KR102141623B1 - Composition for prevention or treatment of dental disease comprising an extract of cinnamon - Google Patents

Abstract

The composition for preventing or treating inflammatory pain according to the present invention is to include cinnamon extract as an active ingredient.
The composition for preventing or treating inflammatory pain derived from the present invention can effectively relieve acute inflammatory pain and facial pain caused by oral diseases by relieving pain and activating saliva in the oral cavity.

Description

Composition for preventing or treating inflammatory pain comprising cinnamon extract as an active ingredient {COMPOSITION FOR PREVENTION OR TREATMENT OF DENTAL DISEASE COMPRISING AN EXTRACT OF CINNAMON}

The present invention relates to a composition for preventing or treating inflammatory pain comprising cinnamon extract as an active ingredient, effectively alleviating pain and secreting saliva in the oral cavity to effectively acute inflammatory pain and facial pain caused by oral diseases It relates to a composition that can be prevented or treated.

The inflammatory process is a series of complex biochemical and cellular events that are activated in response to the presence or damage of foreign substances, causing swelling and pain. The inflammatory process is a defense mechanism against conventional pathogenic factors, but can cause pain even in the absence of stimulation of toxic substances. Local inflammatory reactions release pro-inflammatory cytokinins and upregulate cyclooxygenase-2 to synthesize prostaglandins. This makes primary primary neurons sensitive, and activates neuron immunity by sensitizing nearby nerve cells. Therefore, a thermal or mechanical hypersensitivity occurs. When the inflammatory reaction as described above occurs, pain is involved. These pains are nociceptive pain, which is a kind of warning sensation caused by harmful stimuli that can damage or potentially damage tissue, and damaged tissue and surrounding tissue. Inflammatory inflammatory pain caused by inflammatory mediators such as IL-1β (interleukin 1) secreted by activation from the inflammatory receptor and neuropathy continuously appearing to non-toxic stimuli due to damage to the peripheral and central nerves It can be distinguished as pain (neuropathic pain).

In particular, the inflammatory pain includes cases that develop into neuropathic pain, such as trigeminal neuralgia and migraines, as well as acute facial pain due to damage to the teeth, alveolar bones, oral soft tissues and temporomandibular joints.

Inflammatory pain prevents further damage by limiting the movement of the pain area and disappears after a certain period of time, but it seems that it is important to control inflammatory pain because it may develop into neuropathic pain as it progresses in the direction of harm to ‹š.

The acute facial pain may occur during dental treatment, scaling, and other dental hygiene procedures, and is sometimes accompanied by sudden or regular pain without any cause.

The acute facial pain may be specifically divided into afferent blockage pain syndrome, trigeminal neuralgia, oral burning syndrome, and tension headache.

The centripetal blockage pain syndrome The centripetal blockage pain syndrome refers to a condition in which pain occurs continuously even when there is no cause of external pain causing sensitization when there is damage or inflammation of the sensory nerve. In this case, sudden pain may occur due to dental treatment, scaling, or other mild stimulation, and small pain may be felt largely. Most of the cases of burning, tingling, tingling, or dull pain in the adjacent area after treatment is removed from the dentist or after treatment with nerves or gums. In addition, the acute facial pain associated with the afferent pain syndrome is not treated well with general pain relievers, and many antidepressants and anticonvulsants are used. Recently, capsaicin cream or gabapentin may be used.

In addition, the trigeminal neuralgia causes shocking pain, such as sudden electricity in the face or mouth during daily life, and cannot move, and after that moment, the pain immediately disappears, but if you touch any part of the face or mouth, it becomes electricity again. It is characterized by recurring pain that seems to cause pain. Most of the patients appear in middle-aged or older, and the pain appears only on either side of the left or right side of the face or chin. It does not appear on both sides. These trigeminal neuralgia rarely have a therapeutic effect with general analgesics, and drugs such as carbamazepine, bacrofen, and amitriptyline should be used alone or in combination. The drug must be used for a long period of time, so you need to be careful about side effects and undergo regular blood tests. If medication is ineffective, surgery may be considered.

In addition, the burning sensation syndrome refers to feeling of pain in the mucous region of the tongue or mouth without any specific cause, and is mainly seen in postmenopausal women. Patients with burning sensation syndrome often complain of burning or tingling or soreness in the 2/3 area of the front of the tongue, the front of the palate, and the lips, but sometimes the symptoms such as dry mouth and taste disorders are complex. The exact cause of the burning sensation syndrome has not been identified, and it is estimated that only changes in hormones, psychological factors, and degenerative changes in the taste nerve are involved. In general, it is possible to relieve symptoms by administering amitriptyline and clonazepam or applying capsaicin cream, but it is not easy to cure. In the case of oral candidiasis, antifungal agents such as nistatin or ketoconazole should be used, and if dry mouth feels, pilocarpine or artificial saliva is prescribed.

Furthermore, the tension headache is the highest incidence among headaches, and the head appears to be tightened, heavy, or throbbing. It usually feels pain on both sides of the head and is worse in the afternoon than in the morning. The intensity of the pain is not constant, but it does not appear extreme. Stress or muscle tension are known to be the main causes of tension headaches, but other causes are also involved. Drug therapy is mainly used for the treatment of these tension headaches, but a better therapeutic effect can be obtained when both behavioral therapy and physical therapy are performed. Botox injections have recently been recommended as a treatment for tension headaches.

Therefore, the acute facial pain referred to in the present invention includes acute facial pain caused by afferent blockage pain syndrome, trigeminal neuralgia, oral burning syndrome, and tension headache, and includes sudden and temporary accompanying oral facial pain symptoms. .

In addition to these inflammatory pains, as the society ages, patients with degenerative arthritis and low back pain are increasing every year, but existing opioids such as morphine are limited in use due to drug action to the public, so the demand for pain relievers is much higher in the future. It is always expected. In addition, the development of painkillers for pain that does not respond to the existing painkillers is greatly demanded, and the general patient cannot use them conveniently due to the narcotic and serious side effects of the existing opioid drugs, which are known to have relatively high analgesic effects. Therefore, it is necessary to develop natural resources that have no side effects and have an analgesic suppression effect.

On the other hand, in oral health, saliva plays a very important role. Specifically, it is known to prevent dry mouth, protect the oral cavity from environmental stress, and inhibit oral pathogen adhesion to teeth by combining with oral lubrication and pathogens. Therefore, those who have less production of saliva, which is responsible for the health of the oral cavity, are relatively likely to be exposed to oral disease.

Saliva has two functions: food intake and oral environment maintenance. In the food intake function of saliva, digestive effects such as the formation of food lumps and digestive enzymes, solubilization of taste irritants, and secretion of gastrin It shows the action of maintaining the taste. In the oral environment maintenance function, it exhibits a self-cleaning effect of teeth or mucous membranes, a recalcification action of teeth, an antibacterial action, an immune action, a tissue repair promoting action by growth factors, and an anti-inflammatory action.

Saliva is composed of 99.5% moisture, 0.5% electrolyte, mucus, glycoprotein, enzymes, and antibacterial substances, and is secreted by the salivary glands into the oral cavity. . At this time, when the saliva secreted is less than 0.1 ml, it is called dry mouth (Xerostomia), and is the most common clinical symptom associated with salivary glands.

This reduction in saliva secretion, i.e., xerostomia, is accompanied by abnormalities and metabolic diseases of the salivary gland itself (e.g., Vasedow's disease, sugary mildew, etc.) or collagen disorders caused by radiation therapy or mumps. do. In addition, the function of the salivary glands is reduced by stress factors, side effects of various medicines, and aging.

Aquaporins (AQPs), which play an important role in salivation, are water channel proteins found in the cornea, kidneys, liver, and skin. Aquaporin has various subfamily from AQP0 to AQP12. Expression of AQP1 is concentrated in capillary endothelial cells and myoepithelial cells, whereas when AQP5 is deleted, it secretes hypertonic saliva with a markedly reduced capacity. AQP5 plays an important role in saliva production.

The decrease in saliva secretion, as a result of dry mouth, causes the tongue to turn red and sometimes cracks, so patients with decreased salivation complain of pain during feeding, and pain in chewing or swallowing. This can lead to facial pain and cause acute facial pain, causing discomfort in the mouth, or taste disorders and pronunciation disorders, causing denture instability, dental caries, gingivitis, mucosal ulcers in the mouth, bad breath and dry mouth. .

They mainly complain of abnormalities in chewing function and language function, and severe bad breath and cavities occur, and depending on the degree, they experience severe pain due to burning of the oral mucosa or ulceration of the oral mucosa. Although topical application of artificial saliva is used as treatment, its effectiveness is limited and limited. As saliva secretion accelerators, anerol trithion and sevimelin hydrochloride, known as muscarinic receptor agonists, are used, which have unstable effects and functional problems of digestive system side effects such as nausea, vomiting, loss of appetite, and abdominal discomfort. There are some drawbacks, such as. Therefore, there is an urgent need for the development of a composition for promoting salivation of natural ingredients without side effects different from compounds already known to have a salivation promoting effect.

(Patent Document 1) KR 10-2016-0045648 A1

An object of the present invention is to provide a composition for preventing or treating inflammatory pain comprising cinnamon extract as an active ingredient.

Another object of the present invention is to provide a composition for preventing or improving acute facial pain derived from a natural product capable of alleviating inflammatory pain.

Another object of the present invention is to provide a composition capable of effectively preventing or treating acute inflammatory pain and facial pain caused by oral disease by relieving pain and activating saliva in the oral cavity using cinnamon extract.

To achieve the above object, the composition for preventing or treating inflammatory pain according to an embodiment of the present invention is to include cinnamon (cinnamon) extract as an active ingredient.

The inflammatory pain may include acute inflammatory pain or acute facial pain caused by oral disease.

The oral disease may include those selected from the group consisting of dental caries, gingivitis, mucosal ulcers in the oral cavity, bad breath and dry mouth.

The cinnamon (cinnamon) extract includes extracting using an extraction solvent selected from the group consisting of water, C₁ to C₄ lower alcohols, and mixtures thereof.

The pharmaceutical product according to another embodiment of the present invention includes a composition for preventing or treating the inflammatory pain.

Functional food according to another embodiment of the present invention includes a composition for preventing or treating the inflammatory pain.

Hereinafter, the present invention will be described in more detail.

As used herein, the term'extract' has a meaning commonly used as a crude extract in the art as described above, but broadly also includes a fraction in which an extract is additionally fractionated. That is, the extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity), etc. The fraction obtained through the purification method is also included in the extract of the present invention.

The extract used in the present invention may be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

The term'comprising as an active ingredient' in the present specification means to include an amount sufficient to achieve the efficacy or activity of the extract according to the present invention. The present invention is a composition extracted from a natural plant material, and even when administered in excess, there is no side effect to the human body, so the upper limit of the amount of the extract contained in the composition of the present invention can be carried out by a person skilled in the art within an appropriate range.

The composition for preventing or treating inflammatory pain according to an embodiment of the present invention is to include cinnamon extract as an active ingredient.

Cinnamon (cinnamon) belongs to the camphoraceae (Lauraceae) and is native to southern China, Vietnam, Sri Lanka, Indochina and Korea (Jeju). As a main component of cinnamon, cinnamic aldehyde, eugenol, and the like are known, and since ancient times, it has been used in various ways, such as antiperspirants, antipyretics, painkillers, and food scents.

Physiologically active substances, such as cinnamic aldehyde and eugenol, have a unique flavor, and are therefore widely used as health supplements and medicines. Liquid products using cinnamon generally extract extracts of soluble substances in cinnamon Or it is prepared by adding a concentrated concentrate. The pharmacological efficacy of cinnamon is known for its anti-allergic effect, antibacterial effect, anti-ulcer effect, and hepatitis B virus suppression effect. In addition, it is known to have an antiseptic effect that promotes intestinal peristalsis and inhibits abnormal fermentation in the intestine, and research is being actively conducted as a natural antibacterial agent that inhibits microbial contamination and growth as a food additive, pharmaceutical, and industrial material. have. However, the results of pain-related research are very scarce, and the effectiveness in the development and prevention of inflammatory pain has been rarely proven.

When using the cinnamon extract, it is specialized in inflammatory pain, and can effectively suppress or alleviate the inflammatory pain.

Inflammatory pain referred to in the present invention includes postoperative, post-traumatic pain, arthritis (rheumatic) or osteoarthritis pain and pain associated with damage to the joints, muscles and tendons, as in the case of axial lower back pain. Pain that occurs when there is tissue damage or inflammation.

The inflammatory pain may include acute inflammatory pain or acute facial pain caused by oral disease.

More specifically, the inflammatory pain may be caused by trauma, surgery, infection and various oral diseases, and may include acute inflammatory pain or acute facial pain.

The acute facial pain is also referred to as acute oral facial pain or acute facial pain, and is generally temporary in the maxillofacial area such as the temporomandibular joint (jaw joint), jawbone (chin), face (face), mouth (tongue or cheek), head, neck and shoulders. Or, it means periodic acute pain, which can be distinguished from chronic pain caused by periodontal disease, etc., which can occur during dental treatment, scaling, or other hygiene procedures, and sometimes accompanied by sudden or regular pain without certain cause. Speak.

The oral disease may include dental caries, gingivitis, mucosal ulcers in the oral cavity, bad breath and dry mouth.

In the present invention, the oral disease is a concept that includes all irrespective of the pathology if it is a disease that occurs in the oral cavity, but for the purposes of the present invention, it may preferably mean oral disease due to oral immunity reduction.

Dental caries (cavity) refers to an oral disease in which the tooth decays and decays sugar, destroys the enamel or dentin of the tooth, and the teeth are pierced and turn black.

Periodontitis (periodontitis), also called periodontitis, is a disease that mainly occurs in the gums and bone tissue, and in the case of chronic infection, it means periodontal disease, in which cell tissue is destroyed to lose teeth.

Mucosal ulcers in the oral cavity are diseases caused by inflammation of the mucous membranes and the mouth of the mouth, mainly due to infections such as bacteria, viruses, and fungi, and stress, fatigue, hormonal changes, menstrual cycles, and sudden weight changes affect the onset. , Oral lubrication, it is also caused by a decrease in saliva secretion that can inhibit pathogen attachment in the oral cavity by combining with pathogens.

Halitosis (halitosis) is often referred to as bad breath, and due to acquired systemic diseases, saliva protein, food residues, etc., are decomposed by microorganisms, and the amino acids produced are mitigated by deoxidase or amino enzymes, causing odor. It is a disease.

Dry mouth (xerostomia) is a symptom in which saliva is not continuously secreted by any cause, causing the mouth to dry out. The amount of saliva may decrease due to systemic causes such as Sjogren's syndrome, anemia, diabetes, nutrient deficiency, and aging. , In addition to taking various drugs, it can also be caused by diseases of the nervous system.

Saliva is secreted by three large salivary glands of the parotid gland, submandibular gland and sublingual gland, and small salivary glands evenly distributed in the oral cavity. When the function of these tissues decreases, the secretion of saliva decreases, resulting in symptoms of dry mouth. When dry mouth appears, the protective effect in the mouth by salivary solutions, proteins, and mucins is markedly reduced, resulting in destruction of salivary tissue. When saliva secretion decreases and develops into chronic dry mouth syndrome, it changes pronunciation, makes food chewing activity and skipping difficult, causes burning sensation in the mouth and increases the risk of tooth decay and oral disease.

In actual clinical practice, pilocarpine or sevimelin is used to treat or alleviate dry mouth syndrome. In the case of pilocarpine administration at a dose of 15-30 mg per day, the pilocarpine effect was about 25%, and after dosing. It is known to be effective in about 50% of patients.

Dry mouth is generally caused by a decrease in saliva secretion. Saliva secretion is achieved by interaction of several complex factors, and it is known that aquaporins (AQPs) play an important role in saliva secretion.

Aquaporin 5 (Aquaporin 5, AQP5) is a characteristic water channel protein component that exists from AQP0 to AQP9 depending on the type of organs, such as the lens of the eye, kidney, stomach, tube, liver, and pancreas, and is a linear cell of the salivary gland. It is reported that AQP5 is abundantly expressed in, and, in particular, the production of aquaporin-5 (AQP5) is selectively increased by interferon-alpha.

On the other hand, saliva can also be promoted by agents containing electrolytes such as muscarinic agonist or adrenergic agonist. Amylase is a representative digestive enzyme found in saliva and serves to break down starch. Amylase is expressed in cell organs or progenitor cells and glandular cells of the salivary glands that are involved in protein secretion in the pancreatic progenitor cells of rats, and can be used as a preferred marker to judge the function of the salivary glands.

Currently, many studies have been conducted to treat salivation, but the underlying treatment is difficult and popular treatments are mainly performed. You can increase your fluid intake to keep your mouth moist, or use sugar-free chewing gum, take lemon-flavored drinks, use artificial saliva, and use a citric acid-added fern solution. In addition, although saliva preparations for sprays developed by dogs and electric stimulation methods have been used experimentally, research into the development of therapeutic agents for hyposalivation has been conducted because its effects are temporary and not limited. However, research on dental caries, gingivitis, oral mucosal ulcers, bad breath, and dry mouth associated with alleviating salivation is very lacking, especially in the prevention of acute inflammatory pain and acute facial pain caused by these oral diseases. The effectiveness has been little proven.

When the cinnamon (cinnamon) extract is used, it is specialized in oral diseases, and can effectively suppress or alleviate the inflammatory pain caused by it.

The cinnamon (cinnamon) extract includes extracting using an extraction solvent selected from the group consisting of water, C₁ to C₄ lower alcohols, and mixtures thereof.

Specifically, crushing the dried cinnamon; Leaching the pulverized material using an organic solvent; Drying the sample after leaching; Re-leaching the dried sample using an organic solvent; Drying the sample after leaching; Leaching with water; And comprising the step of leaching, it is possible to obtain a cinnamon extract.

The method may further include fractionating the natural extract extracted using the organic solvent using an organic solvent.

The method for preparing the extract may be a conventional extraction method in the art, such as ultrasonic extraction, leaching, and reflux extraction. Specifically, the natural product from which foreign substances have been removed by washing and drying may be an extract extracted with water, an alcohol having 1 to 6 carbons or a mixed solvent thereof, or an extract extracted by sequentially applying the solvents to a sample.

The reflux extraction method is based on 100 to 100 g of water, 1 to 6 carbon alcohols, 10 to 30 g of pulverized natural products, 1 to 3 hours reflux time, and 50 to 100% carbon 1 to 6 alcohols or water. More specifically, on the basis of 100 Ml of alcohol having 1 to 6 carbons or 100 mL of water, 10 to 20 g of natural products, 1 to 2 hours of reflux and 70 to 90% of alcohol or water having 1 to 4 carbons.

The leaching method proceeds for 15 to 30° C. for 24 to 72 hours, and water or alcohol having 1 to 6 carbon atoms of 50 to 100% is used as an extraction solvent. More specifically, it proceeds for 20 to 25°C and 30 to 54 hours, and the extraction solvent is by water or an alcohol having 1 to 6 carbon atoms of 70 to 80%.

The ultrasonic extraction method proceeds the reaction for 30 to 50°C, 0.5 to 2.5 hours, and the extraction solvent is by water or alcohol having 50 to 100% carbon number 1 to 6. Specifically, it is extracted at 40 to 50°C for 1 to 2.5 hours, and is extracted with water or an alcohol having 1 to 6 carbon atoms of 70 to 80% as an extraction solvent.

The extraction solvent may be used 2 to 50 times based on the weight of the sample, more specifically 2 to 20 times. For extraction, the sample can be left for 1 to 72 hours for leaching in the extraction solvent, and more specifically for 24 to 48 hours.

After extraction, the extract may be fractionated by sequentially applying a new fraction solvent. The fractionation solvent used for fractionation is at least one selected from the group consisting of water, hexane, butanol, ethylacetic acid, ethyl acetate, methylene chloride and mixtures thereof, preferably ethyl acetate or methylene chloride.

After obtaining the extract or fraction, a method such as concentration or lyophilization may be additionally used.

The composition for the prevention or treatment of inflammatory pain of the present invention can increase the effect by using a complex extract in which a natural extract is additionally mixed with a cinnamon extract.

Preferably, the natural extract may further include an extract selected from the group consisting of Gavin extract, Eumjigye extract, Sambaekcho extract and mixtures thereof.

The Gaebija (Cephalotaxus koreana Nakai) is an evergreen tree growing in a valley or in a forest, reaching a height of 3 m. The leaves are linear, arranged in two rows, the ends are sharply sharp, the front is green, and the vein is clear. Flowers bloom in April with Igahwa, and male flowers are flat, and two female flowers run in one place. Fruits are round, ripened in red in August-September of next year, seeds are oval, brown.

The Eumjigye is a perennial herb that grows in sunny forests or grasses with sceptridium ternatum (Thunb.) Lyon. The thick fleshy roots spread all over the place. The leaf is divided into 2, and it is composed of a nutritive lobe and a true lobe, and the lobe has long petiole, split into 3, and has serrate on the edge. The spore lobe is longer than the broad lobe and the upper part is split long, and the spore sac hangs on the branch.

The three hundred seconds (Saururus chinensis (Lour.) Baill.) is a perennial plant that grows in wetlands and is 50 to 100 cm high and has a white root. The leaves are coarse, pointed at the end, the bottom is heart-shaped, and the edges are flat. Flowers are benign, bloom white in June-August, and inflorescences cope with leaves. Fruits are round and consist of 3 to 5 threads, with 1 seed in each thread.

The composition for preventing or treating inflammatory pain is used in addition to a cinnamon extract, further comprising a natural extract selected from the group consisting of Gaeja biloba extract, Eumji cigarette extract, Sambaekcho extract and mixtures thereof, when used as a complex extract, the It may have a better effect on preventing or treating inflammatory pain.

Preferably, the composition of the present invention may contain 40 to 60 parts by weight of Gavinja extract, 40 to 60 parts by weight of Eumjigye extract, and 40 to 60 parts by weight of Sambaekcho extract with respect to 100 parts by weight of cinnamon extract. When used within the above range, the effect of preventing or treating inflammatory pain may be increased by a complex action between components.

The pharmaceutical product according to another embodiment of the present invention includes a composition for preventing or treating inflammatory pain.

The preferred dosage of the extract of the present invention depends on the patient's condition and body weight, the degree of disease, the drug form, the route and duration of administration, but can be appropriately selected by those skilled in the art. However, for the desired effect, the extract is preferably administered at 0.01 mg/kg to 10 g/kg per day, preferably at 1 mg/kg to 1 g/kg. The administration may be administered once a day, or may be divided into several times. Therefore, the above dosage does not limit the scope of the present invention in any way.

Functional food according to another embodiment of the present invention includes a composition for preventing or treating inflammatory pain.

"Functional food" as defined herein refers to food manufactured and processed using ingredients or ingredients having useful functionality for the human body according to Act No. 6627 of the Functional Food, and "functional" means the structure of the human body and It means to intake for the purpose of controlling nutrients for function or obtaining useful effects for health purposes such as physiological action.

When the extract according to the present invention is used as a food additive, the extract may be added as it is or used with other foods or food ingredients, and may be suitably used according to a conventional method. Examples of foods to which the above substances can be added are meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other dairy products including noodles, gums, ice cream, various soups, beverages, teas, drinks, Alcoholic beverages and vitamin complexes are included, and all of the health foods in the ordinary sense are included.

According to the composition for preventing or treating inflammatory pain derived from the present invention, inflammatory pain can be alleviated.

When used according to the composition for preventing or treating inflammatory pain, extracts derived from natural products are used to effectively relieve saliva in the oral cavity while effectively reducing inflammatory pain, thereby effectively preventing acute inflammatory pain or facial pain caused by oral diseases. Or it can be treated.

Figure 1 relates to the test results of a painful joint pain model of cinnamon extract.
Figure 2 relates to the subcutaneous pain model test results of the cinnamon extract.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art to which the present invention pertains can easily practice. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein.

[Production Example 1: Preparation of composition for preventing or treating inflammatory pain]

1. Preparation of cinnamon extract

After finely drying the dried cinnamon with a hand mixer, the cinnamon was shaken with 5 times the volume of ethanol (v/v) for 24 hours, extracted repeatedly 5 times, and then concentrated under reduced pressure to prepare a cinnamon ethanol extract.

2. Preparation of natural extracts

In the same manner as the cinnamon extract, Gaenja extract, Eumjigye extract and Sambaekcho extract were prepared.

3. Preparation of complex extracts

The cinnamon extract, Gaebija tree extract, Eumji cigarette extract and Sambaekcho extract were mixed in a weight range as shown in Table 1 below to prepare a composition for preventing or treating inflammatory pain.

V1 V2 V3 V4 V5 V6 cinnamon 100 100 100 100 100 100 Gavin Tree - 30 40 50 60 70 A dark palace - 30 40 50 60 70 Three hundred seconds - 30 40 50 60 70

(Unit weight part)

[Experimental Example 1: Formalin pain model test for cinnamon extract]

One. TMJ (temporomandibular joint) model

TMJ (temporomandibular joint) model (temporomandibular joint model, Won KA, Kang YM, Lee MK, Park MK, Ju JS, Bae YC, et al. 2012; 26(2): 132-141.) was used to conduct an acute temporomandibular pain relief effect experiment on cinnamon ethanol extract and the results are shown in FIG. 1 below.

When explaining a specific experimental method, the experimental animal was adapted for 10 minutes or more in an experimental plastic container before evaluating the pain response. Insulin syringe (0.25 × 8 mm) was used to inject 30 μl of 5% formalin, and it was observed to restore consciousness within a few seconds after the formalin injection.

The position of the joint cavity was inferred by palpation of the posterior lower boundary surface of the tibia and the mandibular condyle area, and the injection needle pierced the joint sac in the affected area and recognized the area of contact with the mandible into the joint cavity. Preliminary experiments confirmed the location of the temporomandibular joint cavity by injecting 1% by weight of 1% evans blue dye in the same amount as formalin. After injecting formalin, the behavioral response of rubbing or scratching the TMJ area and the facial area is regarded as a pain indicator, and cumulatively recorded for 9 consecutive minutes at 5 minute intervals for 45 minutes, and the primary pain behavioral response (0-10 minutes) and the secondary painful behavioral response (11 to 45 minutes).

Referring to FIG. 1 below, in an experiment using an animal (Rat), it is a figure showing a change in pain behavior response according to administration of cinnamon ethanol extract in an acute pain model induced with formalin administered to the temporomandibular joint. There was no significant difference between the formalin injection group, the control group (veh + formalin injection group), and the drug injection group when the cinnamon ethanol extract was administered. 25mg/kg, 50mg/kg,) all significantly reduced the pain behavior response. Through these results, it was found that cinnamon ethanol extract (V1) was effective in controlling acute pain in the temporomandibular joint.

2. SC (subcutaneous) model (facial subcutaneous pain model)

Subcutaneous (SC) model (Won KA, Park SH, Kim BK, Baek KS, Yoon DH, Ahn DK.Intracisternal Administration of Voltage Dependent Calcium Channel Blockers Attenuates Orofacial Inflammatory Nociceptive Behavior in Rats.Int J Oral Biol 2011 ; 36(2): 43-50.;Choi HS, Ju JS, Lee HJ, Kim BC, Park JS, Ahn DK.Effects of intracisternal injection of interleukin-6 on nociceptive jaw opening reflex and orofacial formalin test in freely moving rats Using Brain Res Bull 2003; 59(5): 365-370.), the effect of improving the effect on the subcutaneous pain in the face of the cinnamon ethanol extract (V1) was tested and the results are shown in FIG. 2 below.

When explaining a specific experimental method, the experimental animal was adapted for 10 minutes or more in an experimental plastic container before evaluating the pain response. 5% formalin (50 μl) was injected under the right beard of the experimental animal using an insulin syringe (0.25 m (31 G) m × mm), and the act of rubbing or scratching the face was considered as a pain indicator. The behavioral response was observed for 45 minutes by accumulating in 5 minute increments immediately after the injection, and evaluated by classifying the primary pain behavioral response (0 to 10 minutes, first phase) and the secondary painful behavior response (11 to 45 minutes, second phase). Did.

Referring to FIG. 2 below, in an experiment using animals (Rat), it is a figure showing a change in pain behavior response according to administration of cinnamon ethanol extract in an acute pain model induced with formalin administered to a beard. There was no significant difference between the formalin infusion group, the control group (veh + formalin infusion group), and the drug infusion group during the Boswellia administration, but the secondary pain behavior response compared to the formalin infusion group compared to the formalin infusion group ( 25mg/kg, 50mg/kg,) all significantly reduced the pain behavior response. Through these results, it was found that the cinnamon ethanol extract was effective in controlling acute pain in the face.

3. Comparison of pain model test results for complex extracts

Through the experiments of the temporomandibular joint pain model and the facial subcutaneous pain model for V1, it was confirmed that it is effective in preventing or controlling inflammatory pain.

Subsequently, the same experiment for V2 to V6 was conducted, and the results were described as V1 and exponent in order to perform relative comparison. The effect level of V1 was set as an index of 5, and the effect level of V2 to 6 was described as an index of 1 to 10, and the higher the score, the better the pain control effect.

V1 V2 V3 V4 V5 V6 Temporomandibular joint pain model 5 3 6 8 7 5 Subcutaneous pain model of the face 5 3 5 9 7 5

Table 2 is a comparison result of the pain control effect of V2 to 6 compared to V1. Compared to V1, V2 and V6 showed an equal degree of pain control effect, while V3 to 5 showed relatively good pain control effect.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concept of the present invention defined in the following claims are also provided. It belongs to the scope of rights.

Claims (6)

Contains cinnamon extract,
With respect to 100 parts by weight of the cinnamon extract
Gavinja extract 40 to 60 parts by weight;
40 to 60 parts by weight of Eumjigall extract and
It contains 40 to 60 parts by weight of Sambaekcho extract
A composition for the prevention or treatment of inflammatory pain. According to claim 1,
The inflammatory pain is acute inflammatory pain or acute facial pain caused by oral disease
A composition for the prevention or treatment of inflammatory pain. According to claim 2,
The oral disease is a disease selected from the group consisting of dental caries, gingivitis, mucosal ulcers in the oral cavity, bad breath and dry mouth.
A composition for the prevention or treatment of inflammatory pain. According to claim 1,
The extract is to extract using an extraction solvent selected from the group consisting of water, C 알코올 to C₁ lower alcohols and mixtures thereof.
A composition for the prevention or treatment of inflammatory pain. A pharmaceutical agent for preventing or treating inflammatory pain, comprising a composition for preventing or treating inflammatory pain according to any one of claims 1 to 4. A functional food for preventing inflammatory pain, comprising the composition for preventing inflammatory pain according to any one of claims 1 to 4.

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