CN106924120A - A kind of oral care substance of antibacterial, fresh breath - Google Patents
A kind of oral care substance of antibacterial, fresh breath Download PDFInfo
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- CN106924120A CN106924120A CN201710275145.6A CN201710275145A CN106924120A CN 106924120 A CN106924120 A CN 106924120A CN 201710275145 A CN201710275145 A CN 201710275145A CN 106924120 A CN106924120 A CN 106924120A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
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Abstract
The invention provides a kind of antibacterial, the oral care substance of fresh breath, comprising the bark of official magnolia bark extract and fruit-vegetable plant tunning；The bark of official magnolia bark extract includes Magnolol and Honokiol；Polypeptide, amino acid that the fruit-vegetable plant tunning is produced comprising superoxide dismutase, protease, lipase, cellulase and fermentation.It is applied in combination by by the bark of official magnolia bark extract and fruit-vegetable plant tunning both natural components, play significant synergistic function, suppress attachment and growth of the bacterium in tooth and oral cavity, suppress the deposition of protein, starch and fat, fundamentally solve " implication " this oral problem.And the oral care substance that the present invention is provided can be made various oral preparations by adding auxiliary material, not only with good stability and using effect, but also greatly enrich the type of service of oral care substance.
Description
Technical field
The present invention relates to oral care product technical field, more particularly to a kind of antibacterial, fresh breath oral care substance.
Background technology
" implication " is mainly the volatile sulfur as produced by the materials such as the corrupt protein decomposed in oral cavity of oral cavity bacterium
Caused by compound and the aliphatic acid with peculiar smell.For this oral problem, traditional oral care product has two major classes.The
One class oral care product reaches covering, removes the effect of malodoring gas by adding fragrant composition；Equations of The Second Kind oral cavity
Care product removes bacterium and swill by adding special rubbing agent using mechanical friction.Whether addition distributes perfume (or spice)
The composition of taste or special rubbing agent, traditional oral care product can only all take stopgap measures and can not effect a permanent cure.
The content of the invention
(1) technical problem to be solved
For traditional oral care product when implication is administered the problem that cannot effect a radical cure, the invention provides a kind of antibacterial,
The oral care substance of fresh breath, the oral care substance by by the bark of official magnolia bark extract and fruit-vegetable plant tunning both
Natural component is applied in combination, and plays significant synergistic function, can suppress attachment and taste of the bacterium in tooth and oral cavity
It is raw, the deposition of protein, starch and fat can be suppressed, " implication " this oral problem is fundamentally solved, so as to reach length
The pure and fresh effect of effect.
(2) technical scheme
In order to solve the above-mentioned technical problem, the invention provides following technical scheme：
A kind of oral care substance of antibacterial, fresh breath, the oral care substance includes the bark of official magnolia bark extract and fruits and vegetables
Plant fermentation product,；
The bark of official magnolia bark extract (is for example extracted purchased from the Magnolia bark of Draco Natural Products companies
Thing, trade name：Magnolia Liquid Extract, article No. CSFMU-TY-030, and increased using monoglyceride citrate
The molten scattered aqueous solution.) include Magnolol and Honokiol.
The fruit-vegetable plant tunning, comprising superoxide dismutase, protease, lipase, cellulase and fermentation
The polypeptide of generation, amino acid.
Preferably, the mass ratio of the bark of official magnolia bark extract and the fruit-vegetable plant tunning is 1：(1~100).
Preferably, the mass ratio of the bark of official magnolia bark extract and the fruit-vegetable plant tunning is 1：(1~20).
It is highly preferred that the mass ratio of the bark of official magnolia bark extract and the fruit-vegetable plant tunning is 1：(1~5).
Preferably, the oral care substance is also comprising the auxiliary material that can be received by oral cavity；
Preferably, it is auxiliary used by auxiliary material, preparation mouthwash used by the accessory package chewing gum containing preparation that can be received by oral cavity
Material, any one prepared auxiliary material used by toothpaste, prepare auxiliary material used by buccal tablets, prepare used by mouth sprays in auxiliary material.
Preferably, accessory package used by the preparation mouthwash contains solvent, NMF, buffer, surfactant, sweet taste
Agent, flavor enhancement and preservative；And/or
Accessory package used by the preparation mouth sprays is containing solvent, NMF, buffer, surfactant, preservative and sweet
Taste agent.
Preferably, when in oral care substance also comprising the auxiliary material that can be received by oral cavity, the bark of official magnolia bark extract exists
Content in the oral care substance is 0.01wt%~0.25wt%, and the fruit-vegetable plant tunning is in the MC
Content in thing is 0.01wt%~25wt%.
Preferably, the auxiliary material that can be received by oral cavity auxiliary material, the bark of official magnolia bark extract used by preparation mouthwash
Content in the oral care substance is 0.01wt%~1.25wt%, and the fruit-vegetable plant tunning is protected in the oral cavity
Content in reason thing is 0.01wt%~25wt%；And/or
, to prepare auxiliary material used by mouth sprays, the bark of official magnolia bark extract is described for the auxiliary material that can be received by oral cavity
Content in oral care substance is 0.01wt%~5wt%, and the fruit-vegetable plant tunning is in the oral care substance
Content is 0.01wt%~25wt%.
Preferably, the fruit-vegetable plant tunning is prepared as follows：
(1) fruits and vegetables are ground into fruits and vegetables slag, add cellulase and pectase to be digested, obtain digesting mixture；
(2) pH of regulation enzymolysis mixture is 3.5~4.0, and accesses Bacillus acidi lactici and fermented, and obtains tunning；
(3) vacuum freeze drying：Tunning is concentrated, vacuum freeze drying is then carried out, fruit-vegetable plant fermentation is obtained
Product.
It is further preferred that the condition of the enzymolysis is：PH is 5.0~6.0, and the solid-liquid ratio of fruits and vegetables slag is 1:(2~3),
Temperature is 50~55 DEG C, and enzymolysis time is 4~5h, and cellulose enzyme concentration is 1.0~1.5wt%, and pectase enzyme concentration is
0.5~1.0wt%；
The condition of the fermentation is：Fermentation temperature is 18~20 DEG C, 20~24h of fermentation time, and inoculum concentration is 0.15
~0.20%；And/or
The condition of the vacuum freeze drying is：Cryogenic temperature is -20~-15 DEG C, and vacuum is 10~20Pa, cold-trap temperature
Spend is -45~-40 DEG C.
It is further preferred that in the fruit-vegetable plant tunning, protease content is more than 2000U/g, superoxides discrimination
Change enzyme content more than 5000U/g.
(3) beneficial effect
Above-mentioned technical proposal of the invention has the following advantages that：
(1), the present invention makes by by the bark of official magnolia bark extract and both natural components combination of fruit-vegetable plant tunning
With, significant synergistic function is played, attachment and growth of the bacterium in tooth and oral cavity can be suppressed, albumen can be suppressed
The deposition of matter, starch and fat, fundamentally solves " implication " this oral problem, so as to reach long-acting pure and fresh effect.
(2), this oral care substance that the present invention is provided is not only able to treat " implication " this oral problem, and can also
The various oral inflammations such as treatment stomatitis, gingivitis, periodontitis.
(3), the present invention enters by the bark of official magnolia bark extract and fruit-vegetable plant tunning both active component consumptions
Row is limited, it is ensured that two kinds of combinations of substances can play significant synergistic function when using.
(4), the oral care substance that the present invention is provided can be made chewing gum, mouthwash, toothpaste, mouth by adding all kinds of auxiliary materials
Chamber lozenge and mouth sprays, not only with good stability and using effect, but also have greatly enriched oral cavity shield of the present invention
The type of service of thing is managed, is easy to consumer to select.
(5), by by the bark of official magnolia bark extract and fruit-vegetable plant tunning with prepare auxiliary material or preparation used by mouthwash
Auxiliary material used by mouth sprays is hybridly prepared into mouthwash or mouth sprays, and fungistatic effect has reached more than 98%, have " implication " this
Continuously using after 3 days, implication is just made moderate progress the consumer of one oral problem, and continuous using after 10 days, oral problem is thoroughly controlled
More.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with the embodiment of the present invention, to this hair
Bright technical scheme is clearly and completely described.Obviously, described embodiment is a part of embodiment of the invention, and
It is not all, of embodiment.Based on the embodiment in the present invention, those of ordinary skill in the art are not making creative work
On the premise of the every other embodiment that is obtained, belong to the scope of protection of the invention.
The invention provides a kind of antibacterial, the oral care substance of fresh breath, the oral care substance includes Magnolia bark
Extract and fruit-vegetable plant tunning；
The bark of official magnolia bark extract includes Magnolol and Honokiol；
The fruit-vegetable plant tunning is produced comprising superoxide dismutase, protease, lipase, cellulase and fermentation
Raw polypeptide, amino acid.
There is the Magnolol and Honokiol contained in the bark of official magnolia bark extract efficient broad-spectrum antibacterial to act on, and can suppress
The growth of undesired bacteria in oral cavity.Superoxide dismutase, protease, lipase and the fibre contained in fruit-vegetable plant tunning
The plain enzyme of dimension can suppress deposition of the mouth debris such as protein, starch and fat in oral cavity, the superoxides discrimination for especially containing
Change enzyme and polypeptide, wherein the superoxide dismutase for containing has very strong effect of scavenging radical, wherein the polypeptide tool for containing
There is natural bacteriostasis efficacy, attachment of the bacterium in dental surface can be suppressed.Two kinds of natural components combine performance significantly
Synergistic function, substantially increase antibacterial efficiency, suppress oral cavity in protein, starch, fat deposition and oral cavity fresh and cool
Degree.
Additionally, this oral care substance that the present invention is provided is not only able to treat " implication " this oral problem, but also
The various oral inflammations such as stomatitis, gingivitis, periodontitis can be treated.
Contrasted under same concentrations by with single component, the oral care substance that the present invention is provided is for variation hammer
Bacterium, Fusobacterium nucleatum, porphyromonas gingivalis these representative oral bacterias have more efficient bacteriostasis, can be effective
Suppress the precipitation of protein, starch and fat, acted on better clear implication.
Additionally, using effect of the consumption of the bark of official magnolia bark extract and fruit-vegetable plant tunning also to product of the present invention has
Significant impact.Inventor has found that, when only single component, the consumption of two kinds of single components is more, and using effect is more notable.
But when both are used in mixed way, two kinds of consumptions of material are not The more the better.Found by numerous studies, when Magnolia bark is carried
Thing and the fruit-vegetable plant tunning are taken according to 1：When the mass ratio of (1~100) is used in mixed way, synergy preferably, enters one
Step is preferably 1：(1~20), optimum quality ratio is 1：(1~5).When both are according to 1：When the mass ratio of (1~5) is used in mixed way,
Synergistic is the most notable, and bacteriostasis and suppression protein, starch and fat deposition effect are protruded the most, most aobvious so as to reach
The fresh breath effect of work.
The bark of official magnolia bark extract (is for example extracted purchased from the Magnolia bark of Draco Natural Products companies
Thing, trade name：Magnolia Liquid Extract, article No. CSFMU-TY-030, and increased using monoglyceride citrate
The molten scattered aqueous solution.) include Magnolol and Honokiol.
The fruit-vegetable plant tunning is prepared as follows：
(1) fruits and vegetables are ground into fruits and vegetables slag, add cellulase and pectase to be digested, obtain digesting mixture.
By enzymolysis, macromolecular substances are hydrolyzed to small-molecule substance, are more easy to be immediately used by the body.
Fruits and vegetables species include but is not limited to pomegranate fruit, Cranberry, apple, carrot, strawberry, Kiwi berry, celery, dragon fruit,
One or more in orange, "Hami" melon.
In certain embodiments, the condition of the enzymolysis is：PH is 5.0~6.0, and the solid-liquid ratio of fruits and vegetables slag is 1:(2~
3), temperature is 50~55 DEG C, and enzymolysis time is 4~5h, and cellulose enzyme concentration is 1.0~1.5wt%, pectase enzyme concentration
It is 0.5~1.0wt%.Digested under this condition, enzymatic hydrolyzation may be up to 70%, it is soluble in the enzymolysis mixture for obtaining
Solid content can reach 5%.
(2) pH of regulation enzymolysis mixture is 3.5~4.0, and accesses Bacillus acidi lactici and fermented, and obtains tunning.
In certain embodiments, the condition of fermentation is：Fermentation temperature is 18~20 DEG C, 20~24h of fermentation time, and is connect
The amount of kind is 0.15~0.20%.Lactobacillus casei quantity is 5x10 in obtained tunning in such a situa-tion8CFU/mL,
Proteinase activity is 1500U/g.
(3) vacuum freeze drying：Tunning is concentrated, vacuum freeze drying is then carried out, fruit-vegetable plant fermentation is obtained
Product.
In certain embodiments, the soluble solid content in tunning can be concentrated to first by concentration
30%, vacuum freeze drying is then carried out again.The condition of vacuum freeze drying is：Cryogenic temperature is -20~-15 DEG C, vacuum
It is 10~20Pa, condenser temperature is -45~-40 DEG C.Fruit-vegetable plant of the moisture less than 1% is obtained by vacuum freeze drying
Tunning.
Quality index using fruit-vegetable plant tunning obtained in the preparation method that the present invention is provided is as follows：
In the fruit-vegetable plant tunning, protease content exceedes more than 2000U/g, superoxide dismutase content
5000U/g.And, it is also uniform with color, with distinctive fruits and vegetables fragrance, and with fermenting aroma, loosely without the excellent of caking
Point.
This oral care substance that the present invention is provided can be made into several formulations, so as to realize mouth by various use approach
Nurse in chamber.Therefore, the oral care substance that the present invention is provided except the bark of official magnolia bark extract and fruit-vegetable plant tunning both
Outside active component, the auxiliary material that can be received by oral cavity can be also included.The bark of official magnolia bark extract, fruit-vegetable plant tunning are in auxiliary material
Under effect, various oral care products stable in properties can be formulated into.
In some embodiments of the invention, it is described can by oral cavity receive accessory package containing prepare chewing gum used by auxiliary material,
Auxiliary material used by mouthwash is prepared, auxiliary material used by toothpaste is prepared, is prepared auxiliary material used by buccal tablets, prepares auxiliary material used by mouth sprays
In any one.
By adding above-mentioned auxiliary material, can by the present invention provide oral care substance be prepared into chewing gum, mouthwash, toothpaste,
Buccal tablets and mouth sprays, have greatly enriched the type of service of oral care substance of the present invention, and consumer can be according to demands of individuals
The suitable product of selection.
Inventor has found, although when the bark of official magnolia bark extract and fruit-vegetable plant tunning mix with above-mentioned all kinds of auxiliary materials all
With good stability and using effect, but with prepare mouthwash used by auxiliary material, prepare mouth sprays used by auxiliary material or preparation
When auxiliary material used by toothpaste is hybridly prepared into mouthwash, mouth sprays or toothpaste, using effect is the most notable, and fungistatic effect reaches
More than 98%.Understood through multiple clinical trial, by the bark of official magnolia bark extract and fruit-vegetable plant tunning and preparation mouthwash institute
Mouthwash, mouth sprays or toothpaste are hybridly prepared into auxiliary material, auxiliary material used by preparation mouth sprays or preparation toothpaste, are had " implication "
Continuously using after 3 days, implication just makes moderate progress the consumer of this oral problem, and continuous using after 10 days, oral problem is thorough
Cure.
Auxiliary material used by chewing gum, auxiliary material, auxiliary material used by preparation buccal tablets used by preparation toothpaste is prepared to may be selected to prepare mouth
Customary adjuvant when fragrant sugar, toothpaste, buccal tablets, the present invention is numerous to list herein.Such as, the auxiliary material used by chewing gum is prepared
Comprising gum base material, plasticizer, sweetener, flavor enhancement etc..Prepare the accessory package used by toothpaste and contain solvent, thickener, table
The acceptable abrasive material of face activating agent, tooth, dentifrice, sweetener, flavor enhancement etc..
In some embodiments of the invention, it is described preparation mouthwash used by accessory package containing solvent, NMF, buffer,
Surfactant, sweetener, flavor enhancement and preservative.
In some embodiments of the invention, the solvent is water；The NMF is selected from glycerine or propane diols, moisturizing effect
It is really good, moreover it is possible to increase the viscosity of mouthwash, can also promote the bark of official magnolia bark extract dissolubility in a solvent；Live on the surface
Property agent be selected from anion, nonionic or zwitterionic surfactant in any one, play hydrotropy effect；Buffer selects pH
It is 7.0~7.5 PBS cushioning liquid, is not only able to be completely dissolved fruit-vegetable plant tunning, and mouthwash can also be adjusted
PH；Any one in sweetener selection D-sorbite, xylitol, honey element；The flavor enhancement selects essential oil；It is described anti-
Rotten agent is sodium chloride.
In some embodiments of the invention, accessory package used by the preparation mouth sprays contains solvent, NMF, buffering
Agent, surfactant, preservative and sweetener.
Similarly, the solvent is most preferably water, and the NMF is most preferably glycerine or propane diols；The buffer is most
Suitable is the PBS cushioning liquid that pH is 7.0~7.5, and the surfactant is selected from anion, nonionic or amphion table
Any one in the activating agent of face, the sweetener is preferably any one in D-sorbite, xylitol, honey element；The anti-corrosion
Agent is preferably sodium chloride.
In some embodiments of the invention, it is described when in oral care substance also comprising the auxiliary material that can be received by oral cavity
Content of the bark of official magnolia bark extract in the oral care substance is 0.01wt%~0.25wt%, and the fruit-vegetable plant fermentation is produced
Content of the thing in the oral care substance is 0.01wt%~25wt%.
In some embodiments of the invention, the auxiliary material that can be received by oral cavity auxiliary material, institute used by preparation mouthwash
Content of the bark of official magnolia bark extract in the oral care substance is stated for 0.01wt%~1.25wt%, the fruit-vegetable plant fermentation
Content of the product in the oral care substance is 0.01wt%~25wt%；And/or
, to prepare auxiliary material used by mouth sprays, the bark of official magnolia bark extract is described for the auxiliary material that can be received by oral cavity
Content in oral care substance is 0.01wt%~5wt%, and the fruit-vegetable plant tunning is in the oral care substance
Content is 0.01wt%~25wt%.
In some embodiments of the invention, when the auxiliary material that can be received by oral cavity is to prepare auxiliary material used by toothpaste,
Content of the bark of official magnolia bark extract in the oral care substance is 0.01wt%~1.25wt%, the fruit-vegetable plant hair
Content of the ferment product in the oral care substance is 0.01wt%~25wt%.
It is below the several embodiments for providing of the invention.
The bark of official magnolia bark extract used is purchased from Draco Natural Products companies (trade name in the present invention：
Magnolia Liquid Extract, article No. CSFMU-TY-030, and using the monoglyceride citrate scattered water of solubilising
Solution).
Embodiment 1
Fruit-vegetable plant tunning is prepared first, and preparation method comprises the following steps (preparation condition is shown in Table 1)：
(1) apple is ground into slag, adds cellulase and pectase to be digested, obtain digesting mixture；The enzyme
The condition of solution is：PH is 5.0, and the solid-liquid ratio of fruits and vegetables slag is 1:2, temperature is 50 DEG C, and enzymolysis time is 5h, and cellulose is enzyme-added
It is 1.5wt% to measure, and pectase enzyme concentration is 1.0wt%.
(2) pH of regulation enzymolysis mixture is 3.5~4.0, and accesses Bacillus acidi lactici and fermented, and obtains tunning；
The condition of the fermentation is：Fermentation temperature is 20 DEG C, fermentation time 20h, and inoculum concentration is 0.15%.In such a situa-tion
Lactobacillus casei quantity is 5x10 in obtained tunning8CFU/mL, proteinase activity is 1500U/g.
(3) vacuum freeze drying：Tunning is concentrated into soluble solid content and is concentrated to 30%, then carried out true
Vacuum freecing-dry, obtains fruit-vegetable plant tunning；The condition of vacuum freeze drying is：Cryogenic temperature is -15 DEG C, and vacuum is
10Pa, condenser temperature is -40 DEG C.Fruit-vegetable plant tunning of the moisture less than 1% is obtained by vacuum freeze drying.
The quality index of obtained fruit-vegetable plant tunning is as follows：
In the fruit-vegetable plant tunning, protease content is 3000U/g, and superoxide dismutase content is 6000U/
g.And, color is uniform, loosely without caking, with distinctive fruits and vegetables fragrance, and with fermenting aroma.
Then, the bark of official magnolia bark extract and fruit-vegetable plant tunning are weighed according to the consumption in table 2, then by two components
It is sufficiently mixed, obtains the oral care substance of antibacterial, fresh breath.
Embodiment 2 to embodiment 5 preparation method with embodiment 1, difference is shown in Tables 1 and 2.
The present invention is also prepared for two groups of only reference products comprising single component.Wherein, in first group of reference product containing only
There is the bark of official magnolia bark extract (being shown in Table 2 the 6th groups), comprise only fruit-vegetable plant tunning in second group of reference product and (be shown in Table 2 the 7th
Group).In addition, the present invention is also prepared for the 3rd group of reference product, Magnolol and Honokiol is not included in the 3rd group of reference product,
But, instead of both, not comprising fruit-vegetable plant tunning, but used by protease, lipase and fibre using Cetylpyridinium Chloride
The mixture of the plain weight/mass percentage composition such as the enzyme and amino acid composition of dimension.
The fruit-vegetable plant tunning preparation condition of table 1
The bark of official magnolia bark extract of table 2 and fruit-vegetable plant tunning scale
| The consumption of the bark of official magnolia bark extract | The consumption of fruit-vegetable plant tunning | |
| 1 | 1g | 1g |
| 2 | 1g | 10g |
| 3 | 1g | 20g |
| 4 | 1g | 5g |
| 5 | 1g | 100g |
| 6 | 1g | 0 |
| 7 | 0 | 100g |
In Tables 1 and 21 to 5 represents embodiment 1 to embodiment 5,6 to 7 respectively represents control group 1 and control group respectively
2。
Antibacterial test：
A：Fruit-vegetable plant tunning；B：The bark of official magnolia bark extract.
Experimental strain：
Take Streptococcus mutans (ATCC 25175), Fusobacterium nucleatum (ATCC10953), porphyromonas gingivalis (ATCC
33277).It is placed in 37 DEG C of cultures.
Test A, B respective minimal inhibitory concentration (MIC) first：
Experiment is divided into positive controls (the bacteria suspension culture medium containing 0.1% Chlorhexidine), the negative control group (bacterium containing PBS
Suspension culture medium), experimental group (containing various concentrations active matter bacteria suspension culture medium).Above each group is respectively provided with 3 parallel groups.
A is dissolved in the water, preparation obtains the A samples that concentration is 5.0wt%；B is dissolved in water and monoglyceride lemon
In lemon acid esters, preparation obtains the B samples that concentration is 2.0wt% (wherein the content of magnolol and honokiol is 8wt%).
It is the A samples of 5.0wt% by concentration, the B samples of 0.50wt% are dissolved in fluid nutrient medium by two-fold dilution, respectively A
Sample：5.0wt%, 2.5wt%, 1.25wt%, 0.62wt%, 0.3125wt%；
B samples：2.0wt%, 1.0wt%, 0.5wt%, 0.25wt%, 0.125wt%.It is 2 × 10 by concentration6CFU·mL-1
Bacteria suspension and each group active matter liquid 1: 1 each 100 μ L in proportion, are inoculated in aseptic 96 orifice plate respectively, make the end of bacteria suspension dense
Spend is 1 × 106CFU·mL-1, it is placed in 24~48h of culture under appropriate condition of culture.Take OD values at nutrient solution test 600nm.Such as
Fruit OD value≤0.05 is judged to MIC.Result is as shown in table 3.
Table 3
According to the result of MIC, test A when less than MIC (wherein, A concentration be 1.0%, B concentration for 0.5%) respectively, with
And the bacteriostasis of combination.It is experimental group than down ratio that negative control group OD is worth to reduce percentage.
Table 4
As can be seen that A, B combination are used alone than A or B and have more preferable bacteriostasis.A is applied in combination with B, effectively
Reduce its Mlc.
By the 3rd group of reference product C：Cetylpyridinium Chloride concentration is 0.04%, protease, lipase, cellulase and amino acid
Deng the mixture of weight/mass percentage composition composition, the total concentration of protease, lipase, cellulase and amino acid is 1.00%, is surveyed
Try its bacteriostasis.Its effect is as shown in table 5.
Table 5
As can be seen that under the conditions of concentration identical, the fungistatic effect of reference product C is much not as good as the present invention.
In addition, the present invention also provide a comparison fungistatic effect when A and B are combined according to different proportion, it is respectively A (1.0%)+B
(2.0%) and A (1.0%)+B (0.005%), the fungistatic effect with A (1.0%)+B (0.5%) is contrasted.By the knot of table 5
As can be seen that when both are used in conjunction with, ratio is excessive or too small cannot all have significant synergy for fruit.
Embodiment 1 and oral care substance obtained in embodiment 2 are mixed with auxiliary material used by preparation chewing gum, is prepared into anti-
Bacterium, the chewing gum of fresh breath.
The chewing gum component table of table 6
Oral care substance obtained in embodiment 3 is mixed with auxiliary material used by preparation mouthwash, antibacterial, fresh breath is prepared into
Mouthwash.
The mouthwash component list of table 7
| Composition | Content (wt%) |
| The bark of official magnolia bark extract | 0.01 |
| Fruit-vegetable plant tunning | 0.20 |
| Water | 67.00 |
| Glycerine | 14.49 |
| Propane diols | 5.30 |
| Xylitol | 7.20 |
| Laruyl alcohol sodium sulfovinate | 1.80 |
| Edible Peppermint essential oil | 0.20 |
| Sodium chloride | 3.50 |
| PBS buffers (pH7.0~7.5) | 0.30 |
By oral care substance obtained in embodiment 4 respectively with prepare mouth sprays used by auxiliary material mix, be prepared into antibacterial, clearly
The mouth sprays of new implication.
The mouth sprays component list of table 8
By embodiment 5 and first group of reference product and oral care substance obtained in second group of reference product respectively with prepare tooth
Auxiliary material mixing, is prepared into the toothpaste of antibacterial, fresh breath used by cream.
The toothpaste component table of table 9
Clinical trial：
Consumer tests are done using toothpaste obtained in embodiment 5, and first group of reference product and second group of reference product
Its actual effect is verified, the degree self-appraisal of (1) breath freshening is carried out after using preceding, first use 0 hour, 1 hour, 4 hours
Marking and the detection of (2) halitosis detector implication value.
The standard of self-appraisal marking is as follows：
1=implications are very pure and fresh；2=implications are more pure and fresh；The neither pure and fresh also free from extraneous odour of 3=implications；Halitosis that 4=is slight；5=
Severe halitosis.
Halitosis detector used is the portable volatile sulfur compounds implication analyzer of Halimiter produced in USA, directly
The ppb concentration values for reading sulfide VSCs in subject's implication are connect, is typically set to halitosis patient with more than 150ppb.
Subjects：With ppb more than 150 and self-appraisal index is more than 3 as entry standard, voluntarily ginseng is filtered out in Shanghai City
Plus the personnel totally 45 of this test.It is divided into three groups, every group of 15 people.Result is as follows：
The toothpaste of the table 10 number of subjects's percentage of self-appraisal marking index less than or equal to 2 before and after
| Sample | 0h before gargling | 1h after gargling | 4h after gargling |
| Embodiment 5 | 0 | 80 | 73.33 |
| First group of reference product | 0 | 50.67 | 28.00 |
| Second group of reference product | 0 | 53.33 | 33.33 |
The implication value (x ± s, ppb) detected with Halimiter before and after the toothpaste of table 11 is on probation
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
The present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those within the art that：It still may be used
Modified with to the technical scheme described in foregoing embodiments, or equivalent is carried out to which part technical characteristic；
And these modification or replace, do not make appropriate technical solution essence depart from various embodiments of the present invention technical scheme spirit and
Scope.
Claims (10)
1. the oral care substance of a kind of antibacterial, fresh breath, it is characterised in that the oral care substance includes Magnolia bark and extracts
Thing and fruit-vegetable plant tunning；
The bark of official magnolia bark extract includes Magnolol and Honokiol；
The fruit-vegetable plant tunning is produced comprising superoxide dismutase, protease, lipase, cellulase and fermentation
Polypeptide, amino acid.
2. oral care substance according to claim 1, it is characterised in that the bark of official magnolia bark extract and the fruits and vegetables are planted
The mass ratio of thing tunning is 1：(1~100).
3. oral care substance according to claim 2, it is characterised in that the bark of official magnolia bark extract and the fruits and vegetables are planted
The mass ratio of thing tunning is 1：(1~20).
4. oral care substance according to claim 2, it is characterised in that the bark of official magnolia bark extract and the fruits and vegetables are planted
The mass ratio of thing tunning is 1：(1~5).
5. oral care substance according to claim 1, it is characterised in that the oral care substance by oral cavity also comprising can be connect
The auxiliary material received.
6. oral care substance according to claim 5, it is characterised in that the accessory package that can be received by oral cavity is containing preparing
Auxiliary material, auxiliary material, auxiliary material, auxiliary material, preparation used by preparation buccal tablets used by preparation toothpaste used by preparation mouthwash used by chewing gum
Any one in auxiliary material used by mouth sprays.
7. oral care substance according to claim 6, it is characterised in that accessory package used by the preparation mouthwash is containing molten
Agent, NMF, buffer, surfactant, sweetener, flavor enhancement and preservative；And/or
Accessory package used by the preparation mouth sprays contains solvent, NMF, buffer, surfactant, preservative and sweetener.
8. the oral care substance according to any one of claim 5 to 7, it is characterised in that when also being included in oral care substance
Can by oral cavity receive auxiliary material when, content of the bark of official magnolia bark extract in the oral care substance be 0.01wt%~
0.25wt%, content of the fruit-vegetable plant tunning in the oral care substance is 0.01wt%~25wt%.
9. oral care substance according to claim 8, it is characterised in that the auxiliary material that can be received by oral cavity is for preparation is gargled
Auxiliary material used by saliva, content of the bark of official magnolia bark extract in the oral care substance is 0.01wt%~1.25wt%,
Content of the fruit-vegetable plant tunning in the oral care substance is 0.01wt%~25wt%.
10. oral care substance according to claim 8, it is characterised in that the auxiliary material that can be received by oral cavity is to prepare
Auxiliary material used by mouth sprays, content of the bark of official magnolia bark extract in the oral care substance is 0.01wt%~5wt%,
Content of the fruit-vegetable plant tunning in the oral care substance is 0.01wt%~25wt%.
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| CN107951743A (en) * | 2017-12-30 | 2018-04-24 | 广州润虹医药科技股份有限公司 | A kind of toothpaste containing chitosan and preparation method thereof |
| CN108066216A (en) * | 2017-12-27 | 2018-05-25 | 国东启 | A kind of antiallergic releive composition, include its cleanser and preparation method |
| CN108186384A (en) * | 2018-01-24 | 2018-06-22 | 深圳市芭格美生物科技有限公司 | A kind of mouthwash and preparation method thereof |
| CN109157424A (en) * | 2018-10-12 | 2019-01-08 | 赵孔发 | A kind of preparation method and applications of Japan pepper essential oil microcapsules |
| CN109771312A (en) * | 2017-11-15 | 2019-05-21 | 好维股份有限公司 | Synergistic antimicrobial composition |
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| CN114795997A (en) * | 2022-05-30 | 2022-07-29 | 有货(北京)信息技术有限公司 | NMN-containing amino acid toothpaste and preparation method thereof |
| CN114983879A (en) * | 2022-07-20 | 2022-09-02 | 成都中医药大学 | Magnolol mouth wash and preparation method thereof |
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| CN108186384A (en) * | 2018-01-24 | 2018-06-22 | 深圳市芭格美生物科技有限公司 | A kind of mouthwash and preparation method thereof |
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| CN113318051B (en) * | 2021-06-23 | 2023-04-07 | 上海林清轩生物科技有限公司 | Soothing and moisturizing extracting solution containing magnolia bark extract and preparation method thereof |
| CN113318051A (en) * | 2021-06-23 | 2021-08-31 | 上海林清轩生物科技有限公司 | Soothing and moisturizing extracting solution containing magnolia bark extract and preparation method thereof |
| CN114795997A (en) * | 2022-05-30 | 2022-07-29 | 有货(北京)信息技术有限公司 | NMN-containing amino acid toothpaste and preparation method thereof |
| CN114795997B (en) * | 2022-05-30 | 2023-08-01 | 有货(北京)信息技术有限公司 | NMN-containing amino acid toothpaste and preparation method thereof |
| CN114983879A (en) * | 2022-07-20 | 2022-09-02 | 成都中医药大学 | Magnolol mouth wash and preparation method thereof |
| CN114983879B (en) * | 2022-07-20 | 2023-12-01 | 成都中医药大学 | Magnolol mouthwash and preparation method thereof |
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Application publication date: 20170707 |