CN113318051B

CN113318051B - Soothing and moisturizing extracting solution containing magnolia bark extract and preparation method thereof

Info

Publication number: CN113318051B
Application number: CN202110702414.9A
Authority: CN
Inventors: 高宏旗; 臧福坤; 张金艳; 曹义苗; 李广涛
Original assignee: Shanghai Linqingxuan Biotechnology Co ltd
Current assignee: Shanghai Linqingxuan Biotechnology Co ltd
Priority date: 2021-06-23
Filing date: 2021-06-23
Publication date: 2023-04-07
Anticipated expiration: 2041-06-23
Also published as: CN113318051A

Abstract

The invention discloses a soothing and moisturizing extracting solution containing magnolia bark extract and a preparation method thereof; the soothing and moisturizing extracting solution comprises a dendrobe extract, a mangnolia officinalis extract, a gentian extract, water and polyhydric alcohol, wherein the weight ratio of the dendrobe extract to the mangnolia officinalis extract to the gentian extract is 0.2-3:1: 0.8-12. The compound of the invention introduces the dendrobium extract to obviously improve the illumination stability of the magnolia bark extract and solve the problem of overall compatibility and cooperativity of the formula, thereby ensuring the stability and efficacy of the product formula, and the introduction of the magnolia bark extract to synergistically improve the anti-allergy efficacy of the gentian extract, and the compound of the dendrobium extract and the gentian extract improves the anti-light damage effect.

Description

Soothing and moisturizing extracting solution containing magnolia bark extract and preparation method thereof

Technical Field

The invention relates to the field of cosmetics, in particular to a soothing and moisturizing extracting solution containing a magnolia officinalis extract and a preparation method thereof.

Background

The radix Gentianae is dried root and rhizome of Gentiana striolata manshuric Kitag, gentiana scabra Bge, gentiana trifoliata Trichura pall or Gentiana rigescens Franch. Has effects in clearing away heat, eliminating dampness, and purging pathogenic fire from liver and gallbladder. Gentian extract has recently gained popularity as an emerging anti-allergy and soothing ingredient in the market, as well as a recognized functional effect. The improvement of the compatibility of gentian extract has also become a research focus, for example, in patent CN11044893A, the effect is improved by the compatibility with radix stephaniae tetrandrae.

Cortex Magnolia officinalis, also known as cortex Magnolia officinalis, and cortex Magnolia officinalis, dried bark from Magnolia officinalis of Magnolia genus of Magnoliaceae family, such as Magnolia officinalis of Magnolia officinalis Rehd. Has effects of activating qi-flowing, eliminating dampness, lowering adverse qi, and relieving asthma. The application of the magnolia officinalis in the personal care product is mainly focused on the aspects of antibiosis, acne removal, aging resistance, whitening and the like.

According to the invention, the combination of the radix gentianae extract and the magnolia officinalis extract has the effect of remarkably improving the anti-allergy effect of the radix gentianae extract, the combination of the radix gentianae extract and the magnolia officinalis extract has a synergistic interaction effect, and the magnolia officinalis extract can be used as a function enhancer for anti-allergy and relieving of the radix gentianae extract.

However, in the compounding process of the formula of the radix gentianae and mangnolia officinalis extract, the light stability of the formula is poor due to the introduction of the mangnolia officinalis extract, so that precipitates appear in the radix gentianae and mangnolia officinalis compound under the condition of natural light for 10-15 days, the anti-allergy effect of the compound is weakened, and the light stability of the mangnolia officinalis extract is a key for preventing the stability and the efficacy of the compound formula. The instability of the magnolia bark extract to light is rarely reported at present. A few research reports suggest that it is not affected by lighting conditions. In the practical test process, the influence of the illumination condition in a short time is limited and has no obvious change under the liquid formula state, the illumination instability of the magnolia officinalis extract is gradually shown along with the prolonging of the illumination time, the formula solution is precipitated, and the phenolic hydroxyl is accelerated and oxidized under the illumination condition preliminarily presumed to cause the precipitation of certain components.

According to the reports of the existing documents, the two main active ingredients in the magnolia officinalis, namely magnolol and honokiol, contain phenolic hydroxyl groups in the structure, have poor stability and are easy to oxidize. And the water solubility is poor, so that the development and the application of the magnolia officinalis are limited. In the prior art, the related problems of solubility and instability of the magnolia bark extract are solved by adopting structure modification, derivative preparation, micelle solubilization, nanotechnology, cyclodextrin and liposome coating technologies, and the applicability and bioavailability of the magnolia bark extract are improved. However, the prior art has high cost, complex preparation process and easy generation of byproducts in the process, and is not favorable for the development of green environmental protection.

Compared with the existing solution, the invention discovers that the dendrobe pectin polysaccharide modified according to certain conditions has better stabilizing effect on the extract of magnolia officinalis. The two are compounded to solve the problem of instability of illumination of the magnolia officinalis extract. The modified dendrobe pectin polysaccharide can be used as a stabilizer of a magnolia officinalis extract, so that the whole product formula has good efficacy stability.

Disclosure of Invention

In view of the above defects of the prior art, the technical problem to be solved by the invention is how to solve the problem of unstable illumination of magnolia officinalis extract in a formula through formula combination, thereby obtaining a soothing and moisturizing extracting solution containing magnolia officinalis extract with high stability.

In order to achieve the purpose, the invention provides a soothing and moisturizing extracting solution containing a magnolia officinalis extract, which comprises a dendrobe extract, a magnolia officinalis extract, a gentian extract, water and polyhydric alcohol.

As one embodiment of the invention, the weight ratio of the dendrobium extract, the magnolia bark extract and the gentian extract is 0.2-3:1: 0.8-12. The proportion of the extract is determined by comprehensively considering the efficacy, the stability and the cost. In some embodiments, the weight ratio of the dendrobium extract, magnolia bark extract and gentian extract is 1:3.

As an embodiment of the present invention, the polyhydric alcohol is one selected from butylene glycol, propylene glycol and dipropylene glycol. The concentration of the polyhydric alcohol in the soothing and moisturizing extracting solution is 30-55%.

As one embodiment of the invention, the dendrobium extract is compounded by fresh dendrobium extract and dry dendrobium extract. The weight ratio of the fresh dendrobium extract to the dry dendrobium extract is 2-8: 1.

In some embodiments, the weight ratio of the fresh dendrobium extract to the dry dendrobium extract is 4:1.

As an embodiment of the present invention, the dry dendrobium extract is an ultrasonically modified dry dendrobium extract.

The invention also provides a preparation method of the soothing and moisturizing extract containing the magnolia officinalis extract, which comprises the following steps:

s1, performing reflux extraction on mangnolia officinalis medicinal decoction pieces by using ethanol, concentrating an extracting solution, adjusting pH, centrifuging, collecting precipitates, and drying to obtain a mangnolia officinalis extract;

s2, smashing the gentian medicinal materials, then carrying out water extraction, decoloring, filtering, and concentrating to dryness to obtain a gentian extract;

s3, homogenizing fresh dendrobium strips, carrying out water extraction, centrifuging, decoloring supernatant, filtering, concentrating filtrate, adding alcohol, standing, centrifuging, collecting precipitate, and drying to obtain a fresh dendrobium extract;

s4, crushing the dry dendrobium, then carrying out water extraction, centrifuging, decoloring supernatant, filtering, concentrating filtrate, adding alcohol, standing, centrifuging, collecting supernatant, adding alcohol into supernatant, standing, centrifuging, collecting precipitate, and drying to obtain a dry dendrobium extract;

s5, dissolving the magnolia bark extract and the gentian extract in polyhydric alcohol, dissolving the fresh dendrobe extract and the dry dendrobe extract in water, mixing, and filtering to obtain the soothing and moisturizing extracting solution.

As an embodiment of the invention, dissolving the fresh dendrobium extract and the dry dendrobium extract in water in the step S5 is to dissolve the dry dendrobium extract in water, adding the fresh dendrobium extract by 300-600W ultrasonic wave for 30-50min, and stirring at room temperature to obtain the dendrobium extract.

Further, in step S1, ethanol reflux extraction is carried out for 1-2 hours by using 70% ethanol in an amount which is 9-11 times that of the ethanol reflux extraction.

Further, in step S1, the solvent is recovered from the extract until the alcohol concentration is 10% or less, the pH is adjusted to 8.5 to 9.0 by adding sodium hydroxide, and the filtrate is filtered.

Further, in step S1, the pH of the filtrate is adjusted to 5.5-6.0 with hydrochloric acid, and then centrifugation is performed.

Further, in step S2, the water extraction is carried out by using 9-11 times of water and extracting for 0.8-1.2 hours at 75-85 ℃.

Further, in step S3, the water extraction is performed for 1-2 hours at 80 ℃ by using 10 times of water by weight.

Further, in step S3, the filtrate is concentrated to 1/8-1/5 of the original volume, ethanol is added to 70%, and the mixture is kept stand for 3-4 hours at the temperature of 2-8 ℃.

Further, in step S4, the water is extracted for 1-2 hours at 80 ℃ with 15 times of the weight of the water.

Further, in step S4, the filtrate is concentrated to 1/8-1/5 of the original volume, ethanol is added to 70%, and the mixture is kept stand for 3-4 hours at the temperature of 2-8 ℃.

Further, in step S4, ethanol is added to the supernatant to 80%, and the mixture is allowed to stand at 2-8 ℃ for 4 hours.

In some embodiments, the specific preparation of the soothing and moisturizing extract comprises the following steps:

(1) Reflux-extracting cortex Magnolia officinalis decoction pieces with 10 times of 70% ethanol for 1-2 hr, recovering solvent from the extractive solution until the concentration of ethanol is below 10%, adjusting pH to 8.5-9.0 with sodium hydroxide, filtering, adjusting pH of the filtrate to 5.5-6.0 with hydrochloric acid, centrifuging, collecting precipitate, drying to obtain cortex Magnolia officinalis extract,

(2) Pulverizing radix Gentianae, extracting with 10 times of water at 80 deg.C for 1 hr, decolorizing the filtrate with active carbon, filtering, concentrating to dry to obtain radix Gentianae extract,

(3) Homogenizing fresh herba Dendrobii strips, adding 10 times of water, extracting at 80 deg.C for 1-2 hr, centrifuging, decolorizing the supernatant with activated carbon, filtering, concentrating the filtrate to 1/8-1/5 of the original volume, adding ethanol to 70%, standing at 2-8 deg.C for 3-4 hr, centrifuging, collecting precipitate, drying to obtain herba Dendrobii extract A,

(4) Pulverizing dried herba Dendrobii, adding 15 times of water, extracting at 80 deg.C for 1-2 hr, centrifuging, decolorizing the supernatant with active carbon, filtering, concentrating the filtrate to 1/8-1/5 of the original volume, adding ethanol to 50%, standing at 2-8 deg.C for 2-4 hr, centrifuging, collecting supernatant, adding ethanol to 80% of the supernatant, standing at 2-8 deg.C for 4 hr, centrifuging, collecting precipitate, drying to obtain herba Dendrobii extract B,

(5) Dissolving the dendrobium extract B in the step (4) in water, performing ultrasonic treatment at 600W for 30min, then adding the dendrobium extract A in the step (3), stirring at room temperature for 20 min to obtain a dendrobium extract, wherein the weight ratio of the dendrobium extract B to the dendrobium extract A is 1: 2-8,

(6) And (3) dissolving the magnolia officinalis extract in the step (1) and the gentiana scabra bunge extract in the step (2) in polyhydric alcohol, mixing with the dendrobium extract in the step (5), and filtering to obtain the soothing and moisturizing extracting solution containing the magnolia officinalis extract.

The invention also provides application of the soothing and moisturizing extract containing the magnolia bark extract in skin care products, cosmetics, washing and caring products and oral care products.

The plant polysaccharide has special physicochemical properties and a polyhydroxy structure, is easy to form intramolecular and intermolecular hydrogen bonds, and particularly has certain interaction with phenolic compounds. This interaction is related to the origin, composition, molecular structure, molecular weight of the polysaccharide, and the structural properties of the phenolic compound. The polysaccharide and the phenolic substance can be combined with each other through various forces, such as hydrophobic interaction, hydrogen bond, ionic bond and the like, and the interaction of the two has important practical significance.

The skeleton of the dendrobium cell wall is a three-dimensional matrix composed of polysaccharide macromolecules, wherein the structure comprises cellulose, hemicellulose, pectin and the like. Different polysaccharides have different interaction with mangnolia officinalis polyphenol, and a scheme for stabilizing the mangnolia officinalis extract can be found through interaction of dendrobium polysaccharides with mangnolia officinalis phenolic substances. Degradation is one of important methods for carrying out structural modification on polysaccharide, and mainly adopts physical, chemical and biological methods, ultrasonic or enzymolysis treatment promotes the composition and structural modification of polysaccharide, the molecular chain of dendrobe pectin is broken, the pectin chain is shortened, but the pyranose configuration of pectin is not changed, namely, the radius is reduced in a gradient manner, the viscosity is reduced, the emulsifying property of pectin is favorably improved, and the emulsifying property and the emulsifying stability of the pectin can be improved.

It should be noted that the modified dendrobe polysaccharide in combination with the unmodified polysaccharide can improve the light stability of the magnolia bark extract through thickening, antioxidation, emulsification and other effects. The stability effect of the fresh dendrobium and the dry dendrobium compound to the magnolia officinalis extract is better than that of the fresh dendrobium or the dry dendrobium which is singly used. This is likely to be strongly correlated with the polysaccharide variability. The polysaccharide content of the fresh dendrobium officinale is higher than that of the dried dendrobium officinale, the polysaccharide compositions of the fresh dendrobium officinale and the dried dendrobium officinale are obviously different, the arrangement of the molecular weight of the fresh dendrobium officinale is uniform, only one molecular weight fragment exists, and the molecular weight is 1572KDa. The molecular weight fragments in the dried dendrobium stem are distributed and concentrated in 3 molecular weight segments, the molecular weight is 1527KDa, 583KDa and 8KDa respectively, and the antioxidant activity of the fresh dendrobium stem is better than that of the dried dendrobium stem.

Therefore, in order to solve the problem that the magnolia bark extract is unstable after long-term illumination, the unmodified fresh dendrobium extract A adopted by the invention has higher viscosity, plays a certain thickening role when being applied to a product, and has better antioxidation; the ultrasonically modified dendrobium extract B polysaccharide emulsion has good emulsification property, so that a certain emulsification effect can be exerted; the micromolecule polysaccharide in the dendrobium extract B can be bridged between macromolecule sugar chains to form a compact reticular gel system, so that the interaction of the dendrobium extract B on the magnolia officinalis extract is enhanced; the magnolia bark extract has good formula stability, thereby solving the problem of instability of the magnolia bark extract.

Compared with the prior art, the invention has the following beneficial effects:

1) The newly introduced dendrobium component can be used as a cosmetic raw material with the moisturizing and skin care functions, so that the original formula has the moisturizing performance, and the formula is more natural and is more easily accepted by the market.

2) More importantly, the dendrobium extract is introduced in the compounding process, so that the illumination stability of the magnolia extract is obviously improved, the problem of overall compatibility synergy of the formula is solved, and the stability and the efficacy of the product formula are guaranteed.

3) The combination of the radix gentianae extract with the anti-allergy and relieving functions of the invention introduces the magnolia officinalis extract to synergistically improve the anti-allergy effect of the radix gentianae extract, and the combination of the radix gentianae extract and the magnolia officinalis extract improves the anti-light injury effect.

Drawings

Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:

FIG. 1 is an external view of a preferred embodiment of the present invention during 1 month of illumination;

FIG. 2 is an appearance view of a preferred embodiment of the present invention in 2 months of light;

FIG. 3 is an appearance view of a preferred embodiment of the present invention in 3 months of light;

FIG. 4 is a graph showing the results of an anti-LPS stimulation test according to a preferred embodiment of the present invention;

FIG. 5 is a graph of the results of an antihistamine injury validation according to a preferred embodiment of the invention;

FIG. 6 is a graph showing the results of the evaluation experiment of the light damage resistance according to the preferred embodiment of the present invention.

Detailed Description

The present invention will be described in detail with reference to examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that it would be apparent to those skilled in the art that several modifications and improvements can be made without departing from the inventive concept. All falling within the scope of the present invention.

Dendrobium nobile of the following examples is Dendrobium officinale Kimura et Migo, an orchid plant produced in Tianmu mountain of Zhejiang.

Example 1

100g of officinal magnolia decoction pieces are extracted by 10 times of 70 percent ethanol under reflux for 1 hour. Recovering solvent from the extractive solution to alcohol concentration of 8%. Adjusting pH to 8.5-9.0 with 10% sodium hydroxide solution. Filtering, and adjusting pH of the filtrate to 5.5-6.0 with 10% hydrochloric acid. Centrifuging and collecting the precipitate. Drying to obtain cortex Magnolia officinalis extract 7.9g.

Pulverizing 125g of radix Gentianae, and extracting with 10 times of water at 80 deg.C for 1 hr. Adding active carbon into the filtrate for decolorization. Filtering, and concentrating to dryness to obtain radix Gentianae extract 18.3g.

Fresh dendrobe strips (150 g) were homogenized. 1.5kg of water was added. Extracting at 80 deg.C for 1 hr. And (4) centrifuging. Adding active carbon into the supernatant for decolorization. Filtering, and concentrating the filtrate to 1/5 of the original volume. Adding ethanol to 70%, and standing at 2-8 deg.C for 4 hr. Centrifuging and collecting the precipitate. Drying to obtain herba Dendrobii extract A6.0g. 50g of dried dendrobium is crushed and then 750g of water is added. Extracting at 80 deg.C for 1 hr. And (4) centrifuging. Adding active carbon into the supernatant for decolorization. Filtering, and concentrating the filtrate to 1/8 of the original volume. Adding ethanol to 50%, and standing at 2-8 deg.C for 4 hr. Centrifuging and collecting supernatant. Adding ethanol into the supernatant to 80%, and standing at 2-8 deg.C for 4 hr. Centrifuging, collecting precipitate, and drying to obtain herba Dendrobii extract B3.1g.

Taking 1g of herba Dendrobii extract B, and dissolving in 250g of water. And (3) after ultrasonic treatment is carried out at 600W for 30 minutes, adding 4g of the dendrobium extract A, and uniformly stirring to obtain the dendrobium extract. Preparing a solution from 5g of magnolia officinalis extract, 15g of gentian extract and 225g of butanediol, mixing with the dendrobium extract, uniformly stirring, and filtering to obtain the soothing and moisturizing extract containing the magnolia officinalis extract.

Example 2

Example 2 differs from example 1 in that the weight ratio of the dendrobe extract, magnolia bark extract and gentian extract is 0.2: 1.

Example 3

Example 3 differs from example 1 in that the weight ratio of the dendrobium extract, magnolia bark extract and gentian extract is 2: 1: 5.

Example 4

The difference between the embodiment 4 and the embodiment 1 is that the weight ratio of the dendrobium extract B to the dendrobium extract A in the prepared dendrobium extract is 1: 2.

Comparative example 1

Comparative example 1 differs from example 1 in that comparative example 1 does not contain dendrobe extract.

Comparative example 2

Comparative example 2 differs from example 1 in that the dendrobe extract B in comparative example 2 was not ultrasonically modified.

Comparative example 3

Comparative example 3 differs from example 1 in that the dendrobium extract of comparative example 3 is only dendrobium extract a (fresh dendrobium extract) and does not comprise dendrobium extract B (dry dendrobium extract).

Comparative example 4

Comparative example 4 differs from example 1 in that the dendrobe extract of comparative example 4 is only the ultrasonically modified dendrobe extract B (dry dendrobe extract) and does not comprise dendrobe extract a (fresh dendrobe extract).

Comparative example 5

Comparative example 5 differs from example 1 in that comparative example 5 does not contain magnolia bark extract.

Experiment of stability of illumination

The general stability includes several stability conditions of high and low temperature, acid-base pH, oxygen, and light depending on the application and storage conditions of the daily chemical product. Wherein the light stability is an important aspect of the product quality stability. The test method comprises the following steps: the sample to be measured is separately packed in a transparent glass bottle and placed on an experiment table with sufficient illumination conditions, the color, the pH value and the change of the appearance along with the time of the sample are observed, and the appearance result is shown in figures 1-3. And (3) judging: whether each observation index of the 3-month illumination test is in a preset index range or not is shown in tables 1-3.

TABLE 1 appearance change under light for different samples

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TABLE 2 variation of the chromaticity Pt-Co values of different samples under different illumination times

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The adopted colorimeter has the model of

PFxi series 195/1 model high precision spectrophotometric colorimeter designed for colorimetric analysis of light transmissive samples such as liquid chemicals, fuel oil, beer, water, and the like. The measuring range is 0-500mg Pt/l. The baseline 0mg Pt/l is the color value of deionized water. Values between 0 and 500mg Pt/l indicate that the sample is clear and haze free. The reason why the Pt/l exceeds 500mg is that the suspended matter causes light scattering and lowers the light transmittanceTherefore, the color of the sample will be greater than 500mg Pt/l.

TABLE 3 pH Change for different samples

Name (R)	0 week	1 week	2 weeks	3 weeks	4 weeks	8 weeks	For 10 weeks	For 12 weeks
									Example 1	5.31	5.26	5.64	5.22	5.19	5.47	5.43	5.40
Example 2	5.30	5.20	5.41	4.91	5.07	5.11	5.10	5.08
									Example 3	5.32	5.22	5.54	5.21	5.28	5.08	5.06	5.07
Example 4	5.31	5.21	5.22	4.73	4.99	4.83	4.93	4.92
									Comparative example 1	5.34	5.00	5.05	4.57	4.81	4.36	4.35	4.36
Comparative example 2	5.33	5.23	5.45	5.29	5.26	5.21	5.24	5.25
									Comparative example 3	5.35	5.27	5.48	5.13	5.29	5.08	5.09	5.07
Comparative example 4	5.32	5.27	5.42	5.34	5.41	5.21	5.23	5.22
									Comparative example 5	5.34	5.45	5.45	5.57	5.52	5.18	5.17	5.18

As can be seen from Table 3, the pH values of the examples and comparative examples did not change significantly during the 3-month period of light irradiation.

Efficacy verification test

Test 1 in vitro anti-LPS stimulation test of mononuclear macrophages

Lipopolysaccharide (LPS) is a component of the outer wall of the cell wall of gram-negative bacteria. When the stress state or the immune function of serious trauma, infection and the like is reduced, a large amount of LPS can enter the circulation of a human body, stimulate a monocyte-macrophage system and other immune cells to generate and release various inflammatory mediators, and enable the inflammatory reaction to be out of control. LPS can make RAW264.7 cell produce violent reaction, secrete a plurality of inflammation-related mediators and cytokines. Among them, nitric Oxide (NO) is not only involved in the development of inflammatory reaction, but also released in an amount representing the degree of activity of cells to some extent.

The anti-allergy and soothing effects of the sample of example 1 were evaluated using Nitric Oxide (NO) secretion from the monocyte macrophage cell line RAW264.7 as an index.

Cell: raw264.7; stimulus: LPS (1. Mu.g/ml); the instrument comprises the following steps: an enzyme-labeling instrument; and (3) detection: an inflammatory factor.

As shown in FIG. 4, the soothing and moisturizing extract containing Magnolia bark extract in example 1 was effective in inhibiting the generation of NO induced by LPS, and has anti-allergy and soothing effects.

Test 2 clinical verification of antihistaminic Damage

Histamine is a mediator released from mast cells and causes dilation of blood capillaries and increased permeability, spasm of smooth muscle, increased secretory activity of glands, etc., resulting in local congestion, edema, increased secretion, and contraction of smooth muscle of bronchial and digestive tracts. The clinical manifestations of the allergic reaction are type I allergic reactions, which can cause symptoms of pruritus, erythema, wheeze, rhinorrhea, sneeze, asthma, labored breathing, itching eyes, conjunctival congestion, and the like, and the symptoms are common allergic symptoms.

Therefore, the inhibition effect on histamine stimulation in clinic can be used as an effective method for evaluating the antiallergic drugs. Position: the inner side of the arm; stimulus: histamine hydrochloride; sample preparation: example 1; the instrument comprises: VISIA; and (4) judging a result: test methods of the VS control group of the test object group: the tested part is evenly smeared with histamine hydrochloride of 5 percent, the tested substance and the substrate contrast of different concentrations are smeared after 5min, and the pictures are observed and taken at different times. The different concentrations of the test substances were: 1%,2% and 5%. The photographing time was observed to be 20 minutes after painting. As shown in fig. 5, the subject accelerated the repair of histamine-induced damage and was dose-dependent.

Test 3 evaluation test of light damage resistance

Position: the inner side of the arm; stimulus: UVB (3900 mJ/cm) ² ) (ii) a Sample 1: example 1; sample 2: comparative example 5; the instrument comprises the following steps: VISIA; and (5) judging a result: test methods of the VS control group of the test object group: the test substance and the substrate control with different concentrations are smeared firstly, and then are irradiated by UVB for 80 minutes, and the pictures are observed and photographed. The different concentrations of the test substances were: 0.5%,1% and 2%. As shown in fig. 6, the subject slowed UVB skin damage and presented a dose-dependent profile.

The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.

Claims

1. A soothing and moisturizing extracting solution containing a magnolia bark extract is characterized by comprising a dendrobe extract, a magnolia bark extract, a gentian extract, water and polyhydric alcohol; the dendrobium extract is compounded by a fresh dendrobium extract and a dry dendrobium extract, wherein the weight ratio of the fresh dendrobium extract to the dry dendrobium extract is (2) - (8); the dry dendrobium extract is an ultrasonically modified dry dendrobium extract; the weight ratio of the dendrobium extract to the magnolia bark extract to the gentian extract is (0.2-3); the polyhydric alcohol is selected from one of butanediol, propanediol and dipropylene glycol; the concentration of the polyhydric alcohol in the soothing and moisturizing extracting solution is 30-55%;

the soothing and moisturizing extracting solution is prepared by a method comprising the following steps:

s1, performing reflux extraction on mangnolia officinalis medicinal decoction pieces by using ethanol, concentrating an extracting solution, adjusting pH, centrifuging, collecting precipitate, and drying to obtain a mangnolia officinalis extract;

s5, dissolving the magnolia bark extract and the gentian extract in polyhydric alcohol, dissolving the fresh dendrobe extract and the dry dendrobe extract in water, mixing, and filtering to obtain the relieving and moisturizing extracting solution; wherein, dissolving the fresh dendrobium extract and the dry dendrobium extract in water is dissolving the dry dendrobium extract in water, carrying out ultrasonic treatment at 300-600W for 30-50min, adding the fresh dendrobium extract, and stirring at room temperature to obtain the dendrobium extract.

2. The soothing and moisturizing extract of claim 1, wherein the weight ratio of the dendrobium extract, the magnolia bark extract and the gentian extract is 1.

3. The soothing and moisturizing extract of claim 1, wherein the weight ratio of the fresh dendrobe extract to the dry dendrobe extract is 4.

4. Use of the soothing moisturizing extract of claim 1 in the preparation of a cosmetic.