CN110384725B - A Chinese medicinal composition for regulating oral flora and preparation method of active extract thereof - Google Patents
A Chinese medicinal composition for regulating oral flora and preparation method of active extract thereof Download PDFInfo
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- CN110384725B CN110384725B CN201910879260.3A CN201910879260A CN110384725B CN 110384725 B CN110384725 B CN 110384725B CN 201910879260 A CN201910879260 A CN 201910879260A CN 110384725 B CN110384725 B CN 110384725B
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- chinese medicine
- traditional chinese
- oral
- flora
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- A—HUMAN NECESSITIES
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
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Abstract
The invention relates to the technical field of traditional Chinese medicine oral care, and particularly discloses a traditional Chinese medicine composition for adjusting oral flora and a preparation method of an active extract of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following components in parts by mass: 20-30 parts of elsholtzia, 20-25 parts of astragalus, 10-20 parts of chrysanthemum morifolium and 5-10 parts of honeysuckle. The invention selects the Jiangxiang Chinese mosla herb, Hangzhou chrysanthemum and honeysuckle which are medicinal and edible Chinese medicinal materials to be compatible with the astragalus root to be used as the traditional Chinese medicine composition for preventing and treating the peculiar smell of the oral cavity, treating the oral ulcer or relieving the periodontitis, and according to the dialectical theory of the etiology of the traditional Chinese medicine, the Jiangxiang Chinese mosla herb induces diuresis to alleviate edema, regulates the stomach, dissolves dampness and diminishes inflammation; astragalus root, radix astragali induces diuresis to alleviate edema, removes toxicity and promotes granulation; the chrysanthemum morifolium ramat has the effects of clearing away heat and toxic materials and resisting bacteria; honeysuckle flower, flos Lonicerae, with the effects of dispelling wind-heat, clearing and removing blood toxin, and eliminating pathogenic factors by aromatic penetration. The raw materials are compatible, and play a role in preventing and adjusting oral flora together. The traditional Chinese medicine composition or the traditional Chinese medicine compound can effectively prevent and treat oral diseases caused by oral bacterial imbalance or certain pathogenic bacteria, and has remarkable oral care effect.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine oral care, and particularly relates to a traditional Chinese medicine composition for adjusting oral flora and preparation of an active extract thereof.
Background
Many people feel that the mouth odor exists in the self or the other party, so that the two parties generate certain gap and distance in communication, great influence is generated on life and work, and the normal social interaction is obstructed. The microbes planted in the oral cavity coexist, compete and antagonize at different positions, are closely related to the health and diseases of the oral cavity of a host, in the oral cavity, the oral cavity can keep a healthy state only by keeping dynamic ecological balance among the microbes and between the microbes and the oral cavity of the host, if external microbes exist in the oral cavity or the proportion of individual microbes or a plurality of microbes inherent in the oral cavity microbial ecology is obviously increased to become dominant bacteria of the planted positions, the oral cavity micro-ecological system balance is broken, and dysbacteriosis can occur to cause oral diseases. The distribution and the total number of oral flora of patients with oral odor are obviously different from those of normal people, the number of pathogenic bacteria related to oral diseases is obviously higher than that of the normal people, pathogenic dominant bacteria decompose substances in the oral cavity to generate volatile sulfides and fatty acid, the volatile sulfides in abnormal breath are obviously increased, and the oral odor is generated.
Aiming at oral peculiar smell caused by oral microecological imbalance, the current solution is to solve dysbacteriosis. The oral cavity disease is treated, the generation of harmful gas in the oral cavity is eliminated, the dietary structure is adjusted, the hygienic habit is improved, the disease is treated by antibiotics, and the like, but measures such as using substances such as chewing gum and the like to enhance the oral hygiene and brushing teeth everyday, using oral cavity gargle and the like also have certain effects. However, antibiotics are limited in use and many types of mouthwashes are often single-acting or poorly effective. The existing oral care products containing traditional Chinese medicine components have the function of masking the peculiar smell of the oral cavity, do not have the function of adjusting oral microorganisms, or have a certain killing function on oral harmful bacteria, but cannot provide the function of refreshing the oral cavity.
Oral ulcer is a common oral disease, and is easy to be in a state of tension and high mental stress frequently along with the fast pace of life and study; or oral ulcer, which is a civilized disease, frequently occurs due to bad living habits, and is common to cold. It usually grows on lips, tongue or other parts of oral cavity, and can recur frequently, with good time and bad time, and is not classified into men and women, old and young. The flora in the oral cavity is symbiotic with the host, generally, the oral cavity is in a balanced state, if the balanced oral flora is damaged, oral diseases including oral ulcer can occur, and the change of the contents of streptococcus, veillonella flora and neisseria flora in the oral ulcer can directly influence the oral micro-ecological balance.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: aiming at the defects and the defects of single effect and unsatisfactory effect of the existing products for adjusting oral flora, thereby preventing and treating halitosis, treating canker sore or relieving periodontitis, the invention provides a traditional Chinese medicine composition for adjusting oral flora and a preparation method of an active extract thereof. The invention selects and uses the elsholtzia splendens, chrysanthemum and honeysuckle which are medicinal and edible Chinese medicinal materials to be compatible with the astragalus root as the Chinese medicinal composition for preventing and treating the peculiar smell of the oral cavity, and combines the Chinese medicaments according to the dialectical theory of the etiology of the Chinese medicament and the compatibility and medication rule of the Chinese medicaments to strengthen the oral cavity nursing effect, and the raw materials play the roles of preventing and adjusting the oral flora together through the monarch, minister, assistant and guide compatibility theory, thereby improving the oral cavity condition. The traditional Chinese medicine composition or the traditional Chinese medicine compound can effectively prevent and treat oral diseases such as oral ulcer and periodontal disease caused by oral bacterial imbalance or certain pathogenic bacteria, and has remarkable oral care effect.
The invention adopts the following technical scheme to achieve the purpose of the invention.
Firstly, the invention discloses a traditional Chinese medicine composition for adjusting oral flora, which is a direct mixture or an active extract of traditional Chinese medicine raw materials.
The traditional Chinese medicine composition comprises the following components in parts by mass: 20-30 parts of elsholtzia, 20-25 parts of astragalus, 10-20 parts of chrysanthemum morifolium and 5-10 parts of honeysuckle.
Herba Moslae is Labiatae plant herba MoslaeMoslachinensisMax-imakOr herba MoslaeM.chinensis- jiangxiangru．Dried aerial parts of (1). It is recorded in the Ming Yi Bie Lu (miscellaneous records of famous physicians), listed as a middle-grade food, and has the actions of inducing sweat to relieve exterior syndrome, inducing diuresis to alleviate edema, regulating the middle warmer to resolve dampness, etc., with pungent and mild taste. Herba Moslae chinensis is used as genuine herb of herba Moslae chinensis, and hasThe modern researches show that the elsholtzia haichowensis contains various chemical components and has the pharmacological effects of relieving fever, resisting inflammation, etc. Elsholtzia splendens is an important component of the Chinese medicinal composition, contains aromatic substances which not only have direct killing effect on bacteria, anaerobic bacteria, viruses and acid-producing enzyme in the mouth, but also can inhibit the activity of the bacteria, the viruses, the anaerobic bacteria and the acid-producing enzyme, and accords with the modern proposed dental plaque-adjusting microecological balance theory (Zhoudoudong, Wao, Li Jio. the new concept of ecological prevention and control of dental plaque [ J]The division of foreign medicine into oral medicine 1997(06):323-326; billow. dental plaque microecology and its significance in oral diseases [ J]The division of foreign medicine and oral medicine, 1996(02): 104-.
Radix astragali, also known as Wangsun, BAIYAO, and radix astragali, is the dried root of Astragalus membranaceus (Fisch.) bge. var. mongholicus (Fisch.) bge. or Astragalus membranaceus (Fisch.) bge. var. mongholicus. Sweet in nature and taste, slightly warm, and has effects of invigorating spleen and replenishing qi, arresting sweating, inducing diuresis to alleviate edema, removing toxic substance and promoting granulation. The astragalus membranaceus is widely applied in clinical traditional Chinese medicine.
Hangzhou chrysanthemum, pungent, sweet, bitter and slightly cold, can disperse wind and heat, calm liver and improve eyesight, clear away heat and toxic material, has complete inhibition effect on escherichia coli, dysentery bacillus, typhoid bacillus, paratyphoid bacillus, proteus, pseudomonas aeruginosa, vibrio cholerae and other bacteria, and also has some inhibition effect on tubercle bacillus of human body.
Honeysuckle flower, flos Lonicerae, sweet in nature and cold in nature, is fragrant, sweet in nature and cold in nature, clears heat without hurting stomach, and is fragrant and capable of eliminating pathogenic factors. The honeysuckle can disperse wind-heat and clear away blood toxin, and has obvious effect on various heat diseases such as fever, eruption, macula, sore and carbuncle due to heat toxin, sore throat and the like.
The invention selects the medicinal and edible Chinese medicinal materials of Jiangxiang Chinese mosla herb, Hangzhou chrysanthemum and honeysuckle flower which are compatible with the astragalus root to be used as the traditional Chinese medicine composition for adjusting oral flora and preventing and treating oral peculiar smell, wherein the Jiangxiang Chinese mosla herb is a monarch drug for inducing diuresis to alleviate edema, regulating the middle warmer to eliminate dampness and diminishing inflammation; astragalus root, radix astragali induces diuresis to alleviate edema, removes toxicity and promotes granulation, is a medicament for assisting the monarch drug Jiangxiang elsholtzia to strengthen the treatment of main diseases and main symptoms, and is a medicament which has a treatment effect on concurrent diseases or concurrent symptoms, for example, the astragalus root, the elsholtzia root and the elsholtzia root can improve the problem of excessive absorption of alveolar bone caused by inflammation; the chrysanthemum morifolium ramat has the effects of clearing heat, removing toxicity and resisting bacteria, and is used for assisting the monarch drug of the elsholtzia and the ministerial drug of the astragalus to strengthen the treatment effect and directly treating the secondary concurrent syndrome; honeysuckle disperses wind-heat, clears blood toxin, has fragrance and can eliminate pathogenic factors, is a medicine for inducing channels in the recipe, leads various medicines in the recipe to directly reach the disease, and can coordinate the medicines to ensure that the medicines jointly act to eliminate pathogenic factors. The raw materials are compatible and play a role in preventing and adjusting oral flora together, thereby improving the oral condition. The traditional Chinese medicine composition or the traditional Chinese medicine compound can effectively prevent and treat oral diseases such as oral ulcer and periodontal disease caused by oral bacterial imbalance or certain pathogenic bacteria, and has remarkable oral care effect.
Further, the oral flora adjustment method comprises the following steps: eliminating halitosis caused by harmful bacteria in oral cavity, preventing and treating oral ulcer or relieving periodontitis.
Further, the direct mixture of the traditional Chinese medicine composition is used for preparing a medical preparation for adjusting oral flora, preventing and treating oral peculiar smell, preventing and treating oral ulcer or relieving periodontitis.
Furthermore, the active extract of the traditional Chinese medicine composition is used for preparing medical preparations, medical articles, food therapy products and daily chemical products with the functions of adjusting oral flora and improving oral hygiene.
Furthermore, the medical preparation can be powder, granules, capsules and the like.
Furthermore, the medical articles comprise gargle, chewable tablets, buccal tablets, effervescent tablets, popping candy and the like.
Furthermore, the food therapy product comprises beverage, biscuit, yoghourt, jelly and the like.
Furthermore, the daily chemical products comprise medicinal toothpaste and the like.
Secondly, the invention also discloses a preparation method of the traditional Chinese medicine combined active extract for adjusting the oral flora.
The preparation method comprises the following steps: s1, crushing the traditional Chinese medicine raw materials in the traditional Chinese medicine composition; s2, mixing the crushed traditional Chinese medicine raw materials uniformly according to the traditional Chinese medicine combination ratio; s3, soaking the mixed traditional Chinese medicine raw materials in water; s4, decocting the Chinese medicinal materials; s5, filtering to obtain decoction filtrate; s6, concentrating the decoction filtrate; s7, sterilizing, subpackaging and storing for later use.
Further, the water is added in the step S3 for soaking for 0.5-2 h; step S4, decocting the traditional Chinese medicine raw materials for 2-3 times, wherein each time lasts for 0.5-1 h; the concentration of the step S6 is to carry out decompression concentration under the condition of vacuum < -0.08 MPa.
Has the advantages that:
(1) according to the theory of etiology and dialectical theory of traditional Chinese medicine, the elsholtzia splendens induces diuresis to alleviate edema, regulates the middle warmer to eliminate dampness and diminish inflammation; astragalus root, radix astragali induces diuresis to alleviate edema, removes toxicity and promotes granulation; the chrysanthemum morifolium ramat has the effects of clearing away heat and toxic materials and resisting bacteria; honeysuckle flower, flos Lonicerae, with the functions of dispersing wind-heat, clearing away blood-toxin, and eliminating pathogenic factors, can be used for treating rheumatism. The elsholtzia splendens is an important component of the traditional Chinese medicine composition, contains aromatic substances, not only has direct killing effect on bacteria, anaerobic bacteria, viruses and acid-producing enzymes in the mouth, but also can inhibit the activity of the bacteria, the viruses, the anaerobic bacteria and the acid-producing enzymes, accords with the theory of adjusting the microecological balance of dental plaque proposed at present, and has good oral hygiene nursing effect. The elsholtzia splendens is combined with the astragalus, the honeysuckle and the Hangzhou chrysanthemum, so that the effects of clearing heat and removing toxicity and resisting inflammation can be better exerted, dental plaque is inhibited, periodontitis is improved, and excessive and too fast absorption of alveolar bones caused by periodontitis is relieved. The raw materials are compatible and play a role in preventing and adjusting oral flora together, thereby improving the oral condition.
(2) According to the invention, by selecting medicinal and edible traditional Chinese medicinal materials with high safety and carrying out comparative analysis through a large number of safety and pharmacodynamic tests, the product formula and the formula proportion are continuously optimized and screened; the product is subjected to a plurality of detection analysis and experiments, has obvious effects of adjusting oral flora and inhibiting bacteria, high safety, no obvious side effect and high market popularization value.
Drawings
FIG. 1 is a photomicrograph showing the effect of the active extract A of the Chinese medicinal composition on alveolar bone of a rat with periodontitis.
Detailed Description
The present invention will be further described with reference to specific examples, but the present invention is not limited to the following examples. The method is a conventional method unless otherwise specified. The starting materials are commercially available from the open literature unless otherwise specified.
Example 1: preparation of active extract A of Chinese medicinal composition
The weight ratio is as follows: 25 parts of elsholtzia splendens, 22.5 parts of astragalus, 15 parts of chrysanthemum morifolium and 7.5 parts of honeysuckle flower, and firstly, crushing the traditional Chinese medicine raw materials (dry products); secondly, uniformly mixing the crushed traditional Chinese medicine raw materials and adding water to soak for 1 hour; decocting the soaked Chinese medicinal materials with water for 3 times (1 hr, 0.5 hr, and 0.5 hr for three times), and filtering to obtain decoction filtrate; then merging the decoction filtrates, and carrying out vacuum concentration under the vacuum condition of-0.08 MPa until the liquid medicine contains 10 g/ml of crude drug, and stopping concentration; and finally sterilizing, subpackaging and storing for later use to obtain the traditional Chinese medicine composition active extract A.
Example 2: preparation of active extract B of Chinese medicinal composition
The weight ratio is as follows: 30 parts of elsholtzia splendens, 20 parts of astragalus membranaceus, 20 parts of chrysanthemum morifolium and 5 parts of honeysuckle, and firstly, crushing the traditional Chinese medicine raw materials (dry products); secondly, uniformly mixing the crushed traditional Chinese medicine raw materials, and adding water to soak for 2 hours; decocting the soaked Chinese medicinal materials with water for 2 times (1 hr and 1 hr for the second time), respectively, and filtering to obtain decoction filtrate; then merging the decoction filtrates, and carrying out vacuum concentration under the vacuum condition of-0.08 MPa until the liquid medicine contains 10 g/ml of crude drug, and stopping concentration; and finally sterilizing, subpackaging and storing for later use to obtain the traditional Chinese medicine composition active extract B.
Example 3: preparation of active extract C of Chinese medicinal composition
The weight ratio is as follows: 20 parts of elsholtzia splendens, 25 parts of astragalus membranaceus, 10 parts of chrysanthemum morifolium and 10 parts of honeysuckle flower, and firstly, crushing the traditional Chinese medicine raw materials (dry products); secondly, uniformly mixing the crushed traditional Chinese medicine raw materials and adding water to soak for 0.5 h; decocting the soaked Chinese medicinal materials with water for 2 times (1 hr and 0.5 hr for the second time), and filtering to obtain decoction; then merging the decoction filtrates, and carrying out vacuum concentration under the vacuum condition of-0.08 MPa until the liquid medicine contains 10 g/ml of crude drug, and stopping concentration; and finally sterilizing, subpackaging and storing for later use to obtain the traditional Chinese medicine composition active extract C.
Example 4: bacteriostasis experiment of traditional Chinese medicine combined active extract A on 9 standard strains
The active extract A of the traditional Chinese medicine composition obtained in example 1 is diluted to a required concentration by sodium chloride injection, the test tube double dilution method is adopted to examine the bacteriostasis of the extracts of the compositions with different concentrations to 9 standard strains (staphylococcus aureus, escherichia coli, candida albicans, pseudomonas aeruginosa, klebsiella pneumoniae, fusobacterium nucleatum, streptococcus mutans, actinomyces viscosus and prevotella intermedia), and the minimum bacteriostasis concentration is measured. And simultaneously, metronidazole injection and stomatitis spray (Heilongjiang Tianlong pharmaceutical Co., Ltd.) are selected as comparison. The results are shown in table 1:
table 1: comparison of the bacteriostatic effects on aerobic bacteria
From the experimental results of table 1, it can be seen that: the traditional Chinese medicine composition active extract A has a remarkable bacteriostatic action on aerobic bacteria and anaerobic bacteria. Wherein, the bacteriostatic effect on Escherichia coli, Candida albicans and Klebsiella pneumoniae is obviously better than that of the stomatitis spray; the bacteriostatic effect on streptococcus mutans and prevotella intermedia is obviously better than that of metronidazole injection; the bacteriostatic effect on staphylococcus aureus and pseudomonas aeruginosa is basically consistent with that of the stomatitis spray; only has slightly worse bacteriostatic effect on fusobacterium nucleatum and actinomyces viscosus than metronidazole injection.
Example 5: healing experiment of traditional Chinese medicine composition active extract A on experimental oral ulcer
The active extract a of the traditional Chinese medicine composition obtained in example 1 was diluted to a desired concentration with sodium chloride injection to perform an experiment for healing of experimental oral ulcer. The experimental design, the process implementation and the experimental results are as follows:
40 rats were randomly divided into a model control group, a Bingpeng tablet group (positive control group) of Dongtai pharmaceutical company Limited liability company of Baiyunshan Tang Yin, a large dose group of the combination, and a small dose group of the combination, 4 groups, each group containing 10 rats. Each group of rats was anesthetized with ether and fixed supine on a rat plate. A glass tube with the diameter of 4 mm is taken, a small cotton ball is placed at one end of the glass tube to be flush with the tube opening, the cotton ball end of the glass tube is soaked in phenol solution with the mass fraction of 900 g/L to enable the liquid medicine to soak the cotton ball, and the cotton ball is placed on the buccal mucosa of one side of a rat to be burnt for about 60 s. The glass tube is removed, white damage with the diameter of 4-6 mm can be seen in the area, and the lips of the rat mouth can be seen to be moist, the mouth can flow into water, the buccal mucosa is red and swollen by naked eyes the next day, namely the oral ulcer modeling is successful.
In the large-dose group of the combination, 1250 mu L of the combination is given per 100g of body mass (equivalent to 15 times of the daily dosage of clinical people), and the combination is averagely dropped into the ulcer part of the buccal mucosa of a rat for 5 times at intervals of 2 hours.
In the small-dose group of the combination, 415 mu L of the combination is given per 100g of body mass (which is 5 times of the daily dosage of clinical people), and the combination is averagely dropped into the ulcer part of the buccal mucosa of a rat for 2 times at intervals of 4 hours.
Ice boron tablet group: the Bingpeng buccal tablet powder is 1.5 mg/g, and is placed on the ulcer of the buccal mucosa of a rat at an interval of 2h in 5 times on average.
The model control group was not administered.
For the large dose group, the small dose group and the Bingpeng tablet group, after each administration, fasting and water prohibition are performed for 30 min, and the continuous administration is performed for 6 d. The healing of the canker sores in the rats was observed before daily dosing, and the size of the diameter of the canker sore surface (in mm) and the time and amount of healing of the canker sores in the rats were recorded 6 days after dosing. The healing standard is that the ulcer with the diameter less than 1mm is healed, and the ulcer with the diameter more than or equal to 1mm is not healed. The results are shown in Table 2. Compared with a model control group, when the combination is continuously administrated for 6 d, the diameter of the oral ulcer surface of rats in the large and small dose groups of the combination is reduced, the number of the rats with the healed oral ulcer is increased, the difference has statistical significance (P is less than 0.01 or P is less than 0.05), and the results are shown in a table 2:
TABLE 2 comparison of the diameter of the oral ulcer surface of each group of rats x + -s
※Compared with the model group, the difference is significant (P is less than 0.05),﹟the difference is significant compared with a positive control group (P is less than 0.05)
From the experimental results of table 2, it can be seen that: after the experimental rat oral ulcer model is successfully manufactured and the medicine is applied for treatment, the ulcer area is reduced by the traditional Chinese medicine combination active extract A and the positive medicine group, and the difference is obvious (P is less than 0.05). By the 6 th day, the high dose of the traditional Chinese medicine combination group achieves the significant difference (P is less than 0.01) and the difference with the positive medicine is significant (P is less than 0.05), which shows that the traditional Chinese medicine combination can obviously promote the healing of experimental oral ulcer of rats.
Example 6: in-vitro bacteriostasis test of traditional Chinese medicine composition active extract A
The active extract A of the traditional Chinese medicine composition obtained in the example 1 is diluted to a required concentration by sodium chloride injection, and the in vitro bacteriostasis of staphylococcus aureus, escherichia coli and candida albicans is tested by adopting a plate perforation and drug filling method. The experimental design, the process implementation and the experimental results are as follows:
taking 7 sterilized test tubes, numbering and arranging on a test tube rack, adding 3 mL of 1000 g/L of the traditional Chinese medicine composition A into the 1 st tube, adding 3 mL of sterile physiological saline into the 2 nd to 4 th tubes respectively according to a sterile operation method, sucking 3 mL of the 1000 g/L of the traditional Chinese medicine composition A into the 2 nd tube, uniformly mixing, sucking 3 mL out of the 1 st tube, adding the 3 rd tube, uniformly mixing, sucking 3 mL out of the 3 rd tube, adding the 3 rd tube into the 4 th tube, and uniformly mixing. Add norfloxacin tablet 2 mg/mL in tube 5, ketoconazole tablet 1 mg/mL in tube 6, and sterile physiological saline in tube 7. Heating and melting a label sterilization plate, then cooling to 20 mL of a beef extract soup agar culture medium (a Sha's culture medium for Candida albicans) at 40 ℃, shaking uniformly, after solidification, uniformly punching (3-4 holes/plate) on the culture medium by using an aseptic puncher with the diameter of 6 mm, respectively adding the prepared liquid medicine and physiological saline (0.2 mL/hole) with different mass fractions into each hole, respectively and uniformly inoculating the cultured staphylococcus aureus, Escherichia coli and Candida albicans around each hole, incubating for 24 h at 37 ℃, and observing and measuring the diameter of an inhibition zone. The above operation was repeated twice, i.e. each tube was made 3 parallels, and the average of the diameters of the last zone of inhibition was taken, the results are shown in table 3:
TABLE 3 in vitro bacteriostatic diameter results (mm) of the active extract A of the Chinese medicinal composition
From the experimental results of table 3, it can be seen that: the Chinese medicinal composition active extract A has obvious in-vitro bacteriostatic action on staphylococcus aureus, escherichia coli and candida albicans.
Example 7: irritation experiment of traditional Chinese medicine combined active extract A on rat oral mucosa
The active extract A of the traditional Chinese medicine composition obtained in the example 1 is diluted to a required concentration by sodium chloride injection, a stimulation experiment is carried out on the oral mucosa of a rat, and the stimulation response of multiple times of administration to the oral mucosa of the rat is observed. The experimental design, the process implementation and the experimental results are as follows:
20 rats are randomly divided into 2 groups of the traditional Chinese medicine combination group and a normal control group, and each group comprises 10 rats. The traditional Chinese medicine composition is uniformly dropped into the buccal mucosa of both sides of a rat for 5 times within 4 hours, wherein the mass of the traditional Chinese medicine composition is 2500 mu L/100g (equivalent to 30 times of the daily dosage of clinical people). The normal control group was not treated at all. After 24 h of administration, the congestion and edema of the administration site are observed and recorded, and the animals are scored to see whether the animals have systemic adverse reactions. The administration and observation were carried out daily for 7 days, and the observation was continued for 7 days after the withdrawal of the drug. The mucous membrane congestion and edema scoring standards are shown in tables 4 and 5, and the results are shown in table 6;
TABLE 4 mucosal irritant response and scoring criteria
TABLE 5 evaluation criteria for mucosal irritation Strength
Table 6 mean oral mucosa irritation response score n =10 after multiple dosing for each group
From the experimental results of table 6, it can be seen that: the active extract A of the traditional Chinese medicine composition has no irritation to the oral mucosa of a rat, and is a safe traditional Chinese medicine composition.
Example 8: influence of traditional Chinese medicine composition active extract A on curative effect and microbial flora of oral ulcer patients
1. Study Subjects and screening principles
50 patients with oral ulcer collected during the period from 10 months in 2018 to 4 months in 2019 are taken as study objects and are randomly divided into an observation group and a control group, and each group comprises 25 patients. Wherein 15 male cases and 10 female cases are selected as a control group; the age is 27-58 years, the mean age (41.8 + -15.4 years); the course of disease is 20-47 days, and the average course of disease (31.2 + -16.7 days). 13 men and 12 women in the Chinese medicinal composition observation group; age 25-54 years, mean age (42.9 ± 13.2) years; the course of disease is 23-50 days, and the average course of disease (32.1 + -18.2 days). The general data of two groups of patients has no significant difference (P > 0.05) and is comparable. (1) All patients meet the diagnosis standard of oral ulcer in oral mucosa pathology; (2) the traditional Chinese medicine diagnosis conforms to aphtha patients in the clinical research guidelines of new traditional Chinese medicines of the Ministry of health; (3) those who do not use Chinese medicines in about 6 months; (4) the patients who do not use hormone medicines in about 6 months; and (5) obtaining informed consent of the patients and family members thereof.
2 method of treatment
2.1 control group
Applying levamisole liniment (Fuzhou Chenxing pharmaceutical Co., Ltd., Chinese medicine standard H20003344) every other day for 500 mg/time for 2 weeks; vitamin C tablet (Shenyang first pharmaceutical Co., Ltd, national Standard H21022862 of northeast China pharmaceutical Co., Ltd.) is administered orally at a dose of 0.2 g/time, 3 times daily. Vitamin B12 (Shanghai Xinyijiufu pharmaceutical Co., Ltd., national drug Standard H31022370) was orally administered at 10 mg/time, 3 times daily.
2.2 Observation group
The traditional Chinese medicine composition is adopted for treatment, the extract is baked to be dry, the extract is ground into powder and put into a plastic small bottle with a spray head, the ulcer wound surface is sprayed for 3 times/d, and water or food does not need to be drunk within at least 1 hour after the medicine is sprayed.
The efficacy was assessed after 14 days of treatment in both groups.
3 therapeutic results
3.1 therapeutic efficacy criteria
And (3) healing: all clinical symptoms disappeared; the effect is shown: the ulcer wound surface is healed, and the pain is eliminated; the method has the following advantages: the wound surface of the ulcer is reduced by more than or equal to 1/2, and the pain is not obvious; and (4) invalidation: the symptoms did not improve or the ulcer area decreased <1/3 as before, and the patient still did not eat food smoothly.
3.2 Observation index
Before and after treatment, the curative effect of two groups of patients is evaluated respectively, and the ulcer duration is counted. The number of oral streptococci, neisseria and veillonella in two groups of patients was determined by PCR fluorescence before and after treatment.
3.3 results
3.3.1 the curative effect of two groups of patients is compared: see table 7.
TABLE 7 two comparative therapeutic effects (examples)
P <0.05 compared to control group.
3.3.2 comparison of ulcer duration in two groups of patients: see table 8.
TABLE 8 comparison of ulcer durations (d, x + -s)
P <0.05 compared to control group.
3.3.3 two groups of oral microbial flora comparison: see table 9.
TABLE 9 two oral microbial flora comparisons (one, x + -s)
P <0.05 compared to group before treatment; # P <0.05 compared to control after treatment.
According to the experimental observation of the population, the traditional Chinese medicine composition active extract A has a better effect on the oral ulcer, which is shown in that the total effective ratio of the extract A to the ulcer is obviously improved compared with the illumination, the healing time of the ulcer is shortened, beneficial bacteria related to the oral ulcer are promoted, and the condition of flora imbalance is improved.
Example 9: effect of the active extract A of the Chinese medicinal composition on alveolar bone of a rat with periodontitis
48 SD rats with complete dentition, no caries and periodontal disease of 5 weeks old are selected, and the SD rats are half male and female and have the body weight of 200g +/-20 g. Randomly dividing into a normal control group, a model group and a Koutai group (a positive treatment group, the used medicine is compound chlorhexidine gargle, Jiangsu Cheng brand bond pharmaceutical industry Co., Ltd., Chinese medicine standard character H20058018), and a traditional Chinese medicine composition group A (divided into a low-medium group according to the dosage): composition A low dose group was 0.5mg/kg, composition A medium dose group was 1.5mg/kg, and composition A high dose group was 4.5mg/kg, with 8 rats per group. Improved by the Luoxijun literature method (Luoxijun, beam, Liverun, Xiyufeng, Qiaofiwei. creation of periodontitis animal model [ J ]. journal of oral Material instruments, 2005(03): 120-. Cutting off the gum around two teeth with a pair of large forceps, cutting off with ophthalmic curved scissors, feeding soft feed soaked with 10% white granulated sugar water, and feeding with normal diet after molding, and treating, wherein each animal is administered with 0.1ml/100g oral cavity twice daily for administration, and feeding with water after fasting for 4 hr. After the experiment, the alveolar bone of the animal is taken to be pathological section to observe the status of the alveolar bone (see attached figure 1).
From the attached FIG. 1, it can be seen that: the composition A obviously inhibits the alveolar bone resorption of experimental rat periodontitis.
Similarly, the combined active extracts B, C of the herbs obtained in examples 2 and 3 were tested according to the experimental methods and procedures described in examples 4-9, and the results showed that: the experimental data are similar to those of the examples 4-9, and the experimental conclusion is consistent with those of the examples 4-9.
The embodiments of the present invention have been described in detail, but the embodiments are merely examples, and the present invention is not limited to the above-described embodiments. Any equivalent modifications and substitutions to those skilled in the art are also within the scope of the present invention. Accordingly, equivalent alterations and modifications are intended to be included within the scope of the invention, without departing from the spirit and scope of the invention.
Claims (6)
1. A traditional Chinese medicine for adjusting oral flora is characterized in that: is prepared from 20 to 30 portions of elsholtzia haichowensis, 20 to 25 portions of astragalus, 10 to 20 portions of chrysanthemum and 5 to 10 portions of honeysuckle flower; the preparation method of the traditional Chinese medicine comprises the following steps: s1, crushing the traditional Chinese medicine raw materials in the traditional Chinese medicine composition; s2, mixing the crushed traditional Chinese medicine raw materials uniformly according to the traditional Chinese medicine combination ratio; s3, soaking the mixed traditional Chinese medicine raw materials in water; s4, decocting the Chinese medicinal materials; s5, filtering to obtain decoction filtrate; s6, concentrating the decoction filtrate; s7, sterilizing, subpackaging and storing for later use.
2. The traditional Chinese medicine for regulating oral flora according to claim 1, wherein the regulation of oral flora is: eliminating halitosis caused by harmful bacteria in oral cavity, preventing and treating oral ulcer or relieving periodontitis.
3. The traditional Chinese medicine for modulating oral flora of claim 1, wherein: can be used for preparing medical preparation, health food, and daily chemical product with effects of regulating oral flora and improving oral hygiene.
4. The traditional Chinese medicine for modulating oral flora of claim 1, wherein: the water is added for soaking for 0.5-2 h in the step S3.
5. The traditional Chinese medicine for modulating oral flora of claim 1, wherein: in step S4, the Chinese medicinal materials are decocted for 2-3 times, each time for 0.5-1 h.
6. The traditional Chinese medicine for modulating oral flora of claim 1, wherein: the concentration of the step S6 is to carry out decompression concentration under the condition of vacuum < -0.08 MPa.
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| CN112807343B (en) * | 2021-01-22 | 2022-04-19 | 天津中医药大学 | Oral flora polymorphism regulator |
| CN116251160B (en) * | 2023-04-21 | 2023-09-22 | 成都长哲健康科技有限公司 | Pharmaceutical composition with antibacterial effect and preparation method and application thereof |
| CN116492369B (en) * | 2023-06-27 | 2023-09-08 | 江西草珊瑚个人护理用品有限公司 | Traditional Chinese medicine polysaccharide composition with anti-inflammatory and hemostatic effects and application thereof |
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