WO2016088996A1 - Composition, using natural extracts, for treating and preventing periodontal diseases - Google Patents

Abstract

The present invention relates to a composition, using natural extracts, for treating and preventing periodontal diseases and, specifically, to a composition, using a natural extract, for treating and preventing periodontal diseases, characterized by containing calcium, an avocado extract, a water-soluble propolis extract, a corn extract, a cinnamon extract, a thistle extract, vitamin C, vitamin B1, vitamin B2, and vitamin B6. The composition for treating and preventing periodontal diseases is prepared using the natural extracts, and thus can be used in conjunction with dental treatment, so that the composition can be helpful in the treatment of periodontal diseases, and patients can take the composition for a long period of time even without depending on antibiotics. In addition, the composition contains all of the ingredients, which are excellent in three functions necessary for dental treatment, that is, a bone formation function, an immune enhancement function, and a periodontitis prevention and treatment function, and thus the composition has excellent effects of preventing and treating periodontal diseases.

Description

Composition for the treatment and prevention of periodontal disease using natural extracts

The present invention relates to a composition for the treatment and prevention of periodontal disease using a natural extract, and more specifically, because the composition for the treatment and prevention of periodontal disease using a natural extract can be used in combination with dental treatment in the treatment of periodontal disease. It relates to a composition for the treatment and prevention of periodontal disease using a natural extract that can be helpful and can be taken long-term without resorting to antibiotics.

In modern society, the number of people with poor eating habits and the aging of the immune system is reduced due to aging or periodontal disease due to nutritional and metabolic disorders is increasing. Nutraceuticals and therapeutic agents having the effect of preventing or treating periodontal disease so that the periodontal disease can be ingested are currently being developed.

However, synthetic drug periodontal disease treatments made of synthetic materials can cause side effects such as misuse of antibiotics due to the use of antibiotics, making it difficult to take periodontal disease treatments for a long time, and most of the commonly used treatments for periodontal disease are treatment of periodontal disease. Many problems arise as it is marketed in an unproven state. In order to solve this problem, it is necessary to develop a treatment and prevention of periodontal disease using natural extracts, that is, plant extracts.

Republic of Korea Patent Publication No. 10-0431170 is a composition for the treatment of periodontal disease containing plant extracts, cinnamon, missing, Hojang-geun, gojija, bokbunja, eoseongcho, browning, dansam extract selected from the group of 1 or 2 It provides a composition for treating periodontal disease containing more than one species as an active ingredient, excellent plant growth inhibitory activity of periodontal disease-causing bacteria, plant extracts excellent in inhibiting activity of proteolytic enzymes secreted by periodontal disease-causing bacteria The present invention discloses a composition for treating periodontal disease, which is excellent in treating periodontal disease.

In addition, the Republic of Korea Patent Publication No. 10-1358507 relates to a composition for anti-inflammatory, antibacterial and deodorant in the oral cavity containing red ginseng and natural plant-derived extracts, oral with anti-inflammatory, antibacterial and deodorant effects derived from red ginseng and natural plants It discloses a composition for anti-inflammatory, antibacterial and deodorant and a method for preparing the same.

In addition, Korean Laid-open Patent Publication No. 10-2012-0117387 relates to oral compositions for preventing and alleviating gum disease, which is beneficial to gum and dental health such as kelp, shiitake mushroom, green tea extract, tocopherol, and propolis. It discloses a composition for oral cavity having the gum and dental health and oral cleansing effect using the ingredients as a raw material.

Natural extracts, namely, the treatment and prevention of periodontal disease using plant extracts have been developed a lot, but the composition for the prevention and treatment related to chronic periodontal disease or peri-implantitis has not been developed yet, and treatment and prevention effect of periodontal disease At the same time, it helps to form bones and includes functional elements that enhance immunity, thus maximizing the effect of periodontal disease treatment, and treating and preventing periodontal disease effective treatment for chronic periodontal disease and peri-implantitis. It wasn't.

[Preceding technical literature]

[Patent Documents]

Republic of Korea Patent Publication No. 10-0431170

Republic of Korea Patent Publication No. 10-1358507

Republic of Korea Patent Application Publication No. 10-2012-0117387

The present invention has been derived to solve the problems as described above, the purpose of one embodiment of the present invention is to use the natural extracts to prepare a composition for the treatment and prevention of periodontal disease because it can be used in conjunction with dental treatment periodontal periodontal It is to provide a composition for treating and preventing periodontal disease using a natural extract that can be useful for the treatment of the disease, and can be taken for a long time without resorting to antibiotics. More specifically, calcium, avocado extract, corn extract and vitamin C and bone soluble propolis extract, corn extract, cinnamon and vitamin C, which help bone formation, and thistle extract to help boost immunity. Chronic periodontal disease and peri-implantitis because the composition for the treatment and prevention of periodontal disease is prepared to include all of vitamin B1, vitamin B2 and vitamin B6. It is an object of the present invention to provide a composition for the treatment and prevention of periodontal disease using natural extracts effective for the prevention and treatment of patients.

As one embodiment of the present invention for achieving the above object, one embodiment of the present invention is calcium, avocado extract, water soluble propolis extract, corn extract, cinnamon extract, thistle extract, vitamin C, vitamin B1, vitamin B2 And it provides a composition for the treatment and prevention of periodontal disease using a natural extract, characterized in that it comprises a vitamin B6.

Composition for treating and preventing periodontal disease using the natural extract is calcium 35 to 60% by weight, avocado extract 0.5 to 7% by weight, water soluble propolis extract 5 to 25% by weight, corn extract 2 to 15% by weight, cinnamon extract 0.5 to 7 wt%, thistle extract 2-15 wt%, vitamin C 1-10 wt%, vitamin B1 0.01-0.3 wt%, vitamin B2 0.01-0.3 wt% and vitamin B6 0.01-0.3 wt% You can do

In addition, to the composition for the treatment and prevention of periodontal disease using the natural extract may be characterized in that the addition of any one or more from the group consisting of additives consisting of silicon dioxide, magnesium stearate, crystalline cellulose and a color coating agent in the form of a tablet form. have.

In addition, 0.1 to 1 parts by weight of silicon dioxide, 0.1 to 1.5 parts by weight of magnesium stearate, 12 to 26 parts by weight of crystalline cellulose and a color coating agent based on 100 parts by weight of the composition for treating and preventing periodontal disease using the natural extract. It may be characterized in that it is added to the ratio of 1 to 5 parts by weight to prepare a formulation in tablet form.

The composition for treating and preventing periodontal disease using natural extracts according to one embodiment of the present invention is a composition for treating and preventing periodontal disease that can be ingested in parallel with dental treatment, and can be ingested at the same time as treatment with dental treatment. It can help prevent periodontal disease and treat periodontal disease.

In addition, because it is a composition for the treatment and prevention of periodontal disease, which is composed of natural extracts, not antibiotics or chemical raw materials, it can minimize the side effects that can occur in the human body due to the abuse of antibiotics, and can be taken for a long period of time periodontal disease As well as preventing and can increase the treatment effect of periodontal disease.

Composition for treating and preventing periodontal disease using a natural extract according to an embodiment of the present invention, including all three components that are excellent in bone formation function, immune function enhancement function, periodontal inflammation prevention and treatment functions required for dental care Because of this, the effect of preventing and treating periodontal disease is excellent.

Without preparing a composition for treating and preventing periodontal disease only with ingredients such as water-soluble propolis extract, corn extract, cinnamon extract and vitamin C, which help to strengthen the alveolar bone and periodontal ligament around teeth, Since the ingredient for promoting bone formation and the ingredient for enhancing immunity were added together to prepare a composition for the treatment and prevention of periodontal disease, the effect of preventing and treating periodontal disease than the existing composition for treating and preventing periodontal disease Has the advantage of being superior.

In other words, it strengthens the alveolar bone and periodontal ligament around the teeth to help prevent and treat periodontal inflammation, and to maintain components such as water-soluble propolis extract, corn extract, cinnamon extract and vitamin C and implant prosthesis It contains ingredients such as calcium, avocado extract, corn extract and vitamin C, which help prevent and treat inflammation around the implant, and helps to boost the body's overall immunity. By adding ingredients such as thistle extract, vitamin B1, vitamin B2 and vitamin B6, which are directly conducive to skin inflammation, by preparing a composition for treating and preventing periodontal disease using natural extracts to include all 10 components, bone Helps in formation, prevents and treats periodontal disease, and Since the force is enhanced there is a remarkably advantage that the prevention and treatment of existing periodontal disease treatment and prevention of periodontal disease the composition than for.

1 is a questionnaire of the questionnaire conducted for the adjuvant composition for treatment and prevention of periodontal disease according to an embodiment of the present invention.

2 and 3 is a periodontal disease that occurs when two or more weeks of taking the drug W recipe (Recipe W) prepared with the adjuvant composition for treatment and prevention of periodontal disease according to an embodiment of the present invention among the results of the treatment evaluation test It is a graph showing the decrease rate of the 11 bacterial bacteria causing disease.

4 and 5 of the results of the treatment evaluation test periodontal periodontal disease that occurs when taking a drug for three weeks or more recipe W (recipe W) prepared with the adjuvant composition for treatment and prevention of periodontal disease according to an embodiment of the present invention It is a graph showing the decrease rate of the 11 bacterial bacteria causing disease.

 6 and 7 are graphs showing the reduction rate of treponema denticola (Td) and parvimonas micra (Pm) of the results of the treatment evaluation test.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily practice the present invention. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art. Accordingly, embodiments of the invention may be modified in many different forms and should not be construed as limited to the embodiments set forth herein.

Throughout the specification of the present invention, when a part is said to "include" a certain component, it means to include other components rather than to exclude the other components unless otherwise stated.

Composition for treating and preventing periodontal disease using a natural extract according to an embodiment of the present invention (hereinafter, also referred to as 'composition for treating and preventing periodontal disease'), calcium, avocado extract, water-soluble propolis extract, corn extract, cinnamon It may be characterized by including the extract, thistle extract, vitamin C, vitamin B1, vitamin B2 and vitamin B6.

Three functions that are essential for the prevention and treatment of periodontal disease during dental care are bone formation, prevention and treatment of periodontal inflammation, and immunity enhancement.

Ingredients such as calcium, avocado extract, corn extract, and vitamin C have a strong bone-forming effect because they help to maintain and secure implant prostheses, helping to prevent and treat inflammation around the implant.

In addition, ingredients such as water soluble propolis extract, corn extract, cinnamon extract and vitamin C are effective in preventing or treating periodontitis because they strengthen the alveolar bone and periodontal ligament around teeth.

In addition, ingredients such as thistle extract, vitamin B1, vitamin B2 and vitamin B6, help to boost the body's overall immunity, and in particular, the components that help the liver function, which directly helps skin inflammation, especially vitamin B The group is excellent in boosting immunity because it helps nutritional and metabolic disorders and helps prevent and treat periodontal disease.

Therefore, the composition for treating and preventing periodontal disease using a natural extract according to an embodiment of the present invention is calcium, avocado extract, water-soluble propolis extract, corn extract, cinnamon extract, thistle extract, vitamin C, vitamin B1, vitamin B2 and Since it contains vitamin B6, it helps to form bones, prevents and treats periodontal inflammation, and because immunity is strengthened, it is necessary ingredients. By preparing the composition for treating and preventing periodontal disease, there is an advantage that the prevention and treatment effect of periodontal disease is superior to the existing periodontal disease treatment and prevention composition.

Composition for treating and preventing periodontal disease using a natural extract according to an embodiment of the present invention, calcium 35 to 60% by weight, avocado extract 0.5 to 7% by weight, water soluble propolis extract 5 to 25% by weight, corn extract 2 to 15% by weight, cinnamon extract 0.5-7%, thistle extract 2-15%, vitamin C 1-10%, vitamin B1 0.01-0.3%, vitamin B2 0.01-0.3% and vitamin B6 0.01-0.3% It may be characterized by comprising a%.

Calcium

Calcium is a component of the skeleton and teeth, 99% of the calcium content in the body is present here, and is also involved in maintaining the normal function of muscles and nerves. In addition, it regulates the contraction and relaxation of skeletal muscle and myocardial cells, and is a nutrient essential for the action of many enzymes associated with blood coagulation. Bone is composed of osteoblasts and osteoclasts (Osteoclast) cells, osteoblasts secrete collagen to form a support structure of bone, osteoblasts mature into osteocytes (Osteocytes) and cause calcification of bones. Therefore, the more calcium intake is maintained in the blood calcium concentration, calcium can be used for bone formation. In contrast, osteoclasts are stimulated by parathyroid hormones and are greatly activated when a diet is low in calcium, and the activated osteoclasts release calcium from the bone and enter the blood. Lowering calcium levels in the blood promotes the activation of osteoclasts. Since bone is formed and degraded by bone turnover (Bone remodeling) of osteoblasts and osteoclasts, calcium intake is important for bone formation and maintenance.

Calcium plays an important role in promoting bone formation and promoting metabolism and metabolic control hormones. Among them, calcium helps activate osteoblasts and inhibit osteoclasts when used as a composition for treating and preventing periodontal disease. Therefore, it is possible to increase the probability of success of implant and bone graft, and to promote bone formation and early fixation around the implant, thereby increasing the implant success rate. In other words, calcium is necessary for bone and teeth formation, plays an important role in maintaining nerve and muscle function, and is necessary for normal blood coagulation, so that it can be recovered and improved after bone transplantation, implant and periodontal surgery. It is an essential ingredient.

According to one embodiment of the present invention, calcium is preferably contained in 35 to 60% by weight relative to the total weight of the composition for treating and preventing periodontal disease using a natural extract. This is a content set according to the functional labeling functional dietary standard, according to the functional labeling functional food is known that the calcium content is 220 to 660mg per day.

According to one embodiment of the present invention, when the composition contains less than 35% by weight of calcium relative to the total weight of the composition for treating and preventing periodontal disease, the total weight of the composition for treating and preventing periodontal disease and the weight of components other than calcium Considered together, the calcium content contained in the composition for treating and preventing periodontal disease is lower than the lowest daily intake content according to the functional food functional labeling standards, there is a fear that the effect of calcium is not properly expressed. In addition, when calcium is included in excess of 60% by weight of the total weight of the composition for treating and preventing periodontal disease, considering the total weight of the composition for treating and preventing periodontal disease and the weight of components other than calcium, The content of calcium contained in the composition for treating and preventing periodontal disease is too excessive than the maximum content of daily intake according to the labeling standard, which may cause harmful effects due to the oversupply of calcium.

Excessive calcium intake causes Nephrolithiasis, Milk-alkali syndrome, which is caused by the ingestion of milk and absorbent alkaline gels for a long time, with or without hypercalcemia and alkalosis. It may cause adverse effects such as moderate alkalosis, abnormal serum phosphatase levels, severe renal failure with hypertension and calcification, and problems with calcium-mineral interactions.

Therefore, the calcium is preferably contained in 35 to 60% by weight relative to the total weight of the composition for the treatment and prevention of periodontal disease using natural extracts. In general, calcium may be used to prepare a composition for treating and preventing periodontal disease using a natural extract according to an embodiment of the present invention using calcium phosphate, but other types of calcium may be used.

2. Avocado Extract

Avocado (Persea americana Mill) is a fruit known to originate in Mexico and Central America and is widely consumed around the world. Avocados contain over 20 essential nutrients and a variety of phytochemicals. In other words, edible lipid content is about 25% and contains a large amount of monounsaturated fatty acids, carotenoids (Carotenoids), vitamin B, vitamin C, vitamin E, Terpenoids (Sitosterol), Persenone A (Persenone A) ), Persenone B, Phenolic, Protein, Fiber, Manganese, Phosphorus, Iron, Potassium and Folic Acid. 100 g of avocado contains 18.7 g of fat, 720 mg of potassium, 0.21 mg of vitamin B2, 61.90 mg of vitamin C, 71.80 mg of vitamin B6, 1.34 mg of vitamin E, and 5.30 mg of dietary fiber.

Antioxidant activity, Acetyl CoA carboxylase inhibition, and antimicrobial activity have been reported as avocados. In particular, the total polyphenol and flavonoid contents in methanol extracts of avocado pulp, seeds and husks were measured. The total polyphenol contents were 13.89 µg / mg for avocado pulp extract, 137.2 µg / mg for seed extract and 223.45 for shell extract. It was measured to be μg / mg, it was found that the polyphenol content in the seeds and shells is 10 to 20 times more than the pulp of avocado, having a high antioxidant capacity.

Avocado extract has an excellent polyphenol content and excellent antioxidant activity, and as the concentration of avocado increases the activity of glycoprotein (ALP) of the cell membrane increases osteoblasts. In addition, the avocado extract is effective in reducing the activity of TRAP enzyme (a cytochemical marker enzyme that can measure the degree of bone destruction in rats) without cytotoxicity and inhibits the differentiation of osteoclasts. For osteoblasts, the glycoprotein of the cell membrane While increasing (ALP) activity has been reported to be cytotoxic to osteoclasts, it has been demonstrated that avocado extracts have the effect of proliferating osteoblasts and inhibiting osteoclasts. In addition, avocado extract has the effect of helping the synthesis of collagen (Collagen) and proteoglycan (Proteoglycan). Therefore, it plays a very important role in strengthening bones by secreting collagen, proliferating osteoblasts forming bone support structures, and inhibiting osteoclasts leading to the release of calcium from the bone into the blood.

Avocado extract has osteoblast proliferation effect and osteoclast suppression effect, which helps bone formation. When used as a composition for treating and preventing periodontal disease, it has excellent effect on recovery and function after bone transplantation, implant and periodontal surgery. There is.

According to one embodiment of the present invention, the avocado extract is preferably contained in an amount of 0.5 to 7% by weight based on the total weight of the composition for treating and preventing periodontal disease using a natural extract.

According to one embodiment of the present invention, when the composition containing the avocado extract is less than 0.5% by weight relative to the total weight of the composition for treating and preventing periodontal disease, the total weight of the composition for treating and preventing periodontal disease and components other than avocado extract Considering the weight together, the content of avocado extract contained in the composition for the treatment and prevention of periodontal disease is too small, so the osteoblast proliferation and osteoclast inhibitory effects of the avocado extract are not properly expressed, so there is no possibility of adding meaning. have. In addition, when the avocado extract is included in excess of 7% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease, considering the total weight of the composition for treatment and prevention of periodontal disease and the weight of components other than avocado extract, periodontal disease The avocado extract contained in the therapeutic and prophylactic composition is so excessive that the amount of vitamin E contained in the avocado extract exceeds the daily intake limit, thus preventing the absorption of vitamin K in the body. It may reduce platelet aggregation, cause bleeding after surgery, and may cause adverse symptoms such as gastrointestinal disorders, muscle weakness, headaches and chronic fatigue.

Therefore, the avocado extract is preferably contained in an amount of 0.5 to 7% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease using natural extracts. The avocado extract may be prepared by a conventional method known in the art to which the present invention belongs, preferably washing and drying the avocado and pulverized and then using an extract extracted with an organic solvent such as methanol or ethanol have. In the present invention, the avocado was washed, dried and pulverized, and then avocado extract (Avocado Fruit 10: 1 Extract (Persea Gratissima)) extracted and extracted by adding ethanol was used.

3. Water Soluble Propolis Extract

Propolis is collected from the sap of trees, the bark of lost trees, and the surgery of flowers by bees, and is used for self-defense of honey bees or repair of honeycombs, mainly resins, beeswax, essential oils, pollen, various organic and mineral substances. It is composed of, and has been reported to be effective in antibacterial, antifungal, antiviral, antitumor, human immune reinforcement, wound healing as well as antioxidant activity. Various organic and mineral substances in propolis contain a large amount of more than 20 different flavonoids, and these flavonoids have been reported to exhibit strong antibacterial effects. Flavonoids of propolis are all aglycone different from flavonoids of other plants, and this compound is involved in the antibacterial action of propolis. In addition, since propolis does not have an antibacterial action against enterobacteriaceae, it is a very safe natural antimicrobial substance because it does not adversely affect enteric strains useful for the human body, that is, bifidobacteria or lactic acid bacteria even when administered orally.

Propolis has been reported to have excellent antioxidant and antibacterial effects in the oral cavity. Water-soluble flavonoid mixtures are effective in treating mucosal inflammation, and are effective in treating mucosal inflammation, such as clinically inhibiting gingival bleeding in patients with periodontal disease, and reduce caries morbidity due to the antibacterial and antioxidant effects of flavonoids. Flavonoids are excellent in reducing caries activity by reducing acid production in human oral saliva. For this reason, propolis has been actively developed as a component of oral health-related foods or products such as mouthwashes, periodontal disease treatments or supplements, toothpastes and the like.

Periodontal disease patients are suffering from inflammation occurring around various implants, especially after implantation, resulting in many problems. Patients take antibiotics to treat inflammation. Long-term use of antibiotics can cause resistance. Therefore, propolis is in the spotlight as an anti-inflammatory agent using a natural extract that can be taken in the long term because it does not develop resistance.

Propolis extract, which is used as a dietary supplement, is made from bees by mixing their secretions with resins obtained from plants, and removing wax, extracted with water or alcohol, or extracted with carbon dioxide. will be. Total flavonoids must be present in at least 1% and para-coumaric and cinnamic acid should be identified. In addition, diethylin glycol should not be detected.

In general, in order to use as a material for health functional food, raw materials of propolis are extracted and used as an organic solvent, but in rare cases, side effects such as allergies may occur due to the organic solvent. In addition, since propolis has been reported to cause inflammation or ulceration on mucous membranes and cause hypersensitivity reactions such as asthma, the composition for treating and preventing periodontal disease of the present invention is water-soluble to prevent allergic reactions and inflammation or ulceration of mucous membranes. Propolis extract was used.

According to one embodiment of the present invention, the water-soluble propolis extract is preferably contained in 5 to 25% by weight relative to the total weight of the composition for treating and preventing periodontal disease using a natural extract. This is the amount of water-soluble propolis extract is set in consideration of the intake in the human application test confirmed the functionality.

As the total flavonoid, 16 to 17 mg is most preferred as the daily intake. Since the propolis extract used as a dietary supplement should contain at least 1% of total flavonoids, the water-soluble propolis extract is preferably 160 to 200 mg in daily intake.

According to one embodiment of the invention, when the composition containing the water-soluble propolis extract less than 5% by weight based on the total weight of the composition for treating and preventing periodontal disease, the total weight and water-soluble propolis extract for treating and preventing periodontal disease Considering the weight of other ingredients together, the water-soluble propolis extract contained in the composition for treating and preventing periodontal disease is less than the minimum daily intake set in consideration of the intake in the functional application test. Therefore, there is a fear that the antioxidant action and the antibacterial action effect of the water-soluble propolis extract are not properly expressed. In addition, when the water-soluble propolis extract contains more than 25% by weight of the total weight of the composition for treating and preventing periodontal disease, the total weight of the composition for treating and preventing periodontal disease and the weight of components other than the water-soluble propolis extract may be considered together. When the amount of water-soluble propolis extract contained in the composition for treating and preventing periodontal disease is too excessive than the highest daily intake of the functional food functional labeling standard, it is not common, but it is a rare side effect of propolis. It may cause an allergic reaction and inflammation or ulceration of the mucous membrane.

Therefore, the water-soluble propolis extract is preferably contained in 5 to 25% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease using natural extracts. The water-soluble propolis extract may be prepared by a conventional method known in the art to which the present invention belongs, preferably by applying heat to the raw raw raw propolis to remove the bewax (beewax), then extracted into the spirit Extracted extracts may be used. In the present invention, a water-soluble propolis extract (Water Soluble Bee Propolis Ext. 70%) prepared by applying heat to a raw propolis raw material to remove non-wax, and then adding alcohol, was used.

4. Corn Extract

Corn extract (corn unchecked quantitative extract, Zea Mays L.) is currently used as a component of health functional foods and products aimed at preventing periodontal disease or preventing periodontal disease. This is due to the fact that Zea Mays L. (hereinafter referred to as 'ZML'), an unchecked quantitative extract from corn, was found to be effective in improving purulent and periodontitis in 1958. It has been shown in the experiment that when taken, it has the effect of preventing and regenerating alveolar bone absorption and destruction of periodontal ligament. In addition, as a result of the reduction of dental rhythm and alveolar bone resorption in patients with periodontitis, ZML was reported to stop inflammatory degradation of bone in about 80% after topical treatment. It has been found to be effective in alleviating periodontal disease.

Corn extract is composed mainly of phytosterol consisting of beta-Sitosterol, Stigmasterol and Ergosterol, Squlaene, a hydrocarbon, Carotene and Tocopherol (liposoluble vitamins). Tocopherol). In particular, the composition for the treatment and prevention of periodontal disease according to an embodiment of the present invention uses that containing 10% or more of beta-sitosterol.

Beta-sitosterol is the most widely distributed plant sterol, a precursor of vitamin D, or provitamin D, which promotes absorption of calcium, promotes alveolar bone formation, regenerates periodontal ligament fibers, Excellent function of normalization and anti-inflammatory action. Beta-sitosterol promotes osteoblast production by activating the endoplasmic reticulum of osteoblasts, promotes the regeneration of the collapsed alveolar bone, and prevents the absorption of the alveolar bone, thereby strengthening the gums. In addition, it is excellent in regenerating damaged periodontal ligament fibers and preventing abnormal shaking of teeth. In addition, because it has an excellent function of restoring damaged epithelial cells, and has excellent anti-inflammatory action that can strengthen gums and resist bacterial infection, the composition for treating and preventing periodontal disease according to an embodiment of the present invention is beta-sitosterol. It is important to use corn extracts containing more than 10%.

According to one embodiment of the present invention, the corn extract is preferably contained by containing 2 to 15% by weight relative to the total weight of the composition for the treatment and prevention of periodontal disease using natural extracts. This is a periodontal disease is determined based on the content of the corn extract listed in the general medicine, the daily intake is preferably 80 to 120mg.

According to an embodiment of the present invention, when the corn extract is contained in an amount of less than 2% by weight based on the total weight of the composition for treating and preventing periodontal disease, the total weight of the composition for treating and preventing periodontal disease and components other than corn extract Considering the weight together, the amount of corn extract contained in the composition for treating and preventing periodontal disease is too small, activating osteoblast vesicles of corn extract, and promoting calcium absorption as a precursor of vitamin D, alveolar bone Effects such as promoting regeneration and periodontal ligament fiber regeneration are not properly expressed, there is a fear that it does not add meaning. In addition, when the corn extract exceeds 15% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease, considering the total weight of the composition for the treatment and prevention of periodontal disease and the weight of components other than corn extract, periodontal disease The amount of corn extract contained in the therapeutic and prophylactic composition is so excessive that the production cost may increase, resulting in a decrease in productivity, which may affect the component content of other periodontal disease treatment and prophylactic compositions. not.

Therefore, the corn extract is preferably contained by containing 2 to 15% by weight relative to the total weight of the composition for the treatment and prevention of periodontal disease using natural extracts. The corn extract may be prepared by a conventional method known in the art to which the present invention belongs, preferably washing the corn and cornbeard together, dried and pulverized, ethanol or water is added to the pulverized ground After the extraction can be used to extract the extract can be used, corn unchecked quantitative extract commonly used in the manufacture of periodontal disease medicines. In the present invention, the corn extract (GMO Free Corn Silk with 10% Beta Sitosterol (Zea Mays)) was prepared by washing corn, drying and pulverizing, and adding ethanol to the pulverized powder. .

5. Cinnamon Extract

Cinnamon is a dry, evergreen tropical cinnamon tree belonging to the family Lauraceae. Herbal medicine has been used for the treatment of headache, fever, nervousness, and pain. The main ingredients of cinnamon are essential oils called cinnamon oils, which are Cinnamic aldehyde, Campen cineol, Linalool, Eugenol, Sugar, Fat, Phosphorus, Iron, Because it contains ingredients such as vitamin A, vitamin B1, and vitamin B2, it is widely used as a fragrance component in beverages, chewing gums, toothpaste and cosmetics, and bad breath remover. have. In addition, it stimulates the gastrointestinal mucosa, stimulates secretion, suppresses spasms of pain in the stomach, promotes the movement of the gastrointestinal tract, and releases gas and improves absorption. In addition, cinnamon stimulates nerves, promotes blood circulation, warms the body, and has an antiseptic effect of inhibiting abnormal fermentation in the intestine. Cinnamic aldehydes of cinnamon have a function of persistent peripheral vasodilation (hypertension) and platelet aggregation inhibition, and effects such as sweating and clay. It is widely used for colds, analgesic, and dysmenorrhea, and cinnamon extract is also excellent in antimicrobial activity to suppress food corruption.

Cinnamon extract can be used as a natural antibiotic and bad breath remover because of its unique flavor, antioxidant and anti-inflammatory properties. In particular, since it is excellent in inhibiting inflammation by acting as a natural antibiotic with water-soluble propolis which is a component of the composition for treating and preventing periodontal disease of the present invention, it is an important component in the composition for treating and preventing periodontal disease.

According to one embodiment of the invention, the cinnamon extract is preferably contained in 0.5 to 7% by weight relative to the total weight of the composition for treating and preventing periodontal disease using a natural extract.

According to one embodiment of the present invention, when the cinnamon extract is contained in an amount of less than 0.5% by weight based on the total weight of the composition for treating and preventing periodontal disease, the total weight of the composition for treating and preventing periodontal disease and components other than cinnamon extract Considering the weight together, the content of cinnamon extract in the composition for the treatment and prevention of periodontal disease is too small, and the antioxidant and anti-inflammatory effects of cinnamon extract may not be properly expressed, which may prevent it from functioning as a natural antibiotic. . In addition, when the cinnamon extract is included in excess of 7% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease, considering the total weight of the composition for the treatment and prevention of periodontal disease and the weight of components other than cinnamon extract, periodontal disease The amount of cinnamon extract contained in the therapeutic and prophylactic composition is so excessive that it can be fatal to a person with a fever or a lot of heat in the body, there is a fear that productivity may decrease due to increased production cost, and treatment and prevention of other periodontal disease It is not preferable because it may affect the component content of the composition.

Therefore, the cinnamon extract is preferably contained in an amount of 0.5 to 7% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease using natural extracts. The cinnamon extract may be prepared by a conventional method known in the art to which the present invention belongs, preferably extract the water by grinding the cinnamon, it can be used through the process of filtration, concentration and purification have. In the present invention, after adding water to the cinnamon is repeated three times the extraction process to extract for 2 hours at a temperature of 75 to 85 ℃, after the filtration process, concentrated under reduced pressure and purified at a temperature of 75 to 85 ℃ The cinnamon extract (Cinnamon Bark 2: 1 Extract (Cinnamomum Zeylanicum)) prepared by drying was purchased and used.

6. Thistle Extract

Milk Thistle is an asteraceae, scientific name is Silybum marianum (L.) Gaertn, tinnitus is Carduus silybum, and fruit and seeds are used for food. Traditionally, herbalists have used thistle (milk thistle) seeds to protect the liver, such as chronic epilepsy, and in Europe, major countries such as Silymarin of thistle (milk thistle) protect liver cells from free radicals. Because it is known to be used as a liver supplement or a dietary supplement.

Thistle (milk thistle) extract is produced by pulverizing thistle milk (milk thistle), extracted by alcohol, filtration, concentration and purification, 320 ~ 660mg of functional ingredient silymarin (Silymarin) It is a raw material containing about / g. Silymarin consists of four isomers: Silybin (A, B), Silychristin, Silydianin, and Isosilybin (A, B).

Thistle (milk thistle) contains a component called Silymarin (Silymarin) is known to be effective in improving the function of the liver. Since the antioxidant activity is 10 times higher than vitamin E, glutathione secretion is increased to facilitate liver detoxification, which is effective in regenerating liver tissue, which helps restore liver function. In addition, silymarin (Silymarin), which is extracted from the seeds of thistle, has the effect of inhibiting the proliferation of cancer cells, and the research results are also effective in preventing and treating inflammation or liver damage, which is a side effect of cancer patients undergoing chemotherapy. Turned out to be excellent.

That is, the milk thistle silymarin (Silymarin) component is used as a liver supplement and a dietary supplement because it has an excellent antioxidant activity, such as protecting the liver cells from free radicals, and is beneficial for liver health. The recovery of liver function is very important in the treatment of periodontal disease because of the direct relationship between skin and immunity. Therefore, the milk thistle silymarin (Silymarin) component is effective in restoring liver function, it is an essential ingredient for recovery and function after bone graft, implant and periodontal surgery, so the composition for the treatment and prevention of periodontal disease Essential ingredient in the.

According to one embodiment of the present invention, the thistle extract is preferably contained in an amount of 5 to 15% by weight relative to the total weight of the composition for treating and preventing periodontal disease using natural extracts. This is the content set according to the functional labeling standards of functional foods.

In Korea, thistle seeds and berries are used as food ingredients, and thistle extract is also used as a general medicine. Thistle extract is used abroad as a dietary supplement and medicine. It is listed as a food in the Prehensine Database and as a Dietary Supplement in the USP-NP. In clinical trials conducted with silymarin 210-260 mg / day, no adverse effects were reported. The WHO monograph recommends 200-400 mg daily intake of silymarin, but functional health foods are considered in consideration of safety as a dietary supplement. When the content is set according to the standard, the daily intake is preferably 80 to 240 mg based on silymarin.

According to one embodiment of the present invention, when the composition of the composition for treating and preventing periodontal disease is less than 5% by weight based on the total weight of the composition for treating and preventing periodontal disease, the total weight of the composition for treating and preventing periodontal disease and components other than thistle extract Considering the weight together, the amount of thistle extract contained in the composition for treating and preventing periodontal disease is too small, compared to the minimum daily intake set in consideration of the content set according to the functional food functional labeling standard. There is a fear that the function recovery effect is not properly expressed, so there is no meaning to add. In addition, if thistle extract contains more than 15% by weight relative to the total weight of the composition for the treatment and prevention of periodontal disease, when considering the total weight of the composition for the treatment and prevention of periodontal disease and the weight of components other than thistle extract, health function The amount of thistle extract contained in the composition for the treatment and prevention of periodontal disease is too much higher than the maximum daily intake set in consideration of the content set according to the food functional labeling criteria, and thus, harmful symptoms such as diarrhea, headache and joint pain may occur. There is concern.

Therefore, the thistle extract is preferably contained by containing 5 to 15% by weight relative to the total weight of the composition for the treatment and prevention of periodontal disease using natural extracts. The thistle extract may be prepared by a conventional method known in the art to which the present invention belongs, preferably, thistle is pulverized and extracted with alcohol, and then the extract prepared through the process of filtration, concentration and purification to use Can be. In the present invention, thistle was pulverized and extracted by the addition of alcohol, and then the extracted extract was filtered and concentrated and stagnant thistle extract (Milk Thistle 80% Silymarin (Silybum marianum)) purchased and used.

7. Vitamin C

Vitamin C is a six-carbon lactone, and plants and most animals can synthesize vitamin C in the body, but humans, primates, guinea pigs, large bats, and some species of birds and fish can synthesize it. Therefore, it must be in the form of food. Vitamin C contains both reduced ascorbic acid and oxidized dehydroascorbic acid, which can be converted. In fact, there are two types of food we eat.

Vitamin C has the function of forming connective tissue, which is used as a coenzyme of various enzymatic reactions in vivo, and is involved in the synthesis of collagen, a major protein constituting connective tissue such as skin, skeleton, blood vessels and cartilage. When the amino acids proline and lysine are hydrated to form hydroxyproline and hydroxylysine, vitamin C plays a role in reducing the iron content of the hydroxylase. To maintain collagen tissue.

The electron donating function of vitamin C induces antioxidant activity to remove free radicals such as reactive oxygen species (ROS) and reactive nitrogen species (RNS). In particular, tissues susceptible to damage from free radicals, such as eyeballs, neutrophils, and semen, are known to have a relatively high content of vitamin C. In addition, it prevents the onset of lipid peroxidation such as low density lipoprotein (LDL). Get involved. In addition, the radicals of vitamin C have a relatively long half-life of 5 to 10 seconds compared to other antioxidants, and thus are recognized as stable antioxidants.

Vitamin C is also known to be excellent in iron absorption effects, such as promoting the absorption of vegetable iron that is not bound to hemoglobin by converting iron to a reduced form in the small intestine. In other words, vitamin C is necessary for the formation and maintenance of connective tissue, and it is effective for iron absorption and protects cells from harmful oxygen. Therefore, vitamin C is recovered and improved after bone transplantation, implant and periodontal surgery. Essential ingredient for

According to one embodiment of the present invention, vitamin C is preferably contained in an amount of 1 to 10% by weight based on the total weight of the composition for treating and preventing periodontal disease using a natural extract. This is a content set according to the recommended daily intake of adult men and women.

The average requirement for vitamin C in men and women is 75 mg / day and the recommended intake is 100 mg / day. The recommended intake is set based on the amount of urine excretion while keeping leukocytes' vitamin C levels to a maximum.

When the composition of the composition for treating and preventing periodontal disease is less than 1% by weight of the total weight of the composition, when considering the total weight of the composition for treating and preventing periodontal disease and the weight of components other than vitamin C, The content of vitamin C contained in the composition for the treatment and prevention of periodontal disease is less than the average required amount of, there is no meaning to add vitamin C, there is a fear that the effect of vitamin C is not properly expressed. In addition, when vitamin C is included in an amount of more than 10% by weight based on the total weight of the composition for treating and preventing periodontal disease, when considering the total weight of the composition for treating and preventing periodontal disease and the weight of the components other than vitamin C, The amount of vitamin C contained in the composition for the treatment and prevention of periodontal disease is too excessive than the recommended intake of, may cause problems due to the excessive supply of vitamin C.

When supplemental foods in which excess vitamin C is added, it is known to mainly cause gastrointestinal symptoms such as nausea, vomiting, bloating, abdominal pain and diarrhea. In addition, fishery excretion and nephrolithiasis, increased uric acid excretion, excessive iron absorption, and vitamin B12 levels have been reported. In pregnant women, excessive vitamin C ingestion has been reported to cause vitamin C dependence in newborns. In animal and in vitro studies, vitamin C is pro-Oxidant and may have adverse effects. It is reported that it is possible.

Therefore, the vitamin C is preferably contained in an amount of 1 to 10% by weight based on the total weight of the composition for treating and preventing periodontal disease using a natural extract.

8. Vitamin B1

Vitamin B1 (Thiamin) is a hexagonal ring (Pyrimidine) containing nitrogen in the center and sulfur pentagonal ring (Thiazole) connected by a methylene bridge.

Pork, sunflower seeds, brewer's yeast, soybeans, potato wheat germ, whole grains and fortified grains, peas, asparagus, meat guts, peanuts and other seeds, mushrooms and watermelons are good sources of vitamin B1. As such, there are many foods containing vitamin B1, but in general, the content is low, so it is desirable to eat a variety of foods.

Thiamin pyrophosphate (TPP), a coenzyme form of vitamin B1, has two phosphate groups, including pyruvate, alpha-ketoglutarate, and branched-chain keto acids. It serves to catalyze the decarboxylation of the same alpha-keto acids. It also catalyzes the transketolation reaction between hexose phosphates and pentose phosphates. Transketolase, which is required for ketotransition reactions, is an enzyme that requires TPP to produce pentose sugar from glucose, which is hexose, in the Pentose phosphate pathway. Vitamin B1 is therefore required for the metabolism of carbohydrates and branched amino acids (leucine, isoleucine, valine). That is, vitamin B1 is involved in energy metabolism, so the required amount of vitamin B1 is highly correlated with energy consumption.

Since vitamin B1 is required for carbohydrate and energy metabolism, when used as a composition for the treatment and prevention of periodontal disease, it can solve the weakness of carbohydrate metabolism that should be chewed and digested by periodontal disease patients. It performs an effective function in periodontal regeneration of patients with chronic periodontal disease.

According to one embodiment of the present invention, vitamin B1 is preferably contained in an amount of 0.01 to 0.3% by weight relative to the total weight of the composition for treating and preventing periodontal disease using a natural extract. This is a content set according to the recommended daily intake of adult men and women.

The average vitamin B1 requirement of Korean adults is 1.0mg / day for males and 0.9mg / day for females. The recommended intake is 120% of the average intake, 1.2mg / day for males and 1.1mg / day for females. The indicator used in setting the recommended intake is the red blood cell's transketolase activity. The activity of this enzyme is measured by comparing the activity with and without the addition of the coenzyme of vitamin B1 after the destruction of blood cells.

When vitamin B1 is contained in an amount of less than 0.01% by weight based on the total weight of the composition for treating and preventing periodontal disease, when considering the total weight of the composition for treating and preventing periodontal disease and the weight of components other than vitamin B1, The content of vitamin B1 contained in the composition for the treatment and prevention of periodontal disease is less than the average required amount of, it does not mean adding vitamin B1, there is a fear that the effect of vitamin B1 is not properly expressed. In addition, when the total weight of the composition for the treatment and prevention of periodontal disease exceeds 0.3% by weight, when considering the total weight of the composition for the treatment and prevention of periodontal disease and components other than vitamin B1, adult men and women The amount of vitamin B1 contained in the composition for treating and preventing periodontal disease is too excessive than the recommended intake of may cause problems due to oversupply.

Naturally, human body toxicity of vitamin B1 in food is very low, but there have been reports of toxicity when high concentrations of vitamin B1 are consumed for therapeutic purposes. High levels of vitamin B1 usually cause central nervous system-related symptoms such as headaches, nausea, anxiety, insomnia, tachycardia, and lack of energy.

Therefore, the vitamin B1 is preferably contained in an amount of 0.01 to 0.3% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease using a natural extract.

9. Vitamin B2

Vitamin B2 (Riboflavin) is a three-hexagonal ring molecule, usually present in the form of Flavin adenine dinucleotide (FAD) and Flavin mononucleotide (FMN) in the biological world, with very few free vitamin B2. Vitamin B2 is relatively stable against acid, heat and oxidation, but weak against alkali and light. Animal foods such as liver, meat, chicken and fish and dairy products are good sources of vitamin B2, and soybeans, green vegetables, grains and eggs are also used as sources of vitamin B2.

Vitamin B2 has excellent caloric metabolism. Coenzymes of vitamin B2 are involved in various enzymatic reactions, especially in caloric metabolism. Various types of vitamin B2 enzymes are used for the metabolism of carbohydrates, fats and amino acids, dehydrogenation, hydroxylation, oxidative decarboxylation, dioxygenation and oxygen peroxide. It is essential for the reduction reaction.

Deficiency of vitamin B2 causes deficiency such as stomatitis, stomatitis, and periarthritis in the oral cavity, oral or rash of the nose and mouth, seborrheic dermatitis of the vulva, ocular hyperemia, photophobia, premature cataract, and anemia. . Therefore, vitamin B2 deficiency causes various symptoms for the oral cavity, and proper intake of vitamin B2 is effective for periodontal disease and chronic periodontal disease because vitamin B2 has an excellent function in energy generation and metabolism. In particular, the composition for the treatment and prevention of periodontal disease of the present invention there is a concern that the metabolism may not be smooth due to the high content of plant extracts, that is, fat-soluble components, the treatment and prevention of periodontal disease because the metabolism can be smoothed by the addition of vitamin B2 It is an essential ingredient to enhance the effect of the composition.

According to one embodiment of the present invention, vitamin B2 is preferably contained in an amount of 0.01 to 0.3% by weight relative to the total weight of the composition for treating and preventing periodontal disease using a natural extract. This is a content set according to the recommended daily intake of adult men and women.

The average amount of vitamin B2 can be set based on the activity of glutathione reductase in red blood cells and the amount of vitamin B2 excretion in urine.The average amount of vitamin B2 in Korean adults is 1.3 mg / day for men and 1.0 mg / day for women. The recommended intake is 120% of the average intake for men 1.5mg / day and women 1.2mg / day.

When the composition of the composition for treating and preventing periodontal disease is less than 0.01% by weight of vitamin B2, the total weight of the composition for treating and preventing periodontal disease and the weight of components other than vitamin B2 are considered. Since vitamin B2 content in the composition for the treatment and prevention of periodontal disease is less than the average required amount of vitamin B2, it may not effectively affect calorie metabolism (the metabolic pathways of carbohydrates, fats and amino acids). There is no meaning or the effect of vitamin B2 may not be properly expressed. In addition, when the total weight of the composition for the treatment and prophylaxis of periodontal disease exceeds 0.3% by weight, when considering the total weight of the composition for the treatment and prevention of periodontal disease and components other than vitamin B2, adult men and women Vitamin B2 contained in the composition for the treatment and prevention of periodontal disease is too high than the recommended intake of, so the human body's ability to absorb vitamin B2 in the gastrointestinal tract is limited. Because of the sudden increase in the excretion of vitamin B2 yellow urine may be excreted.

Therefore, the vitamin B2 is preferably contained in an amount of 0.01 to 0.3% by weight relative to the total weight of the composition for treating and preventing periodontal disease using a natural extract.

10. Vitamin B6

Vitamin B6 is present in foods in pyridoxine (Pyridoxine, PN), pyridoxal (PL), pyridoxamine (Pyridoaxamine, PM) or in their respective phosphorylated forms (PLP, PNP, PMP). In animal foods, PLP, PMP, and protein are present in a combined form. In plant foods, PN, PNP, and sugar are present in a combined form. Coenzyme forms of vitamin B6 are PLP and PMP, in particular PLP is a form of biological activity is very high. The free form of vitamin B6 varies with acidity, but is relatively unstable and easily denatured.

Sources of vitamin B6 include animal foods such as fish, pork, chicken, eggs and animal intestines (liver and kidneys), as well as brown rice, soybeans, and oats. Dairy products are relatively low in vitamin B6.

Vitamin B6 is a necessary ingredient for the use of proteins and amino acids. PLP is a coenzyme of enzymes involved in the metabolism of about 100 kinds of amino acids such as aminotransferase, decarboxylase and stereoisase. For example, PLP is required for the reaction to synthesize non-essential amino acids, and if there is no PLP, all amino acids become essential amino acids and must be supplied by meal. In addition, PLP is a stereoisomer and acts on the interconversion of D-type and L-type amino acids. Since only L-type amino acids are used when protein is synthesized in the human body, the action of PLP is important. In addition, vitamin B6 also acts as a coenzyme of the catabolism of the homocysteine and Phosphorylase (Phosphorylase) reaction, it is also an essential component in maintaining the homocysteine level of blood normal.

Clinical deficiency symptoms of vitamin B6 include interstitial lethargy, dermatitis, stomatitis, cleft lip, pharyngitis, depression, severity abnormalities, and in animals, anemia, fatty liver, abnormal use of stored energy in the body has also been reported. Therefore, vitamin B6 is a necessary ingredient for the prevention and treatment of oral diseases such as stomatitis, stomatitis, and sulfitis. In particular, the liver function is restored by working with thistle (milk thistle) extract of the composition for the treatment and prevention of periodontal disease of the present invention, the vitamin complex of vitamin B1, vitamin B2 and vitamin B6 is milk thistle (thistle extract) ) In parallel with the metabolism facilitates to enhance immunity and to treat and prevent periodontal disease is excellent.

According to one embodiment of the present invention, vitamin B6 is preferably contained in an amount of 0.01 to 0.3% by weight relative to the total weight of the composition for treating and preventing periodontal disease using a natural extract. This is set according to the recommended daily intake of adult men and women.

The average amount of vitamin B6 in Korean adults is 1.3mg / day for men and 1.2mg / day for women, and the recommended intake is 120mg of average intake, 1.5mg / day for men and 1.4mg / day for women.

When the composition of the composition for the treatment and prevention of periodontal disease contains less than 0.01% by weight of vitamin B6, when considering the total weight of the composition for treating and preventing periodontal disease and the weight of components other than vitamin B6, The content of vitamin B6 contained in the composition for the treatment and prevention of periodontal disease is less than the average required amount of, it does not mean adding vitamin B6, there is a fear that the effect of vitamin B6 is not properly expressed. In addition, when the total weight of the composition for treating and preventing periodontal disease exceeds 0.3% by weight, when considering the total weight of the composition for treating and preventing periodontal disease and the weight of components other than vitamin B6, adult men and women The amount of vitamin B6 contained in the composition for the treatment and prevention of periodontal disease is too excessive than the recommended intake of may cause problems due to oversupply.

Vitamin B6 excess from food has not been reported, but it has been reported that sensory neuropathy and skin lesions may occur when a large amount of the drug is taken for the purpose of treating diseases such as premenstrual syndrome. Ingestion of high doses of vitamin B6 can cause nerve damage, tingling limbs, staggering steps, loss of sensation around the mouth, and loss of muscle coordination.

Therefore, the vitamin B6 is preferably contained in an amount of 0.01 to 0.3% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease using a natural extract.

Therefore, the composition for treating and preventing periodontal disease using a natural extract according to an embodiment of the present invention, calcium 35 to 60% by weight, avocado extract 0.5 to 7% by weight, water soluble propolis extract 5 to 25% by weight, corn extract 2 to 15% by weight, cinnamon extract 0.5 to 7% by weight, thistle extract 2 to 15% by weight, vitamin C 1 to 10% by weight, vitamin B1 0.01 to 0.3% by weight, vitamin B2 0.01 to 0.3% by weight and vitamin B6 0.01 to Most preferably, it comprises 0.3% by weight.

According to one embodiment of the present invention, to the composition for the treatment and prevention of periodontal disease using the natural extract, by adding any one or more from the additive group consisting of silicon dioxide, magnesium stearate, crystalline cellulose and a color coating agent in the form of a tablet It may be characterized in that it is manufactured as.

This is to add the components of the additive group to prepare the composition for the treatment and prevention of periodontal disease using the natural extract in the form of tablets for easy ingestion, added silicon dioxide, magnesium stearate, crystalline cellulose and color coating agent Components of the additive group consisting of does not cause interaction with each component of the composition for treating and preventing periodontal disease using the natural extract, it is preferable that the composition is composed of components that do not affect the effective aspect.

In addition, according to an embodiment of the present invention, 0.1 to 1 parts by weight of silicon dioxide, 0.1 to 1.5 parts by weight of magnesium stearate, crystals, corresponding to an additive group based on 100 parts by weight of the composition for treating and preventing periodontal disease using the natural extract 12 to 26 parts by weight of cellulose and 1 to 5 parts by weight of the color coating agent may be added to form a tablet formulation.

The content of the components of the additive group consisting of silicon dioxide, magnesium stearate, crystalline cellulose and a color coating agent is suitable for preparing a periodontal disease treatment and prevention composition using a natural extract in a tablet form, and treatment of periodontal disease using a natural extract. And it can be set to an optimized content so as not to affect the effect of the composition for prevention. Thus, the ratio of 0.1 to 1 parts by weight of silicon dioxide, 0.1 to 1.5 parts by weight of magnesium stearate, 12 to 26 parts by weight of crystalline cellulose and 1 to 5 parts by weight of the color coating agent based on 100 parts by weight of the composition for treating and preventing periodontal disease using a natural extract. It is preferred to prepare further in the form of tablet form by further addition.

EXAMPLE

To prepare a composition for the treatment and prevention of periodontal disease using a natural extract according to an embodiment of the present invention.

590 mg of calcium, 35 mg of avocado extract, 175 mg of water-soluble propolis, 100 mg of corn extract, 35 mg of cinnamon extract, 100 mg of thistle extract, 66 mg of vitamin C, 1.2 mg of vitamin B1, 1.4 mg of vitamin B2 and 1.5 mg of vitamin B6 After mixing the natural extracts of the additives, silicon dioxide 2 mg, magnesium stearate 10 mg, crystalline cellulose 280 mg and 42 mg of color coating agent is further added to the tablet form to treat and prevent periodontal disease using natural extracts. Prepare the composition.

[Poll]

To investigate and present the application range of the composition for treating and preventing periodontal disease according to an embodiment of the present invention, the effects and usage of purchased patients, and to prepare a basis for introducing the composition for treating and preventing periodontal disease in a hospital. An investigation was conducted. The periodontal disease adjuvant composition using the natural extract prepared as in Example was prepared in a tablet form of the drug recipe W. (Recipe W) to be administered to the study subjects, and expressed in the recipe W. (Recipe W) in the questionnaire and the questionnaire results. It is.

1. Questionnaire design

1 is a questionnaire of a survey conducted to grasp the effects and use status of purchase patients with the composition for the treatment and prevention of periodontal disease according to an embodiment of the present invention.

The questionnaire items in this study were classified by patient symptom, non-answered phone call, effectiveness of recipe W after 1 month of receiving dose, status of effect compared to other products, classification by period, periodontal condition improvement, periodontal The questionnaire consists of 9 questions such as improvement by symptom, repurchase status for one month, and intention of continuous taking. About 10 selected dentists at Seoul National University, Hallym University, and Pusan National University evaluated the selected items and evaluated the suitability of the selected items.

2. Selection of research subjects and investigation

The purpose of this study was to inform patients of the purpose of this study to understand the effectiveness and scope of recipe W. It was conducted with the consent of patients under the premise that it was not used for the purpose of this study and statistical purposes.

The subjects underwent a telephone survey of 356 patients who purchased Recipe W at the clinic from April 9 to May 8, 2014. The number of males and females of recipe W is 251 females and 105 males.The ages are 2 in 20s, 4 in 30s, 35 in 50s, 127 in 50s, 116 in 60s, and 70s. It is distributed in 65, 80 and 7 members.

Symptoms included 248 patients with peri-implantitis, 80 chronic periodontal diseases, 28 general periodontal diseases, 18 in the implant plan, and 6 patients with osteoporosis medication.

In this study, a complete survey was conducted to provide basic data. The telephone survey used Cpm Changwon Branch (service agency) for objectivity, and the telephone survey was conducted for five days from June 9 to 13, 2014, and from 11 am to 1 pm, From 2 pm to 5 pm, the maximum number of researchers was allowed to participate. If the user did not answer the phone more than 3 times with a time difference, it was not answered. Of the 356 subjects, 251 responded and analyzed the results on a total of 251 subjects.

3. Research Results

3-1. General Characteristics of Survey Subjects

A total of 251 respondents purchased the Recipe W periodontal disease adjuvant from a dental clinic. The most common symptom was peri-implantitis (182 patients, 70%), and chronic periodontal disease (59 patients, 23%). Ten patients (4%), seven patients planning implants (3%), and one patient replacing osteoporosis drugs (0%).

3-2. recipe  Duration and Effect of W

60.2% (151) of the total respondents were the most patients who took more than 1 month, 22.7% (57 people) and 17.1% (43) others.

Of the 251 surveyed, 61% (153) said they worked, and 39% (98) said they didn't work. It has some effect in detail whether it is effective 35.1% (88 people), big effect 25.9% (65 people), preventive level 14.3% (36 people), not taking well 12% (30 people) 9.6% (24 people) with no feelings, no effect 3.2% (8 people).

A total of 43 patients answered that they had taken other products as a treatment for periodontal disease. 49% (21 people) of respondents answered that there was some effect, 44% (19 people) were effective, and 7% (3 people) had no effect.

3-3. Periodontal condition improvement

86% (237) of the total respondents reported that their periodontal condition improved, and 14% (37) answered that they did not improve.

The most common symptom was improvement of tooth agitation and gum weakness (71 cases), improvement of pain and swelling of 61 cases, improvement of bleeding when brushing teeth, improvement of ache 42, and improvement of bad breath.

4. Willingness to Repurchase

During the 30 days from April 9 to May 8, 2014, the actual repurchase rate of 356 patients was 22% (99 people). Of the 251 telephone respondents, 56.9% (143) wanted to repurchase, 36.3% (91) answered that they were considering repurchase, and 6.7% (17) did not want to repurchase. The reason for not wanting to repurchase was 7 people who thought it was ineffective, 7 patients who answered that the fragrance of propolis was strong, and 3 who stopped taking after cure.

Therefore, 251 of the 356 subjects responded to the telephone questionnaire. According to the symptom of receiving Recipe W, 251 respondents showed peri-implantitis 70% (182), chronic periodontal disease 23% (59), periodontal disease 4% (10), and 3% (7) of the implant plan. 0% (1 person) is a substitute for osteoporosis drugs, which is biased for patients with peri-implant peritonitis, but the number of patients is considered as a basis for introducing periodontal disease supplements. In addition, the results of the survey can be considered as the final result, considering that the proportion of symptom by 356 subjects and that of symptom by 251 respondents are almost similar. According to the results of Recipe W, the patients who took it continuously seemed to be effective enough. Among the 173 patients who took more than a month, 22% (99) of the actual repurchase patients were repurchased. 56.9% (143 patients) of the patients desired showed the importance of taking the patient.

According to the results of Recipe W, 61% (153) of respondents said it was effective, showing similarity among 143 respondents who wanted to repurchase. This is the basic part of the constant dose to determine the effect. Of the 39% (98) who reported no effect, there were 30 patients who did not take it properly, including 36 patients taking prophylactic measures, 24 patients who felt poorly, and 8 patients who said they were ineffective. Given that, taking the correct dose is an important part. A survey of the effectiveness of other products shows that 43 (17%) of respondents have tried other products, which suggests that there may be a large number of patients who rely on drugs without periodontal care in dental hospitals and clinics. As a result of the survey compared to other products, 93% (40 people) of the respondents answered that the effect was large or large. We believe it is more significant than other products in the market. In the survey of patients with periodontal improvement, 86% (237 cases) improved and 14% (37 cases) did not. The conclusions that can be drawn from this survey suggest that there may be an improvement of 86% in about two months of Recipe W, and a significant improvement over longer periods. According to the symptom improvement questionnaire, there were 71 cases of improvement in tooth agitation and gum weakness, 61 cases of pain and swelling improvement, 59 cases of improvement in bleeding when brushing teeth, 42 cases of improvement in bad teeth, and 4 cases of bad breath improvement. However, there is a significant difference in terms of bad breath between the patient's feelings and the reluctance to respond.

56.9% (143 people) wanted repurchase doctors and 36.3% (91 people) were thinking about 93.2%. Significant numerical results indicate the continuity of the patient's visit to the dentist if the periodontal disease treatment adjuvant is introduced. In addition, the sale of treatment supplements is considered to be a single treatment, and it is expected to be a way to increase the trust of patients and doctors.

[Evaluation of Treatment Effectiveness]

In order to evaluate the therapeutic efficacy of the composition for treating and preventing periodontal disease according to an embodiment of the present invention, the quantitative value (concentration) of the causative agent of periodontal disease-causing bacteria before and after ingestion of the composition for treating and preventing periodontal disease was analyzed. . That is, the periodontal disease adjuvant composition using the natural extract prepared as in Example was prepared as a drug recipe W. (Recipe W) in the tablet form to be taken by the subjects. The treatment efficacy evaluation was commissioned by Hallym University and was given a clinical approval number, such as “IRB Approval Number 14-1-58, Hallym University Institutional Review Board.”

1. Selection of research subjects and investigation

A total of 30 subjects consisted of adults aged 25 to 75 years. The average age of the subjects was 44.73 years. Patients diagnosed with mild chronic simple periodontitis were selected. Patients who had not received treatment for periodontal disease within the last 6 months and who voluntarily agreed and signed the subject's consent It was made.

In addition, those who are taking commercially available gum supplements, pregnant or breastfeeding patients, uncontrolled medical disorders, systemic diseases that are difficult to treat, chronic painful or chronic consuming or inflammatory diseases, Those who participated in other clinical trials within three months, or other ethical or clinical trial results, were excluded if the investigator determined that participation in the trial was inappropriate.

2. Evaluation of Treatment Effectiveness

Before and after 2 weeks and 3 weeks after the drug was taken, the sample was collected and sealed in a preservative solution and container of the cytoperio analysis system (cytogen co. Ltd). Co. Ltd,) was sent to a laboratory for genetic testing, extracted DNA from the sample and attached a DNA probe (fluorescent marker) and analyzed the type and quantitative value (concentration) of the causative agent causing periodontal disease by real-time PCR. The causative agents of investigation were aggregatibacter actinomycetemcomitans (Aa), porphyromonas gingivalis (Pg), tannerella forsythia (Tf), treponema denticola (Td), prevotella intermedia (Pi), fusobacterrium nucleatum (Fn), parvimonas micra (Pm), campylob camp A total of 11 bacteria were identified, including eikenella corrodens (Ec), streptococcus mutans (Sm), and streptococcus sorbinus (Ss).

That is, the reduction of bacteria was confirmed by quantitative analysis of each bacterium before and after taking the drug and after 3 weeks of taking the drug. A total of 30 subjects were included in a single group. Thirty subjects measured the reduction of periodontal disease-causing bacteria after two weeks of administration, and thirteen subjects measured the reduction of periodontal disease-causing bacteria after three weeks of administration. . Since the test results did not follow the normal distribution in all strains as an objective evaluation method before and after treatment, the statistical significance was evaluated by the Wilcoxon signed rank test, a nonparametric size comparison method, and evaluated under the conditions shown in Table 1 below. Was determined at P <0.05.

Case Processing Summary
	cases
	Valid		Missing		Total
	N	Percent	N	Percent	N	Percent
Diff.Tf	30	100.0%	0	0.0%	30	100.0%

3. Evaluation of Treatment Effectiveness

The treatment efficacy evaluation conducted by the above methods and conditions analyzed the quantitative value (concentration) of the causative agent of periodontal disease-causing bacteria before, after 2 weeks and after 3 weeks of taking the drug, and the results are shown in Table 2 below.

		Aa	Pg	Tf	Td
More than 2 weeks	Before taking	0	21382332	10298430	47041163
	After taking	0	16504553	5480590	5817856
More than 3 weeks	Before taking	0	20383273	8129878	42030895
	After taking	0	15337081	4731004	5202282
		Pi	Fn	Pm	Cr
More than 2 weeks	Before taking	64815254	160339949	283459510	4336081
	After taking	37146351	145480408	164115238	3481178
More than 3 weeks	Before taking	58720532	94083099	230300803	3961375
	After taking	29870291	98704686	154636079	3411109
		Ec	Sm	Ss	Total bacteria
More than 2 weeks	Before taking	282089497	17428088	8887872	16057105726
	After taking	261607670	10542716	3768729	13247838500
More than 3 weeks	Before taking	247341264	9748103	2949669	7764196812
	After taking	228009316	5192111	1479841	5005580520

Table 2 shows the 11 bacterial levels causing periodontal disease, aggregatibacter actinomycetemcomitans (Aa) was not detected in all subjects. In addition, the levels of the remaining 10 bacteria except for Aa were all decreased, and the levels of 11 bacteria, which cause periodontal disease, were higher when taking drugs for 3 weeks or more than when taking drugs for 2 weeks or more. The decrease was found to be greater. The decrease rate of the 11 bacterial bacteria causing the periodontal disease caused by taking the drug for two weeks or more is shown in the graphs of FIGS. 2 and 3, and the cause of the periodontal disease caused by taking the drug for three weeks or more 11 The rate of reduction of bacterial levels of the species is shown in the graphs of FIGS. 4 and 5.

In addition, among 11 species causing the periodontal disease, it was confirmed that the reduction rate of the bacteria such as treponema denticola (Td) and parvimonas micra (Pm) was greater, and the reduction rate of the Td and Pm bacteria was shown in the graphs of FIGS. Indicated.

Considering the results of quantitative value (concentration) of periodontal disease-causing bacteria shown in Table 2 above, the number of bacteria was increased and decreased or the number of bacteria was not changed, and the statistical significance of the normality and P value of the treatment efficacy evaluation test. The summarized results are shown in Tables 3 and 4 below. Table 3 below summarizes the statistical significance according to the normality and P value of the treatment efficacy evaluation test before and after 2 weeks of taking the drug, and Table 4 below shows the treatment corresponding to before and after 3 weeks of taking the drug. It is the result that summarized the statistical significance by the normality and P-value of the efficacy evaluation test.

Referring to Tables 3 and 4 below, periodontal subjects ingested for 3 weeks or more than those subjects ingested for 2 weeks or more of the composition for treating and preventing periodontal disease using a natural extract prepared according to an embodiment of the present invention It was confirmed that the reduction rate of quantitative value (concentration) of disease-causing bacteria appeared more.

Unit (Number of Research Subjects)		Aa	Pg	Tf	Td	Pi	Fn
More than 2 weeks	Fungus increase	-	5	15	One	7	14
	Bacteria reduction	-	6	12	9	12	16
	Fungus equal	30	19	3	20	11	-
	Normality	X	O	O	O	O	O
	p value	-	0.657	0.665	0.009	0.295	0.734
	valence	-	X	X	O	X	X
Unit (Number of Research Subjects)		Pm	Cr	Ec	Sm	Ss	Total bacteria
More than 2 weeks	Fungus increase	9	4	9	9	16	13
	Bacteria reduction	19	8	7	9	12	17
	Fungus equal	2	18	14	12	2	-
	Normality	O	O	O	O	O	O
	p value	0.04	0.388	0.796	0.948	0.633	0.299
	valence	O	X	X	X	X	X

Unit (Number of Research Subjects)		Aa	Pg	Tf	Td	Pi	Fn
More than 3 weeks	Fungus increase	-	3	6	One	2	7
	Bacteria reduction	-	3	5	3	6	6
	Fungus equal	13	7	2	9	5	0
	Normality	X	O	O	O	O	O
	p value	-	0.463	0.814	0144	0.123	0.382
	valence	-	X	X	X	X	X
Unit (Number of Research Subjects)		Pm	Cr	Ec	Sm	Ss	Total bacteria
More than 3 weeks	Fungus increase	4	2	3	5	5	4
	Bacteria reduction	9	4	5	2	6	9
	Fungus equal	0	7	5	6	2	-
	Normality	O	O	O	O	O	O
	p value	0.173	0.600	0.401	0.310	0.929	0.033
	valence	X	X	X	X	X	O

Therefore, in order to evaluate the therapeutic efficacy of the composition for treating and preventing periodontal disease according to one embodiment of the present invention, the quantitative value (concentration) of the causative agent of periodontal disease-causing bacteria before and after ingestion of the composition for treating and preventing periodontal disease According to the analysis results, no aggregatibacter actinomycetemcomitans (Aa) was detected in all the subjects, and among the 10 causes of periodontal disease, more than half of the patients showed a decrease in the number of bacteria. Td), prevotella intermedia (Pi), fusobacterrium nucleatum (Fn), parvimonas micra (Pm), and campylobacter rectus (Cr). Pg is anaerobic bacilli, closely related to acute or chronic periodontitis, and high frequency in periodontal sac of adult periodontitis patients. It was investigated. Td is a helix bacteria and is detected in most parts of periodontal pockets and is known to be associated with acute progressive periodontitis and necrosis or ulcerative periodontitis. Pi is a black pigment producing bacterium that causes hormone-related periodontitis and promotes complex infections. Fn is an anaerobic Gram-negative bacterium and is known to be detected with high frequency with helix bacteria in almost all areas where acute necrotic ulcerative gingivitis occurs. Pm is an anaerobic Gram-positive cocci and has been reported to be associated with recurrent or refractory periodontal disease and implant failure. Cr is an anaerobic Gram-negative bacillus and is known to cause periodontal pocket formation and secondary bacterial infections. In addition, it was confirmed that the amount of microbial reduction after taking Td and Pm also showed statistical significance.

In conclusion, in this study, we could overcome 11 limitations of existing periodontal disease-causing bacteria known as periodontal disease-causing bacteria by PCR method, and overcome several limitations that existed in culture methods. Could be obtained. As a result of comparing the microbial quantitative analysis by collecting gargle solution two weeks after the first visit of each group and taking food, Recipe W. is considered to be effective as a mechanical treatment in chronic periodontitis patients. Therefore, the meaning of reducing the bacterial quantitative value of the pathogenic microorganisms contained in the gingival fissure after taking the recipe W prepared with the composition for treating and preventing periodontal disease according to one embodiment of the present invention is to reduce the causative agent of periodontitis. This is expected to have a positive effect on the healing of advanced periodontitis.

Although the present invention has been described in detail with reference to the embodiments and examples, the present invention is not limited to the above embodiments, and may be modified in various forms, and is commonly used in the art within the technical spirit of the present invention. It is evident that many variations are possible by those of skill in the art. In addition, various forms of substitution, modification, and alteration may be made by those skilled in the art without departing from the technical spirit of the present invention described in the claims, which are also within the scope of the present invention. something to do.

The present invention can be used in combination with dental treatment because it was prepared using a natural extract for the treatment and prevention of periodontal disease can be useful in the treatment of periodontal disease, and can be taken in the long term without depending on antibiotics Since it is a composition for treating and preventing periodontal disease using a natural extract, it can be usefully used for long-term periodontal disease treatment and prophylaxis.

Claims (4)

1. Calcium, Avocado Extract, Water Soluble Propolis Extract, Corn Extract, Cinnamon Extract, Thistle Extract, Vitamin C, Vitamin B1, Vitamin B2 and Vitamin B6 Compositions for the treatment and prevention of periodontal disease using natural extracts .
2. The method according to claim 1,

   Composition for treating and preventing periodontal disease using the natural extract,

   Calcium 35-60%, Avocado Extract 0.5-7%, Water Soluble Propolis Extract 5-25%, Corn Extract 2-15%, Cinnamon Extract 0.5-7%, Thistle Extract 2-15%, Vitamin C 1 to 10% by weight, vitamin B1 0.01 to 0.3% by weight, vitamin B2 0.01 to 0.3% by weight and vitamin B6 0.01 to 0.3% by weight composition for treating and preventing periodontal disease using a natural extract, characterized in that .
3. The method according to claim 1 or 2,

   In the composition for the treatment and prevention of periodontal disease using the natural extract,

   Composition for the treatment and prevention of periodontal disease using natural extracts, characterized in that the addition of any one or more from the additive group consisting of silicon dioxide, magnesium stearate, crystalline cellulose and a color coating agent to form a tablet form.
4. The method according to claim 3,

   0.1 to 1 parts by weight of silicon dioxide, 0.1 to 1.5 parts by weight of magnesium stearate, 12 to 26 parts by weight of crystalline cellulose, and 1 to 2 parts by weight of colorant, based on 100 parts by weight of the composition for treating and preventing periodontal disease using the natural extract. Composition for the treatment and prevention of periodontal disease using natural extracts, characterized in that the addition of 5 parts by weight to prepare a dosage form.

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