KR101704589B1 - Composition comprising extracts of Magnolia flower and Magnolia officinlis for preventing or treating periodentitis as an active ingredient - Google Patents

Abstract

The present invention relates to a pharmaceutical composition comprising an extract of magnolia officinalis with an extract of magnolia liliflora as active ingredients for stimulating growth of dental root and peridental tissues, or for preventing or treating periodontitis. Particularly, it is shown that a mixture of an extract of magnolia officinalis with an extract of magnolia liliflora inhibits activity of nitric oxide (NO) derived with LPS in Raw264.7 cells, inhibits activity of tartrate-resistant acid phosphatase (TRAP) to suppress differentiation of osteoclasts, and increases activity of alkaline phosphatase (ALP) in MC3T3 cells to stimulate differentiation of osteoblasts. In addition, it is shown that the mixture shows a high cell growth rate and ALP activity in human peridental ligament cells, and stimulates growth of dental roots and peridental tissues abstracted from mice. Thus, the mixture of an extract of magnolia officinalis with an extract of magnolia liliflora according to the present invention can be used advisably as a composition for stimulating growth of dental roots/ peridental tissues, a pharmaceutical composition for preventing or treating periodontitis, a quasi-drug composition for stimulating growth of dental roots/ peridental tissues or improving periodontitis, a health-aid food for stimulating growth of dental roots/ peridental tissues or improving periodontitis, and a cosmetic composition for stimulating growth of dental roots/ peridental tissues or improving periodontitis.

Description

[0001] The present invention relates to a pharmaceutical composition for promoting periodontal growth and preventing or treating periodontal disease, which contains as an active ingredient,

The present invention relates to a pharmaceutical composition for promoting periodontal growth and preventing or treating periodontal disease, which comprises a mixture of Magnolia officinlis and Magnolia flower as an active ingredient.

Periodontal tissue is a complex organ containing both soft and hard tissues consisting of gingiva (gums), cementum, periodontal ligament and alveolar bone. The gingiva is a simple connective tissue involved in the supporting and chewing process of the teeth, whereas the periodontal ligament is located between the teeth and the alveolar bone to support the teeth to the jaw, to resist mechanical stresses in various directions such as masticatory force, Is a highly differentiated cellular connective tissue. These periodontal ligaments are composed of osteoblasts and osteoclasts on the alveolar bone, fibroblasts on the center of periodontal ligaments, epithelial rests of Malassez, macrophages, undifferentiated mesenchymal cells, And vascular endothelial cells; And cementoblasts located on the root side, and are known to be involved in restoration and regeneration of alveolar bone, root and cementum.

Periodontal disease refers to the inflammatory state of the tooth supporting tissues caused by bacteria, which can be separated into gingivitis and periodontitis. The cause of the disease is due to the poor oral hygiene state that oral bacteria bite to form a bacterial membrane. Tooth surface bacteria is a bacterial mass that grows after the bacteria stick to the surface of the teeth by using a sticky substance in the needle as an adhesive. If you do not leave the bacterial membrane in the tooth, inflammation occurs, sometimes gum bleeding, bad breath, and sometimes the symptoms of gingivitis is called.

As the gingivitis develops further, the gap between the tooth and the gum becomes deeper, resulting in a periodontal pouch, where germs that cause periodontal disease multiply and periodontitis occurs. As periodontitis progresses, mild irritation, such as brushing, may cause bleeding from the gums and swelling, often causing acute inflammation and pain. Such inflammation lowers the function of making bone and increases the action of absorbing bone, so that the alveolar bone is gradually lowered, causing the alveolar bone to be destroyed and eventually to lose teeth. Progressive periodontitis causes tooth loss. It is reported that the percentage of periodontal disease among the causes of tooth extraction in adults aged 40 years or older is as high as 40%, and tooth loss caused by periodontitis is a serious problem.

The destruction of periodontal ligament and alveolar bone in periodontal disease is due to the degradation of collagen, which is the main component of these tissues. Although the precise mechanism for this is not yet known, collagen degradation is known to be caused by neutral proteases secreted from host-secreted collagenases and periodontal disease-causing bacteria. Therefore, studies have been made to inhibit the progression of periodontal disease by inhibiting the activities of collagenase and protease.

Treatment of periodontal disease has been based on the establishment of improved oral hygiene in patients, non-surgical or surgical calculus removal, root resorption, gingival curettage, and regeneration of periodontal tissue using neoadjuvant. However, these surgical treatment methods are difficult to effectively treat due to the hassle of going to the dentist for treatment, and they are limited to the treatment that is performed when the disease has progressed to some extent rather than the prevention of the disease. . The use of systemic antibiotics and local controlled release agents have been used as adjunctive therapies. However, there has been reported a case in which periodontal disease bacteria, which are resistant to antibiotics recently used, There is a serious problem. If the tooth is lost due to periodontal disease, the burden of the patient will be increased due to the prosthodontic treatment or the dental implant treatment. In particular, it can become a higher medical barrier for socioeconomically vulnerable people. Thus, if the damaged tissue can be regenerated before the tooth loss, the economic and social burden can be alleviated by reducing the demand for treatments such as artificial teeth or prosthesis following tooth loss.

In order to overcome the limitations of surgical treatment and the problems of antibiotic use and to improve the prevention and treatment effect of periodontal disease, it is urgently required to develop a preparation having the effect of restoring the damaged and lost periodontal tissue. Because periodontal tissues are different from each other in their constitutional characteristics, the treatment for regenerating lesions that have been destroyed by periodontal disease does not yet show promising results. Although periodontal disease enters the healing phase through non-surgical or surgical removal of inflammatory connective tissue such as plaque and calculus, the loss of periodontal tissue does not lead to regeneration of individual components of periodontal tissue Proliferation of epithelial tissue results in incomplete healing.

It is known that two types of cells are involved in aggregate formation. One of the cells is osteoblast that produces bone, and the other is osteoclast that destroys bone. These cells are derived from different cells, and mesenchymal stem cells are differentiated into cells of osteoblast, myoblast, adipocyte, fibroblast, chondrocyte, etc. And hematopoietic stem cells are differentiated into osteoclasts, macrophages, T cells, B cells, dentritic cells, basophiles, ), Eosinophile, neutrophile, platelet, red blood cell and the like. Run-2 (Runt-related transcription factor 2), MyoD (myoepithelial cell differentiation factor), PPARγ (peroxisome proliferator-activated receptors γ), and Sox9 (Exp. Cell Res., 315 (13), pp. 2231-40, 2009, Charles A. et al., Exp Cell Res., 300 2, pp. 406-17, 2004, David V. et al., Endocrinology., 148 (5), pp.2553-62, 2007, Hill TP. Et al., Dev Cell., 8 , J. Biol. Res., 23 (6), pp.939-48, 2008, Lengner CJ et al., J Biol Chem., 280 (16) pp.15872-9, 2005). In addition, Sox9, which is a major factor of chondrocyte differentiation, inhibits the maturation and differentiation of osteoblasts. Wnt, which is a differentiation factor of osteoblast, promotes Runx2 but inhibits Sox9 by degrading osteoblast. Osterix, (1), 2008, Tominaga H, et al., "Diseases of the Gastric Cancer Cell Line," Proc. et al., J Bone Miner Metab., 27 (1), pp. 36-45, 2009).

Osteoblasts produce RANKL (Receptor activator of NF-κB ligand) and its inducible receptor (decoy receptor), OPG (Osteoprotegerin). When RANKL binds to the receptors RANK (Receptor activator of NF-κB) on the surface of osteoclast progenitor cells, osteoclast precursor cells are maturated into large polynuclear osteoclasts and bone resorption occurs. However, when OPG binds to RANKL, the binding between RANKL and RANK is blocked, resulting in inhibition of osteoclast formation and no more bone resorption than necessary (Theill LE et al., Annu Rev Immunol., 20, pp. 795-823 , 2002; Wagner EF. Et al., Curr Opin Genet Dev., 11, pp. 527-532, 2001). The activity of these osteoclasts results in the absorption or destruction of old bone, which is used to maintain bodily function by piercing the bone and releasing a small amount of calcium into the bloodstream (William J. et al., Nature 423, pp. 3737342, 2003).

On the other hand, the osteoblasts generated from bone cells fill holes with collagen and cover the hydroxyapatite of calcium and phosphorus to form new hard bones (Stains JP et al., Birth Defects Res C Embryo Today. 75 (1), pp. 72-80, 2005). It takes about 100 days for the bones to begin to break down and rebuild into new bones (Schwarz EM. Et al., Curr Opin Orthop., 11, pp. 329-335, 2000). In infants, bone calcium is 100% changed within a year, but about 10-30% of the bone is reshaped every year in adults, and the osteopenic rate and osteotomy speed are the same so that bone density can be maintained as before. These two processes, namely, bone resorption and bone formation, are closely linked to each other and are important for maintaining the normal structure of the bone.

Inflammation is a deposition of collagen, calcium, and phosphorus, which weakens the ability of osteoblasts to make hard bone, and destroys or absorbs osteoclast bones. As a result, the alveolar bone is gradually lowered and destroyed, eventually resulting in tooth loss. It is known that osteoclasts are derived from monocyte / macrophage lineage cells in bone marrow, these mononuclear progenitor cells circulate in the blood, and progenitor cells proliferate in the endosteal layer and are fused to form polynuclear cells. It is known that TRAP, which is characteristic of acid phosphatase resistant to tartrate, is used as a cytochemical labeling enzyme for osteoclasts which can be distinguished from other bone tissue cells (Korean 10-2006-0092651).

The periodontal ligament is a layer with an average thickness of 0.2 mm, which attaches to the teeth and attaches and supports the teeth to the alveolar bone. In addition, periodontal ligaments disperse the force to the alveolar bone when the tooth chews the food to buffer and transmit the sense of the tooth to the alveolar bone. Because there are undifferentiated mesenchymal cells in the periodontal ligament, bone reconstruction can be continued without changing the overall structure and function. The undifferentiated mesenchymal cells in the periodontal ligament migrate toward the alveolar bone and modify the alveolar bone, or move toward the teeth to produce the collagen fibers to firmly support the teeth (Korean Patent No. 10-2016-0016127).

The Magnolia flower is the word that leads to the peaks of magnolia flowers belonging to Magnoliaceae and is called ginseng because the flower peaks taste a little spicy. In Korea, dried Magnolia denudata Desrousseaux or flower peaks are used in Korea. In Japan, M. denudata Desr., M. flower Pamp., M. korea, M. korea, Kobus DC., M. salicifolia Maxim., and M. sprengeri Pamp. In China, M. denudata Desr, M. flower Pamp., And M. sprengeri Pamp. Are used. It has been reported that Shin-yi has the properties of warmth and is effective for headache, low back pain and rhinitis, and also treats sinusitis and has the weakness of analgesic.

Magnolia (Magnolia officinlis) is a magnolia Magnolia officinalis Rehd magnolia and plants. It dried the roots and roots of et Wils. Peel off the bark of trees that have grown more than 20 years in the spring, dry them in the shade, dip them in boiling water for a while, dry them, dry them in the sun, steam them in steam again and roll them in a cylindrical shape and dry them in the sun. It has been shown that the pharmacological test shows antimicrobial activity and weak diuretic effect. It has been known to be effective in digestive disorders, vomiting, diarrhea, gastroenteritis, stomach cramps, coughing and breathing, bronchitis and bronchial asthma.

US patent application Ser. No. 13 / 739,340 relates to a pharmaceutical composition for promoting osteogenesis, wherein extracts obtained from magnolia or plant leaves or bark have the effect of promoting bone mineral density and bone growth without side effects Lt; / RTI > Korean Patent Registration No. 10-0204165 also relates to a composition for promoting bone tissue regeneration containing an extract of Zygomycetum, and discloses that Zygomycetes extract has an effect of promoting regeneration of injured bones such as osteotomy, osteoclast, There has been no report on the ability to promote periodontal growth and the treatment of periodontitis in the combination of rosemary extract and ginseng extract.

On the other hand, tooth formation, unlike the osteogenesis process, occurs at the 6th week of birth, the surface epithelium enters the inside, the cells begin to multiply in the internal connective tissue, and the cells embedded in the epithelium As they grow increasingly, the tissue of the dimension is formed below. The epithelial cells are differentiated into enamel cells that enclose the outer surface of the later teeth, and the ancestral cells and dendritic cells that form the dentin are differentiated inside. As described above, bone formation and tooth formation are completely different from each other in the involved cells and mechanism.

In this study, we investigated the effect of periodontal ligament on osteoblast differentiation, osteoclast activation, and osteoblast differentiation, while inhibiting periodontal tissue loss by periodontitis and promoting regeneration of periodontal tissue. , Root and root tissue growth, and thus the present invention has been completed.

It is an object of the present invention to provide a composition for promoting growth of root / periodontal tissue comprising a mixture of Magnolia officinlis extract and Magnolia flower extract as an active ingredient, a pharmaceutical composition for the prevention or treatment of periodontitis, A quasi-product composition for promoting tissue growth or improving periodontal disease, a health functional food for promoting root / periodontal tissue growth promotion or improving periodontal disease, and a cosmetic composition for promoting root / periodontal tissue growth or periodontal disease improvement

In order to achieve the above object, the present invention provides a pharmaceutical composition for promoting root growth or periodontal growth promotion, or preventing or treating periodontal disease, comprising a mixture of an extract of Lilac and a ginseng extract as an active ingredient.

The present invention also provides a quasi-drug composition for promoting the growth of a root or periodontal tissue containing a crude extract and a ginseng extract as an active ingredient, or for preventing or improving periodontitis.

The present invention also provides a health functional food for accelerating the growth of root or periodontal tissue or improving periodontal disease, which comprises a mixture of an aftercooled extract and a ginseng extract as an active ingredient.

In addition, the present invention provides a cosmetic composition for promoting the growth of root or periodontal tissue or improving periodontal disease, which comprises a mixture of an aftercooled extract and a ginseng extract as an active ingredient.

The inventive extract of the present invention inhibited the activity of nitric oxide (NO) induced by LPS in Raw264.7 cells and suppressed osteoclast differentiation by inhibiting TRAP (Tartrate-resistant acid phosphatase) Increasing ALP (alkaline phosphatase) activity in MC3T3 cells promoted osteoblast differentiation. In addition, it was confirmed that the human periodontal ligament cells exhibited excellent cell proliferation and ALP activity, and confirming that the root and trabecular tissue growths extracted from the mice were promoted, the mixture of the Chinese cabbage extract and Shin- A composition for growth promotion, a pharmaceutical composition for preventing or treating periodontitis, a quasi-composition for promoting root growth / periodontal tissue growth or improving periodontal disease, a health functional food for improving root / periodontal tissue growth or improving periodontal / periodontal tissue growth Or as a cosmetic composition for improving periodontitis.

1 is a graph showing the inhibitory effect of nitric oxide (NO) on Raw 264.7 cells induced by a mixture of the extract and the extract.
FIG. 2 is a graph showing the effect of inhibiting TRAP (Tartrate-resistant acid phosphatase) activity in Raw 264.7 cells by a mixture of an extract of Lilac and a ginseng extract.
FIG. 3 is a graph showing the effect of increasing alkaline phosphatase (ALP) activity in MC3T3-E1 cells by the mixture of the extract of Leek extract and Shin extract.
FIG. 4 is a chart for confirming the cell proliferation rate in human periodontal ligament cells by the mixture of the extract of Leek extract and Shin extract.
FIG. 5 is a graph showing ALP activity in human periodontal ligament cells by the mixture of the extract of Leek extract and Shin extract.
FIG. 6 is a graph showing the effect of the mixture of the extract of Lycoris spp. And the extract of Shin extract on the growth of root and periodontal tissues of mice.

Hereinafter, the present invention will be described in detail.

The present invention provides a pharmaceutical composition for promoting periodontal growth or preventing or treating periodontal disease, which comprises a mixture of an aftercooled extract and a ginseng extract as an active ingredient.

It is preferable that the mixture of the backbone extract and the Shin extract is mixed at a concentration ratio of 1: 1 to 1:50, more preferably, but not necessarily, at a concentration ratio of 1: 3 to 1:30.

The mixture is preferably, but not limited to, inhibiting nitric oxide (NO) activity, inhibiting TRAP (tartrate-resistant acid phosphatase) activity, and increasing ALP (alkaline phosphatase) activity.

In a specific example of the present invention, when Raw.264.7 cells were treated with a single extract of L. extract or Shin extract, or a mixture of L. extract and Shin extract at a ratio of 1: 3, 1: 10 or 1: (Fig. 1) inhibited nitric oxide (NO) activity induced by LPS (lipopolysaccharide) (see FIG. 1), and increased RAP.264.7 TARP (Tartrate-resistant acid phosphatase) activity by RANKL treatment (Fig. 2), and it was confirmed that osteoclast differentiation was inhibited. The osteoclast differentiation inhibitory effect of osteoclast differentiation was compared with that of each extract alone, 1, and 1: 30, respectively. The mixture was found to have the most effective osteoclast differentiation inhibitory effect (see FIGS. 1 and 2).

In addition, the present inventors confirmed that the inhibition of ALP (alkaline phosphatase) activity in MC3T3-E1 cells was effective in promoting osteoblast differentiation (see FIG. 3) Compared with single treatment, it was confirmed that the most effective osteoblast differentiation promoting effect was obtained in a mixture in which the extract of Puerariae Radix and Shin extract was mixed at a ratio of 1:10.

In addition, the present inventors have confirmed the effects of the extract of L. and Shin extract on human periodontal ligament cell proliferation, cell activity, and differentiation degree. As a result, when the L. extract or Shin extract is present alone, (See Fig. 4). This result was confirmed by the fact that the two extracts showed synergistic effect when the extract of L. extract and Shin extract were mixed together.

In addition, the present inventors confirmed that the extract of L. extract and Shinkai extract increased the ALP activity. As a result, it was found that when the extract was treated alone with the mixture of L. extract and Shin extract at a mixing ratio of 1:10, ALP Activity was increased by about 40% or more (see FIG. 5).

In addition, the inventors of the present invention found that the mouse lower mandibles were embedded in agarose beads containing the extract of Guava extract and Shin extract, and then transplanted into a host mouse. After 3 weeks, root and periodontal tissue growth were observed. In the control group, The growth of root and periodontal tissues was negligible during the treatment with the extract of L. or Shin extract but the treatment of mixture of L. and Shin extract increased the periodontal tissue growth. And the alveolar bone formation around the implanted teeth was the most excellent (see FIG. 6).

Therefore, the mixture of the extract of Liliaceae and the extract of Liliaceae of the present invention can be usefully used as a pharmaceutical composition for promoting root growth or periodontal growth and for the prevention or treatment of periodontitis.

The composition of the present invention may contain, as an active ingredient, 0.1 to 99.9% by weight of a mixture of the nutmeg and extract of the present invention with respect to the total weight of the composition, and may include a pharmaceutically acceptable carrier, excipient or diluent.

The pharmaceutically acceptable carrier may be a mixture of saline, sterilized water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, and one or more of these components. If necessary, an antioxidant, Other conventional additives such as a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into main dosage forms such as aqueous solutions, suspensions, emulsions, powders, tablets, capsules, rings, granules or injection solutions. Further, it can be suitably formulated according to each disease or ingredient, using the method disclosed in Remington's Pharmaceutical Science (Mack Publishing Company, Easton PA, 18th, 1990) in a suitable manner in the art.

The pharmaceutical composition for the prevention and treatment of oral diseases of the present invention can be formulated into tablets, capsules, powders, granules, suspensions, emulsions, syrups and other liquid preparations by a conventional method. Specifically, the pharmaceutical compositions of the present invention can be formulated for oral administration, for example, as tablets, troches, lozenges, aqueous or aqueous suspensions, prepared powders or granules, emulsions, hard or soft capsules, It is formulated into elixirs. Binders such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin, excipients such as dicalcium phosphate, disintegrants such as corn starch or sweet potato starch, disintegrants such as starch, , Calcium stearate, sodium stearyl fumarate, or polyethylene glycol wax. In the case of a capsule formulation, in addition to the above-mentioned substances, a liquid carrier such as fatty oil is contained.

In addition, the pharmaceutical composition of the present invention can be administered orally or parenterally, and it is preferable to select subcutaneous injection, intravenous injection, intramuscular injection, or intra-thoracic injection injection method for parenteral administration. For formulation into parenteral administration formulations, Compound 1 of the present invention is mixed with water to prepare a suspension, which is then formulated in unit dosage forms of ampoules or vials.

The dosage of the active ingredient according to the present invention is appropriately selected depending on the degree of absorption, inactivation rate and excretion rate of the active ingredient in the body, the age, sex and condition of the patient, and severity of the disease to be treated. The compound 1 of the present invention may be administered to an adult in an amount of 0.0001-500 mg per 1 kg of body weight per day, preferably in an amount of 0.001-100 mg. The composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers.

The present invention also provides a quasi-drug composition for promoting the growth of a root or periodontal tissue containing a crude extract and a ginseng extract as an active ingredient, or for preventing or improving periodontitis.

The quasi-drug composition is preferably an oral composition such as a toothpaste, an oral cleanser, or an oral cleanser, but is not limited thereto.

It is preferable that the mixture of the backbone extract and the Shin extract is mixed at a concentration ratio of 1: 1 to 1:50, more preferably, but not necessarily, at a concentration ratio of 1: 3 to 1:30.

Preferably, the mixture inhibits nitric oxide (NO) activity, inhibits TRAP (tartrate-resistant acid phosphatase) activity, and increases ALP (alkaline phosphatase) activity.

In a specific embodiment of the present invention, the combination of rosemary extract and ginseng extract inhibits nitric oxide (NO) activity by LPS (lipopolysaccharide) (see FIG. 1) and increased RAP 264.7 TRAP Tartrate-resistant acid phosphatase activity was effectively inhibited (see FIG. 2), and it was confirmed that osteoclast differentiation was inhibited. The extract of L. elata and Shin extract was mixed at a ratio of 1:10 and 1:30 (Fig. 1 and Fig. 2).

In addition, the present inventors confirmed that the inhibition of ALP (alkaline phosphatase) activity in MC3T3-E1 cells was effective in promoting osteoblast differentiation (see FIG. 3) Concentration ratio, the most effective osteoblast differentiation promotion effect was confirmed.

In addition, the present inventors have confirmed the effects of the extract of L. and Shin extract on human periodontal ligament cell proliferation, cell activity, and differentiation degree. As a result, when the L. extract or Shin extract is present alone, (See Fig. 4). This result was confirmed by the fact that the two extracts showed synergistic effect when the extract of L. extract and Shin extract were mixed together.

In addition, the present inventors confirmed that the extract of L. extract and Shinkai extract increased the ALP activity. As a result, it was found that when the extract was treated alone with the mixture of L. extract and Shin extract at a mixing ratio of 1:10, ALP Activity was increased by about 40% or more (see FIG. 5).

In addition, the present inventors increased the periodontal tissue growth by confirming the root and periodontal tissue growth after embedding the lower jaw molars into the agarose beads containing the extract of Zucchini and Shin extract, And it was confirmed that the root growth and the alveolar bone formation around the implanted tooth were the best at a mixture concentration ratio of 1:10 (see FIG. 6).

Therefore, the mixture of the extract of Liliaceae and the extract of Liliaceae of the present invention can be usefully used as a composition for promoting growth of root or periodontal tissue or quasi salts for improving periodontal disease.

The composition of the present invention, which is a quasi-drug composition containing a crude extract and a mixture of a ginseng extract, is not particularly limited in its formulation and may have conventional formulations. Specifically, it may contain a formulation such as a toothpaste, mouthwash or mouthwash have. The oral composition provided in the present invention may contain various bases and additives necessary for formulation according to the formulation, and the kind and amount of these ingredients can be easily selected by those skilled in the art. For example, when the oral composition is a dentifrice, it may be prepared by adding an abrasive, a wetting agent, a foaming agent, a binder, a sweetener, a pH adjuster, a preservative, a medicinal ingredient, a flavoring agent, a brightener, a colorant and a solvent.

The present invention also provides a health functional food for accelerating the growth of root or periodontal tissue or improving periodontal disease, which comprises a mixture of an aftercooled extract and a ginseng extract as an active ingredient.

It is preferable that the mixture of the backbone extract and the Shin extract is mixed at a concentration ratio of 1: 1 to 1:50, more preferably, but not necessarily, at a concentration ratio of 1: 3 to 1:30.

The mixture is preferably, but not limited to, inhibiting nitric oxide (NO) activity, inhibiting TRAP (tartrate-resistant acid phosphatase) activity, and increasing ALP (alkaline phosphatase) activity.

In a specific embodiment of the present invention, the combination of rosemary extract and ginseng extract inhibits nitric oxide (NO) activity by LPS (lipopolysaccharide) (see FIG. 1) and increased RAP 264.7 TRAP Tartrate-resistant acid phosphatase activity was effectively inhibited (see FIG. 2), and it was confirmed that osteoclast differentiation was inhibited. The extract of L. elata and Shin extract was mixed at a ratio of 1:10 and 1:30 (Fig. 1 and Fig. 2).

In addition, the present inventors confirmed that the inhibition of ALP (alkaline phosphatase) activity in MC3T3-E1 cells was effective in promoting osteoblast differentiation (see FIG. 3) Concentration ratio, the most effective osteoblast differentiation promotion effect was confirmed.

In addition, the present inventors have confirmed the effects of the extract of L. and Shin extract on human periodontal ligament cell proliferation, cell activity, and differentiation degree. As a result, when the L. extract or Shin extract is present alone, (See Fig. 4). This result was confirmed by the fact that the two extracts showed synergistic effect when the extract of L. extract and Shin extract were mixed together.

In addition, the present inventors confirmed that the extract of L. extract and Shinkai extract increased the ALP activity. As a result, it was found that when the extract was treated alone with the mixture of L. extract and Shin extract at a mixing ratio of 1:10, ALP Activity was increased by about 40% or more (see FIG. 5).

In addition, the present inventors increased the periodontal tissue growth by confirming the root and periodontal tissue growth after embedding the lower jaw molars into the agarose beads containing the extract of Zucchini and Shin extract, And it was confirmed that the root growth and the alveolar bone formation around the implanted tooth were the best at a mixture concentration ratio of 1:10 (see FIG. 6).

Therefore, the mixture of the extract of the present invention and the ginseng extract of the present invention can be useful as a health functional food for promoting root growth or periodontal tissue growth or improving periodontal disease.

The health functional food of the present invention may contain various flavors or natural carbohydrates as an additional ingredient. The above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau Martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate may be selected from the range of 0.01 to 0.04 part by weight, specifically about 0.02 to 0.03 part by weight per 100 parts by weight of the health functional food of the present invention.

In addition to the above, the health functional food of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, protective colloid thickener, pH adjuster, stabilizer, preservative, glycerin, A carbonating agent used in a carbonated beverage, and the like. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health food of the present invention.

There is no particular limitation on the kind of the food. Examples of the food to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include healthy foods in a conventional sense.

In addition, the present invention provides a cosmetic composition for promoting the growth of root or periodontal tissue or improving periodontal disease, which comprises a mixture of an aftercooled extract and a ginseng extract as an active ingredient.

It is preferable that the mixture of the backbone extract and the Shin extract is mixed at a concentration ratio of 1: 1 to 1:50, more preferably, but not necessarily, at a concentration ratio of 1: 3 to 1:30.

The mixture is preferably, but not limited to, inhibiting nitric oxide (NO) activity, inhibiting TRAP (tartrate-resistant acid phosphatase) activity, and increasing ALP (alkaline phosphatase) activity.

In a specific embodiment of the present invention, the combination of rosemary extract and ginseng extract inhibits nitric oxide (NO) activity by LPS (lipopolysaccharide) (see FIG. 1) and increased RAP 264.7 TRAP Tartrate-resistant acid phosphatase activity was effectively inhibited (see FIG. 2), and it was confirmed that osteoclast differentiation was inhibited. The extract of L. elata and Shin extract was mixed at a ratio of 1:10 and 1:30 (Fig. 1 and Fig. 2).

In addition, the present inventors confirmed that the inhibition of ALP (alkaline phosphatase) activity in MC3T3-E1 cells was effective in promoting osteoblast differentiation (see FIG. 3) Concentration ratio, the most effective osteoblast differentiation promotion effect was confirmed.

In addition, the present inventors have confirmed the effects of the extract of L. and Shin extract on human periodontal ligament cell proliferation, cell activity, and differentiation degree. As a result, when the L. extract or Shin extract is present alone, (See Fig. 4). This result was confirmed by the fact that the two extracts showed synergistic effect when the extract of L. extract and Shin extract were mixed together.

In addition, the present inventors confirmed that the extract of L. extract and Shinkai extract increased the ALP activity. As a result, it was found that when the extract was treated alone with the mixture of L. extract and Shin extract at a mixing ratio of 1:10, ALP Activity was increased by about 40% or more (see FIG. 5).

In addition, the present inventors increased the periodontal tissue growth by confirming the root and periodontal tissue growth after embedding the lower jaw molars into the agarose beads containing the extract of Zucchini and Shin extract, And it was confirmed that the root growth and the alveolar bone formation around the implanted tooth were the best at a mixture concentration ratio of 1:10 (see FIG. 6).

Therefore, the mixture of the extract of Lilac and the extract of Liliaceae of the present invention can be usefully used as a cosmetic composition for promoting root growth or periodontal growth or improving periodontal disease.

The cosmetic composition may include, but is not limited to, lotions, ointments, gels, creams, patches or sprays. In preparing a cosmetic composition containing a mixture of a rosemary extract and a ginseng extract of the present invention, 3 to 30 parts by weight, preferably 5 or 20 parts by weight, of the rosemary extract and the ginseng extract of the present invention are added to a cosmetic composition usually contained in the composition By weight.

The cosmetic composition of the present invention may further contain, in addition to the mixture of the extract and the extract of the present invention, a lipid, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, A preservative, a vitamin, a barrier, a wetting agent, a essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent, a lipid vesicle or May contain adjuvants conventionally used in the field of dermatology, such as any other ingredient conventionally used in cosmetic compositions. The components can also be introduced in amounts commonly used in the field of dermatology.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

EXAMPLES The following examples and experimental examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Manufacture of ginseng extract

The present inventors extracted the flower bud of Chinese origin (purchased from Osaka cloth, Japan) for 3-7 days by adding ethanol corresponding to 10 times its weight. Thereafter, the extract was filtered to remove insoluble residue, and the filtered extract was concentrated using a vacuum concentrator.

The concentrated extract was lyophilized to obtain 300 g of a ginseng extract as a solid phase.

Preparation of extract

The present inventors extracted the husk bark (1 kg) purchased from Osaka cloth, Japan, for 3-7 days by adding ethanol corresponding to 10 times its weight. Thereafter, the extract was filtered to remove insoluble residue, and the filtered extract was concentrated using a vacuum concentrator.

The concentrated extract was lyophilized to obtain 200 g of a supernatant extract.

Manufacture of a mixture of ginger extract and ginger extract

The inventors of the present invention found that the powdery ginseng extract obtained from Example 1 and Example 2 were dissolved in DMSO (0.5, 1, 3 mg / ml, 1.2, 3, 5, 10, 15, 30 mg / ml).

For the preparation of the mixture of the crude extract and the Shin extract for use in the following <Experimental Example 1>, the ratio of the crude extract to the fresh extract was adjusted to 1: 3 (1 ㎍ / ml + fresh extract 3 ㎍ / ml) , 1:10 (1 mg / ml of glutinous extract, 10 g / ml of glutinous extract) or 1:30 (1 mg / ml of glutinous extract and 30 g / ml of glutinous extract).

For the preparation of the mixture of the extract and the extract of the present invention for use in the following <Experimental Example 2>, the concentration ratio of the extract and the extract was adjusted to 1: 3 (0.5 g / ml of Extract extract + 1.5 g / ml of Extract of Shin extract) The extracts were prepared at 1:10 (0.5 ml / ml of glutinous extract + 5% g / ml of Shin extract) or 1:30 (glutinous extract of 0.5 g / ml + Shin extract of 15 g / ml).

For the preparation of the mixture of the extract and the extract of the present invention for use in the following <Experimental Example 3>, 1: 1 (3. (3 ug / ml + ginseng extract 10 ㎍ / ml) or 1:10 (3 ug / ml + ginseng extract 30 g / ml).

< Experimental Example  1> Determination of the Effect of Mixture of Extracts and Extracts on Nitric Oxide Production

<1-1> Determination of cell survival rate by treatment with extracts of Guanxi and Shin extract

The present inventors investigated the cytotoxicity of the mixture of the extract and the extract of Shin extract by treating the extracts or the extracts prepared in Examples 1 to 3 and the mixture thereof in Raw 264.7 cells as follows Experiments were performed.

Specifically, Raw 264.7 cells were inoculated at a concentration of 3 × 10 3 cells / well in a 96-well culture dish and cultured at 37 ° C. and 5% CO 2 for 4 hours. LPS was treated with 0.01 ㎍ / ml of LPS and the extract of L. urea (0.05 되도록 in concentration of 1 / / ml in 50 배 of medium) at a concentration of 1 mg / ml obtained in <Example 2> The extracts of 3, 10 and 30 mg / ml obtained in Example 3 were diluted to 0.05 μl in 50 μl of the medium to give a concentration of 3, 10 and 30 μg / ml of Shin extract, respectively, (50 μl of the medium and 0.05 μl of the extract of Shinji) were mixed and cultured for 4 days at a concentration ratio of 1: 3, 1:10 and 1:30. The cell counting kit (Dojindo) was treated with 10 μl / well, and the absorbance was measured at 450/620 nm using a microplate reader. The cell viability was converted into a percentage by comparing with the absorbance of the control group.

As a result, it was confirmed that there was no significant cytotoxicity in the group treated with the extract of Goji and Shin extract.

<1-2> Confirmation of NO (nitric oxide) production inhibitory activity of mixture of extract and ginseng extract

The present inventors conducted the following experiments in order to confirm the effect of the mixture of the extract and the extract on the production of NO.

Specifically, Raw 264.7 cells were inoculated in a 96-well culture dish at a concentration of 3 × 10 3 cells / well, cultured at 37 ° C. in 5% CO 2 for 4 hours, treated with 0.01 μg / ml of LPS, Ml of the extract obtained in Example 2 (concentration of 1 μg / ml in 50 μl of the culture medium) (0.05 μl), the concentration of 3, 10 and 30 mg / ml obtained in Example 1 The extracts were added to each of 50 μl of the medium (the concentrations of 3, 10 and 30 μg / ml of the extract were adjusted to 0.05 μl in the medium, respectively), or the extracts of the dried and dried extracts prepared in Example 3 : 10 and 1: 30 (50 [mu] l of medium and 0.05 [mu] l of extract of Shinki) were mixed and cultured for 24 hours. The same amount of Griess Reagent (Promega) was added to the culture solution (50 ㎕), and incubated at room temperature for 10 minutes. Absorbance was measured at 540 nm by ELISA.

As a result, as shown in FIG. 1, it was confirmed that the inhibitory activity of NO production by LPS was the highest in the cells treated with the 1: 30 ratio of L. extract and Shin extract. The inhibition of NO increased by LPS at 1 ㎍ / ml and Shin extracts of 3 and 10 ㎍ / ml was inhibited by 30% inhibition of NO activity at 30 ㎍ / ml of Shin extract.

In addition, the NO activity was significantly inhibited by the mixture of the extract of L. extract and the extract of Shin extract. The ratio of 1: 3 mixture of L. and Shin extract was about 10% higher than that of 3 ㎍ / : 30 ratio mixture showed 20% inhibitory effect on NO activity compared with 30 ㎍ / ml extract of Shin extract. However, in the mixture treated with 1:10 mixture of extract and. Extract, about 50 %. The concentration ratio of 1:10 was the most effective, and it was concluded that the preparation of the mixture of the extract and the ginseng extract containing 10 ㎍ / ml of ginseng extract would be economical.

< Experimental Example  2> Confirmation of the osteoclast differentiation inhibitory effect of a mixture of acanthoxylate extract and ginseng extract

<2-1> Determination of cell survival rate by treatment with extracts of Puerariae Radix and Extracts

The present inventors investigated the cytotoxicity of the mixture of the extract and the extract of Shin extract by treating the extracts or the extracts prepared in Examples 1 to 3 and the mixture thereof in Raw 264.7 cells as follows Experiments were performed.

Specifically, Raw 264.7 cells were inoculated at a concentration of 3 × 10 3 cells / well in a 96-well culture dish and cultured at 37 ° C. and 5% CO 2 for 4 hours. The RANKL of 50 mg / ml was treated with 0.05 μl of 50 μg / ml of 50 μg / ml of the medium, and 0.5 ml of the supernatant extract obtained in Example 2, the extract of Example 1 (0.05 μl each in 50 μl of the medium so that the concentrations of the extracts of 0.5 μg / ml and 1.5 μg, 5 μg and 15 μg / ml were obtained, respectively) at the concentrations of 1.5, 5 and 15 mg / (50 μl of the medium and 0.05 μl of the extract of Shinwha and Shin extract) were mixed at a concentration ratio of 1: 3, 1:10 and 1:30, respectively, Lt; / RTI &gt; The cell counting kit (Dojindo) was treated with 10 μl / well, and the absorbance was measured at 450/620 nm using a microplate reader. The cell viability was converted into a percentage by comparing with the absorbance of the control group.

As a result, it was confirmed that there was no significant cytotoxicity in the group treated with the extract of Goji and Shin extract.

&Lt; 2-2 > TRAP ( Tartrate -resistant acid 포스화제 ) Inhibitory activity

In order to confirm the effect of the mixture of the extract and the extract on the osteoclast differentiation, Raw 264.7 cells were treated with RANKL to induce osteoclast differentiation, After treatment of the mixture, the activity of TRAP (Tartrate-resistant acid phosphatase), an osteoclast-specific gene, was measured.

Specifically, Raw 264.7 cells were inoculated at a concentration of 3 × 10 3 cells / well in a 96-well culture dish and cultured at 37 ° C. and 5% CO 2 for 4 hours. Then, RANKL (50 μg / 0.05 μl), and the extracts of 0.5 ml / ml of the extract obtained in Example 2 and the extracts of 1.5, 5 and 15 mg / ml of the extract obtained in Example 1 (0.5 μg / ml), 1.5, 5, and 15 占 퐂 / ml of Shin extract were added to each 50 占 퐇 of the extract to a concentration of 1: 3, , 1: 10 and 1: 30 (50 [mu] l of the medium and 0.05 [mu] l of the extract of Shinki and Shinchi) were cultured for 4 days. The medium was removed and 50 [mu] l / well of 10% formalin solution was added and fixed for 25 minutes. After removing the 10% formalin solution and washing with 100 μl of 100% ethanol, the plate was dried for 1 day and treated with 100 μl / well of TRAP assay buffer containing p-Nitrophenyl phosphate at a concentration of 1.36 mg / ml . After reacting for 1 hour, 85 μl of the reaction solution was mixed with the same amount of 0.1 N NaOH, reacted in a new culture dish, and absorbance was measured at 405/620 nm.

As a result, as shown in FIG. 2, TRAP inhibitory activity was not exhibited at a concentration of 0.5 μg / ml of extract of Bean curd extract and 1.5 and 5 μg / ml of Shin extract. However, TRAP inhibitory effect of about 20% Respectively. However, it was confirmed that the mixture of the extract of Phellinus linteus extract and the extract of Shin extract was mixed at a ratio of 1: 3, 1:10, 1: 30, and the TRAP inhibitory effect was better than that of the extract of Shin extract (15 ㎍ / ml alone).

The ratio of 1: 3 ratio mixture of L. extract and Ginseng extract was about 25% higher than that of Shin extract at 1.5 ㎍ / ml and that of L. extract and Shin.i extract at 1: 30% TRAP inhibitory effect, but the 1:10 ratio mixture of L. extract and Shin extract showed about 50% inhibition of TRAP activity compared with 5 ㎍ / ml extract of Shin extract. : 10 was the most effective, and it was concluded that it would be economical to prepare a mixture of the extracts of Phellodendri acerbacterium L. and Shin extract containing 10.

In addition, TRAP activity was not inhibited by the addition of 0.5 ㎍ / ml of extract or 0.5 to 5 ㎍ / ml of Shin extract. However, 1: 3 (0.5 ㎍ / ml of glutinous extract plus 1.5 ㎍ / ml of Shin extract) Or 1:10 (0.5 μg / ml of glutamine plus 5 μg / ml of glutamine) inhibited TRAP activity. The ratio of 1: 30 inhibited the osteoclast differentiation, Were synergistic against osteoclast inhibition. In particular, 1:10 mixture treatment showed about 50% inhibition of TRAP activity, and it was not significantly different from 1:30 ratio mixing treatment. It was confirmed that the osteoclast differentiation inhibitory effect was excellent (Fig. 2).

< Experimental Example  3> Confirmation of osteoblast differentiation inducing effect of mixture of extract

To determine the osteoblast differentiation inducing effect of the extracts of Puerariae Radix and Shin extract, alkaline phosphatase (ALP) activity, which is a marker of osteoblast differentiation, was measured in MC3T3-E1 cells.

Specifically, MC3T3-E1 cells were inoculated in a 96-well culture dish at a concentration of 5 × 10 3 cells / well, followed by incubation at 37 ° C. and 5% CO 2 for 2 days. The topical extract at a concentration of 3 mg / ml obtained in Example 2, the Shin extract at the concentrations of 3, 10 and 30 mg / ml obtained in Example 1, or the topical extract prepared in Example 3 And gins were mixed at a concentration ratio of 1: 1, 1: 3, and 1:10, respectively, to a concentration of 50 μl / well. After the medium was replaced with 150 μl / well of the culture medium containing the induction factor, Respectively. After removing the medium and washing with 100 μl of 100% methanol, the culture dish was dried for 1 day, treated with 100 μl / well of ALP assay buffer containing p-Nitrophenyl phosphate at a concentration of 2.47 mg / ml, The absorbance was measured at 405/620 nm using a microplate reader.

As a result, as shown in FIG. 3, when 3 μg / ml of extract of Extendant extract or 3, 10 and 30 μg / ml of Extendant extract were treated alone, the ALP activity was increased about 20% (30 ㎍ / ml), the extracts were treated with a mixture of the extracts of L. and L. extracts at a mixing ratio of 1:10. The ALP activity was increased by more than 50% . Therefore, it was confirmed that a mixture of the mixture of the extract and the ginseng extract, particularly at a ratio of 1:10, promoted osteoblast differentiation (FIG. 3).

< Experimental Example  4> Man of mixture of ginger extract and ginger extract Periodontal ligament cells  Identification of proliferation and cell activity

<4-1> Acacia extract  And Ginseng extract Periodontal ligament cells  Confirmation of proliferation effect

The present inventors conducted the following experiments in order to investigate the effects of the extracts of the Chinese cabbage extract and Shin extract on the growth of the human periodontal ligament cells and the degree of cell activity increase and differentiation obtained in the above Examples 1 to 3 .

Specifically, the portion of the periodontal ligament of the premolars extracted for the purpose of orthodontic treatment for healthy persons was aseptically scratched using a curret, placed on a culture dish, and cultured with 10% FBS (Fetal Bovine Serum) Respectively. The culture was exchanged at intervals of 3 days and cultured up to 5 passages and used for the experiment. During the culture, the culture was continued by supplying 95% humidity, 37C, and 5% CO ₂ . To confirm the effect of periodontal ligament cell proliferation, ³ × 10 ³ cells / well were inoculated in a 96-well plate, followed by incubation for 2 days. After 2 days, 100 μl of the confluent cells were treated with the extract of 1 mg / ml of the extract obtained in Example 2 and the extract of Shin extract of 1, 3 and 10 mg / ml obtained in Example 1 / ml, and treated with 0.05 μl each of 50 μl of the medium so that the concentrations of 1, 3, and 10 μg / ml of the extract were in the range of 1: 1, 1: 1, 1: 3 and 1:10, and the mixture was incubated for 4 days. After incubation, 20 μl of MTT (methyl thiazol-2-YL-2,5-diphenyl tetraolium bromide, Sigma) dissolved in PBS was added to each well and incubated for 4 hours. The MTT solution was removed and the formazon crystals were dissolved 100 [mu] l / well were added to each well. Plate was shaken well and absorbance was measured at 405/620 nm using a microplate reader.

As a result, as shown in FIG. 4, when the complex was present at a ratio of 1:10 as compared with when the extract was extracted singly, the extract showed remarkably high cell activity (FIG. 4). This result was confirmed by the fact that the two extracts showed synergistic effect when the extract of L. extract and Shin extract were mixed together.

<4-2> Acacia extract  And Ginseng extract Periodontal ligament cells  Check ALP activity

ALP (Alkaline phosphatase, ALP) is known as an early indicator of periodontal ligament cell differentiation. Thus, the present inventors assessed whether the pasta and ginseng extract complexes increased ALP activity.

Specifically, the cultured cells were inoculated into each well of a 96-well plate at a concentration of ³ × 10 ³ cells / well and cultured at 37 ° ^C and 5% CO ₂ for 2 days. 100% confluent cells were cultured in the same manner as in Example 2 except that the extract of 1 mg / ml of the extract obtained in Example 2 and the extract of Shin extract of 1, 3 and 10 mg / ml obtained in Example 1 1, 3 and 10 쨉 g / ml of the extract were treated with 0.05 쨉 l each of 50 쨉 l of the medium so as to obtain the concentration of the extract, And 50 μl of a mixture (50 μl) mixed at a concentration ratio of 1:10 and 150 μl / well of a culture medium containing differentiation inducing factors (10 mM beta-glycerophosphate (Sigma), 50 μg / ml L-ascorbic acid After removing the medium and washing with 100 μl of 100% methanol, the culture dish was dried for 1 day and ALP assay buffer containing 2.47 mg / ml p-Nitrophenyl phosphate was added to 100 After incubation for 1 hour, the absorbance was measured at 405/620 nm using a microplate reader.

As a result, as shown in FIG. 5, ALP activity was increased about 10% at 10 ㎍ / ml of the extract of Shin extract when compared with the control, when 1 ㎍ / ml of extract or 1, 3 and 10 ㎍ / , The extracts of Puerariae Radix, and the extracts of Ginkgo biloba extract, the ALP activity was increased by about 40% or more when the extract was administered alone, 5). Therefore, it was confirmed that a mixture of the mixture of the extract and the ginseng extract, especially at a ratio of 1:10, promoted the differentiation of periodontal ligament cells.

< Experimental Example  5> Confirmation of Root Root and Periodontal Growth Promoting Effect of Mixture of Extracts and Extracts

In order to confirm the effect of accelerating the root and periodontal growth of the mixture of ginseng extract and ginseng extract, an experiment was conducted in which the gums of the ginseng extract, ginseng extract or ginseng extract and ginseng extract were treated as follows .

Specifically, 1 μl of 5 mg / ml L. extract (1 μl added to 5 μg / ml) and 1 μl of 15,50 mg / ml Shin extract (1 μl to make 15 μg / ml) were added to 1 ml of PBS, The mixture was adjusted to a ratio of 1: 3 or 1: Then, four agarose beads (Affi-Gel Blue Gel, # 153-7301, Bio-Rad Laboratories Inc.) were washed with PBS, and the mixture was applied so that the beak extract, The reaction was allowed to proceed for at least 60 minutes. The other four beads as controls were reacted only with PBS. Thereafter, Molar was removed from the lower jaws at the sacrifice of C57BL / 6 mouse (BioLink) at 5 days of age and the size of the extracted tooth was compared with that of the Lilium extract, Shin extract or Lilium extract and Shin extract And embedded in agarose beads containing the mixture. As a control, the size of extracted teeth was embedded in agarose beads containing PBS. Agarose beads were grafted into capsules of 8-week-old C57BL / 6 host mouse kidneys, and the host mice were raised at 12 hours of light-dark cycle, room temperature, free-feeding conditions, sacrificed three weeks after transplantation, Respectively. The 3D image of the tooth was photographed by micro CT.

As a result, as shown in FIG. 6, growth of dental root and bone was hardly observed in the control group (PBS), and growth of root and periodontal tissues was minimal when the extract of Leucaceae or Shin extract was used alone. However, When ginseng extract was treated with a mixture of extracts, periodontal tissue growth was increased. At a ratio of 1: 10 compared with the 1: 3 ratio, root formation and alveolar bone were newly formed around the root growth and implanted teeth. Therefore, when the two extracts were mixed with each other, the effect of promoting root formation and periodontal tissue formation was better than that of the extract of L. extract or Shin.i extract alone. In particular, when the mixture ratio of L. extract: Shin extract was 1:10, It was confirmed that the promoting effect was the most excellent.

Hereinafter, a preparation example for a pharmaceutical composition, a quasi-drug composition, and a cosmetic composition containing as an active ingredient a combination of an extract of Lilac and a extract of Shin extract of the present invention is illustrated.

< Manufacturing example  1> Preparation of pharmaceutical composition

<1-1> Preparation of Tablets (Direct Pressurization)

5.0 mg of active ingredient

Lactose 14.1 mg

Crospovidone USNF 0.8 mg

0.1 mg of magnesium stearate

According to the conventional preparation method of tablets, the above ingredients were added in the prescribed amounts, uniformly mixed and pressed to prepare tablets.

<1-2> Preparation of tablets (wet assembly)

5.0 mg of active ingredient

Lactose 16.0 mg

Starch 4.0 mg

Presorbate 80 0.3 mg

2.7 mg of silicon dioxide

2.0 mg of magnesium stearate

The active ingredient was sieved and then lactose and starch were mixed. Then, the polysorbate was dissolved in pure water, and an appropriate amount was added to the active ingredient, lactose and starch mixture, followed by atomization. After drying, the granulate was sieved and mixed with colloidal silicon dioxide and magnesium starate. The granules were pressurized to prepare tablets.

< Manufacturing example  2> Preparation of quasi-drug composition

<2-1> Oral use Gaggle  Produce

0.1 to 1.0 g of the active ingredient

Xylitol 5 to 10 g

Ethyl alcohol 5-15 g

Sorbitol 5 to 15 g

10 to 100 mg of saccharin sodium

Sodium monofluorophosphate 500 to 1000 mg

Sodium lauryl sulfate 100-200 mg

Polysorbate 20 100 to 1000 mg

Pepaminite flavor 10 to 100 mg

Sodium benzoate 10-100 g

<2-2> Preparation of Toothpaste

Active ingredient 1.9 parts by weight

10.0 parts by weight of anhydrous silica

Xanthan gum 0.7 part by weight

Magnesium sulfate 2.0 parts by weight

Sodium monofluorophosphate 0.7 part by weight

Sorbitol 59.0 parts by weight

Polyethylene glycol 4.0 parts by weight

0.2 part by weight of sodium saccharin

Sodium lauryl sulfate 1.0 part by weight

Fragrance 0.5 part by weight

Purified water 20.0 parts by weight

<2-3> Preparation of liquid toothpaste

2.2 parts by weight of active ingredient

Sodium monofluorophosphate 0.7 part by weight

Sorbitol 54.0 parts by weight

Concentrated glycerin 2.0 parts by weight

Ethanol 5.0 parts by weight

Magnesium sulfate 2.0 parts by weight

0.2 part by weight of sodium saccharin

Carrageenan 0.5 part by weight

Sodium lauryl sulfate 0.5 part by weight

Polyglycerin fatty acid ester 2.0 parts by weight

0.9 parts by weight fragrance

Purified water 30 parts by weight

Claims (9)

The present invention relates to a pharmaceutical composition for promoting growth of root or periodontal tissue comprising a mixture of an extract of Lilac and a ginseng extract as an active ingredient, or for the prevention or treatment of periodontitis.
2. The composition of claim 1, wherein the mixture is a mixture of a rosemary extract and a ginseng extract in a mass ratio of 1: 1 to 1:50.
The composition of claim 1, wherein the mixture inhibits nitric oxide (NO) activity and tartrate-resistant acid phosphatase (TRAP) activity and increases ALP (alkaline phosphatase) activity.
A composition for controlling the growth of root or periodontal tissue comprising a mixture of an extract of Lilac and a ginseng extract as an active ingredient, or a composition for preventing or improving periodontal disease.
5. The quasi-drug composition according to claim 4, wherein the quasi-drug composition is a dentifrice, an oral cleanser or an oral cleanser, for promoting growth of root or periodontal tissue, or for preventing or improving periodontitis.
5. A composition according to claim 4, wherein the mixture is a mixture of a rosemary extract and a ginseng extract in a mass ratio of 1: 1 to 1:50.
5. The composition of claim 4, wherein the mixture inhibits nitric oxide (NO) activity, inhibits TRAP (Tartrate-resistant acid phosphatase) activity, and increases ALP (alkaline phosphatase) activity.
The present invention relates to a health functional food for accelerating the growth of root or periodontal tissue or improving periodontal disease, which comprises a mixture of an ascorbic acid extract and a ginseng extract as an active ingredient.
The present invention relates to a cosmetic composition for promoting the growth of root or periodontal tissue or for the improvement of periodontitis, which contains as an active ingredient a mixture of an extract of Lilac and a ginseng extract.

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