KR101517095B1 - Periodontal diseases treatment and prevention composition using the natural extracts - Google Patents

Abstract

The present invention relates to a composition for treating and preventing periodontal diseases using natural extracts, and more specifically, to a composition for treating and preventing periodontal diseases using natural extracts, which is formed by containing calcium, an avocado extract, a water-soluble propolis extract, a corn extract, a cinnamon extract, a thistle extract, vitamin C, vitamin B1, vitamin B2 and vitamin B6. The present invention prepares a composition for treating and preventing periodontal diseases using natural extracts to be used in parallel with dental treatment, and thus the composition is helpful for treating periodontal diseases, and can be taken for a long period of time without depending on antibiotics. Additionally, the present invention includes all the ingredients excellent in three functions required for dental treatment, such as an osteoplastic function, an immunity strengthening function, and a periodontitis prevention and treatment function.

Description

TECHNICAL FIELD The present invention relates to a composition for treating and preventing periodontal disease using natural extracts,

The present invention relates to a composition for treating and preventing periodontal disease using natural extracts. More particularly, the present invention relates to a composition for treating and preventing periodontal disease using natural extracts, The present invention relates to a composition for treating and preventing periodontal disease using a natural extract having an advantage of being able to take long-term use without dependence on antibiotics.

In modern society, the number of people with erroneous eating habits and the number of patients suffering from periodontal disease due to reduced immunity or nutritional and metabolic disorders due to aging are on the rise. Such a health functional food and a therapeutic agent having an effect of preventing or treating periodontal disease so that the periodontal disease patient can take it are currently being developed.

However, it is difficult to take a long-term treatment of periodontal disease because synthetic medicines made of synthetic materials and the use of antibiotics cause adverse effects such as abuse of antibiotics due to the use of synthetic medicines, and most of the periodontal medicines, There are many problems because the effect of treating the disease is not proven and it is put on the market. In order to solve such a problem, development of a therapeutic agent for periodontal disease and an agent for preventing periodontal disease using natural extracts, that is, plant extracts, has been progressing actively.

Korean Patent No. 10-0431170 discloses a composition for treating periodontal disease containing a plant extract, which comprises one or two selected from the group consisting of cinnamon, lucidum, horseshoe chinensis, gugija, bokbunja, The present invention provides a composition for treating periodontal disease, which comprises as an active ingredient, a plant extract having excellent inhibitory activity against periodontal disease-causing microorganisms and having excellent inhibitory activity of proteolytic enzymes secreted by periodontal disease-causing bacteria Which is excellent in the treatment of periodontal disease.

Korean Patent No. 10-1358507 also relates to a composition for oral anti-inflammatory, antibacterial and deodorant containing red ginseng and a natural plant-derived extract, which is characterized in that it contains an anti-inflammatory, antibacterial and deodorant effect A composition for anti-inflammation, antibacterial and deodorization, and a process for producing the same.

In addition, Korean Patent Laid-Open No. 10-2012-0117387 discloses an oral composition for preventing and alleviating gum disease, which is useful for gum and tooth health such as kelp, shiitake mushroom, green tea extract, tocopherol, and propolis, Which is used as a raw material to provide gum, tooth health and oral cleansing effect.

The present invention relates to a method for the treatment and prevention of periodontal disease and to a method for the prevention and treatment of periodontal disease, A composition for treating and preventing periodontal disease, which is effective for chronic periodontal disease and implant periosteum, which maximizes the effect of periodontal disease treatment, including a functional element that enhances bone formation and enhances immunity, Has not been developed.

Korean Registered Patent No. 10-0431170 (registered, April 30, 2004) Korean Registered Patent No. 10-1358507 (Registered, Apr. 21, 2014) Korean Patent Laid-Open No. 10-2012-0117387 (published, 24 October 2012).

The present invention has been made to solve the above-mentioned problems,

It is an object of one embodiment of the present invention to provide a composition for treating and preventing periodontal disease, which can be used in combination with a dental treatment by using a natural extract, and thus can be useful for the treatment of periodontal disease, And to provide a composition for treating and preventing periodontal disease using natural extracts that can be taken. More specifically, calcium, avocado extract, corn extract, vitamin C and water-soluble propolis extract, corn extract, cinnamon extract and vitamin C, which are useful for periodontal disease, and thistle extract , Vitamin B 1, vitamin B 2 and vitamin B 6 are included so that the composition for treatment and prevention of periodontal disease includes all components effective for the three functions required for the dental treatment. Therefore, chronic periodontal diseases and implants It is an object of the present invention to provide a composition for treating and preventing periodontal disease using natural extracts effective for prevention and treatment of peripheral inflammation.

In order to achieve the above object, according to one embodiment of the present invention,

One embodiment of the present invention is a method for producing a natural extract comprising calcium, an avocado extract, a water-soluble propolis extract, a corn extract, a cinnamon extract, a thistle extract, vitamin C, vitamin B1, vitamin B2 and vitamin B6 A composition for treating and preventing periodontal disease is provided.

In the present invention, the composition for treating and preventing periodontal disease using the natural extract may comprise 35 to 60 wt% of calcium, 0.5 to 7 wt% of avocado extract, 5 to 25 wt% of water-soluble propolis extract, 2 to 15 wt% of corn extract, 0.5 to 7 wt% of cinnamon extract, 2 to 15 wt% of thistle extract, 1 to 10 wt% of vitamin C, 0.01 to 0.3 wt% of vitamin B1, 0.01 to 0.3 wt% of vitamin B2 and 0.01 to 0.3 wt% of vitamin B6 And the like.

In addition, in the present invention, at least one of additives from the group consisting of silicon dioxide, magnesium stearate, crystalline cellulose and color coating agent is further added to the composition for treating periodontal disease using the natural extract to prepare a tablet formulation . ≪ / RTI >

In addition, in the present invention, 0.1 part by weight to 1 part by weight of silicon dioxide, 0.1 to 1.5 parts by weight of magnesium stearate, 12 to 26 parts by weight of crystalline cellulose are added to 100 parts by weight of the composition for treating and preventing periodontal disease using the natural extract. And 1 to 5 parts by weight of a color coating agent are further added to prepare a tablet formulation.

The composition for the treatment and prevention of periodontal disease using the natural extract according to one embodiment of the present invention is a composition for treating and preventing periodontal disease which can be ingested while being combined with dental treatment, Therefore, it can be helpful to prevent periodontal disease and to treat periodontal disease.

In addition, since it is a composition for treating and preventing periodontal disease, which is composed of natural extracts that are not antibiotics or chemical raw materials, it is possible to minimize side effects that may occur in the human body due to abuse of antibiotics, And it is possible to increase the therapeutic effect of periodontal disease.

The composition for the treatment and prevention of periodontal disease using the natural extract according to an embodiment of the present invention includes all of three components necessary for dental care, namely bone formation function, immunity enhancement function and periodontal inflammation prevention and treatment function Therefore, it is effective in preventing and treating periodontal disease.

The present invention relates to a method for the treatment and prevention of periodontal disease, comprising the steps of: (1) preparing a composition for treating and preventing periodontal disease, which comprises a water-soluble propolis extract, a corn extract, a cinnamon extract, and vitamin C, Since the composition for treating and preventing periodontal disease is prepared by adding the ingredient for promoting bone formation and the ingredient for enhancing immunity, it is possible to provide a composition for preventing and treating periodontal disease, Is advantageous.

In other words, it strengthens the alveolar bone and periodontal ligament around the teeth and acts to maintain the dental implants and components such as water-soluble propolis extract, corn extract, cinnamon extract and vitamin C, which are useful for prevention and treatment of periodontal inflammation It contains ingredients such as calcium, avocado extract, corn extract and vitamin C that help prevent and cure inflammation around the implant. It helps the body's overall immune system, By preparing components for the treatment and prevention of periodontal disease using natural extracts such that thistle extract, vitamin B1, vitamin B2 and vitamin B6, which directly contribute to inflammation, are added to all of the above 10 components, Helps to prevent and treat periodontal disease, Since the reinforcement has the advantage of being exceptionally prevention and treatment of existing periodontal disease treatment and prevention of periodontal disease the composition than for.

FIG. 1 is a questionnaire survey conducted on an adjuvant composition for the treatment and prevention of periodontal disease according to an embodiment of the present invention. FIG.
Figure 2 shows the distribution of subjects in the survey.
FIG. 3 shows the distribution of personnel according to the symptoms of the subjects of the survey.
Figure 4 shows the general characteristics of the questionnaire.
FIG. 5 shows whether or not the subject of the questionnaire is taking the drug.
FIG. 6 shows the effect of Recipe W felt by the questionee.
FIG. 7 shows the effect of the questionnaire on the other products in comparison with the other products.
8 shows the improvement of the periodontal condition felt by the subject of questionnaire.
FIG. 9 shows the improvement of the symptom of the subject to be surveyed.
FIG. 10 shows a method of repurchasing an adjuvant composition for treating periodontal disease according to an embodiment of the present invention.
FIG. 11 shows the results of the evaluation of the therapeutic efficacy of the Recipe W prepared with the adjuvant composition for periodontal disease treatment and prevention according to one embodiment of the present invention. This graph shows the rate of decrease of the bacterial count.
FIG. 12 shows the result of the evaluation of the therapeutic efficacy of the Recipe W prepared with the adjuvant composition for the treatment and prevention of periodontal disease according to one embodiment of the present invention. As shown in FIG. 12, 11 kinds of Recipe W This graph shows the rate of decrease of the bacterial count.
13 is a graph showing the reduction rates of treponema denticola (Td) and parvimonas micra (Pm) in the results of the treatment efficacy evaluation test.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art. Therefore, the embodiments of the present invention can be modified into various other forms, and the scope of the present invention is not limited to the following embodiments.

Throughout the description of the present invention, when a section is referred to as " including " an element, it does not exclude other elements, but includes other elements as well, unless specifically stated otherwise.

The composition for the treatment and prevention of periodontal disease using the natural extract according to one embodiment of the present invention (hereinafter, also referred to as a composition for the treatment and prevention of periodontal disease) comprises calcium, avocado extract, water-soluble propolis extract, corn extract, Extract, thistle extract, vitamin C, vitamin B1, vitamin B2 and vitamin B6.

Three essential functions for the prevention and treatment of periodontal disease in dentistry are bone formation, prevention and treatment of periodontal inflammation, and immunity enhancement.

Ingredients such as calcium, avocado extract, corn extract, and vitamin C are excellent bone formation because they help to maintain the implant prosthesis strong and prevent inflammation around the implant.

In addition, ingredients such as water-soluble propolis extract, corn extract, cinnamon extract and vitamin C are effective in preventing or treating periodontal inflammation because it strengthens the alveolar bone and periodontal ligaments around the teeth.

In addition, ingredients such as thistle extract, vitamin B1, vitamin B2 and vitamin B6 help the body's overall immunity, and are especially helpful for skin inflammation, Is beneficial for malnutrition and metabolic disorders and is useful for prevention and treatment of periodontal disease, so it has excellent immunity enhancement effect.

Accordingly, the composition for treating and preventing periodontal disease using the natural extract according to one embodiment of the present invention is a composition for treating and preventing periodontal disease, which comprises calcium, avocado, water-soluble propolis, corn, cinnamon, Since it is constituted by including vitamin B6, it is necessary to help bone formation, to prevent and treat periodontal inflammation, and to enhance immunity. Therefore, By preparing a composition for the treatment and prevention of periodontal disease, there is an advantage that the composition for treating and preventing periodontal disease is superior to the conventional composition for the prevention and treatment of periodontal disease.

The composition for treating and preventing periodontal disease using natural extracts according to one embodiment of the present invention comprises 35 to 60% by weight of calcium, 0.5 to 7% by weight of avocado extract, 5 to 25% by weight of water-soluble propolis extract, Vitamin B, 0.01 to 0.3% by weight of vitamin B1, 0.01 to 0.3% by weight of vitamin B6, 0.01 to 0.3% by weight of vitamin B6, %, Based on the total weight of the composition.

1. Calcium

Calcium is a constituent of the skeleton and teeth. It contains 99% of the calcium content in the body and is involved in maintaining normal function of muscles and nerves. It regulates the contraction and relaxation of skeletal and cardiac muscle cells and is a nutrient essential for many enzymes involved in blood coagulation. Bone is composed of osteoblast and osteoclast cells. In osteoblast cells, collagen is secreted to form bone support structure, and osteoblast cells are matured into osteocytes, resulting in bone calcification . Therefore, the higher the calcium intake, the higher the calcium concentration in the blood, and calcium can be used to form the bone. On the other hand, osteoclasts are stimulated by parathyroid hormone and are largely activated when they eat calcium-deficient meals, releasing calcium from the bone into the blood by activated osteoclasts. The lowering of blood calcium concentration promotes osteoclast activation. Since bones are formed and degraded by bone turnover (bone remodeling) of osteoblasts and osteoclasts, calcium intake is important for bone formation and maintenance.

Calcium promotes osteogenesis and plays an important role in metabolism and metabolic regulation hormones. Among them, calcium helps bone formation, and when used as a composition for the treatment and prevention of periodontal disease, activates osteoblasts and inhibits osteoclasts This can increase the probability of success of implants and bone transplantation, accelerate bone formation around implants, accelerate initial fixation, and increase implant success rate. In other words, calcium is needed to form bones and teeth, plays an important role in maintaining nerve and muscle function, and is necessary for normal blood coagulation. Therefore, calcium can be used to improve recovery and function after bone grafting, It is a necessary ingredient.

According to one embodiment of the present invention, calcium is preferably contained in an amount of 35 to 60% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract. It is known that the content is set according to the functional functional food labeling standard, and the content of calcium is 220 to 660 mg per day according to the functional food functional labeling standard.

According to one embodiment of the present invention, when calcium is contained in an amount of less than 35% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease, the total weight of the composition for treating and preventing periodontal disease and the weight of components other than calcium When considered together, the calcium content in the composition for treating and preventing periodontal disease is less than the minimum daily ingestion according to the health functional food functional labeling standard, and the calcium effect may not be expressed properly. When calcium is contained in an amount of more than 60% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease, considering the total weight of the composition for treating and preventing periodontal disease and the weight of components other than calcium, The content of calcium contained in the composition for treating and preventing periodontal disease is excessively greater than the maximum daily ingestion amount according to the labeling standard, which may cause harmful effects due to excessive supply of calcium.

Excessive intake of calcium may cause nephrolithiasis, Milk-alkaline syndrome, which is caused by ingestion of milk-absorbing alkaline gels for a long period of time, and has symptoms of renal failure with or without hypercalcemia and alkalosis. ), Moderate alkalosis, abnormal serum phosphatase levels, severe renal failure with hyperglycemia and calcification, calcium-mineral interactions, and the like.

Therefore, calcium is preferably contained in an amount of 35 to 60% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract. In general, calcium trisodium phosphate can be used to produce a composition for treating and preventing periodontal disease using a natural extract according to an embodiment of the present invention, but other kinds of calcium may be used.

2. Avocado Extract

Avocado (Persea americana Mill) is a fruit known to originate in Mexico and Central America, and is widely consumed worldwide. Avocado contains 20 essential nutrients and a variety of phytochemicals. In other words, the lipid content to be edible is about 25%, it contains a lot of monounsaturated fatty acids and carotenoids, vitamin B, vitamin C, vitamin E, terpenoids, sitosterol, persenone A Persenone B, Phenol, protein, fiber, manganese, phosphorus, iron, potassium and folic acid. 100 g of avocado contains 18.7 g of fat, 720 mg of potassium, 0.21 mg of vitamin B2, 61.90 mg of vitamin C, 71.80 mg of vitamin B6, 1.34 mg of vitamin E and 5.30 mg of dietary fiber.

Antioxidant activity, inhibition of Acetyl CoA carboxylase, and antimicrobial activity of avocado have been reported. Especially, total polyphenol and flavonoid contents in the methanol extracts of avocado pulp, seed and skin were measured. The total polyphenol contents were 13.89 ㎍ / ㎎ of avocado extract, 137.2 ㎍ / ㎎ of seed extract, 223.45 ㎍ / ㎎, indicating that the polyphenol contents of seeds and shells were 10 to 20 times higher than those of avocado flesh.

The avocado extract has an excellent antioxidant activity because of its excellent polyphenol content, and the activity of the glycoprotein (ALP) of the cell membrane increases with the increase of the avocado concentration, thereby increasing the osteoblast. In addition, the avocado extract has an effect of reducing the activity of TRAP enzyme (cytotoxic labeling enzyme capable of measuring the degree of bone destruction in rats) without cytotoxicity, and inhibits osteoclast differentiation. In the case of osteoblasts, (ALP) activity, whereas osteoclasts have been reported to be cytotoxic. Thus, it has been proved that avocado extract proliferates osteoblasts and inhibits osteoclasts. Avocado extract also has the effect of helping to synthesize collagen and proteoglycan. Therefore, it plays an important role in strengthening the bone by inhibiting osteoclasts that induce the proliferation of osteoblasts that secrete collagen to form a supporting structure of the bone and induce calcium to release into the blood in the bone.

Since avocado extract helps bone formation by osteoblast proliferation and osteoclast inhibition, it has excellent effects on recovery and function improvement after bone grafting, implant and periodontal surgery when used as a composition for the treatment and prevention of periodontal disease .

According to one embodiment of the present invention, the avocado extract is preferably contained in an amount of 0.5 to 7% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract.

According to one embodiment of the present invention, when the avocado extract is contained in an amount of less than 0.5% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease, the total weight of the composition for treating periodontal disease and the amount of the component other than the avocado extract Considering the weight together, the content of the avocado extract contained in the composition for treating and preventing periodontal disease is too small, so that the effect of the osteoblast proliferation and osteoclast inhibitory action of the avocado extract is not expressed properly, have. When the total weight of the composition for treating and preventing periodontal disease and the weight of components other than the avocado extract are considered together when the total weight of the composition for treating and preventing periodontal disease is more than 7% by weight of the avocado extract, The content of the avocado extract contained in the composition for treating and preventing is excessively excessive and the vitamin E contained in the avocado extract is overdosed in excess of the daily intake standard so as to prevent the absorption of vitamin K in the body , Reduce platelet aggregation, cause postoperative hemorrhage, and cause harmful symptoms such as gastrointestinal disorders, muscle weakness, headache and chronic fatigue.

Accordingly, it is preferable that the avocado extract is contained in an amount of 0.5 to 7% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract. The avocado extract may be prepared by a conventional method known to those skilled in the art. Preferably, avocado is washed, dried and pulverized, and then extracted with an organic solvent such as methanol or ethanol. have. In the present invention, avocado extract (Avocado Fruit 10: 1 Extract (Persea Gratissima)), which was extracted by washing and drying avocado and pulverizing and then adding ethanol, was purchased and used.

3. Water-soluble propolis extract

Propolis is a material used for honey bee self defense or honey bee house collection, collected from sap of tree sap and blossoms of fruit juice and flower by bees. It is mainly used for resin, beeswax, essential oil, pollen, various organic and mineral substances And it has been reported to be effective not only in antioxidant activity but also in antibacterial, antifungal, antiviral, antitumor, human immunity reinforcement, wound healing and the like. Various organic and mineral substances in propolis contain more than 20 kinds of various flavonoids, and these flavonoids are reported to exhibit strong antibacterial activity. The flavonoids of propolis are all aglycons that distinguish them from flavonoids of other plants, and these compounds are involved in the antibacterial action of propolis. In addition, since propolis does not have antimicrobial action against intestinal bacteria, it is a safe natural antimicrobial substance because it does not adversely affect intestinal bacteria useful for human body, that is, bifidobacteria or lactobacillus, even when administered orally.

Propolis has been reported to have excellent antioxidant activity and antimicrobial activity in the oral cavity. The water-soluble flavonoid mixture is effective for the treatment of inflammation of the mucous membranes, and has an excellent function of treating mucosal inflammation such as clinically inhibiting gingival bleeding in periodontal disease patients. In addition, antibacterial and antioxidant activities of flavonoids reduce cavities And flavonoids are effective in decreasing acid production in human oral saliva and reducing caries activity. Accordingly, propolis is actively being developed as a component of oral health-related foods or products such as oral cleansers, periodontal disease treatments or adjuvants, toothpastes and the like.

In particular, patients suffering from periodontal disease are suffering from inflammation occurring in various periodontal diseases, especially after implantation, and suffer from many problems. Patients take antibiotics for the treatment of inflammation, and long-term antibiotic use has the potential to cause resistance. Therefore, propolis has been attracting attention as a therapeutic agent for inflammation using a natural extract which can not be tolerated and can be taken for a long term.

Propolis extract, which is used as a health functional food, is prepared by removing wax from propolis, which is made by blending its secretion with resin taken from a plant, and extracting it with water or alcohol, or extracting it with carbon dioxide, will be. Total flavonoids should contain at least 1%, para (ρ) -cumaric acid and cinnamic acid should be identified. In addition, diethyleneglycol should not be detected.

Generally, in order to use it as a material for health functional foods, raw materials of propolis are extracted with an organic solvent. In rare cases, side effects such as allergy may occur due to organic solvents. In addition, since it has been reported that propolis may cause irritation or ulceration in the mucous membrane to cause hypersensitivity reactions such as asthma, the composition for treating and preventing periodontal disease of the present invention is preferably used for preventing or treating inflammation or ulcer of the mucous membrane, Propolis extract was used.

According to one embodiment of the present invention, the water-soluble propolis extract is preferably contained in an amount of 5 to 25% by weight based on the total weight of the composition for treating and preventing periodontal disease using natural extracts. The content of water-soluble propolis extract was determined by taking into account the amount of intake in the human body test in which the function was confirmed.

16 to 17 mg as the total flavonoid is most preferred for daily intake. The propolis extract used as a health functional food should contain at least 1% of total flavonoids, so that the water-soluble propolis extract is preferred to have a daily intake of 160 to 200 mg.

According to one embodiment of the present invention, when the water-soluble propolis extract is contained in an amount of less than 5% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease, the total weight of the composition for treating and preventing periodontal disease, The content of the water-soluble propolis extract contained in the composition for the treatment and prevention of periodontal disease is lower than the lowest daily intake level determined by taking into consideration the amount of the water-soluble propolis extract in terms of the amount of the water-soluble propolis extract Therefore, there is a possibility that the antioxidative effect of the water-soluble propolis extract and the antimicrobial effect in the oral cavity may not be properly manifested. When the water-soluble propolis extract is contained in an amount of more than 25% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease, the total weight of the composition for treating and preventing periodontal disease and the weight of components other than the water- , The content of the water-soluble propolis extract contained in the composition for treating and preventing periodontal disease is excessively higher than the highest daily intake according to the health functional food functional labeling standard, and the amount of the propolis extract Allergic reaction and mucosal inflammation or ulceration.

Accordingly, it is preferable that the water-soluble propolis extract is contained in an amount of 5 to 25% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract. The water-soluble propolis extract may be prepared by a conventional method known in the art. Preferably, the non-processed propolis raw material is heated to remove beewax, The extracted extract may be used. In the present invention, a water-soluble propolis extract (Water Soluble Bee Propolis Ext. 70%) prepared by removing non-wax by adding heat to unpolished propolis raw materials and adding a spirit and then extracting it was used .

4. Corn extract

Corn extract (Zea Mays L.) is widely used as a component of health functional foods and products for the purpose of preventing periodontal diseases or periodontal diseases. This is due to the fact that Zea Mays L. (hereinafter also referred to as "ZML"), an undetectable quantitative extract derived from corn, has been found to be effective in the improvement of puerperium and periodontitis in 1958, It has been proved experimentally that there is a preventive and regenerating effect on alveolar bone resorption and periodontal ligament destruction when administered, and since the clinical results show that the effect of ZML on tooth dangling is slightly decreased and the depth of the periodontal ligament is also decreased. In addition, ZML showed that the inflammatory degradation of bone was stopped at about 80% after topical treatment, and the ZML showed that the bone nourishment process It was confirmed that it was effective in alleviating periodontal disease.

The corn extract contains phytosterol and squalene hydrocarbons (Squlaene), which are mainly composed of beta-sitosterol, stigmasterol and ergosterol, carotene and carotene, which are fat-soluble vitamins, and tocopherol Tocopherol) and the like. In particular, the composition for treating and preventing periodontal disease according to one embodiment of the present invention uses beta-sitosterol containing 10% or more of beta-sitosterol.

Beta-sitosterol is the most widely distributed plant sterol, a precursor of vitamin D, a type of provitamin D that stimulates calcium absorption, promotes alveolar bone formation, regenerates periodontal ligament fibers, Normal and anti-inflammatory function is excellent. Beta-sitosterol activates osteoblast-like cells to stimulate osteocyte production, promote regeneration of the alveolar bone, and prevent the alveolar bone from absorbing and strengthening the gum. It also has the ability to regenerate destroyed periodontal ligament fibers and prevent abnormal tooth movement. In addition, the composition for treating and preventing periodontal disease according to one embodiment of the present invention is excellent in the ability to restore injured epithelial cells and to strengthen the gums and to resist the bacterial infection, It is important to use a corn extract that contains at least 10%.

According to one embodiment of the present invention, the corn extract is preferably contained in an amount of 2 to 15% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract. It is a content determined on the basis of the content of corn extract which is registered as a periodontal disease in the general medicine, and the daily intake is preferably 80 to 120 mg.

According to one embodiment of the present invention, when the corn extract is contained in an amount of less than 2% by weight based on the total weight of the composition for treating and preventing periodontal disease, the total weight of the composition for treating and preventing periodontal disease, The content of the corn extract contained in the composition for treating and preventing periodontal disease is too small to activate the osteoblast cell of the corn extract and promote calcium absorption as a precursor of vitamin D, There is a fear that the effects such as promotion of regeneration and regeneration of periodontal ligament fibers are not properly manifested and added. When the total weight of the composition for treating and preventing periodontal disease and the weight of components other than corn extract are considered together when the corn extract is contained in an amount exceeding 15% by weight based on the total weight of the composition for treating and preventing periodontal disease, The content of the corn extract contained in the composition for treatment and prevention is excessively excessive, the production cost may increase and the productivity may be deteriorated, and it is preferable because it may affect the ingredient content of the composition for treating and preventing periodontal disease not.

Accordingly, it is preferable that the corn extract is contained in an amount of 2 to 15% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract. The corn extract may be prepared by a conventional method known in the art. Preferably, corn and corn hairs are washed together, dried and then pulverized, and ethanol or water is added to the pulverized pulverized product And extracts prepared by extracting corn can be used. In general, corn-deficient quantitative extracts, which are widely used in the manufacture of periodontal medicine, can be used. In the present invention, the corn extract (GMO Free Corn Silk with 10% Beta Sitosterol (Zea Mays)) prepared by washing and drying the corn, followed by pulverizing, adding ethanol to the pulverized product, .

5. Cinnamon extract

Cinnamon has been used as a treatment for headache, fever, neurogenic palpitations, pain and analgesia in the herb, which is a dried epidermis of evergreen tropical cinnamon trees belonging to the genus Lauraceae. The main ingredient of cinnamon is essential oil called cinnamon oil. Cinnamic aldehyde, Camphene cineol, Linalool, ERugenol, carbohydrate, fat, phosphorus, iron, Vitamin A, vitamin B1 and vitamin B2, it is widely used as a fragrance ingredient for beverages, chewing gum, toothpaste and cosmetics, and as a bad breath removing agent, and has been reported to have excellent antioxidative and anti-ulcerogenic effects have. In addition, stimulating the gastric mucosa to stimulate the secretion of the stomach, suppressing the spastic pain and promoting the movement of the gastrointestinal tract, the discharge of gas and good absorption is also effective. Cinnamon also stimulates the nerves to promote blood circulation, warm the body and suppress the abnormal fermentation of the intestines is also a preservative effect. Shinnam acid aldehyde in cinnamon also has a function of sustained peripheral vasodilating action (blood pressure lowering) and inhibiting platelet aggregation, and also has effects such as sweating and dusting action. It is widely used for cold, analgesia, and scent, and cinnamon extract has excellent antimicrobial activity that inhibits decay of food.

Cinnamon extracts can be used as natural antibiotics and bad breath remover because they have a unique flavor and excellent antioxidant and anti-inflammatory action. In particular, it is an important component in a composition for the treatment and prevention of periodontal disease, because it acts as a natural antibiotic together with water-soluble propolis, which is a component of the composition for treating periodontal disease according to the present invention.

According to one embodiment of the present invention, the cinnamon extract is preferably contained in an amount of 0.5 to 7% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract.

According to one embodiment of the present invention, when the content of the cinnamon extract is less than 0.5% by weight based on the total weight of the composition for treating and preventing periodontal disease, the total weight of the composition for treating and preventing periodontal disease, The content of the cinnamon extract contained in the composition for the treatment and prevention of periodontal disease is so small that the antioxidant and antiinflammatory effects of the cinnamon extract are not properly manifested and the natural antibiotic may not be properly performed . When the total weight of the composition for treating and preventing periodontal disease and the weight of components other than the cinnamon extract are considered together when the cinnamon extract is contained in an amount of more than 7% by weight based on the total weight of the composition for treating and preventing periodontal disease, The content of the cinnamon extract contained in the composition for treatment and prevention is excessively excessive and can be fatal to people with fever or heat, and the productivity may be lowered due to increased production cost, It is undesirable because it may affect the component content of the composition.

Accordingly, it is preferable that the cinnamon extract is contained in an amount of 0.5 to 7% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract. The cinnamon extract may be prepared by a conventional method known in the art. Preferably, the cinnamon extract may be pulverized and extracted with water, followed by filtration, concentration and purification. have. In the present invention, the extraction process of adding water to the cinnamon followed by extraction at 75 to 85 ° C for 2 hours is repeated three times, followed by filtration, the filtrate is concentrated at a temperature of 75 to 85 ° C and purified Cinnamon Bark 2: 1 Extract (Cinnamomum Zeylanicum), which was prepared by drying, was purchased and used.

6. Thistle extract

Thistle (Milk Thistle) is a plant of Asteraceae, the scientific name is Silybum marianum (L.) Gaertn, the tinnitus is Carduus silybum, and fruit and seed are used for food. Traditionally, herbologists have used thistle (milk-seed) seeds to protect the liver, such as chronic epilepsy. In major European countries, the silymarin component of thistle (milky seed) protects hepatocytes from active oxygen, Because it is known to be used as a liver remedy or dietary supplement.

Thistle (Milk Seed) extract is prepared by pulverizing thistle (Milk Thistle), extracting it with alcohol, filtering, concentrating and purifying the product. Silymarin (320 to 660 mg / g. < / RTI > Silymarin is composed of four isomers of Silybin (A, B) and Silychristin, Silydianin, Isosilybin (A, B).

Thistle (Milk Seeds) contains a component called Silymarin (Silymarin) is said to have excellent efficacy to improve liver function. Since the antioxidant activity is 10 times that of vitamin E, it enhances the secretion of glutathione, facilitates hepatic detoxification, and is highly effective in regenerating the liver tissue, which helps to regain liver function. Silymarin, which is extracted from the seeds of thistle, inhibits the proliferation of cancer cells. The results of the study show that it is effective in the prevention and treatment of inflammation and liver damage, which are side effects of cancer patients receiving cancer treatment. It turned out to be excellent.

In other words, the silymarin ingredient of thistle (Milk Seeds) is used as a therapeutic agent for liver and dietary supplements because it has excellent antioxidative properties such as protecting hepatocytes from free radicals and is useful for liver health. Because of the direct nature of the dentist's skin and immunity, liver function restoration is very important for the treatment of periodontal disease. Therefore, the silymarin component of thistle (milky seed) is effective for the recovery of liver function, so it is a necessary nutrient for recovery and function improvement after bone grafting, implant and periodontal surgery. Therefore, composition for treatment and prevention of periodontal disease Is an essential raw material ingredient for

According to one embodiment of the present invention, it is preferable that the thistle extract is contained in an amount of 5 to 15% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract. This is the content set according to the functional food functional labeling standard.

In Korea, thistle seeds and berries are used as food ingredients, and thistle extracts are also used as general medicines. Thistle extract is also used as dietary supplements and medicines in other countries. It is listed as food in the Prehensine Database and Dietary Supplements in the United States Pharmacopoeia (USP-NP). In the WHO monograph, the daily intake of silymarin was recommended to be 200-400 mg. However, considering the safety as a health functional food, If the content is set according to the standard, it is preferable that the daily intake is 80-240 mg based on silymarin.

According to one embodiment of the present invention, when the thistle extract is contained in an amount of less than 5% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease, the total weight of the composition for treating periodontal disease and the amount of components other than thistle extract The content of thistle extract contained in the composition for the treatment and prevention of periodontal disease was so small that the content of the thistle extract was lower than that of the lowest daily intake determined in consideration of the content set in accordance with the functional functional food labeling standard, There is a fear that the effect of restoring the function is not properly manifested and it is not meaningful to add it. In addition, when the thistle extract is contained in an amount exceeding 15% by weight with respect to the total weight of the composition for treating periodontal disease, the total weight of the composition for treating and preventing periodontal disease and the weight of components other than thistle extract are considered, The content of thistle extract contained in the composition for the treatment and prevention of periodontal disease is excessively higher than the highest daily intake amount determined in consideration of the content set in accordance with the food functional labeling standard, and harmful symptoms such as diarrhea, headache and arthralgia There is a concern.

Accordingly, it is preferable that the thistle extract is contained in an amount of 5 to 15% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract. The thistle extract may be prepared by a conventional method known in the art. Preferably, the thistle is pulverized and extracted with a syrup, followed by filtration, concentration and purification. . In the present invention, milk thistle extract (Milk Thistle 80% Silymarin (Silybum marianum)) prepared by pulverizing thistle and then extracting with addition of alcohol and concentrating and stagnating the extracted extract was purchased and used.

7. Vitamin C

Vitamin C is a six-carbon lactone. Plants and most animals can synthesize vitamin C in the body, but humans, primates, guinea pigs, big bats, some species of birds and fish can synthesize Therefore, it must be consumed in the form of food. Vitamin C includes both reduced form of ascorbic acid and oxidized form of dehydroascorbic acid, both of which are interchangeable. In fact, there are two forms of food that we eat.

Vitamin C has a function to form connective tissues. It is used as a coenzyme of various enzymatic reactions in vivo and is involved in the synthesis of collagen, which is a main protein constituting connective tissues such as skin, skeleton, blood vessels and cartilage. When the amino acids Proline and Lysine are hydrolyzed to form Hydroxyproline and Hydroxylysine, Vitamin C plays a role in reducing the iron content of Hydroxylase. And the collagen tissue is maintained.

The electron donating function of vitamin C plays a role in inducing antioxidant activity to remove free radicals such as Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS). Especially, tissues that are susceptible to damage from free radicals such as eyes, neutrophils, and semen are known to have a relatively high content of vitamin C. In addition, since it prevents the initiation of lipid peroxidation such as low density lipoprotein (LDL) I am involved. In addition, the vitamin C radical has a relatively long half-life of 5 to 10 seconds compared with other antioxidants, and thus it is recognized as a stable antioxidant.

Vitamin C is known to have excellent iron absorption effects such as promoting the absorption of non-hemoglobin-conjugated vegetable iron by converting iron to reduced form in the small intestine. In other words, vitamin C is a necessary component for the formation of connective tissues and functions, and it helps to absorb iron and protects cells from harmful oxygen. Therefore, recovery and function improvement after bone grafting, implant and periodontal surgery It is a necessary ingredient for.

According to one embodiment of the present invention, vitamin C is preferably contained in an amount of 1 to 10% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract. This is the amount set by the recommended daily intake of adult men and women.

The average vitamin C requirement of adult men and women is 75 mg / day, and the recommended intake is 100 mg / day. The recommended intake is based on the amount that maintains the white blood cell's vitamin C concentration to a maximum while keeping the amount of urine excreted to a minimum.

When the total weight of the composition for the treatment and prevention of periodontal disease and the weight of the components other than vitamin C are considered together when the vitamin C content is less than 1% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease, The amount of vitamin C contained in the composition for treating and preventing periodontal disease is less than the average amount required for the periodontal disease, and it is not meaningful to add vitamin C, and the effect of vitamin C may not be properly manifested. When the total weight of the composition for treating and preventing periodontal disease and the weight of components other than vitamin C are considered together when vitamin C is contained in an amount exceeding 10% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease, The amount of vitamin C contained in the composition for treating and preventing periodontal disease is excessively greater than the recommended amount of vitamin C, which may cause problems due to excessive supply of vitamin C.

It is known that supplements containing supplemental vitamin C supplements are mainly gastrointestinal symptoms such as nausea, vomiting, abdominal bloating, abdominal pain and diarrhea. Excessive iron absorption and vitamin B12 levels are reported to be exacerbated by fisheries excretion, renal stone, uric acid excretion, and excessive iron absorption. In pregnant women, excessive vitamin C intake during pregnancy leads to vitamin C dependence in newborns. In animal tests and in vitro tests, vitamin C is over-prooxidized to cause harmful effects It is reported to be possible.

Therefore, vitamin C is preferably contained in an amount of 1 to 10% by weight based on the total weight of the composition for treating and preventing periodontal disease using natural extracts.

8. Vitamin B1

Vitamin B1 (Thiamin) is linked by a methylene bridge with a hexagonal ring of pyridine containing nitrogen at the center and a pentagonal ring of thiazole containing sulfur.

Pigs, sunflower seeds, beer yeast, beans, potatoes, wheat germ, whole grains and fortified grains, peas, asparagus, meat guts, peanuts and other seeds, mushrooms and watermelon are good sources of vitamin B1. As such, many foods containing vitamin B1 are generally low in content, so it is desirable to take a variety of foods.

Thiamin pyrophosphate (TPP), which is a coenzyme form of vitamin B1, contains two phosphate groups, such as Pyruvate, α-Ketoglutarate and Branched-Chain Keto acids. It serves to catalyze the decarboxylation of α-keto acids. It also catalyzes the keto transfection reaction of hexose phosphates and pentose phosphates. Transketolase, which is required for the keto-transfer reaction, is an enzyme that requires TPP and produces pentose from the hexose glucose in the pentose phosphate pathway. Vitamin B1 is therefore required for the metabolism of carbohydrates and branched amino acids (leucine, isoleucine, valine). That is, since vitamin B1 is involved in energy metabolism, the amount of vitamin B1 required is highly correlated with energy consumption.

Since vitamin B1 is required for carbohydrate and energy metabolism, when used as a composition for the treatment and prevention of periodontal diseases, it is possible to solve the disadvantage that patients with periodontal disease are susceptible to metabolism of carbohydrates which must be chewed and digested, Thereby performing an effective function for periodontal regeneration in chronic periodontal disease patients.

According to one embodiment of the present invention, the vitamin B1 is preferably contained in an amount of 0.01 to 0.3% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract. This is the amount set by the recommended daily intake of adult men and women.

The average intake of vitamin B1 in Korea is 1.0 mg / day for males and 0.9 mg / day for females. The recommended intake is 120 mg / day for male and 1.2 mg / day for female. The indicator used in setting the recommended intake is transketolase activity of red blood cells. The activity of this enzyme is measured by comparing the activity with and without the addition of the coenzyme of vitamin B1 after destroying blood cells.

When the total weight of the composition for the treatment and prevention of periodontal disease and the weight of the components other than vitamin B1 are considered together when the composition contains less than 0.01% by weight of vitamin B1 relative to the total weight of the composition for the treatment and prevention of periodontal disease, The content of vitamin B1 contained in the composition for treating and preventing periodontal disease is less than the average amount of vitamin B1 contained in the composition for preventing and treating periodontal disease. Therefore, it is not meaningful to add vitamin B1 and the effect of vitamin B1 may not be properly manifested. When the total weight of the composition for the treatment and prevention of periodontal disease and the weight of the components other than vitamin B1 are considered together when vitamin B1 is contained in an amount exceeding 0.3% by weight based on the total weight of the composition for the treatment and prevention of periodontal disease, The amount of vitamin B1 contained in the composition for treating and preventing periodontal disease is excessively greater than the recommended amount of vitamin B1, which may cause problems due to overproduction.

Naturally, the human toxicity of vitamin B1 in foods is very low, but toxicities have been reported when high concentrations of vitamin B1 have been ingested for therapeutic purposes. High-vitamin B1 causes symptoms of central nervous system disorders such as headache, nausea, anxiety, insomnia, tachycardia, and lack of aura. It stops when vitamin B1 is stopped or reduced in volume.

Therefore, it is preferable that the vitamin B1 is contained in an amount of 0.01 to 0.3% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract.

9. Vitamin B2

Vitamin B2 (Riboflavin, riboflavin) is a molecule with three hexagonal rings. It is usually found in the form of Flavin adenine dinucleotide (FAD) and Flavin mononucleotide (FMN) in the biosystem. Vitamin B2 in free form is very small. Vitamin B2 is relatively stable in acid, heat, and oxidation, but is weak in alkali or light. Animal foods and dairy products such as liver, meat, poultry, and fish are good sources of vitamin B2. In addition, beans, green vegetables, grains and turbulence are also sources of vitamin B2.

Vitamin B2 has excellent caloric metabolism. The coenzyme of vitamin B2 is involved in various enzymatic reactions, especially in caloric metabolism. Various types of vitamin B2 enzymes are involved in the metabolism pathway of carbohydrates, fats and amino acids, including dehydrogenation, hydroxylation, oxidative decarboxylation, dioxygenation, Reduction reaction.

When vitamin B2 deficiency occurs, deficiency symptoms such as erosion of the mouth, nose and throat appearing in the mouth, seborrhea, nose and mouth, facial and scrotum around the mouth, seborrheic dermatitis around the mouth, eye redness and photophobia, zoster cataract and anemia appear . Therefore, when vitamin B2 is deficient, it causes various symptoms to the oral cavity, and proper intake of vitamin B2 is effective for periodontal disease and chronic periodontal disease because it has excellent energy generation and metabolism. In particular, the composition for treating and preventing periodontal disease of the present invention has a high content of plant extracts, i.e., lipid-soluble components, which may lead to inadequate metabolism. The addition of vitamin B2 can facilitate metabolism, It is a necessary ingredient for enhancing the effect of the composition for use.

According to one embodiment of the present invention, the vitamin B2 is preferably contained in an amount of 0.01 to 0.3% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract. This is the amount set by the recommended daily intake of adult men and women.

The average amount of vitamin B2 required can be determined based on the activity of glutathione reductase (RBC) and the excretion of vitamin B2 in the urine. The average amount of vitamin B2 in Korean adults is 1.3 mg / day for male, 1.0 mg / day for female . The recommended intake is 120% of the average intake, 1.5 mg / day for men and 1.2 mg / day for women.

When the total weight of the composition for the treatment and prevention of periodontal disease and the weight of the components other than vitamin B2 are considered together when the composition contains less than 0.01% by weight of vitamin B2 relative to the total weight of the composition for treating periodontal disease and prevention, The amount of vitamin B2 contained in the composition for the treatment and prevention of periodontal disease may be lower than the average amount required for the periodontal disease treatment and may not effectively affect the caloric metabolism (metabolic pathway of carbohydrate, fat, and amino acid) There is no meaning or the effect of vitamin B2 may not be expressed properly. When the total weight of the composition for the treatment and prevention of periodontal disease and the weight of the components other than vitamin B2 are considered together when the total amount of vitamin B2 is more than 0.3% by weight based on the total weight of the composition for treating periodontal disease, The amount of vitamin B2 contained in the composition for the treatment and prevention of periodontal disease is excessively higher than the recommended amount of vitamin B2 in the composition for the prevention and treatment of periodontal disease and the ability of the human gastrointestinal tract to absorb vitamin B2 is limited. , The excretion of vitamin B2 is suddenly increased and yellow urine may be excreted.

Therefore, it is preferable that the vitamin B2 is contained in an amount of 0.01 to 0.3% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract.

10. Vitamin B6

Vitamin B6 is present in foods as pyridoxine (PN), pyridoxal (PL), pyridoxamine (PM), or each phosphorylated form (PLP, PNP, PMP). In animal foods, PLP, PMP and protein are present in a combined form. In the case of plant foods, PN, PNP and sugar are present in a combined form. The coenzyme forms of vitamin B6 are PLP and PMP, and PLP is especially a form of biohazard. The free form of vitamin B6 is a substance that is relatively unstable and susceptible to denaturation, although its degree varies depending on the acidity.

The sources of vitamin B6 include fish, pork, chicken, turbulence, and animal foods such as intestines (liver, kidney), as well as brown rice, soybeans, and oats. Dairy products have a relatively low content of vitamin B6.

Vitamin B6 is a necessary component for protein and amino acid utilization. PLP is a coenzyme of enzymes involved in the metabolism of more than 100 kinds of amino acids such as aminotransferase, decarboxylase and stereoselective enzyme (Recemase). For example, PLP is required for reactions that synthesize non-essential amino acids. If PLP is not available, all amino acids will be essential amino acids and must be supplied through meals. In addition, PLP is a stereoisomeric enzyme that acts on the conversion of D-amino acids to L-amino acids. When proteins are synthesized in the human body, only L-type amino acids are used, so the action of PLP is important. In addition, vitamin B6 acts as a coenzyme of homocysteine catabolism and phosphorylase reaction, and thus is a necessary ingredient for maintaining the homocysteine level of blood to be normal.

Clinical deficiency symptoms of vitamin B6 include interstitial coma, dermatitis, stomatitis, keratitis, nephritis, depression, heart abnormality, and abnormalities in the use of anemia, fatty liver, and body energy in animals have been reported. Thus, vitamin B6 is a necessary ingredient for the prevention and treatment of oral diseases such as stomatitis, bulimia, and sulphitis. In particular, the liver function is restored by the action of thistle (milk seed) extract, which is a component of the composition for the treatment and prevention of periodontal disease of the present invention, and the vitamin complex of vitamin B1, vitamin B2 and vitamin B6 is added to milksh seed ), The metabolism is facilitated, thereby enhancing the immunity and excelling in the treatment and prevention of periodontal disease.

According to one embodiment of the present invention, it is preferable that vitamin B6 is contained in an amount of 0.01 to 0.3% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract. This is the content determined by the recommended daily intake of adult men and women.

The average intake of vitamin B6 in Korean adults is 1.3 mg / day for males and 1.2 mg / day for females. The recommended intake is 120 mg / day for males and 1.5 mg / day for males and 1.4 mg / day for females.

When the total weight of the composition for the treatment and prevention of periodontal disease and the weight of the components other than vitamin B6 are considered together when the composition contains less than 0.01% by weight of vitamin B6 relative to the total weight of the composition for the treatment and prevention of periodontal disease, The content of vitamin B6 contained in the composition for treating and preventing periodontal disease is lower than the average amount of vitamin B6 contained in the composition for preventing and treating periodontal disease. Therefore, there is a fear that the addition of vitamin B6 is not meaningful and the effect of vitamin B6 is not properly manifested. When the total weight of the composition for the treatment and prevention of periodontal disease and the weight of the components other than vitamin B6 are considered together when the total amount of vitamin B6 is more than 0.3% by weight based on the total weight of the composition for treating periodontal disease, The amount of vitamin B6 contained in the composition for treating and preventing periodontal disease is excessively greater than the recommended amount of vitamin B6.

Vitamin B6 supplements that are taken from foods have not been reported, but it has been reported that long-term use of medicines for the treatment of diseases such as premenstrual syndrome can lead to sensory neurosis or skin lesions. Taking high doses of vitamin B6 may cause nerve damage such as nerve damage, tingling of hands and feet, staggering gait, loss of sensation around the mouth, and loss of cooperative muscle function.

Accordingly, it is preferable that vitamin B6 is contained in an amount of 0.01 to 0.3% by weight based on the total weight of the composition for treating and preventing periodontal disease using the natural extract.

Accordingly, the composition for treating and preventing periodontal disease using the natural extract according to one embodiment of the present invention comprises 35 to 60% by weight of calcium, 0.5 to 7% by weight of avocado extract, 5 to 25% by weight of water-soluble propolis extract, 2 to 15% by weight of cinnamon extract, 0.5 to 7% by weight of cinnamon extract, 2 to 15% by weight of thistle extract, 1 to 10% by weight of vitamin C, 0.01 to 0.3% by weight of vitamin B1, 0.01 to 0.3% Most preferably 0.3% by weight.

According to one embodiment of the present invention, at least one of the additives selected from the group consisting of silicon dioxide, magnesium stearate, crystalline cellulose and color coating agent is further added to the composition for treating and preventing periodontal disease using the natural extract, .

This is a method for preparing a composition for the treatment and prevention of periodontal disease using the above-mentioned natural extract, which comprises adding the components of the additive group in order to make it easy to ingest, comprising adding silicon dioxide, magnesium stearate, crystalline cellulose, Is preferably composed of a component which does not interact with each component of the composition for treating and preventing periodontal disease using the natural extract and has no effect on the effective side.

According to one embodiment of the present invention, 0.1 to 1 part by weight of silicon dioxide, 0.1 to 1.5 parts by weight of magnesium stearate, and 0.1 to 1 part by weight of silicon dioxide are added to 100 parts by weight of the composition for treating and preventing periodontal disease using the natural extract, 12 to 26 parts by weight of cellulose, and 1 to 5 parts by weight of a color coating agent, to prepare tablets.

The content of the additive group consisting of silicon dioxide, magnesium stearate, crystalline cellulose and color coating agent is suitable for preparing a composition for treating and preventing periodontal disease using natural extracts in a tablet form, And the composition to be prevented from affecting the effectiveness of the composition for prevention. Thereby, 0.1 to 1 part by weight of silicon dioxide, 0.1 to 1.5 parts by weight of magnesium stearate, 12 to 26 parts by weight of crystalline cellulose and 1 to 5 parts by weight of a color coating agent are added to 100 parts by weight of a composition for treating and preventing periodontal disease using natural extracts To prepare a formulation in tablet form.

[ Example ]

A composition for treating and preventing periodontal disease using the natural extract according to an embodiment of the present invention is prepared.

5 mg of calcium, 35 mg of avocado extract, 175 mg of water-soluble propolis extract, 100 mg of corn extract, 35 mg of cinnamon extract, 100 mg of thistle extract, 66 mg of vitamin C, 1.2 mg of vitamin B1 and 1.5 mg of vitamin B6 , And then further added with 5 mg of silicon dioxide, 10 mg of magnesium stearate, 280 mg of crystalline cellulose and 42 mg of color coating agent as a form of tablets to treat and prevent periodontal disease using natural extracts A composition is prepared.

[Poll]

The scope of application of the composition for the treatment and prevention of periodontal disease according to an embodiment of the present invention, the effect that the patients feel and the use state of the patient to be purchased are grasped and presented, and the questionnaire . The periodontal disease adjuvant composition using the natural extract prepared as in the above Example was prepared as a purified drug Recipe W and taken to the subjects. The results of the questionnaire and the questionnaire are shown as Recipe W.

1. Questionnaire design

FIG. 1 is a questionnaire survey conducted in order to understand the effects and the usage status of purchasing patients on the composition for the treatment and prevention of periodontal disease according to an embodiment of the present invention.

The questionnaire items of this study were classified according to the patient 's symptoms, whether they answered the call answer or not, the degree of effectiveness after taking Recipe W after one month, the degree of effectiveness compared with other products, , The status of re-purchase for one month, and the intention to continue taking it. For the selected items, more than 10 dentists from Seoul National University, Hallym University, and Pusan National University evaluated the appropriateness of the selection and expression of questionnaires, and they were validated with a statistical expert.

2. Selection of research subjects and conducting surveys

The purpose of this study was to inform the patients about the effect of Recipe W and its scope of application.

The subjects were 356 patients who purchased Recipe W in the dentist for 30 days from April 9, 2014 to May 8, 2014. Recipe W The number of patients in the purchase of the patients is 251 women and 105 men. The ages are 20s, 2s, 30s 4s, 40s 35s, 50s 127s, 60s 116s, 70s 65s, 80s (Fig. 2).

There were a total of 356 patients with symptoms (248 perimetry, chronic periodontal disease, 80 patients, general periodontal disease, 18 implants, and 6 osteoporotic patients) (Figure 3).

In this study, we conducted a preliminary survey because basic data should be provided. The telephone survey was conducted using CMG Changwon (service agency) to obtain objectivity. The period for conducting the telephone survey is 5 days from June 9 to 13, 2014, from 11:00 am to 1:00 pm, 2:00 pm to 5:00 pm, the maximum number of researchers to participate in the time difference between the phone call three times or not received the response was not answered. A total of 251 respondents (251 respondents) answered the questionnaire.

3. Results

3-1. General characteristics of survey subjects

A total of 251 patients were recruited for Recipe W periodontal disease supplements in the Wu Dental clinic. Peripheral periosteum was the most common (182 cases, 70%), followed by chronic periodontal disease (59 cases, 23%), periodontal disease 4%), 7 patients planning implant (3%), and 1 patient substituting osteoporosis medicine (0%) (Fig.

3-2. Recipe  Duration and Effect of W

Patients who took more than one month steadily received 60.2% (151 patients), 22.7% (57 patients) for 2 months or more, and 17.1% (43 patients) for others.

Of the 251 respondents who participated in the survey, 61% (153) answered that they were effective, and 39% (98) answered that they were not effective. 35.1% (88 patients), 25.9% (65 patients), 14.3% (36 patients), and 12% (30 patients), respectively 9.6% (n = 24), and 3.2% (n = 8), respectively.

A total of 43 patients answered that they had taken other products for periodontal disease treatment, and their rankings are as follows. 49% (21 patients), 44% (19 patients), and 7% (3 patients), respectively.

3-3. Periodontal status  Improving

Sixty-two percent (237) of the respondents answered that the periodontal condition improved, and 14% (37) reported that the periodontal condition improved (Figure 8).

Of the 86% of the patients who reported improvement, the most common symptom was improvement in tooth movement and gum weakness (71 cases), pain and swelling improvement (61 cases), brushing improvement (59 cases), siren improvement (42 cases), and bad breath improvement 9).

4. Re-purchase  doctor

The actual re-purchase of 356 patients for 30 days from April 9, 2014 to May 8, 2014 was 22% (99 patients). Of the 251 telephone respondents, 56.9% (143 patients) wanted to repurchase, and 36.3% (91 patients) and 6.7% (17 patients) did not want to repurchase. Seven patients who did not want to buy again, seven patients who answered that propolis smell was strong, and three patients stopped taking after cure (Figure 10).

Therefore, 251 out of 356 respondents responded to the telephone survey. According to the symptoms of recipe W, 25% of the 251 respondents had periosteal implants 70% (182), chronic periodontal disease 23% (59), periodontal disease 4% (10), implant 3% As a substitute 0% (1 person), it is prevalent for patients with periplasmic infection, but it can be applied as a basis for introducing periodontal disease supplements when considering the number of patients. In addition, the results of the questionnaire can be considered as the final result when the proportion of the 356 subjects and the proportion of the 251 respondents are similar. The results of the recipe W follow-up showed that the patients who had taken the drug continuously had enough effect. Of the 173 patients who took more than one month, 22% (99 patients) Was 56.9% (143 patients), indicating the importance of taking the patient.

According to the effect of Recipe W, 61% (153) of the respondents answered that they had an effect, and the number of respondents who want to purchase again is 143. This is a fundamental part where steady dosing can judge the effect. Of the 39% (98 patients) who answered that they had no effect, there were 30 patients who did not take the medication properly, and 36 patients were taking preventive measures, 24 patients were not feeling well, and 8 patients were not It is an important part to guide the correct taking when. According to a survey of other products, 43 respondents (17%) took other products, suggesting that there may be a large number of patients dependent on drugs without periodontal care in dental hospitals and clinics. As a result, 93% of respondents (40 respondents) answered that they had some effect or a great effect. It is judged to be more important than other products distributed on the market. In the questionnaire survey on the improvement of periodontal status, 86% (237 cases) were improved and 14% (37 cases) were not improved. The conclusion to be drawn from this questionnaire suggests that there may be an improvement of 86% in about 2 months of taking Recipe W and that there is a significant improvement in taking it for longer periods. According to the symptom improvement questionnaire, 71 cases of improvement of tooth irregularity and gingival weakness were the most, 61 cases were improved pain and swelling, 59 cases were improved in brushing, 42 cases were improved, and 4 cases were improved in bad breath. , It is considered that the part about the bad breath is not felt by the patient or is reluctant to respond.

56.9% (143 people) wanted to purchase the telephone survey, and 36.3% (91 people) thought it was 93.2%. As a result of the significant figures, it means continuity of the patient to visit the dentist continuously if the periodontal disease treatment aid is introduced into the hospital. In addition, it is considered that the sale of the therapeutic additive is considered to be a single treatment and it will be a way to increase the trust of patients and physicians.

[Treatment efficacy evaluation]

In order to evaluate the therapeutic effectiveness of the composition for the treatment and prevention of periodontal disease according to an embodiment of the present invention, the quantitative values (concentration) of the causative bacteria causing periodontal disease were analyzed before and after ingestion of the composition for the treatment and prevention of periodontal disease . That is, the periodontal disease adjuvant composition using the natural extract prepared as in the above Example was prepared as a purified drug Recipe W, and was taken into the study subjects. The treatment efficacy evaluation was commissioned to Hallym University and received a clinical approval number as "IRB No. 14-1-58 of the Clinical Study Deliberation Committee of Kangdong Sacred Heart Hospital, Hallym University".

1. Selection of research subjects and conducting surveys

The study population consisted of 30 adults, aged between 25 and 75 years, and the average age of the subjects was 44.73 years. Patients who had been diagnosed with chronic mild periodontitis were selected. Patients who did not receive any treatment for periodontal disease within the last 6 months should understand the explanation of the clinical trial and voluntarily agree with the patient. Respectively.

In addition, patients who are taking conventional gum ancillary drugs, those who are pregnant or breastfeeding, those who have uncontrolled medical diseases, those who have difficulty in dental treatment, those who suffer chronic pain or suffer chronic wasting or inflammatory diseases Were excluded if they were found to have participated in other clinical trials within 3 months, or if they were found to be improperly participated in the clinical trial because they could affect other ethical or clinical trial results.

2. Treatment efficacy assessment method

Before and after 2 weeks of taking the drug and after 3 weeks of taking the drug, the gaggle extract solution was placed in a preservation solution and a container of the cytoperio analysis system (cytogen co. Ltd.), Sealed, and the cytoperio analysis system DNA was extracted from the specimens and attached to a DNA probe (fluorescence marker), and then analyzed by real-time PCR for the types and quantitative levels (concentrations) of causative bacteria causing periodontal disease. The causative organisms were Agrobacterium acinomycetemcomitans (Aa), porphyromonas gingivalis (Pg), tannerella forsythia (Tf), treponema denticola (Td), prevotella intermedia (Pi), fusobacterium nucleatum (Fn), parvimonas micra , Eikenella corrodens (Ec), streptococcus mutans (Sm) and streptococcus sorbinus (Ss) were detected.

That is, the decrease of bacteria was confirmed by quantitative analysis of each bacterium before and 2 weeks after the drug and after 3 weeks of taking the drug. A total of 30 subjects were included in the study. A total of 30 subjects who measured the reduction of periodontal disease causing bacteria 2 weeks after the administration, and 13 subjects who measured the decrease of periodontal disease causing bacteria 3 weeks after taking . The test results were evaluated objectively before and after treatment. The statistical significance was evaluated by the Wilcoxon signed rank test, which was a nonparametric comparison method, because all strains did not follow the normal distribution. Was determined at P < 0.05.

3. Results of treatment efficacy evaluation

The treatment efficacy evaluations conducted under the above-mentioned methods and conditions were carried out by analyzing the quantitative values (concentration) of causative bacteria causing periodontal disease before and after 2 weeks of taking and after 3 weeks of taking, and the results are shown in Table 2 below.

Table 2 shows 11 bacterial levels of periodontal disease causing bacteria, and aggregatibacter actinomycetemcomitans (Aa) was not detected in all subjects. In addition, all of the 10 other bacteria except Aa showed a decrease, and when the drug was taken for 3 weeks or longer, 11 bacterial counts The rate of decrease was found to be larger. The reduction rate of eleven species of bacteria that cause periodontal disease caused by taking the drug over two weeks is shown in the graph of FIG. 11. It is shown that 11 kinds of bacterium that cause periodontal disease when the drug is taken over 3 weeks The reduction rate of the numerical value is shown in the graph of FIG.

In addition, the rate of decrease of bacteria such as treponema denticola (Td) and parvimonas micra (Pm) was found to be larger among the 11 causative bacteria causing periodontal disease, and the reduction rate of Td and Pm was shown in the graph of FIG.

 The statistical significance of the regularity and the P value of the treatment efficacy evaluation test in consideration of the state in which the number of bacterial counts increases or decreases or the number of bacteria does not change is shown in Table 2 The results are summarized in Tables 3 and 4 below. The following Table 3 summarizes the statistical significance by the normality and the P value of the corresponding treatment efficacy evaluation test before and after 2 weeks of drug administration. Table 4 shows the results of the corresponding treatment after 3 weeks of drug administration And the statistical significance by the P value and the normality of the efficacy evaluation test.

In Table 3 and Table 4, it was found that the subjects who ingested more than 2 weeks of composition for treating and preventing periodontal disease using natural extracts prepared according to one embodiment of the present invention, (Concentration) reduction rate of disease causing microorganisms was found to be larger.

Therefore, in order to evaluate the therapeutic efficacy of the composition for treating and preventing periodontal disease according to one embodiment of the present invention, the quantitative values (concentration) of the causative bacteria causing periodontal disease before and after ingestion of the composition for treating and preventing periodontal disease In summary, aggregatibacter actinomycetemcomitans (Aa) was not detected in all subjects, and porphyromonas gingivalis (Pg), treponema denticola (Pg) Td), prevotella intermedia (Pi), fusobacterrium nucleatum (Fn), parvimonas micra (Pm) and campylobacter rectus (Cr). Among 6 causative bacteria with more than half of the total number of pathogens causing periodontal disease, Pg is an anaerobic bacterium, closely related with acute or chronic periodontitis, and present in the periodontal pouch frequently in adult periodontitis patients Respectively. Td is a sputum and is detected in most parts of the periodontal pouch, and is known to be related to acute progressive periodontitis and necrosis or ulcerative periodontitis. Pi is a black pigment producing bacterium and is known to cause hormone-related periodontitis and promote complex infection. Fn is an anaerobic gram-negative bacterium and is known to be detected at a high frequency in almost all sites of acute necrotizing ulcerative gingivitis, along with salivary germs. Pm is an anaerobic Gram-positive staphylococcus and has been reported to be associated with recurrent or intractable periodontal disease and implant failure. Cr is an anaerobic gram negative bacterium and is known to cause periodontal disease and secondary bacterial infections. In addition, it was confirmed that the amount of microbial decrease after double dosing of Td and Pm was also statistically significant.

In conclusion, in this treatment efficacy evaluation test method, 11 types of periodontal disease causing bacteria known as periodontal disease causative bacteria were analyzed by PCR method, and thus it was possible to overcome various limitations that existed in the conventional culture method, Results could be obtained. As a result of comparing the quantitative analysis of microorganisms collected from two weeks after ingestion of each army and food, it was concluded that recipe W is effective as an adjunctive treatment for mechanical surgery in patients with chronic periodontitis. Therefore, the reduction of the amount of pathogenic microorganisms contained in the gingival crevicular fluid after the administration of Recipe W prepared with the composition for treating periodontal disease according to one embodiment of the present invention means that the number of pathogenic microorganisms in the gingival cavity, Which is expected to have a positive effect on healing.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but many variations and modifications may be made without departing from the scope of the present invention. It is evident that many variations are possible by those skilled in the art. It will be apparent to those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. something to do.

Claims (4)

A composition for treating and preventing periodontal disease using natural extracts comprising calcium, avocado extract, water-soluble propolis extract, corn extract, cinnamon extract, thistle extract, vitamin C, vitamin B1, vitamin B2 and vitamin B6 .
The method according to claim 1,
The composition for treating and preventing periodontal disease using the natural extract,
A water extractable composition comprising 35 to 60 wt% of calcium, 0.5 to 7 wt% of an avocado extract, 5 to 25 wt% of a water soluble propolis extract, 2 to 15 wt% of a corn extract, 0.5 to 7 wt% of a cinnamon extract, 2 to 15 wt% The composition comprising 1 to 10% by weight of C, 0.01 to 0.3% by weight of vitamin B1, 0.01 to 0.3% by weight of vitamin B2 and 0.01 to 0.3% by weight of vitamin B6. .
The method according to claim 1 or 2,
In the composition for treating and preventing periodontal disease using the natural extract,
Wherein the composition is prepared in a form of tablets by adding at least one compound selected from the group consisting of calcium stearate, magnesium stearate, magnesium stearate, crystalline cellulose, and color coating agent.
The method of claim 3,
0.1 to 1 part by weight of silicon dioxide corresponding to the additive group, 0.1 to 1.5 parts by weight of magnesium stearate, 12 to 26 parts by weight of crystalline cellulose, and 1 to 5 parts by weight of a color coating agent 1 to 100 parts by weight of a composition for treating periodontal disease, And 5 parts by weight of the extract of the present invention. The composition for treating and preventing periodontal disease using the natural extract.

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