Lys Lys Asp Phe Val Pro Arg Ile Gly Asp Ile Leu
545 550 555 560
Arg Arg Glu Arg
<210> 194
<211> 136
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 194
Met Asp Thr Phe Leu Tyr His Ile Leu Ala Ile Asp Val Lys Gly Tyr
1 5 10 15
Val Asp Pro Ser Leu Pro Val Glu Glu Ile Val Lys Arg Leu Lys Glu
20 25 30
Gln Asn Ala Leu Val Ile Ala Ala His Pro Asp Arg Lys Lys Gln Asp
35 40 45
Glu Glu His Leu Ser Trp Phe Leu Trp Val Asn Met Glu Arg Phe Lys
50 55 60
Asp Met Phe Asp Ala Trp Glu Val Ala Asn Arg Asp Asp Leu Phe Asn
65 70 75 80
Ser Ile Gly Val Lys Lys Tyr Arg Tyr Val Ala Asn Ser Asp Phe His
85 90 95
Glu Val Trp His Val Tyr Ser Trp Lys Thr Leu Ile Arg Ser Glu Arg
100 105 110
Asn Val Glu Ala Ile Lys Glu Ala Ile Arg Lys Asn Thr Asp Val Ala
115 120 125
Ile Tyr Leu Met Arg Glu Lys Thr
130 135
<210> 195
<211> 443
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 195
Met Asn Glu Arg Asp Ile Cys Arg Arg Ile Ser Glu Ile Leu Gly Ile
1 5 10 15
Asp Tyr Asp Cys Thr Arg Gly Ile Asp Glu Leu Leu Lys Ile Leu Glu
20 25 30
Tyr Glu Ile Asn Glu Tyr Lys Lys Ser Leu Glu Glu Gln Thr Ile Phe
35 40 45
Met Glu Ala Gln Leu Glu Glu Leu Ser Arg Ser Tyr Glu Glu Ile Ser
50 55 60
Thr Leu Leu Glu Ile Ser Glu Ile Phe Gly Gly Phe Glu Phe Pro Met
65 70 75 80
Asn Leu Arg Glu Lys Leu Glu Lys Val Ile Pro Leu Leu Lys Lys Val
85 90 95
Val Lys Phe Lys Asp Tyr Ile Val Arg Val Glu Asp Leu Ser Ile Gly
100 105 110
Asn Met Ser His Glu Glu Val Glu Arg Glu Ile Glu Glu Arg Glu Lys
115 120 125
Thr Val Leu Ile Glu Pro Gly Glu Ser Lys Lys Phe Ser Asn Leu Leu
130 135 140
Phe Val Pro Ile Ile Gly Asn Arg Tyr Tyr Gly Tyr Met Cys Phe Ser
145 150 155 160
Gly Lys Glu Glu Gly Ile Val Phe Thr Ala Ser Asp Arg Arg Ile Thr
165 170 175
Glu Val Thr Ser Arg Tyr Ile Ala Asn Ala Leu Asp Arg Ile Asp Phe
180 185 190
Leu Gln Lys Glu Ile Glu Arg Gln Arg Leu Glu Glu Gln Met Glu Ile
195 200 205
Ala Arg Arg Ile Gln Ser Glu Leu Leu Pro Arg Glu Leu Pro Arg Thr
210 215 220
Gly Phe Ile Asp Ile Thr Ala Cys Ser Arg Pro Ala Val Gln Val Gly
225 230 235 240
Gly Asp Tyr Tyr Asp Val Phe Ala Ser Asp Gly Lys Val Leu Ala Val
245 250 255
Met Gly Asp Val Ala Gly Lys Ser Val Pro Ala Ala Leu Leu Met Ser
260 265 270
Ala Val Arg Ser Tyr Leu Lys Val Leu Ser Thr Ser His Ser Asp Leu
275 280 285
Glu Asp Leu Val Asn His Leu Asn Ser Ile Leu Cys Glu Asp Leu Ser
290 295 300
Asn Asp Arg Phe Val Thr Met Val Phe Leu Glu Ile Phe Gln Asn Gly
305 310 315 320
Thr Leu Asn Leu Val Asn Ala Gly His Asn Pro Val Tyr Phe Leu His
325 330 335
Asp Gly Glu Ile Val Lys Leu Glu Ala Ser Ala Ile Pro Ile Gly Ile
340 345 350
Ala Glu Trp Asn Tyr Arg Arg His Thr Ile Gln Leu Lys Pro Asn Thr
355 360 365
Phe Ile Val Val Tyr Thr Asp Gly Ile Thr Glu Ala Arg Asn Thr Leu
370 375 380
Gly Glu Glu Tyr Gly Tyr Glu Arg Leu Glu Glu Thr Leu Arg Glu Tyr
385 390 395 400
Asn Gly Asp Ser Ala Glu Asp Leu Leu Ser Lys Leu Lys Lys Ser Val
405 410 415
Glu Glu Phe Ser Lys Asp Val Pro Gln His Asp Asp Met Thr Ile Met
420 425 430
Val Leu Lys Tyr Lys Gly Gln Gln Glu Val Glu
435 440
<210> 196
<211> 266
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 196
Met Ile Ala Leu Leu Asp Met Gly Ser Asn Ser Phe Ile Leu Leu Ile
1 5 10 15
Val Ser Glu Glu Gly Glu Val Ile Leu Glu Glu Val His Glu Val Gly
20 25 30
Ile Ala Ser Gly Asn Leu Gln Arg Ala Lys Glu Val Phe Arg Glu Cys
35 40 45
Val Arg Lys Ser Glu Glu Leu Gly Ala Asp Leu His Ile Phe Gly Thr
50 55 60
Ala Phe Phe Arg Lys Asn Pro Asp Val Phe Tyr Glu Ile Thr Gly Gly
65 70 75 80
Arg Gly Lys Ile Leu Ser Glu Glu Glu Glu Ala Arg Tyr Ser Tyr Ile
85 90 95
Ser Val Val Arg Asp Phe Gly Lys Glu Asp Ile Leu Val Ala Asp Leu
100 105 110
Gly Gly Gly Ser Leu Glu Leu Ala Trp Lys Asp Gly Tyr Thr Ser Leu
115 120 125
Glu Leu Gly Thr His Ile Leu Asn Arg Ile Phe Ser Leu Thr Leu Pro
130 135 140
Phe Arg Lys Ser Val Asp Asp Val Val Glu Tyr Val Met Asp Lys Leu
145 150 155 160
Pro Asp Leu Ala Lys Ser Asp Leu Phe Gly Val Gly Gly Ser Phe Val
165 170 175
Ala Leu Ala Ala Leu Met Lys Gly Lys Trp Asp Leu Lys Ser Ile His
180 185 190
Gly Ser Thr Leu Glu Ile Glu Arg Val Gln Lys Ile Val Asp Gln Ile
195 200 205
Arg Lys Met Ser Phe Glu Asp Ile Arg Lys Leu Lys Ile Leu Pro Glu
210 215 220
Gly Arg Glu Lys Thr Ile Leu Ala Gly Gly Ile Val Thr Ile Ala Leu
225 230 235 240
Leu Lys Lys Tyr Ser Pro Lys Met Thr Val Ser Thr Lys Gly Tyr Arg
245 250 255
Tyr Gly Ile Ala Trp Glu Ile Met Val Gln
260 265
<210> 197
<211> 208
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 197
Met Glu Leu Ile Lys Leu Lys Lys Gly Gly Val Met Val Gly Thr Phe
1 5 10 15
Arg Ile Tyr Val Ala Thr Thr Asn Pro His Lys Val Glu Glu Ile Lys
20 25 30
Glu Ile Ala Pro Glu Trp Ala Glu Ile Leu Pro Ser Pro Glu Lys Ile
35 40 45
Glu Val Ile Glu Asp Gly Glu Thr Phe Leu Glu Asn Ser Val Lys Lys
50 55 60
Ala Ile Val Tyr Gly Lys Lys Leu Lys Ser Pro Val Ile Ala Asp Asp
65 70 75 80
Ser Gly Leu Val Ile Tyr Ser Leu Gly Gly Phe Pro Gly Val Met Ser
85 90 95
Ala Arg Phe Met Glu Glu Tyr Thr Tyr Glu Glu Lys Met Lys Thr Ile
100 105 110
Leu Lys Met Leu Glu Gly Lys Asp Arg Lys Ala Ala Phe Val Cys Ser
115 120 125
Ala Thr Phe Phe Asp Phe Gln Arg Asn Leu Leu Val Ser Val Glu Asp
130 135 140
Arg Val Glu Gly Tyr Ile Ala Glu Glu Ile Arg Gly Thr Gly Gly Phe
145 150 155 160
Gly Tyr Asp Pro Phe Phe Val Pro Glu Gly Tyr Asp Arg Thr Phe Gly
165 170 175
Glu Met Pro Glu Leu Lys Lys Lys Leu Ser His Arg Ser Arg Ala Phe
180 185 190
Arg Lys Leu Phe Ser Ile Leu Ser Lys Ile Ser Glu Ser Glu Ser Leu
195 200 205
<210> 198
<211> 436
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 198
Met Ile Gln Val Arg Arg Leu Ile Val Leu Val Leu Ala Leu Leu Leu
1 5 10 15
Val Ala Phe Ser Phe Ser Gln Val Lys Asn Ile Ile Tyr Phe Ile Gly
20 25 30
Asp Gly Met Gly Leu Ser Gln Ala Tyr Leu Thr Ser Leu Ile Glu Gly
35 40 45
Arg Pro Leu Ser Phe Met Lys Thr Pro Tyr Thr Gly Leu Val Lys Thr
50 55 60
His Ser Val Asp Ser Trp Val Thr Asp Ser Ala Ala Ala Gly Thr Ala
65 70 75 80
Leu Ala Ser Gly Phe Lys Thr Lys Asn Gly Met Ile Asn Val Leu Pro
85 90 95
Asp Gly Thr Arg Val Pro Thr Ile Phe Glu Ile Ala Lys Ala Tyr Gly
100 105 110
Ala Lys Thr Gly Ile Val Val Thr Cys Arg Val Thr His Ala Thr Pro
115 120 125
Ala Ala Phe Tyr Ala His Val Lys Ser Arg Ser Glu Glu Asn Glu Ile
130 135 140
Ala Arg Gln Leu Val Glu Ser Glu Thr Val Asp Leu Val Met Gly Gly
145 150 155 160
Gly Trp Ala Asn Phe Leu Pro Glu Ser Leu Gly Gly Lys Arg Ser Asp
165 170 175
Gly Leu Asn Leu Ile Glu Met Ala Lys Lys Lys Gly Tyr Val Tyr Val
180 185 190
Thr Lys Lys Glu Asp Leu Met Lys Leu Pro Glu Asn Thr Glu Lys Val
195 200 205
Leu Ala Leu Phe Ala Pro Ser His Leu Asp Pro Ala Ser Ser Arg Glu
210 215 220
Glu Gln Pro Met Leu Tyr Glu Met Val Lys Lys Ala Leu Glu Ile Leu
225 230 235 240
Ser Asn Asp Glu Glu Pro Phe Phe Leu Met Val Glu Gly Ser Gln Ile
245 250 255
Asp Trp Glu Ala His Asp Asn Asp Ile Tyr Gly Val Trp Lys Glu Val
260 265 270
Val Glu Phe Asp Arg Ala Val Gln Val Ala Leu Asp Phe Ala Leu Lys
275 280 285
Arg Gly Asp Thr Leu Val Ile Val Thr Ala Asp His Glu Thr Gly Gly
290 295 300
Leu Ala Leu Ser Ser Gly Asp Tyr Arg Val Asp Val Asp Arg Ile Arg
305 310 315 320
Lys Phe Ser Lys Thr Thr Asp Trp Ile Leu Ala Asn Tyr Ser Leu Lys
325 330 335
Asp Arg Glu Ser Phe Lys Lys Ala Ile Glu Lys Tyr Phe Gly Leu Thr
340 345 350
Leu Ser Asp Ser Asp Leu Asp Arg Ile Thr Ser Ser Asn Asn Ser Lys
355 360 365
Val Glu Leu Gly Arg Val Leu Ser Glu Lys Val Asn Val Gly Trp Thr
370 375 380
Thr Thr Ser His Ser Gly Val Pro Val Pro Ile Tyr Ala Phe Gly Pro
385 390 395 400
Gly Ala Glu Asn Phe Thr Gly Phe Leu Asp Asn Thr Asp Ile Pro Arg
405 410 415
Ile Met Met Lys Leu Ala Gly Tyr Ser Leu Gln Tyr Pro Leu Val Lys
420 425 430
Glu Pro Val Lys
435
<210> 199
<211> 174
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 199
Met Lys Ile Leu Ile Thr Gly Arg Pro Gly Val Gly Lys Thr Thr Leu
1 5 10 15
Ile Lys Lys Ile Ser Ser Leu Leu Gln Asn Ala Gly Gly Phe Tyr Thr
20 25 30
Glu Glu Met Arg Glu Lys Gly Lys Arg Val Gly Phe Lys Ile Val Thr
35 40 45
Leu Asp Gly Arg Glu Gly Ile Leu Ala Lys Val Gly Phe Pro Ser Gln
50 55 60
His Arg Val Gly Lys Tyr Gly Val Asn Leu Lys Asp Leu Glu Glu Leu
65 70 75 80
Gly Val Asp Ser Val Glu Arg Ala Leu Glu Glu Lys Ser Val Val Ile
85 90 95
Ile Asp Glu Ile Gly Lys Met Glu Leu Leu Ser Glu Arg Phe Lys Arg
100 105 110
Val Val Glu Lys Ala Phe Asn Ser Glu Lys Asp Leu Ile Ala Thr Ile
115 120 125
Lys Lys Ser Ser Asp Pro Phe Val Glu Lys Ile Lys Asn Lys Lys Asp
130 135 140
Val Ile Ile Phe Glu Leu Asn Glu Arg Asn Arg Asp Leu Leu Leu Lys
145 150 155 160
Arg Ile Leu Asp Met Leu Lys Ser Asn Arg Gly Val Gly Glu
165 170
<210> 200
<211> 508
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 200
Met Arg Arg Tyr Leu Val Leu Phe Leu Val Leu Leu Leu Ser Val Phe
1 5 10 15
Leu Leu Gly Val Arg Leu Thr Ile Leu His Val Asn Asp Thr His Gly
20 25 30
His Ala Trp Thr Phe Asp Glu Tyr Gln Asn Pro Gly Ile Gly Gly Leu
35 40 45
Ala Ala Ile Ala Thr Ile Val Glu Glu Val Lys Arg Glu Val Glu Glu
50 55 60
Gln Gly Gly Tyr Val Ile Phe Leu His Ala Gly Asp Leu Asn Thr Gly
65 70 75 80
Val Pro Glu Ser Asp Leu Gln Asp Ala Ile Pro Asp Ile Val Gly Tyr
85 90 95
Asn Met Ile Gly Leu Ser Ala Met Ala Val Gly Asn His Glu Phe Asp
100 105 110
Asn Pro Arg Asp Val Leu Leu Lys Gln Met Lys Phe Ala Asp Phe Pro
115 120 125
Phe Leu Ser Ala Asn Ile Leu Lys Glu Asn Gly Glu Pro Phe Phe Thr
130 135 140
Pro Tyr Ile Leu Lys Asp Phe Gly Lys Leu Lys Val Ala Ile Ile Gly
145 150 155 160
Phe Thr Thr Glu Glu Thr Thr Ile Leu Glu Pro Leu Tyr Leu Glu Asp
165 170 175
Leu Lys Phe Glu Asn Ala Leu Lys Val Ala Gln Lys Ile Ala Pro Glu
180 185 190
Leu Lys Asn Gln Ala Asp Val Val Ile Ala Leu Ala His Leu Asp Trp
195 200 205
Gly Glu Pro Lys Lys Glu Asn Ile Thr Thr Thr His Gln Leu Ala Lys
210 215 220
Val Asp Gly Ile Asp Val Val Val Ala Gly His Ser His Val Leu Gly
225 230 235 240
Ser Glu Val Val Asp Glu Lys Ile Ile Val Ser Ala Gly Glu Tyr Gly
245 250 255
Lys Tyr Val Gly Arg Leu Asp Leu Asp Ile Glu Asp Gly Lys Ile Val
260 265 270
Ala Trp His Trp Gln Ala Ile Pro Val Asn Leu Lys Ala Tyr Arg Asp
275 280 285
Gly Lys Tyr Glu Tyr Val Gly Lys Pro Tyr Leu Glu Asn Arg Tyr Val
290 295 300
Ala Lys Ala Leu Glu Tyr Phe Arg Lys Val Gly Asn Glu Lys Leu Asp
305 310 315 320
Thr Val Ile Gly Glu Thr Lys Ile Phe Leu Asp Gly Glu Arg Glu His
325 330 335
Val Arg Ser Arg Asn Thr Asn Leu Thr Asn Leu Ile Thr Asp Ala Met
340 345 350
Arg Trp Lys Val Lys Ala Asp Ile Ala Leu Thr Asn Gly Glu Gly Ile
355 360 365
Arg Ala Ser Ile Lys Pro Gly Lys Ile Thr Val Arg Asp Ile Leu Thr
370 375 380
Val Leu Pro Phe Gly Asn Thr Leu Tyr Val Leu Glu Leu Thr Gly Glu
385 390 395 400
Gln Ile Met Lys Val Leu Glu Tyr Ser Ala Thr Ile Pro Glu Gly Lys
405 410 415
Gly Ala Phe Leu Gln Val Ser Gly Leu Thr Trp Lys Ser Lys Asp Gly
420 425 430
Lys Val Val Glu Val Leu Val Asn Gly Glu Pro Leu Asp Pro Lys Lys
435 440 445
Thr Tyr Arg Val Val Thr Asn Asn Tyr Met Ala Gly Gly Gly Asp Gly
450 455 460
Tyr Thr Met Leu Lys Glu Trp Gly Gly Tyr Asp Thr Gly Phe Leu Met
465 470 475 480
Ser Asp Ala Val Ile Glu Tyr Ile Gln Asn Val Leu Asn Gly Val Ile
485 490 495
Glu Glu Tyr Asp Asn Ser Gln Arg Tyr Val Arg Glu
500 505
<210> 201
<211> 154
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 201
Met Asp Ala Lys Ile Val Asn Ala Leu Ile Gly Ser Val Tyr Glu Thr
1 5 10 15
Ile Lys Ser Val Leu Arg Val Glu Pro Lys Leu Gly Lys Pro Ser Ala
20 25 30
Val Ser His Ile Glu Ile Pro His Ser Val Val Thr Val Ile Gly Val
35 40 45
Thr Gly Ser Ile Glu Gly Ser Leu Ile Tyr Ser Phe Ser Ser Glu Thr
50 55 60
Ala Leu Lys Val Val Ser Ala Met Met Gly Met Glu Tyr Gly Gln Leu
65 70 75 80
Asp Glu Leu Ala Met Ser Ala Ile Gly Glu Leu Gly Asn Met Thr Ala
85 90 95
Gly Lys Leu Ala Met Lys Leu Glu Thr Leu Gly Lys His Val Asp Ile
100 105 110
Thr Pro Pro Thr Val Val Ser Gly Lys Asp Leu Lys Ile Lys Ser Phe
115 120 125
Gly Thr Val Leu Lys Leu Pro Ile Ser Val Phe Ser Asn Glu Asp Phe
130 135 140
Asp Leu His Leu Ser Val Lys Ser Gly Gly
145 150
<210> 202
<211> 333
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 202
Met Lys Glu Ile Val Asp Ala Ile Ser Gln Tyr Asn Arg Ile Leu Val
1 5 10 15
Val Gly His Ile Met Pro Asp Gly Asp Cys Val Ser Ser Val Leu Ser
20 25 30
Leu Thr Leu Gly Leu Glu Arg Ile Gly Lys Glu Val Arg Ala Ala Ile
35 40 45
Asp Tyr Arg Val Pro Tyr Val Phe Glu Asn Phe Pro His Ile Glu Arg
50 55 60
Ile Glu Gln Asn Val Asp Phe Glu Pro Glu Leu Val Ile Val Val Asp
65 70 75 80
Ala Ser Ser Pro Asp Arg Ile Gly Arg Phe Gln Asp Leu Leu Lys Glu
85 90 95
Val Pro Ser Ala Val Ile Asp His His Ser Thr Asn Val Leu Phe Gly
100 105 110
Lys Trp Asn Trp Val Asp Pro Ser Phe Ala Ala Thr Ala Gln Met Val
115 120 125
Phe Arg Leu Asn Arg Lys Leu Gly Val Glu Tyr Asp Pro Val Leu Ala
130 135 140
Thr Leu Asn Tyr Leu Gly Ile Ala Thr Asp Thr Gly Phe Phe Arg His
145 150 155 160
Ser Asn Thr Asp Ala Arg Val Phe Glu Asp Ala Tyr Glu Leu Val Lys
165 170 175
Ile Gly Ala Asp Ala His Phe Val Ala Lys Glu Ile Leu Glu Asn Lys
180 185 190
Arg Phe Glu Gln Phe Lys Leu Phe Ala Glu Val Leu Glu Arg Leu Gln
195 200 205
Leu Leu Glu Asn Gly Arg Ile Ala Tyr Ser Tyr Ile Asp Tyr Asp Thr
210 215 220
Tyr Leu Arg His Asn Cys Thr Asp Glu Asp Ser Ala Gly Phe Val Gly
225 230 235 240
Glu Leu Arg Ser Ile Arg Gly Val Glu Val Ala Val Leu Phe Met Glu
245 250 255
Phe Pro Lys Gly Lys Ile His Val Ser Met Arg Ser Lys Asp Trp Phe
260 265 270
Asn Val Asn Glu Val Ala Phe Glu Leu Gly Gly Gly Gly His Pro Arg
275 280 285
Ala Ala Gly Val Thr Phe Glu Gly Glu Lys Leu Glu Asn Val Ile Ser
290 295 300
Arg Val Ile Asp His Leu Leu Gly Arg Phe Lys Glu Gly Val Ser Arg
305 310 315 320
Glu Gly Glu Lys Thr Ser Gln Arg Ser Val Leu Gly Arg
325 330
<210> 203
<211> 295
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 203
Met Ile Leu Arg Arg Arg Gly Ile Val Leu Ser Phe His Ser Asn Met
1 5 10 15
Val Thr Val Glu Asp Glu Glu Thr Gly Glu Arg Leu Leu Cys Lys Leu
20 25 30
Arg Gly Lys Phe Arg Leu Gln Asn Leu Lys Ile Tyr Val Gly Asp Arg
35 40 45
Val Glu Tyr Thr Pro Asp Gly Thr Gly Ser Gly Val Ile Glu Asn Val
50 55 60
Leu His Arg Lys Asn Leu Leu Val Lys Pro His Val Ala Asn Val Asp
65 70 75 80
Gln Ala Ile Leu Val Val Thr Val Lys Met Pro Glu Thr Ser Thr Tyr
85 90 95
Ile Ile Asp Lys Phe Leu Val Leu Thr Glu Lys Asn Glu Leu Glu Thr
100 105 110
Val Leu Val Ile Asn Lys Met Asp Ile Tyr Asp Glu Glu Asp Leu Glu
115 120 125
Lys Val Lys Glu Leu Glu Arg Ile Tyr Ser Lys Leu Tyr Pro Ile Val
130 135 140
Lys Thr Ser Ala Lys Thr Gly Met Gly Ile Glu Glu Leu Lys Lys Tyr
145 150 155 160
Leu Lys Gly Lys Ile Ser Thr Met Ala Gly Leu Ser Gly Val Gly Lys
165 170 175
Ser Ser Leu Leu Asn Ala Ile Asn Pro Gly Leu Lys Leu Arg Val Ser
180 185 190
Glu Val Ser Gln Lys Leu Gln Arg Gly Arg His Thr Thr Thr Ser Ala
195 200 205
Gln Leu Leu Arg Phe Asp Phe Gly Gly Tyr Val Val Asp Thr Pro Gly
210 215 220
Phe Ala Asn Leu Glu Ile Gly Asp Ile Glu Pro Glu Glu Leu Lys Tyr
225 230 235 240
Tyr Phe Lys Glu Phe Asn Glu Lys Gln Cys Phe Phe Ser Asp Cys Asn
245 250 255
His Ile Asp Glu Pro Glu Cys Gly Val Lys Glu Ala Val Glu Asn Gly
260 265 270
Glu Ile Ala Glu Ser Arg Tyr Glu Asn Tyr Val Lys Met Phe His Glu
275 280 285
Leu Leu Gly Arg Gly Lys Ser
290 295
<210> 204
<211> 528
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 204
Met Lys Lys Ile Thr Gly Arg Phe Leu Lys Thr Ile Ile Lys Lys Ala
1 5 10 15
Thr Glu Asn Leu Leu Lys His Lys Asp Glu Ile Asn Ala Leu Asn Val
20 25 30
Phe Pro Val Pro Asp Gly Asp Thr Gly Ser Asn Met Cys Ser Thr Met
35 40 45
Leu Glu Ala Cys Lys Tyr Ile Asp Asn Leu Lys Ser Asp Asp Leu Ser
50 55 60
Glu Val Trp Lys Ala Ile Lys Glu Gly Ala Leu Met Gly Ala Arg Gly
65 70 75 80
Asn Ser Gly Val Ile Leu Ser Gln Ile Leu Arg Gly Leu Ala Asp Ala
85 90 95
Ser Pro Glu Asn Tyr Ile Thr Pro Gly Asp Phe Ile Lys Met Ile Ser
100 105 110
Asn Ala Arg Lys Val Ala Tyr Ser Ala Val Met Arg Pro Val Glu Gly
115 120 125
Thr Met Leu Thr Val Val Arg Glu Leu Asp Glu Lys Leu Lys Gly Arg
130 135 140
Ser Phe Glu Thr Phe Glu Glu Leu Phe Asp Gln Ile Val Glu Met Ile
145 150 155 160
Lys Asp Thr Val Asn Arg Thr Pro Ser Met Leu Ser Lys Leu Arg Glu
165 170 175
Ala Gly Val Val Asp Ala Gly Ala Lys Gly Leu Tyr Tyr Leu Phe Glu
180 185 190
Gly Met Arg Asp Ala Ile Lys Gly Asp Ile Glu Val Asn Leu Glu Gln
195 200 205
Val Glu Gln Ala Ser Val Glu Asp Leu Lys Arg Met Ala Leu Glu Glu
210 215 220
Ile Thr Asn Gln Tyr Cys Thr Glu Val Ala Val Arg Arg Lys Arg Val
225 230 235 240
Phe Glu Lys Ser Glu Leu Glu Ser Phe Leu Asn Glu Ile Gly Asp Ser
245 250 255
Val Val Leu Val Glu Gln Asp Asp Ile Phe Arg Leu His Val His Thr
260 265 270
Asn His Pro Gly Gln Val Leu Glu Lys Val Leu Asp Phe Gly Glu Ile
275 280 285
Ile Lys Val Lys Val Asp Asn Met Lys Leu Gln His Glu His Ile Ile
290 295 300
Ser Ala Gln Val Glu Lys Glu Ile Gly Val Val Ala Val Ser Pro Gly
305 310 315 320
Lys Gly Ile Ser Glu Ile Leu Lys Ser Leu Gly Val Asp Glu Ile Val
325 330 335
Pro Gly Gly Gln Thr Met Asn Pro Ser Phe Ala Asp Leu Lys Ala Ala
340 345 350
Val Asp Lys Thr His Ala Lys Val Val Phe Leu Phe Pro Asn Asn Ala
355 360 365
Asn Val Leu Leu Thr Ala Lys Gln Val Ala Glu Ser Val Asp Asp Lys
370 375 380
Arg Val Ile Val Val Gln Thr Ser His Val Gln Glu Cys Val Ala Ala
385 390 395 400
Met Val Glu Tyr Asp Pro Asp Glu Asp Pro Glu Glu Leu Lys Arg Arg
405 410 415
Phe Glu Glu Ala Ile Asn Gln Cys Val Pro Ile Ser Ile Thr Arg Ala
420 425 430
Val Arg Asp Ser Arg Tyr Gly Lys Arg Arg Ile Arg Lys Gly Glu Tyr
435 440 445
Leu Val Phe Val Arg Lys Glu Leu Leu Ala His Gly Phe Asn Leu Val
450 455 460
Lys Ala Leu Lys Asp Val Leu Glu Lys Glu Asn Ala Arg Glu Lys Glu
465 470 475 480
Ile Leu Thr Val Phe Leu Gly Asp Asn Tyr Arg Lys Ala Glu Leu Glu
485 490 495
Lys Ile Gln Asn Leu Ile Gly Glu Glu Phe Pro Asn Leu Asp Leu Glu
500 505 510
Ile His Glu Gly Gly Gln Pro His Tyr Pro Phe Leu Met Leu Leu Gln
515 520 525
<210> 205
<211> 263
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 205
Met Glu Gly Gly Ile Glu Leu Asp Arg Leu Asp Phe Ser Ile Lys Leu
1 5 10 15
Leu Arg Arg Val Gly His Phe Leu Met Leu His Trp Gly Lys Val Asp
20 25 30
Ser Val Glu Lys Lys Thr Gly Phe Lys Asp Ile Val Thr Glu Ile Asp
35 40 45
Lys Lys Ala Gln Glu Met Ile Val Glu Glu Ile Arg Lys Val Phe Pro
50 55 60
Asp Glu Asn Ile Ile Ala Glu Glu Gly Ile Ser Glu Asn Gly Lys Lys
65 70 75 80
Leu Trp Ile Ile Asp Pro Ile Asp Gly Thr Ile Asn Phe Val His Gly
85 90 95
Leu Pro Asn Phe Ser Ile Ser Ile Ala Tyr Val Glu Asn Gly Glu Val
100 105 110
Lys Met Gly Val Val His Ala Pro Ala Leu Asn Glu Thr Leu Tyr Ala
115 120 125
Glu Glu Asn Gly Gly Ala Phe Leu Asn Gly Glu Arg Ile Arg Val Ser
130 135 140
Gly Asn Thr Ser Leu Glu Glu Cys Val Gly Ser Thr Gly Ser Tyr Val
145 150 155 160
Asp Phe Thr Gly Lys Phe Ile Glu Lys Met Glu Lys Lys Thr Arg Arg
165 170 175
Val Arg Ile Leu Gly Ser Ala Ala Leu Asn Ala Cys Tyr Val Gly Ala
180 185 190
Gly Arg Val Asp Phe Phe Val Thr Trp Arg Ile Asn Pro Trp Asp Ile
195 200 205
Ala Ala Gly Leu Ile Val Val Lys Glu Ala Gly Gly Thr Val Thr Asp
210 215 220
Phe Ala Gly Lys Glu Ala Asn Val Phe Ser Lys Asn Phe Val Phe Ser
225 230 235 240
Asn Gly Leu Val His Glu Glu Val Leu Glu Val Val Asn Glu Val Leu
245 250 255
Lys Glu Ile Gly Glu Gly Lys
260
<210> 206
<211> 90
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 206
Met Lys Ala Leu Lys Ile Arg Val Glu Gly Ile Val Gln Gly Val Gly
1 5 10 15
Phe Arg Tyr Phe Thr Arg Arg Ile Ala Arg Thr Leu Gly Ile Lys Gly
20 25 30
Tyr Val Met Asn Met Asp Asp Gly Ser Val Phe Ile His Ala Glu Gly
35 40 45
Glu Glu Glu Lys Leu Arg Arg Phe Leu Asn Glu Val Ser Lys Gly Pro
50 55 60
Pro Ala Ala Val Val Thr Asn Ile Ser Val Glu Glu Thr Ser Pro Glu
65 70 75 80
Gly Tyr Glu Asp Phe Ala Ile Arg Tyr Tyr
85 90
<210> 207
<211> 401
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 207
Met Leu Ser Phe Phe Cys Glu Thr Pro Phe Gln Ile Leu Pro Tyr Gly
1 5 10 15
Lys Glu Glu Leu Asp Ser Met Gln Leu Glu Arg Leu Asp Glu Gly Leu
20 25 30
Phe Tyr Pro His Tyr Asn Gly Tyr Ser Ile Val Asn Phe Ser Asn Thr
35 40 45
Ile Leu Ser Ile Phe Gly Glu Lys Thr Leu His Arg Pro Phe Pro Leu
50 55 60
Pro Glu Arg Phe Leu Glu Asn Val Arg Lys Val Val Val Leu Val Val
65 70 75 80
Asp Ala Leu Ser Tyr Asp Val Phe Ser Asn Thr Ile Gln Asp Asp Asp
85 90 95
Val Gln Val Phe Pro Cys Ser Ser Val Phe Pro Thr Thr Thr Ala Ala
100 105 110
Ala Leu Pro Ser Leu Tyr Ser Gly Leu Thr Pro Leu Glu His Gly Phe
115 120 125
Leu Gly Tyr Ile Leu Tyr Leu Arg Glu Val Gly Ser Pro Val Asn Met
130 135 140
Ile Glu Met Ala Pro Pro Gly Phe Pro Arg Glu Ser Val Leu Arg Ile
145 150 155 160
His Glu Phe Arg Phe Thr Thr Ile Phe Gln Arg Leu Lys Val Arg Ser
165 170 175
Phe Phe Leu Val Pro Arg Tyr Leu Leu Gly Thr Gly Phe Ser Arg Leu
180 185 190
Met Ser Gln Gly Ala Glu Gln Ile Gly Phe Ser Ser Phe Gly Asp Met
195 200 205
Ile Glu Lys Thr Leu Glu Ile Leu Ala Asn Gln Glu Thr Val Leu Val
210 215 220
Phe Ala Tyr Trp Pro Ser Leu Asp Ser Ile Ala His Lys Ser Gly Val
225 230 235 240
Gly Arg Ala Tyr Phe Arg Glu Leu Lys Trp Leu Tyr Arg Ile Leu Lys
245 250 255
Glu Glu Leu Ile Arg Arg Leu Lys Ser Asp Thr Leu Phe Phe Met Val
260 265 270
Ser Asp His Gly Gln Ile Ser Thr Pro Pro Glu Arg Glu Val Trp Trp
275 280 285
Asp Ser Phe Ser Glu Val Met Arg Phe Leu Asp Arg Pro Pro Ala Gly
290 295 300
Glu Gln Arg Met Met Tyr Leu Tyr Thr Lys Arg Lys Lys Ala Leu Val
305 310 315 320
Glu Tyr Leu Val Glu Lys Tyr Ala Asp Phe Ala Ile Phe Ile Asp Ala
325 330 335
Arg Arg Ala Thr Arg Leu Phe Gly Ile Gly Lys Ser His Pro Glu Phe
340 345 350
Phe His Arg Ile Gly Asp Leu Ile Leu Ile Thr Lys Glu Asn Phe Ser
355 360 365
Phe Asn Tyr Arg Tyr Thr Gly Lys Glu Glu Ser Leu Ser Gly Arg His
370 375 380
Gly Ser Leu Thr His Gln Glu Leu Val Val Pro Leu Val Val Tyr Arg
385 390 395 400
Arg
<210> 208
<211> 229
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 208
Met Lys Ala Asp Leu His Val His Thr Cys Leu Ser Pro Cys Ala Asp
1 5 10 15
Leu Leu Met Ile Pro Pro Val Ile Glu Lys Ala Glu Val Asp Ile Leu
20 25 30
Gly Ile Val Asp His Asn Ser Ala Arg Asn Ala Leu Ala Phe Ser Lys
35 40 45
Met Lys Lys Leu Val Ile Pro Gly Val Glu Ile Gln Thr Val Glu Asp
50 55 60
Val His Leu Leu Gly Phe Phe Val Asn Phe Glu Asp Ala Leu Lys Leu
65 70 75 80
Thr Glu Val Leu Tyr Glu His Leu Pro Arg Leu Lys His Asp His Glu
85 90 95
Lys Met Gly Tyr Gln Leu Leu Val Asn Glu Asp Gly Asp Tyr Ile Gly
100 105 110
Tyr Glu Asn Met Pro Leu Gly Phe Pro Ala Asn Leu Thr Ile Ser Gln
115 120 125
Ala Val Asp Leu Val Leu Ser Phe Arg Gly Val Pro Ala Tyr Ala His
130 135 140
Val Glu Arg Arg Phe Gly Val Leu Tyr Gln Leu Gly Phe Phe Pro Asn
145 150 155 160
Leu Asp Val Pro Val Ala Glu Val Val Ser Glu Glu Gly Arg Ser Lys
165 170 175
Ala Glu Gly Arg Phe Arg Val Ile Val Ser Ser Asp Ala His Phe Pro
180 185 190
Asp Glu Val Gly Arg Arg Tyr Ile Glu Leu Lys Gly Lys Pro Asp Ser
195 200 205
Pro Lys Lys Val Leu Asp Gln Ile Leu Ala Gly Glu Tyr Thr Leu Gly
210 215 220
Gly Val Leu Asp Trp
225
<210> 209
<211> 122
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 209
Met Leu Asp Val Trp Lys Ile Phe Arg Ser Thr Pro Phe Thr Thr Ala
1 5 10 15
Val Val Ser Phe Leu Val Ala Gln Phe Ile Lys Phe Leu Ile Lys Arg
20 25 30
Asp Val Lys Met Leu Lys Ser Tyr Gly Gly Met Pro Ser Gly His Val
35 40 45
Ala Thr Val Ser Gly Leu Ala Trp Ser Leu Ala Arg Ser Thr Gly Phe
50 55 60
Asp Ser Pro Tyr Thr Ser Ile Ala Ser Ile Phe Leu Val Ile Ile Phe
65 70 75 80
Met Asp Ala Ile Val Leu Arg Pro Ala Val Lys Lys Asp Leu Gly His
85 90 95
Ser Phe Leu Glu Ala Leu Ala Gly Phe Gly Leu Gly Met Leu Ile Ala
100 105 110
His Leu Phe Pro Ala Arg Leu His Leu Trp
115 120
<210> 210
<211> 181
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 210
Met Thr Val Lys Phe Tyr Glu Gln Lys Ile Glu Ser Lys Arg Val Phe
1 5 10 15
Glu Gly Lys Met Ile Ser Val Arg Val Asp Arg Val Arg Leu Pro Asn
20 25 30
Gly Glu Glu Ser Thr Arg Glu Val Val Asp His Pro Gly Ala Val Val
35 40 45
Ile Val Pro Val Leu Gly Glu Glu Ile Ile Phe Val Glu Gln Tyr Arg
50 55 60
Tyr Pro Ile Glu Gln Met Leu Leu Glu Leu Pro Ala Gly Lys Met Asp
65 70 75 80
Pro Gly Glu Ser Pro Glu Glu Cys Ala Lys Arg Glu Leu Glu Glu Glu
85 90 95
Thr Gly Tyr Arg Ala Lys Arg Phe Ser Tyr Leu Gly Lys Ile Phe Thr
100 105 110
Thr Pro Gly Phe Thr Thr Glu Val Ile His Ile Phe Ala Ala Glu Glu
115 120 125
Leu Glu Lys Thr Thr Gln Asn Thr Asp Pro Asp Glu Phe Ile Glu Val
130 135 140
Lys Lys Ile Pro Val Glu Lys Val Leu Ser Leu Leu Arg Asn Ala Glu
145 150 155 160
Ile Glu Asp Ser Lys Thr Ile Cys Ala Leu Thr Arg Tyr Phe Leu Ser
165 170 175
Lys Gly Val Ile Gly
180
<210> 211
<211> 224
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 211
Met Lys Lys Ala Val Leu Phe Asp Leu Asp Gly Thr Ile Leu Asp Phe
1 5 10 15
Glu Lys Ser Glu Glu Thr Ala Leu Lys Lys Thr Phe Leu Arg His Gly
20 25 30
Ile Pro Leu Thr Glu Glu Gln Val Leu Leu Tyr Lys Ser Ile Asn Arg
35 40 45
Lys Trp Trp Lys Met Leu Ala Glu Lys Lys Val Ser Lys Glu Glu Val
50 55 60
Val Val Ala Arg Phe Glu Glu Phe Leu Gly Glu Ile Gly Ser Leu Leu
65 70 75 80
Asp Pro Glu Glu Val Ala Lys Glu Tyr Leu Glu Phe Leu Ser Glu Glu
85 90 95
Ala Tyr Phe Leu Pro Gly Ala Glu Asp Phe Leu Lys Glu Leu Lys Arg
100 105 110
Asn Gly Phe Arg Met Ala Ala Val Thr Asn Gly Val Arg Phe Val Gln
115 120 125
Glu Arg Arg Ser Lys Lys Leu Gly Leu Glu Arg Phe Phe Glu Phe Val
130 135 140
Leu Thr Ser Glu Glu Val Gly Val Glu Lys Pro Asp Pro Arg Ile Phe
145 150 155 160
Trp Ile Ala Leu Glu Arg Met Gly Leu Lys Lys Glu Asp Ala Leu Tyr
165 170 175
Val Gly Asp Asp Pro Ala Ser Asp Leu Glu Gly Ala Arg Asn Ala Gly
180 185 190
Ile Asp Phe Val Leu Phe Ser Pro Ser Gly Asp Val Ser Arg Glu Phe
195 200 205
Pro Val Val Arg Asn Phe Glu Glu Leu Arg Glu Ile Leu Arg Ile Leu
210 215 220
<210> 212
<211> 204
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 212
Met Asn Ile Ser Glu Arg Gln Lys Asp Leu Leu Lys Glu Ile Gly Asn
1 5 10 15
Ile Gly Ala Gly Asn Ala Ala Thr Ala Ile Ser Tyr Met Val Asn Lys
20 25 30
Lys Val Glu Ile Ser Val Pro Thr Val Glu Ile Ile Pro Leu Ser Asp
35 40 45
Val Ile Phe Val Ala Lys Asp Pro Glu Glu Ile Val Val Gly Val Lys
50 55 60
Met Pro Val Thr Gly Glu Ile Glu Gly Asn Val Leu Leu Ile Met Gly
65 70 75 80
Thr Gln Val Val Lys Lys Ile Leu Glu Ile Leu Ile Gly His Ala Pro
85 90 95
Glu Asn Leu Leu Gln Leu Asp Glu Phe Ala Ala Ser Ala Leu Gln Glu
100 105 110
Val Gly Asn Ile Met Cys Gly Thr Tyr Val Ser Ala Leu Ala Asp Phe
115 120 125
Leu Gly Phe Lys Ile Asp Thr Leu Pro Pro Gln Leu Val Val Asp Met
130 135 140
Ile Ser Ala Ile Phe Ala Glu Val Ser Ile Glu Glu Leu Gly Glu Ser
145 150 155 160
Val Asp Asp Gln Val Val Phe Val Glu Thr Ser Leu Thr Val Glu Glu
165 170 175
Glu Glu Pro Leu Thr Ser Tyr Leu Met Leu Val Pro Lys Pro Gly Tyr
180 185 190
Leu Lys Lys Ile Phe Glu Arg Met Gly Val Gln Glu
195 200
<210> 213
<211> 236
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 213
Met Glu Met Leu Leu Gly Ile Val Gln Gly Leu Thr Glu Phe Leu Pro
1 5 10 15
Val Ser Ser Ser Gly His Leu Val Leu Phe Ser His Val Phe Lys Met
20 25 30
Asp Leu Asn Ala Tyr Gln Thr Ala Ile Leu His Leu Gly Thr Leu Ala
35 40 45
Ala Val Val Val Phe Ala Leu Glu Gly Ile Arg Arg Ser Leu Arg Asn
50 55 60
Trp Arg Val Ile Leu Asn Leu Val Ile Ser Thr Ile Pro Ala Gly Val
65 70 75 80
Phe Gly Val Leu Phe Glu Ser Arg Val Asp Glu Ala Phe Ser Ser Pro
85 90 95
Val Val Leu Pro Leu Phe Phe Ser Phe Thr Ala Leu Val Leu Leu Phe
100 105 110
Thr Ser Ser Phe Ser Gly Glu Lys Arg Met Glu Glu Met Thr Ile Leu
115 120 125
Asp Ala Thr Leu Val Gly Leu Ala Gln Val Leu Ala Leu Phe Pro Gly
130 135 140
Val Ser Arg Ser Gly Ile Thr Ile Ala Thr Leu Leu Phe Leu Lys Tyr
145 150 155 160
Arg Arg Glu Asp Ala Leu Gln Tyr Ser Phe Leu Met Ser Ile Pro Val
165 170 175
Val Leu Gly Ala Gly Ile Leu Gly Met Glu Lys Gly Asn Ile Ser Leu
180 185 190
Thr Ala Pro Phe Phe Ala Phe Leu Ser Gly Ile Leu Ala Leu Tyr Ile
195 200 205
Leu Ser Arg Ser Val Arg Ser Gly Lys Met Trp Gln Phe Ala Tyr Tyr
210 215 220
Cys Leu Phe Val Ala Ile Val Ser Tyr Phe Val Gly
225 230 235
<210> 214
<211> 233
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 214
Met Ile Asp Met His Leu His Ser Thr Phe Ser Tyr Asp Gly Lys Ala
1 5 10 15
Glu Val Glu Asp Ile Ile Ala Gln Val Gln Lys Leu Gly Ile Asn His
20 25 30
Phe Cys Ile Thr Asp His Tyr Glu Tyr Glu Asn Gly Lys Leu Val His
35 40 45
Asp Phe Asn Val Glu Glu Tyr Phe Leu Thr Met Asp Lys Phe Asp Leu
50 55 60
Pro Arg Gly Ala Glu Ile Ser Trp Asp Gly Val Lys Glu Ala Ile Phe
65 70 75 80
Pro Glu Gly Phe Asp Tyr Leu Leu Leu Gly Val His Arg Tyr Asp Glu
85 90 95
Asn Leu Pro Pro Asp Glu Leu Ala Arg Asp Tyr Leu Glu Arg Thr Leu
100 105 110
Tyr Val Met Glu Arg Val Glu Phe His Thr Leu Ala His Leu Asp Tyr
115 120 125
Pro Ala Arg Tyr Ala Asn Ala Asp Phe Lys Ala Asn Arg Asp Leu Ile
130 135 140
Glu Lys Ile Leu Arg Phe Leu Val Ala Arg Glu Lys Ile Leu Glu Val
145 150 155 160
Asn Thr Ala Gly Leu Phe Lys His Gly Lys Pro Asn Pro Asp Tyr Trp
165 170 175
Ile Leu Glu Met Tyr Trp Asp Leu Gly Gly Arg Phe Ile Thr Ile Gly
180 185 190
Ser Asp Ala His Glu Val Gln His Ile Gly Arg Gly Ile Glu Glu Val
195 200 205
Met Ser Lys Leu Arg Arg Phe Asn Phe Glu Tyr Val Val Val Glu Gly
210 215 220
Lys Lys Leu Phe Thr Lys Lys Leu Arg
225 230
<210> 215
<211> 227
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 215
Met Val Asn Val Val Met Val Pro Cys Ser Val Val Lys Asp Glu Thr
1 5 10 15
Val Val Val Val Asp Val Leu Arg Ala Thr Ser Thr Ile Val Thr Ala
20 25 30
Leu Ala Asn Gly Ala Lys Glu Val Ile Pro Val Lys Thr Val Glu Glu
35 40 45
Ala Leu Lys Arg Lys Glu Arg Asp Val Leu Val Cys Gly Glu Arg Asn
50 55 60
Ala Lys Lys Ile Glu Gly Phe Asp Leu Gly Asn Ser Pro Leu Glu Tyr
65 70 75 80
Lys Ser Glu Leu Val Leu Gly Lys Thr Ile Val Leu Thr Thr Thr Asn
85 90 95
Gly Thr Gln Val Ile Glu Arg Val Glu Gly Asp Ser Val Ile Ala Ala
100 105 110
Ser Phe Leu Asn Val Ser Ala Val Val Glu Tyr Leu Lys Glu Lys Glu
115 120 125
Asn Ile Thr Ile Val Cys Ala Gly Thr Ser Gly Asp Phe Ser Leu Glu
130 135 140
Asp Phe Leu Leu Ala Gly Met Ile Val Lys Arg Met Lys Arg Glu Asp
145 150 155 160
Leu Leu Asp Gly Ala Leu Val Val Met Arg Tyr Phe Glu Ser Val Gln
165 170 175
Asn Ile Arg Glu Glu Ile Lys Arg Phe Ser Ser His Ala Lys Arg Leu
180 185 190
Ile Ser Leu Gly Phe Glu Lys Asp Val Asp Phe Cys Thr Thr Glu Asp
195 200 205
Leu Phe His Glu Val Pro Val Leu Val Asn Gly Ala Phe Thr Leu Lys
210 215 220
Glu Ala Arg
225
<210> 216
<211> 209
<212> PRT
<213> 那不勒斯栖热袍菌(Thermotoga neapolitana)
<400> 216
Met Thr Tyr Ala Val Gly Asp Ile His Gly Cys Phe Phe Ala Leu Lys
1 5 10 15
Ala Leu Leu Glu Lys Leu Pro Leu Gly Lys Glu Asp Glu Leu Val Phe
20 25 30
Leu Gly Asp Tyr Val Asp Arg Gly Pro Asn Ser Lys Glu Val Val Glu
35 40 45
Phe Leu Met Glu Leu Ser Lys His His Arg Cys Ile Phe Leu Arg Gly
50 55 60
Asn His Glu Glu Met Leu Leu Asn Cys Val Lys Asn His Ser Gly Cys
65 70 75 80
Asp Leu Trp Tyr Phe Asn Gly Ala Arg Ser Thr Val Glu Ser Phe Gly
85 90 95
Gly Ile Asp Glu Ile Arg Lys Tyr Leu Asp Phe Phe Glu Ser Thr Val
100 105 110
Tyr Tyr Tyr Glu Lys Gly Asn Phe Val Phe Val His Gly Gly Val Lys
115 120 125
Pro Gly Ile Pro Leu Glu Glu Gln Asp Pro Phe Asp Leu Val Trp Ile
130 135 140
Arg Glu Glu Phe Ile Tyr Ser Glu Asn Pro Leu Pro Gly Lys Thr Val
145 150 155 160
Val Phe Gly His Thr Pro Leu Glu Lys Pro Tyr Val Ser Ser Asp Lys
165 170 175
Ile Gly Ile Asp Thr Gly Cys Val Tyr Gly Gly Lys Leu Thr Ala Phe
180 185 190
Arg Val Glu Asp Arg Thr Phe Phe Gln Val Glu Cys Ile Gly Arg Arg
195 200 205
Trp
<210> 217
<211> 264
<212> PRT
<213> 嗜石油热袍菌(Thermotoga petrophilia)
<400> 217
Met Phe Val Phe Asp Leu Asp Gly Thr Leu Leu Asn Asp Ser Leu Glu
1 5 10 15
Ile Ser Glu Lys Asp Arg Arg Ala Ile Glu Arg Leu Ser Arg Lys Cys
20 25 30
Arg Val Val Phe Ala Ser Gly Arg Met Leu Val Ser Thr Leu Asn Val
35 40 45
Glu Lys Lys Tyr Phe Gly Arg Thr Phe Pro Thr Ile Ala Tyr Asn Gly
50 55 60
Ala Met Val Tyr Ile Pro Glu Glu Gly Val Ile Leu Asn Glu Lys Ile
65 70 75 80
Pro Pro Glu Val Ala Lys Asp Ile Val Glu Tyr Val Lys Gln Phe Asn
85 90 95
Val His Trp Gln Ala Tyr Ile Asp Asp Val Leu Tyr Ser Glu Lys Asp
100 105 110
Asn Glu Glu Ile Arg Gly Tyr Ala Lys His Ser Ser Val Glu Tyr Arg
115 120 125
Val Glu Pro Lys Leu Leu Glu Leu Val Ser Lys Val Gly Thr Thr Lys
130 135 140
Ile Leu Leu Ile Asp Thr Pro Glu Lys Leu Asp Glu Leu Lys Lys Ile
145 150 155 160
Leu Leu Glu Lys Phe Asn Asn Val Val Lys Val Phe Lys Ser Phe Pro
165 170 175
Thr Tyr Leu Glu Ile Val Pro Glu Asn Val Asp Lys Gly Lys Ala Leu
180 185 190
Arg Phe Leu Arg Glu Arg Met Gly Trp Lys Lys Glu Glu Ile Val Val
195 200 205
Phe Gly Asp Asn Glu Asn Asp Leu Phe Met Phe Glu Glu Ala Gly Leu
210 215 220
Arg Val Ala Met Gly Asn Ala Ile Asp Lys Val Lys Glu Ala Ala Asp
225 230 235 240
Val Val Thr Leu Thr Asn Asn Asp Ser Gly Val Ser His Val Leu Glu
245 250 255
Leu Ile Ser Thr Asp Cys Leu Asp
260
<210> 218
<211> 208
<212> PRT
<213> Thermus amyloliquefaciens
<400> 218
Met Leu Lys Ala Leu Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp Thr
1 5 10 15
Asp Pro Leu His Leu Gln Ala Trp Arg Glu Ala Leu Ala Pro Trp Gly
20 25 30
Leu Gln Val Asp Glu Ala Phe Tyr Arg Lys Arg Ile Ser Gly Arg Leu
35 40 45
Asn Pro Asp Ile Val Gln Asp Leu Leu Gly Leu Glu Gly Glu Ala Ala
50 55 60
Gln Arg Leu Ile Glu Ala Lys Glu Ala Arg Phe Arg Glu Leu Ala Gln
65 70 75 80
Asp Leu Lys Pro Thr Pro Gly Leu His Gly Leu Leu Ala Lys Ala Glu
85 90 95
Ala Gln Gly Leu Thr Trp Gly Val Val Thr Asn Ala Pro Arg Glu Asn
100 105 110
Ala Leu His Val Leu Arg Ala Leu Gly Leu Asn Pro Pro Leu Leu Val
115 120 125
Leu Ala Glu Glu Val Gly Arg Gly Lys Pro Asp Pro Leu Pro Tyr Arg
130 135 140
Val Ala Leu Glu Arg Leu Gly Ile Glu Pro Gly Glu Ala Leu Ala Phe
145 150 155 160
Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly Ile Pro
165 170 175
Thr Tyr Ala Leu Leu Thr Gly His Gly Lys Glu Ala Leu Leu Glu Ala
180 185 190
Gly Ala Leu Glu Ala Leu Arg Asp Phe Gln Gln Ile Val Asp Leu Leu
195 200 205
<210> 219
<211> 260
<212> PRT
<213> 丝状栖热菌(Thermus filiformis)
<400> 219
Met Lys Val Ser Gln Ala His Leu Glu Ala Ala Ile Asp Ala Ala Phe
1 5 10 15
Leu Ala Gln Gly Ile His Thr Tyr Tyr Leu Glu Arg Gly Phe Thr Gln
20 25 30
Lys Thr Lys Ser Gly Pro Thr Asp Leu Val Thr Gln Ala Asp Lys Glu
35 40 45
Ser Glu Glu Ala Ile Lys Asp Leu Leu Leu Ser Arg Phe Pro Asp His
50 55 60
Gly Phe Leu Gly Glu Glu Gly Gly Gly Asp Ala Gly Gly Gly Val Arg
65 70 75 80
Trp Val Val Asp Pro Leu Asp Gly Thr Val Asn Tyr Ala His Gly Phe
85 90 95
Pro Phe Tyr Gly Val Ser Ile Ala Leu Glu Val Glu Gly Arg Val Glu
100 105 110
Leu Gly Val Val Leu Asp Thr Ala Arg Gly Glu Leu Phe Thr Ala Leu
115 120 125
Arg Gly Gln Gly Ala Tyr Leu Asn Gly Arg Pro Ile Arg Val Thr Glu
130 135 140
Arg Thr Glu Leu Leu Gly Ser Leu Leu Ala Thr Gly Phe Pro Tyr Asp
145 150 155 160
Val Ala Arg Asp Pro Glu Asn Leu Thr Tyr Phe Glu Arg Ala Leu Arg
165 170 175
Arg Gly Leu Leu Val Arg Arg Pro Gly Ala Ala Ala Leu Asp Leu Ala
180 185 190
Tyr Val Ala Ala Gly Arg Leu Asp Gly Phe Trp Glu Val Lys Leu Asn
195 200 205
Pro Trp Asp Val Ala Ala Gly Trp Leu Leu Val Glu Glu Ala Gly Gly
210 215 220
Arg Val Thr Asp Leu Glu Gly Glu Ala Tyr Arg Leu Gly His Arg Tyr
225 230 235 240
Ile Val Ala Thr Asn Gly Arg Ile His Gln Ala Leu Leu Ala Val Leu
245 250 255
Arg Gly Thr Met
260
<210> 220
<211> 208
<212> PRT
<213> 嗜热栖热菌(Thermus thermophilus)
<400> 220
Met Leu Lys Ala Val Leu Phe Asp Leu Asp Gly Thr Leu Ala Asp Thr
1 5 10 15
Asp Pro Leu His Leu Leu Ala Trp Arg Glu Ala Leu Ala Pro Tyr Gly
20 25 30
Leu Glu Val Asp Pro Ile Phe Tyr Arg Lys Arg Ile Ser Gly Arg Leu
35 40 45
Asn Pro Glu Ile Val Arg Asp Leu Leu Gly Leu Glu Gly Glu Glu Ala
50 55 60
Glu Arg Leu Ile Ala Ala Lys Glu Ala Arg Phe Arg Ala Leu Ala Gln
65 70 75 80
Gly Leu Arg Pro Thr Pro Gly Leu Ala Glu Phe Leu Glu His Ile Arg
85 90 95
Glu Lys Gly Leu Leu Trp Gly Val Val Thr Asn Ala Pro Lys Glu Asn
100 105 110
Ala Arg His Val Leu Glu Ala Leu Gly Leu Arg Pro Pro Leu Leu Val
115 120 125
Leu Ala Glu Glu Val Gly Arg Gly Lys Pro Asp Pro Leu Pro Tyr Arg
130 135 140
Val Ala Leu Arg Arg Leu Gly Val Ala Pro Glu Glu Ala Leu Ala Phe
145 150 155 160
Glu Asp Ser Pro Ser Gly Val Arg Ser Ala Val Gly Ala Gly Ile Pro
165 170 175
Thr Tyr Gly Leu Leu Thr Gly His Glu Ala Glu Ala Leu Arg Glu Ala
180 185 190
Gly Ala Arg Gly Val Phe Arg Asp Phe Arg Glu Ala Leu Gly Leu Leu
195 200 205
<210> 221
<211> 264
<212> PRT
<213> 嗜热栖热菌(Thermus thermophilus)
<400> 221
Met Ile Gly Arg Arg His Pro Tyr Tyr Pro Tyr Leu Glu Ala Ala Leu
1 5 10 15
Glu Ala Ala Ser Leu Ala Arg Gly Ile His Leu Tyr Tyr Leu Glu Lys
20 25 30
Gly Phe Thr Glu Gly Thr Lys Ser Gly Pro Thr Asp Leu Val Thr Gln
35 40 45
Ala Asp Arg Glu Ala Glu Glu Ala Val Lys Gly Leu Leu Leu Ser Arg
50 55 60
Phe Pro Glu Ala Gly Phe Leu Gly Glu Glu Gly Gly Ser Glu Gly Gly
65 70 75 80
Lys Ala Leu Arg Phe Ile Val Asp Pro Leu Asp Gly Thr Val Asn Tyr
85 90 95
Ala His Gly Phe Pro Phe Phe Ala Val Ser Ile Ala Leu Glu Ala Glu
100 105 110
Gly Ala Ile Gln Met Gly Val Val Met Asp Thr Ala Arg Gly Glu Val
115 120 125
Phe Tyr Ala Leu Arg Gly Glu Gly Ala Tyr Leu Asn Gly Arg Pro Ile
130 135 140
Arg Val Thr Gly Arg Glu Ser Leu Val Gly Ser Leu Leu Ala Thr Gly
145 150 155 160
Phe Pro Tyr Asp Val Ala Lys Asp Pro Glu Asn Leu Thr Tyr Phe Glu
165 170 175
Arg Ala Leu Gly Lys Gly Leu Leu Val Arg Arg Pro Gly Ala Ala Ala
180 185 190
Leu Asp Leu Ala Tyr Val Ala Ala Gly Arg Leu Glu Gly Phe Trp Glu
195 200 205
Val Lys Leu Asn Pro Trp Asp Val Ala Ala Gly Trp Leu Leu Val Glu
210 215 220
Glu Ala Gly Gly Arg Val Thr Asp Leu Glu Gly Asn Pro Tyr Arg Leu
225 230 235 240
Gly Ser Arg Tyr Ile Leu Ala Thr Asn Gly Arg Val His Glu Ala Leu
245 250 255
Arg Arg Thr Leu Leu Gly Leu Asp
260
<210> 222
<211> 229
<212> PRT
<213> Truepera radiovictrix
<400> 222
Met Gly His Pro Lys Ala Val Leu Leu Asp Val Asp Gly Thr Leu Val
1 5 10 15
Asp Ser Asn Asp Ala His Ala Arg Ala Tyr Val Asp Ala Leu Lys Ala
20 25 30
Tyr Gly Tyr Asp Val Pro Phe Glu Lys Val Arg Pro Leu Ile Gly Met
35 40 45
Gly Gly Asp Lys Leu Leu Pro Gln Val Ile Asp Val Glu Ser Asp Ala
50 55 60
Pro Leu Arg Glu Glu Ile Asp Ala Arg Arg Gly Glu Ile Phe Lys Ser
65 70 75 80
Arg Tyr Leu Pro Asp Ile Lys Pro Phe Pro Gly Val Arg Glu Leu Leu
85 90 95
Glu Arg Leu Arg Arg Asp Gly Leu Thr Leu Val Ala Ala Ser Ser Ala
100 105 110
Lys Glu Asp Glu Ala Asp Ala Leu Leu Glu Arg Ala Gly Val Ala Asp
115 120 125
Leu Ile Asp Ala Arg Thr Ser Ser Asp Asp Ala Glu Asn Ser Lys Pro
130 135 140
Asp Pro Asp Ile Leu Gln Ala Ala Leu Glu Arg Ala Gly Val Ser Ala
145 150 155 160
Gln Glu Ala Leu Leu Leu Gly Asp Thr Pro Tyr Asp Leu Gln Ala Ala
165 170 175
Ala Pro Leu Gly Leu Gly Val Val Ala Leu Arg Cys Gly Gly Trp Arg
180 185 190
Asp Glu Asp Leu Leu Gly Ala Leu Ala Val Tyr Asp Asp Pro Gln Ala
195 200 205
Leu Leu Ala Gly Tyr Asp Ala Ser Pro Leu Ala Pro Glu Pro Ala Ser
210 215 220
Ala Pro Gly Asp Val
225

Claims (14)

1.阿洛酮糖-6-磷酸磷酸酶用于制备阿洛酮糖的用途，其中
所述阿洛酮糖-6-磷酸磷酸酶由SEQ ID NO：6、12至14、17、18、20至23、25、26、28、29、37至53、55至57、59至61、64至66、69、70、72、73、76、77、80、81、85、88、92、95、99至105、108、113、114、116、117、122、131、133、134、136、142至147、150至152、154至156、159至163、167、169、172、173、175、184至187、189、191、192、195、198、205、210、211、214、216、217和221的任一氨基酸序列组成，并且表现出对阿洛酮糖-6-磷酸的选择性活性。
2.权利要求1所述的用途，其中
所述阿洛酮糖-6-磷酸磷酸酶由SEQ ID NO：6、12、26、29、38至43、45至53、56、57、59、60、64至66、69、70、72、76、80、81、92、95、99至103、113、114、116、117、131、134、136、142、145、146、167、169、172、184至187、189、191、192、211、217和221的任一氨基酸序列组成。
3.转化子用于制备阿洛酮糖的用途，其中所述转化子包含编码权利要求1或2所述的阿洛酮糖-6-磷酸磷酸酶的核酸。
4.组合物用于制备阿洛酮糖的用途，其中所述组合物包含
权利要求1或2所述的阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物。
5.权利要求4的用途，其中
所述组合物还包括磷酸糖。
6.一种制备阿洛酮糖的方法，其包括
通过使阿洛酮糖-6-磷酸与阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物接触，以将阿洛酮糖-6-磷酸转化为阿洛酮糖，其中所述阿洛酮糖-6-磷酸磷酸酶由SEQ ID NO：6、12至14、17、18、20至23、25、26、28、29、37至53、55至57、59至61、64至66、69、70、72、73、76、77、80、81、85、88、92、95、99至105、108、113、114、116、117、122、131、133、134、136、142至147、150至152、154至156、159至163、167、169、172、173、175、184至187、189、191、192、195、198、205、210、211、214、216、217和221的任一氨基酸序列组成，并且表现出对阿洛酮糖-6-磷酸的选择性活性。
7.权利要求6的方法，其中所述方法还包括
在将阿洛酮糖-6-磷酸转化为阿洛酮糖之前，通过使果糖-6-磷酸与果糖-6-磷酸-3-差向异构酶、表达所述酶的微生物或所述微生物的培养物接触，以将果糖-6-磷酸转化为阿洛酮糖-6-磷酸。
8.权利要求7的方法，其中所述方法还包括
在将果糖-6-磷酸转化为阿洛酮糖-6-磷酸之前，通过使葡萄糖-6-磷酸与葡萄糖-6-磷酸异构酶、表达所述酶的微生物或所述微生物的培养物接触，以将葡萄糖-6-磷酸转化为果糖-6-磷酸。
9.权利要求8的方法，其中所述方法还包括
在将葡萄糖-6-磷酸转化为果糖-6-磷酸之前，通过使葡萄糖-1-磷酸与磷酸葡萄糖变位酶、表达所述酶的微生物或所述微生物的培养物接触，以将葡萄糖-1-磷酸转化为葡萄糖-6-磷酸。
10.权利要求8的方法，其中所述方法还包括
在将葡萄糖-6-磷酸转化为果糖-6-磷酸之前，通过使葡萄糖与聚磷酸葡萄糖激酶、表达所述酶的微生物或所述微生物的培养物以及聚磷酸盐接触，以将葡萄糖转化为葡萄糖-6-磷酸。
11.权利要求9的方法，其中所述方法还包括
在将葡萄糖-1-磷酸转化为葡萄糖-6-磷酸之前，通过使淀粉、麦芽糊精、蔗糖或其组合与α-葡聚糖磷酸化酶、淀粉磷酸化酶、麦芽糊精磷酸化酶或蔗糖磷酸化酶；表达所述酶的微生物；或所述微生物的培养物以及磷酸盐接触，以将淀粉、麦芽糊精、蔗糖或其组合转化为葡萄糖-1-磷酸。
12.权利要求11的方法，其中所述将淀粉、麦芽糊精、蔗糖或其组合转化为葡萄糖-1-磷酸还包括
通过进一步包含α-淀粉酶、支链淀粉酶、异淀粉酶、α-葡聚糖转移酶、葡萄糖淀粉酶或蔗糖酶；表达所述酶的微生物；或所述微生物的培养物，以将淀粉、麦芽糊精、蔗糖或其组合转化为麦芽低聚糖或葡萄糖。
13.一种制备阿洛酮糖的方法，其包括
使淀粉、麦芽糊精、蔗糖或其组合以及磷酸盐与(a)i)阿洛酮糖-6-磷酸磷酸酶，其中所述阿洛酮糖-6-磷酸磷酸酶由SEQ ID NO：6、12至14、17、18、20至23、25、26、28、29、37至53、55至57、59至61、64至66、69、70、72、73、76、77、80、81、85、88、92、95、99至105、108、113、114、116、117、122、131、133、134、136、142至147、150至152、154至156、159至163、167、169、172、173、175、184至187、189、191、192、195、198、205、210、211、214、216、217和221的任一氨基酸序列组成，并且表现出对阿洛酮糖-6-磷酸的选择性活性；ii)果糖-6-磷酸-3-差异构酶；iii)葡萄糖-6-磷酸异构酶；iv)磷酸葡萄糖变位酶或葡萄糖激酶；以及v)α-葡聚糖磷酸化酶、淀粉磷酸化酶、麦芽糊精磷酸化酶、蔗糖磷酸化酶、α-淀粉酶、支链淀粉酶、异淀粉酶、葡萄糖淀粉酶、α-葡聚糖转移酶、聚磷酸葡萄糖激酶或蔗糖酶；或(b)表达(a)项所述酶的微生物或所述微生物的培养物接触。
14.权利要求7-13中任一项所述方法，所述接触是在5.0至9.0的pH、40℃至80℃的温度下进行，和/或进行2小时至24小时或120小时。
CN201980089137.XA 2018-12-11 2019-12-05 新阿洛酮糖-6-磷酸磷酸酶，包含其制备阿洛酮糖的组合物，和用其制备阿洛酮糖的方法 Active CN113544265B (zh)

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